F3H cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	F3H
Entrez Gene ID	100233079
Full Name	flavanone 3-hydroxylase
Synonyms	FHT
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281105.1	NP_001268034.1	naringenin,2-oxoglutarate 3-dioxygenase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

De novo transcriptome characterization of Vitis vinifera cv. Corvina unveils varietal diversity.
Venturini L, Ferrarini A, Zenoni S, Tornielli GB, Fasoli M, Dal Santo S, Minio A, Buson G, Tononi P, Zago ED, Zamperin G, Bellin D, Pezzotti M, Delledonne M
BMC genomics1441(2013 Jan)

Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves.
Gutha LR, Casassa LF, Harbertson JF, Naidu RA
BMC plant biology10187(2010 Aug)

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, Vezzulli S, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT, Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C, Davenport C, Demattè L, Mraz A, Battilana J, Stormo K, Costa F, Tao Q, Si-Ammour A, Harkins T, Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, Grando SM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P, Gutin A, Van de Peer Y, Salamini F, Viola R
PloS one2(12)e1326(2007 Dec)

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).
Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C
Plant molecular biology24(5)743-55(1994 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F3H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F3H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa00020	NM_001281105.1 Latest version!	Vitis vinifera flavanone 3-hydroxylase (F3H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F3H ( NM_001281105.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> NP_001268034.1 Vitis vinifera flavanone 3-hydroxylase (F3H), mRNA.

Price & Availability↑

CloneID	OVa00020
Clone ID Related Accession (Same CDS sequence)	NM_001281105.1
Accession Version	NM_001281105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Vitis vinifera(wine grape)
Product	naringenin,2-oxoglutarate 3-dioxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF192467.1. On Jul 24, 2013 this sequence version replaced XM_002267604.2. ##Evidence-Data-START## Transcript exon combination :: EF192467.1, FQ378052.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3598469, SAMEA3598470 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCCTA CGACACTGAC GGCTCTTGCA GGCGAGAAGA CTCTTCAGTC GAGCTTCGTC 
CGTGACGAAG ATGAACGTCC CAAGGTGGCC TACAACGACT TCAGCAACGA AATTCCTGTC 
ATCTCTCTCG AAGGAATCGA TGAAGTTGGC GGCCGTCGTG ATGAGATTTG CAGGAAAATC 
GTGGAGGCCT GCGAGGACTG GGGGATTTTC CAAGTTGTTA ATCACGGTGT TGATTCTAAT 
CTCATTTCGG AGATGACTCG CCTGGCTCGG GAATTCTTCG CTCTTCCGCC GGAGGAGAAG 
CTCCGCTTCG ACATGTCTGG TGGCAAGAAA GGCGGCTTCA TCGTTTCCAG CCATCTTCAA 
GGAGAAGCAG TGCAAGACTG GCGCGAGATC GTAACCTACT TCTCCTACCC ACTACGAACC 
AGGGACTACT CGAGGTGGCC AGACAAGCCA GAGGGGTGGA GATCGGTGAC ACAGGAGTAC 
AGCGAGAAGC TCATGGGGTT GGCCTGCAAG TTGCTGGAGG TGTTGTCTGA GGCCATGGAT 
TTGGACAAGG ACGCTTTGAC CAATGCTTGT GTGGACATGG ACCAGAAGGT TGTGGTCAAT 
TTCTACCCCC AATGCCCACA GCCAGACCTC ACCCTCGGAC TCAAGCGGCA TACCGATCCG 
GGAACCATCA CGCTGCTCCT TCAGGATCAG GTGGGAGGGC TCCAGGCCAC TAGGGATGGC 
GGAAAGACTT GGATCACCGT TCAACCTGTT GAAGGAGCTT TCGTCGTCAA CCTTGGCGAC 
CACGGTCATT ATCTGAGCAA TGGGAGGTTC AAGAATGCTG ATCACCAAGC GGTGGTGAAC 
TCCAATCATA GCAGACTGTC CATAGCAACA TTCCAAAACC CCGCACCGGA GGCAACCGTG 
TATCCTCTGA AGATAAGAGA GGGAGAGAAG GCAGTGCTTG AGGGACCCAT CACCTTTGCA 
GAGATGTACA GGAGGAAGAT GAGCAAAGAT CTGGAGCTTG CTAGGCTCAA GAAGTTGGCC 
AAGGAGCAGC AGTTGCAAGA CGTGGAGAAG GCCAAGTTAG AGTCCAAGCC CATTGACCAG 
ATTTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268034.1
CDS	25..1116
Translation	MAPTTLTALAGEKTLQSSFVRDEDERPKVAYNDFSNEIPVISLEGIDEVGGRRDEICRKIVEACEDWGIFQVVNHGVDSNLISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPLRTRDYSRWPDKPEGWRSVTQEYSEKLMGLACKLLEVLSEAMDLDKDALTNACVDMDQKVVVNFYPQCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNHSRLSIATFQNPAPEATVYPLKIREGEKAVLEGPITFAEMYRRKMSKDLELARLKKLAKEQQLQDVEKAKLESKPIDQILA

Target ORF information:

RefSeq Version	NM_001281105.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera flavanone 3-hydroxylase (F3H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281105.1

ORF Insert Sequence:

ATGGCGCCTA CGACACTGAC GGCTCTTGCA GGCGAGAAGA CTCTTCAGTC GAGCTTCGTC 
CGTGACGAAG ATGAACGTCC CAAGGTGGCC TACAACGACT TCAGCAACGA AATTCCTGTC 
ATCTCTCTCG AAGGAATCGA TGAAGTTGGC GGCCGTCGTG ATGAGATTTG CAGGAAAATC 
GTGGAGGCCT GCGAGGACTG GGGGATTTTC CAAGTTGTTA ATCACGGTGT TGATTCTAAT 
CTCATTTCGG AGATGACTCG CCTGGCTCGG GAATTCTTCG CTCTTCCGCC GGAGGAGAAG 
CTCCGCTTCG ACATGTCTGG TGGCAAGAAA GGCGGCTTCA TCGTTTCCAG CCATCTTCAA 
GGAGAAGCAG TGCAAGACTG GCGCGAGATC GTAACCTACT TCTCCTACCC ACTACGAACC 
AGGGACTACT CGAGGTGGCC AGACAAGCCA GAGGGGTGGA GATCGGTGAC ACAGGAGTAC 
AGCGAGAAGC TCATGGGGTT GGCCTGCAAG TTGCTGGAGG TGTTGTCTGA GGCCATGGAT 
TTGGACAAGG ACGCTTTGAC CAATGCTTGT GTGGACATGG ACCAGAAGGT TGTGGTCAAT 
TTCTACCCCC AATGCCCACA GCCAGACCTC ACCCTCGGAC TCAAGCGGCA TACCGATCCG 
GGAACCATCA CGCTGCTCCT TCAGGATCAG GTGGGAGGGC TCCAGGCCAC TAGGGATGGC 
GGAAAGACTT GGATCACCGT TCAACCTGTT GAAGGAGCTT TCGTCGTCAA CCTTGGCGAC 
CACGGTCATT ATCTGAGCAA TGGGAGGTTC AAGAATGCTG ATCACCAAGC GGTGGTGAAC 
TCCAATCATA GCAGACTGTC CATAGCAACA TTCCAAAACC CCGCACCGGA GGCAACCGTG 
TATCCTCTGA AGATAAGAGA GGGAGAGAAG GCAGTGCTTG AGGGACCCAT CACCTTTGCA 
GAGATGTACA GGAGGAAGAT GAGCAAAGAT CTGGAGCTTG CTAGGCTCAA GAAGTTGGCC 
AAGGAGCAGC AGTTGCAAGA CGTGGAGAAG GCCAAGTTAG AGTCCAAGCC CATTGACCAG 
ATTTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De novo transcriptome characterization of Vitis vinifera cv. Corvina unveils varietal diversity.
BMC genomics1441(2013 Jan)
Venturini L,Ferrarini A,Zenoni S,Tornielli GB,Fasoli M,Dal Santo S,Minio A,Buson G,Tononi P,Zago ED,Zamperin G,Bellin D,Pezzotti M,Delledonne M

Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves.
BMC plant biology10187(2010 Aug)
Gutha LR,Casassa LF,Harbertson JF,Naidu RA

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
PloS one2(12)e1326(2007 Dec)
Velasco R,Zharkikh A,Troggio M,Cartwright DA,Cestaro A,Pruss D,Pindo M,Fitzgerald LM,Vezzulli S,Reid J,Malacarne G,Iliev D,Coppola G,Wardell B,Micheletti D,Macalma T,Facci M,Mitchell JT,Perazzolli M,Eldredge G,Gatto P,Oyzerski R,Moretto M,Gutin N,Stefanini M,Chen Y,Segala C,Davenport C,Demattè L,Mraz A,Battilana J,Stormo K,Costa F,Tao Q,Si-Ammour A,Harkins T,Lackey A,Perbost C,Taillon B,Stella A,Solovyev V,Fawcett JA,Sterck L,Vandepoele K,Grando SM,Toppo S,Moser C,Lanchbury J,Bogden R,Skolnick M,Sgaramella V,Bhatnagar SK,Fontana P,Gutin A,Van de Peer Y,Salamini F,Viola R

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).
Plant molecular biology24(5)743-55(1994 Mar)
Sparvoli F,Martin C,Scienza A,Gavazzi G,Tonelli C

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F3H cDNA ORF clone, Vitis vinifera(wine grape)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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