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GenLNP-S04-EGFP mRNA (m1Ψ)

RP-A00012
$300.00

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Description

The GenLNP-S04-EGFP mRNA (m1Ψ) is a CD34 antibody decorated LNP loaded with eGFP mRNA (m1Ψ). It displays anti-human CD34 antibody mAb, Mouse (GenScript, Cat: CP0001) to enhance its targeting efficiency to CD34 positive cells. The antibody is covalently conjugated to lipid molecule for achieving targeting effect.  
 
The LNP is formulated with main ionizable lipid of SM102.  
 
The payload eGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). This LNP formulation showed enhanced delivery of eGFP mRNA to CD34+ stem cells.
Properties
Form Liquid
Concentration 0.15mg/mL
Full mRNA length 980nt
Full mRNA Molecular Weight 319792
Storage Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.
Storage buffer PBS/Sucrose

Quality Control Specifications
Appearance Clear and free of foreign particles
Encapsulation Efficiency > 85%
Endotoxin < 4 EU/ml
LNP Particle Size 65-165 nm
pH 7.4 ± 0.5​
Polydispersity Index < 0.2
Zeta Potential ±20 mV

Applications
1. LNP can be added directly to cells incubated in complete media 
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium 
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate  
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression 
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.