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EGFP MRNA (Cap1, M1Ψ)

Capping efficiency test of eGFP mRNA by LC-MS. Capped eGFP mRNA fragment has a M/Z of 8473, uncapped mRNA fragment has a M/Z of 8116. The capped fragment ratio is 99.5%.

EGFP MRNA (Cap1, M1Ψ)

Expression of eGFP mRNA in A549 cells. Confocal microscopy revealed that efficient expression of eGFP mRNA in A549 cells after transfection with lipofectamine 2000. Green: eGFP protein, Blue: Nuclei.

eGFP mRNA (Cap1, m1Ψ)

The eGFP mRNA encodes an enhanced green fluorescent protein, originally derived from the jellyfish, Aequorea victoria. eGFP mRNA is widely used as a fluorescent reporter in mRNA-based research, especially in therapeutic development and delivery system validation.
RP-A00009
$300.00

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Description

The eGFP mRNA encodes an enhanced green fluorescent protein, originally derived from the jThe eGFP mRNA encodes an enhanced green fluorescent protein, originally derived from the jellyfish, Aequorea victoria. eGFP mRNA is widely used as a fluorescent reporter in mRNA-based research, especially in therapeutic development and delivery system validation. The expressed eGFP protein fluoresces green, with excitation at 488 nm and emission at 507 nm.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 980nt
Full mRNA Molecular Weight 319792
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
Capping Efficiency ≥ 90%
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
pH Target ± 0.5
Endotoxin < 10 EU/mg

Applications
Transfect 0.1-0.2 µg of mRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Analyze the expression 16-48 hours post-transfection using a plate reader, flow cytometer, fluorescence microscope, or confocal microscope. For equipment settings, we recommend: 485 nm excitation, 535 nm emission.

Examples
  • EGFP MRNA (Cap1, M1Ψ)
    • EGFP MRNA (Cap1, M1Ψ)

    Capping efficiency test of eGFP mRNA by LC-MS. Capped eGFP mRNA fragment has a M/Z of 8473, uncapped mRNA fragment has a M/Z of 8116. The capped fragment ratio is 99.5%.

  • EGFP MRNA (Cap1, M1Ψ)
    • EGFP MRNA (Cap1, M1Ψ)

    Expression of eGFP mRNA in A549 cells. Confocal microscopy revealed that efficient expression of eGFP mRNA in A549 cells after transfection with lipofectamine 2000. Green: eGFP protein, Blue: Nuclei.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.