N cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	N
Entrez Gene ID	661022
Full Name	Notch
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114381.1	NP_001107853.1	Notch
XM_015984186.1	XP_015839672.1	notch isoform X1
XM_008202082.2	XP_008200304.1	notch isoform X2

Pathways from BioSystems
Gene Ontology

The following N gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg15979	NM_001114381.1 Latest version!	Tribolium castaneum Notch (N), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTg14437	XM_008202082.2 Latest version!	Tribolium castaneum Notch (N), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTg22176	XM_015984186.1 Latest version!	Tribolium castaneum Notch (N), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N ( NM_001114381.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001107853.1 Tribolium castaneum Notch (N), mRNA.

Price & Availability↑

CloneID	OTg15979
Clone ID Related Accession (Same CDS sequence)	NM_001114381.1
Accession Version	NM_001114381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1535644800000
Organism	Tribolium castaneum(red flour beetle)
Product	Notch
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAJJ02004109.1. On Feb 9, 2008 this sequence version replaced XM_967212.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1523770, SAMEA1523771 [ECO:0000348] ##Evidence-Data-END##

ATGTGTTACA AAAACCACCA ATTTTTCAGC TGTACGTCGA AATACGTGGG TGAATACTGC 
CAGCATCTCA ACCCTTGTCA CACTGGTCCA GGTCCCCGAT GTCAAAACGG CGGTTCATGC 
ACTGTCCGAA TATCAGCCGG GGGTTCACCA AGTTTCACAT GCGCCTGTCC GTTGGGATTT 
AGTGCTTCGT TATGCGAAAT ACCAATCGCA AATTCGTGCG ATAAAAAGCC CTGTTTGAAT 
GGGGGTACTT GTTCGCTGAG ATCGTTAAAA GCGTATCAGT GCTCCTGTGC ACCGGGCTAT 
ACAGGAAAAC ACTGTGAAAA GGAGGACCAT TGCGCTTCGC AACCTTGCCG AAACGGCGCA 
AAATGCACAT CAGTGGGCGA TTCGTTTGAA TGCACGTGCG CTCCGGGATT CACCGGTCCT 
GCATGCAAGG ACGACAAGGA CGAATGCCGT CATAAGCCGT GCGTACATGG CAAATGTCAT 
AATACGCACG GCAGTTATAC GTGTACCTGT GACGAAGGCT ATACGGGACA AAACTGCGAA 
AGCGAGTACA TACCATGTGA CCCGAGTCCG TGCCTGAACG GCGGACAATG TCGCCAACGT 
GACAAACATA CATATACCTG TGATTGTCCT ACAGGTTTTC GTGGAACCAA TTGTGAAGAG 
AACATTGATG ATTGTCCAGG TCATCAATGT CGAAACGGTG CAACTTGTAT CGATGGCATC 
AATTCGTACA CGTGCCAATG TCCAGCAACG TATACTGGAC GTTTTTGTAC ACAAGACGTA 
GACGAGTGTT CATTAAGACC ATCAGTGTGT CAAAATGGTG CCACGTGTAC GAATAGTATT 
GGTGGTTTTT CATGTATTTG TGTTAACGGT TGGACTGGTG CTGATTGTTC TGTCAATATC 
GATGATTGTG CAGGAGCGGC GTGTTTCAAC GGTGCTACCT GTATTGATAG AGTTGGGAGT 
TTCTACTGCA GGTGTACGCC GGGAAAAACT GGTTTGTTGT GTCATCTGGA CGATGCCTGT 
ACAAGTAATC CCTGTCATGC GGATGCAATT TGTGACACAA GTCCAATAAA TGGGTCATAT 
ACTTGTAGCT GTGCATCAGG ATATAAAGGA ATCGATTGTT CAGAAGATAT AGACGAGTGC 
GAACAAGGTT CGCCATGCGA ACACGACGGA ATCTGCGTTA ACACCCCCGG CAGTTTCGCA 
TGCAATTGCA CGCAAGGCTT CACTGGACCA CGTTGTGAGA CCAATGTAAA TGAATGCGAA 
TCGCATCCAT GCCACAATGA TGGCTCCTGT TTGGATGATC CGGGAACATT TCGTTGCGTT 
TGTATGCCAG GTTTCACCGG CACCCAGTGC GAAATTGACA TCGACGAATG CAAAGATCAG 
CCCTGCCTTA ACGGCGGTGT CTGTCATGAC CTCATCAATT CGTTCAAATG TAGTTGTGCA 
ATCGGTTTCA CCGGTTCCCG TTGTCAAATC AATATCGACG ATTGTGTTTC ATCGCCTTGT 
CGAAATGGTG GCACTTGTCA TGATTCAATT GCCGGTTACA CGTGCGAATG TCCGCCCGGT 
TTCACCGGTT TGTCATGCGA AACAAACATC AATGATTGTC AATCGTCGCC ATGCCAACAT 
GGCGAATGTC TCGATGGTGA AAATTCCTTC AGTTGCACGT GTCATCCCGG TTACACCGGT 
TACCTATGTC AATATCAAAT CAACGAATGC GAATCGAATC CGTGCCAATA TGGCGGCTTG 
TGTCAGGATT TAGTCAACGG TTATCAATGC CTGTGTAAAG CCGGGACTTC AGGACCGAAT 
TGCGAGATCA ACGTCAATGA ATGTTACAGT AACCCATGTC GGAATAATGC AAGATGTGTC 
GATGGAATTA ATAGTTATAA CTGCGAATGT ATTCCGGGAT TCACCGGCAA ACATTGCGAG 
AATGACATAG ATGAGTGCGC TTCAAACCCG TGCGCCAATG GGGGCGTTTG CATCGACATG 
ATAAACGGTT TTCGATGCGA ATGCCCACGT GGTTACTATG ACGCTCGTTG CCTTAGTGAC 
GTTGACGAAT GCAATTCAAA TCCATGCAAA CATGGCGGCA GTTGCGAGGA TGGCGTCAAT 
CAATTCATCT GTCATTGTCT ACCAGGTTAC AGTGGTAAAC AATGCGAAAT CAACATTGAT 
GAATGCGCGT CAAATCCCTG TCAACACGGT GGCATTTGTC ACGATCATCT CGCCTCCTAT 
ACTTGCGAAT GTTTACCCGG TTATACTGGC ATTAATTGTG AAACGAATAT CGATGATTGT 
GCGATTAATC CGTGCAAAAA TCGTGGCACG TGTATTGATC AAGTCAATGA TTATAAATGT 
GTGTGTGAAT TGCCGTTTAC TGGACGTAAG TGCGAAGATA AGTTGGATCC GTGTACACCG 
AACCGGTGTC GGCATAACGC GAAATGTACG CCAAGTTCGA ATTATAAGGA TTTCGCGTGT 
ACTTGTAGCG GGGGTTATAC GGGGCGGTTT TGCGATCAAG ACGTCGATGA ATGTGTGGTT 
TCGAAACCGT GCCGAAACGG CGCCACTTGT AAGAATACCA ATGGGTCGTA CCATTGCATT 
TGTGCGCTGG GTTATGAAGG AAAAGACTGC AGTATTAATA CGGATGATTG TGCTTCACAT 
CCGTGTCAAA ATGGCGCCAC TTGCCTGGAC GACATTGGCG ATTACACCTG TCTATGTGTG 
AACGGTTTCG AAGGTAAACA ATGCGACATT GACATCGACG AATGTTTGTC AAATCCGTGT 
CAAAACGGTG CAACTTGCAA CCAGTACGTC GATTCATACA CGTGCACATG TCCATTGGGT 
TTCTCCGGCA TTAATTGTCA AACGAACGAT GAAGATTGCA CGGAAACTAG TTGCATGAAT 
GGTGGTACAT GCATTGATGG TATAAATTCG TACACTTGTA CGTGTAAACC GGGCTTTACC 
GGCTCAAATT GCCAAAATCG TATCAATCTA TGTGACAGTT CGCCGTGTTT AAATGGCGCT 
ACATGTCAAG ACCATACCAC GCATTACACG TGTCATTGTC CCTATGGTTA CACGGGTAAA 
GATTGTGGGG AGTATGTTGA TTGGTGTTCG ACGAATCCAT GCGAAAATCA AGCGACTTGT 
GTACAAAACA AAAACCAATA TCAGTGTTTG TGCGGCGTTG GTTGGACGGG GAAGGTATGT 
GATGTGGAAA TGGTCAGTTG TAAAGATGCA GCACTGCGTA AAGTTGTACC ATTGAAAAAA 
TTATGTAATA ATGGGACATG TGAGGACATT GGGAATTCCC ACCGTTGCCA TTGTTCGGAT 
GGTTATAGTG GTTCGTACTG TCAGACGGAA ATCAACGAAT GTGATTCGGC GCCGTGTCAA 
AACGGTGCCA CGTGTCTCGA TTTGATTGGT TCCTACTCGT GTAAATGCCC CAAAGGTTTC 
CAAGGCCAAA ATTGCGAACT AAACGTCGAT GATTGCAAAC CGAACCCGTG TCAAAACGGT 
GGCACTTGTC ACGATCTCGT TAATAGTTTT TCGTGTAGTT GTCCATCGGG TACTTTGGGT 
TACATTTGTG AGATTAATGT CGACGATTGT CGGCCAGGGG CGTGCCATAA CAACGGTACA 
TGTTTGGACA AAGTTGGCGG TTTTGAATGC AAATGCCCGC CTGGTTTTGT TGGCCCAAGA 
TGTGAAGGTG ACATTAATGA GTGTCTGTCA AATCCTTGCT CAAACGCCGG CACTCTAGAT 
TGCGTCCAGC TAGTAAACGA CTATCATTGT AACTGTAAAC TGGGCTTTAT GGGCCGACAT 
TGTGAAAGTA AAGTCAACTT TTGTGCGACT TCGCCGTGCC AAAACGGCGG TGTTTGTACA 
ACAATCCATG CTGGGCACAA ATGCACATGT CCTGAAGGTT TCTACGGAAA AAACTGCGAA 
TTCAGTGGTT ATGATTGTGA TTCGAATCCT TGTCAAAATG GTGGTGTTTG TCGCATCTCA 
GATGGCGGCG GTTACATTTG TGACTGTCCC AGTGGTACCA ATGGTACCAA CTGTGAAATT 
GACACGATCA ACGAATGTGA CAGTAACCCG TGTAAGCATT TGGACGCCAT TTGCCAGGAC 
AAACTGGGCG ATTATGCGTG TTATTGTCCC CCTAAACATA CGGGCAAAAA TTGTGAAATT 
TACGACAGGG GTTTCCCCGG TGGTCTGGGC CGGGCAGTGG TACCAACCCT TGACGCCACT 
AGTTACTATG CCAAGGATTT GGAAAAACAG CGGCAACAAT GTAACCAGAA CAAATGCCCA 
GTTAAGCGTG GTAACGGCCG CTGTGATGAG GAATGCAACA CTTATGCGTG CGAGTTTGAC 
GGAAATGATT GTTCCCTTGG GATTAATCCT TGGGCGAATT GCACGGCATC GACGAGGTGT 
TGGGAGGTTT TCATGGACGG GATTTGTAAC GAGGATTGTA ATAATCCCGA GTGTTTGTTT 
GACGGACGTG ATTGTGAAAA ATCGCTACAG CCGTGTAACC CCATTTACGA CGCCTACTGT 
CAGAAACACT ACGCTAATGG GTTGTGTGAT TATGGGTGTA ATAACGCTGA GTGTAACTGG 
GATGGGTTGG ACTGTGAAAA GGCACCGCCT GAGTTAGCCG AGGGTGTCAT TTCCATGATA 
ATCCTAATGG ACATGCAAGG TTTTCGCAAG AATTTGGTGG CATTTTTACG CGACACTAGC 
CACCAGTTAC GCACAAATGT CCGAGTGAAG AAGGATCAAG AAGGCCGCGA TATGATTTAC 
CCTTGGTTGG GGTCAGAACC TAGCAATTTT GACAGTTACT ACACGCGTAA ACACCACGTC 
AAATACACCG AACAAACGCA AAGCGGTGTC ATAGTCTACT TGGAGATTGA CAATCGGAAA 
TGTACAATAA CTGACAGTAG TGACAGTTGT TTTCAAACGG CAAGTGCCGC TGCTGAATTT 
CTGGCCGCGA AAGCGTCAAA ACACACACTG AGCCAATCGT TCCCCATTTA TCAAGTCAAC 
GGTTCTAGTG GAATAATTGA CGATCTGGAC ACGCCCACTA ATGCGAAATA CGTGGTCCTT 
GGTTTTATAA TCGTGTTTGC GTTCAGCGCT GCCATTGCTG TCATAGTAAC CACGCAACGG 
AAACGCGCCA GTGGCATCAC GTGGTTTCCG GAAGGCTGGT CATTGGCCAA CACTGGGCCG 
CGTAGGCGGA GCCGCAGACG TGGCCCCGAA GGACAAGAAC TGCGCAATAT GGGCAAACAC 
CCATCGGTCC CACTCGAAAT TGATAATCAT GGGCAAGGTC ATGTGCCACA GTGGTCCGAC 
GATGACTCAG ATATGCCACA ACCGAAGCGT TTCCGTGGCT TGGAGACGGG TTATGCGAGT 
GATCATACGG GGATTACCGA TTACGAGGAG GCGGAGCCAA GGGTGTGGTC ACAACAGCAT 
TTGGAGGCGG CTGATGTTAA ACCAATCTCG ATGCTGACTC CGCCCCAAGG CGATTTGAAT 
GATGTTAACG CACGTGGACC GTGCGGTATG ACTCCAATAA TGGTGGCGTC GACACGTGGC 
GGTGGCATGG ATACGGGTGA GGATGATGAG GATGATTCAG CGGGGGCGGT GATTCAGGAT 
TTGGTAGCGC AAGGGGCGGA GCTTAACGCT ACCATGGATA AAACAGGCGA GACGTCGTTA 
CATTTAGCGG CACGTTATGC CCGTGCCGAC GCCGCTAAAC GCCTACTTGA TGCCGGAGCG 
GATGCTAATG CGCAGGATAA TACGGGACGA ACGCCACTGC ATGCAGCTGT GGCGGCGGAT 
GCGTTGGGGG TTTTCCAGAT TTTGTTGCGG AATCGGGCGA CTAATCTCAA TGCAAGGATG 
CATGAAGGGA CGACGCCCTT GATCCTGGCA GCGAGACTAG CTATTGAAGG GATGGTTCAG 
GATTTGATTA GTGCTGAAGC TGATATTAAT GCGGCGGATA ATTCGGGGAA GACGGCATTG 
CATTGGGCCG CTGCTGTTAA TAATGTGGAG GCGGTTAATG TGTTGCTGGC CCATGGGGCC 
AATCGAGATG CACAAGATGA TAAGGATGAA ACGCCTCTGT TTCTTGCGGC AAGAGAAGGC 
TCATATCAGG CGTGTAAAGC ACTACTTGAA GCTTACGCTA ATCGGGAAAT TACCGACCAC 
ATGGATAGAT TGCCACGAGA TGTCGCCAGT GAACGTTTAC ATCATGATAT AGTGCGACTT 
TTGGATGAAC ATGTCCCAAG AAGTCCACAA ATGGTCAATG TTATACCCAA TAGTTCGATG 
ATGACATCAC CTAGCGGCCA ATTACAATTA CAACAACCAA CAGTAATCGT CCCAAAACGT 
CAAAAATCAA AAAGGCCGAG TAAAAACTCA AATAATAACA ATAACGCATC GTTGGACCCG 
ACAAGTCCCG AAATGGAAAC GATCAACACC GGAAGTATCC GACGTAAACC GAGCGTGAAA 
AAATCAAAGA AACCAACCCC ACAACAAGAA ATCCCCCAAA GTGTTGATTC AATAACTTCA 
ACCTTAAGTC CAGTTGAATC CCCAATGACG ACAAATCTAC CGAGTCCTTA CGACACTGCA 
TCGATTTACT CAAATATGGG CCTAACACCA CAAGGACTTG AGTCATTGAT TAATAAACAA 
CCACCAAGCT ATGAAGATGT GGTTAAGAAT AATTTACACG GTCTAGTTGG CTTGGAAAAT 
TACTCGTTCA ATTTGGGTAA TTTCCACGAT ACGATGATGA TGCAGCAGCG CCAATTACAA 
CAAGCCAACA CCTTAAGCCC GCCCTATTCG AATCAATCCC CGCCCCATTC CGTGCAATCG 
AATATGTCGT TATCGCCGCA AGGTTATAAC GGTTCACCAT CCCCGGCCAA GACACGCCCC 
TCGCTCCCGA CAAGCCCCAC CCATATAGCG GCAATGCGTG CCGCCACCCA ACAGAAGCAC 
GGAGGCGTGC CACAAGCACA AAATTTACAA ATGGGTTTTG ATTTTAGTCA AACTGGATTG 
GAGTTGCCAA TGGCGACATA TCCAGGATTG GTAAGTGGAA ATTTACAATT TCAACAACAA 
AACTATTATC TGACTCCGCC CTCACAACAC TCCGATCTCT TACCGGATAA TTTTCCAACA 
CCGAGTCCGG AAAGTCCGGG TCACTGGTCG TCGAGTTCAC CCCGGAGTTG TAATTCGGAT 
TGGTCCGAAA ATGTCGCCAG TCCGAATAAT ACTTATGGGG GTGGACATCA GACTAACAAA 
GGCTCGGATG CGATCTATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107853.1
CDS	1..7224
Translation	MCYKNHQFFSCTSKYVGEYCQHLNPCHTGPGPRCQNGGSCTVRISAGGSPSFTCACPLGFSASLCEIPIANSCDKKPCLNGGTCSLRSLKAYQCSCAPGYTGKHCEKEDHCASQPCRNGAKCTSVGDSFECTCAPGFTGPACKDDKDECRHKPCVHGKCHNTHGSYTCTCDEGYTGQNCESEYIPCDPSPCLNGGQCRQRDKHTYTCDCPTGFRGTNCEENIDDCPGHQCRNGATCIDGINSYTCQCPATYTGRFCTQDVDECSLRPSVCQNGATCTNSIGGFSCICVNGWTGADCSVNIDDCAGAACFNGATCIDRVGSFYCRCTPGKTGLLCHLDDACTSNPCHADAICDTSPINGSYTCSCASGYKGIDCSEDIDECEQGSPCEHDGICVNTPGSFACNCTQGFTGPRCETNVNECESHPCHNDGSCLDDPGTFRCVCMPGFTGTQCEIDIDECKDQPCLNGGVCHDLINSFKCSCAIGFTGSRCQINIDDCVSSPCRNGGTCHDSIAGYTCECPPGFTGLSCETNINDCQSSPCQHGECLDGENSFSCTCHPGYTGYLCQYQINECESNPCQYGGLCQDLVNGYQCLCKAGTSGPNCEINVNECYSNPCRNNARCVDGINSYNCECIPGFTGKHCENDIDECASNPCANGGVCIDMINGFRCECPRGYYDARCLSDVDECNSNPCKHGGSCEDGVNQFICHCLPGYSGKQCEINIDECASNPCQHGGICHDHLASYTCECLPGYTGINCETNIDDCAINPCKNRGTCIDQVNDYKCVCELPFTGRKCEDKLDPCTPNRCRHNAKCTPSSNYKDFACTCSGGYTGRFCDQDVDECVVSKPCRNGATCKNTNGSYHCICALGYEGKDCSINTDDCASHPCQNGATCLDDIGDYTCLCVNGFEGKQCDIDIDECLSNPCQNGATCNQYVDSYTCTCPLGFSGINCQTNDEDCTETSCMNGGTCIDGINSYTCTCKPGFTGSNCQNRINLCDSSPCLNGATCQDHTTHYTCHCPYGYTGKDCGEYVDWCSTNPCENQATCVQNKNQYQCLCGVGWTGKVCDVEMVSCKDAALRKVVPLKKLCNNGTCEDIGNSHRCHCSDGYSGSYCQTEINECDSAPCQNGATCLDLIGSYSCKCPKGFQGQNCELNVDDCKPNPCQNGGTCHDLVNSFSCSCPSGTLGYICEINVDDCRPGACHNNGTCLDKVGGFECKCPPGFVGPRCEGDINECLSNPCSNAGTLDCVQLVNDYHCNCKLGFMGRHCESKVNFCATSPCQNGGVCTTIHAGHKCTCPEGFYGKNCEFSGYDCDSNPCQNGGVCRISDGGGYICDCPSGTNGTNCEIDTINECDSNPCKHLDAICQDKLGDYACYCPPKHTGKNCEIYDRGFPGGLGRAVVPTLDATSYYAKDLEKQRQQCNQNKCPVKRGNGRCDEECNTYACEFDGNDCSLGINPWANCTASTRCWEVFMDGICNEDCNNPECLFDGRDCEKSLQPCNPIYDAYCQKHYANGLCDYGCNNAECNWDGLDCEKAPPELAEGVISMIILMDMQGFRKNLVAFLRDTSHQLRTNVRVKKDQEGRDMIYPWLGSEPSNFDSYYTRKHHVKYTEQTQSGVIVYLEIDNRKCTITDSSDSCFQTASAAAEFLAAKASKHTLSQSFPIYQVNGSSGIIDDLDTPTNAKYVVLGFIIVFAFSAAIAVIVTTQRKRASGITWFPEGWSLANTGPRRRSRRRGPEGQELRNMGKHPSVPLEIDNHGQGHVPQWSDDDSDMPQPKRFRGLETGYASDHTGITDYEEAEPRVWSQQHLEAADVKPISMLTPPQGDLNDVNARGPCGMTPIMVASTRGGGMDTGEDDEDDSAGAVIQDLVAQGAELNATMDKTGETSLHLAARYARADAAKRLLDAGADANAQDNTGRTPLHAAVAADALGVFQILLRNRATNLNARMHEGTTPLILAARLAIEGMVQDLISAEADINAADNSGKTALHWAAAVNNVEAVNVLLAHGANRDAQDDKDETPLFLAAREGSYQACKALLEAYANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMVNVIPNSSMMTSPSGQLQLQQPTVIVPKRQKSKRPSKNSNNNNNASLDPTSPEMETINTGSIRRKPSVKKSKKPTPQQEIPQSVDSITSTLSPVESPMTTNLPSPYDTASIYSNMGLTPQGLESLINKQPPSYEDVVKNNLHGLVGLENYSFNLGNFHDTMMMQQRQLQQANTLSPPYSNQSPPHSVQSNMSLSPQGYNGSPSPAKTRPSLPTSPTHIAAMRAATQQKHGGVPQAQNLQMGFDFSQTGLELPMATYPGLVSGNLQFQQQNYYLTPPSQHSDLLPDNFPTPSPESPGHWSSSSPRSCNSDWSENVASPNNTYGGGHQTNKGSDAIYI

Target ORF information:

RefSeq Version	NM_001114381.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Notch (N), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114381.1

ORF Insert Sequence:

ATGTGTTACA AAAACCACCA ATTTTTCAGC TGTACGTCGA AATACGTGGG TGAATACTGC 
CAGCATCTCA ACCCTTGTCA CACTGGTCCA GGTCCCCGAT GTCAAAACGG CGGTTCATGC 
ACTGTCCGAA TATCAGCCGG GGGTTCACCA AGTTTCACAT GCGCCTGTCC GTTGGGATTT 
AGTGCTTCGT TATGCGAAAT ACCAATCGCA AATTCGTGCG ATAAAAAGCC CTGTTTGAAT 
GGGGGTACTT GTTCGCTGAG ATCGTTAAAA GCGTATCAGT GCTCCTGTGC ACCGGGCTAT 
ACAGGAAAAC ACTGTGAAAA GGAGGACCAT TGCGCTTCGC AACCTTGCCG AAACGGCGCA 
AAATGCACAT CAGTGGGCGA TTCGTTTGAA TGCACGTGCG CTCCGGGATT CACCGGTCCT 
GCATGCAAGG ACGACAAGGA CGAATGCCGT CATAAGCCGT GCGTACATGG CAAATGTCAT 
AATACGCACG GCAGTTATAC GTGTACCTGT GACGAAGGCT ATACGGGACA AAACTGCGAA 
AGCGAGTACA TACCATGTGA CCCGAGTCCG TGCCTGAACG GCGGACAATG TCGCCAACGT 
GACAAACATA CATATACCTG TGATTGTCCT ACAGGTTTTC GTGGAACCAA TTGTGAAGAG 
AACATTGATG ATTGTCCAGG TCATCAATGT CGAAACGGTG CAACTTGTAT CGATGGCATC 
AATTCGTACA CGTGCCAATG TCCAGCAACG TATACTGGAC GTTTTTGTAC ACAAGACGTA 
GACGAGTGTT CATTAAGACC ATCAGTGTGT CAAAATGGTG CCACGTGTAC GAATAGTATT 
GGTGGTTTTT CATGTATTTG TGTTAACGGT TGGACTGGTG CTGATTGTTC TGTCAATATC 
GATGATTGTG CAGGAGCGGC GTGTTTCAAC GGTGCTACCT GTATTGATAG AGTTGGGAGT 
TTCTACTGCA GGTGTACGCC GGGAAAAACT GGTTTGTTGT GTCATCTGGA CGATGCCTGT 
ACAAGTAATC CCTGTCATGC GGATGCAATT TGTGACACAA GTCCAATAAA TGGGTCATAT 
ACTTGTAGCT GTGCATCAGG ATATAAAGGA ATCGATTGTT CAGAAGATAT AGACGAGTGC 
GAACAAGGTT CGCCATGCGA ACACGACGGA ATCTGCGTTA ACACCCCCGG CAGTTTCGCA 
TGCAATTGCA CGCAAGGCTT CACTGGACCA CGTTGTGAGA CCAATGTAAA TGAATGCGAA 
TCGCATCCAT GCCACAATGA TGGCTCCTGT TTGGATGATC CGGGAACATT TCGTTGCGTT 
TGTATGCCAG GTTTCACCGG CACCCAGTGC GAAATTGACA TCGACGAATG CAAAGATCAG 
CCCTGCCTTA ACGGCGGTGT CTGTCATGAC CTCATCAATT CGTTCAAATG TAGTTGTGCA 
ATCGGTTTCA CCGGTTCCCG TTGTCAAATC AATATCGACG ATTGTGTTTC ATCGCCTTGT 
CGAAATGGTG GCACTTGTCA TGATTCAATT GCCGGTTACA CGTGCGAATG TCCGCCCGGT 
TTCACCGGTT TGTCATGCGA AACAAACATC AATGATTGTC AATCGTCGCC ATGCCAACAT 
GGCGAATGTC TCGATGGTGA AAATTCCTTC AGTTGCACGT GTCATCCCGG TTACACCGGT 
TACCTATGTC AATATCAAAT CAACGAATGC GAATCGAATC CGTGCCAATA TGGCGGCTTG 
TGTCAGGATT TAGTCAACGG TTATCAATGC CTGTGTAAAG CCGGGACTTC AGGACCGAAT 
TGCGAGATCA ACGTCAATGA ATGTTACAGT AACCCATGTC GGAATAATGC AAGATGTGTC 
GATGGAATTA ATAGTTATAA CTGCGAATGT ATTCCGGGAT TCACCGGCAA ACATTGCGAG 
AATGACATAG ATGAGTGCGC TTCAAACCCG TGCGCCAATG GGGGCGTTTG CATCGACATG 
ATAAACGGTT TTCGATGCGA ATGCCCACGT GGTTACTATG ACGCTCGTTG CCTTAGTGAC 
GTTGACGAAT GCAATTCAAA TCCATGCAAA CATGGCGGCA GTTGCGAGGA TGGCGTCAAT 
CAATTCATCT GTCATTGTCT ACCAGGTTAC AGTGGTAAAC AATGCGAAAT CAACATTGAT 
GAATGCGCGT CAAATCCCTG TCAACACGGT GGCATTTGTC ACGATCATCT CGCCTCCTAT 
ACTTGCGAAT GTTTACCCGG TTATACTGGC ATTAATTGTG AAACGAATAT CGATGATTGT 
GCGATTAATC CGTGCAAAAA TCGTGGCACG TGTATTGATC AAGTCAATGA TTATAAATGT 
GTGTGTGAAT TGCCGTTTAC TGGACGTAAG TGCGAAGATA AGTTGGATCC GTGTACACCG 
AACCGGTGTC GGCATAACGC GAAATGTACG CCAAGTTCGA ATTATAAGGA TTTCGCGTGT 
ACTTGTAGCG GGGGTTATAC GGGGCGGTTT TGCGATCAAG ACGTCGATGA ATGTGTGGTT 
TCGAAACCGT GCCGAAACGG CGCCACTTGT AAGAATACCA ATGGGTCGTA CCATTGCATT 
TGTGCGCTGG GTTATGAAGG AAAAGACTGC AGTATTAATA CGGATGATTG TGCTTCACAT 
CCGTGTCAAA ATGGCGCCAC TTGCCTGGAC GACATTGGCG ATTACACCTG TCTATGTGTG 
AACGGTTTCG AAGGTAAACA ATGCGACATT GACATCGACG AATGTTTGTC AAATCCGTGT 
CAAAACGGTG CAACTTGCAA CCAGTACGTC GATTCATACA CGTGCACATG TCCATTGGGT 
TTCTCCGGCA TTAATTGTCA AACGAACGAT GAAGATTGCA CGGAAACTAG TTGCATGAAT 
GGTGGTACAT GCATTGATGG TATAAATTCG TACACTTGTA CGTGTAAACC GGGCTTTACC 
GGCTCAAATT GCCAAAATCG TATCAATCTA TGTGACAGTT CGCCGTGTTT AAATGGCGCT 
ACATGTCAAG ACCATACCAC GCATTACACG TGTCATTGTC CCTATGGTTA CACGGGTAAA 
GATTGTGGGG AGTATGTTGA TTGGTGTTCG ACGAATCCAT GCGAAAATCA AGCGACTTGT 
GTACAAAACA AAAACCAATA TCAGTGTTTG TGCGGCGTTG GTTGGACGGG GAAGGTATGT 
GATGTGGAAA TGGTCAGTTG TAAAGATGCA GCACTGCGTA AAGTTGTACC ATTGAAAAAA 
TTATGTAATA ATGGGACATG TGAGGACATT GGGAATTCCC ACCGTTGCCA TTGTTCGGAT 
GGTTATAGTG GTTCGTACTG TCAGACGGAA ATCAACGAAT GTGATTCGGC GCCGTGTCAA 
AACGGTGCCA CGTGTCTCGA TTTGATTGGT TCCTACTCGT GTAAATGCCC CAAAGGTTTC 
CAAGGCCAAA ATTGCGAACT AAACGTCGAT GATTGCAAAC CGAACCCGTG TCAAAACGGT 
GGCACTTGTC ACGATCTCGT TAATAGTTTT TCGTGTAGTT GTCCATCGGG TACTTTGGGT 
TACATTTGTG AGATTAATGT CGACGATTGT CGGCCAGGGG CGTGCCATAA CAACGGTACA 
TGTTTGGACA AAGTTGGCGG TTTTGAATGC AAATGCCCGC CTGGTTTTGT TGGCCCAAGA 
TGTGAAGGTG ACATTAATGA GTGTCTGTCA AATCCTTGCT CAAACGCCGG CACTCTAGAT 
TGCGTCCAGC TAGTAAACGA CTATCATTGT AACTGTAAAC TGGGCTTTAT GGGCCGACAT 
TGTGAAAGTA AAGTCAACTT TTGTGCGACT TCGCCGTGCC AAAACGGCGG TGTTTGTACA 
ACAATCCATG CTGGGCACAA ATGCACATGT CCTGAAGGTT TCTACGGAAA AAACTGCGAA 
TTCAGTGGTT ATGATTGTGA TTCGAATCCT TGTCAAAATG GTGGTGTTTG TCGCATCTCA 
GATGGCGGCG GTTACATTTG TGACTGTCCC AGTGGTACCA ATGGTACCAA CTGTGAAATT 
GACACGATCA ACGAATGTGA CAGTAACCCG TGTAAGCATT TGGACGCCAT TTGCCAGGAC 
AAACTGGGCG ATTATGCGTG TTATTGTCCC CCTAAACATA CGGGCAAAAA TTGTGAAATT 
TACGACAGGG GTTTCCCCGG TGGTCTGGGC CGGGCAGTGG TACCAACCCT TGACGCCACT 
AGTTACTATG CCAAGGATTT GGAAAAACAG CGGCAACAAT GTAACCAGAA CAAATGCCCA 
GTTAAGCGTG GTAACGGCCG CTGTGATGAG GAATGCAACA CTTATGCGTG CGAGTTTGAC 
GGAAATGATT GTTCCCTTGG GATTAATCCT TGGGCGAATT GCACGGCATC GACGAGGTGT 
TGGGAGGTTT TCATGGACGG GATTTGTAAC GAGGATTGTA ATAATCCCGA GTGTTTGTTT 
GACGGACGTG ATTGTGAAAA ATCGCTACAG CCGTGTAACC CCATTTACGA CGCCTACTGT 
CAGAAACACT ACGCTAATGG GTTGTGTGAT TATGGGTGTA ATAACGCTGA GTGTAACTGG 
GATGGGTTGG ACTGTGAAAA GGCACCGCCT GAGTTAGCCG AGGGTGTCAT TTCCATGATA 
ATCCTAATGG ACATGCAAGG TTTTCGCAAG AATTTGGTGG CATTTTTACG CGACACTAGC 
CACCAGTTAC GCACAAATGT CCGAGTGAAG AAGGATCAAG AAGGCCGCGA TATGATTTAC 
CCTTGGTTGG GGTCAGAACC TAGCAATTTT GACAGTTACT ACACGCGTAA ACACCACGTC 
AAATACACCG AACAAACGCA AAGCGGTGTC ATAGTCTACT TGGAGATTGA CAATCGGAAA 
TGTACAATAA CTGACAGTAG TGACAGTTGT TTTCAAACGG CAAGTGCCGC TGCTGAATTT 
CTGGCCGCGA AAGCGTCAAA ACACACACTG AGCCAATCGT TCCCCATTTA TCAAGTCAAC 
GGTTCTAGTG GAATAATTGA CGATCTGGAC ACGCCCACTA ATGCGAAATA CGTGGTCCTT 
GGTTTTATAA TCGTGTTTGC GTTCAGCGCT GCCATTGCTG TCATAGTAAC CACGCAACGG 
AAACGCGCCA GTGGCATCAC GTGGTTTCCG GAAGGCTGGT CATTGGCCAA CACTGGGCCG 
CGTAGGCGGA GCCGCAGACG TGGCCCCGAA GGACAAGAAC TGCGCAATAT GGGCAAACAC 
CCATCGGTCC CACTCGAAAT TGATAATCAT GGGCAAGGTC ATGTGCCACA GTGGTCCGAC 
GATGACTCAG ATATGCCACA ACCGAAGCGT TTCCGTGGCT TGGAGACGGG TTATGCGAGT 
GATCATACGG GGATTACCGA TTACGAGGAG GCGGAGCCAA GGGTGTGGTC ACAACAGCAT 
TTGGAGGCGG CTGATGTTAA ACCAATCTCG ATGCTGACTC CGCCCCAAGG CGATTTGAAT 
GATGTTAACG CACGTGGACC GTGCGGTATG ACTCCAATAA TGGTGGCGTC GACACGTGGC 
GGTGGCATGG ATACGGGTGA GGATGATGAG GATGATTCAG CGGGGGCGGT GATTCAGGAT 
TTGGTAGCGC AAGGGGCGGA GCTTAACGCT ACCATGGATA AAACAGGCGA GACGTCGTTA 
CATTTAGCGG CACGTTATGC CCGTGCCGAC GCCGCTAAAC GCCTACTTGA TGCCGGAGCG 
GATGCTAATG CGCAGGATAA TACGGGACGA ACGCCACTGC ATGCAGCTGT GGCGGCGGAT 
GCGTTGGGGG TTTTCCAGAT TTTGTTGCGG AATCGGGCGA CTAATCTCAA TGCAAGGATG 
CATGAAGGGA CGACGCCCTT GATCCTGGCA GCGAGACTAG CTATTGAAGG GATGGTTCAG 
GATTTGATTA GTGCTGAAGC TGATATTAAT GCGGCGGATA ATTCGGGGAA GACGGCATTG 
CATTGGGCCG CTGCTGTTAA TAATGTGGAG GCGGTTAATG TGTTGCTGGC CCATGGGGCC 
AATCGAGATG CACAAGATGA TAAGGATGAA ACGCCTCTGT TTCTTGCGGC AAGAGAAGGC 
TCATATCAGG CGTGTAAAGC ACTACTTGAA GCTTACGCTA ATCGGGAAAT TACCGACCAC 
ATGGATAGAT TGCCACGAGA TGTCGCCAGT GAACGTTTAC ATCATGATAT AGTGCGACTT 
TTGGATGAAC ATGTCCCAAG AAGTCCACAA ATGGTCAATG TTATACCCAA TAGTTCGATG 
ATGACATCAC CTAGCGGCCA ATTACAATTA CAACAACCAA CAGTAATCGT CCCAAAACGT 
CAAAAATCAA AAAGGCCGAG TAAAAACTCA AATAATAACA ATAACGCATC GTTGGACCCG 
ACAAGTCCCG AAATGGAAAC GATCAACACC GGAAGTATCC GACGTAAACC GAGCGTGAAA 
AAATCAAAGA AACCAACCCC ACAACAAGAA ATCCCCCAAA GTGTTGATTC AATAACTTCA 
ACCTTAAGTC CAGTTGAATC CCCAATGACG ACAAATCTAC CGAGTCCTTA CGACACTGCA 
TCGATTTACT CAAATATGGG CCTAACACCA CAAGGACTTG AGTCATTGAT TAATAAACAA 
CCACCAAGCT ATGAAGATGT GGTTAAGAAT AATTTACACG GTCTAGTTGG CTTGGAAAAT 
TACTCGTTCA ATTTGGGTAA TTTCCACGAT ACGATGATGA TGCAGCAGCG CCAATTACAA 
CAAGCCAACA CCTTAAGCCC GCCCTATTCG AATCAATCCC CGCCCCATTC CGTGCAATCG 
AATATGTCGT TATCGCCGCA AGGTTATAAC GGTTCACCAT CCCCGGCCAA GACACGCCCC 
TCGCTCCCGA CAAGCCCCAC CCATATAGCG GCAATGCGTG CCGCCACCCA ACAGAAGCAC 
GGAGGCGTGC CACAAGCACA AAATTTACAA ATGGGTTTTG ATTTTAGTCA AACTGGATTG 
GAGTTGCCAA TGGCGACATA TCCAGGATTG GTAAGTGGAA ATTTACAATT TCAACAACAA 
AACTATTATC TGACTCCGCC CTCACAACAC TCCGATCTCT TACCGGATAA TTTTCCAACA 
CCGAGTCCGG AAAGTCCGGG TCACTGGTCG TCGAGTTCAC CCCGGAGTTG TAATTCGGAT 
TGGTCCGAAA ATGTCGCCAG TCCGAATAAT ACTTATGGGG GTGGACATCA GACTAACAAA 
GGCTCGGATG CGATCTATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N ( XM_008202082.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008200304.1 Tribolium castaneum Notch (N), transcript variant X2, mRNA.

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CloneID	OTg14437
Clone ID Related Accession (Same CDS sequence)	XM_008202082.2
Accession Version	XM_008202082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	notch isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450205.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008202082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTA CTAACTGGAA GGATTATGAT TTACGGATTA TAGTGTCTTC CGCGGTTTTA 
CTGGCCCTAG CGAATGTAGC AACAGCTTTC GTTTCCTGCT CCCCTAGTCC ATGCAAAAAC 
GGAGGGACGT GTGTATCGTC GCTGAAAGGA GGCGATTCCT ATTGCAACTG TACGTCGAAA 
TACGTGGGTG AATACTGCCA GCATCTCAAC CCTTGTCACA CTGGTCCAGG TCCCCGATGT 
CAAAACGGCG GTTCATGCAC TGTCCGAATA TCAGCCGGGG GTTCACCAAG TTTCACATGC 
GCCTGTCCGT TGGGATTTAG TGCTTCGTTA TGCGAAATAC CAATCGCAAA TTCGTGCGAT 
AAAAAGCCCT GTTTGAATGG GGGTACTTGT TCGCTGAGAT CGTTAAAAGC GTATCAGTGC 
TCCTGTGCAC CGGGCTATAC AGGAAAACAC TGTGAAAAGG AGGACCATTG CGCTTCGCAA 
CCTTGCCGAA ACGGCGCAAA ATGCACATCA GTGGGCGATT CGTTTGAATG CACGTGCGCT 
CCGGGATTCA CCGGTCCTGC ATGCAAGGAC GACAAGGACG AATGCCGTCA TAAGCCGTGC 
GTACATGGCA AATGTCATAA TACGCACGGC AGTTATACGT GTACCTGTGA CGAAGGCTAT 
ACGGGACAAA ACTGCGAAAG CGAGTACATA CCATGTGACC CGAGTCCGTG CCTGAACGGC 
GGACAATGTC GCCAACGTGA CAAACATACA TATACCTGTG ATTGTCCTAC AGGTTTTCGT 
GGAACCAATT GTGAAGAGAA CATTGATGAT TGTCCAGGTC ATCAATGTCG AAACGGTGCA 
ACTTGTATCG ATGGCATCAA TTCGTACACG TGCCAATGTC CAGCAACGTA TACTGGACGT 
TTTTGTACAC AAGACGTAGA CGAGTGTTCA TTAAGACCAT CAGTGTGTCA AAATGGTGCC 
ACGTGTACGA ATAGTATTGG TGGTTTTTCA TGTATTTGTG TTAACGGTTG GACTGGTGCT 
GATTGTTCTG TCAATATCGA TGATTGTGCA GGAGCGGCGT GTTTCAACGG TGCTACCTGT 
ATTGATAGAG TTGGGAGTTT CTACTGCAGG TGTACGCCGG GAAAAACTGG TTTGTTGTGT 
CATCTGGACG ATGCCTGTAC AAGTAATCCC TGTCATGCGG ATGCAATTTG TGACACAAGT 
CCAATAAATG GGTCATATAC TTGTAGCTGT GCATCAGGAT ATAAAGGAAT CGATTGTTCA 
GAAGATATAG ACGAGTGCGA ACAAGGTTCG CCATGCGAAC ACGACGGAAT CTGCGTTAAC 
ACCCCCGGCA GTTTCGCATG CAATTGCACG CAAGGCTTCA CTGGACCACG TTGTGAGACC 
AATGTAAATG AATGCGAATC GCATCCATGC CACAATGATG GCTCCTGTTT GGATGATCCG 
GGAACATTTC GTTGCGTTTG TATGCCAGGT TTCACCGGCA CCCAGTGCGA AATTGACATC 
GACGAATGCA AAGATCAGCC CTGCCTTAAC GGCGGTGTCT GTCATGACCT CATCAATTCG 
TTCAAATGTA GTTGTGCAAT CGGTTTCACC GGTTCCCGTT GTCAAATCAA TATCGACGAT 
TGTGTTTCAT CGCCTTGTCG AAATGGTGGC ACTTGTCATG ATTCAATTGC CGGTTACACG 
TGCGAATGTC CGCCCGGTTT CACCGGTTTG TCATGCGAAA CAAACATCAA TGATTGTCAA 
TCGTCGCCAT GCCAACATGG CGAATGTCTC GATGGTGAAA ATTCCTTCAG TTGCACGTGT 
CATCCCGGTT ACACCGGTTA CCTATGTCAA TATCAAATCA ACGAATGCGA ATCGAATCCG 
TGCCAATATG GCGGCTTGTG TCAGGATTTA GTCAACGGTT ATCAATGCCT GTGTAAAGCC 
GGGACTTCAG GACCGAATTG CGAGATCAAC GTCAATGAAT GTTACAGTAA CCCATGTCGG 
AATAATGCAA GATGTGTCGA TGGAATTAAT AGTTATAACT GCGAATGTAT TCCGGGATTC 
ACCGGCAAAC ATTGCGAGAA TGACATAGAT GAGTGCGCTT CAAACCCGTG CGCCAATGGG 
GGCGTTTGCA TCGACATGAT AAACGGTTTT CGATGCGAAT GCCCACGTGG TTACTATGAC 
GCTCGTTGCC TTAGTGACGT TGACGAATGC AATTCAAATC CATGCAAACA TGGCGGCAGT 
TGCGAGGATG GCGTCAATCA ATTCATCTGT CATTGTCTAC CAGGTTACAG TGGTAAACAA 
TGCGAAATCA ACATTGATGA ATGCGCGTCA AATCCCTGTC AACACGGTGG CATTTGTCAC 
GATCATCTCG CCTCCTATAC TTGCGAATGT TTACCCGGTT ATACTGGCAT TAATTGTGAA 
ACGAATATCG ATGATTGTGC GATTAATCCG TGCAAAAATC GTGGCACGTG TATTGATCAA 
GTCAATGATT ATAAATGTGT GTGTGAATTG CCGTTTACTG GACGTAAGTG CGAAGATAAG 
TTGGATCCGT GTACACCGAA CCGGTGTCGG CATAACGCGA AATGTACGCC AAGTTCGAAT 
TATAAGGATT TCGCGTGTAC TTGTAGCGGG GGTTATACGG GGCGGTTTTG CGATCAAGAC 
GTCGATGAAT GTGTGGTTTC GAAACCGTGC CGAAACGGCG CCACTTGTAA GAATACCAAT 
GGGTCGTACC ATTGCATTTG TGCGCTGGGT TATGAAGGAA AAGACTGCAG TATTAATACG 
GATGATTGTG CTTCACATCC GTGTCAAAAT GGCGCCACTT GCCTGGACGA CATTGGCGAT 
TACACCTGTC TATGTGTGAA CGGTTTCGAA GGTAAACAAT GCGACATTGA CATCGACGAA 
TGTTTGTCAA ATCCGTGTCA AAACGGTGCA ACTTGCAACC AGTACGTCGA TTCATACACG 
TGCACATGTC CATTGGGTTT CTCCGGCATT AATTGTCAAA CGAACGATGA AGATTGCACG 
GAAACTAGTT GCATGAATGG TGGTACATGC ATTGATGGTA TAAATTCGTA CACTTGTACG 
TGTAAACCGG GCTTTACCGG CTCAAATTGC CAAAATCGTA TCAATCTATG TGACAGTTCG 
CCGTGTTTAA ATGGCGCTAC ATGTCAAGAC CATACCACGC ATTACACGTG TCATTGTCCC 
TATGGTTACA CGGGTAAAGA TTGTGGGGAG TATGTTGATT GGTGTTCGAC GAATCCATGC 
GAAAATCAAG CGACTTGTGT ACAAAACAAA AACCAATATC AGTGTTTGTG CGGCGTTGGT 
TGGACGGGGA AGGTATGTGA TGTGGAAATG GTCAGTTGTA AAGATGCAGC ACTGCGTAAA 
GTTGTACCAT TGAAAAAATT ATGTAATAAT GGGACATGTG AGGACATTGG GAATTCCCAC 
CGTTGCCATT GTTCGGATGG TTATAGTGGT TCGTACTGTC AGACGGAAAT CAACGAATGT 
GATTCGGCGC CGTGTCAAAA CGGTGCCACG TGTCTCGATT TGATTGGTTC CTACTCGTGT 
AAATGCCCCA AAGGTTTCCA AGGCCAAAAT TGCGAACTAA ACGTCGATGA TTGCAAACCG 
AACCCGTGTC AAAACGGTGG CACTTGTCAC GATCTCGTTA ATAGTTTTTC GTGTAGTTGT 
CCATCGGGTA CTTTGGGTTA CATTTGTGAG ATTAATGTCG ACGATTGTCG GCCAGGGGCG 
TGCCATAACA ACGGTACATG TTTGGACAAA GTTGGCGGTT TTGAATGCAA ATGCCCGCCT 
GGTTTTGTTG GCCCAAGATG TGAAGGTGAC ATTAATGAGT GTCTGTCAAA TCCTTGCTCA 
AACGCCGGCA CTCTAGATTG CGTCCAGCTA GTAAACGACT ATCATTGTAA CTGTAAACTG 
GGCTTTATGG GCCGACATTG TGAAAGTAAA GTCAACTTTT GTGCGACTTC GCCGTGCCAA 
AACGGCGGTG TTTGTACAAC AATCCATGCT GGGCACAAAT GCACATGTCC TGAAGGTTTC 
TACGGAAAAA ACTGCGAATT CAGTGGTTAT GATTGTGATT CGAATCCTTG TCAAAATGGT 
GGTGTTTGTC GCATCTCAGA TGGCGGCGGT TACATTTGTG ACTGTCCCAG TGGTACCAAT 
GGTACCAACT GTGAAATTGA CACGATCAAC GAATGTGACA GTAACCCGTG TAAGCATTTG 
GACGCCATTT GCCAGGACAA ACTGGGCGAT TATGCGTGTT ATTGTCCCCC TAAACATACG 
GGCAAAAATT GTGAAATTTA CGACAGGGGT TTCCCCGGTG GTCTGGGCCG GGCAGTGGTA 
CCAACCCTTG ACGCCACTAG TTACTATGCC AAGGATTTGG AAAAACAGCG GCAACAATGT 
AACCAGAACA AATGCCCAGT TAAGCGTGGT AACGGCCGCT GTGATGAGGA ATGCAACACT 
TATGCGTGCG AGTTTGACGG AAATGATTGT TCCCTTGGGA TTAATCCTTG GGCGAATTGC 
ACGGCATCGA CGAGGTGTTG GGAGGTTTTC ATGGACGGGA TTTGTAACGA GGATTGTAAT 
AATCCCGAGT GTTTGTTTGA CGGACGTGAT TGTGAAAAAT CGCTACAGCC GTGTAACCCC 
ATTTACGACG CCTACTGTCA GAAACACTAC GCTAATGGGT TGTGTGATTA TGGGTGTAAT 
AACGCTGAGT GTAACTGGGA TGGGTTGGAC TGTGAAAAGG CACCGCCTGA GTTAGCCGAG 
GGTGTCATTT CCATGATAAT CCTAATGGAC ATGCAAGGTT TTCGCAAGAA TTTGGTGGCA 
TTTTTACGCG ACACTAGCCA CCAGTTACGC ACAAATGTCC GAGTGAAGAA GGATCAAGAA 
GGCCGCGATA TGATTTACCC TTGGTTGGGG TCAGAACCTA GCAATTTTGA CAGTTACTAC 
ACGCGTAAAC ACCACGTCAA ATACACCGAA CAAACGCAAA GCGGTGTCAT AGTCTACTTG 
GAGATTGACA ATCGGAAATG TACAATAACT GACAGTAGTG ACAGTTGTTT TCAAACGGCA 
AGTGCCGCTG CTGAATTTCT GGCCGCGAAA GCGTCAAAAC ACACACTGAG CCAATCGTTC 
CCCATTTATC AAGTCAACGG TTCTAGTGGA ATAATTGACG ATCTGGACAC GCCCACTAAT 
GCGAAATACG TGGTCCTTGG TTTTATAATC GTGTTTGCGT TCAGCGCTGC CATTGCTGTC 
ATAGTAACCA CGCAACGGAA ACGCGCCAGT GGCATCACGT GGTTTCCGGA AGGCTGGTCA 
TTGGCCAACA CTGGGCCGCG TAGGCGGAGC CGCAGACGTG GCCCCGAAGG ACAAGAACTG 
CGCAATATGG GCAAACACCC ATCGGTCCCA CTCGAAATTG ATAATCATGG GCAAGGTCAT 
GTGCCACAGT GGTCCGACGA TGACTCAGAT ATGCCACAAC CGAAGCGTTT CCGTGGCTTG 
GAGACGGGTT ATGCGAGTGA TCATACGGGG ATTACCGATT ACGAGGAGGC GGAGCCAAGG 
GTGTGGTCAC AACAGCATTT GGAGGCGGCT GATGTTAAAC CAATCTCGAT GCTGACTCCG 
CCCCAAGGCG ATTTGAATGA TGTTAACGCA CGTGGACCGT GCGGTATGAC TCCAATAATG 
GTGGCGTCGA CACGTGGCGG TGGCATGGAT ACGGGTGAGG ATGATGAGGA TGATTCAGCG 
GGGGCGGTGA TTCAGGATTT GGTAGCGCAA GGGGCGGAGC TTAACGCTAC CATGGATAAA 
ACAGGCGAGA CGTCGTTACA TTTAGCGGCA CGTTATGCCC GTGCCGACGC CGCTAAACGC 
CTACTTGATG CCGGAGCGGA TGCTAATGCG CAGGATAATA CGGGACGAAC GCCACTGCAT 
GCAGCTGTGG CGGCGGATGC GTTGGGGGTT TTCCAGATTT TGTTGCGGAA TCGGGCGACT 
AATCTCAATG CAAGGATGCA TGAAGGGACG ACGCCCTTGA TCCTGGCAGC GAGACTAGCT 
ATTGAAGGGA TGGTTCAGGA TTTGATTAGT GCTGAAGCTG ATATTAATGC GGCGGATAAT 
TCGGGGAAGA CGGCATTGCA TTGGGCCGCT GCTGTTAATA ATGTGGAGGC GGTTAATGTG 
TTGCTGGCCC ATGGGGCCAA TCGAGATGCA CAAGATGATA AGGATGAAAC GCCTCTGTTT 
CTTGCGGCAA GAGAAGGCTC ATATCAGGCG TGTAAAGCAC TACTTGAAGC TTACGCTAAT 
CGGGAAATTA CCGACCACAT GGATAGATTG CCACGAGATG TCGCCAGTGA ACGTTTACAT 
CATGATATAG TGCGACTTTT GGATGAACAT GTCCCAAGAA GTCCACAAAT GGTCAATGTT 
ATACCCAATA GTTCGATGAT GACATCACCT AGCGGCCAAT TACAATTACA ACAACCAACA 
GTAATCGTCC CAAAACGTCA AAAATCAAAA AGGCCGAGTA AAAACTCAAA TAATAACAAT 
AACGCATCGT TGGACCCGAC AAGTCCCGAA ATGGAAACGA TCAACACCGG AAGTATCCGA 
CGTAAACCGA GCGTGAAAAA ATCAAAGAAA CCAACCCCAC AACAAGAAAT CCCCCAAAGT 
GTTGATTCAA TAACTTCAAC CTTAAGTCCA GTTGAATCCC CAATGACGAC AAATCTACCG 
AGTCCTTACG ACACTGCATC GATTTACTCA AATATGGGCC TAACACCACA AGGACTTGAG 
TCATTGATTA ATAAACAACC ACCAAGCTAT GAAGATGTGG TTAAGAATAA TTTACACGGT 
CTAGTTGGCT TGGAAAATTA CTCGTTCAAT TTGGGTAATT TCCACGATAC GATGATGATG 
CAGCAGCGCC AATTACAACA AGCCAACACC TTAAGCCCGC CCTATTCGAA TCAATCCCCG 
CCCCATTCCG TGCAATCGAA TATGTCGTTA TCGCCGCAAG GTTATAACGG TTCACCATCC 
CCGGCCAAGA CACGCCCCTC GCTCCCGACA AGCCCCACCC ATATAGCGGC AATGCGTGCC 
GCCACCCAAC AGAAGCACGG AGGCGTGCCA CAAGCACAAA ATTTACAAAT GGGTTTTGAT 
TTTAGTCAAA CTGGATTGGA GTTGCCAATG GCGACATATC CAGGATTGGT AAGTGGAAAT 
TTACAATTTC AACAACAAAA CTATTATCTG ACTCCGCCCT CACAACACTC CGATCTCTTA 
CCGGATAATT TTCCAACACC GAGTCCGGAA AGTCCGGGTC ACTGGTCGTC GAGTTCACCC 
CGGAGTTGTA ATTCGGATTG GTCCGAAAAT GTCGCCAGTC CGAATAATAC TTATGGGGGT 
GGACATCAGA CTAACAAAGG CTCGGATGCG ATCTATATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008200304.1
CDS	176..7537
Translation	MWITNWKDYDLRIIVSSAVLLALANVATAFVSCSPSPCKNGGTCVSSLKGGDSYCNCTSKYVGEYCQHLNPCHTGPGPRCQNGGSCTVRISAGGSPSFTCACPLGFSASLCEIPIANSCDKKPCLNGGTCSLRSLKAYQCSCAPGYTGKHCEKEDHCASQPCRNGAKCTSVGDSFECTCAPGFTGPACKDDKDECRHKPCVHGKCHNTHGSYTCTCDEGYTGQNCESEYIPCDPSPCLNGGQCRQRDKHTYTCDCPTGFRGTNCEENIDDCPGHQCRNGATCIDGINSYTCQCPATYTGRFCTQDVDECSLRPSVCQNGATCTNSIGGFSCICVNGWTGADCSVNIDDCAGAACFNGATCIDRVGSFYCRCTPGKTGLLCHLDDACTSNPCHADAICDTSPINGSYTCSCASGYKGIDCSEDIDECEQGSPCEHDGICVNTPGSFACNCTQGFTGPRCETNVNECESHPCHNDGSCLDDPGTFRCVCMPGFTGTQCEIDIDECKDQPCLNGGVCHDLINSFKCSCAIGFTGSRCQINIDDCVSSPCRNGGTCHDSIAGYTCECPPGFTGLSCETNINDCQSSPCQHGECLDGENSFSCTCHPGYTGYLCQYQINECESNPCQYGGLCQDLVNGYQCLCKAGTSGPNCEINVNECYSNPCRNNARCVDGINSYNCECIPGFTGKHCENDIDECASNPCANGGVCIDMINGFRCECPRGYYDARCLSDVDECNSNPCKHGGSCEDGVNQFICHCLPGYSGKQCEINIDECASNPCQHGGICHDHLASYTCECLPGYTGINCETNIDDCAINPCKNRGTCIDQVNDYKCVCELPFTGRKCEDKLDPCTPNRCRHNAKCTPSSNYKDFACTCSGGYTGRFCDQDVDECVVSKPCRNGATCKNTNGSYHCICALGYEGKDCSINTDDCASHPCQNGATCLDDIGDYTCLCVNGFEGKQCDIDIDECLSNPCQNGATCNQYVDSYTCTCPLGFSGINCQTNDEDCTETSCMNGGTCIDGINSYTCTCKPGFTGSNCQNRINLCDSSPCLNGATCQDHTTHYTCHCPYGYTGKDCGEYVDWCSTNPCENQATCVQNKNQYQCLCGVGWTGKVCDVEMVSCKDAALRKVVPLKKLCNNGTCEDIGNSHRCHCSDGYSGSYCQTEINECDSAPCQNGATCLDLIGSYSCKCPKGFQGQNCELNVDDCKPNPCQNGGTCHDLVNSFSCSCPSGTLGYICEINVDDCRPGACHNNGTCLDKVGGFECKCPPGFVGPRCEGDINECLSNPCSNAGTLDCVQLVNDYHCNCKLGFMGRHCESKVNFCATSPCQNGGVCTTIHAGHKCTCPEGFYGKNCEFSGYDCDSNPCQNGGVCRISDGGGYICDCPSGTNGTNCEIDTINECDSNPCKHLDAICQDKLGDYACYCPPKHTGKNCEIYDRGFPGGLGRAVVPTLDATSYYAKDLEKQRQQCNQNKCPVKRGNGRCDEECNTYACEFDGNDCSLGINPWANCTASTRCWEVFMDGICNEDCNNPECLFDGRDCEKSLQPCNPIYDAYCQKHYANGLCDYGCNNAECNWDGLDCEKAPPELAEGVISMIILMDMQGFRKNLVAFLRDTSHQLRTNVRVKKDQEGRDMIYPWLGSEPSNFDSYYTRKHHVKYTEQTQSGVIVYLEIDNRKCTITDSSDSCFQTASAAAEFLAAKASKHTLSQSFPIYQVNGSSGIIDDLDTPTNAKYVVLGFIIVFAFSAAIAVIVTTQRKRASGITWFPEGWSLANTGPRRRSRRRGPEGQELRNMGKHPSVPLEIDNHGQGHVPQWSDDDSDMPQPKRFRGLETGYASDHTGITDYEEAEPRVWSQQHLEAADVKPISMLTPPQGDLNDVNARGPCGMTPIMVASTRGGGMDTGEDDEDDSAGAVIQDLVAQGAELNATMDKTGETSLHLAARYARADAAKRLLDAGADANAQDNTGRTPLHAAVAADALGVFQILLRNRATNLNARMHEGTTPLILAARLAIEGMVQDLISAEADINAADNSGKTALHWAAAVNNVEAVNVLLAHGANRDAQDDKDETPLFLAAREGSYQACKALLEAYANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMVNVIPNSSMMTSPSGQLQLQQPTVIVPKRQKSKRPSKNSNNNNNASLDPTSPEMETINTGSIRRKPSVKKSKKPTPQQEIPQSVDSITSTLSPVESPMTTNLPSPYDTASIYSNMGLTPQGLESLINKQPPSYEDVVKNNLHGLVGLENYSFNLGNFHDTMMMQQRQLQQANTLSPPYSNQSPPHSVQSNMSLSPQGYNGSPSPAKTRPSLPTSPTHIAAMRAATQQKHGGVPQAQNLQMGFDFSQTGLELPMATYPGLVSGNLQFQQQNYYLTPPSQHSDLLPDNFPTPSPESPGHWSSSSPRSCNSDWSENVASPNNTYGGGHQTNKGSDAIYI

Target ORF information:

RefSeq Version	XM_008202082.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Notch (N), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008202082.2

ORF Insert Sequence:

ATGTGGATTA CTAACTGGAA GGATTATGAT TTACGGATTA TAGTGTCTTC CGCGGTTTTA 
CTGGCCCTAG CGAATGTAGC AACAGCTTTC GTTTCCTGCT CCCCTAGTCC ATGCAAAAAC 
GGAGGGACGT GTGTATCGTC GCTGAAAGGA GGCGATTCCT ATTGCAACTG TACGTCGAAA 
TACGTGGGTG AATACTGCCA GCATCTCAAC CCTTGTCACA CTGGTCCAGG TCCCCGATGT 
CAAAACGGCG GTTCATGCAC TGTCCGAATA TCAGCCGGGG GTTCACCAAG TTTCACATGC 
GCCTGTCCGT TGGGATTTAG TGCTTCGTTA TGCGAAATAC CAATCGCAAA TTCGTGCGAT 
AAAAAGCCCT GTTTGAATGG GGGTACTTGT TCGCTGAGAT CGTTAAAAGC GTATCAGTGC 
TCCTGTGCAC CGGGCTATAC AGGAAAACAC TGTGAAAAGG AGGACCATTG CGCTTCGCAA 
CCTTGCCGAA ACGGCGCAAA ATGCACATCA GTGGGCGATT CGTTTGAATG CACGTGCGCT 
CCGGGATTCA CCGGTCCTGC ATGCAAGGAC GACAAGGACG AATGCCGTCA TAAGCCGTGC 
GTACATGGCA AATGTCATAA TACGCACGGC AGTTATACGT GTACCTGTGA CGAAGGCTAT 
ACGGGACAAA ACTGCGAAAG CGAGTACATA CCATGTGACC CGAGTCCGTG CCTGAACGGC 
GGACAATGTC GCCAACGTGA CAAACATACA TATACCTGTG ATTGTCCTAC AGGTTTTCGT 
GGAACCAATT GTGAAGAGAA CATTGATGAT TGTCCAGGTC ATCAATGTCG AAACGGTGCA 
ACTTGTATCG ATGGCATCAA TTCGTACACG TGCCAATGTC CAGCAACGTA TACTGGACGT 
TTTTGTACAC AAGACGTAGA CGAGTGTTCA TTAAGACCAT CAGTGTGTCA AAATGGTGCC 
ACGTGTACGA ATAGTATTGG TGGTTTTTCA TGTATTTGTG TTAACGGTTG GACTGGTGCT 
GATTGTTCTG TCAATATCGA TGATTGTGCA GGAGCGGCGT GTTTCAACGG TGCTACCTGT 
ATTGATAGAG TTGGGAGTTT CTACTGCAGG TGTACGCCGG GAAAAACTGG TTTGTTGTGT 
CATCTGGACG ATGCCTGTAC AAGTAATCCC TGTCATGCGG ATGCAATTTG TGACACAAGT 
CCAATAAATG GGTCATATAC TTGTAGCTGT GCATCAGGAT ATAAAGGAAT CGATTGTTCA 
GAAGATATAG ACGAGTGCGA ACAAGGTTCG CCATGCGAAC ACGACGGAAT CTGCGTTAAC 
ACCCCCGGCA GTTTCGCATG CAATTGCACG CAAGGCTTCA CTGGACCACG TTGTGAGACC 
AATGTAAATG AATGCGAATC GCATCCATGC CACAATGATG GCTCCTGTTT GGATGATCCG 
GGAACATTTC GTTGCGTTTG TATGCCAGGT TTCACCGGCA CCCAGTGCGA AATTGACATC 
GACGAATGCA AAGATCAGCC CTGCCTTAAC GGCGGTGTCT GTCATGACCT CATCAATTCG 
TTCAAATGTA GTTGTGCAAT CGGTTTCACC GGTTCCCGTT GTCAAATCAA TATCGACGAT 
TGTGTTTCAT CGCCTTGTCG AAATGGTGGC ACTTGTCATG ATTCAATTGC CGGTTACACG 
TGCGAATGTC CGCCCGGTTT CACCGGTTTG TCATGCGAAA CAAACATCAA TGATTGTCAA 
TCGTCGCCAT GCCAACATGG CGAATGTCTC GATGGTGAAA ATTCCTTCAG TTGCACGTGT 
CATCCCGGTT ACACCGGTTA CCTATGTCAA TATCAAATCA ACGAATGCGA ATCGAATCCG 
TGCCAATATG GCGGCTTGTG TCAGGATTTA GTCAACGGTT ATCAATGCCT GTGTAAAGCC 
GGGACTTCAG GACCGAATTG CGAGATCAAC GTCAATGAAT GTTACAGTAA CCCATGTCGG 
AATAATGCAA GATGTGTCGA TGGAATTAAT AGTTATAACT GCGAATGTAT TCCGGGATTC 
ACCGGCAAAC ATTGCGAGAA TGACATAGAT GAGTGCGCTT CAAACCCGTG CGCCAATGGG 
GGCGTTTGCA TCGACATGAT AAACGGTTTT CGATGCGAAT GCCCACGTGG TTACTATGAC 
GCTCGTTGCC TTAGTGACGT TGACGAATGC AATTCAAATC CATGCAAACA TGGCGGCAGT 
TGCGAGGATG GCGTCAATCA ATTCATCTGT CATTGTCTAC CAGGTTACAG TGGTAAACAA 
TGCGAAATCA ACATTGATGA ATGCGCGTCA AATCCCTGTC AACACGGTGG CATTTGTCAC 
GATCATCTCG CCTCCTATAC TTGCGAATGT TTACCCGGTT ATACTGGCAT TAATTGTGAA 
ACGAATATCG ATGATTGTGC GATTAATCCG TGCAAAAATC GTGGCACGTG TATTGATCAA 
GTCAATGATT ATAAATGTGT GTGTGAATTG CCGTTTACTG GACGTAAGTG CGAAGATAAG 
TTGGATCCGT GTACACCGAA CCGGTGTCGG CATAACGCGA AATGTACGCC AAGTTCGAAT 
TATAAGGATT TCGCGTGTAC TTGTAGCGGG GGTTATACGG GGCGGTTTTG CGATCAAGAC 
GTCGATGAAT GTGTGGTTTC GAAACCGTGC CGAAACGGCG CCACTTGTAA GAATACCAAT 
GGGTCGTACC ATTGCATTTG TGCGCTGGGT TATGAAGGAA AAGACTGCAG TATTAATACG 
GATGATTGTG CTTCACATCC GTGTCAAAAT GGCGCCACTT GCCTGGACGA CATTGGCGAT 
TACACCTGTC TATGTGTGAA CGGTTTCGAA GGTAAACAAT GCGACATTGA CATCGACGAA 
TGTTTGTCAA ATCCGTGTCA AAACGGTGCA ACTTGCAACC AGTACGTCGA TTCATACACG 
TGCACATGTC CATTGGGTTT CTCCGGCATT AATTGTCAAA CGAACGATGA AGATTGCACG 
GAAACTAGTT GCATGAATGG TGGTACATGC ATTGATGGTA TAAATTCGTA CACTTGTACG 
TGTAAACCGG GCTTTACCGG CTCAAATTGC CAAAATCGTA TCAATCTATG TGACAGTTCG 
CCGTGTTTAA ATGGCGCTAC ATGTCAAGAC CATACCACGC ATTACACGTG TCATTGTCCC 
TATGGTTACA CGGGTAAAGA TTGTGGGGAG TATGTTGATT GGTGTTCGAC GAATCCATGC 
GAAAATCAAG CGACTTGTGT ACAAAACAAA AACCAATATC AGTGTTTGTG CGGCGTTGGT 
TGGACGGGGA AGGTATGTGA TGTGGAAATG GTCAGTTGTA AAGATGCAGC ACTGCGTAAA 
GTTGTACCAT TGAAAAAATT ATGTAATAAT GGGACATGTG AGGACATTGG GAATTCCCAC 
CGTTGCCATT GTTCGGATGG TTATAGTGGT TCGTACTGTC AGACGGAAAT CAACGAATGT 
GATTCGGCGC CGTGTCAAAA CGGTGCCACG TGTCTCGATT TGATTGGTTC CTACTCGTGT 
AAATGCCCCA AAGGTTTCCA AGGCCAAAAT TGCGAACTAA ACGTCGATGA TTGCAAACCG 
AACCCGTGTC AAAACGGTGG CACTTGTCAC GATCTCGTTA ATAGTTTTTC GTGTAGTTGT 
CCATCGGGTA CTTTGGGTTA CATTTGTGAG ATTAATGTCG ACGATTGTCG GCCAGGGGCG 
TGCCATAACA ACGGTACATG TTTGGACAAA GTTGGCGGTT TTGAATGCAA ATGCCCGCCT 
GGTTTTGTTG GCCCAAGATG TGAAGGTGAC ATTAATGAGT GTCTGTCAAA TCCTTGCTCA 
AACGCCGGCA CTCTAGATTG CGTCCAGCTA GTAAACGACT ATCATTGTAA CTGTAAACTG 
GGCTTTATGG GCCGACATTG TGAAAGTAAA GTCAACTTTT GTGCGACTTC GCCGTGCCAA 
AACGGCGGTG TTTGTACAAC AATCCATGCT GGGCACAAAT GCACATGTCC TGAAGGTTTC 
TACGGAAAAA ACTGCGAATT CAGTGGTTAT GATTGTGATT CGAATCCTTG TCAAAATGGT 
GGTGTTTGTC GCATCTCAGA TGGCGGCGGT TACATTTGTG ACTGTCCCAG TGGTACCAAT 
GGTACCAACT GTGAAATTGA CACGATCAAC GAATGTGACA GTAACCCGTG TAAGCATTTG 
GACGCCATTT GCCAGGACAA ACTGGGCGAT TATGCGTGTT ATTGTCCCCC TAAACATACG 
GGCAAAAATT GTGAAATTTA CGACAGGGGT TTCCCCGGTG GTCTGGGCCG GGCAGTGGTA 
CCAACCCTTG ACGCCACTAG TTACTATGCC AAGGATTTGG AAAAACAGCG GCAACAATGT 
AACCAGAACA AATGCCCAGT TAAGCGTGGT AACGGCCGCT GTGATGAGGA ATGCAACACT 
TATGCGTGCG AGTTTGACGG AAATGATTGT TCCCTTGGGA TTAATCCTTG GGCGAATTGC 
ACGGCATCGA CGAGGTGTTG GGAGGTTTTC ATGGACGGGA TTTGTAACGA GGATTGTAAT 
AATCCCGAGT GTTTGTTTGA CGGACGTGAT TGTGAAAAAT CGCTACAGCC GTGTAACCCC 
ATTTACGACG CCTACTGTCA GAAACACTAC GCTAATGGGT TGTGTGATTA TGGGTGTAAT 
AACGCTGAGT GTAACTGGGA TGGGTTGGAC TGTGAAAAGG CACCGCCTGA GTTAGCCGAG 
GGTGTCATTT CCATGATAAT CCTAATGGAC ATGCAAGGTT TTCGCAAGAA TTTGGTGGCA 
TTTTTACGCG ACACTAGCCA CCAGTTACGC ACAAATGTCC GAGTGAAGAA GGATCAAGAA 
GGCCGCGATA TGATTTACCC TTGGTTGGGG TCAGAACCTA GCAATTTTGA CAGTTACTAC 
ACGCGTAAAC ACCACGTCAA ATACACCGAA CAAACGCAAA GCGGTGTCAT AGTCTACTTG 
GAGATTGACA ATCGGAAATG TACAATAACT GACAGTAGTG ACAGTTGTTT TCAAACGGCA 
AGTGCCGCTG CTGAATTTCT GGCCGCGAAA GCGTCAAAAC ACACACTGAG CCAATCGTTC 
CCCATTTATC AAGTCAACGG TTCTAGTGGA ATAATTGACG ATCTGGACAC GCCCACTAAT 
GCGAAATACG TGGTCCTTGG TTTTATAATC GTGTTTGCGT TCAGCGCTGC CATTGCTGTC 
ATAGTAACCA CGCAACGGAA ACGCGCCAGT GGCATCACGT GGTTTCCGGA AGGCTGGTCA 
TTGGCCAACA CTGGGCCGCG TAGGCGGAGC CGCAGACGTG GCCCCGAAGG ACAAGAACTG 
CGCAATATGG GCAAACACCC ATCGGTCCCA CTCGAAATTG ATAATCATGG GCAAGGTCAT 
GTGCCACAGT GGTCCGACGA TGACTCAGAT ATGCCACAAC CGAAGCGTTT CCGTGGCTTG 
GAGACGGGTT ATGCGAGTGA TCATACGGGG ATTACCGATT ACGAGGAGGC GGAGCCAAGG 
GTGTGGTCAC AACAGCATTT GGAGGCGGCT GATGTTAAAC CAATCTCGAT GCTGACTCCG 
CCCCAAGGCG ATTTGAATGA TGTTAACGCA CGTGGACCGT GCGGTATGAC TCCAATAATG 
GTGGCGTCGA CACGTGGCGG TGGCATGGAT ACGGGTGAGG ATGATGAGGA TGATTCAGCG 
GGGGCGGTGA TTCAGGATTT GGTAGCGCAA GGGGCGGAGC TTAACGCTAC CATGGATAAA 
ACAGGCGAGA CGTCGTTACA TTTAGCGGCA CGTTATGCCC GTGCCGACGC CGCTAAACGC 
CTACTTGATG CCGGAGCGGA TGCTAATGCG CAGGATAATA CGGGACGAAC GCCACTGCAT 
GCAGCTGTGG CGGCGGATGC GTTGGGGGTT TTCCAGATTT TGTTGCGGAA TCGGGCGACT 
AATCTCAATG CAAGGATGCA TGAAGGGACG ACGCCCTTGA TCCTGGCAGC GAGACTAGCT 
ATTGAAGGGA TGGTTCAGGA TTTGATTAGT GCTGAAGCTG ATATTAATGC GGCGGATAAT 
TCGGGGAAGA CGGCATTGCA TTGGGCCGCT GCTGTTAATA ATGTGGAGGC GGTTAATGTG 
TTGCTGGCCC ATGGGGCCAA TCGAGATGCA CAAGATGATA AGGATGAAAC GCCTCTGTTT 
CTTGCGGCAA GAGAAGGCTC ATATCAGGCG TGTAAAGCAC TACTTGAAGC TTACGCTAAT 
CGGGAAATTA CCGACCACAT GGATAGATTG CCACGAGATG TCGCCAGTGA ACGTTTACAT 
CATGATATAG TGCGACTTTT GGATGAACAT GTCCCAAGAA GTCCACAAAT GGTCAATGTT 
ATACCCAATA GTTCGATGAT GACATCACCT AGCGGCCAAT TACAATTACA ACAACCAACA 
GTAATCGTCC CAAAACGTCA AAAATCAAAA AGGCCGAGTA AAAACTCAAA TAATAACAAT 
AACGCATCGT TGGACCCGAC AAGTCCCGAA ATGGAAACGA TCAACACCGG AAGTATCCGA 
CGTAAACCGA GCGTGAAAAA ATCAAAGAAA CCAACCCCAC AACAAGAAAT CCCCCAAAGT 
GTTGATTCAA TAACTTCAAC CTTAAGTCCA GTTGAATCCC CAATGACGAC AAATCTACCG 
AGTCCTTACG ACACTGCATC GATTTACTCA AATATGGGCC TAACACCACA AGGACTTGAG 
TCATTGATTA ATAAACAACC ACCAAGCTAT GAAGATGTGG TTAAGAATAA TTTACACGGT 
CTAGTTGGCT TGGAAAATTA CTCGTTCAAT TTGGGTAATT TCCACGATAC GATGATGATG 
CAGCAGCGCC AATTACAACA AGCCAACACC TTAAGCCCGC CCTATTCGAA TCAATCCCCG 
CCCCATTCCG TGCAATCGAA TATGTCGTTA TCGCCGCAAG GTTATAACGG TTCACCATCC 
CCGGCCAAGA CACGCCCCTC GCTCCCGACA AGCCCCACCC ATATAGCGGC AATGCGTGCC 
GCCACCCAAC AGAAGCACGG AGGCGTGCCA CAAGCACAAA ATTTACAAAT GGGTTTTGAT 
TTTAGTCAAA CTGGATTGGA GTTGCCAATG GCGACATATC CAGGATTGGT AAGTGGAAAT 
TTACAATTTC AACAACAAAA CTATTATCTG ACTCCGCCCT CACAACACTC CGATCTCTTA 
CCGGATAATT TTCCAACACC GAGTCCGGAA AGTCCGGGTC ACTGGTCGTC GAGTTCACCC 
CGGAGTTGTA ATTCGGATTG GTCCGAAAAT GTCGCCAGTC CGAATAATAC TTATGGGGGT 
GGACATCAGA CTAACAAAGG CTCGGATGCG ATCTATATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N ( XM_015984186.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015839672.1 Tribolium castaneum Notch (N), transcript variant X1, mRNA.

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CloneID	OTg22176
Clone ID Related Accession (Same CDS sequence)	XM_015984186.1
Accession Version	XM_015984186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	notch isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450205.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTA CTAACTGGAA GGATTATGAT TTACGGATTA TAGTGTCTTC CGCGGTTTTA 
CTGGCCCTAG CGAATGTAGC AACAGCTTTC GTTTCCTGCT CCCCTAGTCC ATGCAAAAAC 
GGAGGGACGT GTGTATCGTC GCTGAAAGGA GGCGATTCCT ATTGCAACTG TACGTCGAAA 
TACGTGGGTG AATACTGCCA GCATCTCAAC CCTTGTCACA CTGGTCCAGG TCCCCGATGT 
CAAAACGGCG GTTCATGCAC TGTCCGAATA TCAGCCGGGG GTTCACCAAG TTTCACATGC 
GCCTGTCCGT TGGGATTTAG TGCTTCGTTA TGCGAAATAC CAATCGCAAA TTCGTGCGAT 
AAAAAGCCCT GTTTGAATGG GGGTACTTGT TCGCTGAGAT CGTTAAAAGC GTATCAGTGC 
TCCTGTGCAC CGGGCTATAC AGGAAAACAC TGTGAAAAGG AGGACCATTG CGCTTCGCAA 
CCTTGCCGAA ACGGCGCAAA ATGCACATCA GTGGGCGATT CGTTTGAATG CACGTGCGCT 
CCGGGATTCA CCGGTCCTGC ATGCAAGGAC GACAAGGACG AATGCCGTCA TAAGCCGTGC 
GTACATGGCA AATGTCATAA TACGCACGGC AGTTATACGT GTACCTGTGA CGAAGGCTAT 
ACGGGACAAA ACTGCGAAAG CGAGTACATA CCATGTGACC CGAGTCCGTG CCTGAACGGC 
GGACAATGTC GCCAACGTGA CAAACATACA TATACCTGTG ATTGTCCTAC AGGTTTTCGT 
GGAACCAATT GTGAAGAGAA CATTGATGAT TGTCCAGGTC ATCAATGTCG AAACGGTGCA 
ACTTGTATCG ATGGCATCAA TTCGTACACG TGCCAATGTC CAGCAACGTA TACTGGACGT 
TTTTGTACAC AAGACGTAGA CGAGTGTTCA TTAAGACCAT CAGTGTGTCA AAATGGTGCC 
ACGTGTACGA ATAGTATTGG TGGTTTTTCA TGTATTTGTG TTAACGGTTG GACTGGTGCT 
GATTGTTCTG TCAATATCGA TGATTGTGCA GGAGCGGCGT GTTTCAACGG TGCTACCTGT 
ATTGATAGAG TTGGGAGTTT CTACTGCAGG TGTACGCCGG GAAAAACTGG TTTGTTGTGT 
CATCTGGACG ATGCCTGTAC AAGTAATCCC TGTCATGCGG ATGCAATTTG TGACACAAGT 
CCAATAAATG GGTCATATAC TTGTAGCTGT GCATCAGGAT ATAAAGGAAT CGATTGTTCA 
GAAGATATAG ACGAGTGCGA ACAAGGTTCG CCATGCGAAC ACGACGGAAT CTGCGTTAAC 
ACCCCCGGCA GTTTCGCATG CAATTGCACG CAAGGCTTCA CTGGACCACG TTGTGAGACC 
AATGTAAATG AATGCGAATC GCATCCATGC CACAATGATG GCTCCTGTTT GGATGATCCG 
GGAACATTTC GTTGCGTTTG TATGCCAGGT TTCACCGGCA CCCAGTGCGA AATTGACATC 
GACGAATGCA AAGATCAGCC CTGCCTTAAC GGCGGTGTCT GTCATGACCT CATCAATTCG 
TTCAAATGTA GTTGTGCAAT CGGTTTCACC GGTTCCCGTT GTCAAATCAA TATCGACGAT 
TGTGTTTCAT CGCCTTGTCG AAATGGTGGC ACTTGTCATG ATTCAATTGC CGGTTACACG 
TGCGAATGTC CGCCCGGTTT CACCGGTTTG TCATGCGAAA CAAACATCAA TGATTGTCAA 
TCGTCGCCAT GCCAACATGG CGAATGTCTC GATGGTGAAA ATTCCTTCAG TTGCACGTGT 
CATCCCGGTT ACACCGGTTA CCTATGTCAA TATCAAATCA ACGAATGCGA ATCGAATCCG 
TGCCAATATG GCGGCTTGTG TCAGGATTTA GTCAACGGTT ATCAATGCCT GTGTAAAGCC 
GGGACTTCAG GACCGAATTG CGAGATCAAC GTCAATGAAT GTTACAGTAA CCCATGTCGG 
AATAATGCAA GATGTGTCGA TGGAATTAAT AGTAGGGATT ATAACTGCGA ATGTATTCCG 
GGATTCACCG GCAAACATTG CGAGAATGAC ATAGATGAGT GCGCTTCAAA CCCGTGCGCC 
AATGGGGGCG TTTGCATCGA CATGATAAAC GGTTTTCGAT GCGAATGCCC ACGTGGTTAC 
TATGACGCTC GTTGCCTTAG TGACGTTGAC GAATGCAATT CAAATCCATG CAAACATGGC 
GGCAGTTGCG AGGATGGCGT CAATCAATTC ATCTGTCATT GTCTACCAGG TTACAGTGGT 
AAACAATGCG AAATCAACAT TGATGAATGC GCGTCAAATC CCTGTCAACA CGGTGGCATT 
TGTCACGATC ATCTCGCCTC CTATACTTGC GAATGTTTAC CCGGTTATAC TGGCATTAAT 
TGTGAAACGA ATATCGATGA TTGTGCGATT AATCCGTGCA AAAATCGTGG CACGTGTATT 
GATCAAGTCA ATGATTATAA ATGTGTGTGT GAATTGCCGT TTACTGGACG TAAGTGCGAA 
GATAAGTTGG ATCCGTGTAC ACCGAACCGG TGTCGGCATA ACGCGAAATG TACGCCAAGT 
TCGAATTATA AGGATTTCGC GTGTACTTGT AGCGGGGGTT ATACGGGGCG GTTTTGCGAT 
CAAGACGTCG ATGAATGTGT GGTTTCGAAA CCGTGCCGAA ACGGCGCCAC TTGTAAGAAT 
ACCAATGGGT CGTACCATTG CATTTGTGCG CTGGGTTATG AAGGAAAAGA CTGCAGTATT 
AATACGGATG ATTGTGCTTC ACATCCGTGT CAAAATGGCG CCACTTGCCT GGACGACATT 
GGCGATTACA CCTGTCTATG TGTGAACGGT TTCGAAGGTA AACAATGCGA CATTGACATC 
GACGAATGTT TGTCAAATCC GTGTCAAAAC GGTGCAACTT GCAACCAGTA CGTCGATTCA 
TACACGTGCA CATGTCCATT GGGTTTCTCC GGCATTAATT GTCAAACGAA CGATGAAGAT 
TGCACGGAAA CTAGTTGCAT GAATGGTGGT ACATGCATTG ATGGTATAAA TTCGTACACT 
TGTACGTGTA AACCGGGCTT TACCGGCTCA AATTGCCAAA ATCGTATCAA TCTATGTGAC 
AGTTCGCCGT GTTTAAATGG CGCTACATGT CAAGACCATA CCACGCATTA CACGTGTCAT 
TGTCCCTATG GTTACACGGG TAAAGATTGT GGGGAGTATG TTGATTGGTG TTCGACGAAT 
CCATGCGAAA ATCAAGCGAC TTGTGTACAA AACAAAAACC AATATCAGTG TTTGTGCGGC 
GTTGGTTGGA CGGGGAAGGT ATGTGATGTG GAAATGGTCA GTTGTAAAGA TGCAGCACTG 
CGTAAAGTTG TACCATTGAA AAAATTATGT AATAATGGGA CATGTGAGGA CATTGGGAAT 
TCCCACCGTT GCCATTGTTC GGATGGTTAT AGTGGTTCGT ACTGTCAGAC GGAAATCAAC 
GAATGTGATT CGGCGCCGTG TCAAAACGGT GCCACGTGTC TCGATTTGAT TGGTTCCTAC 
TCGTGTAAAT GCCCCAAAGG TTTCCAAGGC CAAAATTGCG AACTAAACGT CGATGATTGC 
AAACCGAACC CGTGTCAAAA CGGTGGCACT TGTCACGATC TCGTTAATAG TTTTTCGTGT 
AGTTGTCCAT CGGGTACTTT GGGTTACATT TGTGAGATTA ATGTCGACGA TTGTCGGCCA 
GGGGCGTGCC ATAACAACGG TACATGTTTG GACAAAGTTG GCGGTTTTGA ATGCAAATGC 
CCGCCTGGTT TTGTTGGCCC AAGATGTGAA GGTGACATTA ATGAGTGTCT GTCAAATCCT 
TGCTCAAACG CCGGCACTCT AGATTGCGTC CAGCTAGTAA ACGACTATCA TTGTAACTGT 
AAACTGGGCT TTATGGGCCG ACATTGTGAA AGTAAAGTCA ACTTTTGTGC GACTTCGCCG 
TGCCAAAACG GCGGTGTTTG TACAACAATC CATGCTGGGC ACAAATGCAC ATGTCCTGAA 
GGTTTCTACG GAAAAAACTG CGAATTCAGT GGTTATGATT GTGATTCGAA TCCTTGTCAA 
AATGGTGGTG TTTGTCGCAT CTCAGATGGC GGCGGTTACA TTTGTGACTG TCCCAGTGGT 
ACCAATGGTA CCAACTGTGA AATTGACACG ATCAACGAAT GTGACAGTAA CCCGTGTAAG 
CATTTGGACG CCATTTGCCA GGACAAACTG GGCGATTATG CGTGTTATTG TCCCCCTAAA 
CATACGGGCA AAAATTGTGA AATTTACGAC AGGGGTTTCC CCGGTGGTCT GGGCCGGGCA 
GTGGTACCAA CCCTTGACGC CACTAGTTAC TATGCCAAGG ATTTGGAAAA ACAGCGGCAA 
CAATGTAACC AGAACAAATG CCCAGTTAAG CGTGGTAACG GCCGCTGTGA TGAGGAATGC 
AACACTTATG CGTGCGAGTT TGACGGAAAT GATTGTTCCC TTGGGATTAA TCCTTGGGCG 
AATTGCACGG CATCGACGAG GTGTTGGGAG GTTTTCATGG ACGGGATTTG TAACGAGGAT 
TGTAATAATC CCGAGTGTTT GTTTGACGGA CGTGATTGTG AAAAATCGCT ACAGCCGTGT 
AACCCCATTT ACGACGCCTA CTGTCAGAAA CACTACGCTA ATGGGTTGTG TGATTATGGG 
TGTAATAACG CTGAGTGTAA CTGGGATGGG TTGGACTGTG AAAAGGCACC GCCTGAGTTA 
GCCGAGGGTG TCATTTCCAT GATAATCCTA ATGGACATGC AAGGTTTTCG CAAGAATTTG 
GTGGCATTTT TACGCGACAC TAGCCACCAG TTACGCACAA ATGTCCGAGT GAAGAAGGAT 
CAAGAAGGCC GCGATATGAT TTACCCTTGG TTGGGGTCAG AACCTAGCAA TTTTGACAGT 
TACTACACGC GTAAACACCA CGTCAAATAC ACCGAACAAA CGCAAAGCGG TGTCATAGTC 
TACTTGGAGA TTGACAATCG GAAATGTACA ATAACTGACA GTAGTGACAG TTGTTTTCAA 
ACGGCAAGTG CCGCTGCTGA ATTTCTGGCC GCGAAAGCGT CAAAACACAC ACTGAGCCAA 
TCGTTCCCCA TTTATCAAGT CAACGGTTCT AGTGGAATAA TTGACGATCT GGACACGCCC 
ACTAATGCGA AATACGTGGT CCTTGGTTTT ATAATCGTGT TTGCGTTCAG CGCTGCCATT 
GCTGTCATAG TAACCACGCA ACGGAAACGC GCCAGTGGCA TCACGTGGTT TCCGGAAGGC 
TGGTCATTGG CCAACACTGG GCCGCGTAGG CGGAGCCGCA GACGTGGCCC CGAAGGACAA 
GAACTGCGCA ATATGGGCAA ACACCCATCG GTCCCACTCG AAATTGATAA TCATGGGCAA 
GGTCATGTGC CACAGTGGTC CGACGATGAC TCAGATATGC CACAACCGAA GCGTTTCCGT 
GGCTTGGAGA CGGGTTATGC GAGTGATCAT ACGGGGATTA CCGATTACGA GGAGGCGGAG 
CCAAGGGTGT GGTCACAACA GCATTTGGAG GCGGCTGATG TTAAACCAAT CTCGATGCTG 
ACTCCGCCCC AAGGCGATTT GAATGATGTT AACGCACGTG GACCGTGCGG TATGACTCCA 
ATAATGGTGG CGTCGACACG TGGCGGTGGC ATGGATACGG GTGAGGATGA TGAGGATGAT 
TCAGCGGGGG CGGTGATTCA GGATTTGGTA GCGCAAGGGG CGGAGCTTAA CGCTACCATG 
GATAAAACAG GCGAGACGTC GTTACATTTA GCGGCACGTT ATGCCCGTGC CGACGCCGCT 
AAACGCCTAC TTGATGCCGG AGCGGATGCT AATGCGCAGG ATAATACGGG ACGAACGCCA 
CTGCATGCAG CTGTGGCGGC GGATGCGTTG GGGGTTTTCC AGATTTTGTT GCGGAATCGG 
GCGACTAATC TCAATGCAAG GATGCATGAA GGGACGACGC CCTTGATCCT GGCAGCGAGA 
CTAGCTATTG AAGGGATGGT TCAGGATTTG ATTAGTGCTG AAGCTGATAT TAATGCGGCG 
GATAATTCGG GGAAGACGGC ATTGCATTGG GCCGCTGCTG TTAATAATGT GGAGGCGGTT 
AATGTGTTGC TGGCCCATGG GGCCAATCGA GATGCACAAG ATGATAAGGA TGAAACGCCT 
CTGTTTCTTG CGGCAAGAGA AGGCTCATAT CAGGCGTGTA AAGCACTACT TGAAGCTTAC 
GCTAATCGGG AAATTACCGA CCACATGGAT AGATTGCCAC GAGATGTCGC CAGTGAACGT 
TTACATCATG ATATAGTGCG ACTTTTGGAT GAACATGTCC CAAGAAGTCC ACAAATGGTC 
AATGTTATAC CCAATAGTTC GATGATGACA TCACCTAGCG GCCAATTACA ATTACAACAA 
CCAACAGTAA TCGTCCCAAA ACGTCAAAAA TCAAAAAGGC CGAGTAAAAA CTCAAATAAT 
AACAATAACG CATCGTTGGA CCCGACAAGT CCCGAAATGG AAACGATCAA CACCGGAAGT 
ATCCGACGTA AACCGAGCGT GAAAAAATCA AAGAAACCAA CCCCACAACA AGAAATCCCC 
CAAAGTGTTG ATTCAATAAC TTCAACCTTA AGTCCAGTTG AATCCCCAAT GACGACAAAT 
CTACCGAGTC CTTACGACAC TGCATCGATT TACTCAAATA TGGGCCTAAC ACCACAAGGA 
CTTGAGTCAT TGATTAATAA ACAACCACCA AGCTATGAAG ATGTGGTTAA GAATAATTTA 
CACGGTCTAG TTGGCTTGGA AAATTACTCG TTCAATTTGG GTAATTTCCA CGATACGATG 
ATGATGCAGC AGCGCCAATT ACAACAAGCC AACACCTTAA GCCCGCCCTA TTCGAATCAA 
TCCCCGCCCC ATTCCGTGCA ATCGAATATG TCGTTATCGC CGCAAGGTTA TAACGGTTCA 
CCATCCCCGG CCAAGACACG CCCCTCGCTC CCGACAAGCC CCACCCATAT AGCGGCAATG 
CGTGCCGCCA CCCAACAGAA GCACGGAGGC GTGCCACAAG CACAAAATTT ACAAATGGGT 
TTTGATTTTA GTCAAACTGG ATTGGAGTTG CCAATGGCGA CATATCCAGG ATTGGTAAGT 
GGAAATTTAC AATTTCAACA ACAAAACTAT TATCTGACTC CGCCCTCACA ACACTCCGAT 
CTCTTACCGG ATAATTTTCC AACACCGAGT CCGGAAAGTC CGGGTCACTG GTCGTCGAGT 
TCACCCCGGA GTTGTAATTC GGATTGGTCC GAAAATGTCG CCAGTCCGAA TAATACTTAT 
GGGGGTGGAC ATCAGACTAA CAAAGGCTCG GATGCGATCT ATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015839672.1
CDS	176..7543
Translation	MWITNWKDYDLRIIVSSAVLLALANVATAFVSCSPSPCKNGGTCVSSLKGGDSYCNCTSKYVGEYCQHLNPCHTGPGPRCQNGGSCTVRISAGGSPSFTCACPLGFSASLCEIPIANSCDKKPCLNGGTCSLRSLKAYQCSCAPGYTGKHCEKEDHCASQPCRNGAKCTSVGDSFECTCAPGFTGPACKDDKDECRHKPCVHGKCHNTHGSYTCTCDEGYTGQNCESEYIPCDPSPCLNGGQCRQRDKHTYTCDCPTGFRGTNCEENIDDCPGHQCRNGATCIDGINSYTCQCPATYTGRFCTQDVDECSLRPSVCQNGATCTNSIGGFSCICVNGWTGADCSVNIDDCAGAACFNGATCIDRVGSFYCRCTPGKTGLLCHLDDACTSNPCHADAICDTSPINGSYTCSCASGYKGIDCSEDIDECEQGSPCEHDGICVNTPGSFACNCTQGFTGPRCETNVNECESHPCHNDGSCLDDPGTFRCVCMPGFTGTQCEIDIDECKDQPCLNGGVCHDLINSFKCSCAIGFTGSRCQINIDDCVSSPCRNGGTCHDSIAGYTCECPPGFTGLSCETNINDCQSSPCQHGECLDGENSFSCTCHPGYTGYLCQYQINECESNPCQYGGLCQDLVNGYQCLCKAGTSGPNCEINVNECYSNPCRNNARCVDGINSRDYNCECIPGFTGKHCENDIDECASNPCANGGVCIDMINGFRCECPRGYYDARCLSDVDECNSNPCKHGGSCEDGVNQFICHCLPGYSGKQCEINIDECASNPCQHGGICHDHLASYTCECLPGYTGINCETNIDDCAINPCKNRGTCIDQVNDYKCVCELPFTGRKCEDKLDPCTPNRCRHNAKCTPSSNYKDFACTCSGGYTGRFCDQDVDECVVSKPCRNGATCKNTNGSYHCICALGYEGKDCSINTDDCASHPCQNGATCLDDIGDYTCLCVNGFEGKQCDIDIDECLSNPCQNGATCNQYVDSYTCTCPLGFSGINCQTNDEDCTETSCMNGGTCIDGINSYTCTCKPGFTGSNCQNRINLCDSSPCLNGATCQDHTTHYTCHCPYGYTGKDCGEYVDWCSTNPCENQATCVQNKNQYQCLCGVGWTGKVCDVEMVSCKDAALRKVVPLKKLCNNGTCEDIGNSHRCHCSDGYSGSYCQTEINECDSAPCQNGATCLDLIGSYSCKCPKGFQGQNCELNVDDCKPNPCQNGGTCHDLVNSFSCSCPSGTLGYICEINVDDCRPGACHNNGTCLDKVGGFECKCPPGFVGPRCEGDINECLSNPCSNAGTLDCVQLVNDYHCNCKLGFMGRHCESKVNFCATSPCQNGGVCTTIHAGHKCTCPEGFYGKNCEFSGYDCDSNPCQNGGVCRISDGGGYICDCPSGTNGTNCEIDTINECDSNPCKHLDAICQDKLGDYACYCPPKHTGKNCEIYDRGFPGGLGRAVVPTLDATSYYAKDLEKQRQQCNQNKCPVKRGNGRCDEECNTYACEFDGNDCSLGINPWANCTASTRCWEVFMDGICNEDCNNPECLFDGRDCEKSLQPCNPIYDAYCQKHYANGLCDYGCNNAECNWDGLDCEKAPPELAEGVISMIILMDMQGFRKNLVAFLRDTSHQLRTNVRVKKDQEGRDMIYPWLGSEPSNFDSYYTRKHHVKYTEQTQSGVIVYLEIDNRKCTITDSSDSCFQTASAAAEFLAAKASKHTLSQSFPIYQVNGSSGIIDDLDTPTNAKYVVLGFIIVFAFSAAIAVIVTTQRKRASGITWFPEGWSLANTGPRRRSRRRGPEGQELRNMGKHPSVPLEIDNHGQGHVPQWSDDDSDMPQPKRFRGLETGYASDHTGITDYEEAEPRVWSQQHLEAADVKPISMLTPPQGDLNDVNARGPCGMTPIMVASTRGGGMDTGEDDEDDSAGAVIQDLVAQGAELNATMDKTGETSLHLAARYARADAAKRLLDAGADANAQDNTGRTPLHAAVAADALGVFQILLRNRATNLNARMHEGTTPLILAARLAIEGMVQDLISAEADINAADNSGKTALHWAAAVNNVEAVNVLLAHGANRDAQDDKDETPLFLAAREGSYQACKALLEAYANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMVNVIPNSSMMTSPSGQLQLQQPTVIVPKRQKSKRPSKNSNNNNNASLDPTSPEMETINTGSIRRKPSVKKSKKPTPQQEIPQSVDSITSTLSPVESPMTTNLPSPYDTASIYSNMGLTPQGLESLINKQPPSYEDVVKNNLHGLVGLENYSFNLGNFHDTMMMQQRQLQQANTLSPPYSNQSPPHSVQSNMSLSPQGYNGSPSPAKTRPSLPTSPTHIAAMRAATQQKHGGVPQAQNLQMGFDFSQTGLELPMATYPGLVSGNLQFQQQNYYLTPPSQHSDLLPDNFPTPSPESPGHWSSSSPRSCNSDWSENVASPNNTYGGGHQTNKGSDAIYI

Target ORF information:

RefSeq Version	XM_015984186.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Notch (N), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015984186.1

ORF Insert Sequence:

ATGTGGATTA CTAACTGGAA GGATTATGAT TTACGGATTA TAGTGTCTTC CGCGGTTTTA 
CTGGCCCTAG CGAATGTAGC AACAGCTTTC GTTTCCTGCT CCCCTAGTCC ATGCAAAAAC 
GGAGGGACGT GTGTATCGTC GCTGAAAGGA GGCGATTCCT ATTGCAACTG TACGTCGAAA 
TACGTGGGTG AATACTGCCA GCATCTCAAC CCTTGTCACA CTGGTCCAGG TCCCCGATGT 
CAAAACGGCG GTTCATGCAC TGTCCGAATA TCAGCCGGGG GTTCACCAAG TTTCACATGC 
GCCTGTCCGT TGGGATTTAG TGCTTCGTTA TGCGAAATAC CAATCGCAAA TTCGTGCGAT 
AAAAAGCCCT GTTTGAATGG GGGTACTTGT TCGCTGAGAT CGTTAAAAGC GTATCAGTGC 
TCCTGTGCAC CGGGCTATAC AGGAAAACAC TGTGAAAAGG AGGACCATTG CGCTTCGCAA 
CCTTGCCGAA ACGGCGCAAA ATGCACATCA GTGGGCGATT CGTTTGAATG CACGTGCGCT 
CCGGGATTCA CCGGTCCTGC ATGCAAGGAC GACAAGGACG AATGCCGTCA TAAGCCGTGC 
GTACATGGCA AATGTCATAA TACGCACGGC AGTTATACGT GTACCTGTGA CGAAGGCTAT 
ACGGGACAAA ACTGCGAAAG CGAGTACATA CCATGTGACC CGAGTCCGTG CCTGAACGGC 
GGACAATGTC GCCAACGTGA CAAACATACA TATACCTGTG ATTGTCCTAC AGGTTTTCGT 
GGAACCAATT GTGAAGAGAA CATTGATGAT TGTCCAGGTC ATCAATGTCG AAACGGTGCA 
ACTTGTATCG ATGGCATCAA TTCGTACACG TGCCAATGTC CAGCAACGTA TACTGGACGT 
TTTTGTACAC AAGACGTAGA CGAGTGTTCA TTAAGACCAT CAGTGTGTCA AAATGGTGCC 
ACGTGTACGA ATAGTATTGG TGGTTTTTCA TGTATTTGTG TTAACGGTTG GACTGGTGCT 
GATTGTTCTG TCAATATCGA TGATTGTGCA GGAGCGGCGT GTTTCAACGG TGCTACCTGT 
ATTGATAGAG TTGGGAGTTT CTACTGCAGG TGTACGCCGG GAAAAACTGG TTTGTTGTGT 
CATCTGGACG ATGCCTGTAC AAGTAATCCC TGTCATGCGG ATGCAATTTG TGACACAAGT 
CCAATAAATG GGTCATATAC TTGTAGCTGT GCATCAGGAT ATAAAGGAAT CGATTGTTCA 
GAAGATATAG ACGAGTGCGA ACAAGGTTCG CCATGCGAAC ACGACGGAAT CTGCGTTAAC 
ACCCCCGGCA GTTTCGCATG CAATTGCACG CAAGGCTTCA CTGGACCACG TTGTGAGACC 
AATGTAAATG AATGCGAATC GCATCCATGC CACAATGATG GCTCCTGTTT GGATGATCCG 
GGAACATTTC GTTGCGTTTG TATGCCAGGT TTCACCGGCA CCCAGTGCGA AATTGACATC 
GACGAATGCA AAGATCAGCC CTGCCTTAAC GGCGGTGTCT GTCATGACCT CATCAATTCG 
TTCAAATGTA GTTGTGCAAT CGGTTTCACC GGTTCCCGTT GTCAAATCAA TATCGACGAT 
TGTGTTTCAT CGCCTTGTCG AAATGGTGGC ACTTGTCATG ATTCAATTGC CGGTTACACG 
TGCGAATGTC CGCCCGGTTT CACCGGTTTG TCATGCGAAA CAAACATCAA TGATTGTCAA 
TCGTCGCCAT GCCAACATGG CGAATGTCTC GATGGTGAAA ATTCCTTCAG TTGCACGTGT 
CATCCCGGTT ACACCGGTTA CCTATGTCAA TATCAAATCA ACGAATGCGA ATCGAATCCG 
TGCCAATATG GCGGCTTGTG TCAGGATTTA GTCAACGGTT ATCAATGCCT GTGTAAAGCC 
GGGACTTCAG GACCGAATTG CGAGATCAAC GTCAATGAAT GTTACAGTAA CCCATGTCGG 
AATAATGCAA GATGTGTCGA TGGAATTAAT AGTAGGGATT ATAACTGCGA ATGTATTCCG 
GGATTCACCG GCAAACATTG CGAGAATGAC ATAGATGAGT GCGCTTCAAA CCCGTGCGCC 
AATGGGGGCG TTTGCATCGA CATGATAAAC GGTTTTCGAT GCGAATGCCC ACGTGGTTAC 
TATGACGCTC GTTGCCTTAG TGACGTTGAC GAATGCAATT CAAATCCATG CAAACATGGC 
GGCAGTTGCG AGGATGGCGT CAATCAATTC ATCTGTCATT GTCTACCAGG TTACAGTGGT 
AAACAATGCG AAATCAACAT TGATGAATGC GCGTCAAATC CCTGTCAACA CGGTGGCATT 
TGTCACGATC ATCTCGCCTC CTATACTTGC GAATGTTTAC CCGGTTATAC TGGCATTAAT 
TGTGAAACGA ATATCGATGA TTGTGCGATT AATCCGTGCA AAAATCGTGG CACGTGTATT 
GATCAAGTCA ATGATTATAA ATGTGTGTGT GAATTGCCGT TTACTGGACG TAAGTGCGAA 
GATAAGTTGG ATCCGTGTAC ACCGAACCGG TGTCGGCATA ACGCGAAATG TACGCCAAGT 
TCGAATTATA AGGATTTCGC GTGTACTTGT AGCGGGGGTT ATACGGGGCG GTTTTGCGAT 
CAAGACGTCG ATGAATGTGT GGTTTCGAAA CCGTGCCGAA ACGGCGCCAC TTGTAAGAAT 
ACCAATGGGT CGTACCATTG CATTTGTGCG CTGGGTTATG AAGGAAAAGA CTGCAGTATT 
AATACGGATG ATTGTGCTTC ACATCCGTGT CAAAATGGCG CCACTTGCCT GGACGACATT 
GGCGATTACA CCTGTCTATG TGTGAACGGT TTCGAAGGTA AACAATGCGA CATTGACATC 
GACGAATGTT TGTCAAATCC GTGTCAAAAC GGTGCAACTT GCAACCAGTA CGTCGATTCA 
TACACGTGCA CATGTCCATT GGGTTTCTCC GGCATTAATT GTCAAACGAA CGATGAAGAT 
TGCACGGAAA CTAGTTGCAT GAATGGTGGT ACATGCATTG ATGGTATAAA TTCGTACACT 
TGTACGTGTA AACCGGGCTT TACCGGCTCA AATTGCCAAA ATCGTATCAA TCTATGTGAC 
AGTTCGCCGT GTTTAAATGG CGCTACATGT CAAGACCATA CCACGCATTA CACGTGTCAT 
TGTCCCTATG GTTACACGGG TAAAGATTGT GGGGAGTATG TTGATTGGTG TTCGACGAAT 
CCATGCGAAA ATCAAGCGAC TTGTGTACAA AACAAAAACC AATATCAGTG TTTGTGCGGC 
GTTGGTTGGA CGGGGAAGGT ATGTGATGTG GAAATGGTCA GTTGTAAAGA TGCAGCACTG 
CGTAAAGTTG TACCATTGAA AAAATTATGT AATAATGGGA CATGTGAGGA CATTGGGAAT 
TCCCACCGTT GCCATTGTTC GGATGGTTAT AGTGGTTCGT ACTGTCAGAC GGAAATCAAC 
GAATGTGATT CGGCGCCGTG TCAAAACGGT GCCACGTGTC TCGATTTGAT TGGTTCCTAC 
TCGTGTAAAT GCCCCAAAGG TTTCCAAGGC CAAAATTGCG AACTAAACGT CGATGATTGC 
AAACCGAACC CGTGTCAAAA CGGTGGCACT TGTCACGATC TCGTTAATAG TTTTTCGTGT 
AGTTGTCCAT CGGGTACTTT GGGTTACATT TGTGAGATTA ATGTCGACGA TTGTCGGCCA 
GGGGCGTGCC ATAACAACGG TACATGTTTG GACAAAGTTG GCGGTTTTGA ATGCAAATGC 
CCGCCTGGTT TTGTTGGCCC AAGATGTGAA GGTGACATTA ATGAGTGTCT GTCAAATCCT 
TGCTCAAACG CCGGCACTCT AGATTGCGTC CAGCTAGTAA ACGACTATCA TTGTAACTGT 
AAACTGGGCT TTATGGGCCG ACATTGTGAA AGTAAAGTCA ACTTTTGTGC GACTTCGCCG 
TGCCAAAACG GCGGTGTTTG TACAACAATC CATGCTGGGC ACAAATGCAC ATGTCCTGAA 
GGTTTCTACG GAAAAAACTG CGAATTCAGT GGTTATGATT GTGATTCGAA TCCTTGTCAA 
AATGGTGGTG TTTGTCGCAT CTCAGATGGC GGCGGTTACA TTTGTGACTG TCCCAGTGGT 
ACCAATGGTA CCAACTGTGA AATTGACACG ATCAACGAAT GTGACAGTAA CCCGTGTAAG 
CATTTGGACG CCATTTGCCA GGACAAACTG GGCGATTATG CGTGTTATTG TCCCCCTAAA 
CATACGGGCA AAAATTGTGA AATTTACGAC AGGGGTTTCC CCGGTGGTCT GGGCCGGGCA 
GTGGTACCAA CCCTTGACGC CACTAGTTAC TATGCCAAGG ATTTGGAAAA ACAGCGGCAA 
CAATGTAACC AGAACAAATG CCCAGTTAAG CGTGGTAACG GCCGCTGTGA TGAGGAATGC 
AACACTTATG CGTGCGAGTT TGACGGAAAT GATTGTTCCC TTGGGATTAA TCCTTGGGCG 
AATTGCACGG CATCGACGAG GTGTTGGGAG GTTTTCATGG ACGGGATTTG TAACGAGGAT 
TGTAATAATC CCGAGTGTTT GTTTGACGGA CGTGATTGTG AAAAATCGCT ACAGCCGTGT 
AACCCCATTT ACGACGCCTA CTGTCAGAAA CACTACGCTA ATGGGTTGTG TGATTATGGG 
TGTAATAACG CTGAGTGTAA CTGGGATGGG TTGGACTGTG AAAAGGCACC GCCTGAGTTA 
GCCGAGGGTG TCATTTCCAT GATAATCCTA ATGGACATGC AAGGTTTTCG CAAGAATTTG 
GTGGCATTTT TACGCGACAC TAGCCACCAG TTACGCACAA ATGTCCGAGT GAAGAAGGAT 
CAAGAAGGCC GCGATATGAT TTACCCTTGG TTGGGGTCAG AACCTAGCAA TTTTGACAGT 
TACTACACGC GTAAACACCA CGTCAAATAC ACCGAACAAA CGCAAAGCGG TGTCATAGTC 
TACTTGGAGA TTGACAATCG GAAATGTACA ATAACTGACA GTAGTGACAG TTGTTTTCAA 
ACGGCAAGTG CCGCTGCTGA ATTTCTGGCC GCGAAAGCGT CAAAACACAC ACTGAGCCAA 
TCGTTCCCCA TTTATCAAGT CAACGGTTCT AGTGGAATAA TTGACGATCT GGACACGCCC 
ACTAATGCGA AATACGTGGT CCTTGGTTTT ATAATCGTGT TTGCGTTCAG CGCTGCCATT 
GCTGTCATAG TAACCACGCA ACGGAAACGC GCCAGTGGCA TCACGTGGTT TCCGGAAGGC 
TGGTCATTGG CCAACACTGG GCCGCGTAGG CGGAGCCGCA GACGTGGCCC CGAAGGACAA 
GAACTGCGCA ATATGGGCAA ACACCCATCG GTCCCACTCG AAATTGATAA TCATGGGCAA 
GGTCATGTGC CACAGTGGTC CGACGATGAC TCAGATATGC CACAACCGAA GCGTTTCCGT 
GGCTTGGAGA CGGGTTATGC GAGTGATCAT ACGGGGATTA CCGATTACGA GGAGGCGGAG 
CCAAGGGTGT GGTCACAACA GCATTTGGAG GCGGCTGATG TTAAACCAAT CTCGATGCTG 
ACTCCGCCCC AAGGCGATTT GAATGATGTT AACGCACGTG GACCGTGCGG TATGACTCCA 
ATAATGGTGG CGTCGACACG TGGCGGTGGC ATGGATACGG GTGAGGATGA TGAGGATGAT 
TCAGCGGGGG CGGTGATTCA GGATTTGGTA GCGCAAGGGG CGGAGCTTAA CGCTACCATG 
GATAAAACAG GCGAGACGTC GTTACATTTA GCGGCACGTT ATGCCCGTGC CGACGCCGCT 
AAACGCCTAC TTGATGCCGG AGCGGATGCT AATGCGCAGG ATAATACGGG ACGAACGCCA 
CTGCATGCAG CTGTGGCGGC GGATGCGTTG GGGGTTTTCC AGATTTTGTT GCGGAATCGG 
GCGACTAATC TCAATGCAAG GATGCATGAA GGGACGACGC CCTTGATCCT GGCAGCGAGA 
CTAGCTATTG AAGGGATGGT TCAGGATTTG ATTAGTGCTG AAGCTGATAT TAATGCGGCG 
GATAATTCGG GGAAGACGGC ATTGCATTGG GCCGCTGCTG TTAATAATGT GGAGGCGGTT 
AATGTGTTGC TGGCCCATGG GGCCAATCGA GATGCACAAG ATGATAAGGA TGAAACGCCT 
CTGTTTCTTG CGGCAAGAGA AGGCTCATAT CAGGCGTGTA AAGCACTACT TGAAGCTTAC 
GCTAATCGGG AAATTACCGA CCACATGGAT AGATTGCCAC GAGATGTCGC CAGTGAACGT 
TTACATCATG ATATAGTGCG ACTTTTGGAT GAACATGTCC CAAGAAGTCC ACAAATGGTC 
AATGTTATAC CCAATAGTTC GATGATGACA TCACCTAGCG GCCAATTACA ATTACAACAA 
CCAACAGTAA TCGTCCCAAA ACGTCAAAAA TCAAAAAGGC CGAGTAAAAA CTCAAATAAT 
AACAATAACG CATCGTTGGA CCCGACAAGT CCCGAAATGG AAACGATCAA CACCGGAAGT 
ATCCGACGTA AACCGAGCGT GAAAAAATCA AAGAAACCAA CCCCACAACA AGAAATCCCC 
CAAAGTGTTG ATTCAATAAC TTCAACCTTA AGTCCAGTTG AATCCCCAAT GACGACAAAT 
CTACCGAGTC CTTACGACAC TGCATCGATT TACTCAAATA TGGGCCTAAC ACCACAAGGA 
CTTGAGTCAT TGATTAATAA ACAACCACCA AGCTATGAAG ATGTGGTTAA GAATAATTTA 
CACGGTCTAG TTGGCTTGGA AAATTACTCG TTCAATTTGG GTAATTTCCA CGATACGATG 
ATGATGCAGC AGCGCCAATT ACAACAAGCC AACACCTTAA GCCCGCCCTA TTCGAATCAA 
TCCCCGCCCC ATTCCGTGCA ATCGAATATG TCGTTATCGC CGCAAGGTTA TAACGGTTCA 
CCATCCCCGG CCAAGACACG CCCCTCGCTC CCGACAAGCC CCACCCATAT AGCGGCAATG 
CGTGCCGCCA CCCAACAGAA GCACGGAGGC GTGCCACAAG CACAAAATTT ACAAATGGGT 
TTTGATTTTA GTCAAACTGG ATTGGAGTTG CCAATGGCGA CATATCCAGG ATTGGTAAGT 
GGAAATTTAC AATTTCAACA ACAAAACTAT TATCTGACTC CGCCCTCACA ACACTCCGAT 
CTCTTACCGG ATAATTTTCC AACACCGAGT CCGGAAAGTC CGGGTCACTG GTCGTCGAGT 
TCACCCCGGA GTTGTAATTC GGATTGGTCC GAAAATGTCG CCAGTCCGAA TAATACTTAT 
GGGGGTGGAC ATCAGACTAA CAAAGGCTCG GATGCGATCT ATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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N cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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