LOC100853412 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100853412
Entrez Gene ID	100853412
Full Name	putative disease resistance RPP13-like protein 1
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019217713.1	XP_019073258.1	putative disease resistance RPP13-like protein 1 isoform X3
XM_019217715.1	XP_019073260.1	putative disease resistance RPP13-like protein 1 isoform X5
XM_019217711.1	XP_019073256.1	putative disease resistance RPP13-like protein 1 isoform X1
XM_019217712.1	XP_019073257.1	putative disease resistance RPP13-like protein 1 isoform X2
XM_019217714.1	XP_019073259.1	putative disease resistance RPP13-like protein 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC100853412 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100853412 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OVa39242	XM_019217711.1 Latest version!	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OVa39243	XM_019217712.1 Latest version!	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OVa39244	XM_019217713.1 Latest version!	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OVa39245	XM_019217714.1 Latest version!	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OVa39246	XM_019217715.1 Latest version!	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100853412 ( XM_019217711.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073256.1 Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa39242
Clone ID Related Accession (Same CDS sequence)	XM_019217711.1
Accession Version	XM_019217711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative disease resistance RPP13-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGATCTTC TAAAGAAACT GAAGAGATAT 
GGAATTTCTG GAATATTATC CTATGGACTC CTGAATACTG CATACTATCT TACAACGTTT 
CTCTTGGTAT GGTTTTATAT TGCTCCAGCA CCAGGAAGGA TGGGTTATTT GGCGGCAGTT 
GAAAGATTTG TCAAAATCAT GGCTATGGTG TGGGCAGGCA GCCAGGTCAC TAAACTTGTC 
AGAGCTGGAG GGGCCCTTGC ACTTGCACCT ATTGTAGATA GAGGATTGTC ATGGTTTACA 
TTAAAATTCA AGTTTGAGTC TCAGGGAAAG TTTGGTGGTA TCGCGGTAAC AGACACCAAT 
CCTCTGAATT CCTATTGCAA AGCAACAGTC GACACAGATA TGGCGGACGT CCTCCTCTCA 
GCTTCGCTTC AAGTTCTATT CGAAAGGTTG GCTTCTCCAG AGCTCATAAA CTTCATTCGG 
CGACGGAACC TTAGCGATGA ACTCCTCAGC GAGTTGAAGA GGAAACTCGT GGTTGTTCTC 
AATGTGCTCG ATGATGCGGA GGTGAAGCAA TTTTCCAACC CAAATGTCAA AGAGTGGCTC 
GTCCATGTCA AGGGTGCTGT GTATGATGCG GAAGACCTGT TGGACGAGAT CGCTACCGAC 
GCTTTGCGTT GCAAGATGGA AGCTGCTGAC TCCCAAACCG GCGGAACTCT TAAGGCGTGG 
AAATGGAACA AGTTCTCTGC TTCTGTGAAG ACTCCATTTG CTATCAAAAG CATGGAGTCC 
AGGGTCAGGG GCATGATTGA TCTACTGGAA AAAATTGCAC TAGAAAAAGT TGGGTTGGGG 
CTGGCAGAGG GTGGGGGCGA GAAACGGTCA CCAAGACCAA GATCACCAAT ATCCACTTCT 
TTGGAGGATG ACTCCATTGT TGTCGGCAGG GATGAAATTC AGAAGGAGAT GGTGGAGTGG 
TTGCTTTCTG ACAATACAAC AGGCGACAAA ATGGGGGTGA TGTCCATAGT GGGCATGGGC 
GGCAGCGGCA AGACCACGCT TGCTCGGCGT CTCTATAACG ATGAGGAAGT GAAGAAACAC 
TTCGACTTGC AAGCATGGGT CTGTGTTTCC ACTGAGTTTC TCCTTATCAA ACTCACCAAA 
ACAATTCTTG AGGAAATTCG TTCTCCACCT ACTTCCGCTG ACAACCTAAA TTTGCTTCAG 
CTTCAACTCA AAGAGCAACT TAGTAACAAG AAATTTCTGC TTGTTCTTGA TGACGTCTGG 
AATTTGAATC CTCGTGATGA AGGTTATATG GAGCTTAGTG ATCGTGAAGG TTGGGAACGT 
CTACGAACTC CACTCCTCGC TGCAGCAGAG GGAAGCAAGA TTGTTGTGAC CAGTCGTAAT 
AAATCTGTTG CAGAAGCCAT GAAAGCGGCC CCTACTCATG ATCTTGGGAA ATTAAGCTCT 
GAAGATAGTT GGTCCCTGTT TAAAAAGCAT GCATTTGGAG ATAGAGACCC GAACGCATTC 
CTTGAGCTTG AACGCATAGG CAGACAGATT GTGGACAAGT GTCAAGGATT GCCTTTGGCT 
GTGAAAGCAC TCGGCTGTCT CTTGTATTCT AAGGACGAAA AAATGGAATG GGACGATGTG 
TTGAGAAGTG AAATATGGCA TCCGCAGCGT GGCTCCGAAA TTCTTCCGTC TTTGATATTA 
AGCTACCATC ATCTTTCTTT ACCTCTGAAG CATTGTTTTG CTTATTGTTC TATTTTTCCC 
CAGGACCACC AATTCAACAA AGAGAAGCTG ATTTTATTAT GGATGGCAGA AGGTCTTTTA 
CATCCACAAC AAAACGAAGG AAGGAGAATG GAAGAGATCG GTGAGTCATA TTTTGATGAG 
CTTCTTGCCA AGTCTTTCTT TCAAAAATCT ATTGGAAGAA AAGGATCATG CTTTGTAATG 
CATGATCTAA TACATGAATT GGCTCAACAT GTGTCCGGAG ATTTTTGTGC TCGAGTGGAA 
GATGATGATA AATTACCAAA AGTATCTGAG AAGGCTCACC ACTTTTTGTA CTTTAATAGT 
GATTATAGCT ATTTGGTAGC ATTTAAGAAT TTTGAGGCTA TGACTAAAGC TAAATCTCTT 
CGAACATTCT TAGGAGTGAA GCCAACGGAA CACTATCCTT CTTATACTTT GAGTAAAAGA 
GTTTTGCAAG ATATATTACC AAAAATGTGG TGTCTACGCG TGTTATCATT ATGTGCATAT 
GAGATAACTG ATTTGCCTAA ATCGATAGGT AATTTGAAAC ATTTGCGTTA CTTGGATTTG 
TCTTTCACGA GGATTAAAAA ATTACCCGAA TCAGTATGTT GTTTATGCAA TTTACAAACG 
ATGATGCTGG GAGGATGTTC TAGACTTGAT GAATTGCCTT CAAAGATGGG AAAATTGATT 
TATTTGCGCT ATCTTGATAT TGATGGATGT AATTCATTGA GAGAAATGTC AAGTCATGGA 
ATTGATCGAT TAAAAAATTT ACAAAGGTTG ACTCAATTTA ATGTGGGCCA AAACAATGGA 
TTAAGAATTG GAGAATTGGG GGAGCTTTCA GAGATTCGAG GAAAACTTCA TATTTCAAAC 
ATGGAGAATG TGGTGAGTGT TGATGATGCA TCAAGGGCTA ATATGAAGGA TAAAAGTTAT 
CTTGATGAGT TAATCTTTGA TTGGTGTACC AGTGGTGTTA CACAAAGTGG TGCAACAACG 
CATGATATAC TCAACAAGTT ACAACCTCAT CCAAATTTAA AACAACTCTC CATCAAACAC 
TATCCTGGTG AAGGATTTCC AAATTGGCTT GGAGATCCTT CAGTGTTGAA TCTCGTGTCC 
CTTGAGCTTC GGGGTTGTGG CAATTGCTCA ACATTGCCAC CACTTGGACA GCTAACCCAG 
CTTAAATATC TGCAAATCTC AGGGATGAAT GGAGTAGAGT GTGTGGGTGA TGAGTTCTAT 
GGGAATGCTT CCTTTCAATT CCTAGAAACA CTATCATTTG AGGATATGCA GAACTGGGAG 
AAATGGTTAT GTTGTGGAGA ATTCCCTCGT CTCCAAAAGC TTTTTATACG AAGGTGTCCC 
AAACTCACTG GGAAATTACC AGAACAGCTT CTTTCATTAG TGGAACTTCA AATTCATGAA 
TGTCCGCAAC TGCTTATGGC TTCACTCACA GTTCCTATAA TTCGTCAGTT GAGGATGGTG 
GATTTCGGTA AACTGCAGTT GCAAATGCCG GGTTGTGACT TCACAGCTCT TCAAACTTCA 
GAAATTGAAA TTTTAGATGT GTCTCAGTGG AGTCAACTTC CAATGGCACC ACACCAGCTC 
TCAATTAGAG AATGTGATAA TGCGGAGTCT CTGCTAGAGG AGGAAATCTC GCAAACCAAC 
ATACATGATT GCAGTTTTTC TAGATCTCTG CACAAAGTTG GTTTACCCAC TACACTGAAA 
TCACTATTCA TCTCTGAGTG CTCGAAATTA GAGATTCTCG TACCTGAGTT GTCCAGATGC 
CATCTCCCAG TCCTTGAAAG TCTAGAAATC AAAGGTGGTG TTATCGATGA TTCTCTCACG 
TTATCCTTCT CATTAGGCAT CTTCCCCAAG TTGACTGATT TCACAATCGA TGGTCTTAAG 
GGGCTTGAGA AGCTCTCCAT TTTGGTTTCA GAGGGTGATC CCACATCTTT ATGTTCCCTG 
CGTCTAATTG GTTGCTCTGA TCTTGAATCT ATCGAATTGC ACGCTCTCAA CTTGGAGTCT 
TGTTTGATTG ATAGGTGCTT CAACCTTAGG TCGTTGGCAC ACACACACTC ATATGTACAG 
GAGTTGAAAT TATGGGCTTG TCCAGAATTG TTGTTTCAGA GAGAGGGTTT GCCTTCCAAC 
CTACGTAAAC TTGAAATTGG CGAGTGCAAC CAACTCACGC CCCAGGTGGA GTGGGGTTTG 
CAAAGGCTGA CCTCTCTTAC TCATTTCACA ATCACCGGTG GATGTGAAGA CATTGAATTA 
TTTCCCAAGG AGTGTTTGCT GCCCTCTTCT CTGACTTCTC TTCAAATAGA AATGCTTCCC 
AATCTCAAGT CTCTTGACAG CGGGGGTCTT CAACAGCTCA CCTCTCTAAA ACGATTAGAT 
ATTTATGGAT GCTCGAGGCT CCAATCATTG ACAGAAGCGG GTCTTCAACA CCTCACCTCT 
CTTGAAACGT TGTGGATCGC ACACTGCCCA GTGCTCCAAT CATTGACAGA AGCGGGTCTT 
CAACACCTCA CCTCTCTTGA AACGTTGTGG ATCCTAGACT GCCCAGTGCT CCAATCATTG 
ACAGAAGCGG GTATTCAACA CCTCACCTCT CTTGAAACGT TAGTGATCGA AAACTGCCCA 
ATGCTGCAAT CCTTGACAAA AGTGGGTCTT CAACACCTCA CCTCTCTTAA AGCATTGTAT 
ATCAAAAACT GTTGTAAGCT CAAATACTTG ACAAAAGAAA GACTTCCAGA CTCCCTCTCT 
CTTCTGAGGA TCTCCGAGTG TCCTTTACTG GAAAAACGGT GTCAATTTGA GAAAGGGAAA 
GAATGGCGTT ATATAGCTCA CGTTCCAAAA ATAATGATCA ATGGTGTGCT ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073256.1
CDS	16..4812
Translation	MLTSFPSKVCLHQLKLLSHHQGFNPSIPRVPTFHKLNHFSQFHHYHRHLRFCSINGHAGASGMSNGHDYSANHEDSSAKGKNGEGELHTSKDLLKKLKRYGISGILSYGLLNTAYYLTTFLLVWFYIAPAPGRMGYLAAVERFVKIMAMVWAGSQVTKLVRAGGALALAPIVDRGLSWFTLKFKFESQGKFGGIAVTDTNPLNSYCKATVDTDMADVLLSASLQVLFERLASPELINFIRRRNLSDELLSELKRKLVVVLNVLDDAEVKQFSNPNVKEWLVHVKGAVYDAEDLLDEIATDALRCKMEAADSQTGGTLKAWKWNKFSASVKTPFAIKSMESRVRGMIDLLEKIALEKVGLGLAEGGGEKRSPRPRSPISTSLEDDSIVVGRDEIQKEMVEWLLSDNTTGDKMGVMSIVGMGGSGKTTLARRLYNDEEVKKHFDLQAWVCVSTEFLLIKLTKTILEEIRSPPTSADNLNLLQLQLKEQLSNKKFLLVLDDVWNLNPRDEGYMELSDREGWERLRTPLLAAAEGSKIVVTSRNKSVAEAMKAAPTHDLGKLSSEDSWSLFKKHAFGDRDPNAFLELERIGRQIVDKCQGLPLAVKALGCLLYSKDEKMEWDDVLRSEIWHPQRGSEILPSLILSYHHLSLPLKHCFAYCSIFPQDHQFNKEKLILLWMAEGLLHPQQNEGRRMEEIGESYFDELLAKSFFQKSIGRKGSCFVMHDLIHELAQHVSGDFCARVEDDDKLPKVSEKAHHFLYFNSDYSYLVAFKNFEAMTKAKSLRTFLGVKPTEHYPSYTLSKRVLQDILPKMWCLRVLSLCAYEITDLPKSIGNLKHLRYLDLSFTRIKKLPESVCCLCNLQTMMLGGCSRLDELPSKMGKLIYLRYLDIDGCNSLREMSSHGIDRLKNLQRLTQFNVGQNNGLRIGELGELSEIRGKLHISNMENVVSVDDASRANMKDKSYLDELIFDWCTSGVTQSGATTHDILNKLQPHPNLKQLSIKHYPGEGFPNWLGDPSVLNLVSLELRGCGNCSTLPPLGQLTQLKYLQISGMNGVECVGDEFYGNASFQFLETLSFEDMQNWEKWLCCGEFPRLQKLFIRRCPKLTGKLPEQLLSLVELQIHECPQLLMASLTVPIIRQLRMVDFGKLQLQMPGCDFTALQTSEIEILDVSQWSQLPMAPHQLSIRECDNAESLLEEEISQTNIHDCSFSRSLHKVGLPTTLKSLFISECSKLEILVPELSRCHLPVLESLEIKGGVIDDSLTLSFSLGIFPKLTDFTIDGLKGLEKLSILVSEGDPTSLCSLRLIGCSDLESIELHALNLESCLIDRCFNLRSLAHTHSYVQELKLWACPELLFQREGLPSNLRKLEIGECNQLTPQVEWGLQRLTSLTHFTITGGCEDIELFPKECLLPSSLTSLQIEMLPNLKSLDSGGLQQLTSLKRLDIYGCSRLQSLTEAGLQHLTSLETLWIAHCPVLQSLTEAGLQHLTSLETLWILDCPVLQSLTEAGIQHLTSLETLVIENCPMLQSLTKVGLQHLTSLKALYIKNCCKLKYLTKERLPDSLSLLRISECPLLEKRCQFEKGKEWRYIAHVPKIMINGVLY

Target ORF information:

RefSeq Version	XM_019217711.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217711.1

ORF Insert Sequence:

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGATCTTC TAAAGAAACT GAAGAGATAT 
GGAATTTCTG GAATATTATC CTATGGACTC CTGAATACTG CATACTATCT TACAACGTTT 
CTCTTGGTAT GGTTTTATAT TGCTCCAGCA CCAGGAAGGA TGGGTTATTT GGCGGCAGTT 
GAAAGATTTG TCAAAATCAT GGCTATGGTG TGGGCAGGCA GCCAGGTCAC TAAACTTGTC 
AGAGCTGGAG GGGCCCTTGC ACTTGCACCT ATTGTAGATA GAGGATTGTC ATGGTTTACA 
TTAAAATTCA AGTTTGAGTC TCAGGGAAAG TTTGGTGGTA TCGCGGTAAC AGACACCAAT 
CCTCTGAATT CCTATTGCAA AGCAACAGTC GACACAGATA TGGCGGACGT CCTCCTCTCA 
GCTTCGCTTC AAGTTCTATT CGAAAGGTTG GCTTCTCCAG AGCTCATAAA CTTCATTCGG 
CGACGGAACC TTAGCGATGA ACTCCTCAGC GAGTTGAAGA GGAAACTCGT GGTTGTTCTC 
AATGTGCTCG ATGATGCGGA GGTGAAGCAA TTTTCCAACC CAAATGTCAA AGAGTGGCTC 
GTCCATGTCA AGGGTGCTGT GTATGATGCG GAAGACCTGT TGGACGAGAT CGCTACCGAC 
GCTTTGCGTT GCAAGATGGA AGCTGCTGAC TCCCAAACCG GCGGAACTCT TAAGGCGTGG 
AAATGGAACA AGTTCTCTGC TTCTGTGAAG ACTCCATTTG CTATCAAAAG CATGGAGTCC 
AGGGTCAGGG GCATGATTGA TCTACTGGAA AAAATTGCAC TAGAAAAAGT TGGGTTGGGG 
CTGGCAGAGG GTGGGGGCGA GAAACGGTCA CCAAGACCAA GATCACCAAT ATCCACTTCT 
TTGGAGGATG ACTCCATTGT TGTCGGCAGG GATGAAATTC AGAAGGAGAT GGTGGAGTGG 
TTGCTTTCTG ACAATACAAC AGGCGACAAA ATGGGGGTGA TGTCCATAGT GGGCATGGGC 
GGCAGCGGCA AGACCACGCT TGCTCGGCGT CTCTATAACG ATGAGGAAGT GAAGAAACAC 
TTCGACTTGC AAGCATGGGT CTGTGTTTCC ACTGAGTTTC TCCTTATCAA ACTCACCAAA 
ACAATTCTTG AGGAAATTCG TTCTCCACCT ACTTCCGCTG ACAACCTAAA TTTGCTTCAG 
CTTCAACTCA AAGAGCAACT TAGTAACAAG AAATTTCTGC TTGTTCTTGA TGACGTCTGG 
AATTTGAATC CTCGTGATGA AGGTTATATG GAGCTTAGTG ATCGTGAAGG TTGGGAACGT 
CTACGAACTC CACTCCTCGC TGCAGCAGAG GGAAGCAAGA TTGTTGTGAC CAGTCGTAAT 
AAATCTGTTG CAGAAGCCAT GAAAGCGGCC CCTACTCATG ATCTTGGGAA ATTAAGCTCT 
GAAGATAGTT GGTCCCTGTT TAAAAAGCAT GCATTTGGAG ATAGAGACCC GAACGCATTC 
CTTGAGCTTG AACGCATAGG CAGACAGATT GTGGACAAGT GTCAAGGATT GCCTTTGGCT 
GTGAAAGCAC TCGGCTGTCT CTTGTATTCT AAGGACGAAA AAATGGAATG GGACGATGTG 
TTGAGAAGTG AAATATGGCA TCCGCAGCGT GGCTCCGAAA TTCTTCCGTC TTTGATATTA 
AGCTACCATC ATCTTTCTTT ACCTCTGAAG CATTGTTTTG CTTATTGTTC TATTTTTCCC 
CAGGACCACC AATTCAACAA AGAGAAGCTG ATTTTATTAT GGATGGCAGA AGGTCTTTTA 
CATCCACAAC AAAACGAAGG AAGGAGAATG GAAGAGATCG GTGAGTCATA TTTTGATGAG 
CTTCTTGCCA AGTCTTTCTT TCAAAAATCT ATTGGAAGAA AAGGATCATG CTTTGTAATG 
CATGATCTAA TACATGAATT GGCTCAACAT GTGTCCGGAG ATTTTTGTGC TCGAGTGGAA 
GATGATGATA AATTACCAAA AGTATCTGAG AAGGCTCACC ACTTTTTGTA CTTTAATAGT 
GATTATAGCT ATTTGGTAGC ATTTAAGAAT TTTGAGGCTA TGACTAAAGC TAAATCTCTT 
CGAACATTCT TAGGAGTGAA GCCAACGGAA CACTATCCTT CTTATACTTT GAGTAAAAGA 
GTTTTGCAAG ATATATTACC AAAAATGTGG TGTCTACGCG TGTTATCATT ATGTGCATAT 
GAGATAACTG ATTTGCCTAA ATCGATAGGT AATTTGAAAC ATTTGCGTTA CTTGGATTTG 
TCTTTCACGA GGATTAAAAA ATTACCCGAA TCAGTATGTT GTTTATGCAA TTTACAAACG 
ATGATGCTGG GAGGATGTTC TAGACTTGAT GAATTGCCTT CAAAGATGGG AAAATTGATT 
TATTTGCGCT ATCTTGATAT TGATGGATGT AATTCATTGA GAGAAATGTC AAGTCATGGA 
ATTGATCGAT TAAAAAATTT ACAAAGGTTG ACTCAATTTA ATGTGGGCCA AAACAATGGA 
TTAAGAATTG GAGAATTGGG GGAGCTTTCA GAGATTCGAG GAAAACTTCA TATTTCAAAC 
ATGGAGAATG TGGTGAGTGT TGATGATGCA TCAAGGGCTA ATATGAAGGA TAAAAGTTAT 
CTTGATGAGT TAATCTTTGA TTGGTGTACC AGTGGTGTTA CACAAAGTGG TGCAACAACG 
CATGATATAC TCAACAAGTT ACAACCTCAT CCAAATTTAA AACAACTCTC CATCAAACAC 
TATCCTGGTG AAGGATTTCC AAATTGGCTT GGAGATCCTT CAGTGTTGAA TCTCGTGTCC 
CTTGAGCTTC GGGGTTGTGG CAATTGCTCA ACATTGCCAC CACTTGGACA GCTAACCCAG 
CTTAAATATC TGCAAATCTC AGGGATGAAT GGAGTAGAGT GTGTGGGTGA TGAGTTCTAT 
GGGAATGCTT CCTTTCAATT CCTAGAAACA CTATCATTTG AGGATATGCA GAACTGGGAG 
AAATGGTTAT GTTGTGGAGA ATTCCCTCGT CTCCAAAAGC TTTTTATACG AAGGTGTCCC 
AAACTCACTG GGAAATTACC AGAACAGCTT CTTTCATTAG TGGAACTTCA AATTCATGAA 
TGTCCGCAAC TGCTTATGGC TTCACTCACA GTTCCTATAA TTCGTCAGTT GAGGATGGTG 
GATTTCGGTA AACTGCAGTT GCAAATGCCG GGTTGTGACT TCACAGCTCT TCAAACTTCA 
GAAATTGAAA TTTTAGATGT GTCTCAGTGG AGTCAACTTC CAATGGCACC ACACCAGCTC 
TCAATTAGAG AATGTGATAA TGCGGAGTCT CTGCTAGAGG AGGAAATCTC GCAAACCAAC 
ATACATGATT GCAGTTTTTC TAGATCTCTG CACAAAGTTG GTTTACCCAC TACACTGAAA 
TCACTATTCA TCTCTGAGTG CTCGAAATTA GAGATTCTCG TACCTGAGTT GTCCAGATGC 
CATCTCCCAG TCCTTGAAAG TCTAGAAATC AAAGGTGGTG TTATCGATGA TTCTCTCACG 
TTATCCTTCT CATTAGGCAT CTTCCCCAAG TTGACTGATT TCACAATCGA TGGTCTTAAG 
GGGCTTGAGA AGCTCTCCAT TTTGGTTTCA GAGGGTGATC CCACATCTTT ATGTTCCCTG 
CGTCTAATTG GTTGCTCTGA TCTTGAATCT ATCGAATTGC ACGCTCTCAA CTTGGAGTCT 
TGTTTGATTG ATAGGTGCTT CAACCTTAGG TCGTTGGCAC ACACACACTC ATATGTACAG 
GAGTTGAAAT TATGGGCTTG TCCAGAATTG TTGTTTCAGA GAGAGGGTTT GCCTTCCAAC 
CTACGTAAAC TTGAAATTGG CGAGTGCAAC CAACTCACGC CCCAGGTGGA GTGGGGTTTG 
CAAAGGCTGA CCTCTCTTAC TCATTTCACA ATCACCGGTG GATGTGAAGA CATTGAATTA 
TTTCCCAAGG AGTGTTTGCT GCCCTCTTCT CTGACTTCTC TTCAAATAGA AATGCTTCCC 
AATCTCAAGT CTCTTGACAG CGGGGGTCTT CAACAGCTCA CCTCTCTAAA ACGATTAGAT 
ATTTATGGAT GCTCGAGGCT CCAATCATTG ACAGAAGCGG GTCTTCAACA CCTCACCTCT 
CTTGAAACGT TGTGGATCGC ACACTGCCCA GTGCTCCAAT CATTGACAGA AGCGGGTCTT 
CAACACCTCA CCTCTCTTGA AACGTTGTGG ATCCTAGACT GCCCAGTGCT CCAATCATTG 
ACAGAAGCGG GTATTCAACA CCTCACCTCT CTTGAAACGT TAGTGATCGA AAACTGCCCA 
ATGCTGCAAT CCTTGACAAA AGTGGGTCTT CAACACCTCA CCTCTCTTAA AGCATTGTAT 
ATCAAAAACT GTTGTAAGCT CAAATACTTG ACAAAAGAAA GACTTCCAGA CTCCCTCTCT 
CTTCTGAGGA TCTCCGAGTG TCCTTTACTG GAAAAACGGT GTCAATTTGA GAAAGGGAAA 
GAATGGCGTT ATATAGCTCA CGTTCCAAAA ATAATGATCA ATGGTGTGCT ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100853412 ( XM_019217712.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073257.1 Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa39243
Clone ID Related Accession (Same CDS sequence)	XM_019217712.1
Accession Version	XM_019217712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative disease resistance RPP13-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGAGTTTT ATATTGCTCC AGCACCAGGA 
AGGATGGGTT ATTTGGCGGC AGTTGAAAGA TTTGTCAAAA TCATGGCTAT GGTGTGGGCA 
GGCAGCCAGG TCACTAAACT TGTCAGAGCT GGAGGGGCCC TTGCACTTGC ACCTATTGTA 
GATAGAGGAT TGTCATGGTT TACATTAAAA TTCAAGTTTG AGTCTCAGGG AAAGTTTGGT 
GGTATCGCGG TAACAGACAC CAATCCTCTG AATTCCTATT GCAAAGCAAC AGTCGACACA 
GATATGGCGG ACGTCCTCCT CTCAGCTTCG CTTCAAGTTC TATTCGAAAG GTTGGCTTCT 
CCAGAGCTCA TAAACTTCAT TCGGCGACGG AACCTTAGCG ATGAACTCCT CAGCGAGTTG 
AAGAGGAAAC TCGTGGTTGT TCTCAATGTG CTCGATGATG CGGAGGTGAA GCAATTTTCC 
AACCCAAATG TCAAAGAGTG GCTCGTCCAT GTCAAGGGTG CTGTGTATGA TGCGGAAGAC 
CTGTTGGACG AGATCGCTAC CGACGCTTTG CGTTGCAAGA TGGAAGCTGC TGACTCCCAA 
ACCGGCGGAA CTCTTAAGGC GTGGAAATGG AACAAGTTCT CTGCTTCTGT GAAGACTCCA 
TTTGCTATCA AAAGCATGGA GTCCAGGGTC AGGGGCATGA TTGATCTACT GGAAAAAATT 
GCACTAGAAA AAGTTGGGTT GGGGCTGGCA GAGGGTGGGG GCGAGAAACG GTCACCAAGA 
CCAAGATCAC CAATATCCAC TTCTTTGGAG GATGACTCCA TTGTTGTCGG CAGGGATGAA 
ATTCAGAAGG AGATGGTGGA GTGGTTGCTT TCTGACAATA CAACAGGCGA CAAAATGGGG 
GTGATGTCCA TAGTGGGCAT GGGCGGCAGC GGCAAGACCA CGCTTGCTCG GCGTCTCTAT 
AACGATGAGG AAGTGAAGAA ACACTTCGAC TTGCAAGCAT GGGTCTGTGT TTCCACTGAG 
TTTCTCCTTA TCAAACTCAC CAAAACAATT CTTGAGGAAA TTCGTTCTCC ACCTACTTCC 
GCTGACAACC TAAATTTGCT TCAGCTTCAA CTCAAAGAGC AACTTAGTAA CAAGAAATTT 
CTGCTTGTTC TTGATGACGT CTGGAATTTG AATCCTCGTG ATGAAGGTTA TATGGAGCTT 
AGTGATCGTG AAGGTTGGGA ACGTCTACGA ACTCCACTCC TCGCTGCAGC AGAGGGAAGC 
AAGATTGTTG TGACCAGTCG TAATAAATCT GTTGCAGAAG CCATGAAAGC GGCCCCTACT 
CATGATCTTG GGAAATTAAG CTCTGAAGAT AGTTGGTCCC TGTTTAAAAA GCATGCATTT 
GGAGATAGAG ACCCGAACGC ATTCCTTGAG CTTGAACGCA TAGGCAGACA GATTGTGGAC 
AAGTGTCAAG GATTGCCTTT GGCTGTGAAA GCACTCGGCT GTCTCTTGTA TTCTAAGGAC 
GAAAAAATGG AATGGGACGA TGTGTTGAGA AGTGAAATAT GGCATCCGCA GCGTGGCTCC 
GAAATTCTTC CGTCTTTGAT ATTAAGCTAC CATCATCTTT CTTTACCTCT GAAGCATTGT 
TTTGCTTATT GTTCTATTTT TCCCCAGGAC CACCAATTCA ACAAAGAGAA GCTGATTTTA 
TTATGGATGG CAGAAGGTCT TTTACATCCA CAACAAAACG AAGGAAGGAG AATGGAAGAG 
ATCGGTGAGT CATATTTTGA TGAGCTTCTT GCCAAGTCTT TCTTTCAAAA ATCTATTGGA 
AGAAAAGGAT CATGCTTTGT AATGCATGAT CTAATACATG AATTGGCTCA ACATGTGTCC 
GGAGATTTTT GTGCTCGAGT GGAAGATGAT GATAAATTAC CAAAAGTATC TGAGAAGGCT 
CACCACTTTT TGTACTTTAA TAGTGATTAT AGCTATTTGG TAGCATTTAA GAATTTTGAG 
GCTATGACTA AAGCTAAATC TCTTCGAACA TTCTTAGGAG TGAAGCCAAC GGAACACTAT 
CCTTCTTATA CTTTGAGTAA AAGAGTTTTG CAAGATATAT TACCAAAAAT GTGGTGTCTA 
CGCGTGTTAT CATTATGTGC ATATGAGATA ACTGATTTGC CTAAATCGAT AGGTAATTTG 
AAACATTTGC GTTACTTGGA TTTGTCTTTC ACGAGGATTA AAAAATTACC CGAATCAGTA 
TGTTGTTTAT GCAATTTACA AACGATGATG CTGGGAGGAT GTTCTAGACT TGATGAATTG 
CCTTCAAAGA TGGGAAAATT GATTTATTTG CGCTATCTTG ATATTGATGG ATGTAATTCA 
TTGAGAGAAA TGTCAAGTCA TGGAATTGAT CGATTAAAAA ATTTACAAAG GTTGACTCAA 
TTTAATGTGG GCCAAAACAA TGGATTAAGA ATTGGAGAAT TGGGGGAGCT TTCAGAGATT 
CGAGGAAAAC TTCATATTTC AAACATGGAG AATGTGGTGA GTGTTGATGA TGCATCAAGG 
GCTAATATGA AGGATAAAAG TTATCTTGAT GAGTTAATCT TTGATTGGTG TACCAGTGGT 
GTTACACAAA GTGGTGCAAC AACGCATGAT ATACTCAACA AGTTACAACC TCATCCAAAT 
TTAAAACAAC TCTCCATCAA ACACTATCCT GGTGAAGGAT TTCCAAATTG GCTTGGAGAT 
CCTTCAGTGT TGAATCTCGT GTCCCTTGAG CTTCGGGGTT GTGGCAATTG CTCAACATTG 
CCACCACTTG GACAGCTAAC CCAGCTTAAA TATCTGCAAA TCTCAGGGAT GAATGGAGTA 
GAGTGTGTGG GTGATGAGTT CTATGGGAAT GCTTCCTTTC AATTCCTAGA AACACTATCA 
TTTGAGGATA TGCAGAACTG GGAGAAATGG TTATGTTGTG GAGAATTCCC TCGTCTCCAA 
AAGCTTTTTA TACGAAGGTG TCCCAAACTC ACTGGGAAAT TACCAGAACA GCTTCTTTCA 
TTAGTGGAAC TTCAAATTCA TGAATGTCCG CAACTGCTTA TGGCTTCACT CACAGTTCCT 
ATAATTCGTC AGTTGAGGAT GGTGGATTTC GGTAAACTGC AGTTGCAAAT GCCGGGTTGT 
GACTTCACAG CTCTTCAAAC TTCAGAAATT GAAATTTTAG ATGTGTCTCA GTGGAGTCAA 
CTTCCAATGG CACCACACCA GCTCTCAATT AGAGAATGTG ATAATGCGGA GTCTCTGCTA 
GAGGAGGAAA TCTCGCAAAC CAACATACAT GATTGCAGTT TTTCTAGATC TCTGCACAAA 
GTTGGTTTAC CCACTACACT GAAATCACTA TTCATCTCTG AGTGCTCGAA ATTAGAGATT 
CTCGTACCTG AGTTGTCCAG ATGCCATCTC CCAGTCCTTG AAAGTCTAGA AATCAAAGGT 
GGTGTTATCG ATGATTCTCT CACGTTATCC TTCTCATTAG GCATCTTCCC CAAGTTGACT 
GATTTCACAA TCGATGGTCT TAAGGGGCTT GAGAAGCTCT CCATTTTGGT TTCAGAGGGT 
GATCCCACAT CTTTATGTTC CCTGCGTCTA ATTGGTTGCT CTGATCTTGA ATCTATCGAA 
TTGCACGCTC TCAACTTGGA GTCTTGTTTG ATTGATAGGT GCTTCAACCT TAGGTCGTTG 
GCACACACAC ACTCATATGT ACAGGAGTTG AAATTATGGG CTTGTCCAGA ATTGTTGTTT 
CAGAGAGAGG GTTTGCCTTC CAACCTACGT AAACTTGAAA TTGGCGAGTG CAACCAACTC 
ACGCCCCAGG TGGAGTGGGG TTTGCAAAGG CTGACCTCTC TTACTCATTT CACAATCACC 
GGTGGATGTG AAGACATTGA ATTATTTCCC AAGGAGTGTT TGCTGCCCTC TTCTCTGACT 
TCTCTTCAAA TAGAAATGCT TCCCAATCTC AAGTCTCTTG ACAGCGGGGG TCTTCAACAG 
CTCACCTCTC TAAAACGATT AGATATTTAT GGATGCTCGA GGCTCCAATC ATTGACAGAA 
GCGGGTCTTC AACACCTCAC CTCTCTTGAA ACGTTGTGGA TCGCACACTG CCCAGTGCTC 
CAATCATTGA CAGAAGCGGG TCTTCAACAC CTCACCTCTC TTGAAACGTT GTGGATCCTA 
GACTGCCCAG TGCTCCAATC ATTGACAGAA GCGGGTATTC AACACCTCAC CTCTCTTGAA 
ACGTTAGTGA TCGAAAACTG CCCAATGCTG CAATCCTTGA CAAAAGTGGG TCTTCAACAC 
CTCACCTCTC TTAAAGCATT GTATATCAAA AACTGTTGTA AGCTCAAATA CTTGACAAAA 
GAAAGACTTC CAGACTCCCT CTCTCTTCTG AGGATCTCCG AGTGTCCTTT ACTGGAAAAA 
CGGTGTCAAT TTGAGAAAGG GAAAGAATGG CGTTATATAG CTCACGTTCC AAAAATAATG 
ATCAATGGTG TGCTATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073257.1
CDS	16..4716
Translation	MLTSFPSKVCLHQLKLLSHHQGFNPSIPRVPTFHKLNHFSQFHHYHRHLRFCSINGHAGASGMSNGHDYSANHEDSSAKGKNGEGELHTSKEFYIAPAPGRMGYLAAVERFVKIMAMVWAGSQVTKLVRAGGALALAPIVDRGLSWFTLKFKFESQGKFGGIAVTDTNPLNSYCKATVDTDMADVLLSASLQVLFERLASPELINFIRRRNLSDELLSELKRKLVVVLNVLDDAEVKQFSNPNVKEWLVHVKGAVYDAEDLLDEIATDALRCKMEAADSQTGGTLKAWKWNKFSASVKTPFAIKSMESRVRGMIDLLEKIALEKVGLGLAEGGGEKRSPRPRSPISTSLEDDSIVVGRDEIQKEMVEWLLSDNTTGDKMGVMSIVGMGGSGKTTLARRLYNDEEVKKHFDLQAWVCVSTEFLLIKLTKTILEEIRSPPTSADNLNLLQLQLKEQLSNKKFLLVLDDVWNLNPRDEGYMELSDREGWERLRTPLLAAAEGSKIVVTSRNKSVAEAMKAAPTHDLGKLSSEDSWSLFKKHAFGDRDPNAFLELERIGRQIVDKCQGLPLAVKALGCLLYSKDEKMEWDDVLRSEIWHPQRGSEILPSLILSYHHLSLPLKHCFAYCSIFPQDHQFNKEKLILLWMAEGLLHPQQNEGRRMEEIGESYFDELLAKSFFQKSIGRKGSCFVMHDLIHELAQHVSGDFCARVEDDDKLPKVSEKAHHFLYFNSDYSYLVAFKNFEAMTKAKSLRTFLGVKPTEHYPSYTLSKRVLQDILPKMWCLRVLSLCAYEITDLPKSIGNLKHLRYLDLSFTRIKKLPESVCCLCNLQTMMLGGCSRLDELPSKMGKLIYLRYLDIDGCNSLREMSSHGIDRLKNLQRLTQFNVGQNNGLRIGELGELSEIRGKLHISNMENVVSVDDASRANMKDKSYLDELIFDWCTSGVTQSGATTHDILNKLQPHPNLKQLSIKHYPGEGFPNWLGDPSVLNLVSLELRGCGNCSTLPPLGQLTQLKYLQISGMNGVECVGDEFYGNASFQFLETLSFEDMQNWEKWLCCGEFPRLQKLFIRRCPKLTGKLPEQLLSLVELQIHECPQLLMASLTVPIIRQLRMVDFGKLQLQMPGCDFTALQTSEIEILDVSQWSQLPMAPHQLSIRECDNAESLLEEEISQTNIHDCSFSRSLHKVGLPTTLKSLFISECSKLEILVPELSRCHLPVLESLEIKGGVIDDSLTLSFSLGIFPKLTDFTIDGLKGLEKLSILVSEGDPTSLCSLRLIGCSDLESIELHALNLESCLIDRCFNLRSLAHTHSYVQELKLWACPELLFQREGLPSNLRKLEIGECNQLTPQVEWGLQRLTSLTHFTITGGCEDIELFPKECLLPSSLTSLQIEMLPNLKSLDSGGLQQLTSLKRLDIYGCSRLQSLTEAGLQHLTSLETLWIAHCPVLQSLTEAGLQHLTSLETLWILDCPVLQSLTEAGIQHLTSLETLVIENCPMLQSLTKVGLQHLTSLKALYIKNCCKLKYLTKERLPDSLSLLRISECPLLEKRCQFEKGKEWRYIAHVPKIMINGVLY

Target ORF information:

RefSeq Version	XM_019217712.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217712.1

ORF Insert Sequence:

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGAGTTTT ATATTGCTCC AGCACCAGGA 
AGGATGGGTT ATTTGGCGGC AGTTGAAAGA TTTGTCAAAA TCATGGCTAT GGTGTGGGCA 
GGCAGCCAGG TCACTAAACT TGTCAGAGCT GGAGGGGCCC TTGCACTTGC ACCTATTGTA 
GATAGAGGAT TGTCATGGTT TACATTAAAA TTCAAGTTTG AGTCTCAGGG AAAGTTTGGT 
GGTATCGCGG TAACAGACAC CAATCCTCTG AATTCCTATT GCAAAGCAAC AGTCGACACA 
GATATGGCGG ACGTCCTCCT CTCAGCTTCG CTTCAAGTTC TATTCGAAAG GTTGGCTTCT 
CCAGAGCTCA TAAACTTCAT TCGGCGACGG AACCTTAGCG ATGAACTCCT CAGCGAGTTG 
AAGAGGAAAC TCGTGGTTGT TCTCAATGTG CTCGATGATG CGGAGGTGAA GCAATTTTCC 
AACCCAAATG TCAAAGAGTG GCTCGTCCAT GTCAAGGGTG CTGTGTATGA TGCGGAAGAC 
CTGTTGGACG AGATCGCTAC CGACGCTTTG CGTTGCAAGA TGGAAGCTGC TGACTCCCAA 
ACCGGCGGAA CTCTTAAGGC GTGGAAATGG AACAAGTTCT CTGCTTCTGT GAAGACTCCA 
TTTGCTATCA AAAGCATGGA GTCCAGGGTC AGGGGCATGA TTGATCTACT GGAAAAAATT 
GCACTAGAAA AAGTTGGGTT GGGGCTGGCA GAGGGTGGGG GCGAGAAACG GTCACCAAGA 
CCAAGATCAC CAATATCCAC TTCTTTGGAG GATGACTCCA TTGTTGTCGG CAGGGATGAA 
ATTCAGAAGG AGATGGTGGA GTGGTTGCTT TCTGACAATA CAACAGGCGA CAAAATGGGG 
GTGATGTCCA TAGTGGGCAT GGGCGGCAGC GGCAAGACCA CGCTTGCTCG GCGTCTCTAT 
AACGATGAGG AAGTGAAGAA ACACTTCGAC TTGCAAGCAT GGGTCTGTGT TTCCACTGAG 
TTTCTCCTTA TCAAACTCAC CAAAACAATT CTTGAGGAAA TTCGTTCTCC ACCTACTTCC 
GCTGACAACC TAAATTTGCT TCAGCTTCAA CTCAAAGAGC AACTTAGTAA CAAGAAATTT 
CTGCTTGTTC TTGATGACGT CTGGAATTTG AATCCTCGTG ATGAAGGTTA TATGGAGCTT 
AGTGATCGTG AAGGTTGGGA ACGTCTACGA ACTCCACTCC TCGCTGCAGC AGAGGGAAGC 
AAGATTGTTG TGACCAGTCG TAATAAATCT GTTGCAGAAG CCATGAAAGC GGCCCCTACT 
CATGATCTTG GGAAATTAAG CTCTGAAGAT AGTTGGTCCC TGTTTAAAAA GCATGCATTT 
GGAGATAGAG ACCCGAACGC ATTCCTTGAG CTTGAACGCA TAGGCAGACA GATTGTGGAC 
AAGTGTCAAG GATTGCCTTT GGCTGTGAAA GCACTCGGCT GTCTCTTGTA TTCTAAGGAC 
GAAAAAATGG AATGGGACGA TGTGTTGAGA AGTGAAATAT GGCATCCGCA GCGTGGCTCC 
GAAATTCTTC CGTCTTTGAT ATTAAGCTAC CATCATCTTT CTTTACCTCT GAAGCATTGT 
TTTGCTTATT GTTCTATTTT TCCCCAGGAC CACCAATTCA ACAAAGAGAA GCTGATTTTA 
TTATGGATGG CAGAAGGTCT TTTACATCCA CAACAAAACG AAGGAAGGAG AATGGAAGAG 
ATCGGTGAGT CATATTTTGA TGAGCTTCTT GCCAAGTCTT TCTTTCAAAA ATCTATTGGA 
AGAAAAGGAT CATGCTTTGT AATGCATGAT CTAATACATG AATTGGCTCA ACATGTGTCC 
GGAGATTTTT GTGCTCGAGT GGAAGATGAT GATAAATTAC CAAAAGTATC TGAGAAGGCT 
CACCACTTTT TGTACTTTAA TAGTGATTAT AGCTATTTGG TAGCATTTAA GAATTTTGAG 
GCTATGACTA AAGCTAAATC TCTTCGAACA TTCTTAGGAG TGAAGCCAAC GGAACACTAT 
CCTTCTTATA CTTTGAGTAA AAGAGTTTTG CAAGATATAT TACCAAAAAT GTGGTGTCTA 
CGCGTGTTAT CATTATGTGC ATATGAGATA ACTGATTTGC CTAAATCGAT AGGTAATTTG 
AAACATTTGC GTTACTTGGA TTTGTCTTTC ACGAGGATTA AAAAATTACC CGAATCAGTA 
TGTTGTTTAT GCAATTTACA AACGATGATG CTGGGAGGAT GTTCTAGACT TGATGAATTG 
CCTTCAAAGA TGGGAAAATT GATTTATTTG CGCTATCTTG ATATTGATGG ATGTAATTCA 
TTGAGAGAAA TGTCAAGTCA TGGAATTGAT CGATTAAAAA ATTTACAAAG GTTGACTCAA 
TTTAATGTGG GCCAAAACAA TGGATTAAGA ATTGGAGAAT TGGGGGAGCT TTCAGAGATT 
CGAGGAAAAC TTCATATTTC AAACATGGAG AATGTGGTGA GTGTTGATGA TGCATCAAGG 
GCTAATATGA AGGATAAAAG TTATCTTGAT GAGTTAATCT TTGATTGGTG TACCAGTGGT 
GTTACACAAA GTGGTGCAAC AACGCATGAT ATACTCAACA AGTTACAACC TCATCCAAAT 
TTAAAACAAC TCTCCATCAA ACACTATCCT GGTGAAGGAT TTCCAAATTG GCTTGGAGAT 
CCTTCAGTGT TGAATCTCGT GTCCCTTGAG CTTCGGGGTT GTGGCAATTG CTCAACATTG 
CCACCACTTG GACAGCTAAC CCAGCTTAAA TATCTGCAAA TCTCAGGGAT GAATGGAGTA 
GAGTGTGTGG GTGATGAGTT CTATGGGAAT GCTTCCTTTC AATTCCTAGA AACACTATCA 
TTTGAGGATA TGCAGAACTG GGAGAAATGG TTATGTTGTG GAGAATTCCC TCGTCTCCAA 
AAGCTTTTTA TACGAAGGTG TCCCAAACTC ACTGGGAAAT TACCAGAACA GCTTCTTTCA 
TTAGTGGAAC TTCAAATTCA TGAATGTCCG CAACTGCTTA TGGCTTCACT CACAGTTCCT 
ATAATTCGTC AGTTGAGGAT GGTGGATTTC GGTAAACTGC AGTTGCAAAT GCCGGGTTGT 
GACTTCACAG CTCTTCAAAC TTCAGAAATT GAAATTTTAG ATGTGTCTCA GTGGAGTCAA 
CTTCCAATGG CACCACACCA GCTCTCAATT AGAGAATGTG ATAATGCGGA GTCTCTGCTA 
GAGGAGGAAA TCTCGCAAAC CAACATACAT GATTGCAGTT TTTCTAGATC TCTGCACAAA 
GTTGGTTTAC CCACTACACT GAAATCACTA TTCATCTCTG AGTGCTCGAA ATTAGAGATT 
CTCGTACCTG AGTTGTCCAG ATGCCATCTC CCAGTCCTTG AAAGTCTAGA AATCAAAGGT 
GGTGTTATCG ATGATTCTCT CACGTTATCC TTCTCATTAG GCATCTTCCC CAAGTTGACT 
GATTTCACAA TCGATGGTCT TAAGGGGCTT GAGAAGCTCT CCATTTTGGT TTCAGAGGGT 
GATCCCACAT CTTTATGTTC CCTGCGTCTA ATTGGTTGCT CTGATCTTGA ATCTATCGAA 
TTGCACGCTC TCAACTTGGA GTCTTGTTTG ATTGATAGGT GCTTCAACCT TAGGTCGTTG 
GCACACACAC ACTCATATGT ACAGGAGTTG AAATTATGGG CTTGTCCAGA ATTGTTGTTT 
CAGAGAGAGG GTTTGCCTTC CAACCTACGT AAACTTGAAA TTGGCGAGTG CAACCAACTC 
ACGCCCCAGG TGGAGTGGGG TTTGCAAAGG CTGACCTCTC TTACTCATTT CACAATCACC 
GGTGGATGTG AAGACATTGA ATTATTTCCC AAGGAGTGTT TGCTGCCCTC TTCTCTGACT 
TCTCTTCAAA TAGAAATGCT TCCCAATCTC AAGTCTCTTG ACAGCGGGGG TCTTCAACAG 
CTCACCTCTC TAAAACGATT AGATATTTAT GGATGCTCGA GGCTCCAATC ATTGACAGAA 
GCGGGTCTTC AACACCTCAC CTCTCTTGAA ACGTTGTGGA TCGCACACTG CCCAGTGCTC 
CAATCATTGA CAGAAGCGGG TCTTCAACAC CTCACCTCTC TTGAAACGTT GTGGATCCTA 
GACTGCCCAG TGCTCCAATC ATTGACAGAA GCGGGTATTC AACACCTCAC CTCTCTTGAA 
ACGTTAGTGA TCGAAAACTG CCCAATGCTG CAATCCTTGA CAAAAGTGGG TCTTCAACAC 
CTCACCTCTC TTAAAGCATT GTATATCAAA AACTGTTGTA AGCTCAAATA CTTGACAAAA 
GAAAGACTTC CAGACTCCCT CTCTCTTCTG AGGATCTCCG AGTGTCCTTT ACTGGAAAAA 
CGGTGTCAAT TTGAGAAAGG GAAAGAATGG CGTTATATAG CTCACGTTCC AAAAATAATG 
ATCAATGGTG TGCTATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100853412 ( XM_019217713.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073258.1 Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X3, mRNA.

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CloneID	OVa39244
Clone ID Related Accession (Same CDS sequence)	XM_019217713.1
Accession Version	XM_019217713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative disease resistance RPP13-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724179.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGATCTTC TAAAGAAACT GAAGAGATAT 
GGAATTTCTG GAATATTATC CTATGGACTC CTGAATACTG CATACTATCT TACAACGTTT 
CTCTTGGTAT GGTTTTATAT TGCTCCAGCA CCAGGAAGGA TGGGTTATTT GGCGGCAGTT 
GAAAGATTTG TCAAAATCAT GGCTATGGTG TGGGCAGGCA GCCAGGTCAC TAAACTTGTC 
AGAGCTGGAG GGGCCCTTGC ACTTGCACCT ATTGTAGATA GAGGATTGTC ATGGTTTACA 
TTAAAATTCA AGTTTGAGTC TCAGGGAAAG TTTGGTGGTA TCGCGGTAAC AGACACCAAT 
CCTCTGAATT CCTATTGCAA AGCAACAGTC GACACAGATA TGGCGGACGT CCTCCTCTCA 
GCTTCGCTTC AAGTTCTATT CGAAAGGTTG GCTTCTCCAG AGCTCATAAA CTTCATTCGG 
CGACGGAACC TTAGCGATGA ACTCCTCAGC GAGTTGAAGA GGAAACTCGT GGTTGTTCTC 
AATGTGCTCG ATGATGCGGA GGTGAAGCAA TTTTCCAACC CAAATGTCAA AGAGTGGCTC 
GTCCATGTCA AGGGTGCTGT GTATGATGCG GAAGACCTGT TGGACGAGAT CGCTACCGAC 
GCTTTGCGTT GCAAGATGGA AGCTGCTGAC TCCCAAACCG GCGGAACTCT TAAGGCGTGG 
AAATGGAACA AGTTCTCTGC TTCTGTGAAG ACTCCATTTG CTATCAAAAG CATGGAGTCC 
AGGGTCAGGG GCATGATTGA TCTACTGGAA AAAATTGCAC TAGAAAAAGT TGGGTTGGGG 
CTGGCAGAGG GTGGGGGCGA GAAACGGTCA CCAAGACCAA GATCACCAAT ATCCACTTCT 
TTGGAGGATG ACTCCATTGT TGTCGGCAGG GATGAAATTC AGAAGGAGAT GGTGGAGTGG 
TTGCTTTCTG ACAATACAAC AGGCGACAAA ATGGGGGTGA TGTCCATAGT GGGCATGGGC 
GGCAGCGGCA AGACCACGCT TGCTCGGCGT CTCTATAACG ATGAGGAAGT GAAGAAACAC 
TTCGACTTGC AAGCATGGCT TCAACTCAAA GAGCAACTTA GTAACAAGAA ATTTCTGCTT 
GTTCTTGATG ACGTCTGGAA TTTGAATCCT CGTGATGAAG GTTATATGGA GCTTAGTGAT 
CGTGAAGGTT GGGAACGTCT ACGAACTCCA CTCCTCGCTG CAGCAGAGGG AAGCAAGATT 
GTTGTGACCA GTCGTAATAA ATCTGTTGCA GAAGCCATGA AAGCGGCCCC TACTCATGAT 
CTTGGGAAAT TAAGCTCTGA AGATAGTTGG TCCCTGTTTA AAAAGCATGC ATTTGGAGAT 
AGAGACCCGA ACGCATTCCT TGAGCTTGAA CGCATAGGCA GACAGATTGT GGACAAGTGT 
CAAGGATTGC CTTTGGCTGT GAAAGCACTC GGCTGTCTCT TGTATTCTAA GGACGAAAAA 
ATGGAATGGG ACGATGTGTT GAGAAGTGAA ATATGGCATC CGCAGCGTGG CTCCGAAATT 
CTTCCGTCTT TGATATTAAG CTACCATCAT CTTTCTTTAC CTCTGAAGCA TTGTTTTGCT 
TATTGTTCTA TTTTTCCCCA GGACCACCAA TTCAACAAAG AGAAGCTGAT TTTATTATGG 
ATGGCAGAAG GTCTTTTACA TCCACAACAA AACGAAGGAA GGAGAATGGA AGAGATCGGT 
GAGTCATATT TTGATGAGCT TCTTGCCAAG TCTTTCTTTC AAAAATCTAT TGGAAGAAAA 
GGATCATGCT TTGTAATGCA TGATCTAATA CATGAATTGG CTCAACATGT GTCCGGAGAT 
TTTTGTGCTC GAGTGGAAGA TGATGATAAA TTACCAAAAG TATCTGAGAA GGCTCACCAC 
TTTTTGTACT TTAATAGTGA TTATAGCTAT TTGGTAGCAT TTAAGAATTT TGAGGCTATG 
ACTAAAGCTA AATCTCTTCG AACATTCTTA GGAGTGAAGC CAACGGAACA CTATCCTTCT 
TATACTTTGA GTAAAAGAGT TTTGCAAGAT ATATTACCAA AAATGTGGTG TCTACGCGTG 
TTATCATTAT GTGCATATGA GATAACTGAT TTGCCTAAAT CGATAGGTAA TTTGAAACAT 
TTGCGTTACT TGGATTTGTC TTTCACGAGG ATTAAAAAAT TACCCGAATC AGTATGTTGT 
TTATGCAATT TACAAACGAT GATGCTGGGA GGATGTTCTA GACTTGATGA ATTGCCTTCA 
AAGATGGGAA AATTGATTTA TTTGCGCTAT CTTGATATTG ATGGATGTAA TTCATTGAGA 
GAAATGTCAA GTCATGGAAT TGATCGATTA AAAAATTTAC AAAGGTTGAC TCAATTTAAT 
GTGGGCCAAA ACAATGGATT AAGAATTGGA GAATTGGGGG AGCTTTCAGA GATTCGAGGA 
AAACTTCATA TTTCAAACAT GGAGAATGTG GTGAGTGTTG ATGATGCATC AAGGGCTAAT 
ATGAAGGATA AAAGTTATCT TGATGAGTTA ATCTTTGATT GGTGTACCAG TGGTGTTACA 
CAAAGTGGTG CAACAACGCA TGATATACTC AACAAGTTAC AACCTCATCC AAATTTAAAA 
CAACTCTCCA TCAAACACTA TCCTGGTGAA GGATTTCCAA ATTGGCTTGG AGATCCTTCA 
GTGTTGAATC TCGTGTCCCT TGAGCTTCGG GGTTGTGGCA ATTGCTCAAC ATTGCCACCA 
CTTGGACAGC TAACCCAGCT TAAATATCTG CAAATCTCAG GGATGAATGG AGTAGAGTGT 
GTGGGTGATG AGTTCTATGG GAATGCTTCC TTTCAATTCC TAGAAACACT ATCATTTGAG 
GATATGCAGA ACTGGGAGAA ATGGTTATGT TGTGGAGAAT TCCCTCGTCT CCAAAAGCTT 
TTTATACGAA GGTGTCCCAA ACTCACTGGG AAATTACCAG AACAGCTTCT TTCATTAGTG 
GAACTTCAAA TTCATGAATG TCCGCAACTG CTTATGGCTT CACTCACAGT TCCTATAATT 
CGTCAGTTGA GGATGGTGGA TTTCGGTAAA CTGCAGTTGC AAATGCCGGG TTGTGACTTC 
ACAGCTCTTC AAACTTCAGA AATTGAAATT TTAGATGTGT CTCAGTGGAG TCAACTTCCA 
ATGGCACCAC ACCAGCTCTC AATTAGAGAA TGTGATAATG CGGAGTCTCT GCTAGAGGAG 
GAAATCTCGC AAACCAACAT ACATGATTGC AGTTTTTCTA GATCTCTGCA CAAAGTTGGT 
TTACCCACTA CACTGAAATC ACTATTCATC TCTGAGTGCT CGAAATTAGA GATTCTCGTA 
CCTGAGTTGT CCAGATGCCA TCTCCCAGTC CTTGAAAGTC TAGAAATCAA AGGTGGTGTT 
ATCGATGATT CTCTCACGTT ATCCTTCTCA TTAGGCATCT TCCCCAAGTT GACTGATTTC 
ACAATCGATG GTCTTAAGGG GCTTGAGAAG CTCTCCATTT TGGTTTCAGA GGGTGATCCC 
ACATCTTTAT GTTCCCTGCG TCTAATTGGT TGCTCTGATC TTGAATCTAT CGAATTGCAC 
GCTCTCAACT TGGAGTCTTG TTTGATTGAT AGGTGCTTCA ACCTTAGGTC GTTGGCACAC 
ACACACTCAT ATGTACAGGA GTTGAAATTA TGGGCTTGTC CAGAATTGTT GTTTCAGAGA 
GAGGGTTTGC CTTCCAACCT ACGTAAACTT GAAATTGGCG AGTGCAACCA ACTCACGCCC 
CAGGTGGAGT GGGGTTTGCA AAGGCTGACC TCTCTTACTC ATTTCACAAT CACCGGTGGA 
TGTGAAGACA TTGAATTATT TCCCAAGGAG TGTTTGCTGC CCTCTTCTCT GACTTCTCTT 
CAAATAGAAA TGCTTCCCAA TCTCAAGTCT CTTGACAGCG GGGGTCTTCA ACAGCTCACC 
TCTCTAAAAC GATTAGATAT TTATGGATGC TCGAGGCTCC AATCATTGAC AGAAGCGGGT 
CTTCAACACC TCACCTCTCT TGAAACGTTG TGGATCGCAC ACTGCCCAGT GCTCCAATCA 
TTGACAGAAG CGGGTCTTCA ACACCTCACC TCTCTTGAAA CGTTGTGGAT CCTAGACTGC 
CCAGTGCTCC AATCATTGAC AGAAGCGGGT ATTCAACACC TCACCTCTCT TGAAACGTTA 
GTGATCGAAA ACTGCCCAAT GCTGCAATCC TTGACAAAAG TGGGTCTTCA ACACCTCACC 
TCTCTTAAAG CATTGTATAT CAAAAACTGT TGTAAGCTCA AATACTTGAC AAAAGAAAGA 
CTTCCAGACT CCCTCTCTCT TCTGAGGATC TCCGAGTGTC CTTTACTGGA AAAACGGTGT 
CAATTTGAGA AAGGGAAAGA ATGGCGTTAT ATAGCTCACG TTCCAAAAAT AATGATCAAT 
GGTGTGCTAT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073258.1
CDS	16..4710
Translation	MLTSFPSKVCLHQLKLLSHHQGFNPSIPRVPTFHKLNHFSQFHHYHRHLRFCSINGHAGASGMSNGHDYSANHEDSSAKGKNGEGELHTSKDLLKKLKRYGISGILSYGLLNTAYYLTTFLLVWFYIAPAPGRMGYLAAVERFVKIMAMVWAGSQVTKLVRAGGALALAPIVDRGLSWFTLKFKFESQGKFGGIAVTDTNPLNSYCKATVDTDMADVLLSASLQVLFERLASPELINFIRRRNLSDELLSELKRKLVVVLNVLDDAEVKQFSNPNVKEWLVHVKGAVYDAEDLLDEIATDALRCKMEAADSQTGGTLKAWKWNKFSASVKTPFAIKSMESRVRGMIDLLEKIALEKVGLGLAEGGGEKRSPRPRSPISTSLEDDSIVVGRDEIQKEMVEWLLSDNTTGDKMGVMSIVGMGGSGKTTLARRLYNDEEVKKHFDLQAWLQLKEQLSNKKFLLVLDDVWNLNPRDEGYMELSDREGWERLRTPLLAAAEGSKIVVTSRNKSVAEAMKAAPTHDLGKLSSEDSWSLFKKHAFGDRDPNAFLELERIGRQIVDKCQGLPLAVKALGCLLYSKDEKMEWDDVLRSEIWHPQRGSEILPSLILSYHHLSLPLKHCFAYCSIFPQDHQFNKEKLILLWMAEGLLHPQQNEGRRMEEIGESYFDELLAKSFFQKSIGRKGSCFVMHDLIHELAQHVSGDFCARVEDDDKLPKVSEKAHHFLYFNSDYSYLVAFKNFEAMTKAKSLRTFLGVKPTEHYPSYTLSKRVLQDILPKMWCLRVLSLCAYEITDLPKSIGNLKHLRYLDLSFTRIKKLPESVCCLCNLQTMMLGGCSRLDELPSKMGKLIYLRYLDIDGCNSLREMSSHGIDRLKNLQRLTQFNVGQNNGLRIGELGELSEIRGKLHISNMENVVSVDDASRANMKDKSYLDELIFDWCTSGVTQSGATTHDILNKLQPHPNLKQLSIKHYPGEGFPNWLGDPSVLNLVSLELRGCGNCSTLPPLGQLTQLKYLQISGMNGVECVGDEFYGNASFQFLETLSFEDMQNWEKWLCCGEFPRLQKLFIRRCPKLTGKLPEQLLSLVELQIHECPQLLMASLTVPIIRQLRMVDFGKLQLQMPGCDFTALQTSEIEILDVSQWSQLPMAPHQLSIRECDNAESLLEEEISQTNIHDCSFSRSLHKVGLPTTLKSLFISECSKLEILVPELSRCHLPVLESLEIKGGVIDDSLTLSFSLGIFPKLTDFTIDGLKGLEKLSILVSEGDPTSLCSLRLIGCSDLESIELHALNLESCLIDRCFNLRSLAHTHSYVQELKLWACPELLFQREGLPSNLRKLEIGECNQLTPQVEWGLQRLTSLTHFTITGGCEDIELFPKECLLPSSLTSLQIEMLPNLKSLDSGGLQQLTSLKRLDIYGCSRLQSLTEAGLQHLTSLETLWIAHCPVLQSLTEAGLQHLTSLETLWILDCPVLQSLTEAGIQHLTSLETLVIENCPMLQSLTKVGLQHLTSLKALYIKNCCKLKYLTKERLPDSLSLLRISECPLLEKRCQFEKGKEWRYIAHVPKIMINGVLY

Target ORF information:

RefSeq Version	XM_019217713.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217713.1

ORF Insert Sequence:

ATGCTCACGT CTTTCCCGTC GAAAGTTTGC CTTCACCAGC TGAAGCTGCT TTCACATCAT 
CAGGGTTTCA ATCCCAGCAT TCCTAGGGTT CCCACATTCC ACAAACTCAA CCATTTCTCC 
CAATTCCACC ACTATCATCG TCATCTGCGT TTCTGCTCAA TCAATGGCCA TGCTGGCGCT 
TCTGGCATGT CAAATGGTCA TGACTACTCT GCCAACCATG AAGACTCAAG TGCAAAGGGC 
AAAAATGGTG AAGGAGAACT GCACACATCA AAAGATCTTC TAAAGAAACT GAAGAGATAT 
GGAATTTCTG GAATATTATC CTATGGACTC CTGAATACTG CATACTATCT TACAACGTTT 
CTCTTGGTAT GGTTTTATAT TGCTCCAGCA CCAGGAAGGA TGGGTTATTT GGCGGCAGTT 
GAAAGATTTG TCAAAATCAT GGCTATGGTG TGGGCAGGCA GCCAGGTCAC TAAACTTGTC 
AGAGCTGGAG GGGCCCTTGC ACTTGCACCT ATTGTAGATA GAGGATTGTC ATGGTTTACA 
TTAAAATTCA AGTTTGAGTC TCAGGGAAAG TTTGGTGGTA TCGCGGTAAC AGACACCAAT 
CCTCTGAATT CCTATTGCAA AGCAACAGTC GACACAGATA TGGCGGACGT CCTCCTCTCA 
GCTTCGCTTC AAGTTCTATT CGAAAGGTTG GCTTCTCCAG AGCTCATAAA CTTCATTCGG 
CGACGGAACC TTAGCGATGA ACTCCTCAGC GAGTTGAAGA GGAAACTCGT GGTTGTTCTC 
AATGTGCTCG ATGATGCGGA GGTGAAGCAA TTTTCCAACC CAAATGTCAA AGAGTGGCTC 
GTCCATGTCA AGGGTGCTGT GTATGATGCG GAAGACCTGT TGGACGAGAT CGCTACCGAC 
GCTTTGCGTT GCAAGATGGA AGCTGCTGAC TCCCAAACCG GCGGAACTCT TAAGGCGTGG 
AAATGGAACA AGTTCTCTGC TTCTGTGAAG ACTCCATTTG CTATCAAAAG CATGGAGTCC 
AGGGTCAGGG GCATGATTGA TCTACTGGAA AAAATTGCAC TAGAAAAAGT TGGGTTGGGG 
CTGGCAGAGG GTGGGGGCGA GAAACGGTCA CCAAGACCAA GATCACCAAT ATCCACTTCT 
TTGGAGGATG ACTCCATTGT TGTCGGCAGG GATGAAATTC AGAAGGAGAT GGTGGAGTGG 
TTGCTTTCTG ACAATACAAC AGGCGACAAA ATGGGGGTGA TGTCCATAGT GGGCATGGGC 
GGCAGCGGCA AGACCACGCT TGCTCGGCGT CTCTATAACG ATGAGGAAGT GAAGAAACAC 
TTCGACTTGC AAGCATGGCT TCAACTCAAA GAGCAACTTA GTAACAAGAA ATTTCTGCTT 
GTTCTTGATG ACGTCTGGAA TTTGAATCCT CGTGATGAAG GTTATATGGA GCTTAGTGAT 
CGTGAAGGTT GGGAACGTCT ACGAACTCCA CTCCTCGCTG CAGCAGAGGG AAGCAAGATT 
GTTGTGACCA GTCGTAATAA ATCTGTTGCA GAAGCCATGA AAGCGGCCCC TACTCATGAT 
CTTGGGAAAT TAAGCTCTGA AGATAGTTGG TCCCTGTTTA AAAAGCATGC ATTTGGAGAT 
AGAGACCCGA ACGCATTCCT TGAGCTTGAA CGCATAGGCA GACAGATTGT GGACAAGTGT 
CAAGGATTGC CTTTGGCTGT GAAAGCACTC GGCTGTCTCT TGTATTCTAA GGACGAAAAA 
ATGGAATGGG ACGATGTGTT GAGAAGTGAA ATATGGCATC CGCAGCGTGG CTCCGAAATT 
CTTCCGTCTT TGATATTAAG CTACCATCAT CTTTCTTTAC CTCTGAAGCA TTGTTTTGCT 
TATTGTTCTA TTTTTCCCCA GGACCACCAA TTCAACAAAG AGAAGCTGAT TTTATTATGG 
ATGGCAGAAG GTCTTTTACA TCCACAACAA AACGAAGGAA GGAGAATGGA AGAGATCGGT 
GAGTCATATT TTGATGAGCT TCTTGCCAAG TCTTTCTTTC AAAAATCTAT TGGAAGAAAA 
GGATCATGCT TTGTAATGCA TGATCTAATA CATGAATTGG CTCAACATGT GTCCGGAGAT 
TTTTGTGCTC GAGTGGAAGA TGATGATAAA TTACCAAAAG TATCTGAGAA GGCTCACCAC 
TTTTTGTACT TTAATAGTGA TTATAGCTAT TTGGTAGCAT TTAAGAATTT TGAGGCTATG 
ACTAAAGCTA AATCTCTTCG AACATTCTTA GGAGTGAAGC CAACGGAACA CTATCCTTCT 
TATACTTTGA GTAAAAGAGT TTTGCAAGAT ATATTACCAA AAATGTGGTG TCTACGCGTG 
TTATCATTAT GTGCATATGA GATAACTGAT TTGCCTAAAT CGATAGGTAA TTTGAAACAT 
TTGCGTTACT TGGATTTGTC TTTCACGAGG ATTAAAAAAT TACCCGAATC AGTATGTTGT 
TTATGCAATT TACAAACGAT GATGCTGGGA GGATGTTCTA GACTTGATGA ATTGCCTTCA 
AAGATGGGAA AATTGATTTA TTTGCGCTAT CTTGATATTG ATGGATGTAA TTCATTGAGA 
GAAATGTCAA GTCATGGAAT TGATCGATTA AAAAATTTAC AAAGGTTGAC TCAATTTAAT 
GTGGGCCAAA ACAATGGATT AAGAATTGGA GAATTGGGGG AGCTTTCAGA GATTCGAGGA 
AAACTTCATA TTTCAAACAT GGAGAATGTG GTGAGTGTTG ATGATGCATC AAGGGCTAAT 
ATGAAGGATA AAAGTTATCT TGATGAGTTA ATCTTTGATT GGTGTACCAG TGGTGTTACA 
CAAAGTGGTG CAACAACGCA TGATATACTC AACAAGTTAC AACCTCATCC AAATTTAAAA 
CAACTCTCCA TCAAACACTA TCCTGGTGAA GGATTTCCAA ATTGGCTTGG AGATCCTTCA 
GTGTTGAATC TCGTGTCCCT TGAGCTTCGG GGTTGTGGCA ATTGCTCAAC ATTGCCACCA 
CTTGGACAGC TAACCCAGCT TAAATATCTG CAAATCTCAG GGATGAATGG AGTAGAGTGT 
GTGGGTGATG AGTTCTATGG GAATGCTTCC TTTCAATTCC TAGAAACACT ATCATTTGAG 
GATATGCAGA ACTGGGAGAA ATGGTTATGT TGTGGAGAAT TCCCTCGTCT CCAAAAGCTT 
TTTATACGAA GGTGTCCCAA ACTCACTGGG AAATTACCAG AACAGCTTCT TTCATTAGTG 
GAACTTCAAA TTCATGAATG TCCGCAACTG CTTATGGCTT CACTCACAGT TCCTATAATT 
CGTCAGTTGA GGATGGTGGA TTTCGGTAAA CTGCAGTTGC AAATGCCGGG TTGTGACTTC 
ACAGCTCTTC AAACTTCAGA AATTGAAATT TTAGATGTGT CTCAGTGGAG TCAACTTCCA 
ATGGCACCAC ACCAGCTCTC AATTAGAGAA TGTGATAATG CGGAGTCTCT GCTAGAGGAG 
GAAATCTCGC AAACCAACAT ACATGATTGC AGTTTTTCTA GATCTCTGCA CAAAGTTGGT 
TTACCCACTA CACTGAAATC ACTATTCATC TCTGAGTGCT CGAAATTAGA GATTCTCGTA 
CCTGAGTTGT CCAGATGCCA TCTCCCAGTC CTTGAAAGTC TAGAAATCAA AGGTGGTGTT 
ATCGATGATT CTCTCACGTT ATCCTTCTCA TTAGGCATCT TCCCCAAGTT GACTGATTTC 
ACAATCGATG GTCTTAAGGG GCTTGAGAAG CTCTCCATTT TGGTTTCAGA GGGTGATCCC 
ACATCTTTAT GTTCCCTGCG TCTAATTGGT TGCTCTGATC TTGAATCTAT CGAATTGCAC 
GCTCTCAACT TGGAGTCTTG TTTGATTGAT AGGTGCTTCA ACCTTAGGTC GTTGGCACAC 
ACACACTCAT ATGTACAGGA GTTGAAATTA TGGGCTTGTC CAGAATTGTT GTTTCAGAGA 
GAGGGTTTGC CTTCCAACCT ACGTAAACTT GAAATTGGCG AGTGCAACCA ACTCACGCCC 
CAGGTGGAGT GGGGTTTGCA AAGGCTGACC TCTCTTACTC ATTTCACAAT CACCGGTGGA 
TGTGAAGACA TTGAATTATT TCCCAAGGAG TGTTTGCTGC CCTCTTCTCT GACTTCTCTT 
CAAATAGAAA TGCTTCCCAA TCTCAAGTCT CTTGACAGCG GGGGTCTTCA ACAGCTCACC 
TCTCTAAAAC GATTAGATAT TTATGGATGC TCGAGGCTCC AATCATTGAC AGAAGCGGGT 
CTTCAACACC TCACCTCTCT TGAAACGTTG TGGATCGCAC ACTGCCCAGT GCTCCAATCA 
TTGACAGAAG CGGGTCTTCA ACACCTCACC TCTCTTGAAA CGTTGTGGAT CCTAGACTGC 
CCAGTGCTCC AATCATTGAC AGAAGCGGGT ATTCAACACC TCACCTCTCT TGAAACGTTA 
GTGATCGAAA ACTGCCCAAT GCTGCAATCC TTGACAAAAG TGGGTCTTCA ACACCTCACC 
TCTCTTAAAG CATTGTATAT CAAAAACTGT TGTAAGCTCA AATACTTGAC AAAAGAAAGA 
CTTCCAGACT CCCTCTCTCT TCTGAGGATC TCCGAGTGTC CTTTACTGGA AAAACGGTGT 
CAATTTGAGA AAGGGAAAGA ATGGCGTTAT ATAGCTCACG TTCCAAAAAT AATGATCAAT 
GGTGTGCTAT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100853412 ( XM_019217714.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073259.1 Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X4, mRNA.

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CloneID	OVa39245
Clone ID Related Accession (Same CDS sequence)	XM_019217714.1
Accession Version	XM_019217714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative disease resistance RPP13-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATGC TGGCTTCTGG CATGTCAAAT GGTCATGACT ACTCTGCCAA CCATGAAGAC 
TCAAGTGCAA AGGGCAAAAA TGGTGAAGGA GAACTGCACA CATCAAAAGA TCTTCTAAAG 
AAACTGAAGA GATATGGAAT TTCTGGAATA TTATCCTATG GACTCCTGAA TACTGCATAC 
TATCTTACAA CGTTTCTCTT GGTATGGTTT TATATTGCTC CAGCACCAGG AAGGATGGGT 
TATTTGGCGG CAGTTGAAAG ATTTGTCAAA ATCATGGCTA TGGTGTGGGC AGGCAGCCAG 
GTCACTAAAC TTGTCAGAGC TGGAGGGGCC CTTGCACTTG CACCTATTGT AGATAGAGGA 
TTGTCATGGT TTACATTAAA ATTCAAGTTT GAGTCTCAGG GAAAGTTTGG TGGTATCGCG 
GTAACAGACA CCAATCCTCT GAATTCCTAT TGCAAAGCAA CAGTCGACAC AGATATGGCG 
GACGTCCTCC TCTCAGCTTC GCTTCAAGTT CTATTCGAAA GGTTGGCTTC TCCAGAGCTC 
ATAAACTTCA TTCGGCGACG GAACCTTAGC GATGAACTCC TCAGCGAGTT GAAGAGGAAA 
CTCGTGGTTG TTCTCAATGT GCTCGATGAT GCGGAGGTGA AGCAATTTTC CAACCCAAAT 
GTCAAAGAGT GGCTCGTCCA TGTCAAGGGT GCTGTGTATG ATGCGGAAGA CCTGTTGGAC 
GAGATCGCTA CCGACGCTTT GCGTTGCAAG ATGGAAGCTG CTGACTCCCA AACCGGCGGA 
ACTCTTAAGG CGTGGAAATG GAACAAGTTC TCTGCTTCTG TGAAGACTCC ATTTGCTATC 
AAAAGCATGG AGTCCAGGGT CAGGGGCATG ATTGATCTAC TGGAAAAAAT TGCACTAGAA 
AAAGTTGGGT TGGGGCTGGC AGAGGGTGGG GGCGAGAAAC GGTCACCAAG ACCAAGATCA 
CCAATATCCA CTTCTTTGGA GGATGACTCC ATTGTTGTCG GCAGGGATGA AATTCAGAAG 
GAGATGGTGG AGTGGTTGCT TTCTGACAAT ACAACAGGCG ACAAAATGGG GGTGATGTCC 
ATAGTGGGCA TGGGCGGCAG CGGCAAGACC ACGCTTGCTC GGCGTCTCTA TAACGATGAG 
GAAGTGAAGA AACACTTCGA CTTGCAAGCA TGGGTCTGTG TTTCCACTGA GTTTCTCCTT 
ATCAAACTCA CCAAAACAAT TCTTGAGGAA ATTCGTTCTC CACCTACTTC CGCTGACAAC 
CTAAATTTGC TTCAGCTTCA ACTCAAAGAG CAACTTAGTA ACAAGAAATT TCTGCTTGTT 
CTTGATGACG TCTGGAATTT GAATCCTCGT GATGAAGGTT ATATGGAGCT TAGTGATCGT 
GAAGGTTGGG AACGTCTACG AACTCCACTC CTCGCTGCAG CAGAGGGAAG CAAGATTGTT 
GTGACCAGTC GTAATAAATC TGTTGCAGAA GCCATGAAAG CGGCCCCTAC TCATGATCTT 
GGGAAATTAA GCTCTGAAGA TAGTTGGTCC CTGTTTAAAA AGCATGCATT TGGAGATAGA 
GACCCGAACG CATTCCTTGA GCTTGAACGC ATAGGCAGAC AGATTGTGGA CAAGTGTCAA 
GGATTGCCTT TGGCTGTGAA AGCACTCGGC TGTCTCTTGT ATTCTAAGGA CGAAAAAATG 
GAATGGGACG ATGTGTTGAG AAGTGAAATA TGGCATCCGC AGCGTGGCTC CGAAATTCTT 
CCGTCTTTGA TATTAAGCTA CCATCATCTT TCTTTACCTC TGAAGCATTG TTTTGCTTAT 
TGTTCTATTT TTCCCCAGGA CCACCAATTC AACAAAGAGA AGCTGATTTT ATTATGGATG 
GCAGAAGGTC TTTTACATCC ACAACAAAAC GAAGGAAGGA GAATGGAAGA GATCGGTGAG 
TCATATTTTG ATGAGCTTCT TGCCAAGTCT TTCTTTCAAA AATCTATTGG AAGAAAAGGA 
TCATGCTTTG TAATGCATGA TCTAATACAT GAATTGGCTC AACATGTGTC CGGAGATTTT 
TGTGCTCGAG TGGAAGATGA TGATAAATTA CCAAAAGTAT CTGAGAAGGC TCACCACTTT 
TTGTACTTTA ATAGTGATTA TAGCTATTTG GTAGCATTTA AGAATTTTGA GGCTATGACT 
AAAGCTAAAT CTCTTCGAAC ATTCTTAGGA GTGAAGCCAA CGGAACACTA TCCTTCTTAT 
ACTTTGAGTA AAAGAGTTTT GCAAGATATA TTACCAAAAA TGTGGTGTCT ACGCGTGTTA 
TCATTATGTG CATATGAGAT AACTGATTTG CCTAAATCGA TAGGTAATTT GAAACATTTG 
CGTTACTTGG ATTTGTCTTT CACGAGGATT AAAAAATTAC CCGAATCAGT ATGTTGTTTA 
TGCAATTTAC AAACGATGAT GCTGGGAGGA TGTTCTAGAC TTGATGAATT GCCTTCAAAG 
ATGGGAAAAT TGATTTATTT GCGCTATCTT GATATTGATG GATGTAATTC ATTGAGAGAA 
ATGTCAAGTC ATGGAATTGA TCGATTAAAA AATTTACAAA GGTTGACTCA ATTTAATGTG 
GGCCAAAACA ATGGATTAAG AATTGGAGAA TTGGGGGAGC TTTCAGAGAT TCGAGGAAAA 
CTTCATATTT CAAACATGGA GAATGTGGTG AGTGTTGATG ATGCATCAAG GGCTAATATG 
AAGGATAAAA GTTATCTTGA TGAGTTAATC TTTGATTGGT GTACCAGTGG TGTTACACAA 
AGTGGTGCAA CAACGCATGA TATACTCAAC AAGTTACAAC CTCATCCAAA TTTAAAACAA 
CTCTCCATCA AACACTATCC TGGTGAAGGA TTTCCAAATT GGCTTGGAGA TCCTTCAGTG 
TTGAATCTCG TGTCCCTTGA GCTTCGGGGT TGTGGCAATT GCTCAACATT GCCACCACTT 
GGACAGCTAA CCCAGCTTAA ATATCTGCAA ATCTCAGGGA TGAATGGAGT AGAGTGTGTG 
GGTGATGAGT TCTATGGGAA TGCTTCCTTT CAATTCCTAG AAACACTATC ATTTGAGGAT 
ATGCAGAACT GGGAGAAATG GTTATGTTGT GGAGAATTCC CTCGTCTCCA AAAGCTTTTT 
ATACGAAGGT GTCCCAAACT CACTGGGAAA TTACCAGAAC AGCTTCTTTC ATTAGTGGAA 
CTTCAAATTC ATGAATGTCC GCAACTGCTT ATGGCTTCAC TCACAGTTCC TATAATTCGT 
CAGTTGAGGA TGGTGGATTT CGGTAAACTG CAGTTGCAAA TGCCGGGTTG TGACTTCACA 
GCTCTTCAAA CTTCAGAAAT TGAAATTTTA GATGTGTCTC AGTGGAGTCA ACTTCCAATG 
GCACCACACC AGCTCTCAAT TAGAGAATGT GATAATGCGG AGTCTCTGCT AGAGGAGGAA 
ATCTCGCAAA CCAACATACA TGATTGCAGT TTTTCTAGAT CTCTGCACAA AGTTGGTTTA 
CCCACTACAC TGAAATCACT ATTCATCTCT GAGTGCTCGA AATTAGAGAT TCTCGTACCT 
GAGTTGTCCA GATGCCATCT CCCAGTCCTT GAAAGTCTAG AAATCAAAGG TGGTGTTATC 
GATGATTCTC TCACGTTATC CTTCTCATTA GGCATCTTCC CCAAGTTGAC TGATTTCACA 
ATCGATGGTC TTAAGGGGCT TGAGAAGCTC TCCATTTTGG TTTCAGAGGG TGATCCCACA 
TCTTTATGTT CCCTGCGTCT AATTGGTTGC TCTGATCTTG AATCTATCGA ATTGCACGCT 
CTCAACTTGG AGTCTTGTTT GATTGATAGG TGCTTCAACC TTAGGTCGTT GGCACACACA 
CACTCATATG TACAGGAGTT GAAATTATGG GCTTGTCCAG AATTGTTGTT TCAGAGAGAG 
GGTTTGCCTT CCAACCTACG TAAACTTGAA ATTGGCGAGT GCAACCAACT CACGCCCCAG 
GTGGAGTGGG GTTTGCAAAG GCTGACCTCT CTTACTCATT TCACAATCAC CGGTGGATGT 
GAAGACATTG AATTATTTCC CAAGGAGTGT TTGCTGCCCT CTTCTCTGAC TTCTCTTCAA 
ATAGAAATGC TTCCCAATCT CAAGTCTCTT GACAGCGGGG GTCTTCAACA GCTCACCTCT 
CTAAAACGAT TAGATATTTA TGGATGCTCG AGGCTCCAAT CATTGACAGA AGCGGGTCTT 
CAACACCTCA CCTCTCTTGA AACGTTGTGG ATCGCACACT GCCCAGTGCT CCAATCATTG 
ACAGAAGCGG GTCTTCAACA CCTCACCTCT CTTGAAACGT TGTGGATCCT AGACTGCCCA 
GTGCTCCAAT CATTGACAGA AGCGGGTATT CAACACCTCA CCTCTCTTGA AACGTTAGTG 
ATCGAAAACT GCCCAATGCT GCAATCCTTG ACAAAAGTGG GTCTTCAACA CCTCACCTCT 
CTTAAAGCAT TGTATATCAA AAACTGTTGT AAGCTCAAAT ACTTGACAAA AGAAAGACTT 
CCAGACTCCC TCTCTCTTCT GAGGATCTCC GAGTGTCCTT TACTGGAAAA ACGGTGTCAA 
TTTGAGAAAG GGAAAGAATG GCGTTATATA GCTCACGTTC CAAAAATAAT GATCAATGGT 
GTGCTATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073259.1
CDS	202..4833
Translation	MAMLASGMSNGHDYSANHEDSSAKGKNGEGELHTSKDLLKKLKRYGISGILSYGLLNTAYYLTTFLLVWFYIAPAPGRMGYLAAVERFVKIMAMVWAGSQVTKLVRAGGALALAPIVDRGLSWFTLKFKFESQGKFGGIAVTDTNPLNSYCKATVDTDMADVLLSASLQVLFERLASPELINFIRRRNLSDELLSELKRKLVVVLNVLDDAEVKQFSNPNVKEWLVHVKGAVYDAEDLLDEIATDALRCKMEAADSQTGGTLKAWKWNKFSASVKTPFAIKSMESRVRGMIDLLEKIALEKVGLGLAEGGGEKRSPRPRSPISTSLEDDSIVVGRDEIQKEMVEWLLSDNTTGDKMGVMSIVGMGGSGKTTLARRLYNDEEVKKHFDLQAWVCVSTEFLLIKLTKTILEEIRSPPTSADNLNLLQLQLKEQLSNKKFLLVLDDVWNLNPRDEGYMELSDREGWERLRTPLLAAAEGSKIVVTSRNKSVAEAMKAAPTHDLGKLSSEDSWSLFKKHAFGDRDPNAFLELERIGRQIVDKCQGLPLAVKALGCLLYSKDEKMEWDDVLRSEIWHPQRGSEILPSLILSYHHLSLPLKHCFAYCSIFPQDHQFNKEKLILLWMAEGLLHPQQNEGRRMEEIGESYFDELLAKSFFQKSIGRKGSCFVMHDLIHELAQHVSGDFCARVEDDDKLPKVSEKAHHFLYFNSDYSYLVAFKNFEAMTKAKSLRTFLGVKPTEHYPSYTLSKRVLQDILPKMWCLRVLSLCAYEITDLPKSIGNLKHLRYLDLSFTRIKKLPESVCCLCNLQTMMLGGCSRLDELPSKMGKLIYLRYLDIDGCNSLREMSSHGIDRLKNLQRLTQFNVGQNNGLRIGELGELSEIRGKLHISNMENVVSVDDASRANMKDKSYLDELIFDWCTSGVTQSGATTHDILNKLQPHPNLKQLSIKHYPGEGFPNWLGDPSVLNLVSLELRGCGNCSTLPPLGQLTQLKYLQISGMNGVECVGDEFYGNASFQFLETLSFEDMQNWEKWLCCGEFPRLQKLFIRRCPKLTGKLPEQLLSLVELQIHECPQLLMASLTVPIIRQLRMVDFGKLQLQMPGCDFTALQTSEIEILDVSQWSQLPMAPHQLSIRECDNAESLLEEEISQTNIHDCSFSRSLHKVGLPTTLKSLFISECSKLEILVPELSRCHLPVLESLEIKGGVIDDSLTLSFSLGIFPKLTDFTIDGLKGLEKLSILVSEGDPTSLCSLRLIGCSDLESIELHALNLESCLIDRCFNLRSLAHTHSYVQELKLWACPELLFQREGLPSNLRKLEIGECNQLTPQVEWGLQRLTSLTHFTITGGCEDIELFPKECLLPSSLTSLQIEMLPNLKSLDSGGLQQLTSLKRLDIYGCSRLQSLTEAGLQHLTSLETLWIAHCPVLQSLTEAGLQHLTSLETLWILDCPVLQSLTEAGIQHLTSLETLVIENCPMLQSLTKVGLQHLTSLKALYIKNCCKLKYLTKERLPDSLSLLRISECPLLEKRCQFEKGKEWRYIAHVPKIMINGVLY

Target ORF information:

RefSeq Version	XM_019217714.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217714.1

ORF Insert Sequence:

ATGGCCATGC TGGCTTCTGG CATGTCAAAT GGTCATGACT ACTCTGCCAA CCATGAAGAC 
TCAAGTGCAA AGGGCAAAAA TGGTGAAGGA GAACTGCACA CATCAAAAGA TCTTCTAAAG 
AAACTGAAGA GATATGGAAT TTCTGGAATA TTATCCTATG GACTCCTGAA TACTGCATAC 
TATCTTACAA CGTTTCTCTT GGTATGGTTT TATATTGCTC CAGCACCAGG AAGGATGGGT 
TATTTGGCGG CAGTTGAAAG ATTTGTCAAA ATCATGGCTA TGGTGTGGGC AGGCAGCCAG 
GTCACTAAAC TTGTCAGAGC TGGAGGGGCC CTTGCACTTG CACCTATTGT AGATAGAGGA 
TTGTCATGGT TTACATTAAA ATTCAAGTTT GAGTCTCAGG GAAAGTTTGG TGGTATCGCG 
GTAACAGACA CCAATCCTCT GAATTCCTAT TGCAAAGCAA CAGTCGACAC AGATATGGCG 
GACGTCCTCC TCTCAGCTTC GCTTCAAGTT CTATTCGAAA GGTTGGCTTC TCCAGAGCTC 
ATAAACTTCA TTCGGCGACG GAACCTTAGC GATGAACTCC TCAGCGAGTT GAAGAGGAAA 
CTCGTGGTTG TTCTCAATGT GCTCGATGAT GCGGAGGTGA AGCAATTTTC CAACCCAAAT 
GTCAAAGAGT GGCTCGTCCA TGTCAAGGGT GCTGTGTATG ATGCGGAAGA CCTGTTGGAC 
GAGATCGCTA CCGACGCTTT GCGTTGCAAG ATGGAAGCTG CTGACTCCCA AACCGGCGGA 
ACTCTTAAGG CGTGGAAATG GAACAAGTTC TCTGCTTCTG TGAAGACTCC ATTTGCTATC 
AAAAGCATGG AGTCCAGGGT CAGGGGCATG ATTGATCTAC TGGAAAAAAT TGCACTAGAA 
AAAGTTGGGT TGGGGCTGGC AGAGGGTGGG GGCGAGAAAC GGTCACCAAG ACCAAGATCA 
CCAATATCCA CTTCTTTGGA GGATGACTCC ATTGTTGTCG GCAGGGATGA AATTCAGAAG 
GAGATGGTGG AGTGGTTGCT TTCTGACAAT ACAACAGGCG ACAAAATGGG GGTGATGTCC 
ATAGTGGGCA TGGGCGGCAG CGGCAAGACC ACGCTTGCTC GGCGTCTCTA TAACGATGAG 
GAAGTGAAGA AACACTTCGA CTTGCAAGCA TGGGTCTGTG TTTCCACTGA GTTTCTCCTT 
ATCAAACTCA CCAAAACAAT TCTTGAGGAA ATTCGTTCTC CACCTACTTC CGCTGACAAC 
CTAAATTTGC TTCAGCTTCA ACTCAAAGAG CAACTTAGTA ACAAGAAATT TCTGCTTGTT 
CTTGATGACG TCTGGAATTT GAATCCTCGT GATGAAGGTT ATATGGAGCT TAGTGATCGT 
GAAGGTTGGG AACGTCTACG AACTCCACTC CTCGCTGCAG CAGAGGGAAG CAAGATTGTT 
GTGACCAGTC GTAATAAATC TGTTGCAGAA GCCATGAAAG CGGCCCCTAC TCATGATCTT 
GGGAAATTAA GCTCTGAAGA TAGTTGGTCC CTGTTTAAAA AGCATGCATT TGGAGATAGA 
GACCCGAACG CATTCCTTGA GCTTGAACGC ATAGGCAGAC AGATTGTGGA CAAGTGTCAA 
GGATTGCCTT TGGCTGTGAA AGCACTCGGC TGTCTCTTGT ATTCTAAGGA CGAAAAAATG 
GAATGGGACG ATGTGTTGAG AAGTGAAATA TGGCATCCGC AGCGTGGCTC CGAAATTCTT 
CCGTCTTTGA TATTAAGCTA CCATCATCTT TCTTTACCTC TGAAGCATTG TTTTGCTTAT 
TGTTCTATTT TTCCCCAGGA CCACCAATTC AACAAAGAGA AGCTGATTTT ATTATGGATG 
GCAGAAGGTC TTTTACATCC ACAACAAAAC GAAGGAAGGA GAATGGAAGA GATCGGTGAG 
TCATATTTTG ATGAGCTTCT TGCCAAGTCT TTCTTTCAAA AATCTATTGG AAGAAAAGGA 
TCATGCTTTG TAATGCATGA TCTAATACAT GAATTGGCTC AACATGTGTC CGGAGATTTT 
TGTGCTCGAG TGGAAGATGA TGATAAATTA CCAAAAGTAT CTGAGAAGGC TCACCACTTT 
TTGTACTTTA ATAGTGATTA TAGCTATTTG GTAGCATTTA AGAATTTTGA GGCTATGACT 
AAAGCTAAAT CTCTTCGAAC ATTCTTAGGA GTGAAGCCAA CGGAACACTA TCCTTCTTAT 
ACTTTGAGTA AAAGAGTTTT GCAAGATATA TTACCAAAAA TGTGGTGTCT ACGCGTGTTA 
TCATTATGTG CATATGAGAT AACTGATTTG CCTAAATCGA TAGGTAATTT GAAACATTTG 
CGTTACTTGG ATTTGTCTTT CACGAGGATT AAAAAATTAC CCGAATCAGT ATGTTGTTTA 
TGCAATTTAC AAACGATGAT GCTGGGAGGA TGTTCTAGAC TTGATGAATT GCCTTCAAAG 
ATGGGAAAAT TGATTTATTT GCGCTATCTT GATATTGATG GATGTAATTC ATTGAGAGAA 
ATGTCAAGTC ATGGAATTGA TCGATTAAAA AATTTACAAA GGTTGACTCA ATTTAATGTG 
GGCCAAAACA ATGGATTAAG AATTGGAGAA TTGGGGGAGC TTTCAGAGAT TCGAGGAAAA 
CTTCATATTT CAAACATGGA GAATGTGGTG AGTGTTGATG ATGCATCAAG GGCTAATATG 
AAGGATAAAA GTTATCTTGA TGAGTTAATC TTTGATTGGT GTACCAGTGG TGTTACACAA 
AGTGGTGCAA CAACGCATGA TATACTCAAC AAGTTACAAC CTCATCCAAA TTTAAAACAA 
CTCTCCATCA AACACTATCC TGGTGAAGGA TTTCCAAATT GGCTTGGAGA TCCTTCAGTG 
TTGAATCTCG TGTCCCTTGA GCTTCGGGGT TGTGGCAATT GCTCAACATT GCCACCACTT 
GGACAGCTAA CCCAGCTTAA ATATCTGCAA ATCTCAGGGA TGAATGGAGT AGAGTGTGTG 
GGTGATGAGT TCTATGGGAA TGCTTCCTTT CAATTCCTAG AAACACTATC ATTTGAGGAT 
ATGCAGAACT GGGAGAAATG GTTATGTTGT GGAGAATTCC CTCGTCTCCA AAAGCTTTTT 
ATACGAAGGT GTCCCAAACT CACTGGGAAA TTACCAGAAC AGCTTCTTTC ATTAGTGGAA 
CTTCAAATTC ATGAATGTCC GCAACTGCTT ATGGCTTCAC TCACAGTTCC TATAATTCGT 
CAGTTGAGGA TGGTGGATTT CGGTAAACTG CAGTTGCAAA TGCCGGGTTG TGACTTCACA 
GCTCTTCAAA CTTCAGAAAT TGAAATTTTA GATGTGTCTC AGTGGAGTCA ACTTCCAATG 
GCACCACACC AGCTCTCAAT TAGAGAATGT GATAATGCGG AGTCTCTGCT AGAGGAGGAA 
ATCTCGCAAA CCAACATACA TGATTGCAGT TTTTCTAGAT CTCTGCACAA AGTTGGTTTA 
CCCACTACAC TGAAATCACT ATTCATCTCT GAGTGCTCGA AATTAGAGAT TCTCGTACCT 
GAGTTGTCCA GATGCCATCT CCCAGTCCTT GAAAGTCTAG AAATCAAAGG TGGTGTTATC 
GATGATTCTC TCACGTTATC CTTCTCATTA GGCATCTTCC CCAAGTTGAC TGATTTCACA 
ATCGATGGTC TTAAGGGGCT TGAGAAGCTC TCCATTTTGG TTTCAGAGGG TGATCCCACA 
TCTTTATGTT CCCTGCGTCT AATTGGTTGC TCTGATCTTG AATCTATCGA ATTGCACGCT 
CTCAACTTGG AGTCTTGTTT GATTGATAGG TGCTTCAACC TTAGGTCGTT GGCACACACA 
CACTCATATG TACAGGAGTT GAAATTATGG GCTTGTCCAG AATTGTTGTT TCAGAGAGAG 
GGTTTGCCTT CCAACCTACG TAAACTTGAA ATTGGCGAGT GCAACCAACT CACGCCCCAG 
GTGGAGTGGG GTTTGCAAAG GCTGACCTCT CTTACTCATT TCACAATCAC CGGTGGATGT 
GAAGACATTG AATTATTTCC CAAGGAGTGT TTGCTGCCCT CTTCTCTGAC TTCTCTTCAA 
ATAGAAATGC TTCCCAATCT CAAGTCTCTT GACAGCGGGG GTCTTCAACA GCTCACCTCT 
CTAAAACGAT TAGATATTTA TGGATGCTCG AGGCTCCAAT CATTGACAGA AGCGGGTCTT 
CAACACCTCA CCTCTCTTGA AACGTTGTGG ATCGCACACT GCCCAGTGCT CCAATCATTG 
ACAGAAGCGG GTCTTCAACA CCTCACCTCT CTTGAAACGT TGTGGATCCT AGACTGCCCA 
GTGCTCCAAT CATTGACAGA AGCGGGTATT CAACACCTCA CCTCTCTTGA AACGTTAGTG 
ATCGAAAACT GCCCAATGCT GCAATCCTTG ACAAAAGTGG GTCTTCAACA CCTCACCTCT 
CTTAAAGCAT TGTATATCAA AAACTGTTGT AAGCTCAAAT ACTTGACAAA AGAAAGACTT 
CCAGACTCCC TCTCTCTTCT GAGGATCTCC GAGTGTCCTT TACTGGAAAA ACGGTGTCAA 
TTTGAGAAAG GGAAAGAATG GCGTTATATA GCTCACGTTC CAAAAATAAT GATCAATGGT 
GTGCTATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100853412 ( XM_019217715.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073260.1 Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X5, mRNA.

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CloneID	OVa39246
Clone ID Related Accession (Same CDS sequence)	XM_019217715.1
Accession Version	XM_019217715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative disease resistance RPP13-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAATG GTCATGACTA CTCTGCCAAC CATGAAGACT CAAGTGCAAA GGGCAAAAAT 
GGTGAAGGAG AACTGCACAC ATCAAAAGAT CTTCTAAAGA AACTGAAGAG ATATGGAATT 
TCTGGAATAT TATCCTATGG ACTCCTGAAT ACTGCATACT ATCTTACAAC GTTTCTCTTG 
GTATGGTTTT ATATTGCTCC AGCACCAGGA AGGATGGGTT ATTTGGCGGC AGTTGAAAGA 
TTTGTCAAAA TCATGGCTAT GGTGTGGGCA GGCAGCCAGG TCACTAAACT TGTCAGAGCT 
GGAGGGGCCC TTGCACTTGC ACCTATTGTA GATAGAGGAT TGTCATGGTT TACATTAAAA 
TTCAAGTTTG AGTCTCAGGG AAAGTTTGGT GGTATCGCGG TAACAGACAC CAATCCTCTG 
AATTCCTATT GCAAAGCAAC AGTCGACACA GATATGGCGG ACGTCCTCCT CTCAGCTTCG 
CTTCAAGTTC TATTCGAAAG GTTGGCTTCT CCAGAGCTCA TAAACTTCAT TCGGCGACGG 
AACCTTAGCG ATGAACTCCT CAGCGAGTTG AAGAGGAAAC TCGTGGTTGT TCTCAATGTG 
CTCGATGATG CGGAGGTGAA GCAATTTTCC AACCCAAATG TCAAAGAGTG GCTCGTCCAT 
GTCAAGGGTG CTGTGTATGA TGCGGAAGAC CTGTTGGACG AGATCGCTAC CGACGCTTTG 
CGTTGCAAGA TGGAAGCTGC TGACTCCCAA ACCGGCGGAA CTCTTAAGGC GTGGAAATGG 
AACAAGTTCT CTGCTTCTGT GAAGACTCCA TTTGCTATCA AAAGCATGGA GTCCAGGGTC 
AGGGGCATGA TTGATCTACT GGAAAAAATT GCACTAGAAA AAGTTGGGTT GGGGCTGGCA 
GAGGGTGGGG GCGAGAAACG GTCACCAAGA CCAAGATCAC CAATATCCAC TTCTTTGGAG 
GATGACTCCA TTGTTGTCGG CAGGGATGAA ATTCAGAAGG AGATGGTGGA GTGGTTGCTT 
TCTGACAATA CAACAGGCGA CAAAATGGGG GTGATGTCCA TAGTGGGCAT GGGCGGCAGC 
GGCAAGACCA CGCTTGCTCG GCGTCTCTAT AACGATGAGG AAGTGAAGAA ACACTTCGAC 
TTGCAAGCAT GGGTCTGTGT TTCCACTGAG TTTCTCCTTA TCAAACTCAC CAAAACAATT 
CTTGAGGAAA TTCGTTCTCC ACCTACTTCC GCTGACAACC TAAATTTGCT TCAGCTTCAA 
CTCAAAGAGC AACTTAGTAA CAAGAAATTT CTGCTTGTTC TTGATGACGT CTGGAATTTG 
AATCCTCGTG ATGAAGGTTA TATGGAGCTT AGTGATCGTG AAGGTTGGGA ACGTCTACGA 
ACTCCACTCC TCGCTGCAGC AGAGGGAAGC AAGATTGTTG TGACCAGTCG TAATAAATCT 
GTTGCAGAAG CCATGAAAGC GGCCCCTACT CATGATCTTG GGAAATTAAG CTCTGAAGAT 
AGTTGGTCCC TGTTTAAAAA GCATGCATTT GGAGATAGAG ACCCGAACGC ATTCCTTGAG 
CTTGAACGCA TAGGCAGACA GATTGTGGAC AAGTGTCAAG GATTGCCTTT GGCTGTGAAA 
GCACTCGGCT GTCTCTTGTA TTCTAAGGAC GAAAAAATGG AATGGGACGA TGTGTTGAGA 
AGTGAAATAT GGCATCCGCA GCGTGGCTCC GAAATTCTTC CGTCTTTGAT ATTAAGCTAC 
CATCATCTTT CTTTACCTCT GAAGCATTGT TTTGCTTATT GTTCTATTTT TCCCCAGGAC 
CACCAATTCA ACAAAGAGAA GCTGATTTTA TTATGGATGG CAGAAGGTCT TTTACATCCA 
CAACAAAACG AAGGAAGGAG AATGGAAGAG ATCGGTGAGT CATATTTTGA TGAGCTTCTT 
GCCAAGTCTT TCTTTCAAAA ATCTATTGGA AGAAAAGGAT CATGCTTTGT AATGCATGAT 
CTAATACATG AATTGGCTCA ACATGTGTCC GGAGATTTTT GTGCTCGAGT GGAAGATGAT 
GATAAATTAC CAAAAGTATC TGAGAAGGCT CACCACTTTT TGTACTTTAA TAGTGATTAT 
AGCTATTTGG TAGCATTTAA GAATTTTGAG GCTATGACTA AAGCTAAATC TCTTCGAACA 
TTCTTAGGAG TGAAGCCAAC GGAACACTAT CCTTCTTATA CTTTGAGTAA AAGAGTTTTG 
CAAGATATAT TACCAAAAAT GTGGTGTCTA CGCGTGTTAT CATTATGTGC ATATGAGATA 
ACTGATTTGC CTAAATCGAT AGGTAATTTG AAACATTTGC GTTACTTGGA TTTGTCTTTC 
ACGAGGATTA AAAAATTACC CGAATCAGTA TGTTGTTTAT GCAATTTACA AACGATGATG 
CTGGGAGGAT GTTCTAGACT TGATGAATTG CCTTCAAAGA TGGGAAAATT GATTTATTTG 
CGCTATCTTG ATATTGATGG ATGTAATTCA TTGAGAGAAA TGTCAAGTCA TGGAATTGAT 
CGATTAAAAA ATTTACAAAG GTTGACTCAA TTTAATGTGG GCCAAAACAA TGGATTAAGA 
ATTGGAGAAT TGGGGGAGCT TTCAGAGATT CGAGGAAAAC TTCATATTTC AAACATGGAG 
AATGTGGTGA GTGTTGATGA TGCATCAAGG GCTAATATGA AGGATAAAAG TTATCTTGAT 
GAGTTAATCT TTGATTGGTG TACCAGTGGT GTTACACAAA GTGGTGCAAC AACGCATGAT 
ATACTCAACA AGTTACAACC TCATCCAAAT TTAAAACAAC TCTCCATCAA ACACTATCCT 
GGTGAAGGAT TTCCAAATTG GCTTGGAGAT CCTTCAGTGT TGAATCTCGT GTCCCTTGAG 
CTTCGGGGTT GTGGCAATTG CTCAACATTG CCACCACTTG GACAGCTAAC CCAGCTTAAA 
TATCTGCAAA TCTCAGGGAT GAATGGAGTA GAGTGTGTGG GTGATGAGTT CTATGGGAAT 
GCTTCCTTTC AATTCCTAGA AACACTATCA TTTGAGGATA TGCAGAACTG GGAGAAATGG 
TTATGTTGTG GAGAATTCCC TCGTCTCCAA AAGCTTTTTA TACGAAGGTG TCCCAAACTC 
ACTGGGAAAT TACCAGAACA GCTTCTTTCA TTAGTGGAAC TTCAAATTCA TGAATGTCCG 
CAACTGCTTA TGGCTTCACT CACAGTTCCT ATAATTCGTC AGTTGAGGAT GGTGGATTTC 
GGTAAACTGC AGTTGCAAAT GCCGGGTTGT GACTTCACAG CTCTTCAAAC TTCAGAAATT 
GAAATTTTAG ATGTGTCTCA GTGGAGTCAA CTTCCAATGG CACCACACCA GCTCTCAATT 
AGAGAATGTG ATAATGCGGA GTCTCTGCTA GAGGAGGAAA TCTCGCAAAC CAACATACAT 
GATTGCAGTT TTTCTAGATC TCTGCACAAA GTTGGTTTAC CCACTACACT GAAATCACTA 
TTCATCTCTG AGTGCTCGAA ATTAGAGATT CTCGTACCTG AGTTGTCCAG ATGCCATCTC 
CCAGTCCTTG AAAGTCTAGA AATCAAAGGT GGTGTTATCG ATGATTCTCT CACGTTATCC 
TTCTCATTAG GCATCTTCCC CAAGTTGACT GATTTCACAA TCGATGGTCT TAAGGGGCTT 
GAGAAGCTCT CCATTTTGGT TTCAGAGGGT GATCCCACAT CTTTATGTTC CCTGCGTCTA 
ATTGGTTGCT CTGATCTTGA ATCTATCGAA TTGCACGCTC TCAACTTGGA GTCTTGTTTG 
ATTGATAGGT GCTTCAACCT TAGGTCGTTG GCACACACAC ACTCATATGT ACAGGAGTTG 
AAATTATGGG CTTGTCCAGA ATTGTTGTTT CAGAGAGAGG GTTTGCCTTC CAACCTACGT 
AAACTTGAAA TTGGCGAGTG CAACCAACTC ACGCCCCAGG TGGAGTGGGG TTTGCAAAGG 
CTGACCTCTC TTACTCATTT CACAATCACC GGTGGATGTG AAGACATTGA ATTATTTCCC 
AAGGAGTGTT TGCTGCCCTC TTCTCTGACT TCTCTTCAAA TAGAAATGCT TCCCAATCTC 
AAGTCTCTTG ACAGCGGGGG TCTTCAACAG CTCACCTCTC TAAAACGATT AGATATTTAT 
GGATGCTCGA GGCTCCAATC ATTGACAGAA GCGGGTCTTC AACACCTCAC CTCTCTTGAA 
ACGTTGTGGA TCGCACACTG CCCAGTGCTC CAATCATTGA CAGAAGCGGG TCTTCAACAC 
CTCACCTCTC TTGAAACGTT GTGGATCCTA GACTGCCCAG TGCTCCAATC ATTGACAGAA 
GCGGGTATTC AACACCTCAC CTCTCTTGAA ACGTTAGTGA TCGAAAACTG CCCAATGCTG 
CAATCCTTGA CAAAAGTGGG TCTTCAACAC CTCACCTCTC TTAAAGCATT GTATATCAAA 
AACTGTTGTA AGCTCAAATA CTTGACAAAA GAAAGACTTC CAGACTCCCT CTCTCTTCTG 
AGGATCTCCG AGTGTCCTTT ACTGGAAAAA CGGTGTCAAT TTGAGAAAGG GAAAGAATGG 
CGTTATATAG CTCACGTTCC AAAAATAATG ATCAATGGTG TGCTATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073260.1
CDS	228..4838
Translation	MSNGHDYSANHEDSSAKGKNGEGELHTSKDLLKKLKRYGISGILSYGLLNTAYYLTTFLLVWFYIAPAPGRMGYLAAVERFVKIMAMVWAGSQVTKLVRAGGALALAPIVDRGLSWFTLKFKFESQGKFGGIAVTDTNPLNSYCKATVDTDMADVLLSASLQVLFERLASPELINFIRRRNLSDELLSELKRKLVVVLNVLDDAEVKQFSNPNVKEWLVHVKGAVYDAEDLLDEIATDALRCKMEAADSQTGGTLKAWKWNKFSASVKTPFAIKSMESRVRGMIDLLEKIALEKVGLGLAEGGGEKRSPRPRSPISTSLEDDSIVVGRDEIQKEMVEWLLSDNTTGDKMGVMSIVGMGGSGKTTLARRLYNDEEVKKHFDLQAWVCVSTEFLLIKLTKTILEEIRSPPTSADNLNLLQLQLKEQLSNKKFLLVLDDVWNLNPRDEGYMELSDREGWERLRTPLLAAAEGSKIVVTSRNKSVAEAMKAAPTHDLGKLSSEDSWSLFKKHAFGDRDPNAFLELERIGRQIVDKCQGLPLAVKALGCLLYSKDEKMEWDDVLRSEIWHPQRGSEILPSLILSYHHLSLPLKHCFAYCSIFPQDHQFNKEKLILLWMAEGLLHPQQNEGRRMEEIGESYFDELLAKSFFQKSIGRKGSCFVMHDLIHELAQHVSGDFCARVEDDDKLPKVSEKAHHFLYFNSDYSYLVAFKNFEAMTKAKSLRTFLGVKPTEHYPSYTLSKRVLQDILPKMWCLRVLSLCAYEITDLPKSIGNLKHLRYLDLSFTRIKKLPESVCCLCNLQTMMLGGCSRLDELPSKMGKLIYLRYLDIDGCNSLREMSSHGIDRLKNLQRLTQFNVGQNNGLRIGELGELSEIRGKLHISNMENVVSVDDASRANMKDKSYLDELIFDWCTSGVTQSGATTHDILNKLQPHPNLKQLSIKHYPGEGFPNWLGDPSVLNLVSLELRGCGNCSTLPPLGQLTQLKYLQISGMNGVECVGDEFYGNASFQFLETLSFEDMQNWEKWLCCGEFPRLQKLFIRRCPKLTGKLPEQLLSLVELQIHECPQLLMASLTVPIIRQLRMVDFGKLQLQMPGCDFTALQTSEIEILDVSQWSQLPMAPHQLSIRECDNAESLLEEEISQTNIHDCSFSRSLHKVGLPTTLKSLFISECSKLEILVPELSRCHLPVLESLEIKGGVIDDSLTLSFSLGIFPKLTDFTIDGLKGLEKLSILVSEGDPTSLCSLRLIGCSDLESIELHALNLESCLIDRCFNLRSLAHTHSYVQELKLWACPELLFQREGLPSNLRKLEIGECNQLTPQVEWGLQRLTSLTHFTITGGCEDIELFPKECLLPSSLTSLQIEMLPNLKSLDSGGLQQLTSLKRLDIYGCSRLQSLTEAGLQHLTSLETLWIAHCPVLQSLTEAGLQHLTSLETLWILDCPVLQSLTEAGIQHLTSLETLVIENCPMLQSLTKVGLQHLTSLKALYIKNCCKLKYLTKERLPDSLSLLRISECPLLEKRCQFEKGKEWRYIAHVPKIMINGVLY

Target ORF information:

RefSeq Version	XM_019217715.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative disease resistance RPP13-like protein 1 (LOC100853412), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217715.1

ORF Insert Sequence:

ATGTCAAATG GTCATGACTA CTCTGCCAAC CATGAAGACT CAAGTGCAAA GGGCAAAAAT 
GGTGAAGGAG AACTGCACAC ATCAAAAGAT CTTCTAAAGA AACTGAAGAG ATATGGAATT 
TCTGGAATAT TATCCTATGG ACTCCTGAAT ACTGCATACT ATCTTACAAC GTTTCTCTTG 
GTATGGTTTT ATATTGCTCC AGCACCAGGA AGGATGGGTT ATTTGGCGGC AGTTGAAAGA 
TTTGTCAAAA TCATGGCTAT GGTGTGGGCA GGCAGCCAGG TCACTAAACT TGTCAGAGCT 
GGAGGGGCCC TTGCACTTGC ACCTATTGTA GATAGAGGAT TGTCATGGTT TACATTAAAA 
TTCAAGTTTG AGTCTCAGGG AAAGTTTGGT GGTATCGCGG TAACAGACAC CAATCCTCTG 
AATTCCTATT GCAAAGCAAC AGTCGACACA GATATGGCGG ACGTCCTCCT CTCAGCTTCG 
CTTCAAGTTC TATTCGAAAG GTTGGCTTCT CCAGAGCTCA TAAACTTCAT TCGGCGACGG 
AACCTTAGCG ATGAACTCCT CAGCGAGTTG AAGAGGAAAC TCGTGGTTGT TCTCAATGTG 
CTCGATGATG CGGAGGTGAA GCAATTTTCC AACCCAAATG TCAAAGAGTG GCTCGTCCAT 
GTCAAGGGTG CTGTGTATGA TGCGGAAGAC CTGTTGGACG AGATCGCTAC CGACGCTTTG 
CGTTGCAAGA TGGAAGCTGC TGACTCCCAA ACCGGCGGAA CTCTTAAGGC GTGGAAATGG 
AACAAGTTCT CTGCTTCTGT GAAGACTCCA TTTGCTATCA AAAGCATGGA GTCCAGGGTC 
AGGGGCATGA TTGATCTACT GGAAAAAATT GCACTAGAAA AAGTTGGGTT GGGGCTGGCA 
GAGGGTGGGG GCGAGAAACG GTCACCAAGA CCAAGATCAC CAATATCCAC TTCTTTGGAG 
GATGACTCCA TTGTTGTCGG CAGGGATGAA ATTCAGAAGG AGATGGTGGA GTGGTTGCTT 
TCTGACAATA CAACAGGCGA CAAAATGGGG GTGATGTCCA TAGTGGGCAT GGGCGGCAGC 
GGCAAGACCA CGCTTGCTCG GCGTCTCTAT AACGATGAGG AAGTGAAGAA ACACTTCGAC 
TTGCAAGCAT GGGTCTGTGT TTCCACTGAG TTTCTCCTTA TCAAACTCAC CAAAACAATT 
CTTGAGGAAA TTCGTTCTCC ACCTACTTCC GCTGACAACC TAAATTTGCT TCAGCTTCAA 
CTCAAAGAGC AACTTAGTAA CAAGAAATTT CTGCTTGTTC TTGATGACGT CTGGAATTTG 
AATCCTCGTG ATGAAGGTTA TATGGAGCTT AGTGATCGTG AAGGTTGGGA ACGTCTACGA 
ACTCCACTCC TCGCTGCAGC AGAGGGAAGC AAGATTGTTG TGACCAGTCG TAATAAATCT 
GTTGCAGAAG CCATGAAAGC GGCCCCTACT CATGATCTTG GGAAATTAAG CTCTGAAGAT 
AGTTGGTCCC TGTTTAAAAA GCATGCATTT GGAGATAGAG ACCCGAACGC ATTCCTTGAG 
CTTGAACGCA TAGGCAGACA GATTGTGGAC AAGTGTCAAG GATTGCCTTT GGCTGTGAAA 
GCACTCGGCT GTCTCTTGTA TTCTAAGGAC GAAAAAATGG AATGGGACGA TGTGTTGAGA 
AGTGAAATAT GGCATCCGCA GCGTGGCTCC GAAATTCTTC CGTCTTTGAT ATTAAGCTAC 
CATCATCTTT CTTTACCTCT GAAGCATTGT TTTGCTTATT GTTCTATTTT TCCCCAGGAC 
CACCAATTCA ACAAAGAGAA GCTGATTTTA TTATGGATGG CAGAAGGTCT TTTACATCCA 
CAACAAAACG AAGGAAGGAG AATGGAAGAG ATCGGTGAGT CATATTTTGA TGAGCTTCTT 
GCCAAGTCTT TCTTTCAAAA ATCTATTGGA AGAAAAGGAT CATGCTTTGT AATGCATGAT 
CTAATACATG AATTGGCTCA ACATGTGTCC GGAGATTTTT GTGCTCGAGT GGAAGATGAT 
GATAAATTAC CAAAAGTATC TGAGAAGGCT CACCACTTTT TGTACTTTAA TAGTGATTAT 
AGCTATTTGG TAGCATTTAA GAATTTTGAG GCTATGACTA AAGCTAAATC TCTTCGAACA 
TTCTTAGGAG TGAAGCCAAC GGAACACTAT CCTTCTTATA CTTTGAGTAA AAGAGTTTTG 
CAAGATATAT TACCAAAAAT GTGGTGTCTA CGCGTGTTAT CATTATGTGC ATATGAGATA 
ACTGATTTGC CTAAATCGAT AGGTAATTTG AAACATTTGC GTTACTTGGA TTTGTCTTTC 
ACGAGGATTA AAAAATTACC CGAATCAGTA TGTTGTTTAT GCAATTTACA AACGATGATG 
CTGGGAGGAT GTTCTAGACT TGATGAATTG CCTTCAAAGA TGGGAAAATT GATTTATTTG 
CGCTATCTTG ATATTGATGG ATGTAATTCA TTGAGAGAAA TGTCAAGTCA TGGAATTGAT 
CGATTAAAAA ATTTACAAAG GTTGACTCAA TTTAATGTGG GCCAAAACAA TGGATTAAGA 
ATTGGAGAAT TGGGGGAGCT TTCAGAGATT CGAGGAAAAC TTCATATTTC AAACATGGAG 
AATGTGGTGA GTGTTGATGA TGCATCAAGG GCTAATATGA AGGATAAAAG TTATCTTGAT 
GAGTTAATCT TTGATTGGTG TACCAGTGGT GTTACACAAA GTGGTGCAAC AACGCATGAT 
ATACTCAACA AGTTACAACC TCATCCAAAT TTAAAACAAC TCTCCATCAA ACACTATCCT 
GGTGAAGGAT TTCCAAATTG GCTTGGAGAT CCTTCAGTGT TGAATCTCGT GTCCCTTGAG 
CTTCGGGGTT GTGGCAATTG CTCAACATTG CCACCACTTG GACAGCTAAC CCAGCTTAAA 
TATCTGCAAA TCTCAGGGAT GAATGGAGTA GAGTGTGTGG GTGATGAGTT CTATGGGAAT 
GCTTCCTTTC AATTCCTAGA AACACTATCA TTTGAGGATA TGCAGAACTG GGAGAAATGG 
TTATGTTGTG GAGAATTCCC TCGTCTCCAA AAGCTTTTTA TACGAAGGTG TCCCAAACTC 
ACTGGGAAAT TACCAGAACA GCTTCTTTCA TTAGTGGAAC TTCAAATTCA TGAATGTCCG 
CAACTGCTTA TGGCTTCACT CACAGTTCCT ATAATTCGTC AGTTGAGGAT GGTGGATTTC 
GGTAAACTGC AGTTGCAAAT GCCGGGTTGT GACTTCACAG CTCTTCAAAC TTCAGAAATT 
GAAATTTTAG ATGTGTCTCA GTGGAGTCAA CTTCCAATGG CACCACACCA GCTCTCAATT 
AGAGAATGTG ATAATGCGGA GTCTCTGCTA GAGGAGGAAA TCTCGCAAAC CAACATACAT 
GATTGCAGTT TTTCTAGATC TCTGCACAAA GTTGGTTTAC CCACTACACT GAAATCACTA 
TTCATCTCTG AGTGCTCGAA ATTAGAGATT CTCGTACCTG AGTTGTCCAG ATGCCATCTC 
CCAGTCCTTG AAAGTCTAGA AATCAAAGGT GGTGTTATCG ATGATTCTCT CACGTTATCC 
TTCTCATTAG GCATCTTCCC CAAGTTGACT GATTTCACAA TCGATGGTCT TAAGGGGCTT 
GAGAAGCTCT CCATTTTGGT TTCAGAGGGT GATCCCACAT CTTTATGTTC CCTGCGTCTA 
ATTGGTTGCT CTGATCTTGA ATCTATCGAA TTGCACGCTC TCAACTTGGA GTCTTGTTTG 
ATTGATAGGT GCTTCAACCT TAGGTCGTTG GCACACACAC ACTCATATGT ACAGGAGTTG 
AAATTATGGG CTTGTCCAGA ATTGTTGTTT CAGAGAGAGG GTTTGCCTTC CAACCTACGT 
AAACTTGAAA TTGGCGAGTG CAACCAACTC ACGCCCCAGG TGGAGTGGGG TTTGCAAAGG 
CTGACCTCTC TTACTCATTT CACAATCACC GGTGGATGTG AAGACATTGA ATTATTTCCC 
AAGGAGTGTT TGCTGCCCTC TTCTCTGACT TCTCTTCAAA TAGAAATGCT TCCCAATCTC 
AAGTCTCTTG ACAGCGGGGG TCTTCAACAG CTCACCTCTC TAAAACGATT AGATATTTAT 
GGATGCTCGA GGCTCCAATC ATTGACAGAA GCGGGTCTTC AACACCTCAC CTCTCTTGAA 
ACGTTGTGGA TCGCACACTG CCCAGTGCTC CAATCATTGA CAGAAGCGGG TCTTCAACAC 
CTCACCTCTC TTGAAACGTT GTGGATCCTA GACTGCCCAG TGCTCCAATC ATTGACAGAA 
GCGGGTATTC AACACCTCAC CTCTCTTGAA ACGTTAGTGA TCGAAAACTG CCCAATGCTG 
CAATCCTTGA CAAAAGTGGG TCTTCAACAC CTCACCTCTC TTAAAGCATT GTATATCAAA 
AACTGTTGTA AGCTCAAATA CTTGACAAAA GAAAGACTTC CAGACTCCCT CTCTCTTCTG 
AGGATCTCCG AGTGTCCTTT ACTGGAAAAA CGGTGTCAAT TTGAGAAAGG GAAAGAATGG 
CGTTATATAG CTCACGTTCC AAAAATAATG ATCAATGGTG TGCTATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100853412 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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