LOC657455 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC657455
Entrez Gene ID	657455
Full Name	small subunit processome component 20 homolog
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_963915.4	XP_969008.2	small subunit processome component 20 homolog

Pathways from BioSystems
Gene Ontology

The following LOC657455 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC657455 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg15028	XM_963915.4 Latest version!	Tribolium castaneum small subunit processome component 20 homolog (LOC657455), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC657455 ( XM_963915.4 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_969008.2 Tribolium castaneum small subunit processome component 20 homolog (LOC657455), mRNA.

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CloneID	OTg15028
Clone ID Related Accession (Same CDS sequence)	XM_963915.4
Accession Version	XM_963915.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	small subunit processome component 20 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015452276.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_963915.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATA AACCGATTCG TCACAAGGAT TCAAACACAT TTAAGTTCCA ACCCTTTGCG 
GAACGCATTG CTAACATCGA CATAGACATT TTCCACCGAG TGGGGCACAG CAATGAGGCC 
GACGACGAGA CCTCCGAATG CTTTTTCCAC CAAGCGATGG AAAATTGGAG CATTCTTAAC 
CTAACTGAGG GTTTCGATAA TTTCAAGAAG GAAATCAAAG CTCACACTTT AGTCACCCTT 
CCCCAACTTA TTCTGGACAA AGAACGCGTC GTTGAAGTCC TTTTGAAGCA CTTAAGATTG 
CGAAATTTCT TATCGTTACA ACCGCTACTT GATCTTGTCG TAGCAGTTGC GAAAGACTTA 
CGCAAAGACT TTTACCAGTA TTACAAGCCC GTCCTTGAGG TTTTAATCAG TTTATTGAAC 
ACAAAAAACA CGGAACAACT CGAATGGACG TTTACTTGTC TCGCTTATAT TTTAAAATTT 
GTGTGGCGGC CCCTTCTTAA GGACATCACA ACGGTGTTTG ACACCTTTCT CCCACTATTA 
TCAAACTCTC AGCCTGACTA TATTCACAAT TTTGCGGCAA GTAGTTTCGC TTTTATTGCA 
CGAAAAGTCA AAGACTGGCC CACTTTTCTC TCCCACGTCC TCAAATCGGT GAAAAATCGA 
AAAGACGGAG TCGACGGCTG CGGAAAATTA TTTTTTGAGG TGGTTTCGGG CGTCGCAGGC 
CAGTTTCACA GTTGCGCTGA GACAATCCTA CCACTATTTC TGGAAAGCCT CTCAAATGCG 
AAATTCCCCC AAAATATACT TTTCGAAATT TTGGAAAAAG TCGTGGAGAA TATCGTCGAA 
AATATTCACC CGCAAAAGGG GCAACTCTGG TGGGATTGTC TGATCGGCCG ACTTGATTCG 
TTATTAAACA ATCCGGAAAA AAGCGCGAAA GAAATTGAGC TCGTTTTGAG ATTATTGGGT 
CAAAGTATCG AGTACCGAAA CGGCAAATTT TTACAAAACA ATTCCCCCGT AATTAAACTA 
ATTTTTGATA TTTTTAATTG TGATTTGCCC GATTTTGTAC TTCAGACAGC AACAAAAATC 
GCAATTCTAT TATTACTAGC GAAAAACATA AAATTGACAC AAGAACAAGC GTCGGGGATT 
ACCCGAAAAG TCTTAAATTT ACCACAAGAG CAAATTTTGT TATATTTTGT GTCAAACGTT 
TCCGAATTTT CGGCGTTTGA AGCTTTGGTA CTTCCCACAT TTTTGCAACA TTGCGTCAAG 
ACTGGTTTAA ATAATGACAG TTTGAAAGTT TTAACCGAGT TAGTTTTGAA AAAGGCGCCA 
CTTTCTAATT GCGGTATTAA TTTACCTAAA TGGGGGAAAT ACCCTCTCGA TTTCCGGAAT 
CAAACGATTG AAACAAAATT TCTCAACCAT TTGAATTTTA GTGATTTTCA TAATTATGTC 
GCCGCTTTGA TTTGCGTGCC ACATTTGAAT TTGACAGACG TGTCACAGGT GACTGGGATT 
TTAAGCGAAA ATATAAATAA GTTTTTAACA CAAATTGAAA CCGGACAATC AAACATAACT 
AATGTTTTAT TTCTGTTAAA TAGCACAGTC GAGTGTCTCA TTCATTTGAA CGAAATTAGT 
TACTTGAGTG ACAATTTCGA CCGCATTTTT GACACATTAG CGCCAATTTC GAGTTTACTG 
TCATTAAAAA CCATCGATTT TCTCATTACG GTGCTCAAGG ACCGCCCACA CGTCATAAAC 
ATGGCAACGT TGCGTAAAAT CACGGCCAAT CTCGTGTCAG GGTTTAGTTC CCCCCACCAC 
GAAATTCGCC TAGTCACCGC CCACCTCTAT TCCCTCTTCG AGGATTTGCC AGAATTCGAT 
TTAAGACACT CCTCCGATCC CGACACGCCC AAAGAAAAGT TTCAAATTTT TACGATTTGT 
TACAAAATCG AATCAATTGA ATCGCAAGTT CAAACATACA GAGACCAATT GCAAAATTTG 
GAGAAACTAA ATTTTGATAA ACCTCAAATG ATTATGTGCC TCAAGACCGA ATTTCGAACG 
ATTCCGTTGC GGTACTTGTG CGGTACGCTT TATATTAATT TCAAGTTGTT GTGGGAGCCG 
GTGGTTCAAA TAATTGAGGG TCATGCACAC GGATTGGAGA TTGGGGAGTT TTGGGAGGTG 
TTTGGGGCGG AGCTAAGGAC GGTCGTGGGC CGGGTTAGGG ACGAGGGAGA GAAAGGGGAG 
GAGTTTGACG CGAAATGGGA GGTAATTAAT GAGTTGTGGA AAAAGAGTGG GGAGTTCAAG 
TCAGGGCCCG ATTATGCCAA TTATAGACTT TTGTTATGGA AGGCTTTGGG TTTGTTTCCG 
GATATCGCGG AAGCCAAAAC TAGGGACGTG TCAGACTTGC TGCTAGATTT TATCGAAAAG 
GAGTACACTA AAAATAATTC AGCGGTGGCC ACGAATTGGA ATATTAGACA AAATACGAGT 
CAGGAAACCG AAACAGATGA AGGGTTAGAG GACGAAAAAC AGGAAACTAA GCCAAAAAAA 
GGGAAATCGA ACTTGCAGGT TCTGCTCACG TGTCTACCAG TTTTTACTAA AATTCGAAGT 
CCAAAGTCAA TATATCGTGA GCCTGAACTC GCCAAACTCT ATTTTGACTT CCTCCAACAC 
AAAAATTCGC AAGTCCAAAA ACTGGCCTTG GACTGCATCA TGACGTACAA ACACAAATTT 
TTGACACCGT ATAAGGACCA CTTGTATAAC TTGGTCGATG ATAAAAATTT CAAAAATGAG 
TTGGCGGCTT TCCAAATCGA TAAAGAGAGT GGTACTATCC AAGAGGAACA TCGTGACGAT 
TTAGTACCGC TGATTATTCG TATTGTCTTC AGTAAAATGA GTACTAAAAC CGGTTTAAGG 
ACCGGCGGAA AGTCCTCAGG ACAGTTGAGG CGAAATTTAG TGCTCCGGTT TTTGGCCGGT 
TGTCAAGACA AAGAAATGTT GGCTTTCGTC AAAATGGCCT TTCAGTACTA TTCGAAATTT 
TTGGACGATG ATCCTCAAGT TATGGTTAAA GGACTCGAGT CCATAACTTT AGAAAGCTAC 
ATTCCCCCGA AAAGACTACA AAGTGCAATA AACCTCCTCA ATGTTATTCT CGACCAGTTT 
GGTGGTTTGA TGGGAAATGA ACTCCTAACG TTCATACTCC AAATAGTATT AATAATTGGA 
GGCGTCCTCA AAATCGCTTT TGGCCAAATC GGTAACGTCC ATGCAGGCTA TTTGGCCATT 
TTGAGGACTT TGAGGACTGG AGCAATTAAA CTAGTGGAAC GTTTCTTCGG ACAATTCGAG 
CGTTACCAAT GGACTAACAA ACAAATTAAT GCGATTTTCC ACATTTTTGT TTGGCCTTAT 
TTGGACAAAT TGAACGTCGA GGGAATACAT AGTCCCACAA CTTTACTAAA ACTGTTCGTT 
CAATGGGGTT CCAACCCGCG ATATTTCTCT CTTTTGGTCA AACATGAGGA TGGTAGACAA 
GACCAGTATG TTCTTCCGCA CGTTATTCGG TTGTTACTCA ATCCAAAGAG TCACATAACT 
GTGGTTAACG CAATTGAGGA GATGGTCGAG AAGTTGTTGA CGCTACAAGC CGACGAAGAG 
GATTTGCAGC TTGTAATCCC AGTGGATAAC CTCCTACCAG TCCCTCAACA TATCCTCGAT 
CGGATTAAAT TAGACGACAA GTTGAACTAC GGAAGTTGCA TACTTTTACC TTTTGTTTCG 
TCGATTTTGG AAAAGATCCA GAGACGTCTA GATGGGAAAA ACAAGAATTT GAATCAGAGG 
GTACTCTTCA TCTTGTCGTG GATATCAGAG TTGGTGTGGG AAGCTGATAT TAGTGATACC 
ACCCTCAAAT TATTGCTTCC ACTCGCTTTG AAACGTTGCT CGACTCCGAT TGGTGAAGAA 
ATCGTAGTTC AGTTATTGAC AACCATCAGC AATCTAGTTG GCAATGTTGC CAACCCTTGT 
GCACATTTAA AACAAATAAC GCCTTTGTTT GGCGAAGTCA GTTGTGCGTC CGGTCGCAAA 
ATTCTAGTCA AAGTGCTTAA AATATGTTTG AAAGAGTTTG GACCTGATCT GGTTGACGGT 
TTGAACGCTT TTGACACCAA ATGGTTGGAC CAACCCGATT TCCAACGCCG CCATGATGCG 
TTTAAAGAGG TTCACAAAGC GATAGATGAG GGACGAATTG ACTTAGATCT TGGTGTTTTA 
CTCGCTTATA ATTGCTACTA TTTGCTCAAA AGCGAGAAGG ACCTGTCGAT GAAAGAGAAT 
TCGACCCATT GTTTGAAACA ACTGTGCCCC TACTTGATTA ACAAGTATCC AAAACAGGTG 
GATTACATCC TAAATGACAC TATTTTTAAT TTGGTTCGGA ACGGAATGAA GAGCCGAAAT 
GACGATTTCC GGAACGATTG TATCCTGTTA CTAGGGCATT TGGCAAAGGA ATGTCCCGAT 
AGTCACGTGG TGTTGCGCGA TTTGAACAAA TACACCAATA AAAGTGACCC CGAGGTTGAT 
TTTTTCGAAA ATATCACGCA TCTGCAGCTT CACAGACACG CAAGGGCTTT ACTCAAATTT 
TGCTCGATAA CGAAAGATTT GACCAACTTG CCCAATATTC GCACTTTGAC TCAATTTATT 
TTACCGCTGG CTTCGCACTA TTTGTGTAAT GAGAAATACG TGGGTAAAAA CAGTGTCATT 
GACGCGTCGA TTGAGTGCTT AGGAACGATC TGTAGGATCT TGCCATGGCA CCAATACGAA 
GGTCTCTTAA AATTCTATTT GCGGAAGCTT GGGAGAAGCG TCGAGTATCA AAAACAGTTG 
GTTCGGGTGA CTGTGGCCAT TTTGGATGCG TTCCATTATG ATTTGAGCAA GGCGCAAGCT 
GGGGAGGATG TAGCCTTGAC CGCGCCGAAA CCGGAAGTTG AAGATTTAGG ACCGGAAACT 
GGTCTAGAAC ATGAGGATAG GGGTGGCGAT GAGGAAGGGG AACCACAGAA TGAGGATGTA 
GCCCCCAGTG AGGAGAAAAA TCAAAGTGAA GATTTAGACG AGGAACCACT TCTAGATGAT 
GGAGATGCTC TAAAGGATGA GGAAGAGGAC GCTGATGATA ATCAAAGTGT GAAACTTTGT 
GAGAAAGTGA CAGTTTTGTG CAAATCAACA GCTACAAGAG TGACTCGAAG CATCCAAATC 
GTGCTTTTGC CTCAATTGCA TAGATCCTTG GCCGAGTTGA CTCACTATGA CACTTCGCAT 
AAAGTCAACC GCAAGAAAAC AAGTGCCGAA CGCGAGGAGG AAGATTTGCT CCGAGTGCCC 
ATATCTTTGG CTTTGGTTAA GTTGTTGCAA AGATTACCGA AAGCAATACT CGAAGCTAAT 
TTGCCTGGTA TTTTCATGAA GCTTTGTACG TTTTTGAAGT CACACTTGGA GAGTGTCCGG 
CGCGTTACAC GAGAGACGTT ACAAAAAATA ATGCAGACCT TAGGTCCCGA TTATTTGGGG 
TTGCTTTTGG GCGAAATGAC TCCTCTTTTA AACCGTGGTT TTCAAGTCCA TGTCCTCATA 
TTTACAATTC ACGCAGTCCT CAATTGCCTC AAACCCATGT TTGAGGCTTA CCACATCGAT 
AAAGTTCTTC TGACAGTTTT GCAGTTGTGT CAAGCCGACT TGTTTGGGAG CCTTTCCGAG 
GAGAAAGAAG TCGCCAAGAT TGCAGTCAAG ACTTCTGAAG CCAAAAGCAC CAAAAGTTTC 
GACACGTTCC AAATTTTGGC ACAATACATC ACCGACAAGT GCCTCATGGA CCTCATTCTA 
CCAATCAAAA ACGTCCTACA AGCCTCACAC TCGTACAAAA CGGTGTACAA GGCCGAGGAG 
TGCCTCCGTC ACGTGGCTCT AGGTCTGGTC GATAATGAGT TTATCGAAAT CAAGTCACTT 
CTGATTTTTG CTTACGGTAC CGCATCCGAA AGCATTCCCG AACTAGTCGC CCAAACCAAG 
CCCCAGTTGA GCGACAAAGA AAAACAAAAG AAGCAACGCG AGCGTGTTGA TTGCTACATC 
ATTCCCAAAG CACCGACTGG TCGTTCGGGC GTTCGCAACG TCAAGTCGTC ACTAAAAACA 
AACGCGTATC TACTGGTCGA GTTTGGTTTG CGTCTCTGCT ACGTCCTCCT GAAACGCGAG 
AAATTAAAAG ACGAGGCCTT TAACCCGTTC CTTGACCCCT TTATCCCAGT CTTCCTCAAC 
TGTCTCACCT CCAAACACGT CAAGTTGAGC ACTTTGACCC TCCAATGCCT CACGTGGGTC 
ATGAAGTACG ACTTACCCTC TCTCAAAGAC AAAATCGCAA CGATCTCGTC AAGTATTTTC 
GAGATTTTGC ACAAATACGC CTCTGCTGGA CTCAGCAAAG GCGATAATTT CGACCTAGTG 
GTTGCCGCGT TTAAGACCAT GGCGGTGCTT GTGCGTGACG TCAAGTATCA TACTATTGAT 
AAGGAACAAT TGAAGGTTTT GTTACTCTAT GCCGAGCAGG ATATGCACGA TCATGACCGA 
CAAGCTACAG CTTTTAACCT TCTCAAGGCC ATTATCGCCA GGAAGCTAGT CGTGCCGGAG 
ATACACGAAG TGATGGCGAA AGTGGCCGAG TTGAGTATCA CGTCGGAGCT GAGTCACGTG 
AGGGTGCAGG CGAGGGTTGT CTTTCATCAG TATTTGTTGG ATTATCCTTT GGGCCACCAA 
GTGGAAAATC ATGTTTCGTT CTATTTGAAT CAAATGAGCT ATGAGATGCA GTATGGGCGA 
GAGTCGGCCA TTGAGATGAT CAGTACTTTG ATTTCTTCGT TTCCTAAGAA AAATTTGATT 
AAATACTCGG GGACACTTTT CGTGACCTTA GGGGCGCGTC TGGTCAATGA CGAGGCACCG 
GAGTGTCGCA AACAAGTCTC CGAGTGTCTT TCTTCACTCT TGTCTCGATT AGGAAGAAAA 
GACTGTGAGG CATTATACGA TATTGTGCTT CTCTGGCTCA AGGATAAGAA GATAAGTCAT 
CGCCGACTTG CCGCTCAATT ATGCGGAATT TTTGTAACAG TTGAGAAGGC TTTATTCGAG 
TCAAGACTAC CAAGTCTTCT TCCTCTAGTC TTGAAACAGT TCGGATTAAA GGAAGAAGAA 
GATGAAGAAG CGGAACGAAT CAAGGATCAC CACCTATTCC AATTGCTACA AATGTTGCTC 
AAAATTTGTA ACAACTGTCC AAACTTCTTC AAACGTGAAG AAATCGAAGA TTTGAGTAAA 
CACATCCAGG GCTTGCTGGG ATATCCACAC GATTGGGTGA GATTAGCTGC GGCTCAATTC 
CTCGGTTTCG TATTGGCTCA GACCGAGATT GAGGAGTTGG CGCGTTTAGT AGCCAATGGT 
GAGTCAGGGG GGAGTGGCTA CTTGACGCGT GAGCCAATCA CAGCCATCAA AGCACTGACC 
TTGGATTTGT GCGACCAATT ACAACCCGGC AATATCAAGG CGGACTTGGC GGAACAAGTA 
ATCAAGAATC TCGTTTTCGT AGCGCGGGTT TTACAAAAAG TGACAAGTGA CAAAATTAAT 
CTTTTGTGGT TGGTGAAGCG TATGCGGAAA ATCGTAAATA GTGAAATTGT TGAGGGTTCG 
GGTACCGTTT TACGAACCGA AGTTTTCAAA TGGATTGCTG CCGTAGTGAC TGTGCTAGAT 
CTAGACACCA TTACCCCAAT TGTGACTCAT TTGTTGGCGC CTCTAGTGCG CGAAATGGTC 
ACAGTGGAGG AAAGCAACGC CCCCTTGAGA CACCTCGCCA AAGAAGTGGG TACTTTACTC 
AAAAAACGCA TCGGTGTCGA GACCTACACC CAAACCCTGT CCCAGCTCCA ACAGAACTTG 
TCCGTCAAGC GAGCCGAACG TAAGCGTTCG CGAACGCAGT TGGCCGTGAC CGATCCGGAG 
AGCTTCGCTA AAAAGAAGAT CAAGAGACAC GAGAAGAAAA AGGAGGCGAA GAAACGCAAA 
CTGGAGCAGT TGAAAGGCGC GAAAAGACAG TTCAAACGGC GGAAAGCCGT CGATTTGGAT 
CCGGAATCGG AAGTCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_969008.2
CDS	262..8262
Translation	MKNKPIRHKDSNTFKFQPFAERIANIDIDIFHRVGHSNEADDETSECFFHQAMENWSILNLTEGFDNFKKEIKAHTLVTLPQLILDKERVVEVLLKHLRLRNFLSLQPLLDLVVAVAKDLRKDFYQYYKPVLEVLISLLNTKNTEQLEWTFTCLAYILKFVWRPLLKDITTVFDTFLPLLSNSQPDYIHNFAASSFAFIARKVKDWPTFLSHVLKSVKNRKDGVDGCGKLFFEVVSGVAGQFHSCAETILPLFLESLSNAKFPQNILFEILEKVVENIVENIHPQKGQLWWDCLIGRLDSLLNNPEKSAKEIELVLRLLGQSIEYRNGKFLQNNSPVIKLIFDIFNCDLPDFVLQTATKIAILLLLAKNIKLTQEQASGITRKVLNLPQEQILLYFVSNVSEFSAFEALVLPTFLQHCVKTGLNNDSLKVLTELVLKKAPLSNCGINLPKWGKYPLDFRNQTIETKFLNHLNFSDFHNYVAALICVPHLNLTDVSQVTGILSENINKFLTQIETGQSNITNVLFLLNSTVECLIHLNEISYLSDNFDRIFDTLAPISSLLSLKTIDFLITVLKDRPHVINMATLRKITANLVSGFSSPHHEIRLVTAHLYSLFEDLPEFDLRHSSDPDTPKEKFQIFTICYKIESIESQVQTYRDQLQNLEKLNFDKPQMIMCLKTEFRTIPLRYLCGTLYINFKLLWEPVVQIIEGHAHGLEIGEFWEVFGAELRTVVGRVRDEGEKGEEFDAKWEVINELWKKSGEFKSGPDYANYRLLLWKALGLFPDIAEAKTRDVSDLLLDFIEKEYTKNNSAVATNWNIRQNTSQETETDEGLEDEKQETKPKKGKSNLQVLLTCLPVFTKIRSPKSIYREPELAKLYFDFLQHKNSQVQKLALDCIMTYKHKFLTPYKDHLYNLVDDKNFKNELAAFQIDKESGTIQEEHRDDLVPLIIRIVFSKMSTKTGLRTGGKSSGQLRRNLVLRFLAGCQDKEMLAFVKMAFQYYSKFLDDDPQVMVKGLESITLESYIPPKRLQSAINLLNVILDQFGGLMGNELLTFILQIVLIIGGVLKIAFGQIGNVHAGYLAILRTLRTGAIKLVERFFGQFERYQWTNKQINAIFHIFVWPYLDKLNVEGIHSPTTLLKLFVQWGSNPRYFSLLVKHEDGRQDQYVLPHVIRLLLNPKSHITVVNAIEEMVEKLLTLQADEEDLQLVIPVDNLLPVPQHILDRIKLDDKLNYGSCILLPFVSSILEKIQRRLDGKNKNLNQRVLFILSWISELVWEADISDTTLKLLLPLALKRCSTPIGEEIVVQLLTTISNLVGNVANPCAHLKQITPLFGEVSCASGRKILVKVLKICLKEFGPDLVDGLNAFDTKWLDQPDFQRRHDAFKEVHKAIDEGRIDLDLGVLLAYNCYYLLKSEKDLSMKENSTHCLKQLCPYLINKYPKQVDYILNDTIFNLVRNGMKSRNDDFRNDCILLLGHLAKECPDSHVVLRDLNKYTNKSDPEVDFFENITHLQLHRHARALLKFCSITKDLTNLPNIRTLTQFILPLASHYLCNEKYVGKNSVIDASIECLGTICRILPWHQYEGLLKFYLRKLGRSVEYQKQLVRVTVAILDAFHYDLSKAQAGEDVALTAPKPEVEDLGPETGLEHEDRGGDEEGEPQNEDVAPSEEKNQSEDLDEEPLLDDGDALKDEEEDADDNQSVKLCEKVTVLCKSTATRVTRSIQIVLLPQLHRSLAELTHYDTSHKVNRKKTSAEREEEDLLRVPISLALVKLLQRLPKAILEANLPGIFMKLCTFLKSHLESVRRVTRETLQKIMQTLGPDYLGLLLGEMTPLLNRGFQVHVLIFTIHAVLNCLKPMFEAYHIDKVLLTVLQLCQADLFGSLSEEKEVAKIAVKTSEAKSTKSFDTFQILAQYITDKCLMDLILPIKNVLQASHSYKTVYKAEECLRHVALGLVDNEFIEIKSLLIFAYGTASESIPELVAQTKPQLSDKEKQKKQRERVDCYIIPKAPTGRSGVRNVKSSLKTNAYLLVEFGLRLCYVLLKREKLKDEAFNPFLDPFIPVFLNCLTSKHVKLSTLTLQCLTWVMKYDLPSLKDKIATISSSIFEILHKYASAGLSKGDNFDLVVAAFKTMAVLVRDVKYHTIDKEQLKVLLLYAEQDMHDHDRQATAFNLLKAIIARKLVVPEIHEVMAKVAELSITSELSHVRVQARVVFHQYLLDYPLGHQVENHVSFYLNQMSYEMQYGRESAIEMISTLISSFPKKNLIKYSGTLFVTLGARLVNDEAPECRKQVSECLSSLLSRLGRKDCEALYDIVLLWLKDKKISHRRLAAQLCGIFVTVEKALFESRLPSLLPLVLKQFGLKEEEDEEAERIKDHHLFQLLQMLLKICNNCPNFFKREEIEDLSKHIQGLLGYPHDWVRLAAAQFLGFVLAQTEIEELARLVANGESGGSGYLTREPITAIKALTLDLCDQLQPGNIKADLAEQVIKNLVFVARVLQKVTSDKINLLWLVKRMRKIVNSEIVEGSGTVLRTEVFKWIAAVVTVLDLDTITPIVTHLLAPLVREMVTVEESNAPLRHLAKEVGTLLKKRIGVETYTQTLSQLQQNLSVKRAERKRSRTQLAVTDPESFAKKKIKRHEKKKEAKKRKLEQLKGAKRQFKRRKAVDLDPESEVM

Target ORF information:

RefSeq Version	XM_963915.4
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum small subunit processome component 20 homolog (LOC657455), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_963915.4

ORF Insert Sequence:

ATGAAGAATA AACCGATTCG TCACAAGGAT TCAAACACAT TTAAGTTCCA ACCCTTTGCG 
GAACGCATTG CTAACATCGA CATAGACATT TTCCACCGAG TGGGGCACAG CAATGAGGCC 
GACGACGAGA CCTCCGAATG CTTTTTCCAC CAAGCGATGG AAAATTGGAG CATTCTTAAC 
CTAACTGAGG GTTTCGATAA TTTCAAGAAG GAAATCAAAG CTCACACTTT AGTCACCCTT 
CCCCAACTTA TTCTGGACAA AGAACGCGTC GTTGAAGTCC TTTTGAAGCA CTTAAGATTG 
CGAAATTTCT TATCGTTACA ACCGCTACTT GATCTTGTCG TAGCAGTTGC GAAAGACTTA 
CGCAAAGACT TTTACCAGTA TTACAAGCCC GTCCTTGAGG TTTTAATCAG TTTATTGAAC 
ACAAAAAACA CGGAACAACT CGAATGGACG TTTACTTGTC TCGCTTATAT TTTAAAATTT 
GTGTGGCGGC CCCTTCTTAA GGACATCACA ACGGTGTTTG ACACCTTTCT CCCACTATTA 
TCAAACTCTC AGCCTGACTA TATTCACAAT TTTGCGGCAA GTAGTTTCGC TTTTATTGCA 
CGAAAAGTCA AAGACTGGCC CACTTTTCTC TCCCACGTCC TCAAATCGGT GAAAAATCGA 
AAAGACGGAG TCGACGGCTG CGGAAAATTA TTTTTTGAGG TGGTTTCGGG CGTCGCAGGC 
CAGTTTCACA GTTGCGCTGA GACAATCCTA CCACTATTTC TGGAAAGCCT CTCAAATGCG 
AAATTCCCCC AAAATATACT TTTCGAAATT TTGGAAAAAG TCGTGGAGAA TATCGTCGAA 
AATATTCACC CGCAAAAGGG GCAACTCTGG TGGGATTGTC TGATCGGCCG ACTTGATTCG 
TTATTAAACA ATCCGGAAAA AAGCGCGAAA GAAATTGAGC TCGTTTTGAG ATTATTGGGT 
CAAAGTATCG AGTACCGAAA CGGCAAATTT TTACAAAACA ATTCCCCCGT AATTAAACTA 
ATTTTTGATA TTTTTAATTG TGATTTGCCC GATTTTGTAC TTCAGACAGC AACAAAAATC 
GCAATTCTAT TATTACTAGC GAAAAACATA AAATTGACAC AAGAACAAGC GTCGGGGATT 
ACCCGAAAAG TCTTAAATTT ACCACAAGAG CAAATTTTGT TATATTTTGT GTCAAACGTT 
TCCGAATTTT CGGCGTTTGA AGCTTTGGTA CTTCCCACAT TTTTGCAACA TTGCGTCAAG 
ACTGGTTTAA ATAATGACAG TTTGAAAGTT TTAACCGAGT TAGTTTTGAA AAAGGCGCCA 
CTTTCTAATT GCGGTATTAA TTTACCTAAA TGGGGGAAAT ACCCTCTCGA TTTCCGGAAT 
CAAACGATTG AAACAAAATT TCTCAACCAT TTGAATTTTA GTGATTTTCA TAATTATGTC 
GCCGCTTTGA TTTGCGTGCC ACATTTGAAT TTGACAGACG TGTCACAGGT GACTGGGATT 
TTAAGCGAAA ATATAAATAA GTTTTTAACA CAAATTGAAA CCGGACAATC AAACATAACT 
AATGTTTTAT TTCTGTTAAA TAGCACAGTC GAGTGTCTCA TTCATTTGAA CGAAATTAGT 
TACTTGAGTG ACAATTTCGA CCGCATTTTT GACACATTAG CGCCAATTTC GAGTTTACTG 
TCATTAAAAA CCATCGATTT TCTCATTACG GTGCTCAAGG ACCGCCCACA CGTCATAAAC 
ATGGCAACGT TGCGTAAAAT CACGGCCAAT CTCGTGTCAG GGTTTAGTTC CCCCCACCAC 
GAAATTCGCC TAGTCACCGC CCACCTCTAT TCCCTCTTCG AGGATTTGCC AGAATTCGAT 
TTAAGACACT CCTCCGATCC CGACACGCCC AAAGAAAAGT TTCAAATTTT TACGATTTGT 
TACAAAATCG AATCAATTGA ATCGCAAGTT CAAACATACA GAGACCAATT GCAAAATTTG 
GAGAAACTAA ATTTTGATAA ACCTCAAATG ATTATGTGCC TCAAGACCGA ATTTCGAACG 
ATTCCGTTGC GGTACTTGTG CGGTACGCTT TATATTAATT TCAAGTTGTT GTGGGAGCCG 
GTGGTTCAAA TAATTGAGGG TCATGCACAC GGATTGGAGA TTGGGGAGTT TTGGGAGGTG 
TTTGGGGCGG AGCTAAGGAC GGTCGTGGGC CGGGTTAGGG ACGAGGGAGA GAAAGGGGAG 
GAGTTTGACG CGAAATGGGA GGTAATTAAT GAGTTGTGGA AAAAGAGTGG GGAGTTCAAG 
TCAGGGCCCG ATTATGCCAA TTATAGACTT TTGTTATGGA AGGCTTTGGG TTTGTTTCCG 
GATATCGCGG AAGCCAAAAC TAGGGACGTG TCAGACTTGC TGCTAGATTT TATCGAAAAG 
GAGTACACTA AAAATAATTC AGCGGTGGCC ACGAATTGGA ATATTAGACA AAATACGAGT 
CAGGAAACCG AAACAGATGA AGGGTTAGAG GACGAAAAAC AGGAAACTAA GCCAAAAAAA 
GGGAAATCGA ACTTGCAGGT TCTGCTCACG TGTCTACCAG TTTTTACTAA AATTCGAAGT 
CCAAAGTCAA TATATCGTGA GCCTGAACTC GCCAAACTCT ATTTTGACTT CCTCCAACAC 
AAAAATTCGC AAGTCCAAAA ACTGGCCTTG GACTGCATCA TGACGTACAA ACACAAATTT 
TTGACACCGT ATAAGGACCA CTTGTATAAC TTGGTCGATG ATAAAAATTT CAAAAATGAG 
TTGGCGGCTT TCCAAATCGA TAAAGAGAGT GGTACTATCC AAGAGGAACA TCGTGACGAT 
TTAGTACCGC TGATTATTCG TATTGTCTTC AGTAAAATGA GTACTAAAAC CGGTTTAAGG 
ACCGGCGGAA AGTCCTCAGG ACAGTTGAGG CGAAATTTAG TGCTCCGGTT TTTGGCCGGT 
TGTCAAGACA AAGAAATGTT GGCTTTCGTC AAAATGGCCT TTCAGTACTA TTCGAAATTT 
TTGGACGATG ATCCTCAAGT TATGGTTAAA GGACTCGAGT CCATAACTTT AGAAAGCTAC 
ATTCCCCCGA AAAGACTACA AAGTGCAATA AACCTCCTCA ATGTTATTCT CGACCAGTTT 
GGTGGTTTGA TGGGAAATGA ACTCCTAACG TTCATACTCC AAATAGTATT AATAATTGGA 
GGCGTCCTCA AAATCGCTTT TGGCCAAATC GGTAACGTCC ATGCAGGCTA TTTGGCCATT 
TTGAGGACTT TGAGGACTGG AGCAATTAAA CTAGTGGAAC GTTTCTTCGG ACAATTCGAG 
CGTTACCAAT GGACTAACAA ACAAATTAAT GCGATTTTCC ACATTTTTGT TTGGCCTTAT 
TTGGACAAAT TGAACGTCGA GGGAATACAT AGTCCCACAA CTTTACTAAA ACTGTTCGTT 
CAATGGGGTT CCAACCCGCG ATATTTCTCT CTTTTGGTCA AACATGAGGA TGGTAGACAA 
GACCAGTATG TTCTTCCGCA CGTTATTCGG TTGTTACTCA ATCCAAAGAG TCACATAACT 
GTGGTTAACG CAATTGAGGA GATGGTCGAG AAGTTGTTGA CGCTACAAGC CGACGAAGAG 
GATTTGCAGC TTGTAATCCC AGTGGATAAC CTCCTACCAG TCCCTCAACA TATCCTCGAT 
CGGATTAAAT TAGACGACAA GTTGAACTAC GGAAGTTGCA TACTTTTACC TTTTGTTTCG 
TCGATTTTGG AAAAGATCCA GAGACGTCTA GATGGGAAAA ACAAGAATTT GAATCAGAGG 
GTACTCTTCA TCTTGTCGTG GATATCAGAG TTGGTGTGGG AAGCTGATAT TAGTGATACC 
ACCCTCAAAT TATTGCTTCC ACTCGCTTTG AAACGTTGCT CGACTCCGAT TGGTGAAGAA 
ATCGTAGTTC AGTTATTGAC AACCATCAGC AATCTAGTTG GCAATGTTGC CAACCCTTGT 
GCACATTTAA AACAAATAAC GCCTTTGTTT GGCGAAGTCA GTTGTGCGTC CGGTCGCAAA 
ATTCTAGTCA AAGTGCTTAA AATATGTTTG AAAGAGTTTG GACCTGATCT GGTTGACGGT 
TTGAACGCTT TTGACACCAA ATGGTTGGAC CAACCCGATT TCCAACGCCG CCATGATGCG 
TTTAAAGAGG TTCACAAAGC GATAGATGAG GGACGAATTG ACTTAGATCT TGGTGTTTTA 
CTCGCTTATA ATTGCTACTA TTTGCTCAAA AGCGAGAAGG ACCTGTCGAT GAAAGAGAAT 
TCGACCCATT GTTTGAAACA ACTGTGCCCC TACTTGATTA ACAAGTATCC AAAACAGGTG 
GATTACATCC TAAATGACAC TATTTTTAAT TTGGTTCGGA ACGGAATGAA GAGCCGAAAT 
GACGATTTCC GGAACGATTG TATCCTGTTA CTAGGGCATT TGGCAAAGGA ATGTCCCGAT 
AGTCACGTGG TGTTGCGCGA TTTGAACAAA TACACCAATA AAAGTGACCC CGAGGTTGAT 
TTTTTCGAAA ATATCACGCA TCTGCAGCTT CACAGACACG CAAGGGCTTT ACTCAAATTT 
TGCTCGATAA CGAAAGATTT GACCAACTTG CCCAATATTC GCACTTTGAC TCAATTTATT 
TTACCGCTGG CTTCGCACTA TTTGTGTAAT GAGAAATACG TGGGTAAAAA CAGTGTCATT 
GACGCGTCGA TTGAGTGCTT AGGAACGATC TGTAGGATCT TGCCATGGCA CCAATACGAA 
GGTCTCTTAA AATTCTATTT GCGGAAGCTT GGGAGAAGCG TCGAGTATCA AAAACAGTTG 
GTTCGGGTGA CTGTGGCCAT TTTGGATGCG TTCCATTATG ATTTGAGCAA GGCGCAAGCT 
GGGGAGGATG TAGCCTTGAC CGCGCCGAAA CCGGAAGTTG AAGATTTAGG ACCGGAAACT 
GGTCTAGAAC ATGAGGATAG GGGTGGCGAT GAGGAAGGGG AACCACAGAA TGAGGATGTA 
GCCCCCAGTG AGGAGAAAAA TCAAAGTGAA GATTTAGACG AGGAACCACT TCTAGATGAT 
GGAGATGCTC TAAAGGATGA GGAAGAGGAC GCTGATGATA ATCAAAGTGT GAAACTTTGT 
GAGAAAGTGA CAGTTTTGTG CAAATCAACA GCTACAAGAG TGACTCGAAG CATCCAAATC 
GTGCTTTTGC CTCAATTGCA TAGATCCTTG GCCGAGTTGA CTCACTATGA CACTTCGCAT 
AAAGTCAACC GCAAGAAAAC AAGTGCCGAA CGCGAGGAGG AAGATTTGCT CCGAGTGCCC 
ATATCTTTGG CTTTGGTTAA GTTGTTGCAA AGATTACCGA AAGCAATACT CGAAGCTAAT 
TTGCCTGGTA TTTTCATGAA GCTTTGTACG TTTTTGAAGT CACACTTGGA GAGTGTCCGG 
CGCGTTACAC GAGAGACGTT ACAAAAAATA ATGCAGACCT TAGGTCCCGA TTATTTGGGG 
TTGCTTTTGG GCGAAATGAC TCCTCTTTTA AACCGTGGTT TTCAAGTCCA TGTCCTCATA 
TTTACAATTC ACGCAGTCCT CAATTGCCTC AAACCCATGT TTGAGGCTTA CCACATCGAT 
AAAGTTCTTC TGACAGTTTT GCAGTTGTGT CAAGCCGACT TGTTTGGGAG CCTTTCCGAG 
GAGAAAGAAG TCGCCAAGAT TGCAGTCAAG ACTTCTGAAG CCAAAAGCAC CAAAAGTTTC 
GACACGTTCC AAATTTTGGC ACAATACATC ACCGACAAGT GCCTCATGGA CCTCATTCTA 
CCAATCAAAA ACGTCCTACA AGCCTCACAC TCGTACAAAA CGGTGTACAA GGCCGAGGAG 
TGCCTCCGTC ACGTGGCTCT AGGTCTGGTC GATAATGAGT TTATCGAAAT CAAGTCACTT 
CTGATTTTTG CTTACGGTAC CGCATCCGAA AGCATTCCCG AACTAGTCGC CCAAACCAAG 
CCCCAGTTGA GCGACAAAGA AAAACAAAAG AAGCAACGCG AGCGTGTTGA TTGCTACATC 
ATTCCCAAAG CACCGACTGG TCGTTCGGGC GTTCGCAACG TCAAGTCGTC ACTAAAAACA 
AACGCGTATC TACTGGTCGA GTTTGGTTTG CGTCTCTGCT ACGTCCTCCT GAAACGCGAG 
AAATTAAAAG ACGAGGCCTT TAACCCGTTC CTTGACCCCT TTATCCCAGT CTTCCTCAAC 
TGTCTCACCT CCAAACACGT CAAGTTGAGC ACTTTGACCC TCCAATGCCT CACGTGGGTC 
ATGAAGTACG ACTTACCCTC TCTCAAAGAC AAAATCGCAA CGATCTCGTC AAGTATTTTC 
GAGATTTTGC ACAAATACGC CTCTGCTGGA CTCAGCAAAG GCGATAATTT CGACCTAGTG 
GTTGCCGCGT TTAAGACCAT GGCGGTGCTT GTGCGTGACG TCAAGTATCA TACTATTGAT 
AAGGAACAAT TGAAGGTTTT GTTACTCTAT GCCGAGCAGG ATATGCACGA TCATGACCGA 
CAAGCTACAG CTTTTAACCT TCTCAAGGCC ATTATCGCCA GGAAGCTAGT CGTGCCGGAG 
ATACACGAAG TGATGGCGAA AGTGGCCGAG TTGAGTATCA CGTCGGAGCT GAGTCACGTG 
AGGGTGCAGG CGAGGGTTGT CTTTCATCAG TATTTGTTGG ATTATCCTTT GGGCCACCAA 
GTGGAAAATC ATGTTTCGTT CTATTTGAAT CAAATGAGCT ATGAGATGCA GTATGGGCGA 
GAGTCGGCCA TTGAGATGAT CAGTACTTTG ATTTCTTCGT TTCCTAAGAA AAATTTGATT 
AAATACTCGG GGACACTTTT CGTGACCTTA GGGGCGCGTC TGGTCAATGA CGAGGCACCG 
GAGTGTCGCA AACAAGTCTC CGAGTGTCTT TCTTCACTCT TGTCTCGATT AGGAAGAAAA 
GACTGTGAGG CATTATACGA TATTGTGCTT CTCTGGCTCA AGGATAAGAA GATAAGTCAT 
CGCCGACTTG CCGCTCAATT ATGCGGAATT TTTGTAACAG TTGAGAAGGC TTTATTCGAG 
TCAAGACTAC CAAGTCTTCT TCCTCTAGTC TTGAAACAGT TCGGATTAAA GGAAGAAGAA 
GATGAAGAAG CGGAACGAAT CAAGGATCAC CACCTATTCC AATTGCTACA AATGTTGCTC 
AAAATTTGTA ACAACTGTCC AAACTTCTTC AAACGTGAAG AAATCGAAGA TTTGAGTAAA 
CACATCCAGG GCTTGCTGGG ATATCCACAC GATTGGGTGA GATTAGCTGC GGCTCAATTC 
CTCGGTTTCG TATTGGCTCA GACCGAGATT GAGGAGTTGG CGCGTTTAGT AGCCAATGGT 
GAGTCAGGGG GGAGTGGCTA CTTGACGCGT GAGCCAATCA CAGCCATCAA AGCACTGACC 
TTGGATTTGT GCGACCAATT ACAACCCGGC AATATCAAGG CGGACTTGGC GGAACAAGTA 
ATCAAGAATC TCGTTTTCGT AGCGCGGGTT TTACAAAAAG TGACAAGTGA CAAAATTAAT 
CTTTTGTGGT TGGTGAAGCG TATGCGGAAA ATCGTAAATA GTGAAATTGT TGAGGGTTCG 
GGTACCGTTT TACGAACCGA AGTTTTCAAA TGGATTGCTG CCGTAGTGAC TGTGCTAGAT 
CTAGACACCA TTACCCCAAT TGTGACTCAT TTGTTGGCGC CTCTAGTGCG CGAAATGGTC 
ACAGTGGAGG AAAGCAACGC CCCCTTGAGA CACCTCGCCA AAGAAGTGGG TACTTTACTC 
AAAAAACGCA TCGGTGTCGA GACCTACACC CAAACCCTGT CCCAGCTCCA ACAGAACTTG 
TCCGTCAAGC GAGCCGAACG TAAGCGTTCG CGAACGCAGT TGGCCGTGAC CGATCCGGAG 
AGCTTCGCTA AAAAGAAGAT CAAGAGACAC GAGAAGAAAA AGGAGGCGAA GAAACGCAAA 
CTGGAGCAGT TGAAAGGCGC GAAAAGACAG TTCAAACGGC GGAAAGCCGT CGATTTGGAT 
CCGGAATCGG AAGTCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC657455 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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