Para cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	Para
Entrez Gene ID	656403
Full Name	paralytic B
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001165909.1	NP_001159381.1	paralytic B
XM_015982158.1	XP_015837644.1	paralytic B isoform X2
XM_015982185.1	XP_015837671.1	paralytic B isoform X3
XM_015982131.1	XP_015837617.1	paralytic B isoform X1

Pathways from BioSystems
Gene Ontology

The following Para gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Para cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg00684	NM_001165909.1 Latest version!	Tribolium castaneum paralytic B (Para), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16171	XM_015982131.1 Latest version!	Tribolium castaneum paralytic B (Para), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16172	XM_015982158.1 Latest version!	Tribolium castaneum paralytic B (Para), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16173	XM_015982185.1 Latest version!	Tribolium castaneum paralytic B (Para), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Para ( NM_001165909.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001159381.1 Tribolium castaneum paralytic B (Para), mRNA.

Price & Availability↑

CloneID	OTg00684
Clone ID Related Accession (Same CDS sequence)	NM_001165909.1
Accession Version	NM_001165909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1536595200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ202025.1. On Sep 29, 2009 this sequence version replaced XM_962937.2. ##Evidence-Data-START## Transcript exon combination :: GQ202025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02442383, SAMN02442393 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGTTTCTGTT TATTATTTCT CAGCCAATAA TGACGACGAA 
GAGGAAGAGC CGACGTTCAG GGAACGCTTC CTGGCTTATG CCATCAAAAT CATTGACATG 
TTTTGCGTAT GGGATTGTTG TAAATGTTGG GTCGTTATTC AGAAGTTTGT CGCCCTTCTT 
GTCTTCGACC CATTCGTCGA GCTTTTTATC ACGCTGTGCA TTGTTGTTAA CACCTTGTTT 
ATGGCATTGG ATCACCACGA CATGGACAAG GATTTGGAAA AGGCTTTGAA GAGCGGAAAC 
TACTTTTTTA CTGCGACTTT TATGATTGAA GCTACTTTAA AATTGATTGC AATGAGTCCG 
AAATACTACT TTCAAGAAGG CTGGAACATT TTTGATTTTA TTATAGTGGC GTTATCGTTG 
CTGGAGCTAG GACTGGAAGG CGTTCAAGGC TTATCGGTCT TGCGTTCATT TCGACTGTTA 
CGAGTGTTCA AACTGGCCAA GTCGTGGCCA ACTTTAAATT TACTCATTTC CATAATGGGT 
AGAACGATGG GTGCTTTAGG AAACTTGACT TTTGTGCTCT GTATTATAAT ATTTATTTTT 
GCCGTTATGG GCATGCAACT GTTTGGGAAA AATTACACAG ATAACGTGGA TCGGTTTCCC 
GACCACGAGC TCCCCCGCTG GAACTTCACC GACTTCATGC ATTCGTTTAT GATAGTTTTT 
CGCGTTTTGT GTGGCGAATG GATCGAGTCC ATGTGGGACT GTATGCTGGT GGGCGACGTC 
TCTTGCATTC CGTTCTTTCT CGCAACTGTC GTGATTGGCA ATCTTGTGGT CCTTAATCTT 
TTCTTAGCCC TGCTTTTGAG TAACTTTGGA TCTTCGAGTC TGTCGGCGCC GACTGCCGAC 
AATGACACGA ATAAAATAGC AGAGGCTTTT GACAGAATCG GCCGCTTTAT CAGATGGATC 
AAAGGCAATG TTTTACATAT CGCAAAACTG GTCCGATTCA AGCTCACCAA CCAAATTTCC 
GACCAGCCGT CAGGTGAGGG ACCGTCCAAC AGTTGGAACC AAGATGTGAG ATGTGATGGT 
GGTTTAGATA TCTCCGGAGA CGAAATTTTA GCCGACGGTA TAATTAAAAA AAGTCCTAAA 
GACCGACTGG AAGTAACAAT TGGCACCGGA ATGGATCTTA CCGTGCATGG TGATCCCAAA 
AGAGGCAAAA ACAATATTAA CAAAAGCAAA ACAATCGGAA ATTCAATTCT GGAACATGGA 
ATGTACATCG GTCACCTGGA CGAAGACGAA ATTAGTAACA AATCGTACGG TAGTCACAAA 
AATCGATTTA AAGAGGAAAG TCATAAAGGG AGTGTTGATA TGTTAGACGA CCATGAGGAG 
AAACGAGATG CTAGTAAAGA AGAATTAGGA ATTGGTGAAG AAGAAACAGA AGACGACAGA 
TTTGACTTGG AAAAACCACT GACTGACATT GTGATTGTGG GAAACGCTGA CGATATAATT 
ATCGACGATT ACGCAGCCGA CTGCTTTCCC GATTCATGTT ACAAGAAGTT TCCGTTTCTT 
GCAGGCGACG AGGATTCGCC GTTTTGGCAG GGTTGGGGGA ATTTAAGGTC GAAGACTTAT 
AGACTAATAG AGAACAAATA TTTTGAAACG GCCGTAATCA CGATGATTTT GTTGAGTAGC 
ATGGCGTTGG CGTTAGAAGA CGTACATTTG CAAAGTCGGC CGATTTTGCA AGACATACTG 
TATTACATGG ATCGAATATT CACCGTGATA TTCTTTTTCG AGATGTTGAT AAAGTGGTTA 
GCGTTAGGGT TCCAAAAATA CTTTACGAAC GCTTGGTGCT GGCTAGATTT TGTGATTGTA 
ATGGTGTCTC TCATTAATTT TATTGCCTCT CTTTGTGGCG CTGGCGGGAT TCAAGCATTT 
AAAACTATGC GAACGCTGCG GGCTTTGAGA CCACTTCGCG CCATGTCCCG CATGCAAGGG 
ATGAGGGTTG TCGTTAATGC ATTGGTGCAA GCGATACCGT CTATCTTCAA TGTACTGCTA 
GTGTGCTTAA TATTTTGGTT AATTTTTGCT ATAATGGGTG TGCAGTTATT CGCTGGTAAA 
TACTTTAAGT GCGTGGATGC AAACAAAACC ACACTAAGCT ATGAGATTAT CCCTGATGTT 
AACGCGTGTA AAGCTGAGAA TTACACGTGG GAAAACTCCC CAATGAATTT TGATCATGTG 
GGCAAAGCAT ATTTGTGCTT ATTTCAAGTG GCGACATTCA AGGGGTGGAT TCAAATTATG 
AACGATGCTA TAGATTCTAG AGACATCAAC AAACAACCAA TAAGAGAAAC AAACATTTAT 
ATGTATTTAT ACTTCGTATT TTTTATAATA TTTGGATCGT TTTTTACACT TAACTTATTC 
ATTGGTGTAA TCATCGATAA TTTTAACGAA CAAAAGAAAA AGGGTGGCGA TTCATTGGCA 
CTGATGATGA CAGACGCCCA GAAGAAATAT TACAATGCGA TGAAAAAAAT GGGCTCTAAG 
AAACCAATGA AGGCGATACC GAGGCCGAGG TGGAGACCTC AAGCTATAGT TTTCGAAATA 
GTAACAAATA AGAAATTTGA TATGATAATC ATGTTGTTTA TTGGACTGAA CATGCTTACG 
ATGACAATGG ATCACTACCA ACAGAAAGAA ACTTTCACGA AAGTCCTCGA TTCCCTCAAC 
ATGATTTTCA TTGTTATCTT TAGTACCGAG TGCCTCATGA AAATTTTTGC GTTACGTTAC 
CACTACTTTA CGGAACCTTG GAACCTGTTC GATTTAGTGG TAGTAATATT ATCAATTTTG 
GGTTTGGTTT TGAGTGACAT CATAGAGAAA TATTTCGTGT CACCGACGTT GTTAAGGGTG 
GTTCGTGTGG CCAAAGTAGG GCGAGTTCTG CGTTTGGTGA AAGGTGCAAA GGGCATTCGG 
ACACTGCTGT TCGCACTAGC CATGTCACTG CCGGCACTGT TCAACATCTG TTTGTTGCTG 
TTCCTGGTCA TGTTCATTTT TGCCATCTTC GGGATGTCGT TCTTCATGCA CGTGAAGGAC 
AAGAGCGGAC TTGACGATGT CTACAACTTT AAAACGTTTG GCCAGTCGAT GATTCTCCTC 
TTCCAGATGT CCACTTCGGC GGGCTGGGAT GGAGTCCTGG ATGGTATTAT CAATGAAGAA 
GATTGTAAAC CGCCAAACAG TGAGATCGGC GAGACCGGAA ATTGCGGGAA TTCGACAATT 
GGTATCGCAT TCCTGCTCAG CTATCTCGTG ATTTCCTTCC TCATCGTCAT CAACATGTAC 
ATCGCTGTGA TTTTGGAGAA CTACTCCCAG GCGACGGAGG ATGTGCAAGA GGGTCTGACC 
GATGATGACT ACGACATGTA CTACGAGATC TGGCAGCAGT TCGACCCTGA CGGCACCCAG 
TACATCCGCT ACGACCAACT GTCGGACTTG TTCGATGTGT TGGAGCCTCC GCTACAGATC 
CACAAGCCCA ACAAGTTCAA AATCTACTCC ATGGACATTC CGATCTGCAA GGGTGACATG 
ATGTTTTGTG TGGACATTTT GGACGCGCTA ACAAAAGACT TCTTCGCTCG CAAGGGCAAC 
GAAATCGAGG AAACTGCTGA ACTTAGTGAA GTGCAACCGA GACAGAACGA ACCGGGTTAC 
GAACAAGTCA GTTCGACGCT TTACAGACAG CGCGAAGAAT ATTGTGCGAG ACTGATCCAG 
AATGCCTGGC GCAAGTATAG ACAGCGCACT GGCGGTGCTC CAGAGCAAGT GAATGTCGAT 
GGGGCTGCCG GCGAGGGCGA TGGGGAGGTG GAGGTCCACC AAACGGCCGT TCTGGTCGAG 
AGCGACGGCT TTGTGACCAA GAACGGGCAC AAGGTTGTGC TGCACAGTCG CACACCCAGC 
ATCAGCTCCA AGTCTGCGGA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159381.1
CDS	1..6147
Translation	MSVASDYSSEEELSLFRPFTRESLAVIETRIAEEYAKQKELEKKRAEGETGFGRRKKKKDIRYEDEDEDEGPQPDPTLEQGLPLPVRLHSTFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALIGLQIYMGVLTQKCIKEFPLDGSWGNLTHENWERFVSNESNWYWDDEKGERPLCGNSSGSGMCKEGYVCLQGYGDNPNYGYTSFDTFGWALLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEANAIREAEQKAKDKQDRADARAAAAVEAAEAAAAAAAAAAAGCDNDQYIIKSSSDYSCHSYESFVGQPNGQDDNNKEKISIRSEGLDSVSEQRRVPTNLSKMRKVSAASLSLPGSPFNLRRGSRGSHQFTLRNNRRMVAPPGDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGVMVLPMYYTNLGSRHSSYTSHASRMSYTSHGDLLGGLGSNGKMMTKESQLRNRSLRNGPAAAPLNHKSTRGVYDGPTGHICESKVKQIDNPFIDSNQRQTVVDMKDVMVLNDIIEQAAVSGGSDRGVSVYYFSANNDDEEEEPTFRERFLAYAIKIIDMFCVWDCCKCWVVIQKFVALLVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATLKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFDRIGRFIRWIKGNVLHIAKLVRFKLTNQISDQPSGEGPSNSWNQDVRCDGGLDISGDEILADGIIKKSPKDRLEVTIGTGMDLTVHGDPKRGKNNINKSKTIGNSILEHGMYIGHLDEDEISNKSYGSHKNRFKEESHKGSVDMLDDHEEKRDASKEELGIGEEETEDDRFDLEKPLTDIVIVGNADDIIIDDYAADCFPDSCYKKFPFLAGDEDSPFWQGWGNLRSKTYRLIENKYFETAVITMILLSSMALALEDVHLQSRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFVIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTTLSYEIIPDVNACKAENYTWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKGGDSLALMMTDAQKKYYNAMKKMGSKKPMKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTKVLDSLNMIFIVIFSTECLMKIFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCKPPNSEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDLFDVLEPPLQIHKPNKFKIYSMDIPICKGDMMFCVDILDALTKDFFARKGNEIEETAELSEVQPRQNEPGYEQVSSTLYRQREEYCARLIQNAWRKYRQRTGGAPEQVNVDGAAGEGDGEVEVHQTAVLVESDGFVTKNGHKVVLHSRTPSISSKSADV

Target ORF information:

RefSeq Version	NM_001165909.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic B (Para), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165909.1

ORF Insert Sequence:

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGTTTCTGTT TATTATTTCT CAGCCAATAA TGACGACGAA 
GAGGAAGAGC CGACGTTCAG GGAACGCTTC CTGGCTTATG CCATCAAAAT CATTGACATG 
TTTTGCGTAT GGGATTGTTG TAAATGTTGG GTCGTTATTC AGAAGTTTGT CGCCCTTCTT 
GTCTTCGACC CATTCGTCGA GCTTTTTATC ACGCTGTGCA TTGTTGTTAA CACCTTGTTT 
ATGGCATTGG ATCACCACGA CATGGACAAG GATTTGGAAA AGGCTTTGAA GAGCGGAAAC 
TACTTTTTTA CTGCGACTTT TATGATTGAA GCTACTTTAA AATTGATTGC AATGAGTCCG 
AAATACTACT TTCAAGAAGG CTGGAACATT TTTGATTTTA TTATAGTGGC GTTATCGTTG 
CTGGAGCTAG GACTGGAAGG CGTTCAAGGC TTATCGGTCT TGCGTTCATT TCGACTGTTA 
CGAGTGTTCA AACTGGCCAA GTCGTGGCCA ACTTTAAATT TACTCATTTC CATAATGGGT 
AGAACGATGG GTGCTTTAGG AAACTTGACT TTTGTGCTCT GTATTATAAT ATTTATTTTT 
GCCGTTATGG GCATGCAACT GTTTGGGAAA AATTACACAG ATAACGTGGA TCGGTTTCCC 
GACCACGAGC TCCCCCGCTG GAACTTCACC GACTTCATGC ATTCGTTTAT GATAGTTTTT 
CGCGTTTTGT GTGGCGAATG GATCGAGTCC ATGTGGGACT GTATGCTGGT GGGCGACGTC 
TCTTGCATTC CGTTCTTTCT CGCAACTGTC GTGATTGGCA ATCTTGTGGT CCTTAATCTT 
TTCTTAGCCC TGCTTTTGAG TAACTTTGGA TCTTCGAGTC TGTCGGCGCC GACTGCCGAC 
AATGACACGA ATAAAATAGC AGAGGCTTTT GACAGAATCG GCCGCTTTAT CAGATGGATC 
AAAGGCAATG TTTTACATAT CGCAAAACTG GTCCGATTCA AGCTCACCAA CCAAATTTCC 
GACCAGCCGT CAGGTGAGGG ACCGTCCAAC AGTTGGAACC AAGATGTGAG ATGTGATGGT 
GGTTTAGATA TCTCCGGAGA CGAAATTTTA GCCGACGGTA TAATTAAAAA AAGTCCTAAA 
GACCGACTGG AAGTAACAAT TGGCACCGGA ATGGATCTTA CCGTGCATGG TGATCCCAAA 
AGAGGCAAAA ACAATATTAA CAAAAGCAAA ACAATCGGAA ATTCAATTCT GGAACATGGA 
ATGTACATCG GTCACCTGGA CGAAGACGAA ATTAGTAACA AATCGTACGG TAGTCACAAA 
AATCGATTTA AAGAGGAAAG TCATAAAGGG AGTGTTGATA TGTTAGACGA CCATGAGGAG 
AAACGAGATG CTAGTAAAGA AGAATTAGGA ATTGGTGAAG AAGAAACAGA AGACGACAGA 
TTTGACTTGG AAAAACCACT GACTGACATT GTGATTGTGG GAAACGCTGA CGATATAATT 
ATCGACGATT ACGCAGCCGA CTGCTTTCCC GATTCATGTT ACAAGAAGTT TCCGTTTCTT 
GCAGGCGACG AGGATTCGCC GTTTTGGCAG GGTTGGGGGA ATTTAAGGTC GAAGACTTAT 
AGACTAATAG AGAACAAATA TTTTGAAACG GCCGTAATCA CGATGATTTT GTTGAGTAGC 
ATGGCGTTGG CGTTAGAAGA CGTACATTTG CAAAGTCGGC CGATTTTGCA AGACATACTG 
TATTACATGG ATCGAATATT CACCGTGATA TTCTTTTTCG AGATGTTGAT AAAGTGGTTA 
GCGTTAGGGT TCCAAAAATA CTTTACGAAC GCTTGGTGCT GGCTAGATTT TGTGATTGTA 
ATGGTGTCTC TCATTAATTT TATTGCCTCT CTTTGTGGCG CTGGCGGGAT TCAAGCATTT 
AAAACTATGC GAACGCTGCG GGCTTTGAGA CCACTTCGCG CCATGTCCCG CATGCAAGGG 
ATGAGGGTTG TCGTTAATGC ATTGGTGCAA GCGATACCGT CTATCTTCAA TGTACTGCTA 
GTGTGCTTAA TATTTTGGTT AATTTTTGCT ATAATGGGTG TGCAGTTATT CGCTGGTAAA 
TACTTTAAGT GCGTGGATGC AAACAAAACC ACACTAAGCT ATGAGATTAT CCCTGATGTT 
AACGCGTGTA AAGCTGAGAA TTACACGTGG GAAAACTCCC CAATGAATTT TGATCATGTG 
GGCAAAGCAT ATTTGTGCTT ATTTCAAGTG GCGACATTCA AGGGGTGGAT TCAAATTATG 
AACGATGCTA TAGATTCTAG AGACATCAAC AAACAACCAA TAAGAGAAAC AAACATTTAT 
ATGTATTTAT ACTTCGTATT TTTTATAATA TTTGGATCGT TTTTTACACT TAACTTATTC 
ATTGGTGTAA TCATCGATAA TTTTAACGAA CAAAAGAAAA AGGGTGGCGA TTCATTGGCA 
CTGATGATGA CAGACGCCCA GAAGAAATAT TACAATGCGA TGAAAAAAAT GGGCTCTAAG 
AAACCAATGA AGGCGATACC GAGGCCGAGG TGGAGACCTC AAGCTATAGT TTTCGAAATA 
GTAACAAATA AGAAATTTGA TATGATAATC ATGTTGTTTA TTGGACTGAA CATGCTTACG 
ATGACAATGG ATCACTACCA ACAGAAAGAA ACTTTCACGA AAGTCCTCGA TTCCCTCAAC 
ATGATTTTCA TTGTTATCTT TAGTACCGAG TGCCTCATGA AAATTTTTGC GTTACGTTAC 
CACTACTTTA CGGAACCTTG GAACCTGTTC GATTTAGTGG TAGTAATATT ATCAATTTTG 
GGTTTGGTTT TGAGTGACAT CATAGAGAAA TATTTCGTGT CACCGACGTT GTTAAGGGTG 
GTTCGTGTGG CCAAAGTAGG GCGAGTTCTG CGTTTGGTGA AAGGTGCAAA GGGCATTCGG 
ACACTGCTGT TCGCACTAGC CATGTCACTG CCGGCACTGT TCAACATCTG TTTGTTGCTG 
TTCCTGGTCA TGTTCATTTT TGCCATCTTC GGGATGTCGT TCTTCATGCA CGTGAAGGAC 
AAGAGCGGAC TTGACGATGT CTACAACTTT AAAACGTTTG GCCAGTCGAT GATTCTCCTC 
TTCCAGATGT CCACTTCGGC GGGCTGGGAT GGAGTCCTGG ATGGTATTAT CAATGAAGAA 
GATTGTAAAC CGCCAAACAG TGAGATCGGC GAGACCGGAA ATTGCGGGAA TTCGACAATT 
GGTATCGCAT TCCTGCTCAG CTATCTCGTG ATTTCCTTCC TCATCGTCAT CAACATGTAC 
ATCGCTGTGA TTTTGGAGAA CTACTCCCAG GCGACGGAGG ATGTGCAAGA GGGTCTGACC 
GATGATGACT ACGACATGTA CTACGAGATC TGGCAGCAGT TCGACCCTGA CGGCACCCAG 
TACATCCGCT ACGACCAACT GTCGGACTTG TTCGATGTGT TGGAGCCTCC GCTACAGATC 
CACAAGCCCA ACAAGTTCAA AATCTACTCC ATGGACATTC CGATCTGCAA GGGTGACATG 
ATGTTTTGTG TGGACATTTT GGACGCGCTA ACAAAAGACT TCTTCGCTCG CAAGGGCAAC 
GAAATCGAGG AAACTGCTGA ACTTAGTGAA GTGCAACCGA GACAGAACGA ACCGGGTTAC 
GAACAAGTCA GTTCGACGCT TTACAGACAG CGCGAAGAAT ATTGTGCGAG ACTGATCCAG 
AATGCCTGGC GCAAGTATAG ACAGCGCACT GGCGGTGCTC CAGAGCAAGT GAATGTCGAT 
GGGGCTGCCG GCGAGGGCGA TGGGGAGGTG GAGGTCCACC AAACGGCCGT TCTGGTCGAG 
AGCGACGGCT TTGTGACCAA GAACGGGCAC AAGGTTGTGC TGCACAGTCG CACACCCAGC 
ATCAGCTCCA AGTCTGCGGA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015982131.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837617.1 Tribolium castaneum paralytic B (Para), transcript variant X1, mRNA.

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CloneID	OTg16171
Clone ID Related Accession (Same CDS sequence)	XM_015982131.1
Accession Version	XM_015982131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGCCAATAAT GACGACGAAG AGGAAGAGCC GACGTTCAGG 
GAACGCTTCC TGGCTTATGC CATCAAAATC ATTGACATGT TTTGCGTATG GGATTGTTGT 
AAATGTTGGG TCGTTATTCA GAAGTTTGTC GCCCTTCTTG TCTTCGACCC ATTCGTCGAG 
CTTTTTATCA CGCTGTGCAT TGTTGTTAAC ACCTTGTTTA TGGCATTGGA TCACCACGAC 
ATGGACAAGG ATTTGGAAAA GGCTTTGAAG AGCGGAAACT ACTTTTTTAC TGCGACTTTT 
ATGATTGAAG CTACTTTAAA ATTGATTGCA ATGAGTCCGA AATACTACTT TCAAGAAGGC 
TGGAACATTT TTGATTTTAT TATAGTGGCG TTATCGTTGC TGGAGCTAGG ACTGGAAGGC 
GTTCAAGGCT TATCGGTCTT GCGTTCATTT CGACTGTTAC GAGTGTTCAA ACTGGCCAAG 
TCGTGGCCAA CTTTAAATTT ACTCATTTCC ATAATGGGTA GAACGATGGG TGCTTTAGGA 
AACTTGACTT TTGTGCTCTG TATTATAATA TTTATTTTTG CCGTTATGGG CATGCAACTG 
TTTGGGAAAA ATTACACAGA TAACGTGGAT CGGTTTCCCG ACCACGAGCT CCCCCGCTGG 
AACTTCACCG ACTTCATGCA TTCGTTTATG ATAGTTTTTC GCGTTTTGTG TGGCGAATGG 
ATCGAGTCCA TGTGGGACTG TATGCTGGTG GGCGACGTCT CTTGCATTCC GTTCTTTCTC 
GCAACTGTCG TGATTGGCAA TCTTGTGGTC CTTAATCTTT TCTTAGCCCT GCTTTTGAGT 
AACTTTGGAT CTTCGAGTCT GTCGGCGCCG ACTGCCGACA ATGACACGAA TAAAATAGCA 
GAGGCTTTTG ACAGAATCGG CCGCTTTATC AGATGGATCA AAGGCAATGT TTTACATATC 
GCAAAACTGG TCCGATTCAA GCTCACCAAC CAAATTTCCG ACCAGCCGTC AGGTGAGGGA 
CCGTCCAACA GTTGGAACCA AGATGTGAGA TGTGATGGTG GTTTAGATAT CTCCGGAGAC 
GAAATTTTAG CCGACGGTAT AATTAAAAAA AGTCCTAAAG ACCGACTGGA AGTAACAATT 
GGCACCGGAA TGGATCTTAC CGTGCATGGT GATCCCAAAA GAGGCAAAAA CAATATTAAC 
AAAAGCAAAA CAATCGGAAA TTCAATTCTG GAACATGGAA TGTACATCGG TCACCTGGAC 
GAAGACGAAA TTAGTAACAA ATCGTACGGT AGTCACAAAA ATCGATTTAA AGAGGAAAGT 
CATAAAGGGA GTGTTGATAT GTTAGACGAC CATGAGGAGA AACGAGATGC TAGTAAAGAA 
GAATTAGGAA TTGGTGAAGA AGAAACAGAA GACGACAGAT TTGACTTGGA AAAACCACTG 
ACTGACATTG TGATTGTGGG AAACGCTGAC GATATAATTA TCGACGATTA CGCAGCCGAC 
TGCTTTCCCG ATTCATGTTA CAAGAAGTTT CCGTTTCTTG CAGGCGACGA GGATTCGCCG 
TTTTGGCAGG GTTGGGGGAA TTTAAGGTCG AAGACTTATA GACTAATAGA GAACAAATAT 
TTTGAAACGG CCGTAATCAC GATGATTTTG TTGAGTAGCA TGGCGTTGGC GTTAGAAGAC 
GTACATTTGC AAAGTCGGCC GATTTTGCAA GACATACTGT ATTACATGGA TCGAATATTC 
ACCGTGATAT TCTTTTTCGA GATGTTGATA AAGTGGTTAG CGTTAGGGTT CCAAAAATAC 
TTTACGAACG CTTGGTGCTG GCTAGATTTT GTGATTGTAA TGGTGTCTCT CATTAATTTT 
ATTGCCTCTC TTTGTGGCGC TGGCGGGATT CAAGCATTTA AAACTATGCG AACGCTGCGG 
GCTTTGAGAC CACTTCGCGC CATGTCCCGC ATGCAAGGGA TGAGGGTTGT CGTTAATGCA 
TTGGTGCAAG CGATACCGTC TATCTTCAAT GTACTGCTAG TGTGCTTAAT ATTTTGGTTA 
ATTTTTGCTA TAATGGGTGT GCAGTTATTC GCTGGTAAAT ACTTTAAGTG CGTGGATGCA 
AACAAAACCA CACTAAGCTA TGAGATTATC CCTGATGTTA ACGCGTGTAA AGCTGAGAAT 
TACACGTGGG AAAACTCCCC AATGAATTTT GATCATGTGG GCAAAGCATA TTTGTGCTTA 
TTTCAAGTGG CGACATTCAA GGGGTGGATT CAAATTATGA ACGATGCTAT AGATTCTAGA 
GACATCAACA AACAACCAAT AAGAGAAACA AACATTTATA TGTATTTATA CTTCGTATTT 
TTTATAATAT TTGGATCGTT TTTTACACTT AACTTATTCA TTGGTGTAAT CATCGATAAT 
TTTAACGAAC AAAAGAAAAA GGGTGGCGAT TCATTGGCAC TGATGATGAC AGACGCCCAG 
AAGAAATATT ACAATGCGAT GAAAAAAATG GGCTCTAAGA AACCAATGAA GGCGATACCG 
AGGCCGAGGT GGAGACCTCA AGCTATAGTT TTCGAAATAG TAACAAATAA GAAATTTGAT 
ATGATAATCA TGTTGTTTAT TGGACTGAAC ATGCTTACGA TGACAATGGA TCACTACCAA 
CAGAAAGAAA CTTTCACGAA AGTCCTCGAT TCCCTCAACA TGATTTTCAT TGTTATCTTT 
AGTACCGAGT GCCTCATGAA AATTTTTGCG TTACGTTACC ACTACTTTAC GGAACCTTGG 
AACCTGTTCG ATTTAGTGGT AGTAATATTA TCAATTTTGG GTTTGGTTTT GAGTGACATC 
ATAGAGAAAT ATTTCGTGTC ACCGACGTTG TTAAGGGTGG TTCGTGTGGC CAAAGTAGGG 
CGAGTTCTGC GTTTGGTGAA AGGTGCAAAG GGCATTCGGA CACTGCTGTT CGCACTAGCC 
ATGTCACTGC CGGCACTGTT CAACATCTGT TTGTTGCTGT TCCTGGTCAT GTTCATTTTT 
GCCATCTTCG GGATGTCGTT CTTCATGCAC GTGAAGGACA AGAGCGGACT TGACGATGTC 
TACAACTTTA AAACGTTTGG CCAGTCGATG ATTCTCCTCT TCCAGATGTC CACTTCGGCG 
GGCTGGGATG GAGTCCTGGA TGGTATTATC AATGAAGAAG ATTGTAAACC GCCAAACAGT 
GAGATCGGCG AGACCGGAAA TTGCGGGAAT TCGACAATTG GTATCGCATT CCTGCTCAGC 
TATCTCGTGA TTTCCTTCCT CATCGTCATC AACATGTACA TCGCTGTGAT TTTGGAGAAC 
TACTCCCAGG CGACGGAGGA TGTGCAAGAG GGTCTGACCG ATGATGACTA CGACATGTAC 
TACGAGATCT GGCAGCAGTT CGACCCTGAC GGCACCCAGT ACATCCGCTA CGACCAACTG 
TCGGACTTGT TCGATGTGTT GGAGCCTCCG CTACAGATCC ACAAGCCCAA CAAGTTCAAA 
ATCTACTCCA TGGACATTCC GATCTGCAAG GGTGACATGA TGTTTTGTGT GGACATTTTG 
GACGCGCTAA CAAAAGACTT CTTCGCTCGC AAGGGCAACG AAATCGAGGA AACTGCTGAA 
CTTAGTGAAG TGCAACCGAG ACAGAACGAA CCGGGTTACG AACAAGTCAG TTCGACGCTT 
TACAGACAGC GCGAAGAATA TTGTGCGAGA CTGATCCAGA ATGCCTGGCG CAAGTATAGA 
CAGCGCACTG GCGGTGCTCC AGAGCAAGTG AATGTCGATG GGGCTGCCGG CGAGGGCGAT 
GGGGAGGTGG AGGTCCACCA AACGGCCGTT CTGGTCGAGA GCGACGGCTT TGTGACCAAG 
AACGGGCACA AGGTTGTGCT GCACAGTCGC ACACCCAGCA TCAGCTCCAA GTCTGCGGAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837617.1
CDS	1..6126
Translation	MSVASDYSSEEELSLFRPFTRESLAVIETRIAEEYAKQKELEKKRAEGETGFGRRKKKKDIRYEDEDEDEGPQPDPTLEQGLPLPVRLHSTFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALIGLQIYMGVLTQKCIKEFPLDGSWGNLTHENWERFVSNESNWYWDDEKGERPLCGNSSGSGMCKEGYVCLQGYGDNPNYGYTSFDTFGWALLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEANAIREAEQKAKDKQDRADARAAAAVEAAEAAAAAAAAAAAGCDNDQYIIKSSSDYSCHSYESFVGQPNGQDDNNKEKISIRSEGLDSVSEQRRVPTNLSKMRKVSAASLSLPGSPFNLRRGSRGSHQFTLRNNRRMVAPPGDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGVMVLPMYYTNLGSRHSSYTSHASRMSYTSHGDLLGGLGSNGKMMTKESQLRNRSLRNGPAAAPLNHKSTRGVYDGPTGHICESKVKQIDNPFIDSNQRQTVVDMKDVMVLNDIIEQAAVSGGSDRGANNDDEEEEPTFRERFLAYAIKIIDMFCVWDCCKCWVVIQKFVALLVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATLKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFDRIGRFIRWIKGNVLHIAKLVRFKLTNQISDQPSGEGPSNSWNQDVRCDGGLDISGDEILADGIIKKSPKDRLEVTIGTGMDLTVHGDPKRGKNNINKSKTIGNSILEHGMYIGHLDEDEISNKSYGSHKNRFKEESHKGSVDMLDDHEEKRDASKEELGIGEEETEDDRFDLEKPLTDIVIVGNADDIIIDDYAADCFPDSCYKKFPFLAGDEDSPFWQGWGNLRSKTYRLIENKYFETAVITMILLSSMALALEDVHLQSRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFVIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTTLSYEIIPDVNACKAENYTWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKGGDSLALMMTDAQKKYYNAMKKMGSKKPMKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTKVLDSLNMIFIVIFSTECLMKIFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCKPPNSEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDLFDVLEPPLQIHKPNKFKIYSMDIPICKGDMMFCVDILDALTKDFFARKGNEIEETAELSEVQPRQNEPGYEQVSSTLYRQREEYCARLIQNAWRKYRQRTGGAPEQVNVDGAAGEGDGEVEVHQTAVLVESDGFVTKNGHKVVLHSRTPSISSKSADV

Target ORF information:

RefSeq Version	XM_015982131.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic B (Para), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015982131.1

ORF Insert Sequence:

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGCCAATAAT GACGACGAAG AGGAAGAGCC GACGTTCAGG 
GAACGCTTCC TGGCTTATGC CATCAAAATC ATTGACATGT TTTGCGTATG GGATTGTTGT 
AAATGTTGGG TCGTTATTCA GAAGTTTGTC GCCCTTCTTG TCTTCGACCC ATTCGTCGAG 
CTTTTTATCA CGCTGTGCAT TGTTGTTAAC ACCTTGTTTA TGGCATTGGA TCACCACGAC 
ATGGACAAGG ATTTGGAAAA GGCTTTGAAG AGCGGAAACT ACTTTTTTAC TGCGACTTTT 
ATGATTGAAG CTACTTTAAA ATTGATTGCA ATGAGTCCGA AATACTACTT TCAAGAAGGC 
TGGAACATTT TTGATTTTAT TATAGTGGCG TTATCGTTGC TGGAGCTAGG ACTGGAAGGC 
GTTCAAGGCT TATCGGTCTT GCGTTCATTT CGACTGTTAC GAGTGTTCAA ACTGGCCAAG 
TCGTGGCCAA CTTTAAATTT ACTCATTTCC ATAATGGGTA GAACGATGGG TGCTTTAGGA 
AACTTGACTT TTGTGCTCTG TATTATAATA TTTATTTTTG CCGTTATGGG CATGCAACTG 
TTTGGGAAAA ATTACACAGA TAACGTGGAT CGGTTTCCCG ACCACGAGCT CCCCCGCTGG 
AACTTCACCG ACTTCATGCA TTCGTTTATG ATAGTTTTTC GCGTTTTGTG TGGCGAATGG 
ATCGAGTCCA TGTGGGACTG TATGCTGGTG GGCGACGTCT CTTGCATTCC GTTCTTTCTC 
GCAACTGTCG TGATTGGCAA TCTTGTGGTC CTTAATCTTT TCTTAGCCCT GCTTTTGAGT 
AACTTTGGAT CTTCGAGTCT GTCGGCGCCG ACTGCCGACA ATGACACGAA TAAAATAGCA 
GAGGCTTTTG ACAGAATCGG CCGCTTTATC AGATGGATCA AAGGCAATGT TTTACATATC 
GCAAAACTGG TCCGATTCAA GCTCACCAAC CAAATTTCCG ACCAGCCGTC AGGTGAGGGA 
CCGTCCAACA GTTGGAACCA AGATGTGAGA TGTGATGGTG GTTTAGATAT CTCCGGAGAC 
GAAATTTTAG CCGACGGTAT AATTAAAAAA AGTCCTAAAG ACCGACTGGA AGTAACAATT 
GGCACCGGAA TGGATCTTAC CGTGCATGGT GATCCCAAAA GAGGCAAAAA CAATATTAAC 
AAAAGCAAAA CAATCGGAAA TTCAATTCTG GAACATGGAA TGTACATCGG TCACCTGGAC 
GAAGACGAAA TTAGTAACAA ATCGTACGGT AGTCACAAAA ATCGATTTAA AGAGGAAAGT 
CATAAAGGGA GTGTTGATAT GTTAGACGAC CATGAGGAGA AACGAGATGC TAGTAAAGAA 
GAATTAGGAA TTGGTGAAGA AGAAACAGAA GACGACAGAT TTGACTTGGA AAAACCACTG 
ACTGACATTG TGATTGTGGG AAACGCTGAC GATATAATTA TCGACGATTA CGCAGCCGAC 
TGCTTTCCCG ATTCATGTTA CAAGAAGTTT CCGTTTCTTG CAGGCGACGA GGATTCGCCG 
TTTTGGCAGG GTTGGGGGAA TTTAAGGTCG AAGACTTATA GACTAATAGA GAACAAATAT 
TTTGAAACGG CCGTAATCAC GATGATTTTG TTGAGTAGCA TGGCGTTGGC GTTAGAAGAC 
GTACATTTGC AAAGTCGGCC GATTTTGCAA GACATACTGT ATTACATGGA TCGAATATTC 
ACCGTGATAT TCTTTTTCGA GATGTTGATA AAGTGGTTAG CGTTAGGGTT CCAAAAATAC 
TTTACGAACG CTTGGTGCTG GCTAGATTTT GTGATTGTAA TGGTGTCTCT CATTAATTTT 
ATTGCCTCTC TTTGTGGCGC TGGCGGGATT CAAGCATTTA AAACTATGCG AACGCTGCGG 
GCTTTGAGAC CACTTCGCGC CATGTCCCGC ATGCAAGGGA TGAGGGTTGT CGTTAATGCA 
TTGGTGCAAG CGATACCGTC TATCTTCAAT GTACTGCTAG TGTGCTTAAT ATTTTGGTTA 
ATTTTTGCTA TAATGGGTGT GCAGTTATTC GCTGGTAAAT ACTTTAAGTG CGTGGATGCA 
AACAAAACCA CACTAAGCTA TGAGATTATC CCTGATGTTA ACGCGTGTAA AGCTGAGAAT 
TACACGTGGG AAAACTCCCC AATGAATTTT GATCATGTGG GCAAAGCATA TTTGTGCTTA 
TTTCAAGTGG CGACATTCAA GGGGTGGATT CAAATTATGA ACGATGCTAT AGATTCTAGA 
GACATCAACA AACAACCAAT AAGAGAAACA AACATTTATA TGTATTTATA CTTCGTATTT 
TTTATAATAT TTGGATCGTT TTTTACACTT AACTTATTCA TTGGTGTAAT CATCGATAAT 
TTTAACGAAC AAAAGAAAAA GGGTGGCGAT TCATTGGCAC TGATGATGAC AGACGCCCAG 
AAGAAATATT ACAATGCGAT GAAAAAAATG GGCTCTAAGA AACCAATGAA GGCGATACCG 
AGGCCGAGGT GGAGACCTCA AGCTATAGTT TTCGAAATAG TAACAAATAA GAAATTTGAT 
ATGATAATCA TGTTGTTTAT TGGACTGAAC ATGCTTACGA TGACAATGGA TCACTACCAA 
CAGAAAGAAA CTTTCACGAA AGTCCTCGAT TCCCTCAACA TGATTTTCAT TGTTATCTTT 
AGTACCGAGT GCCTCATGAA AATTTTTGCG TTACGTTACC ACTACTTTAC GGAACCTTGG 
AACCTGTTCG ATTTAGTGGT AGTAATATTA TCAATTTTGG GTTTGGTTTT GAGTGACATC 
ATAGAGAAAT ATTTCGTGTC ACCGACGTTG TTAAGGGTGG TTCGTGTGGC CAAAGTAGGG 
CGAGTTCTGC GTTTGGTGAA AGGTGCAAAG GGCATTCGGA CACTGCTGTT CGCACTAGCC 
ATGTCACTGC CGGCACTGTT CAACATCTGT TTGTTGCTGT TCCTGGTCAT GTTCATTTTT 
GCCATCTTCG GGATGTCGTT CTTCATGCAC GTGAAGGACA AGAGCGGACT TGACGATGTC 
TACAACTTTA AAACGTTTGG CCAGTCGATG ATTCTCCTCT TCCAGATGTC CACTTCGGCG 
GGCTGGGATG GAGTCCTGGA TGGTATTATC AATGAAGAAG ATTGTAAACC GCCAAACAGT 
GAGATCGGCG AGACCGGAAA TTGCGGGAAT TCGACAATTG GTATCGCATT CCTGCTCAGC 
TATCTCGTGA TTTCCTTCCT CATCGTCATC AACATGTACA TCGCTGTGAT TTTGGAGAAC 
TACTCCCAGG CGACGGAGGA TGTGCAAGAG GGTCTGACCG ATGATGACTA CGACATGTAC 
TACGAGATCT GGCAGCAGTT CGACCCTGAC GGCACCCAGT ACATCCGCTA CGACCAACTG 
TCGGACTTGT TCGATGTGTT GGAGCCTCCG CTACAGATCC ACAAGCCCAA CAAGTTCAAA 
ATCTACTCCA TGGACATTCC GATCTGCAAG GGTGACATGA TGTTTTGTGT GGACATTTTG 
GACGCGCTAA CAAAAGACTT CTTCGCTCGC AAGGGCAACG AAATCGAGGA AACTGCTGAA 
CTTAGTGAAG TGCAACCGAG ACAGAACGAA CCGGGTTACG AACAAGTCAG TTCGACGCTT 
TACAGACAGC GCGAAGAATA TTGTGCGAGA CTGATCCAGA ATGCCTGGCG CAAGTATAGA 
CAGCGCACTG GCGGTGCTCC AGAGCAAGTG AATGTCGATG GGGCTGCCGG CGAGGGCGAT 
GGGGAGGTGG AGGTCCACCA AACGGCCGTT CTGGTCGAGA GCGACGGCTT TGTGACCAAG 
AACGGGCACA AGGTTGTGCT GCACAGTCGC ACACCCAGCA TCAGCTCCAA GTCTGCGGAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015982158.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837644.1 Tribolium castaneum paralytic B (Para), transcript variant X2, mRNA.

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CloneID	OTg16172
Clone ID Related Accession (Same CDS sequence)	XM_015982158.1
Accession Version	XM_015982158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGTTTCTGTT TATTATTTCT CAGCCAATAA TGACGACGAA 
GAGGAAGAGC CGACGTTCAG GGAACGCTTC CTGGCTTATG CCATCAAAAT CATTGACATG 
TTTTGCGTAT GGGATTGTTG TAAATGTTGG GTCGTTATTC AGAAGTTTGT CGCCCTTCTT 
GTCTTCGACC CATTCGTCGA GCTTTTTATC ACGCTGTGCA TTGTTGTTAA CACCTTGTTT 
ATGGCATTGG ATCACCACGA CATGGACAAG GATTTGGAAA AGGCTTTGAA GAGCGGAAAC 
TACTTTTTTA CTGCGACTTT TATGATTGAA GCTACTTTAA AATTGATTGC AATGAGTCCG 
AAATACTACT TTCAAGAAGG CTGGAACATT TTTGATTTTA TTATAGTGGC GTTATCGTTG 
CTGGAGCTAG GACTGGAAGG CGTTCAAGGC TTATCGGTCT TGCGTTCATT TCGACTGTTA 
CGAGTGTTCA AACTGGCCAA GTCGTGGCCA ACTTTAAATT TACTCATTTC CATAATGGGT 
AGAACGATGG GTGCTTTAGG AAACTTGACT TTTGTGCTCT GTATTATAAT ATTTATTTTT 
GCCGTTATGG GCATGCAACT GTTTGGGAAA AATTACACAG ATAACGTGGA TCGGTTTCCC 
GACCACGAGC TCCCCCGCTG GAACTTCACC GACTTCATGC ATTCGTTTAT GATAGTTTTT 
CGCGTTTTGT GTGGCGAATG GATCGAGTCC ATGTGGGACT GTATGCTGGT GGGCGACGTC 
TCTTGCATTC CGTTCTTTCT CGCAACTGTC GTGATTGGCA ATCTTGTGGT CCTTAATCTT 
TTCTTAGCCC TGCTTTTGAG TAACTTTGGA TCTTCGAGTC TGTCGGCGCC GACTGCCGAC 
AATGACACGA ATAAAATAGC AGAGGCTTTT GACAGAATCG GCCGCTTTAT CAGATGGATC 
AAAGGCAATG TTTTACATAT CGCAAAACTG GTCCGATTCA AGCTCACCAA CCAAATTTCC 
GACCAGCCGT CAGATGTGAG ATGTGATGGT GGTTTAGATA TCTCCGGAGA CGAAATTTTA 
GCCGACGGTA TAATTAAAAA AAGTCCTAAA GACCGACTGG AAGTAACAAT TGGCACCGGA 
ATGGATCTTA CCGTGCATGG TGATCCCAAA AGAGGCAAAA ACAATATTAA CAAAAGCAAA 
ACAATCGGAA ATTCAATTCT GGAACATGGA ATGTACATCG GTCACCTGGA CGAAGACGAA 
ATTAGTAACA AATCGTACGG TAGTCACAAA AATCGATTTA AAGAGGAAAG TCATAAAGGG 
AGTGTTGATA TGTTAGACGA CCATGAGGAG AAACGAGATG CTAGTAAAGA AGAATTAGGA 
ATTGGTGAAG AAGAAACAGA AGACGACAGA TTTGACTTGG AAAAACCACT GACTGACATT 
GTGATTGTGG GAAACGCTGA CGATATAATT ATCGACGATT ACGCAGCCGA CTGCTTTCCC 
GATTCATGTT ACAAGAAGTT TCCGTTTCTT GCAGGCGACG AGGATTCGCC GTTTTGGCAG 
GGTTGGGGGA ATTTAAGGTC GAAGACTTAT AGACTAATAG AGAACAAATA TTTTGAAACG 
GCCGTAATCA CGATGATTTT GTTGAGTAGC ATGGCGTTGG CGTTAGAAGA CGTACATTTG 
CAAAGTCGGC CGATTTTGCA AGACATACTG TATTACATGG ATCGAATATT CACCGTGATA 
TTCTTTTTCG AGATGTTGAT AAAGTGGTTA GCGTTAGGGT TCCAAAAATA CTTTACGAAC 
GCTTGGTGCT GGCTAGATTT TGTGATTGTA ATGGTGTCTC TCATTAATTT TATTGCCTCT 
CTTTGTGGCG CTGGCGGGAT TCAAGCATTT AAAACTATGC GAACGCTGCG GGCTTTGAGA 
CCACTTCGCG CCATGTCCCG CATGCAAGGG ATGAGGGTTG TCGTTAATGC ATTGGTGCAA 
GCGATACCGT CTATCTTCAA TGTACTGCTA GTGTGCTTAA TATTTTGGTT AATTTTTGCT 
ATAATGGGTG TGCAGTTATT CGCTGGTAAA TACTTTAAGT GCGTGGATGC AAACAAAACC 
ACACTAAGCT ATGAGATTAT CCCTGATGTT AACGCGTGTA AAGCTGAGAA TTACACGTGG 
GAAAACTCCC CAATGAATTT TGATCATGTG GGCAAAGCAT ATTTGTGCTT ATTTCAAGTG 
GCGACATTCA AGGGGTGGAT TCAAATTATG AACGATGCTA TAGATTCTAG AGACATCAAC 
AAACAACCAA TAAGAGAAAC AAACATTTAT ATGTATTTAT ACTTCGTATT TTTTATAATA 
TTTGGATCGT TTTTTACACT TAACTTATTC ATTGGTGTAA TCATCGATAA TTTTAACGAA 
CAAAAGAAAA AGGGTGGCGA TTCATTGGCA CTGATGATGA CAGACGCCCA GAAGAAATAT 
TACAATGCGA TGAAAAAAAT GGGCTCTAAG AAACCAATGA AGGCGATACC GAGGCCGAGG 
TGGAGACCTC AAGCTATAGT TTTCGAAATA GTAACAAATA AGAAATTTGA TATGATAATC 
ATGTTGTTTA TTGGACTGAA CATGCTTACG ATGACAATGG ATCACTACCA ACAGAAAGAA 
ACTTTCACGA AAGTCCTCGA TTCCCTCAAC ATGATTTTCA TTGTTATCTT TAGTACCGAG 
TGCCTCATGA AAATTTTTGC GTTACGTTAC CACTACTTTA CGGAACCTTG GAACCTGTTC 
GATTTAGTGG TAGTAATATT ATCAATTTTG GGTTTGGTTT TGAGTGACAT CATAGAGAAA 
TATTTCGTGT CACCGACGTT GTTAAGGGTG GTTCGTGTGG CCAAAGTAGG GCGAGTTCTG 
CGTTTGGTGA AAGGTGCAAA GGGCATTCGG ACACTGCTGT TCGCACTAGC CATGTCACTG 
CCGGCACTGT TCAACATCTG TTTGTTGCTG TTCCTGGTCA TGTTCATTTT TGCCATCTTC 
GGGATGTCGT TCTTCATGCA CGTGAAGGAC AAGAGCGGAC TTGACGATGT CTACAACTTT 
AAAACGTTTG GCCAGTCGAT GATTCTCCTC TTCCAGATGT CCACTTCGGC GGGCTGGGAT 
GGAGTCCTGG ATGGTATTAT CAATGAAGAA GATTGTAAAC CGCCAAACAG TGAGATCGGC 
GAGACCGGAA ATTGCGGGAA TTCGACAATT GGTATCGCAT TCCTGCTCAG CTATCTCGTG 
ATTTCCTTCC TCATCGTCAT CAACATGTAC ATCGCTGTGA TTTTGGAGAA CTACTCCCAG 
GCGACGGAGG ATGTGCAAGA GGGTCTGACC GATGATGACT ACGACATGTA CTACGAGATC 
TGGCAGCAGT TCGACCCTGA CGGCACCCAG TACATCCGCT ACGACCAACT GTCGGACTTG 
TTCGATGTGT TGGAGCCTCC GCTACAGATC CACAAGCCCA ACAAGTTCAA AATCTACTCC 
ATGGACATTC CGATCTGCAA GGGTGACATG ATGTTTTGTG TGGACATTTT GGACGCGCTA 
ACAAAAGACT TCTTCGCTCG CAAGGGCAAC GAAATCGAGG AAACTGCTGA ACTTAGTGAA 
GTGCAACCGA GACAGAACGA ACCGGGTTAC GAACAAGTCA GTTCGACGCT TTACAGACAG 
CGCGAAGAAT ATTGTGCGAG ACTGATCCAG AATGCCTGGC GCAAGTATAG ACAGCGCACT 
GGCGGTGCTC CAGAGCAAGT GAATGTCGAT GGGGCTGCCG GCGAGGGCGA TGGGGAGGTG 
GAGGTCCACC AAACGGCCGT TCTGGTCGAG AGCGACGGCT TTGTGACCAA GAACGGGCAC 
AAGGTTGTGC TGCACAGTCG CACACCCAGC ATCAGCTCCA AGTCTGCGGA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837644.1
CDS	589..6705
Translation	MSVASDYSSEEELSLFRPFTRESLAVIETRIAEEYAKQKELEKKRAEGETGFGRRKKKKDIRYEDEDEDEGPQPDPTLEQGLPLPVRLHSTFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALIGLQIYMGVLTQKCIKEFPLDGSWGNLTHENWERFVSNESNWYWDDEKGERPLCGNSSGSGMCKEGYVCLQGYGDNPNYGYTSFDTFGWALLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEANAIREAEQKAKDKQDRADARAAAAVEAAEAAAAAAAAAAAGCDNDQYIIKSSSDYSCHSYESFVGQPNGQDDNNKEKISIRSEGLDSVSEQRRVPTNLSKMRKVSAASLSLPGSPFNLRRGSRGSHQFTLRNNRRMVAPPGDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGVMVLPMYYTNLGSRHSSYTSHASRMSYTSHGDLLGGLGSNGKMMTKESQLRNRSLRNGPAAAPLNHKSTRGVYDGPTGHICESKVKQIDNPFIDSNQRQTVVDMKDVMVLNDIIEQAAVSGGSDRGVSVYYFSANNDDEEEEPTFRERFLAYAIKIIDMFCVWDCCKCWVVIQKFVALLVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATLKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFDRIGRFIRWIKGNVLHIAKLVRFKLTNQISDQPSDVRCDGGLDISGDEILADGIIKKSPKDRLEVTIGTGMDLTVHGDPKRGKNNINKSKTIGNSILEHGMYIGHLDEDEISNKSYGSHKNRFKEESHKGSVDMLDDHEEKRDASKEELGIGEEETEDDRFDLEKPLTDIVIVGNADDIIIDDYAADCFPDSCYKKFPFLAGDEDSPFWQGWGNLRSKTYRLIENKYFETAVITMILLSSMALALEDVHLQSRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFVIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTTLSYEIIPDVNACKAENYTWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKGGDSLALMMTDAQKKYYNAMKKMGSKKPMKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTKVLDSLNMIFIVIFSTECLMKIFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCKPPNSEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDLFDVLEPPLQIHKPNKFKIYSMDIPICKGDMMFCVDILDALTKDFFARKGNEIEETAELSEVQPRQNEPGYEQVSSTLYRQREEYCARLIQNAWRKYRQRTGGAPEQVNVDGAAGEGDGEVEVHQTAVLVESDGFVTKNGHKVVLHSRTPSISSKSADV

Target ORF information:

RefSeq Version	XM_015982158.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic B (Para), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015982158.1

ORF Insert Sequence:

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GAAGGAAAAA GAAAAAAGAT 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGTCCCCAGC CTGATCCAAC TCTGGAGCAG 
GGGCTGCCCT TGCCAGTACG TCTTCACAGT ACCTTTCCTC CGGAACTGGC CAGTACACCA 
CTTGAAGACA TCGACCCTTT TTACAGTAAC CAAAGGACTT TTGTAGTGGT TAGTAAAGGC 
AAGGACATTT TCCGGTTTAG CGCCACAAAC GCCCTGTGGG TACTCGATCC CTTCAATCCG 
ATCCGTCGAG TGGCCATTTA CATTCTAGTG CATCCTTTGT TTTCTCTTTT TATCATTACC 
ACAATCCTCG TTAACTGTAT CCTTATGATC ATGCCCACAA CACCCACCGT AGAGTCAACC 
GAAGTGATAT TCACAGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCGAGAGGT 
TTTATCCTAC AACCGTTCAC CTACCTTAGA GATGCATGGA ACTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTCAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC AATGTTTTCC 
CTGTCAGTGT TCGCATTGAT AGGTTTACAA ATTTATATGG GTGTTTTAAC ACAAAAATGT 
ATCAAAGAAT TTCCTCTGGA TGGCTCTTGG GGGAACCTCA CCCACGAAAA CTGGGAGAGA 
TTTGTTAGCA ACGAATCCAA CTGGTATTGG GATGACGAAA AAGGCGAAAG GCCTCTGTGT 
GGAAACTCCT CAGGATCGGG AATGTGCAAA GAGGGTTACG TTTGTTTGCA AGGTTACGGT 
GATAACCCAA ACTATGGTTA CACCAGCTTT GATACTTTTG GTTGGGCATT ACTTTCCGCC 
TTCAGATTAA TGACTCAGGA TTATTGGGAG AATTTATACC AACTGGTTTT AAGATCAGCC 
GGTCCTTGGC ATATGCTATT TTTTATCGTG ATTATATTTT TGGGTTCGTT TTATTTAGTT 
AACTTGATCT TGGCTATCGT CGCTATGTCG TACGACGAGT TGCAGAAGAA AGCCCAAGAT 
GAGGAAGAAG CTGAAGCTAA CGCTATAAGG GAAGCCGAGC AAAAGGCAAA GGACAAGCAA 
GATCGGGCGG ATGCGAGGGC AGCGGCGGCC GTGGAGGCGG CCGAGGCAGC GGCCGCTGCC 
GCGGCGGCGG CGGCAGCAGG GTGTGACAAC GACCAGTACA TCATAAAATC GTCTTCGGAC 
TACTCGTGTC ACAGCTATGA GTCGTTCGTG GGGCAACCCA ACGGTCAAGA CGACAACAAC 
AAGGAGAAGA TTAGCATACG CTCGGAAGGA TTAGATTCCG TGAGTGAACA GAGAAGAGTT 
CCTACTAATC TGAGCAAGAT GCGGAAAGTC AGCGCTGCCA GTCTCAGCCT CCCCGGCTCT 
CCCTTCAACC TTCGGCGAGG GTCCCGAGGT AGCCACCAGT TCACCTTGCG GAACAATAGG 
CGCATGGTGG CTCCGCCGGG CGATCGAAAG CCCTTGGTGC TGTCGACCTA CCTTGACGCC 
CAAGAGCACC TCCCCTACGC CGACGACTCT AACGCCGTCA CCCCCATGAG CGAGGAGAAC 
GGCGTCATGG TCCTGCCCAT GTACTACACC AACCTGGGAT CACGTCATTC ATCGTACACT 
TCACATGCCT CACGGATGTC ATACACTTCT CATGGAGATT TGTTAGGCGG TTTGGGAAGC 
AATGGCAAAA TGATGACGAA GGAGAGCCAG CTCAGGAACC GATCGCTGAG AAACGGACCA 
GCGGCCGCTC CCCTAAACCA CAAGTCAACG CGAGGCGTCT ACGATGGTCC CACGGGGCAC 
ATTTGCGAAT CAAAAGTTAA GCAAATTGAC AATCCTTTTA TCGATAGCAA TCAGCGACAG 
ACAGTCGTGG ACATGAAAGA TGTTATGGTG CTGAACGACA TCATCGAGCA AGCTGCCGTG 
AGCGGAGGCA GTGACCGCGG AGTTTCTGTT TATTATTTCT CAGCCAATAA TGACGACGAA 
GAGGAAGAGC CGACGTTCAG GGAACGCTTC CTGGCTTATG CCATCAAAAT CATTGACATG 
TTTTGCGTAT GGGATTGTTG TAAATGTTGG GTCGTTATTC AGAAGTTTGT CGCCCTTCTT 
GTCTTCGACC CATTCGTCGA GCTTTTTATC ACGCTGTGCA TTGTTGTTAA CACCTTGTTT 
ATGGCATTGG ATCACCACGA CATGGACAAG GATTTGGAAA AGGCTTTGAA GAGCGGAAAC 
TACTTTTTTA CTGCGACTTT TATGATTGAA GCTACTTTAA AATTGATTGC AATGAGTCCG 
AAATACTACT TTCAAGAAGG CTGGAACATT TTTGATTTTA TTATAGTGGC GTTATCGTTG 
CTGGAGCTAG GACTGGAAGG CGTTCAAGGC TTATCGGTCT TGCGTTCATT TCGACTGTTA 
CGAGTGTTCA AACTGGCCAA GTCGTGGCCA ACTTTAAATT TACTCATTTC CATAATGGGT 
AGAACGATGG GTGCTTTAGG AAACTTGACT TTTGTGCTCT GTATTATAAT ATTTATTTTT 
GCCGTTATGG GCATGCAACT GTTTGGGAAA AATTACACAG ATAACGTGGA TCGGTTTCCC 
GACCACGAGC TCCCCCGCTG GAACTTCACC GACTTCATGC ATTCGTTTAT GATAGTTTTT 
CGCGTTTTGT GTGGCGAATG GATCGAGTCC ATGTGGGACT GTATGCTGGT GGGCGACGTC 
TCTTGCATTC CGTTCTTTCT CGCAACTGTC GTGATTGGCA ATCTTGTGGT CCTTAATCTT 
TTCTTAGCCC TGCTTTTGAG TAACTTTGGA TCTTCGAGTC TGTCGGCGCC GACTGCCGAC 
AATGACACGA ATAAAATAGC AGAGGCTTTT GACAGAATCG GCCGCTTTAT CAGATGGATC 
AAAGGCAATG TTTTACATAT CGCAAAACTG GTCCGATTCA AGCTCACCAA CCAAATTTCC 
GACCAGCCGT CAGATGTGAG ATGTGATGGT GGTTTAGATA TCTCCGGAGA CGAAATTTTA 
GCCGACGGTA TAATTAAAAA AAGTCCTAAA GACCGACTGG AAGTAACAAT TGGCACCGGA 
ATGGATCTTA CCGTGCATGG TGATCCCAAA AGAGGCAAAA ACAATATTAA CAAAAGCAAA 
ACAATCGGAA ATTCAATTCT GGAACATGGA ATGTACATCG GTCACCTGGA CGAAGACGAA 
ATTAGTAACA AATCGTACGG TAGTCACAAA AATCGATTTA AAGAGGAAAG TCATAAAGGG 
AGTGTTGATA TGTTAGACGA CCATGAGGAG AAACGAGATG CTAGTAAAGA AGAATTAGGA 
ATTGGTGAAG AAGAAACAGA AGACGACAGA TTTGACTTGG AAAAACCACT GACTGACATT 
GTGATTGTGG GAAACGCTGA CGATATAATT ATCGACGATT ACGCAGCCGA CTGCTTTCCC 
GATTCATGTT ACAAGAAGTT TCCGTTTCTT GCAGGCGACG AGGATTCGCC GTTTTGGCAG 
GGTTGGGGGA ATTTAAGGTC GAAGACTTAT AGACTAATAG AGAACAAATA TTTTGAAACG 
GCCGTAATCA CGATGATTTT GTTGAGTAGC ATGGCGTTGG CGTTAGAAGA CGTACATTTG 
CAAAGTCGGC CGATTTTGCA AGACATACTG TATTACATGG ATCGAATATT CACCGTGATA 
TTCTTTTTCG AGATGTTGAT AAAGTGGTTA GCGTTAGGGT TCCAAAAATA CTTTACGAAC 
GCTTGGTGCT GGCTAGATTT TGTGATTGTA ATGGTGTCTC TCATTAATTT TATTGCCTCT 
CTTTGTGGCG CTGGCGGGAT TCAAGCATTT AAAACTATGC GAACGCTGCG GGCTTTGAGA 
CCACTTCGCG CCATGTCCCG CATGCAAGGG ATGAGGGTTG TCGTTAATGC ATTGGTGCAA 
GCGATACCGT CTATCTTCAA TGTACTGCTA GTGTGCTTAA TATTTTGGTT AATTTTTGCT 
ATAATGGGTG TGCAGTTATT CGCTGGTAAA TACTTTAAGT GCGTGGATGC AAACAAAACC 
ACACTAAGCT ATGAGATTAT CCCTGATGTT AACGCGTGTA AAGCTGAGAA TTACACGTGG 
GAAAACTCCC CAATGAATTT TGATCATGTG GGCAAAGCAT ATTTGTGCTT ATTTCAAGTG 
GCGACATTCA AGGGGTGGAT TCAAATTATG AACGATGCTA TAGATTCTAG AGACATCAAC 
AAACAACCAA TAAGAGAAAC AAACATTTAT ATGTATTTAT ACTTCGTATT TTTTATAATA 
TTTGGATCGT TTTTTACACT TAACTTATTC ATTGGTGTAA TCATCGATAA TTTTAACGAA 
CAAAAGAAAA AGGGTGGCGA TTCATTGGCA CTGATGATGA CAGACGCCCA GAAGAAATAT 
TACAATGCGA TGAAAAAAAT GGGCTCTAAG AAACCAATGA AGGCGATACC GAGGCCGAGG 
TGGAGACCTC AAGCTATAGT TTTCGAAATA GTAACAAATA AGAAATTTGA TATGATAATC 
ATGTTGTTTA TTGGACTGAA CATGCTTACG ATGACAATGG ATCACTACCA ACAGAAAGAA 
ACTTTCACGA AAGTCCTCGA TTCCCTCAAC ATGATTTTCA TTGTTATCTT TAGTACCGAG 
TGCCTCATGA AAATTTTTGC GTTACGTTAC CACTACTTTA CGGAACCTTG GAACCTGTTC 
GATTTAGTGG TAGTAATATT ATCAATTTTG GGTTTGGTTT TGAGTGACAT CATAGAGAAA 
TATTTCGTGT CACCGACGTT GTTAAGGGTG GTTCGTGTGG CCAAAGTAGG GCGAGTTCTG 
CGTTTGGTGA AAGGTGCAAA GGGCATTCGG ACACTGCTGT TCGCACTAGC CATGTCACTG 
CCGGCACTGT TCAACATCTG TTTGTTGCTG TTCCTGGTCA TGTTCATTTT TGCCATCTTC 
GGGATGTCGT TCTTCATGCA CGTGAAGGAC AAGAGCGGAC TTGACGATGT CTACAACTTT 
AAAACGTTTG GCCAGTCGAT GATTCTCCTC TTCCAGATGT CCACTTCGGC GGGCTGGGAT 
GGAGTCCTGG ATGGTATTAT CAATGAAGAA GATTGTAAAC CGCCAAACAG TGAGATCGGC 
GAGACCGGAA ATTGCGGGAA TTCGACAATT GGTATCGCAT TCCTGCTCAG CTATCTCGTG 
ATTTCCTTCC TCATCGTCAT CAACATGTAC ATCGCTGTGA TTTTGGAGAA CTACTCCCAG 
GCGACGGAGG ATGTGCAAGA GGGTCTGACC GATGATGACT ACGACATGTA CTACGAGATC 
TGGCAGCAGT TCGACCCTGA CGGCACCCAG TACATCCGCT ACGACCAACT GTCGGACTTG 
TTCGATGTGT TGGAGCCTCC GCTACAGATC CACAAGCCCA ACAAGTTCAA AATCTACTCC 
ATGGACATTC CGATCTGCAA GGGTGACATG ATGTTTTGTG TGGACATTTT GGACGCGCTA 
ACAAAAGACT TCTTCGCTCG CAAGGGCAAC GAAATCGAGG AAACTGCTGA ACTTAGTGAA 
GTGCAACCGA GACAGAACGA ACCGGGTTAC GAACAAGTCA GTTCGACGCT TTACAGACAG 
CGCGAAGAAT ATTGTGCGAG ACTGATCCAG AATGCCTGGC GCAAGTATAG ACAGCGCACT 
GGCGGTGCTC CAGAGCAAGT GAATGTCGAT GGGGCTGCCG GCGAGGGCGA TGGGGAGGTG 
GAGGTCCACC AAACGGCCGT TCTGGTCGAG AGCGACGGCT TTGTGACCAA GAACGGGCAC 
AAGGTTGTGC TGCACAGTCG CACACCCAGC ATCAGCTCCA AGTCTGCGGA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015982185.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837671.1 Tribolium castaneum paralytic B (Para), transcript variant X3, mRNA.

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CloneID	OTg16173
Clone ID Related Accession (Same CDS sequence)	XM_015982185.1
Accession Version	XM_015982185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGT 
CCCCAGCCTG ATCCAACTCT GGAGCAGGGG CTGCCCTTGC CAGTACGTCT TCACAGTACC 
TTTCCTCCGG AACTGGCCAG TACACCACTT GAAGACATCG ACCCTTTTTA CAGTAACCAA 
AGGACTTTTG TAGTGGTTAG TAAAGGCAAG GACATTTTCC GGTTTAGCGC CACAAACGCC 
CTGTGGGTAC TCGATCCCTT CAATCCGATC CGTCGAGTGG CCATTTACAT TCTAGTGCAT 
CCTTTGTTTT CTCTTTTTAT CATTACCACA ATCCTCGTTA ACTGTATCCT TATGATCATG 
CCCACAACAC CCACCGTAGA GTCAACCGAA GTGATATTCA CAGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC GAGAGGTTTT ATCCTACAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAACT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTCACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACAAT GTTTTCCCTG TCAGTGTTCG CATTGATAGG TTTACAAATT 
TATATGGGTG TTTTAACACA AAAATGTATC AAAGAATTTC CTCTGGATGG CTCTTGGGGG 
AACCTCACCC ACGAAAACTG GGAGAGATTT GTTAGCAACG AATCCAACTG GTATTGGGAT 
GACGAAAAAG GCGAAAGGCC TCTGTGTGGA AACTCCTCAG GATCGGGAAT GTGCAAAGAG 
GGTTACGTTT GTTTGCAAGG TTACGGTGAT AACCCAAACT ATGGTTACAC CAGCTTTGAT 
ACTTTTGGTT GGGCATTACT TTCCGCCTTC AGATTAATGA CTCAGGATTA TTGGGAGAAT 
TTATACCAAC TGGTTTTAAG ATCAGCCGGT CCTTGGCATA TGCTATTTTT TATCGTGATT 
ATATTTTTGG GTTCGTTTTA TTTAGTTAAC TTGATCTTGG CTATCGTCGC TATGTCGTAC 
GACGAGTTGC AGAAGAAAGC CCAAGATGAG GAAGAAGCTG AAGCTAACGC TATAAGGGAA 
GCCGAGCAAA AGGCAAAGGA CAAGCAAGAT CGGGCGGATG CGAGGGCAGC GGCGGCCGTG 
GAGGCGGCCG AGGCAGCGGC CGCTGCCGCG GCGGCGGCGG CAGCAGGGTG TGACAACGAC 
CAGTACATCA TAAAATCGTC TTCGGACTAC TCGTGTCACA GCTATGAGTC GTTCGTGGGG 
CAACCCAACG GTCAAGACGA CAACAACAAG GAGAAGATTA GCATACGCTC GGAAGGATTA 
GATTCCGTGA GTGAACAGAG AAGAGTTCCT ACTAATCTGA GCAAGATGCG GAAAGTCAGC 
GCTGCCAGTC TCAGCCTCCC CGGCTCTCCC TTCAACCTTC GGCGAGGGTC CCGAGGTAGC 
CACCAGTTCA CCTTGCGGAA CAATAGGCGC ATGGTGGCTC CGCCGGGCGA TCGAAAGCCC 
TTGGTGCTGT CGACCTACCT TGACGCCCAA GAGCACCTCC CCTACGCCGA CGACTCTAAC 
GCCGTCACCC CCATGAGCGA GGAGAACGGC GTCATGGTCC TGCCCATGTA CTACACCAAC 
CTGGGATCAC GTCATTCATC GTACACTTCA CATGCCTCAC GGATGTCATA CACTTCTCAT 
GGAGATTTGT TAGGCGGTTT GGGAAGCAAT GGCAAAATGA TGACGAAGGA GAGCCAGCTC 
AGGAACCGAT CGCTGAGAAA CGGACCAGCG GCCGCTCCCC TAAACCACAA GTCAACGCGA 
GGCGTCTACG ATGGTCCCAC GGGGCACATT TGCGAATCAA AAGTTAAGCA AATTGACAAT 
CCTTTTATCG ATAGCAATCA GCGACAGACA GTCGTGGACA TGAAAGATGT TATGGTGCTG 
AACGACATCA TCGAGCAAGC TGCCGTGAGC GGAGGCAGTG ACCGCGGAGT TTCTGTTTAT 
TATTTCTCAG CCAATAATGA CGACGAAGAG GAAGAGCCGA CGTTCAGGGA ACGCTTCCTG 
GCTTATGCCA TCAAAATCAT TGACATGTTT TGCGTATGGG ATTGTTGTAA ATGTTGGGTC 
GTTATTCAGA AGTTTGTCGC CCTTCTTGTC TTCGACCCAT TCGTCGAGCT TTTTATCACG 
CTGTGCATTG TTGTTAACAC CTTGTTTATG GCATTGGATC ACCACGACAT GGACAAGGAT 
TTGGAAAAGG CTTTGAAGAG CGGAAACTAC TTTTTTACTG CGACTTTTAT GATTGAAGCT 
ACTTTAAAAT TGATTGCAAT GAGTCCGAAA TACTACTTTC AAGAAGGCTG GAACATTTTT 
GATTTTATTA TAGTGGCGTT ATCGTTGCTG GAGCTAGGAC TGGAAGGCGT TCAAGGCTTA 
TCGGTCTTGC GTTCATTTCG ACTGTTACGA GTGTTCAAAC TGGCCAAGTC GTGGCCAACT 
TTAAATTTAC TCATTTCCAT AATGGGTAGA ACGATGGGTG CTTTAGGAAA CTTGACTTTT 
GTGCTCTGTA TTATAATATT TATTTTTGCC GTTATGGGCA TGCAACTGTT TGGGAAAAAT 
TACACAGATA ACGTGGATCG GTTTCCCGAC CACGAGCTCC CCCGCTGGAA CTTCACCGAC 
TTCATGCATT CGTTTATGAT AGTTTTTCGC GTTTTGTGTG GCGAATGGAT CGAGTCCATG 
TGGGACTGTA TGCTGGTGGG CGACGTCTCT TGCATTCCGT TCTTTCTCGC AACTGTCGTG 
ATTGGCAATC TTGTGGTCCT TAATCTTTTC TTAGCCCTGC TTTTGAGTAA CTTTGGATCT 
TCGAGTCTGT CGGCGCCGAC TGCCGACAAT GACACGAATA AAATAGCAGA GGCTTTTGAC 
AGAATCGGCC GCTTTATCAG ATGGATCAAA GGCAATGTTT TACATATCGC AAAACTGGTC 
CGATTCAAGC TCACCAACCA AATTTCCGAC CAGCCGTCAG GTGAGGGACC GTCCAACAGT 
TGGAACCAAG ATGTGAGATG TGATGGTGGT TTAGATATCT CCGGAGACGA AATTTTAGCC 
GACGGTATAA TTAAAAAAAG TCCTAAAGAC CGACTGGAAG TAACAATTGG CACCGGAATG 
GATCTTACCG TGCATGGTGA TCCCAAAAGA GGCAAAAACA ATATTAACAA AAGCAAAACA 
ATCGGAAATT CAATTCTGGA ACATGGAATG TACATCGGTC ACCTGGACGA AGACGAAATT 
AGTAACAAAT CGTACGGTAG TCACAAAAAT CGATTTAAAG AGGAAAGTCA TAAAGGGAGT 
GTTGATATGT TAGACGACCA TGAGGAGAAA CGAGATGCTA GTAAAGAAGA ATTAGGAATT 
GGTGAAGAAG AAACAGAAGA CGACAGATTT GACTTGGAAA AACCACTGAC TGACATTGTG 
ATTGTGGGAA ACGCTGACGA TATAATTATC GACGATTACG CAGCCGACTG CTTTCCCGAT 
TCATGTTACA AGAAGTTTCC GTTTCTTGCA GGCGACGAGG ATTCGCCGTT TTGGCAGGGT 
TGGGGGAATT TAAGGTCGAA GACTTATAGA CTAATAGAGA ACAAATATTT TGAAACGGCC 
GTAATCACGA TGATTTTGTT GAGTAGCATG GCGTTGGCGT TAGAAGACGT ACATTTGCAA 
AGTCGGCCGA TTTTGCAAGA CATACTGTAT TACATGGATC GAATATTCAC CGTGATATTC 
TTTTTCGAGA TGTTGATAAA GTGGTTAGCG TTAGGGTTCC AAAAATACTT TACGAACGCT 
TGGTGCTGGC TAGATTTTGT GATTGTAATG GTGTCTCTCA TTAATTTTAT TGCCTCTCTT 
TGTGGCGCTG GCGGGATTCA AGCATTTAAA ACTATGCGAA CGCTGCGGGC TTTGAGACCA 
CTTCGCGCCA TGTCCCGCAT GCAAGGGATG AGGGTTGTCG TTAATGCATT GGTGCAAGCG 
ATACCGTCTA TCTTCAATGT ACTGCTAGTG TGCTTAATAT TTTGGTTAAT TTTTGCTATA 
ATGGGTGTGC AGTTATTCGC TGGTAAATAC TTTAAGTGCG TGGATGCAAA CAAAACCACA 
CTAAGCTATG AGATTATCCC TGATGTTAAC GCGTGTAAAG CTGAGAATTA CACGTGGGAA 
AACTCCCCAA TGAATTTTGA TCATGTGGGC AAAGCATATT TGTGCTTATT TCAAGTGGCG 
ACATTCAAGG GGTGGATTCA AATTATGAAC GATGCTATAG ATTCTAGAGA CATCAACAAA 
CAACCAATAA GAGAAACAAA CATTTATATG TATTTATACT TCGTATTTTT TATAATATTT 
GGATCGTTTT TTACACTTAA CTTATTCATT GGTGTAATCA TCGATAATTT TAACGAACAA 
AAGAAAAAGG GTGGCGATTC ATTGGCACTG ATGATGACAG ACGCCCAGAA GAAATATTAC 
AATGCGATGA AAAAAATGGG CTCTAAGAAA CCAATGAAGG CGATACCGAG GCCGAGGTGG 
AGACCTCAAG CTATAGTTTT CGAAATAGTA ACAAATAAGA AATTTGATAT GATAATCATG 
TTGTTTATTG GACTGAACAT GCTTACGATG ACAATGGATC ACTACCAACA GAAAGAAACT 
TTCACGAAAG TCCTCGATTC CCTCAACATG ATTTTCATTG TTATCTTTAG TACCGAGTGC 
CTCATGAAAA TTTTTGCGTT ACGTTACCAC TACTTTACGG AACCTTGGAA CCTGTTCGAT 
TTAGTGGTAG TAATATTATC AATTTTGGGT TTGGTTTTGA GTGACATCAT AGAGAAATAT 
TTCGTGTCAC CGACGTTGTT AAGGGTGGTT CGTGTGGCCA AAGTAGGGCG AGTTCTGCGT 
TTGGTGAAAG GTGCAAAGGG CATTCGGACA CTGCTGTTCG CACTAGCCAT GTCACTGCCG 
GCACTGTTCA ACATCTGTTT GTTGCTGTTC CTGGTCATGT TCATTTTTGC CATCTTCGGG 
ATGTCGTTCT TCATGCACGT GAAGGACAAG AGCGGACTTG ACGATGTCTA CAACTTTAAA 
ACGTTTGGCC AGTCGATGAT TCTCCTCTTC CAGATGTCCA CTTCGGCGGG CTGGGATGGA 
GTCCTGGATG GTATTATCAA TGAAGAAGAT TGTAAACCGC CAAACAGTGA GATCGGCGAG 
ACCGGAAATT GCGGGAATTC GACAATTGGT ATCGCATTCC TGCTCAGCTA TCTCGTGATT 
TCCTTCCTCA TCGTCATCAA CATGTACATC GCTGTGATTT TGGAGAACTA CTCCCAGGCG 
ACGGAGGATG TGCAAGAGGG TCTGACCGAT GATGACTACG ACATGTACTA CGAGATCTGG 
CAGCAGTTCG ACCCTGACGG CACCCAGTAC ATCCGCTACG ACCAACTGTC GGACTTGTTC 
GATGTGTTGG AGCCTCCGCT ACAGATCCAC AAGCCCAACA AGTTCAAAAT CTACTCCATG 
GACATTCCGA TCTGCAAGGG TGACATGATG TTTTGTGTGG ACATTTTGGA CGCGCTAACA 
AAAGACTTCT TCGCTCGCAA GGGCAACGAA ATCGAGGAAA CTGCTGAACT TAGTGAAGTG 
CAACCGAGAC AGAACGAACC GGGTTACGAA CAAGTCAGTT CGACGCTTTA CAGACAGCGC 
GAAGAATATT GTGCGAGACT GATCCAGAAT GCCTGGCGCA AGTATAGACA GCGCACTGGC 
GGTGCTCCAG AGCAAGTGAA TGTCGATGGG GCTGCCGGCG AGGGCGATGG GGAGGTGGAG 
GTCCACCAAA CGGCCGTTCT GGTCGAGAGC GACGGCTTTG TGACCAAGAA CGGGCACAAG 
GTTGTGCTGC ACAGTCGCAC ACCCAGCATC AGCTCCAAGT CTGCGGACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837671.1
CDS	588..6701
Translation	MSVASDYSSEEELSLFRPFTRESLAVIETRIAEEYAKQKELEKKRAEGEIRYEDEDEDEGPQPDPTLEQGLPLPVRLHSTFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALIGLQIYMGVLTQKCIKEFPLDGSWGNLTHENWERFVSNESNWYWDDEKGERPLCGNSSGSGMCKEGYVCLQGYGDNPNYGYTSFDTFGWALLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEANAIREAEQKAKDKQDRADARAAAAVEAAEAAAAAAAAAAAGCDNDQYIIKSSSDYSCHSYESFVGQPNGQDDNNKEKISIRSEGLDSVSEQRRVPTNLSKMRKVSAASLSLPGSPFNLRRGSRGSHQFTLRNNRRMVAPPGDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGVMVLPMYYTNLGSRHSSYTSHASRMSYTSHGDLLGGLGSNGKMMTKESQLRNRSLRNGPAAAPLNHKSTRGVYDGPTGHICESKVKQIDNPFIDSNQRQTVVDMKDVMVLNDIIEQAAVSGGSDRGVSVYYFSANNDDEEEEPTFRERFLAYAIKIIDMFCVWDCCKCWVVIQKFVALLVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATLKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFDRIGRFIRWIKGNVLHIAKLVRFKLTNQISDQPSGEGPSNSWNQDVRCDGGLDISGDEILADGIIKKSPKDRLEVTIGTGMDLTVHGDPKRGKNNINKSKTIGNSILEHGMYIGHLDEDEISNKSYGSHKNRFKEESHKGSVDMLDDHEEKRDASKEELGIGEEETEDDRFDLEKPLTDIVIVGNADDIIIDDYAADCFPDSCYKKFPFLAGDEDSPFWQGWGNLRSKTYRLIENKYFETAVITMILLSSMALALEDVHLQSRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFVIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTTLSYEIIPDVNACKAENYTWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKGGDSLALMMTDAQKKYYNAMKKMGSKKPMKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTKVLDSLNMIFIVIFSTECLMKIFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCKPPNSEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDLFDVLEPPLQIHKPNKFKIYSMDIPICKGDMMFCVDILDALTKDFFARKGNEIEETAELSEVQPRQNEPGYEQVSSTLYRQREEYCARLIQNAWRKYRQRTGGAPEQVNVDGAAGEGDGEVEVHQTAVLVESDGFVTKNGHKVVLHSRTPSISSKSADV

Target ORF information:

RefSeq Version	XM_015982185.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic B (Para), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015982185.1

ORF Insert Sequence:

ATGTCCGTTG CCTCTGACTA TTCGTCCGAG GAGGAGCTCA GCCTATTCCG TCCATTCACT 
CGCGAGTCTC TTGCGGTCAT CGAGACGAGA ATTGCGGAAG AATATGCTAA GCAGAAGGAG 
CTCGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGT 
CCCCAGCCTG ATCCAACTCT GGAGCAGGGG CTGCCCTTGC CAGTACGTCT TCACAGTACC 
TTTCCTCCGG AACTGGCCAG TACACCACTT GAAGACATCG ACCCTTTTTA CAGTAACCAA 
AGGACTTTTG TAGTGGTTAG TAAAGGCAAG GACATTTTCC GGTTTAGCGC CACAAACGCC 
CTGTGGGTAC TCGATCCCTT CAATCCGATC CGTCGAGTGG CCATTTACAT TCTAGTGCAT 
CCTTTGTTTT CTCTTTTTAT CATTACCACA ATCCTCGTTA ACTGTATCCT TATGATCATG 
CCCACAACAC CCACCGTAGA GTCAACCGAA GTGATATTCA CAGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC GAGAGGTTTT ATCCTACAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAACT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTCACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACAAT GTTTTCCCTG TCAGTGTTCG CATTGATAGG TTTACAAATT 
TATATGGGTG TTTTAACACA AAAATGTATC AAAGAATTTC CTCTGGATGG CTCTTGGGGG 
AACCTCACCC ACGAAAACTG GGAGAGATTT GTTAGCAACG AATCCAACTG GTATTGGGAT 
GACGAAAAAG GCGAAAGGCC TCTGTGTGGA AACTCCTCAG GATCGGGAAT GTGCAAAGAG 
GGTTACGTTT GTTTGCAAGG TTACGGTGAT AACCCAAACT ATGGTTACAC CAGCTTTGAT 
ACTTTTGGTT GGGCATTACT TTCCGCCTTC AGATTAATGA CTCAGGATTA TTGGGAGAAT 
TTATACCAAC TGGTTTTAAG ATCAGCCGGT CCTTGGCATA TGCTATTTTT TATCGTGATT 
ATATTTTTGG GTTCGTTTTA TTTAGTTAAC TTGATCTTGG CTATCGTCGC TATGTCGTAC 
GACGAGTTGC AGAAGAAAGC CCAAGATGAG GAAGAAGCTG AAGCTAACGC TATAAGGGAA 
GCCGAGCAAA AGGCAAAGGA CAAGCAAGAT CGGGCGGATG CGAGGGCAGC GGCGGCCGTG 
GAGGCGGCCG AGGCAGCGGC CGCTGCCGCG GCGGCGGCGG CAGCAGGGTG TGACAACGAC 
CAGTACATCA TAAAATCGTC TTCGGACTAC TCGTGTCACA GCTATGAGTC GTTCGTGGGG 
CAACCCAACG GTCAAGACGA CAACAACAAG GAGAAGATTA GCATACGCTC GGAAGGATTA 
GATTCCGTGA GTGAACAGAG AAGAGTTCCT ACTAATCTGA GCAAGATGCG GAAAGTCAGC 
GCTGCCAGTC TCAGCCTCCC CGGCTCTCCC TTCAACCTTC GGCGAGGGTC CCGAGGTAGC 
CACCAGTTCA CCTTGCGGAA CAATAGGCGC ATGGTGGCTC CGCCGGGCGA TCGAAAGCCC 
TTGGTGCTGT CGACCTACCT TGACGCCCAA GAGCACCTCC CCTACGCCGA CGACTCTAAC 
GCCGTCACCC CCATGAGCGA GGAGAACGGC GTCATGGTCC TGCCCATGTA CTACACCAAC 
CTGGGATCAC GTCATTCATC GTACACTTCA CATGCCTCAC GGATGTCATA CACTTCTCAT 
GGAGATTTGT TAGGCGGTTT GGGAAGCAAT GGCAAAATGA TGACGAAGGA GAGCCAGCTC 
AGGAACCGAT CGCTGAGAAA CGGACCAGCG GCCGCTCCCC TAAACCACAA GTCAACGCGA 
GGCGTCTACG ATGGTCCCAC GGGGCACATT TGCGAATCAA AAGTTAAGCA AATTGACAAT 
CCTTTTATCG ATAGCAATCA GCGACAGACA GTCGTGGACA TGAAAGATGT TATGGTGCTG 
AACGACATCA TCGAGCAAGC TGCCGTGAGC GGAGGCAGTG ACCGCGGAGT TTCTGTTTAT 
TATTTCTCAG CCAATAATGA CGACGAAGAG GAAGAGCCGA CGTTCAGGGA ACGCTTCCTG 
GCTTATGCCA TCAAAATCAT TGACATGTTT TGCGTATGGG ATTGTTGTAA ATGTTGGGTC 
GTTATTCAGA AGTTTGTCGC CCTTCTTGTC TTCGACCCAT TCGTCGAGCT TTTTATCACG 
CTGTGCATTG TTGTTAACAC CTTGTTTATG GCATTGGATC ACCACGACAT GGACAAGGAT 
TTGGAAAAGG CTTTGAAGAG CGGAAACTAC TTTTTTACTG CGACTTTTAT GATTGAAGCT 
ACTTTAAAAT TGATTGCAAT GAGTCCGAAA TACTACTTTC AAGAAGGCTG GAACATTTTT 
GATTTTATTA TAGTGGCGTT ATCGTTGCTG GAGCTAGGAC TGGAAGGCGT TCAAGGCTTA 
TCGGTCTTGC GTTCATTTCG ACTGTTACGA GTGTTCAAAC TGGCCAAGTC GTGGCCAACT 
TTAAATTTAC TCATTTCCAT AATGGGTAGA ACGATGGGTG CTTTAGGAAA CTTGACTTTT 
GTGCTCTGTA TTATAATATT TATTTTTGCC GTTATGGGCA TGCAACTGTT TGGGAAAAAT 
TACACAGATA ACGTGGATCG GTTTCCCGAC CACGAGCTCC CCCGCTGGAA CTTCACCGAC 
TTCATGCATT CGTTTATGAT AGTTTTTCGC GTTTTGTGTG GCGAATGGAT CGAGTCCATG 
TGGGACTGTA TGCTGGTGGG CGACGTCTCT TGCATTCCGT TCTTTCTCGC AACTGTCGTG 
ATTGGCAATC TTGTGGTCCT TAATCTTTTC TTAGCCCTGC TTTTGAGTAA CTTTGGATCT 
TCGAGTCTGT CGGCGCCGAC TGCCGACAAT GACACGAATA AAATAGCAGA GGCTTTTGAC 
AGAATCGGCC GCTTTATCAG ATGGATCAAA GGCAATGTTT TACATATCGC AAAACTGGTC 
CGATTCAAGC TCACCAACCA AATTTCCGAC CAGCCGTCAG GTGAGGGACC GTCCAACAGT 
TGGAACCAAG ATGTGAGATG TGATGGTGGT TTAGATATCT CCGGAGACGA AATTTTAGCC 
GACGGTATAA TTAAAAAAAG TCCTAAAGAC CGACTGGAAG TAACAATTGG CACCGGAATG 
GATCTTACCG TGCATGGTGA TCCCAAAAGA GGCAAAAACA ATATTAACAA AAGCAAAACA 
ATCGGAAATT CAATTCTGGA ACATGGAATG TACATCGGTC ACCTGGACGA AGACGAAATT 
AGTAACAAAT CGTACGGTAG TCACAAAAAT CGATTTAAAG AGGAAAGTCA TAAAGGGAGT 
GTTGATATGT TAGACGACCA TGAGGAGAAA CGAGATGCTA GTAAAGAAGA ATTAGGAATT 
GGTGAAGAAG AAACAGAAGA CGACAGATTT GACTTGGAAA AACCACTGAC TGACATTGTG 
ATTGTGGGAA ACGCTGACGA TATAATTATC GACGATTACG CAGCCGACTG CTTTCCCGAT 
TCATGTTACA AGAAGTTTCC GTTTCTTGCA GGCGACGAGG ATTCGCCGTT TTGGCAGGGT 
TGGGGGAATT TAAGGTCGAA GACTTATAGA CTAATAGAGA ACAAATATTT TGAAACGGCC 
GTAATCACGA TGATTTTGTT GAGTAGCATG GCGTTGGCGT TAGAAGACGT ACATTTGCAA 
AGTCGGCCGA TTTTGCAAGA CATACTGTAT TACATGGATC GAATATTCAC CGTGATATTC 
TTTTTCGAGA TGTTGATAAA GTGGTTAGCG TTAGGGTTCC AAAAATACTT TACGAACGCT 
TGGTGCTGGC TAGATTTTGT GATTGTAATG GTGTCTCTCA TTAATTTTAT TGCCTCTCTT 
TGTGGCGCTG GCGGGATTCA AGCATTTAAA ACTATGCGAA CGCTGCGGGC TTTGAGACCA 
CTTCGCGCCA TGTCCCGCAT GCAAGGGATG AGGGTTGTCG TTAATGCATT GGTGCAAGCG 
ATACCGTCTA TCTTCAATGT ACTGCTAGTG TGCTTAATAT TTTGGTTAAT TTTTGCTATA 
ATGGGTGTGC AGTTATTCGC TGGTAAATAC TTTAAGTGCG TGGATGCAAA CAAAACCACA 
CTAAGCTATG AGATTATCCC TGATGTTAAC GCGTGTAAAG CTGAGAATTA CACGTGGGAA 
AACTCCCCAA TGAATTTTGA TCATGTGGGC AAAGCATATT TGTGCTTATT TCAAGTGGCG 
ACATTCAAGG GGTGGATTCA AATTATGAAC GATGCTATAG ATTCTAGAGA CATCAACAAA 
CAACCAATAA GAGAAACAAA CATTTATATG TATTTATACT TCGTATTTTT TATAATATTT 
GGATCGTTTT TTACACTTAA CTTATTCATT GGTGTAATCA TCGATAATTT TAACGAACAA 
AAGAAAAAGG GTGGCGATTC ATTGGCACTG ATGATGACAG ACGCCCAGAA GAAATATTAC 
AATGCGATGA AAAAAATGGG CTCTAAGAAA CCAATGAAGG CGATACCGAG GCCGAGGTGG 
AGACCTCAAG CTATAGTTTT CGAAATAGTA ACAAATAAGA AATTTGATAT GATAATCATG 
TTGTTTATTG GACTGAACAT GCTTACGATG ACAATGGATC ACTACCAACA GAAAGAAACT 
TTCACGAAAG TCCTCGATTC CCTCAACATG ATTTTCATTG TTATCTTTAG TACCGAGTGC 
CTCATGAAAA TTTTTGCGTT ACGTTACCAC TACTTTACGG AACCTTGGAA CCTGTTCGAT 
TTAGTGGTAG TAATATTATC AATTTTGGGT TTGGTTTTGA GTGACATCAT AGAGAAATAT 
TTCGTGTCAC CGACGTTGTT AAGGGTGGTT CGTGTGGCCA AAGTAGGGCG AGTTCTGCGT 
TTGGTGAAAG GTGCAAAGGG CATTCGGACA CTGCTGTTCG CACTAGCCAT GTCACTGCCG 
GCACTGTTCA ACATCTGTTT GTTGCTGTTC CTGGTCATGT TCATTTTTGC CATCTTCGGG 
ATGTCGTTCT TCATGCACGT GAAGGACAAG AGCGGACTTG ACGATGTCTA CAACTTTAAA 
ACGTTTGGCC AGTCGATGAT TCTCCTCTTC CAGATGTCCA CTTCGGCGGG CTGGGATGGA 
GTCCTGGATG GTATTATCAA TGAAGAAGAT TGTAAACCGC CAAACAGTGA GATCGGCGAG 
ACCGGAAATT GCGGGAATTC GACAATTGGT ATCGCATTCC TGCTCAGCTA TCTCGTGATT 
TCCTTCCTCA TCGTCATCAA CATGTACATC GCTGTGATTT TGGAGAACTA CTCCCAGGCG 
ACGGAGGATG TGCAAGAGGG TCTGACCGAT GATGACTACG ACATGTACTA CGAGATCTGG 
CAGCAGTTCG ACCCTGACGG CACCCAGTAC ATCCGCTACG ACCAACTGTC GGACTTGTTC 
GATGTGTTGG AGCCTCCGCT ACAGATCCAC AAGCCCAACA AGTTCAAAAT CTACTCCATG 
GACATTCCGA TCTGCAAGGG TGACATGATG TTTTGTGTGG ACATTTTGGA CGCGCTAACA 
AAAGACTTCT TCGCTCGCAA GGGCAACGAA ATCGAGGAAA CTGCTGAACT TAGTGAAGTG 
CAACCGAGAC AGAACGAACC GGGTTACGAA CAAGTCAGTT CGACGCTTTA CAGACAGCGC 
GAAGAATATT GTGCGAGACT GATCCAGAAT GCCTGGCGCA AGTATAGACA GCGCACTGGC 
GGTGCTCCAG AGCAAGTGAA TGTCGATGGG GCTGCCGGCG AGGGCGATGG GGAGGTGGAG 
GTCCACCAAA CGGCCGTTCT GGTCGAGAGC GACGGCTTTG TGACCAAGAA CGGGCACAAG 
GTTGTGCTGC ACAGTCGCAC ACCCAGCATC AGCTCCAAGT CTGCGGACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Para cDNA ORF clone, Tribolium castaneum(red flour beetle)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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