CellPower™ Cell Line Development

For Drug Discovery, Toxicity Testing and Basic Research

Overview Assay Cell Line Development CRISPR Cell Line Development

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Assay Cell Line Development

Overview

Cellular assays, or stable cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. The advantages of cell-based assays include the facilitation and generation of complex and biologically relevant data. Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously.

Highlight

250+ cells delivery

Extensive proteins experience

PhD Experts support

Assay Cell Line Workflow

Service Specifications

Service Name	Service Detail	Service Method	Deliverables	Delivery Time
CellPower™ Gene Constitutive Expression	Single gene overexpression stable cell pool/cell line Multiple gene overexpression table cell pool/cell line	Viral Based	2 cryovials of stable cell pool with 10^6 cells per vial 4 cryovials of single-cell-derived stable clones with 10^6 cells per vial (2 clones) Cell pool generation report Cell clones screening report CoA report	Starting from 8 weeks (Cell Pool) Starting from 14 weeks (Cell clone)
CellPower™ Gene Constitutive Expression		Chemical Based
CellPower™ Gene Inducible Expression	Tet-On system Tet-Off system	Viral Based	2 cryovials of stable cell pool with 10^6 cells per vial 2 cryovials of single-cell-derived stable clones with 10^6 cells per vial (1 clones) Cell pool generation report Cell clones screening report CoA report	Starting from 12 weeks (Cell Pool) Starting from 18 weeks (Cell clone)
CellPower™ Gene Inducible Expression	Tet-On system Tet-Off system	Chemical Based
For knockdown cell line, please contact us for further detail.

It is preferred that customers provide their own cell lines, despite available cell lines can be purchased by GenScript for an additional fee. Please note that we currently do not provide genome editing service for primary cells, stem cells or iPS cells.
Validation services for Assay cell lines can be found under the "Add On Services" tab.

Case Study

Therapeutic Anti-Claudin 18.2 Drug Lead Screening

Customer requirement: Generation therapeutic anti-Claudin18.2 antibodies

Claudin 18.2: Tetra-Transmembrane, Gastric tumor marker
Claudin 18.1: Alternatively Spliced, Rich in lung tissue

Project Goal:

To develop Cell Lines expressing Claudin 18.2 or 18.1
Use these Cell Lines as immunogen for monoclonal antibody development and screening purposes

Antigen preparation

Immunization

Hybridoma Formation

Hybridoma Screening

Validation

Cell lines	Application	Purpose
CHO-K1/human Claudin18.2	Antigen	Immunization
HEK293/human Claudin18.2	Hybridoma screening	Positive screening
HEK293/human Claudin18.1	Hybridoma screening	Negative screening
HEK293 parental	Hybridoma screening	Negative screening

CRISPR Cell Line Development

Overview

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. CRISPR sequences were originally identified in the Escherichia coli (E. coli) genome, and were found to function as part of an RNA-based adaptive immune system to target and destroy genetic parasites at the DNA level. CRISPR-associated protein (Cas) is an endonuclease that cuts foreign DNA, allowing integration into the host genome. Cleavage only occurs when there is a protospacer adjacent motif (PAM) around the targeted sequence of the invading DNA, ensuring highly accurate targeting. Researchers studying CRISPR have adapted it for use as a tool for genetic modification of the target host genome. CRISPR/Cas9 has recently become a popular genome editing tool, due to its simplicity and versatility.

CRISPR Cell Line Workflow

Service Specifications

Service Name	Service Detail	Cell Line Options	Deliverables	Delivery Time
GenCRISPR™ EZ knockout Service	Single gene knock out cell pool/cell line Multiplexed gene knock out cell pool/cell line	Available for 90+ popular transfection-suitable cell lines, including A549, CHO-K1, HEK293, HEK293T, HT-29, MDA-MB-231, 4T1, A20, HCT116, MCF7, MDCK, U937, RPMI 8866, etc.	One knockout cell pool/two full-allelic knockout cell lines validated by sequencing One negative control cell pool transfected with non-targeting gRNA Biweekly project updates	3-6 weeks (cell pool) 9-15 weeks (cell clone)
GenCRISPR™ Customized Knockout Service				8-11 weeks (cell pool) 16-24 weeks (cell clone)
GenCRISPR™ Customized Knock-In Service	Point mutation Seamless point mutation Endogenous locus/gene tagging/reporter Cell model for genetic variation study (including SNV, SV, InDel, etc.) Safe harbor insertion of GOI	Any cell line	1-2 knock in cell lines validated by sequencing One negative control cell pool transfected with non-targeting gRNA Biweekly project updates	12-25 weeks (Cell clone)
GenCRISPR™ Whole Gene Deletion Service	Whole gene deletion	Any cell line	One deletion cell pool/full-allelic deletion cell line validated by sequencing One negative control cell pool transfected with non-targeting gRNA Biweekly project updates	13-23 weeks (cell clone)

It is preferred that customers provide their own cell lines, despite available cell lines can be purchased by GenScript for an additional fee. Please note that we currently do not provide genome editing service for primary cells, stem cells or iPS cells.
Validation services for CRISPR cell lines can be found under the "Add On Services" tab.

Case Study

Development of Multiple Gene Knockout Cell Line

A

Validation of A gene KO by RT-PCR

B

Validation of B gene KO by WB

Double gene Knockout

ACLD&CRISPR Add-On Service

Add-On Service	Description
Reverse transcription (RT)-PCR	Validate the positive cell pool/clones at mRNA level
FACS analysis	Validate the transgene expression by FACS analysis. A validated antibody with specific binding to target protein or reporter tag must be provided.
Western Blot	Validate the positive clones by western blot. A validated antibody in the host cells with specific binding to target protein must be provided.
Expression stability test	Validate the GOI expression stability by WB or FACS in 15/20 generation.
One additional clone	QC and deliver one additional positive clone.
*Off-target analysis	Characterize one knockout clone by sequencing the top 5 of potential off-target sites.

*Only suitable for CRISPR cell line development

Resources

CRISPR Webinar

Dr. Theo Roth from Marson Lab, UCSF

introduces the advantages of a new CRISPR based method that allows for the insertion of large DNA sequences (>1Kb) in primary T cells without a virus.

Learn More
White Paper

CRISPR RNP User Manual
A guide on how to use CRISPR RNP for targeted genome editing.
Free Download

CRISPR Handbook CRISPR Handbook
Enabling Genome Editing and Transforming Life Science Research
Free Download