Identification of “Hot Spots” in the CDRs of A SARS-CoV2 Antibody by Alanine Scanning

GenScript’s arrayed alanine scanning libraries enable researchers to rapidly identify the essential amino acid residues responsible for a specific protein function due to their sequence-verified and ready-to-use deliverable format. In this application note, we harnessed the power of the alanine scanning mutagenesis tool and demonstrated the utility of our arrayed alanine scanning library in an effort to rapidly identify amino-acid residues within the complementarity-determining regions (CDRs) of an antibody of the SARS-CoV2 virus that are essential for binding its target antigen, the receptor-binding domain (RBD) of the viral Spike (S) protein.