LOC660900 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660900
Entrez Gene ID	660900
Full Name	CAD protein
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_967097.3	XP_972190.1	CAD protein

Pathways from BioSystems
Gene Ontology

The following LOC660900 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660900 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg12778	XM_967097.3 Latest version!	Tribolium castaneum CAD protein (LOC660900), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660900 ( XM_967097.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_972190.1 Tribolium castaneum CAD protein (LOC660900), mRNA.

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CloneID	OTg12778
Clone ID Related Accession (Same CDS sequence)	XM_967097.3
Accession Version	XM_967097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	CAD protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_967097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAA GTGACGAAAG ACACACTTGT TTTCTTCTCC TCGAGGATGG GACTGTACTA 
CCGGGTGAAT CGTTTGGGGC TCGAAACCAA ATCGATGGGG AAGTTGTTTT CCAGACTGGG 
ATGGTCGGTT ACCCCGAATC CCTAACCGAC CCCAGCTACC ATGCCCAGAT CCTGGTCCTC 
ACATACCCCC TCGTGGGTAA TTACGGTGTC CCCAAAGCGG TCGAAGACCC GCACGGGTTG 
CCCTATTTTT TCGAATCGCA CCGGATTTGG GCCGCTGGTC TCGTCGTGGG TGAACACTGC 
GACAATCCGA GCCATTGGCA CAACTTCAGG ACACTTTCCG ACTGGATGAA GTCGGAAAAT 
GTCCCCGGGA TCCAAGGGAT TGACACTAGG GCCTTGACCA AGAAAATTCG CGAAAAGGGG 
ACGATTTTGG GCCGGATTGT TTATAGTTTG CCCATTGTGA CCGAAAATTT GAAAATTGTT 
GACCCCAATT TGAGGAATTT GGTCGAGGAA GTGTCAGTTA AGAAAATTGT GACGTATAAT 
TCCGGGGGGA GTCCCAGGAT TTGTGCCGTT GATTGCGGCT TGAAATACAA TCAAATTCGC 
TGTTTGGTTA ATAGAGGGGC TCGGGTTGAT TTGGTACCAT GGGACCACAA ACTCAATATC 
AACGACTATG ATGGTCTTTT TCTCAGCAAT GGTCCTGGGG ACCCAACCAT GTGCCAGGTC 
ACTATCAATA ACATCAAAGA CGTGATTAAG GGACCAACAA TTAAACCGAT TTTTGGTATT 
TGTCTCGGTC ACCAACTCCT TTCAGTGGCC ATTGGGTGCA AAAGCTACAA AATGAAGTAT 
GGTAATCGTG GTCACAACCA ACCATGCACC CATCATGGTA CCAACCGGTG TTACATGACC 
TCGCAAAACC ACGGTTTTGC CATCAATACG GAGACACTTC CCAACGACTG GGAAATCCTC 
TTCACGAATG CTAACGACAA GACCAACGAA GGAATAATTC ACTCAAAACT GCCGTTTTTC 
AGCGTACAGT TCCACCCCGA ACACACAGCT GGGCCCCAGG ACCTTGAATG CCTCTTCGAC 
GTGTTCCTGG ACCTGGTTAA GAACCCCAAC CAGACAGCTA AGACTCTCCT TACCCAAAAA 
CTAACCTATT CGGGCACCCC TCAGTTGCCC GAACGCCCCA AAAAAGTGCT AATAATAGGT 
TCCGGGGGCC TTTCCATCGG CCAAGCGGGC GAGTTTGACT ATTCCGGCTC CCAGGCCATT 
AAAGCCCTTC GTGAGGAAAA CATCCAAACT GTTTTGATCA ACCCCAACAT CGCCACAGTC 
CAAACCTCCA AAGGTTTAGC CGACAAAGTC TACTTCTTGC CTCTAGTACC CGACTACGTG 
GAGCAAGTCA TTCGAGTCGA GCGCCCCGGA GGCGTGCTGT TAACCTTCGG GGGGCAAACG 
GGCCTCAACT GTGGGGTCGA GCTCCAAAAA GCCGGCATCT TCGACAAGTA CAACATCCAA 
ATTCTGGGGA CCCCCATCCA GGCTATAATC GATACGGAAG ACCGGAAGAT CTTCACCGAA 
CGTATTGCCG CCATTGGGGA GAAGGTGGCC CCCAGCATGG CGGCGCACTC CGTGGAGGAG 
GCTCTGGAGG CGGCCGAGCA ACTGGGGTAC CCGGTCATGG CCCGCGCTGC CTTTTCCCTA 
GGCGGACTTG GGTCGGGCTT CGCCAATAGT AAGGACGAAC TTAAGACTTT GGCCGTCCAG 
GCTTTGGCCC ACTCGAGTCA ATTGATCATC GACAAGTCGC TCAAAGGCTG GAAGGAGGTG 
GAGTACGAGG TGGTGCGGGA CGCCTTCGAC AACTGCATCA CGGTGTGCAA CATGGAGAAC 
GTCGACCCTT TGGGGATACA CACCGGGGAG TCGATTGTGG TCGCCCCCAG CCAGACCCTT 
TCGAACCGGG AGTACAACAT GCTGCGCACC ACCGCCATTA AGGTGATTCG GCACTTCGGG 
GTCATCGGCG AGTGCAACAT CCAGTACGCC GTCAATCCCA ACTCCGAGGA GTTCTACATC 
ATCGAGGTTA ATGCTAGGTT ATCGCGAAGC TCGGCCCTGG CCAGTAAAGC CACAGGGTAC 
CCCCTAGCCT ACATCGCGGC CAAGCTGGCC TTGGGGGTAC CCCTCCCTGA GATCAATAAT 
TCCGTGACTG GGGTCACAAC TGCGTGTTTT GAGCCAAGTC TTGACTACTG CGTCGTCAAA 
ATCCCCCGCT GGGACTTGCA CAAGTTCTCC CGCGTCAGTA CCAAAATCGG AAGTTCGATG 
AAAAGTGTGG GGGAGGTGAT GGCAATCGGG CGCAAGTTCG AAGAAGCGTT CCAGAAGGCC 
TTGCGCATGG TTGACGAGAA CGTGATGGGG TTTGACCCCT ATCTCAAGGA CGTTAACGAC 
GAGGAGTTGA AGGAACCCAC CGACAAGAGG ATGTTTGTGG TGGCGGCGGC CCTCAAGAAA 
GGCTATTCGG TCGATAGGCT GTACGACTTG ACCAAAATCG ACCGCTGGTT TTTGGAGAAG 
ATGAAGAAGA TCGTCGACTG GACCACGTTC CTCGAAACCA TAGACCAGCT GCACTTGTCG 
CATGATATTT TACTGAAGGC CAAACAAATC GGGTTCTCCG ATAAACAGAT TGCGACAGCA 
GTCAAGAGTA CCGAACTGGC CATCAGGAAG CAACGCGAGG AGTTCGGGAT CAAGCCATAC 
GTCAAGCAGA TAGACACAGT GGCGGCGGAA TGGCCCGCCA CCACCAACTA CCTCTACCTA 
ACCTACAACG CCTTTGAGCA CGACTTGACC TTCGAGGAAA AACACACCAT GGTCATAGGA 
TCGGGGGTTT ACCGCATCGG GAGCTCCGTG GAGTTCGACT GGTGCGCAGT CGGGTGCCTC 
CGGGAGTTGC GCCGCTTGGG CCGCAAGACA ATCATGATCA ACTACAACCC CGAAACCGTC 
AGCACCGACT ACGACATGTC GGACCGCCTC TACTTTGAAG AAATCTCCTT TGAAGTGGTC 
ATGGACATCT ACAACATCGA GGACCCTGTC GGGATCATAC TGTCAATGGG CGGCCAACTC 
CCCAACAACA TCGCCATCGA CCTCCACCGG CAGCAAGCCC GCATCTTGGG CACGTCCCCC 
GACTCCATCG ACGGCGCCGA GAACCGCTTC AAATTCTCGC GGATGTTGGA CCGGATCGGG 
ATCATGCAGC CGCGTTGGAA GGAACTCACT AACCTCAAAT CGGCGATCGA GTTCTGCGAA 
GAAGTGGGGT ACCCGTGCCT GGTGCGCCCC TCCTACGTGC TCAGCGGCGC CGCCATGAAC 
GTTGCCCACT CCAACCAGGA CTTGGAGACG TACCTGAACG CCGCTAGCGA CGTCTCCAAG 
GAGCACCCAG TCGTCATCTC GAAGTTCATC CTCGAGTCGA AGGAGATCGA CGTCGATGCG 
GTGGCCTGCG ACGGCGTTAT CCTCTGCATG GCGGTGTCGG AGCACGTGGA GAACGCCGGG 
GTGCACTCCG GGGACGCCAC ACTCGTTACC CCGCCGCAGG ACATCAACGA CGAGACTCTG 
GGCAAGATCA CGCAGATCTG CAAGGCCATT GCGGCCTCGC TGGAGGTGAC TGGGCCCTTC 
AACATGCAAC TGATCGCCAA GGACAATGAT CTGAAAGTGA TCGAGTGTAA CGTGCGCGTT 
TCGCGTTCCT TCCCCTTTGT TTCCAAAACC CTAGACCATG ATTTTGTGGC GATGGCCACT 
CGGGTGATGG TTGGGGAGCT GGTCCAGCCC GTGGATGTGC TCTCGGGGTG CGGCAAAGTG 
GGGGTTAAGG TGCCCCAGTT TTCGTTTTCG CGGCTCGCGG GCGCCGATTT TATGCTGGGG 
GTGGAGATGG CGTCCACTGG GGAGGTGGCC TGTTTTGGGG ATAATCGGTA CGAGGCGTAC 
TTGAAGGCCA TGATGAGTAC TGGGTTTAGC GTCCCCGAAA AATCGATTCT GCTGTCAATT 
GGGAGTTTCA AGCATAAAAT GGAGCTGCTG CCCAGCATGA GAGTGTTGCA AAAGATGGGG 
TACAAGTTGT ATGCAAGTTT GGGCACCGCT GACTTTTACA ACGAGCATGG AGTTAAGGTC 
GAGTCGGTAC AATGGACTTT CGAGAACATT GATGCCATAA CGAGCCAAGG GGAGCTCAAG 
CACTTGGCGG AGTTTTTGTC AAAGAAACAG TTTGACTTGG TTATCAACCT CCCCATGCGC 
AATGGCGGCG CTCGGAGAGT GTCCTCCTTC ATGACCCACG GGTACAGGAC CCGGCGCCTG 
GCCATCGACT ACTCCATCCC GCTCGTGACC GACGTCAAAT GCACCAAACT CCTGGTGGAG 
GCTCTGAGAC GCATTGGGAC CGCGCCCCCC ATGAAAACCC ACACTGACTG CATGTCTTCG 
CGCCGTTTGG TCAAACTACC GGGCCTTATT GACACCCACG TCCACGTGCG TGAACCCGGG 
GCCACCCACA AGGAGGACTA CGCCTCCTGC ACGGCCGCAG CCCTCGCTGG CGGCGTCACC 
ATGATTTGCG CGATGCCCAA CACTAACCCG GCCGTCACCA ACCAGGAAAC CCTAACCCTT 
GTGGAGAACC TGGCGCGGAA AAACGCCCGG TGTGACTATG CCATCTACAT GGGGGCCTCC 
TCAGACAACT ACTCCGCGAT CCCGGAGCTC GCCCCCCAGG TCGCAGGCCT CAAAATGTAC 
CTCAATGAGA CCTTCACCAC GTTGAGACTT AACGATCTCA ACACCTGGAC CAAGCACTTG 
GCTAATTGGC CGAAAAAAGC CCCGATTTGT GTGCATGCCG AGGGGCAAAC CACGGCCGCT 
GTTATCCTGA TGGCGAGTTT GCAAAACCGG CCAATCCACA TCTGTCACGT GGCCCGGAAA 
GAGGAAATCT TGATCATCAA AGCGGCCAAG GAAAAAGGCT TGAACATAAC CTGTGAGGTT 
TGCCCCCACC ACCTTTTTCT CTCGACTGAA GATGTCAAAA CCTTGGGGGT GTGTAAAAGT 
CAGGTTCGGC CTGTTCTAAT GTCACCAGAG GACCAACAAG CCCTTTGGGA TAACTTACAC 
GTGATTGACT GCTTTGCCAC CGACCATGCC CCCCACACTT TGGAGGAGAA GACCAGTTCG 
AGCCCTCCCC CAGGCTTCCC CGGGCTGGAG ACAATCCTCC CGTTGCTCCT GAACGCCGTT 
AACGAGAACA AACTGACTAT TGACGACATC GTCACCAAGT TCTACCGCAA CCCCAAGAAG 
ATTTTCAATT TGCCGGACCA ACCAAACACT TACGTTGAAA TCGACCTGGA CGAGGAGTGG 
ACGATTCCAG AGGCTACCAC TTACAGTAAG GCTAAGTGGA CCCCGTTCGC TGGCCGCAAG 
GTCAAGGGAG CCGTCCACCG CGTGGTGCTC CGTGGAGAGG TGGCTTTCGT CGACGGCCAG 
ATCCTGGTGC AGCCCGGGTT TGGGCAAAAC GTGCGCACGT GGGACAAAAA GACCTTCGAC 
GAGGTCATAG CCCCTCCCAC CGTCACCACT GGGGAGTACA ACGACGGGCA GACCAACGAG 
ATTTTCAAGA AGTTGCTGTC AGGGGGTGGC AAAGTGCACT TCTCTGCCAC CAACGAGCTG 
CACGTGGACA GCCGGCTGGG GGTGACCTCG CCCACGCCCG TGGGGCCCCG GATCCGCACT 
GGTAGTTTCT CCCAAATGGA GCAGATGCAT CACAGTCTTG CCAACAAGCA TATTCTTTCG 
GTGGATATGT TTACGAAAGA CCAGCTCAAT GATATTTTCA ACTTGGCGCA AACTTTTCGC 
GTTAATGTCA GTAAGGAGCG GGCTGTTGAT CATATCTTGA AGGGGAAAGT CATGGCGTCG 
ATTTTCTACG AGGTTAGCAC TCGGACCAGT TGCAGTTTCG CGGCTGCCAT GCAGAGGTTG 
GGCGGTGGGG TGATTTATAT GGACGAGACG AGTTCGTCGG TGAAGAAAGG GGAGACTTTG 
GAGGACTCGG TTGCTGTGAT GGCGGGGTAT GCCGATGTGG TGGTGTTGAG GCATCCGACG 
CCTGGCTCGG TTATGCGGGC GTCCCACCAC TGCCGCAAGC CCCTCATCAA CGCCGGCGAC 
GGAGTCGGCG AGCACCCCAG CCAAGCCCTC CTCGATATTT TCACAATTCG AGAGGAGATC 
GGCACTGTGA ACGGCCTCAC CATTACCCTA GTTGGTGATT TGAAAAACGG GCGCACTGTT 
CATTCCCTTG CACGTTTGCT GACACTGTAC AACGTGCAGC TGCGTTACGT AAGCCCCCCG 
AGCTTGAAAA TGCCGAGTCA TATCACCAGC TTCGTGGCCT CCAAAGGCAT ACCCCAGAAC 
GAGCACAGTA CCCTTGAGGA GGTACTGCCG GACACGGACG TACTGTACAT GACTCGCATC 
CAGAGAGAAC GGTTTAGCTC ACAAGAGGAG TACGACAAGG CCTGTGGCCA CTTTATCGTA 
ACTCCGCAAC TGATGACGAG GGCGAAGAGG AAAATGGTGG TGCTTCACCC CCTGCCCAGG 
GTGTTCGAAA TCAGCCCGGA GTTTGACACG GACCCCCGGG CGGCCTATTT CAGGCAGGCG 
GAGTGCGGGA TGTATGTCAG AATGGCGCTC CTAGCCATGG TTTTAGGGAA GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_972190.1
CDS	265..6861
Translation	MTKSDERHTCFLLLEDGTVLPGESFGARNQIDGEVVFQTGMVGYPESLTDPSYHAQILVLTYPLVGNYGVPKAVEDPHGLPYFFESHRIWAAGLVVGEHCDNPSHWHNFRTLSDWMKSENVPGIQGIDTRALTKKIREKGTILGRIVYSLPIVTENLKIVDPNLRNLVEEVSVKKIVTYNSGGSPRICAVDCGLKYNQIRCLVNRGARVDLVPWDHKLNINDYDGLFLSNGPGDPTMCQVTINNIKDVIKGPTIKPIFGICLGHQLLSVAIGCKSYKMKYGNRGHNQPCTHHGTNRCYMTSQNHGFAINTETLPNDWEILFTNANDKTNEGIIHSKLPFFSVQFHPEHTAGPQDLECLFDVFLDLVKNPNQTAKTLLTQKLTYSGTPQLPERPKKVLIIGSGGLSIGQAGEFDYSGSQAIKALREENIQTVLINPNIATVQTSKGLADKVYFLPLVPDYVEQVIRVERPGGVLLTFGGQTGLNCGVELQKAGIFDKYNIQILGTPIQAIIDTEDRKIFTERIAAIGEKVAPSMAAHSVEEALEAAEQLGYPVMARAAFSLGGLGSGFANSKDELKTLAVQALAHSSQLIIDKSLKGWKEVEYEVVRDAFDNCITVCNMENVDPLGIHTGESIVVAPSQTLSNREYNMLRTTAIKVIRHFGVIGECNIQYAVNPNSEEFYIIEVNARLSRSSALASKATGYPLAYIAAKLALGVPLPEINNSVTGVTTACFEPSLDYCVVKIPRWDLHKFSRVSTKIGSSMKSVGEVMAIGRKFEEAFQKALRMVDENVMGFDPYLKDVNDEELKEPTDKRMFVVAAALKKGYSVDRLYDLTKIDRWFLEKMKKIVDWTTFLETIDQLHLSHDILLKAKQIGFSDKQIATAVKSTELAIRKQREEFGIKPYVKQIDTVAAEWPATTNYLYLTYNAFEHDLTFEEKHTMVIGSGVYRIGSSVEFDWCAVGCLRELRRLGRKTIMINYNPETVSTDYDMSDRLYFEEISFEVVMDIYNIEDPVGIILSMGGQLPNNIAIDLHRQQARILGTSPDSIDGAENRFKFSRMLDRIGIMQPRWKELTNLKSAIEFCEEVGYPCLVRPSYVLSGAAMNVAHSNQDLETYLNAASDVSKEHPVVISKFILESKEIDVDAVACDGVILCMAVSEHVENAGVHSGDATLVTPPQDINDETLGKITQICKAIAASLEVTGPFNMQLIAKDNDLKVIECNVRVSRSFPFVSKTLDHDFVAMATRVMVGELVQPVDVLSGCGKVGVKVPQFSFSRLAGADFMLGVEMASTGEVACFGDNRYEAYLKAMMSTGFSVPEKSILLSIGSFKHKMELLPSMRVLQKMGYKLYASLGTADFYNEHGVKVESVQWTFENIDAITSQGELKHLAEFLSKKQFDLVINLPMRNGGARRVSSFMTHGYRTRRLAIDYSIPLVTDVKCTKLLVEALRRIGTAPPMKTHTDCMSSRRLVKLPGLIDTHVHVREPGATHKEDYASCTAAALAGGVTMICAMPNTNPAVTNQETLTLVENLARKNARCDYAIYMGASSDNYSAIPELAPQVAGLKMYLNETFTTLRLNDLNTWTKHLANWPKKAPICVHAEGQTTAAVILMASLQNRPIHICHVARKEEILIIKAAKEKGLNITCEVCPHHLFLSTEDVKTLGVCKSQVRPVLMSPEDQQALWDNLHVIDCFATDHAPHTLEEKTSSSPPPGFPGLETILPLLLNAVNENKLTIDDIVTKFYRNPKKIFNLPDQPNTYVEIDLDEEWTIPEATTYSKAKWTPFAGRKVKGAVHRVVLRGEVAFVDGQILVQPGFGQNVRTWDKKTFDEVIAPPTVTTGEYNDGQTNEIFKKLLSGGGKVHFSATNELHVDSRLGVTSPTPVGPRIRTGSFSQMEQMHHSLANKHILSVDMFTKDQLNDIFNLAQTFRVNVSKERAVDHILKGKVMASIFYEVSTRTSCSFAAAMQRLGGGVIYMDETSSSVKKGETLEDSVAVMAGYADVVVLRHPTPGSVMRASHHCRKPLINAGDGVGEHPSQALLDIFTIREEIGTVNGLTITLVGDLKNGRTVHSLARLLTLYNVQLRYVSPPSLKMPSHITSFVASKGIPQNEHSTLEEVLPDTDVLYMTRIQRERFSSQEEYDKACGHFIVTPQLMTRAKRKMVVLHPLPRVFEISPEFDTDPRAAYFRQAECGMYVRMALLAMVLGKC

Target ORF information:

RefSeq Version	XM_967097.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum CAD protein (LOC660900), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_967097.3

ORF Insert Sequence:

ATGACTAAAA GTGACGAAAG ACACACTTGT TTTCTTCTCC TCGAGGATGG GACTGTACTA 
CCGGGTGAAT CGTTTGGGGC TCGAAACCAA ATCGATGGGG AAGTTGTTTT CCAGACTGGG 
ATGGTCGGTT ACCCCGAATC CCTAACCGAC CCCAGCTACC ATGCCCAGAT CCTGGTCCTC 
ACATACCCCC TCGTGGGTAA TTACGGTGTC CCCAAAGCGG TCGAAGACCC GCACGGGTTG 
CCCTATTTTT TCGAATCGCA CCGGATTTGG GCCGCTGGTC TCGTCGTGGG TGAACACTGC 
GACAATCCGA GCCATTGGCA CAACTTCAGG ACACTTTCCG ACTGGATGAA GTCGGAAAAT 
GTCCCCGGGA TCCAAGGGAT TGACACTAGG GCCTTGACCA AGAAAATTCG CGAAAAGGGG 
ACGATTTTGG GCCGGATTGT TTATAGTTTG CCCATTGTGA CCGAAAATTT GAAAATTGTT 
GACCCCAATT TGAGGAATTT GGTCGAGGAA GTGTCAGTTA AGAAAATTGT GACGTATAAT 
TCCGGGGGGA GTCCCAGGAT TTGTGCCGTT GATTGCGGCT TGAAATACAA TCAAATTCGC 
TGTTTGGTTA ATAGAGGGGC TCGGGTTGAT TTGGTACCAT GGGACCACAA ACTCAATATC 
AACGACTATG ATGGTCTTTT TCTCAGCAAT GGTCCTGGGG ACCCAACCAT GTGCCAGGTC 
ACTATCAATA ACATCAAAGA CGTGATTAAG GGACCAACAA TTAAACCGAT TTTTGGTATT 
TGTCTCGGTC ACCAACTCCT TTCAGTGGCC ATTGGGTGCA AAAGCTACAA AATGAAGTAT 
GGTAATCGTG GTCACAACCA ACCATGCACC CATCATGGTA CCAACCGGTG TTACATGACC 
TCGCAAAACC ACGGTTTTGC CATCAATACG GAGACACTTC CCAACGACTG GGAAATCCTC 
TTCACGAATG CTAACGACAA GACCAACGAA GGAATAATTC ACTCAAAACT GCCGTTTTTC 
AGCGTACAGT TCCACCCCGA ACACACAGCT GGGCCCCAGG ACCTTGAATG CCTCTTCGAC 
GTGTTCCTGG ACCTGGTTAA GAACCCCAAC CAGACAGCTA AGACTCTCCT TACCCAAAAA 
CTAACCTATT CGGGCACCCC TCAGTTGCCC GAACGCCCCA AAAAAGTGCT AATAATAGGT 
TCCGGGGGCC TTTCCATCGG CCAAGCGGGC GAGTTTGACT ATTCCGGCTC CCAGGCCATT 
AAAGCCCTTC GTGAGGAAAA CATCCAAACT GTTTTGATCA ACCCCAACAT CGCCACAGTC 
CAAACCTCCA AAGGTTTAGC CGACAAAGTC TACTTCTTGC CTCTAGTACC CGACTACGTG 
GAGCAAGTCA TTCGAGTCGA GCGCCCCGGA GGCGTGCTGT TAACCTTCGG GGGGCAAACG 
GGCCTCAACT GTGGGGTCGA GCTCCAAAAA GCCGGCATCT TCGACAAGTA CAACATCCAA 
ATTCTGGGGA CCCCCATCCA GGCTATAATC GATACGGAAG ACCGGAAGAT CTTCACCGAA 
CGTATTGCCG CCATTGGGGA GAAGGTGGCC CCCAGCATGG CGGCGCACTC CGTGGAGGAG 
GCTCTGGAGG CGGCCGAGCA ACTGGGGTAC CCGGTCATGG CCCGCGCTGC CTTTTCCCTA 
GGCGGACTTG GGTCGGGCTT CGCCAATAGT AAGGACGAAC TTAAGACTTT GGCCGTCCAG 
GCTTTGGCCC ACTCGAGTCA ATTGATCATC GACAAGTCGC TCAAAGGCTG GAAGGAGGTG 
GAGTACGAGG TGGTGCGGGA CGCCTTCGAC AACTGCATCA CGGTGTGCAA CATGGAGAAC 
GTCGACCCTT TGGGGATACA CACCGGGGAG TCGATTGTGG TCGCCCCCAG CCAGACCCTT 
TCGAACCGGG AGTACAACAT GCTGCGCACC ACCGCCATTA AGGTGATTCG GCACTTCGGG 
GTCATCGGCG AGTGCAACAT CCAGTACGCC GTCAATCCCA ACTCCGAGGA GTTCTACATC 
ATCGAGGTTA ATGCTAGGTT ATCGCGAAGC TCGGCCCTGG CCAGTAAAGC CACAGGGTAC 
CCCCTAGCCT ACATCGCGGC CAAGCTGGCC TTGGGGGTAC CCCTCCCTGA GATCAATAAT 
TCCGTGACTG GGGTCACAAC TGCGTGTTTT GAGCCAAGTC TTGACTACTG CGTCGTCAAA 
ATCCCCCGCT GGGACTTGCA CAAGTTCTCC CGCGTCAGTA CCAAAATCGG AAGTTCGATG 
AAAAGTGTGG GGGAGGTGAT GGCAATCGGG CGCAAGTTCG AAGAAGCGTT CCAGAAGGCC 
TTGCGCATGG TTGACGAGAA CGTGATGGGG TTTGACCCCT ATCTCAAGGA CGTTAACGAC 
GAGGAGTTGA AGGAACCCAC CGACAAGAGG ATGTTTGTGG TGGCGGCGGC CCTCAAGAAA 
GGCTATTCGG TCGATAGGCT GTACGACTTG ACCAAAATCG ACCGCTGGTT TTTGGAGAAG 
ATGAAGAAGA TCGTCGACTG GACCACGTTC CTCGAAACCA TAGACCAGCT GCACTTGTCG 
CATGATATTT TACTGAAGGC CAAACAAATC GGGTTCTCCG ATAAACAGAT TGCGACAGCA 
GTCAAGAGTA CCGAACTGGC CATCAGGAAG CAACGCGAGG AGTTCGGGAT CAAGCCATAC 
GTCAAGCAGA TAGACACAGT GGCGGCGGAA TGGCCCGCCA CCACCAACTA CCTCTACCTA 
ACCTACAACG CCTTTGAGCA CGACTTGACC TTCGAGGAAA AACACACCAT GGTCATAGGA 
TCGGGGGTTT ACCGCATCGG GAGCTCCGTG GAGTTCGACT GGTGCGCAGT CGGGTGCCTC 
CGGGAGTTGC GCCGCTTGGG CCGCAAGACA ATCATGATCA ACTACAACCC CGAAACCGTC 
AGCACCGACT ACGACATGTC GGACCGCCTC TACTTTGAAG AAATCTCCTT TGAAGTGGTC 
ATGGACATCT ACAACATCGA GGACCCTGTC GGGATCATAC TGTCAATGGG CGGCCAACTC 
CCCAACAACA TCGCCATCGA CCTCCACCGG CAGCAAGCCC GCATCTTGGG CACGTCCCCC 
GACTCCATCG ACGGCGCCGA GAACCGCTTC AAATTCTCGC GGATGTTGGA CCGGATCGGG 
ATCATGCAGC CGCGTTGGAA GGAACTCACT AACCTCAAAT CGGCGATCGA GTTCTGCGAA 
GAAGTGGGGT ACCCGTGCCT GGTGCGCCCC TCCTACGTGC TCAGCGGCGC CGCCATGAAC 
GTTGCCCACT CCAACCAGGA CTTGGAGACG TACCTGAACG CCGCTAGCGA CGTCTCCAAG 
GAGCACCCAG TCGTCATCTC GAAGTTCATC CTCGAGTCGA AGGAGATCGA CGTCGATGCG 
GTGGCCTGCG ACGGCGTTAT CCTCTGCATG GCGGTGTCGG AGCACGTGGA GAACGCCGGG 
GTGCACTCCG GGGACGCCAC ACTCGTTACC CCGCCGCAGG ACATCAACGA CGAGACTCTG 
GGCAAGATCA CGCAGATCTG CAAGGCCATT GCGGCCTCGC TGGAGGTGAC TGGGCCCTTC 
AACATGCAAC TGATCGCCAA GGACAATGAT CTGAAAGTGA TCGAGTGTAA CGTGCGCGTT 
TCGCGTTCCT TCCCCTTTGT TTCCAAAACC CTAGACCATG ATTTTGTGGC GATGGCCACT 
CGGGTGATGG TTGGGGAGCT GGTCCAGCCC GTGGATGTGC TCTCGGGGTG CGGCAAAGTG 
GGGGTTAAGG TGCCCCAGTT TTCGTTTTCG CGGCTCGCGG GCGCCGATTT TATGCTGGGG 
GTGGAGATGG CGTCCACTGG GGAGGTGGCC TGTTTTGGGG ATAATCGGTA CGAGGCGTAC 
TTGAAGGCCA TGATGAGTAC TGGGTTTAGC GTCCCCGAAA AATCGATTCT GCTGTCAATT 
GGGAGTTTCA AGCATAAAAT GGAGCTGCTG CCCAGCATGA GAGTGTTGCA AAAGATGGGG 
TACAAGTTGT ATGCAAGTTT GGGCACCGCT GACTTTTACA ACGAGCATGG AGTTAAGGTC 
GAGTCGGTAC AATGGACTTT CGAGAACATT GATGCCATAA CGAGCCAAGG GGAGCTCAAG 
CACTTGGCGG AGTTTTTGTC AAAGAAACAG TTTGACTTGG TTATCAACCT CCCCATGCGC 
AATGGCGGCG CTCGGAGAGT GTCCTCCTTC ATGACCCACG GGTACAGGAC CCGGCGCCTG 
GCCATCGACT ACTCCATCCC GCTCGTGACC GACGTCAAAT GCACCAAACT CCTGGTGGAG 
GCTCTGAGAC GCATTGGGAC CGCGCCCCCC ATGAAAACCC ACACTGACTG CATGTCTTCG 
CGCCGTTTGG TCAAACTACC GGGCCTTATT GACACCCACG TCCACGTGCG TGAACCCGGG 
GCCACCCACA AGGAGGACTA CGCCTCCTGC ACGGCCGCAG CCCTCGCTGG CGGCGTCACC 
ATGATTTGCG CGATGCCCAA CACTAACCCG GCCGTCACCA ACCAGGAAAC CCTAACCCTT 
GTGGAGAACC TGGCGCGGAA AAACGCCCGG TGTGACTATG CCATCTACAT GGGGGCCTCC 
TCAGACAACT ACTCCGCGAT CCCGGAGCTC GCCCCCCAGG TCGCAGGCCT CAAAATGTAC 
CTCAATGAGA CCTTCACCAC GTTGAGACTT AACGATCTCA ACACCTGGAC CAAGCACTTG 
GCTAATTGGC CGAAAAAAGC CCCGATTTGT GTGCATGCCG AGGGGCAAAC CACGGCCGCT 
GTTATCCTGA TGGCGAGTTT GCAAAACCGG CCAATCCACA TCTGTCACGT GGCCCGGAAA 
GAGGAAATCT TGATCATCAA AGCGGCCAAG GAAAAAGGCT TGAACATAAC CTGTGAGGTT 
TGCCCCCACC ACCTTTTTCT CTCGACTGAA GATGTCAAAA CCTTGGGGGT GTGTAAAAGT 
CAGGTTCGGC CTGTTCTAAT GTCACCAGAG GACCAACAAG CCCTTTGGGA TAACTTACAC 
GTGATTGACT GCTTTGCCAC CGACCATGCC CCCCACACTT TGGAGGAGAA GACCAGTTCG 
AGCCCTCCCC CAGGCTTCCC CGGGCTGGAG ACAATCCTCC CGTTGCTCCT GAACGCCGTT 
AACGAGAACA AACTGACTAT TGACGACATC GTCACCAAGT TCTACCGCAA CCCCAAGAAG 
ATTTTCAATT TGCCGGACCA ACCAAACACT TACGTTGAAA TCGACCTGGA CGAGGAGTGG 
ACGATTCCAG AGGCTACCAC TTACAGTAAG GCTAAGTGGA CCCCGTTCGC TGGCCGCAAG 
GTCAAGGGAG CCGTCCACCG CGTGGTGCTC CGTGGAGAGG TGGCTTTCGT CGACGGCCAG 
ATCCTGGTGC AGCCCGGGTT TGGGCAAAAC GTGCGCACGT GGGACAAAAA GACCTTCGAC 
GAGGTCATAG CCCCTCCCAC CGTCACCACT GGGGAGTACA ACGACGGGCA GACCAACGAG 
ATTTTCAAGA AGTTGCTGTC AGGGGGTGGC AAAGTGCACT TCTCTGCCAC CAACGAGCTG 
CACGTGGACA GCCGGCTGGG GGTGACCTCG CCCACGCCCG TGGGGCCCCG GATCCGCACT 
GGTAGTTTCT CCCAAATGGA GCAGATGCAT CACAGTCTTG CCAACAAGCA TATTCTTTCG 
GTGGATATGT TTACGAAAGA CCAGCTCAAT GATATTTTCA ACTTGGCGCA AACTTTTCGC 
GTTAATGTCA GTAAGGAGCG GGCTGTTGAT CATATCTTGA AGGGGAAAGT CATGGCGTCG 
ATTTTCTACG AGGTTAGCAC TCGGACCAGT TGCAGTTTCG CGGCTGCCAT GCAGAGGTTG 
GGCGGTGGGG TGATTTATAT GGACGAGACG AGTTCGTCGG TGAAGAAAGG GGAGACTTTG 
GAGGACTCGG TTGCTGTGAT GGCGGGGTAT GCCGATGTGG TGGTGTTGAG GCATCCGACG 
CCTGGCTCGG TTATGCGGGC GTCCCACCAC TGCCGCAAGC CCCTCATCAA CGCCGGCGAC 
GGAGTCGGCG AGCACCCCAG CCAAGCCCTC CTCGATATTT TCACAATTCG AGAGGAGATC 
GGCACTGTGA ACGGCCTCAC CATTACCCTA GTTGGTGATT TGAAAAACGG GCGCACTGTT 
CATTCCCTTG CACGTTTGCT GACACTGTAC AACGTGCAGC TGCGTTACGT AAGCCCCCCG 
AGCTTGAAAA TGCCGAGTCA TATCACCAGC TTCGTGGCCT CCAAAGGCAT ACCCCAGAAC 
GAGCACAGTA CCCTTGAGGA GGTACTGCCG GACACGGACG TACTGTACAT GACTCGCATC 
CAGAGAGAAC GGTTTAGCTC ACAAGAGGAG TACGACAAGG CCTGTGGCCA CTTTATCGTA 
ACTCCGCAAC TGATGACGAG GGCGAAGAGG AAAATGGTGG TGCTTCACCC CCTGCCCAGG 
GTGTTCGAAA TCAGCCCGGA GTTTGACACG GACCCCCGGG CGGCCTATTT CAGGCAGGCG 
GAGTGCGGGA TGTATGTCAG AATGGCGCTC CTAGCCATGG TTTTAGGGAA GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC660900 cDNA ORF clone, Tribolium castaneum(red flour beetle)

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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