Company News » Human Recombinant CLDN18.2 Stable Cell Line
HEK293/CLDN18.2 Stable Cell Line

FACS analysis of CLDN18.2 expression in HEK293/ CLDN18.2 clone by antiCLDN18.2, compared with blank control (green line, untreated cell) and negative control (blue line, only treated with goat anti-human antibody). The cells analyzed are live cells (E1 gate).

HEK293/CLDN18.2 Stable Cell Line

Stability testing of CLDN18.2 expression in HEK293/ CLDN18.2 clone.for 20 passages by anti-CLDN18.2, compared with blank control (blue line, untreated cell) and negative control (purple line, treated with goat anti-human antibody)

HEK293/CLDN18.2 Stable Cell Line

Check Full-length Claudin 18.2 recombinant protein Z03504.

Claudins are a family of proteins, which play a role in maintaining tight cell junctions. Differentclaudin subtypes are expressed on different tissues.Claudin18 isoform 2 (CLDN18.2) expressionin normal tissues is restricted to cells of the gastric mucosa and is absent from other healthytissues However, CLDN 18.2 is expressed in 70% of primary gastric adenocarcinomas and itsmetastases (101). It has also beenshown to be expressed in a number of other cancers,including pancreatic (50%), esophagus (30%) and NSCLC (25%)The restricted expressionmakes it a potential drug target for the treatment of gastric and pancreatic adenocarcinoma, asevidenced by effortsto target CLDN18.2 via naked antibody and CAR-T modalities.
M00917
$8500.00

Ask us a question
Description

Claudins are a family of proteins, which play a role in maintaining tight cell junctions. Differentclaudin subtypes are expressed on different tissues.Claudin18 isoform 2 (CLDN18.2) expressionin normal tissues is restricted to cells of the gastric mucosa and is absent from other healthytissues However, CLDN 18.2 is expressed in 70% of primary gastric adenocarcinomas and itsmetastases (101). It has also beenshown to be expressed in a number of other cancers,including pancreatic (50%), esophagus (30%) and NSCLC (25%)The restricted expressionmakes it a potential drug target for the treatment of gastric and pancreatic adenocarcinoma, asevidenced by effortsto target CLDN18.2 via naked antibody and CAR-T modalities.

* The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit of Lonza.
Synonyms

Claudin-18 isoform A2

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Overview
Applications Binding assay or use as immunogen

Product Introduction
Storage Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. We recommend immediate resuscitation upon receipt of the cells
QC Mycoplasma negative
Stability Stable expression of CLDN18.2 for more than 15 passages

Culture Conditions
Freeze Medium 49% DMEM, 45% FBS, 6% (V/V) DMSO
Culture Medium DMEM, 10% FBS,500μg/ml(G418 Sulfate) Geneticin (G418 Sulfate)

Examples
  • HEK293/CLDN18.2 Stable Cell Line
    • HEK293/CLDN18.2 Stable Cell Line

    FACS analysis of CLDN18.2 expression in HEK293/ CLDN18.2 clone by antiCLDN18.2, compared with blank control (green line, untreated cell) and negative control (blue line, only treated with goat anti-human antibody). The cells analyzed are live cells (E1 gate).

  • HEK293/CLDN18.2 Stable Cell Line
    • HEK293/CLDN18.2 Stable Cell Line

    Stability testing of CLDN18.2 expression in HEK293/ CLDN18.2 clone.for 20 passages by anti-CLDN18.2, compared with blank control (blue line, untreated cell) and negative control (purple line, treated with goat anti-human antibody)