ORF » Species Summary » Tribolium castaneum » LOC660690 cDNA ORF clone

LOC660690 cDNA ORF clone, Tribolium castaneum(red flour beetle)

The following LOC660690 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660690 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OTg00493 XM_008201341.2
Latest version!		 Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OTg00494 XM_008201346.2
Latest version!		 Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC660690 ( XM_008201341.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199563.1 Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OTg00493
    Clone ID Related Accession (Same CDS sequence) XM_008201341.2
    Accession Version XM_008201341.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1458403200000
    Organism Tribolium castaneum(red flour beetle)
    Product solute carrier family 12 member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008201341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCGGAAC GTTTTCGTGT TTCAAAATCC GAAGATCCAA AAAACATTTT TGTTAAATAT 
    AGTGACTCGC ATTTGTCAGA AGACGAAGAT CATGTGAAAG AAAAGCTCCT GCCATCGAAT 
    AATTCCGAGT ATAAGAAGAC ATCCGGTCAA GGAGATGGCG CCGAAGAGCA ACCTTTTGTG 
    TCGGAAAAGA TGTCGGGGTA CGAGACGAAC TTGTACCTCT ACTCCGAGGA GATGGAGGAC 
    AGACCCAGGA TATCTACCCT CATAAGCTCA TTAGCGAACT ACTCGAACAC CATTCCTCCA 
    GCATCCAATG ATCCCGATGC ACGGCCGGCC GGTGGGGGCG CCCGTATGGG CACCCTCATG 
    GGCGTCTATC TCCCCTGTAT CCAGAACATC TTCGGCGTGA TTCTTTTCAT TCGGTTGACG 
    TGGGTGGTGG GCACAGCAGG GGCGATTCAA GGCTTCCTTA TCGTGTTGTG CTGTTGCTGC 
    GTCACCATGC TGACCGCCAT CTCCATGTCG GCGATCGCCA CCAACGGGGT GGTGCCCGGG 
    GGCGGCAGTT ATTTCATGAT ATCCAGGTCA CTTGGTCCAG AGTTTGGCGG CGCTGTTGGC 
    ATGCTGTTTT ACACAGGGAC GACGTTAGCA GCCGCGATGT ACATCGTCGG TGCTGTTGAA 
    ATTGTCTTGA CCTACATGGC TCCTTGGTTA TCAATTTTTG GGGATTTTAC AACAAATCAG 
    GACGCAATGT ACAACAATTT TCGGGTCTAT GGAACTGGGT TACTCGTGGT GATGGGAACT 
    ATTGTGTTCG TCGGGGTGAA GTTTGTCAAC AAATTTGCCG CCGTTGCTCT TGCATGCGTC 
    ATTCTCTCCA TACTGGCTGT GTACATTGGA ATATTCCGCA ATATCAACGG CAAAGATGAC 
    TTGCAAATGT GCGTTCTTGG TAAACGATTG TTACGTGTCC CGGCTGCTGA TTGTTACAAA 
    AACGGTAGTG GTTTCATACA CAACATGTTT TGTCCTAATG GAACCGCTAG TTGCGATCCG 
    TACTATGAAC GTAATGACGT AAGGCTAGTT CAAGGGATTA AAGGGTTGTC ATCCGGTGTG 
    ATTTTGGACA ATATCTACGA TAGCTTCTTG GAAAATGGAC AGTACATAGC AATAGGGAAT 
    AATCCTGAGG ATATTGAGCC AATGGAACCT CACAGAGACA CTGCCCAAGT GACGGCAGAT 
    ATAACAACAA CTTTTACAAT TTTGATCGGA ATATTTTTCC CTTCAGTGAC TGGTATTATG 
    GCTGGGTCTA ATCGTTCGGG TGATTTAGCT GATGCCCAAA AATCTATACC AATCGGAACA 
    ATTTGTGCCA TTTTAACAAC GTCGACTGTG TATTTATCAT CTGTGATTCT CTTCGCTGCT 
    ACTGTCGACA ACCTTCTACT TAGAGATAAA TTTGGCTCAT CTATAGGAGG TAAATTAGTT 
    GTAGCCAACA TGGCGTGGCC AAACGAATGG GTCATTTTAA TCGGATCATT CCTTTCGACC 
    CTTGGCGCTG GTCTCCAGTC ACTGACTGGA GCTCCTCGCT TACTCCAAGC CATCGCCAAA 
    GACGGTATCA TTCCATTTTT ATCACCGTTT GCCGTATCAT CAAGCCGTGG CGAGCCAACC 
    CGAGCATTAT TATTAACTAT TGTCATTTGT CAGTGTGGTA TATTATTAGG AAATGTTGAT 
    CTTTTGGCGC CCTTACTCTC GATGTTTTTC TTAATGTGCT ACGGTTTTGT AAATCTCGCT 
    TGCGCTTTAC AAACATTACT ACGAACACCC AACTGGAGAC CAAGATTTAA ATATTACCAT 
    TGGTCCCTCT CATTTATCGG CTTATCGTTG TGCATAGCAA TAATGTTTAT GACCTCTTGG 
    TACTTTGCGC TTCTCGCCAT GGGAATGGCA GGCTTGATCT ATAAATACAT CGAATACAGA 
    GGTGCCGAGA AAGAATGGGG TGACGGCATT CGAGGTCTAG CGTTGTCAGC AGCGCGATAT 
    TCATTACTCC GCCTAGAAGA GGGTCCTCCC CACACCAAAA ACTGGAGACC TCAGCTCCTC 
    ATTCTCGTTA AATTAACAAC TGATCTCGTT CCGAAATACA GAAAATTATT TGCTTTCGCG 
    TCGCAACTCA AAGCTGGGAA GGGTTTGACA GTTTGCGCTG CTGTAATTGG CGGCGATTTT 
    ACACGATCGT ACGGAGAAGC GATGGCAGCT AAACAAAGCT TGAGAAAAAC CATGGACGAA 
    GAACGGGTCA AGGGTTTTGT CGATATTCTA GTGGCACGCA ACATTACCGA AGGATTATCT 
    AACTTAGTGC AAACTTCTGG TCTTGGTGGC ATGAAGCCGA ATACGGTGAT TTTAGGATGG 
    CCGTATGGCT GGCGACAGTC GGAAGATGAG CGCACGTGGC AGGTTTTCTT GCAAACTGTT 
    CGGAATGTGA CAGCCGCACA CATGGCGTTA TTAGTTCCTA AAGGTATCAA CTTTTTCCCG 
    GACTCTACGG AGAAAGTCTT CGGGAATATC GATATCTGGT GGATTGTTCA TGACGGTGGT 
    CTTCTCATGT TGTTACCCTT CCTTCTCAAA CAGCACCGCA CTTGGAAAAA TTGCAAGATG 
    CGCATTTTCA CTGTTGCTCA AATGGAAGAC AATTCCATCC AAATGAAGAA AGATCTAAAG 
    ACGTTCTTGT ATCACCTCCG AATTGAAGCA GAAGTTGAAG TCGTTGAAAT GATGGACAGC 
    GATATTTCGG CATACACCTA CGAAAGGACT CTGATGATGG AACAGCGGAA TCAAATGTTG 
    CGAGAACTTC GTCTCAACAA AAAAGAAAGT TTGGGTGTGG TGGATTTGGT GGATTTCAAT 
    GAAATGTCAA CTGAAGAGAA GCTGCCTCTG GTTCAGTCAA TTGTAGATCA ACACCATCAA 
    AATATCGATG CTAAAACAGC CACCAAAGTT AGGTTCCAGG AACCTAGCGC TGAAGGCGAT 
    AAAGATAACA AAGCGGCTGA AACGGAACCG ACAAACGAGC CTAATTCACA GACGAAACCC 
    GAGGATAACG ATAATTCAAC AACAGAGAAA GAAGATCCGT CGGAGAAAGA ATGCAATGAG 
    GAAACAAAAG AGAACAATAT CTCCTCCGAA GAGAAAAAGA AACCCGCAAC GATAACCCCT 
    GACGAAGGCA ACGTCCGTCG GATGCATACA GCTGTAAAAT TAAACGAAGT GATAGTGAAT 
    CGTTCTCACG AAGCTCAGCT TGTGATCTTG AATTTACCCG GCCCTCCAAA AGACACCAAG 
    ATGGAAAGAG AATCCAATTA TATGGAATTC TTGGAAGTGC TCACTGAAGG TTTGGAACGT 
    GTGCTCATGG TCCGTGGTGG CGGTCAAGAG GTCATAACTA TCTATTCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008199563.1
    CDS201..3551
    Translation

    Target ORF information:

    RefSeq Version XM_008201341.2
    Organism Tribolium castaneum(red flour beetle)
    Definition Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_008201341.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCGGAAC GTTTTCGTGT TTCAAAATCC GAAGATCCAA AAAACATTTT TGTTAAATAT 
    AGTGACTCGC ATTTGTCAGA AGACGAAGAT CATGTGAAAG AAAAGCTCCT GCCATCGAAT 
    AATTCCGAGT ATAAGAAGAC ATCCGGTCAA GGAGATGGCG CCGAAGAGCA ACCTTTTGTG 
    TCGGAAAAGA TGTCGGGGTA CGAGACGAAC TTGTACCTCT ACTCCGAGGA GATGGAGGAC 
    AGACCCAGGA TATCTACCCT CATAAGCTCA TTAGCGAACT ACTCGAACAC CATTCCTCCA 
    GCATCCAATG ATCCCGATGC ACGGCCGGCC GGTGGGGGCG CCCGTATGGG CACCCTCATG 
    GGCGTCTATC TCCCCTGTAT CCAGAACATC TTCGGCGTGA TTCTTTTCAT TCGGTTGACG 
    TGGGTGGTGG GCACAGCAGG GGCGATTCAA GGCTTCCTTA TCGTGTTGTG CTGTTGCTGC 
    GTCACCATGC TGACCGCCAT CTCCATGTCG GCGATCGCCA CCAACGGGGT GGTGCCCGGG 
    GGCGGCAGTT ATTTCATGAT ATCCAGGTCA CTTGGTCCAG AGTTTGGCGG CGCTGTTGGC 
    ATGCTGTTTT ACACAGGGAC GACGTTAGCA GCCGCGATGT ACATCGTCGG TGCTGTTGAA 
    ATTGTCTTGA CCTACATGGC TCCTTGGTTA TCAATTTTTG GGGATTTTAC AACAAATCAG 
    GACGCAATGT ACAACAATTT TCGGGTCTAT GGAACTGGGT TACTCGTGGT GATGGGAACT 
    ATTGTGTTCG TCGGGGTGAA GTTTGTCAAC AAATTTGCCG CCGTTGCTCT TGCATGCGTC 
    ATTCTCTCCA TACTGGCTGT GTACATTGGA ATATTCCGCA ATATCAACGG CAAAGATGAC 
    TTGCAAATGT GCGTTCTTGG TAAACGATTG TTACGTGTCC CGGCTGCTGA TTGTTACAAA 
    AACGGTAGTG GTTTCATACA CAACATGTTT TGTCCTAATG GAACCGCTAG TTGCGATCCG 
    TACTATGAAC GTAATGACGT AAGGCTAGTT CAAGGGATTA AAGGGTTGTC ATCCGGTGTG 
    ATTTTGGACA ATATCTACGA TAGCTTCTTG GAAAATGGAC AGTACATAGC AATAGGGAAT 
    AATCCTGAGG ATATTGAGCC AATGGAACCT CACAGAGACA CTGCCCAAGT GACGGCAGAT 
    ATAACAACAA CTTTTACAAT TTTGATCGGA ATATTTTTCC CTTCAGTGAC TGGTATTATG 
    GCTGGGTCTA ATCGTTCGGG TGATTTAGCT GATGCCCAAA AATCTATACC AATCGGAACA 
    ATTTGTGCCA TTTTAACAAC GTCGACTGTG TATTTATCAT CTGTGATTCT CTTCGCTGCT 
    ACTGTCGACA ACCTTCTACT TAGAGATAAA TTTGGCTCAT CTATAGGAGG TAAATTAGTT 
    GTAGCCAACA TGGCGTGGCC AAACGAATGG GTCATTTTAA TCGGATCATT CCTTTCGACC 
    CTTGGCGCTG GTCTCCAGTC ACTGACTGGA GCTCCTCGCT TACTCCAAGC CATCGCCAAA 
    GACGGTATCA TTCCATTTTT ATCACCGTTT GCCGTATCAT CAAGCCGTGG CGAGCCAACC 
    CGAGCATTAT TATTAACTAT TGTCATTTGT CAGTGTGGTA TATTATTAGG AAATGTTGAT 
    CTTTTGGCGC CCTTACTCTC GATGTTTTTC TTAATGTGCT ACGGTTTTGT AAATCTCGCT 
    TGCGCTTTAC AAACATTACT ACGAACACCC AACTGGAGAC CAAGATTTAA ATATTACCAT 
    TGGTCCCTCT CATTTATCGG CTTATCGTTG TGCATAGCAA TAATGTTTAT GACCTCTTGG 
    TACTTTGCGC TTCTCGCCAT GGGAATGGCA GGCTTGATCT ATAAATACAT CGAATACAGA 
    GGTGCCGAGA AAGAATGGGG TGACGGCATT CGAGGTCTAG CGTTGTCAGC AGCGCGATAT 
    TCATTACTCC GCCTAGAAGA GGGTCCTCCC CACACCAAAA ACTGGAGACC TCAGCTCCTC 
    ATTCTCGTTA AATTAACAAC TGATCTCGTT CCGAAATACA GAAAATTATT TGCTTTCGCG 
    TCGCAACTCA AAGCTGGGAA GGGTTTGACA GTTTGCGCTG CTGTAATTGG CGGCGATTTT 
    ACACGATCGT ACGGAGAAGC GATGGCAGCT AAACAAAGCT TGAGAAAAAC CATGGACGAA 
    GAACGGGTCA AGGGTTTTGT CGATATTCTA GTGGCACGCA ACATTACCGA AGGATTATCT 
    AACTTAGTGC AAACTTCTGG TCTTGGTGGC ATGAAGCCGA ATACGGTGAT TTTAGGATGG 
    CCGTATGGCT GGCGACAGTC GGAAGATGAG CGCACGTGGC AGGTTTTCTT GCAAACTGTT 
    CGGAATGTGA CAGCCGCACA CATGGCGTTA TTAGTTCCTA AAGGTATCAA CTTTTTCCCG 
    GACTCTACGG AGAAAGTCTT CGGGAATATC GATATCTGGT GGATTGTTCA TGACGGTGGT 
    CTTCTCATGT TGTTACCCTT CCTTCTCAAA CAGCACCGCA CTTGGAAAAA TTGCAAGATG 
    CGCATTTTCA CTGTTGCTCA AATGGAAGAC AATTCCATCC AAATGAAGAA AGATCTAAAG 
    ACGTTCTTGT ATCACCTCCG AATTGAAGCA GAAGTTGAAG TCGTTGAAAT GATGGACAGC 
    GATATTTCGG CATACACCTA CGAAAGGACT CTGATGATGG AACAGCGGAA TCAAATGTTG 
    CGAGAACTTC GTCTCAACAA AAAAGAAAGT TTGGGTGTGG TGGATTTGGT GGATTTCAAT 
    GAAATGTCAA CTGAAGAGAA GCTGCCTCTG GTTCAGTCAA TTGTAGATCA ACACCATCAA 
    AATATCGATG CTAAAACAGC CACCAAAGTT AGGTTCCAGG AACCTAGCGC TGAAGGCGAT 
    AAAGATAACA AAGCGGCTGA AACGGAACCG ACAAACGAGC CTAATTCACA GACGAAACCC 
    GAGGATAACG ATAATTCAAC AACAGAGAAA GAAGATCCGT CGGAGAAAGA ATGCAATGAG 
    GAAACAAAAG AGAACAATAT CTCCTCCGAA GAGAAAAAGA AACCCGCAAC GATAACCCCT 
    GACGAAGGCA ACGTCCGTCG GATGCATACA GCTGTAAAAT TAAACGAAGT GATAGTGAAT 
    CGTTCTCACG AAGCTCAGCT TGTGATCTTG AATTTACCCG GCCCTCCAAA AGACACCAAG 
    ATGGAAAGAG AATCCAATTA TATGGAATTC TTGGAAGTGC TCACTGAAGG TTTGGAACGT 
    GTGCTCATGG TCCGTGGTGG CGGTCAAGAG GTCATAACTA TCTATTCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC660690 ( XM_008201346.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199568.1 Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OTg00494
    Clone ID Related Accession (Same CDS sequence) XM_008201346.2
    Accession Version XM_008201346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1458403200000
    Organism Tribolium castaneum(red flour beetle)
    Product solute carrier family 12 member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008201346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCGGAAC GTTTTCGTGT TTCAAAATCC GAAGATCCAA AAAACATTTT TGTTAAATAT 
    AGTGACTCGC ATTTGTCAGA AGACGAAGAT CATGTGAAAG AAAAGCTCCT GCCATCGAAT 
    AATTCCGAGT ATAAGAAGAC ATCCGGTCAA GGAGATGGCG CCGAAGAGCA ACCTTTTGTG 
    TCGGAAAAGA TGTCGGGGTA CGAGACGAAC TTGTACCTCT ACTCCGAGGA GATGGAGGAC 
    AGACCCAGGA TATCTACCCT CATAAGCTCA TTAGCGAACT ACTCGAACAC CATTCCTCCA 
    GCATCCAATG ATCCCGATGC ACGGCCGGCC GGTGGGGGCG CCCGTATGGG CACCCTCATG 
    GGCGTCTATC TCCCCTGTAT CCAGAACATC TTCGGCGTGA TTCTTTTCAT TCGGTTGACG 
    TGGGTGGTGG GCACAGCAGG GGCGATTCAA GGCTTCCTTA TCGTGTTGTG CTGTTGCTGC 
    GTCACCATGC TGACCGCCAT CTCCATGTCG GCGATCGCCA CCAACGGGGT GGTGCCCGGG 
    GGCGGCAGTT ATTTCATGAT ATCCAGGTCA CTTGGTCCAG AGTTTGGCGG CGCTGTTGGC 
    ATGCTGTTTT ACACAGGGAC GACGTTAGCA GCCGCGATGT ACATCGTCGG TGCTGTTGAA 
    ATTGTCTTGA CCTACATGGC TCCTTGGTTA TCAATTTTTG GGGATTTTAC AACAAATCAG 
    GACGCAATGT ACAACAATTT TCGGGTCTAT GGAACTGGGT TACTCGTGGT GATGGGAACT 
    ATTGTGTTCG TCGGGGTGAA GTTTGTCAAC AAATTTGCCG CCGTTGCTCT TGCATGCGTC 
    ATTCTCTCCA TACTGGCTGT GTACATTGGA ATATTCCGCA ATATCAACGG CAAAGATGAC 
    TTGCAAATGT GCGTTCTTGG TAAACGATTG TTACGTGTCC CGGCTGCTGA TTGTTACAAA 
    AACGGTAGTG GTTTCATACA CAACATGTTT TGTCCTAATG GAACCGCTAG TTGCGATCCG 
    TACTATGAAC GTAATGACGT AAGGCTAGTT CAAGGGATTA AAGGGTTGTC ATCCGGTGTG 
    ATTTTGGACA ATATCTACGA TAGCTTCTTG GAAAATGGAC AGTACATAGC AATAGGGAAT 
    AATCCTGAGG ATATTGAGCC AATGGAACCT CACAGAGACA CTGCCCAAGT GACGGCAGAT 
    ATAACAACAA CTTTTACAAT TTTGATCGGA ATATTTTTCC CTTCAGTGAC TGGTATTATG 
    GCTGGGTCTA ATCGTTCGGG TGATTTAGCT GATGCCCAAA AATCTATACC AATCGGAACA 
    ATTTGTGCCA TTTTAACAAC GTCGACTGTG TATTTATCAT CTGTGATTCT CTTCGCTGCT 
    ACTGTCGACA ACCTTCTACT TAGAGATAAA TTTGGCTCAT CTATAGGAGG TAAATTAGTT 
    GTAGCCAACA TGGCGTGGCC AAACGAATGG GTCATTTTAA TCGGATCATT CCTTTCGACC 
    CTTGGCGCTG GTCTCCAGTC ACTGACTGGA GCTCCTCGCT TACTCCAAGC CATCGCCAAA 
    GACGGTATCA TTCCATTTTT ATCACCGTTT GCCGTATCAT CAAGCCGTGG CGAGCCAACC 
    CGAGCATTAT TATTAACTAT TGTCATTTGT CAGTGTGGTA TATTATTAGG AAATGTTGAT 
    CTTTTGGCGC CCTTACTCTC GATGTTTTTC TTAATGTGCT ACGGTTTTGT AAATCTCGCT 
    TGCGCTTTAC AAACATTACT ACGAACACCC AACTGGAGAC CAAGATTTAA ATATTACCAT 
    TGGTCCCTCT CATTTATCGG CTTATCGTTG TGCATAGCAA TAATGTTTAT GACCTCTTGG 
    TACTTTGCGC TTCTCGCCAT GGGAATGGCA GGCTTGATCT ATAAATACAT CGAATACAGA 
    GGTGCCGAGA AAGAATGGGG TGACGGCATT CGAGGTCTAG CGTTGTCAGC AGCGCGATAT 
    TCATTACTCC GCCTAGAAGA GGGTCCTCCC CACACCAAAA ACTGGAGACC TCAGCTCCTC 
    ATTCTCGTTA AATTAACAAC TGATCTCGTT CCGAAATACA GAAAATTATT TGCTTTCGCG 
    TCGCAACTCA AAGCTGGGAA GGGTTTGACA GTTTGCGCTG CTGTAATTGG CGGCGATTTT 
    ACACGATCGT ACGGAGAAGC GATGGCAGCT AAACAAAGCT TGAGAAAAAC CATGGACGAA 
    GAACGGGTCA AGGGTTTTGT CGATATTCTA GTGGCACGCA ACATTACCGA AGGATTATCT 
    AACTTAGTGC AAACTTCTGG TCTTGGTGGC ATGAAGCCGA ATACGGTGAT TTTAGGATGG 
    CCGTATGGCT GGCGACAGTC GGAAGATGAG CGCACGTGGC AGGTTTTCTT GCAAACTGTT 
    CGGAATGTGA CAGCCGCACA CATGGCGTTA TTAGTTCCTA AAGGTATCAA CTTTTTCCCG 
    GACTCTACGG AGAAAGTCTT CGGGAATATC GATATCTGGT GGATTGTTCA TGACGGTGGT 
    CTTCTCATGT TGTTACCCTT CCTTCTCAAA CAGCACCGCA CTTGGAAAAA TTGCAAGATG 
    CGCATTTTCA CTGTTGCTCA AATGGAAGAC AATTCCATCC AAATGAAGAA AGATCTAAAG 
    ACGTTCTTGT ATCACCTCCG AATTGAAGCA GAAGTTGAAG TCGTTGAAAT GATGGACAGC 
    GATATTTCGG CATACACCTA CGAAAGGACT CTGATGATGG AACAGCGGAA TCAAATGTTG 
    CGAGAACTTC GTCTCAACAA AAAAGAAAGT TTGGGTGTGG TGGATTTGGT GGATTTCAAT 
    GAAATGTCAA CTGAAGAGAA GCTGCCTCTG GTTCAGTCAA TTGTAGATCA ACACCATCAA 
    AATATCGATG CTAAAACAGC CACCAAAGTT AGGTTCCAGG AACCTAGCGC TGAAGGCGAT 
    AAAGATAACA AAGCGGCTGA AACGGAACCG ACAAACGAGA CGAAACCCGA GGATAACGAT 
    AATTCAACAA CAGAGAAAGA AGATCCGTCG GAGAAAGAAT GCAATGAGGA AACAAAAGAG 
    AACAATATCT CCTCCGAAGA GAAAAAGAAA CCCGCAACGA TAACCCCTGA CGAAGGCAAC 
    GTCCGTCGGA TGCATACAGC TGTAAAATTA AACGAAGTGA TAGTGAATCG TTCTCACGAA 
    GCTCAGCTTG TGATCTTGAA TTTACCCGGC CCTCCAAAAG ACACCAAGAT GGAAAGAGAA 
    TCCAATTATA TGGAATTCTT GGAAGTGCTC ACTGAAGGTT TGGAACGTGT GCTCATGGTC 
    CGTGGTGGCG GTCAAGAGGT CATAACTATC TATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008199568.1
    CDS202..3540
    Translation

    Target ORF information:

    RefSeq Version XM_008201346.2
    Organism Tribolium castaneum(red flour beetle)
    Definition Tribolium castaneum solute carrier family 12 member 4 (LOC660690), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_008201346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCGGAAC GTTTTCGTGT TTCAAAATCC GAAGATCCAA AAAACATTTT TGTTAAATAT 
    AGTGACTCGC ATTTGTCAGA AGACGAAGAT CATGTGAAAG AAAAGCTCCT GCCATCGAAT 
    AATTCCGAGT ATAAGAAGAC ATCCGGTCAA GGAGATGGCG CCGAAGAGCA ACCTTTTGTG 
    TCGGAAAAGA TGTCGGGGTA CGAGACGAAC TTGTACCTCT ACTCCGAGGA GATGGAGGAC 
    AGACCCAGGA TATCTACCCT CATAAGCTCA TTAGCGAACT ACTCGAACAC CATTCCTCCA 
    GCATCCAATG ATCCCGATGC ACGGCCGGCC GGTGGGGGCG CCCGTATGGG CACCCTCATG 
    GGCGTCTATC TCCCCTGTAT CCAGAACATC TTCGGCGTGA TTCTTTTCAT TCGGTTGACG 
    TGGGTGGTGG GCACAGCAGG GGCGATTCAA GGCTTCCTTA TCGTGTTGTG CTGTTGCTGC 
    GTCACCATGC TGACCGCCAT CTCCATGTCG GCGATCGCCA CCAACGGGGT GGTGCCCGGG 
    GGCGGCAGTT ATTTCATGAT ATCCAGGTCA CTTGGTCCAG AGTTTGGCGG CGCTGTTGGC 
    ATGCTGTTTT ACACAGGGAC GACGTTAGCA GCCGCGATGT ACATCGTCGG TGCTGTTGAA 
    ATTGTCTTGA CCTACATGGC TCCTTGGTTA TCAATTTTTG GGGATTTTAC AACAAATCAG 
    GACGCAATGT ACAACAATTT TCGGGTCTAT GGAACTGGGT TACTCGTGGT GATGGGAACT 
    ATTGTGTTCG TCGGGGTGAA GTTTGTCAAC AAATTTGCCG CCGTTGCTCT TGCATGCGTC 
    ATTCTCTCCA TACTGGCTGT GTACATTGGA ATATTCCGCA ATATCAACGG CAAAGATGAC 
    TTGCAAATGT GCGTTCTTGG TAAACGATTG TTACGTGTCC CGGCTGCTGA TTGTTACAAA 
    AACGGTAGTG GTTTCATACA CAACATGTTT TGTCCTAATG GAACCGCTAG TTGCGATCCG 
    TACTATGAAC GTAATGACGT AAGGCTAGTT CAAGGGATTA AAGGGTTGTC ATCCGGTGTG 
    ATTTTGGACA ATATCTACGA TAGCTTCTTG GAAAATGGAC AGTACATAGC AATAGGGAAT 
    AATCCTGAGG ATATTGAGCC AATGGAACCT CACAGAGACA CTGCCCAAGT GACGGCAGAT 
    ATAACAACAA CTTTTACAAT TTTGATCGGA ATATTTTTCC CTTCAGTGAC TGGTATTATG 
    GCTGGGTCTA ATCGTTCGGG TGATTTAGCT GATGCCCAAA AATCTATACC AATCGGAACA 
    ATTTGTGCCA TTTTAACAAC GTCGACTGTG TATTTATCAT CTGTGATTCT CTTCGCTGCT 
    ACTGTCGACA ACCTTCTACT TAGAGATAAA TTTGGCTCAT CTATAGGAGG TAAATTAGTT 
    GTAGCCAACA TGGCGTGGCC AAACGAATGG GTCATTTTAA TCGGATCATT CCTTTCGACC 
    CTTGGCGCTG GTCTCCAGTC ACTGACTGGA GCTCCTCGCT TACTCCAAGC CATCGCCAAA 
    GACGGTATCA TTCCATTTTT ATCACCGTTT GCCGTATCAT CAAGCCGTGG CGAGCCAACC 
    CGAGCATTAT TATTAACTAT TGTCATTTGT CAGTGTGGTA TATTATTAGG AAATGTTGAT 
    CTTTTGGCGC CCTTACTCTC GATGTTTTTC TTAATGTGCT ACGGTTTTGT AAATCTCGCT 
    TGCGCTTTAC AAACATTACT ACGAACACCC AACTGGAGAC CAAGATTTAA ATATTACCAT 
    TGGTCCCTCT CATTTATCGG CTTATCGTTG TGCATAGCAA TAATGTTTAT GACCTCTTGG 
    TACTTTGCGC TTCTCGCCAT GGGAATGGCA GGCTTGATCT ATAAATACAT CGAATACAGA 
    GGTGCCGAGA AAGAATGGGG TGACGGCATT CGAGGTCTAG CGTTGTCAGC AGCGCGATAT 
    TCATTACTCC GCCTAGAAGA GGGTCCTCCC CACACCAAAA ACTGGAGACC TCAGCTCCTC 
    ATTCTCGTTA AATTAACAAC TGATCTCGTT CCGAAATACA GAAAATTATT TGCTTTCGCG 
    TCGCAACTCA AAGCTGGGAA GGGTTTGACA GTTTGCGCTG CTGTAATTGG CGGCGATTTT 
    ACACGATCGT ACGGAGAAGC GATGGCAGCT AAACAAAGCT TGAGAAAAAC CATGGACGAA 
    GAACGGGTCA AGGGTTTTGT CGATATTCTA GTGGCACGCA ACATTACCGA AGGATTATCT 
    AACTTAGTGC AAACTTCTGG TCTTGGTGGC ATGAAGCCGA ATACGGTGAT TTTAGGATGG 
    CCGTATGGCT GGCGACAGTC GGAAGATGAG CGCACGTGGC AGGTTTTCTT GCAAACTGTT 
    CGGAATGTGA CAGCCGCACA CATGGCGTTA TTAGTTCCTA AAGGTATCAA CTTTTTCCCG 
    GACTCTACGG AGAAAGTCTT CGGGAATATC GATATCTGGT GGATTGTTCA TGACGGTGGT 
    CTTCTCATGT TGTTACCCTT CCTTCTCAAA CAGCACCGCA CTTGGAAAAA TTGCAAGATG 
    CGCATTTTCA CTGTTGCTCA AATGGAAGAC AATTCCATCC AAATGAAGAA AGATCTAAAG 
    ACGTTCTTGT ATCACCTCCG AATTGAAGCA GAAGTTGAAG TCGTTGAAAT GATGGACAGC 
    GATATTTCGG CATACACCTA CGAAAGGACT CTGATGATGG AACAGCGGAA TCAAATGTTG 
    CGAGAACTTC GTCTCAACAA AAAAGAAAGT TTGGGTGTGG TGGATTTGGT GGATTTCAAT 
    GAAATGTCAA CTGAAGAGAA GCTGCCTCTG GTTCAGTCAA TTGTAGATCA ACACCATCAA 
    AATATCGATG CTAAAACAGC CACCAAAGTT AGGTTCCAGG AACCTAGCGC TGAAGGCGAT 
    AAAGATAACA AAGCGGCTGA AACGGAACCG ACAAACGAGA CGAAACCCGA GGATAACGAT 
    AATTCAACAA CAGAGAAAGA AGATCCGTCG GAGAAAGAAT GCAATGAGGA AACAAAAGAG 
    AACAATATCT CCTCCGAAGA GAAAAAGAAA CCCGCAACGA TAACCCCTGA CGAAGGCAAC 
    GTCCGTCGGA TGCATACAGC TGTAAAATTA AACGAAGTGA TAGTGAATCG TTCTCACGAA 
    GCTCAGCTTG TGATCTTGAA TTTACCCGGC CCTCCAAAAG ACACCAAGAT GGAAAGAGAA 
    TCCAATTATA TGGAATTCTT GGAAGTGCTC ACTGAAGGTT TGGAACGTGT GCTCATGGTC 
    CGTGGTGGCG GTCAAGAGGT CATAACTATC TATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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gene@genscript.com

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