eGFP mRNA (Cap1, 5-MOU)

Cat. No.	RP-A00008
Size	0.2 mg Buy in bulk
Quantity
Price	$300.00


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Description

The eGFP mRNA encodes an enhanced green fluorescent protein, originally derived from the jellyfish, Aequorea victoria. eGFP mRNA is widely used as a fluorescent reporter in mRNA-based research, especially in therapeutic development and delivery system validation. The expressed eGFP protein fluoresces green, with excitation at 488 nm and emission at 507 nm.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with 5’-Methoxyuridine (5-MOU) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

This construct offers strong, reliable fluorescent signaling, making it ideal for transfection optimization, biodistribution studies, and LNP delivery validation.

Properties

Form	Liquid
Concentration	1mg/mL
Full mRNA length	980nt
Full mRNA Molecular Weight	324468
Storage	Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer	1mM Sodium citrate, pH6.5

Quality Control Specifications

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

Applications

Transfect 0.1-0.2 µg of mRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Analyze the expression 16-48 hours post-transfection using a plate reader, flow cytometer, fluorescence microscope, or confocal microscope. For equipment settings, we recommend: 485 nm excitation, 535 nm emission.

Examples

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

eGFP mRNA (Cap1, 5-MOU)

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