Catalog Products » Protein Isolation and Purification » Protein Purification Resins » Anti-DYKDDDDK G1 Affinity Resin

Anti-DYKDDDDK G1 Affinity Resin

DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. GenScript Anti-DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate. Table 1 lists the main characteristics of Anti-DYKDDDDK G1 Affinity Resin.
L00432
$281.00

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Description

DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. GenScript Anti-DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate. Table 1 lists the main characteristics of Anti-DYKDDDDK G1 Affinity Resin.
Overview
Key Features

Key Features
  • High DYKDDDDK binding capacity and ultralow levels of non-specific binding
  • Multiple elution methods for diverse protein characteristics and different requirements
  • Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Characteristics:
Product content 50% settled resin in TBS with 0.02% sodium azide

Matrix

4% cross-linked agarose

Average bead size

90 μm

Ligand

Mouse monoclonal antibody against DYKDDDDK tag, clone G1

Binding capacity

Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin

Storage and stability

Store at 2-8 °C for up to 12 months. Do not freeze the resin.

Resin reuse

The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.

Elution method

Acid, alkaline, neutral, peptide competitive or PAGE loading buffer

NOTE:Elution with SDS-PAGE loading buffer causes denaturation of the G1 antibody.

Reagents compatibility

Compatible with commonly used reagents at certain concentrations

Comparison between Anti-DYKDDDDK IP Resin and Anti-DYKDDDDK G1 Affinity Resin.
Anti-DYKDDDDK G1 Affinity Resin (L00432) Anti-DYKDDDDK IP Resin (L00425)
Immunoprecipitation (IP) √ √
Purification √ n/a
Elution Alkaline elution √ n/a
Alkalescent elution √ n/a
Acid elution √ n/a
Neutral elution √ n/a
Competitive elution √ n/a
SDS-PAGE loading synthesize buffer √ √
Regeneration 4-10 times not recommend
Binding volume large scale mini-volume (<1ml)