Para cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	Para
Entrez Gene ID	656391
Full Name	paralytic A
Synonyms	paralytic
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015982050.1	XP_015837536.1	paralytic A isoform X6
XM_015981874.1	XP_015837360.1	paralytic A isoform X1
XM_015982008.1	XP_015837494.1	paralytic A isoform X5
NM_001165908.1	NP_001159380.1	paralytic A
XM_015981938.1	XP_015837424.1	paralytic A isoform X3
XM_015981971.1	XP_015837457.1	paralytic A isoform X4
XM_015981899.1	XP_015837385.1	paralytic A isoform X2

Pathways from BioSystems
Gene Ontology

The following Para gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Para cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg15859	NM_001165908.1 Latest version!	Tribolium castaneum paralytic A (Para), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16165	XM_015981874.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16166	XM_015981899.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16167	XM_015981938.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16168	XM_015981971.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16169	XM_015982008.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16170	XM_015982050.1 Latest version!	Tribolium castaneum paralytic A (Para), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Para ( NM_001165908.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001159380.1 Tribolium castaneum paralytic A (Para), mRNA.

Price & Availability↑

CloneID	OTg15859
Clone ID Related Accession (Same CDS sequence)	NM_001165908.1
Accession Version	NM_001165908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1535644800000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ202024.1. On Sep 29, 2009 this sequence version replaced XM_962927.2. ##Evidence-Data-START## Transcript exon combination :: GQ202024.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1523770, SAMEA1523771 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA ACCGGTTTTG GACGCAGGAA AAAGAAAAAA GAAGATGCAG 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGA CGAACGAGAG TGTGCGTTAG AAATATCAGA AGACGAAATA 
TTGGCTAACG GTTTAATGAA GAAAAACCCT AAAGACCGGG TTGAAGTAAC GATAGGCATA 
GGGACCGGTA TGGACCTTAC AGTTCATGGT GATTCGAAAG CGAATTTGAA ACGAGACAAG 
AACAGCTTGA GTAAAAGCAA AACTATCGGA AATTCAATTT TAGACCACAG TGATTTTATT 
AGTCACTTGG ACGACGACGA AATTAGTAAT AATTCATACG GGAGTCACAC TTATCGCTTT 
AAGGACGAGA GTCACAAAGG CAGTGCCGAC GTTCTTGAAG ATAACGAAGA GCAGGAAGAG 
AAGCGCGATG CTAGCAAAGA AGAGCTAGGT ATCGCCGAAG ACACGGAAGA AGAAGGCTGC 
GAATGTAAGG AAGTCCTGAA TGAAGATTTG GTTGACGCAA ACACTGAAGA CATCATCGAC 
GAATATTCCG CCGACTGCTG TCCCGAGTCG TGTTACAAAA AGTTCCCGTT CCTGGCCGGC 
GACGAAGACT CGCCCTTTTG GCAAGGCTGG GCGAACTTGC GCTACAAAAC GTTCCGGCTG 
ATAGAAAACA AATATTTCGA AACGGCGGTC ATCACGATGA TTTTGCTGAG CAGTCTGGCC 
CTGGCTTTGG AGGATGTGCA TCTTTCGAAA CGGCCGATCT TGCAAGACAT TTTATACTAC 
ATGGACCGCA TTTTTACAGT GATTTTCTTT TTCGAAATGT TGATAAAGTG GCTGGCTTTA 
GGTTTCCAAA AGTACTTTAC GAACGCATGG TGCTGGCTAG ATTTTATTAT CGTAATGGTG 
TCCTTAATTA ATTTTATTGC CTCTCTTTGT GGCGCTGGTG GTATTCAAGC GTTTAAAACC 
ATGAGAACAC TCCGAGCTCT GAGGCCGCTC CGTGCCATGT CGCGGATGCA AGGGATGAGG 
GTTGTGGTGA ACGCACTCGT ACAAGCTATA CCATCCATCT TCAATGTACT GCTAGTGTGC 
TTGATCTTTT GGTTGATTTT TGCTATAATG GGTGTACAAT TATTTGCTGG TAAATACTTT 
AAGTGCGTGG ACGCAAATAA GACGAGTCTG AGCTACGAAA TTATTCCCGA CTACAACGCT 
TGTATTGCCG AAAATTACAC GTGGGAGAAT TCCAGAATGA ATTTTGATCA CGTGGGTAAA 
GCCTACCTTT GCTTGTTTCA AGTCGCCACG TTTAAGGGCT GGATACAAAT CATGAACGAT 
GCGATAGATT CCAGAGAGAT TAATAAACAA CCAATAAGAG AGACGAACAT TTACATGTAT 
TTATATTTCG TCTTCTTTAT TATTTTTGGA TCGTTCTTTA CTTTGAATCT GTTTATTGGG 
GTGATCATTG ATAACTTTAA TGCACAAAAG AAGAAAGCCG GCGGTTCGTT GGAAATGTTC 
ATGACTGAGG ATCAGAAAAA GTACTACAAT GCGATGAAGA AAATGGGTTC GAAGAAGCCT 
ATGAAAGCGA TCCCGAGACC GAGGTGGAAG CCTCAAGCTA TCGTGTTTGA GATAGTAACA 
AATAAGAAAT TTGACATGAT AATCATGTTG TTCATTGGCT TGAACATGTT GACGATGACG 
ATGGACCATT ACCAGCAGAA AGAGACGTTT ACGCAAGTGC TAGACTACCT CAACATGATT 
TTCATAGTTA TATTTAGTAC CGAGTGCCTC ATGAAGATGT TTGCGTTGCG ATACCATTAC 
TTCACGGAAC CGTGGAATCT CTTCGATTTG GTAGTTGTGA TTTTGTCAAT CTTGGGTTTA 
GTTTTGAGTG ATATCATCGA AAAATATTTC GTATCACCAA CACTGTTGAG AGTGGTGCGT 
GTGGCAAAAG TCGGGAGGGT ACTCCGTTTG GTGAAAGGAG CAAAAGGCAT CCGCACACTG 
CTTTTCGCAT TAGCCATGTC ACTGCCTGCA CTATTCAACA TCTGTTTACT GTTATTCTTG 
GTGATGTTCA TCTTTGCCAT CTTCGGGATG TCCTTCTTCA TGCACGTCAA AGACAAGAGC 
GGATTGGATG ACGTCTACAA CTTCAAAACT TTTGCCCAGT CCATGATTCT CCTATTTCAG 
ATGTCCACTT CGGCCGGGTG GGACGGCGTT TTGGACGGGA TTATAAACGA AGAGGACTGC 
AAGTTGCCGA ACAATGAGAT AGGAGAGACG GGGAACTGCG GGAACTCAAC AATAGGCATC 
GCCTTTCTAC TTAGCTACCT CGTGATCTCT TTCCTGATCG TCATCAACAT GTACATCGCC 
GTGATCTTGG AGAACTACTC CCAGGCCACG GAAGACGTGC AGGAGGGCTT GACCGACGAC 
GACTACGACA TGTACTACGA GATCTGGCAG CAGTTCGACC CTGACGGCAC CCAGTACATC 
CGCTACGACC AGCTGTCGGA CTTCCTGGAT GTGCTGGAGC CGCCTTTGCA AATCCACAAA 
CCCAACAAAT ACAAGATAGT TTCCATGGAC ATCACCATAT GCAAGGGTGA TCTCATGTTT 
TGTGTAGATA TTTTAGATGC GCTAACTAAA GACTTCTTCG CCCGCAAGGG CAATCCGATT 
GAAGAGACCG CCGAACTCAG TGAAGTGAAC ACTCACCCGA ACGAGCCGGG CTACGAACCA 
GTGAGCTCCA CGCTGTGGAG GCAGCGCGAG GAGTACTGTG CGCGCCTGAT CCAGAACGCG 
TGGCGCAAGC ACAAGCAGCA GCGCAGCAGC GGCGTGCCCG AGCCGGCAGG CGAGGGGGAG 
CTGGACAAAG ACAGTGAGCT GGAGGCGCAC CAAACGGCCG TTTTGGTGGA GAACGACGGC 
TTTGCGACCA AAAACGGGCA CAAAGTGGTG TTGCACAGTC GCACGCCCAG CGTCAGCTCC 
AGGTCTGCGG ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159380.1
CDS	1..6138
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEPVLDAGKRKKKMQIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSVSEHRRVPNLSKIRKVSAASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	NM_001165908.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165908.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA ACCGGTTTTG GACGCAGGAA AAAGAAAAAA GAAGATGCAG 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGA CGAACGAGAG TGTGCGTTAG AAATATCAGA AGACGAAATA 
TTGGCTAACG GTTTAATGAA GAAAAACCCT AAAGACCGGG TTGAAGTAAC GATAGGCATA 
GGGACCGGTA TGGACCTTAC AGTTCATGGT GATTCGAAAG CGAATTTGAA ACGAGACAAG 
AACAGCTTGA GTAAAAGCAA AACTATCGGA AATTCAATTT TAGACCACAG TGATTTTATT 
AGTCACTTGG ACGACGACGA AATTAGTAAT AATTCATACG GGAGTCACAC TTATCGCTTT 
AAGGACGAGA GTCACAAAGG CAGTGCCGAC GTTCTTGAAG ATAACGAAGA GCAGGAAGAG 
AAGCGCGATG CTAGCAAAGA AGAGCTAGGT ATCGCCGAAG ACACGGAAGA AGAAGGCTGC 
GAATGTAAGG AAGTCCTGAA TGAAGATTTG GTTGACGCAA ACACTGAAGA CATCATCGAC 
GAATATTCCG CCGACTGCTG TCCCGAGTCG TGTTACAAAA AGTTCCCGTT CCTGGCCGGC 
GACGAAGACT CGCCCTTTTG GCAAGGCTGG GCGAACTTGC GCTACAAAAC GTTCCGGCTG 
ATAGAAAACA AATATTTCGA AACGGCGGTC ATCACGATGA TTTTGCTGAG CAGTCTGGCC 
CTGGCTTTGG AGGATGTGCA TCTTTCGAAA CGGCCGATCT TGCAAGACAT TTTATACTAC 
ATGGACCGCA TTTTTACAGT GATTTTCTTT TTCGAAATGT TGATAAAGTG GCTGGCTTTA 
GGTTTCCAAA AGTACTTTAC GAACGCATGG TGCTGGCTAG ATTTTATTAT CGTAATGGTG 
TCCTTAATTA ATTTTATTGC CTCTCTTTGT GGCGCTGGTG GTATTCAAGC GTTTAAAACC 
ATGAGAACAC TCCGAGCTCT GAGGCCGCTC CGTGCCATGT CGCGGATGCA AGGGATGAGG 
GTTGTGGTGA ACGCACTCGT ACAAGCTATA CCATCCATCT TCAATGTACT GCTAGTGTGC 
TTGATCTTTT GGTTGATTTT TGCTATAATG GGTGTACAAT TATTTGCTGG TAAATACTTT 
AAGTGCGTGG ACGCAAATAA GACGAGTCTG AGCTACGAAA TTATTCCCGA CTACAACGCT 
TGTATTGCCG AAAATTACAC GTGGGAGAAT TCCAGAATGA ATTTTGATCA CGTGGGTAAA 
GCCTACCTTT GCTTGTTTCA AGTCGCCACG TTTAAGGGCT GGATACAAAT CATGAACGAT 
GCGATAGATT CCAGAGAGAT TAATAAACAA CCAATAAGAG AGACGAACAT TTACATGTAT 
TTATATTTCG TCTTCTTTAT TATTTTTGGA TCGTTCTTTA CTTTGAATCT GTTTATTGGG 
GTGATCATTG ATAACTTTAA TGCACAAAAG AAGAAAGCCG GCGGTTCGTT GGAAATGTTC 
ATGACTGAGG ATCAGAAAAA GTACTACAAT GCGATGAAGA AAATGGGTTC GAAGAAGCCT 
ATGAAAGCGA TCCCGAGACC GAGGTGGAAG CCTCAAGCTA TCGTGTTTGA GATAGTAACA 
AATAAGAAAT TTGACATGAT AATCATGTTG TTCATTGGCT TGAACATGTT GACGATGACG 
ATGGACCATT ACCAGCAGAA AGAGACGTTT ACGCAAGTGC TAGACTACCT CAACATGATT 
TTCATAGTTA TATTTAGTAC CGAGTGCCTC ATGAAGATGT TTGCGTTGCG ATACCATTAC 
TTCACGGAAC CGTGGAATCT CTTCGATTTG GTAGTTGTGA TTTTGTCAAT CTTGGGTTTA 
GTTTTGAGTG ATATCATCGA AAAATATTTC GTATCACCAA CACTGTTGAG AGTGGTGCGT 
GTGGCAAAAG TCGGGAGGGT ACTCCGTTTG GTGAAAGGAG CAAAAGGCAT CCGCACACTG 
CTTTTCGCAT TAGCCATGTC ACTGCCTGCA CTATTCAACA TCTGTTTACT GTTATTCTTG 
GTGATGTTCA TCTTTGCCAT CTTCGGGATG TCCTTCTTCA TGCACGTCAA AGACAAGAGC 
GGATTGGATG ACGTCTACAA CTTCAAAACT TTTGCCCAGT CCATGATTCT CCTATTTCAG 
ATGTCCACTT CGGCCGGGTG GGACGGCGTT TTGGACGGGA TTATAAACGA AGAGGACTGC 
AAGTTGCCGA ACAATGAGAT AGGAGAGACG GGGAACTGCG GGAACTCAAC AATAGGCATC 
GCCTTTCTAC TTAGCTACCT CGTGATCTCT TTCCTGATCG TCATCAACAT GTACATCGCC 
GTGATCTTGG AGAACTACTC CCAGGCCACG GAAGACGTGC AGGAGGGCTT GACCGACGAC 
GACTACGACA TGTACTACGA GATCTGGCAG CAGTTCGACC CTGACGGCAC CCAGTACATC 
CGCTACGACC AGCTGTCGGA CTTCCTGGAT GTGCTGGAGC CGCCTTTGCA AATCCACAAA 
CCCAACAAAT ACAAGATAGT TTCCATGGAC ATCACCATAT GCAAGGGTGA TCTCATGTTT 
TGTGTAGATA TTTTAGATGC GCTAACTAAA GACTTCTTCG CCCGCAAGGG CAATCCGATT 
GAAGAGACCG CCGAACTCAG TGAAGTGAAC ACTCACCCGA ACGAGCCGGG CTACGAACCA 
GTGAGCTCCA CGCTGTGGAG GCAGCGCGAG GAGTACTGTG CGCGCCTGAT CCAGAACGCG 
TGGCGCAAGC ACAAGCAGCA GCGCAGCAGC GGCGTGCCCG AGCCGGCAGG CGAGGGGGAG 
CTGGACAAAG ACAGTGAGCT GGAGGCGCAC CAAACGGCCG TTTTGGTGGA GAACGACGGC 
TTTGCGACCA AAAACGGGCA CAAAGTGGTG TTGCACAGTC GCACGCCCAG CGTCAGCTCC 
AGGTCTGCGG ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015981874.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837360.1 Tribolium castaneum paralytic A (Para), transcript variant X1, mRNA.

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CloneID	OTg16165
Clone ID Related Accession (Same CDS sequence)	XM_015981874.1
Accession Version	XM_015981874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGG TAAAAAAAGA AACTTATCTC TAGTTAGTCA CTTGGTAATT 
TCAGACGAAC GAGAGTGTGC GTTAGAAATA TCAGAAGACG AAATATTGGC TAACGGTTTA 
ATGAAGAAAA ACCCTAAAGA CCGGGTTGAA GTAACGATAG GCATAGGGAC CGGTATGGAC 
CTTACAGTTC ATGGTGATTC GAAAGCGAAT TTGAAACGAG ACAAGAACAG CTTGAGTAAA 
AGCAAAACTA TCGGAAATTC AATTTTAGAC CACAGTGATT TTATTAGTCA CTTGGACGAC 
GACGAAATTA GTAATAATTC ATACGGGAGT CACACTTATC GCTTTAAGGA CGAGAGTCAC 
AAAGGCAGTG CCGACGTTCT TGAAGATAAC GAAGAGCAGG AAGAGAAGCG CGATGCTAGC 
AAAGAAGAGC TAGGTATCGC CGAAGACACG GAAGAAGAAG GCTGCGAATG TAAGGAAGTC 
CTGAATGAAG ATTTGGTTGA CGCAAACACT GAAGACATCA TCGACGAATA TTCCGCCGAC 
TGCTGTCCCG AGTCGTGTTA CAAAAAGTTC CCGTTCCTGG CCGGCGACGA AGACTCGCCC 
TTTTGGCAAG GCTGGGCGAA CTTGCGCTAC AAAACGTTCC GGCTGATAGA AAACAAATAT 
TTCGAAACGG CGGTCATCAC GATGATTTTG CTGAGCAGTC TGGCCCTGGC TTTGGAGGAT 
GTGCATCTTT CGAAACGGCC GATCTTGCAA GACATTTTAT ACTACATGGA CCGCATTTTT 
ACAGTGATTT TCTTTTTCGA AATGTTGATA AAGTGGCTGG CTTTAGGTTT CCAAAAGTAC 
TTTACGAACG CATGGTGCTG GCTAGATTTT ATTATCGTAA TGGTGTCCTT AATTAATTTT 
ATTGCCTCTC TTTGTGGCGC TGGTGGTATT CAAGCGTTTA AAACCATGAG AACACTCCGA 
GCTCTGAGGC CGCTCCGTGC CATGTCGCGG ATGCAAGGGA TGAGGGTTGT GGTGAACGCA 
CTCGTACAAG CTATACCATC CATCTTCAAT GTACTGCTAG TGTGCTTGAT CTTTTGGTTG 
ATTTTTGCTA TAATGGGTGT ACAATTATTT GCTGGTAAAT ACTTTAAGTG CGTGGACGCA 
AATAAGACGA GTCTGAGCTA CGAAATTATT CCCGACTACA ACGCTTGTAT TGCCGAAAAT 
TACACGTGGG AGAATTCCAG AATGAATTTT GATCACGTGG GTAAAGCCTA CCTTTGCTTG 
TTTCAAGTCG CCACGTTTAA GGGCTGGATA CAAATCATGA ACGATGCGAT AGATTCCAGA 
GAGATTAATA AACAACCAAT AAGAGAGACG AACATTTACA TGTATTTATA TTTCGTCTTC 
TTTATTATTT TTGGATCGTT CTTTACTTTG AATCTGTTTA TTGGGGTGAT CATTGATAAC 
TTTAATGCAC AAAAGAAGAA AGCCGGCGGT TCGTTGGAAA TGTTCATGAC TGAGGATCAG 
AAAAAGTACT ACAATGCGAT GAAGAAAATG GGTTCGAAGA AGCCTATGAA AGCGATCCCG 
AGACCGAGGT GGAAGCCTCA AGCTATCGTG TTTGAGATAG TAACAAATAA GAAATTTGAC 
ATGATAATCA TGTTGTTCAT TGGCTTGAAC ATGTTGACGA TGACGATGGA CCATTACCAG 
CAGAAAGAGA CGTTTACGCA AGTGCTAGAC TACCTCAACA TGATTTTCAT AGTTATATTT 
AGTACCGAGT GCCTCATGAA GATGTTTGCG TTGCGATACC ATTACTTCAC GGAACCGTGG 
AATCTCTTCG ATTTGGTAGT TGTGATTTTG TCAATCTTGG GTTTAGTTTT GAGTGATATC 
ATCGAAAAAT ATTTCGTATC ACCAACACTG TTGAGAGTGG TGCGTGTGGC AAAAGTCGGG 
AGGGTACTCC GTTTGGTGAA AGGAGCAAAA GGCATCCGCA CACTGCTTTT CGCATTAGCC 
ATGTCACTGC CTGCACTATT CAACATCTGT TTACTGTTAT TCTTGGTGAT GTTCATCTTT 
GCCATCTTCG GGATGTCCTT CTTCATGCAC GTCAAAGACA AGAGCGGATT GGATGACGTC 
TACAACTTCA AAACTTTTGC CCAGTCCATG ATTCTCCTAT TTCAGATGTC CACTTCGGCC 
GGGTGGGACG GCGTTTTGGA CGGGATTATA AACGAAGAGG ACTGCAAGTT GCCGAACAAT 
GAGATAGGAG AGACGGGGAA CTGCGGGAAC TCAACAATAG GCATCGCCTT TCTACTTAGC 
TACCTCGTGA TCTCTTTCCT GATCGTCATC AACATGTACA TCGCCGTGAT CTTGGAGAAC 
TACTCCCAGG CCACGGAAGA CGTGCAGGAG GGCTTGACCG ACGACGACTA CGACATGTAC 
TACGAGATCT GGCAGCAGTT CGACCCTGAC GGCACCCAGT ACATCCGCTA CGACCAGCTG 
TCGGACTTCC TGGATGTGCT GGAGCCGCCT TTGCAAATCC ACAAACCCAA CAAATACAAG 
ATAGTTTCCA TGGACATCAC CATATGCAAG GGTGATCTCA TGTTTTGTGT AGATATTTTA 
GATGCGCTAA CTAAAGACTT CTTCGCCCGC AAGGGCAATC CGATTGAAGA GACCGCCGAA 
CTCAGTGAAG TGAACACTCA CCCGAACGAG CCGGGCTACG AACCAGTGAG CTCCACGCTG 
TGGAGGCAGC GCGAGGAGTA CTGTGCGCGC CTGATCCAGA ACGCGTGGCG CAAGCACAAG 
CAGCAGCGCA GCAGCGGCGT GCCCGAGCCG GCAGGCGAGG GGGAGCTGGA CAAAGACAGT 
GAGCTGGAGG CGCACCAAAC GGCCGTTTTG GTGGAGAACG ACGGCTTTGC GACCAAAAAC 
GGGCACAAAG TGGTGTTGCA CAGTCGCACG CCCAGCGTCA GCTCCAGGTC TGCGGACGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837360.1
CDS	2126..8308
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGETGFGRRKKKKEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSVSEHRRVPNLSKIRKVSAASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSGKKRNLSLVSHLVISDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015981874.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015981874.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGG TAAAAAAAGA AACTTATCTC TAGTTAGTCA CTTGGTAATT 
TCAGACGAAC GAGAGTGTGC GTTAGAAATA TCAGAAGACG AAATATTGGC TAACGGTTTA 
ATGAAGAAAA ACCCTAAAGA CCGGGTTGAA GTAACGATAG GCATAGGGAC CGGTATGGAC 
CTTACAGTTC ATGGTGATTC GAAAGCGAAT TTGAAACGAG ACAAGAACAG CTTGAGTAAA 
AGCAAAACTA TCGGAAATTC AATTTTAGAC CACAGTGATT TTATTAGTCA CTTGGACGAC 
GACGAAATTA GTAATAATTC ATACGGGAGT CACACTTATC GCTTTAAGGA CGAGAGTCAC 
AAAGGCAGTG CCGACGTTCT TGAAGATAAC GAAGAGCAGG AAGAGAAGCG CGATGCTAGC 
AAAGAAGAGC TAGGTATCGC CGAAGACACG GAAGAAGAAG GCTGCGAATG TAAGGAAGTC 
CTGAATGAAG ATTTGGTTGA CGCAAACACT GAAGACATCA TCGACGAATA TTCCGCCGAC 
TGCTGTCCCG AGTCGTGTTA CAAAAAGTTC CCGTTCCTGG CCGGCGACGA AGACTCGCCC 
TTTTGGCAAG GCTGGGCGAA CTTGCGCTAC AAAACGTTCC GGCTGATAGA AAACAAATAT 
TTCGAAACGG CGGTCATCAC GATGATTTTG CTGAGCAGTC TGGCCCTGGC TTTGGAGGAT 
GTGCATCTTT CGAAACGGCC GATCTTGCAA GACATTTTAT ACTACATGGA CCGCATTTTT 
ACAGTGATTT TCTTTTTCGA AATGTTGATA AAGTGGCTGG CTTTAGGTTT CCAAAAGTAC 
TTTACGAACG CATGGTGCTG GCTAGATTTT ATTATCGTAA TGGTGTCCTT AATTAATTTT 
ATTGCCTCTC TTTGTGGCGC TGGTGGTATT CAAGCGTTTA AAACCATGAG AACACTCCGA 
GCTCTGAGGC CGCTCCGTGC CATGTCGCGG ATGCAAGGGA TGAGGGTTGT GGTGAACGCA 
CTCGTACAAG CTATACCATC CATCTTCAAT GTACTGCTAG TGTGCTTGAT CTTTTGGTTG 
ATTTTTGCTA TAATGGGTGT ACAATTATTT GCTGGTAAAT ACTTTAAGTG CGTGGACGCA 
AATAAGACGA GTCTGAGCTA CGAAATTATT CCCGACTACA ACGCTTGTAT TGCCGAAAAT 
TACACGTGGG AGAATTCCAG AATGAATTTT GATCACGTGG GTAAAGCCTA CCTTTGCTTG 
TTTCAAGTCG CCACGTTTAA GGGCTGGATA CAAATCATGA ACGATGCGAT AGATTCCAGA 
GAGATTAATA AACAACCAAT AAGAGAGACG AACATTTACA TGTATTTATA TTTCGTCTTC 
TTTATTATTT TTGGATCGTT CTTTACTTTG AATCTGTTTA TTGGGGTGAT CATTGATAAC 
TTTAATGCAC AAAAGAAGAA AGCCGGCGGT TCGTTGGAAA TGTTCATGAC TGAGGATCAG 
AAAAAGTACT ACAATGCGAT GAAGAAAATG GGTTCGAAGA AGCCTATGAA AGCGATCCCG 
AGACCGAGGT GGAAGCCTCA AGCTATCGTG TTTGAGATAG TAACAAATAA GAAATTTGAC 
ATGATAATCA TGTTGTTCAT TGGCTTGAAC ATGTTGACGA TGACGATGGA CCATTACCAG 
CAGAAAGAGA CGTTTACGCA AGTGCTAGAC TACCTCAACA TGATTTTCAT AGTTATATTT 
AGTACCGAGT GCCTCATGAA GATGTTTGCG TTGCGATACC ATTACTTCAC GGAACCGTGG 
AATCTCTTCG ATTTGGTAGT TGTGATTTTG TCAATCTTGG GTTTAGTTTT GAGTGATATC 
ATCGAAAAAT ATTTCGTATC ACCAACACTG TTGAGAGTGG TGCGTGTGGC AAAAGTCGGG 
AGGGTACTCC GTTTGGTGAA AGGAGCAAAA GGCATCCGCA CACTGCTTTT CGCATTAGCC 
ATGTCACTGC CTGCACTATT CAACATCTGT TTACTGTTAT TCTTGGTGAT GTTCATCTTT 
GCCATCTTCG GGATGTCCTT CTTCATGCAC GTCAAAGACA AGAGCGGATT GGATGACGTC 
TACAACTTCA AAACTTTTGC CCAGTCCATG ATTCTCCTAT TTCAGATGTC CACTTCGGCC 
GGGTGGGACG GCGTTTTGGA CGGGATTATA AACGAAGAGG ACTGCAAGTT GCCGAACAAT 
GAGATAGGAG AGACGGGGAA CTGCGGGAAC TCAACAATAG GCATCGCCTT TCTACTTAGC 
TACCTCGTGA TCTCTTTCCT GATCGTCATC AACATGTACA TCGCCGTGAT CTTGGAGAAC 
TACTCCCAGG CCACGGAAGA CGTGCAGGAG GGCTTGACCG ACGACGACTA CGACATGTAC 
TACGAGATCT GGCAGCAGTT CGACCCTGAC GGCACCCAGT ACATCCGCTA CGACCAGCTG 
TCGGACTTCC TGGATGTGCT GGAGCCGCCT TTGCAAATCC ACAAACCCAA CAAATACAAG 
ATAGTTTCCA TGGACATCAC CATATGCAAG GGTGATCTCA TGTTTTGTGT AGATATTTTA 
GATGCGCTAA CTAAAGACTT CTTCGCCCGC AAGGGCAATC CGATTGAAGA GACCGCCGAA 
CTCAGTGAAG TGAACACTCA CCCGAACGAG CCGGGCTACG AACCAGTGAG CTCCACGCTG 
TGGAGGCAGC GCGAGGAGTA CTGTGCGCGC CTGATCCAGA ACGCGTGGCG CAAGCACAAG 
CAGCAGCGCA GCAGCGGCGT GCCCGAGCCG GCAGGCGAGG GGGAGCTGGA CAAAGACAGT 
GAGCTGGAGG CGCACCAAAC GGCCGTTTTG GTGGAGAACG ACGGCTTTGC GACCAAAAAC 
GGGCACAAAG TGGTGTTGCA CAGTCGCACG CCCAGCGTCA GCTCCAGGTC TGCGGACGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015981899.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837385.1 Tribolium castaneum paralytic A (Para), transcript variant X2, mRNA.

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CloneID	OTg16166
Clone ID Related Accession (Same CDS sequence)	XM_015981899.1
Accession Version	XM_015981899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGA CGAACGAGAG TGTGCGTTAG AAATATCAGA AGACGAAATA 
TTGGCTAACG GTTTAATGAA GAAAAACCCT AAAGACCGGG TTGAAGTAAC GATAGGCATA 
GGGACCGGTA TGGACCTTAC AGTTCATGGT GATTCGAAAG CGAATTTGAA ACGAGACAAG 
AACAGCTTGA GTAAAAGCAA AACTATCGGA AATTCAATTT TAGACCACAG TGATTTTATT 
AGTCACTTGG ACGACGACGA AATTAGTAAT AATTCATACG GGAGTCACAC TTATCGCTTT 
AAGGACGAGA GTCACAAAGG CAGTGCCGAC GTTCTTGAAG ATAACGAAGA GCAGGAAGAG 
AAGCGCGATG CTAGCAAAGA AGAGCTAGGT ATCGCCGAAG ACACGGAAGA AGAAGGCTGC 
GAATGTAAGG AAGTCCTGAA TGAAGATTTG GTTGACGCAA ACACTGAAGA CATCATCGAC 
GAATATTCCG CCGACTGCTG TCCCGAGTCG TGTTACAAAA AGTTCCCGTT CCTGGCCGGC 
GACGAAGACT CGCCCTTTTG GCAAGGCTGG GCGAACTTGC GCTACAAAAC GTTCCGGCTG 
ATAGAAAACA AATATTTCGA AACGGCGGTC ATCACGATGA TTTTGCTGAG CAGTCTGGCC 
CTGGCTTTGG AGGATGTGCA TCTTTCGAAA CGGCCGATCT TGCAAGACAT TTTATACTAC 
ATGGACCGCA TTTTTACAGT GATTTTCTTT TTCGAAATGT TGATAAAGTG GCTGGCTTTA 
GGTTTCCAAA AGTACTTTAC GAACGCATGG TGCTGGCTAG ATTTTATTAT CGTAATGGTG 
TCCTTAATTA ATTTTATTGC CTCTCTTTGT GGCGCTGGTG GTATTCAAGC GTTTAAAACC 
ATGAGAACAC TCCGAGCTCT GAGGCCGCTC CGTGCCATGT CGCGGATGCA AGGGATGAGG 
GTTGTGGTGA ACGCACTCGT ACAAGCTATA CCATCCATCT TCAATGTACT GCTAGTGTGC 
TTGATCTTTT GGTTGATTTT TGCTATAATG GGTGTACAAT TATTTGCTGG TAAATACTTT 
AAGTGCGTGG ACGCAAATAA GACGAGTCTG AGCTACGAAA TTATTCCCGA CTACAACGCT 
TGTATTGCCG AAAATTACAC GTGGGAGAAT TCCAGAATGA ATTTTGATCA CGTGGGTAAA 
GCCTACCTTT GCTTGTTTCA AGTCGCCACG TTTAAGGGCT GGATACAAAT CATGAACGAT 
GCGATAGATT CCAGAGAGAT TAATAAACAA CCAATAAGAG AGACGAACAT TTACATGTAT 
TTATATTTCG TCTTCTTTAT TATTTTTGGA TCGTTCTTTA CTTTGAATCT GTTTATTGGG 
GTGATCATTG ATAACTTTAA TGCACAAAAG AAGAAAGCCG GCGGTTCGTT GGAAATGTTC 
ATGACTGAGG ATCAGAAAAA GTACTACAAT GCGATGAAGA AAATGGGTTC GAAGAAGCCT 
ATGAAAGCGA TCCCGAGACC GAGGTGGAAG CCTCAAGCTA TCGTGTTTGA GATAGTAACA 
AATAAGAAAT TTGACATGAT AATCATGTTG TTCATTGGCT TGAACATGTT GACGATGACG 
ATGGACCATT ACCAGCAGAA AGAGACGTTT ACGCAAGTGC TAGACTACCT CAACATGATT 
TTCATAGTTA TATTTAGTAC CGAGTGCCTC ATGAAGATGT TTGCGTTGCG ATACCATTAC 
TTCACGGAAC CGTGGAATCT CTTCGATTTG GTAGTTGTGA TTTTGTCAAT CTTGGGTTTA 
GTTTTGAGTG ATATCATCGA AAAATATTTC GTATCACCAA CACTGTTGAG AGTGGTGCGT 
GTGGCAAAAG TCGGGAGGGT ACTCCGTTTG GTGAAAGGAG CAAAAGGCAT CCGCACACTG 
CTTTTCGCAT TAGCCATGTC ACTGCCTGCA CTATTCAACA TCTGTTTACT GTTATTCTTG 
GTGATGTTCA TCTTTGCCAT CTTCGGGATG TCCTTCTTCA TGCACGTCAA AGACAAGAGC 
GGATTGGATG ACGTCTACAA CTTCAAAACT TTTGCCCAGT CCATGATTCT CCTATTTCAG 
ATGTCCACTT CGGCCGGGTG GGACGGCGTT TTGGACGGGA TTATAAACGA AGAGGACTGC 
AAGTTGCCGA ACAATGAGAT AGGAGAGACG GGGAACTGCG GGAACTCAAC AATAGGCATC 
GCCTTTCTAC TTAGCTACCT CGTGATCTCT TTCCTGATCG TCATCAACAT GTACATCGCC 
GTGATCTTGG AGAACTACTC CCAGGCCACG GAAGACGTGC AGGAGGGCTT GACCGACGAC 
GACTACGACA TGTACTACGA GATCTGGCAG CAGTTCGACC CTGACGGCAC CCAGTACATC 
CGCTACGACC AGCTGTCGGA CTTCCTGGAT GTGCTGGAGC CGCCTTTGCA AATCCACAAA 
CCCAACAAAT ACAAGATAGT TTCCATGGAC ATCACCATAT GCAAGGGTGA TCTCATGTTT 
TGTGTAGATA TTTTAGATGC GCTAACTAAA GACTTCTTCG CCCGCAAGGG CAATCCGATT 
GAAGAGACCG CCGAACTCAG TGAAGTGAAC ACTCACCCGA ACGAGCCGGG CTACGAACCA 
GTGAGCTCCA CGCTGTGGAG GCAGCGCGAG GAGTACTGTG CGCGCCTGAT CCAGAACGCG 
TGGCGCAAGC ACAAGCAGCA GCGCAGCAGC GGCGTGCCCG AGCCGGCAGG CGAGGGGGAG 
CTGGACAAAG ACAGTGAGCT GGAGGCGCAC CAAACGGCCG TTTTGGTGGA GAACGACGGC 
TTTGCGACCA AAAACGGGCA CAAAGTGGTG TTGCACAGTC GCACGCCCAG CGTCAGCTCC 
AGGTCTGCGG ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837385.1
CDS	2126..8263
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGETGFGRRKKKKEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSVSEHRRVPNLSKIRKVSAASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015981899.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015981899.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGTGAGTGAA CACAGAAGAG TTCCTAATCT CAGCAAGATA 
CGGAAAGTCA GCGCTGCAAG TCTCAGTCTT CCGGGATCTC CGTTCAACCT TCGCAGAGGG 
TCCAGAGGCA GCCACCAGTT CACCATCCGG AACAACAGGC ACATGGTGGG CGGCCCCTGC 
GACCGCAAAC CTTTGGTTCT CTCGACCTAT CTCGATGCTC AAGAACATCT TCCCTACGCC 
GACGACTCCA ACGCTGTCAC TCCCATGAGC GAGGAGAACG GCGCCATGGT GTTCCCCATG 
TACTACGCCA ATCTGGGGTC ACGTCACTCA TCGTACAACT CCCACGCCGC GCGGATGTCC 
TACACCTCGC ACGGGGACCT CCTTGGAGGC CTCGTAGGCA GTGGGAAAAA CATGACGAAG 
GAGAGCCAAC TCCGGTGCCG TTCCATGAGG AACGCACCTC CGGCCACAAC AAACTCGTTC 
TTTGAAATCA CACACAAAGC ACACCGAGGT GATTATGATG GTCCCACGGG TCAGCTTTGT 
GAATCGAAAT TAAAACAACT GGATAGTTCC TTTATTGACA ACAATCAGAG GCAAACGGTT 
GTAGATATGA AAGACGTAAA AGTCCTCAAC GATATTATTG AGCAAGCGGC TGCCAGCGGC 
GGGAGTGAGC ATGGAGTTTC CGTTTATATT TTCTCAGCGA CTAATAACGA CGAGAATGAC 
GAAGAAGAGG AGGAGCCCAC GGTCGGCGAA CGCATGCTGG CCTACACCCT CAAAATAATC 
GACATTTTCT GCGTCTGGGA CTGTTGCCAC TGCTGGGTCG TCATCCAGAA GTTCGTCAGT 
TTGATAGTGT TTGACCCGTT CGTCGAGCTG TTCATCACGC TCTGCATCGT CGTCAACACC 
CTGTTCATGG CGCTCGACCA CCACGACATG GACAAAGACC TGGAAAAGGC CCTAAAGAGC 
GGCAACTACT TCTTCACCGC CACTTTCATG ATCGAAGCTA CGATGAAGTT GATCGCGATG 
AGTCCGAAGT TCTACTTTCA AGAAGGCTGG AATATCTTTG ATTTCATAAT AGTGGCGTTG 
TCGTTGCTGG AACTTGGGCT TGAAGGGGTC CAGGGGTTGT CCGTTTTGAG ATCATTCCGA 
CTGTTGAGGG TTTTTAAACT GGCAAAGTCG TGGCCAACCT TGAACTTACT CATCTCCATT 
ATGGGCAGGA CGATGGGGGC TTTGGGGAAT CTAACGTTTG TCTTGTGCAT CATCATATTC 
ATATTTGCCG TAATGGGGAT GCAACTATTT GGGAAAAACT ATACAGATAA CGTTGATAGA 
TTTCCCGACC ACGAACTTCC AAGGTGGAAC TTTACCGATT TTATGCATTC CTTTATGATA 
GTGTTTAGAG TACTGTGTGG AGAGTGGATA GAATCTATGT GGGATTGCAT GTTAGTAGGT 
GATGTGTCTT GTATTCCATT CTTCCTAGCA ACTGTGGTAA TTGGTAATCT TGTGGTACTG 
AACCTCTTCT TGGCTCTGCT TTTGAGCAAC TTTGGCTCTT CAAACCTCTC AGCACCGACT 
GCCGACAACG ACACTAATAA AATAGCAGAG GCCTTTGAGA GAATAGCGCG TTTTATACGG 
TGGATAAAGG GTAGTGTTTT AGACATGGTA AAAATCGTCC GATTTAAACT AACTAATCAG 
ATATCAGACC AGCCCTCAGA CGAACGAGAG TGTGCGTTAG AAATATCAGA AGACGAAATA 
TTGGCTAACG GTTTAATGAA GAAAAACCCT AAAGACCGGG TTGAAGTAAC GATAGGCATA 
GGGACCGGTA TGGACCTTAC AGTTCATGGT GATTCGAAAG CGAATTTGAA ACGAGACAAG 
AACAGCTTGA GTAAAAGCAA AACTATCGGA AATTCAATTT TAGACCACAG TGATTTTATT 
AGTCACTTGG ACGACGACGA AATTAGTAAT AATTCATACG GGAGTCACAC TTATCGCTTT 
AAGGACGAGA GTCACAAAGG CAGTGCCGAC GTTCTTGAAG ATAACGAAGA GCAGGAAGAG 
AAGCGCGATG CTAGCAAAGA AGAGCTAGGT ATCGCCGAAG ACACGGAAGA AGAAGGCTGC 
GAATGTAAGG AAGTCCTGAA TGAAGATTTG GTTGACGCAA ACACTGAAGA CATCATCGAC 
GAATATTCCG CCGACTGCTG TCCCGAGTCG TGTTACAAAA AGTTCCCGTT CCTGGCCGGC 
GACGAAGACT CGCCCTTTTG GCAAGGCTGG GCGAACTTGC GCTACAAAAC GTTCCGGCTG 
ATAGAAAACA AATATTTCGA AACGGCGGTC ATCACGATGA TTTTGCTGAG CAGTCTGGCC 
CTGGCTTTGG AGGATGTGCA TCTTTCGAAA CGGCCGATCT TGCAAGACAT TTTATACTAC 
ATGGACCGCA TTTTTACAGT GATTTTCTTT TTCGAAATGT TGATAAAGTG GCTGGCTTTA 
GGTTTCCAAA AGTACTTTAC GAACGCATGG TGCTGGCTAG ATTTTATTAT CGTAATGGTG 
TCCTTAATTA ATTTTATTGC CTCTCTTTGT GGCGCTGGTG GTATTCAAGC GTTTAAAACC 
ATGAGAACAC TCCGAGCTCT GAGGCCGCTC CGTGCCATGT CGCGGATGCA AGGGATGAGG 
GTTGTGGTGA ACGCACTCGT ACAAGCTATA CCATCCATCT TCAATGTACT GCTAGTGTGC 
TTGATCTTTT GGTTGATTTT TGCTATAATG GGTGTACAAT TATTTGCTGG TAAATACTTT 
AAGTGCGTGG ACGCAAATAA GACGAGTCTG AGCTACGAAA TTATTCCCGA CTACAACGCT 
TGTATTGCCG AAAATTACAC GTGGGAGAAT TCCAGAATGA ATTTTGATCA CGTGGGTAAA 
GCCTACCTTT GCTTGTTTCA AGTCGCCACG TTTAAGGGCT GGATACAAAT CATGAACGAT 
GCGATAGATT CCAGAGAGAT TAATAAACAA CCAATAAGAG AGACGAACAT TTACATGTAT 
TTATATTTCG TCTTCTTTAT TATTTTTGGA TCGTTCTTTA CTTTGAATCT GTTTATTGGG 
GTGATCATTG ATAACTTTAA TGCACAAAAG AAGAAAGCCG GCGGTTCGTT GGAAATGTTC 
ATGACTGAGG ATCAGAAAAA GTACTACAAT GCGATGAAGA AAATGGGTTC GAAGAAGCCT 
ATGAAAGCGA TCCCGAGACC GAGGTGGAAG CCTCAAGCTA TCGTGTTTGA GATAGTAACA 
AATAAGAAAT TTGACATGAT AATCATGTTG TTCATTGGCT TGAACATGTT GACGATGACG 
ATGGACCATT ACCAGCAGAA AGAGACGTTT ACGCAAGTGC TAGACTACCT CAACATGATT 
TTCATAGTTA TATTTAGTAC CGAGTGCCTC ATGAAGATGT TTGCGTTGCG ATACCATTAC 
TTCACGGAAC CGTGGAATCT CTTCGATTTG GTAGTTGTGA TTTTGTCAAT CTTGGGTTTA 
GTTTTGAGTG ATATCATCGA AAAATATTTC GTATCACCAA CACTGTTGAG AGTGGTGCGT 
GTGGCAAAAG TCGGGAGGGT ACTCCGTTTG GTGAAAGGAG CAAAAGGCAT CCGCACACTG 
CTTTTCGCAT TAGCCATGTC ACTGCCTGCA CTATTCAACA TCTGTTTACT GTTATTCTTG 
GTGATGTTCA TCTTTGCCAT CTTCGGGATG TCCTTCTTCA TGCACGTCAA AGACAAGAGC 
GGATTGGATG ACGTCTACAA CTTCAAAACT TTTGCCCAGT CCATGATTCT CCTATTTCAG 
ATGTCCACTT CGGCCGGGTG GGACGGCGTT TTGGACGGGA TTATAAACGA AGAGGACTGC 
AAGTTGCCGA ACAATGAGAT AGGAGAGACG GGGAACTGCG GGAACTCAAC AATAGGCATC 
GCCTTTCTAC TTAGCTACCT CGTGATCTCT TTCCTGATCG TCATCAACAT GTACATCGCC 
GTGATCTTGG AGAACTACTC CCAGGCCACG GAAGACGTGC AGGAGGGCTT GACCGACGAC 
GACTACGACA TGTACTACGA GATCTGGCAG CAGTTCGACC CTGACGGCAC CCAGTACATC 
CGCTACGACC AGCTGTCGGA CTTCCTGGAT GTGCTGGAGC CGCCTTTGCA AATCCACAAA 
CCCAACAAAT ACAAGATAGT TTCCATGGAC ATCACCATAT GCAAGGGTGA TCTCATGTTT 
TGTGTAGATA TTTTAGATGC GCTAACTAAA GACTTCTTCG CCCGCAAGGG CAATCCGATT 
GAAGAGACCG CCGAACTCAG TGAAGTGAAC ACTCACCCGA ACGAGCCGGG CTACGAACCA 
GTGAGCTCCA CGCTGTGGAG GCAGCGCGAG GAGTACTGTG CGCGCCTGAT CCAGAACGCG 
TGGCGCAAGC ACAAGCAGCA GCGCAGCAGC GGCGTGCCCG AGCCGGCAGG CGAGGGGGAG 
CTGGACAAAG ACAGTGAGCT GGAGGCGCAC CAAACGGCCG TTTTGGTGGA GAACGACGGC 
TTTGCGACCA AAAACGGGCA CAAAGTGGTG TTGCACAGTC GCACGCCCAG CGTCAGCTCC 
AGGTCTGCGG ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015981938.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837424.1 Tribolium castaneum paralytic A (Para), transcript variant X3, mRNA.

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CloneID	OTg16167
Clone ID Related Accession (Same CDS sequence)	XM_015981938.1
Accession Version	XM_015981938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGCAAGTCTC AGTCTTCCGG GATCTCCGTT CAACCTTCGC 
AGAGGGTCCA GAGGCAGCCA CCAGTTCACC ATCCGGAACA ACAGGCACAT GGTGGGCGGC 
CCCTGCGACC GCAAACCTTT GGTTCTCTCG ACCTATCTCG ATGCTCAAGA ACATCTTCCC 
TACGCCGACG ACTCCAACGC TGTCACTCCC ATGAGCGAGG AGAACGGCGC CATGGTGTTC 
CCCATGTACT ACGCCAATCT GGGGTCACGT CACTCATCGT ACAACTCCCA CGCCGCGCGG 
ATGTCCTACA CCTCGCACGG GGACCTCCTT GGAGGCCTCG TAGGCAGTGG GAAAAACATG 
ACGAAGGAGA GCCAACTCCG GTGCCGTTCC ATGAGGAACG CACCTCCGGC CACAACAAAC 
TCGTTCTTTG AAATCACACA CAAAGCACAC CGAGGTGATT ATGATGGTCC CACGGGTCAG 
CTTTGTGAAT CGAAATTAAA ACAACTGGAT AGTTCCTTTA TTGACAACAA TCAGAGGCAA 
ACGGTTGTAG ATATGAAAGA CGTAAAAGTC CTCAACGATA TTATTGAGCA AGCGGCTGCC 
AGCGGCGGGA GTGAGCATGG AGTTTCCGTT TATATTTTCT CAGCGACTAA TAACGACGAG 
AATGACGAAG AAGAGGAGGA GCCCACGGTC GGCGAACGCA TGCTGGCCTA CACCCTCAAA 
ATAATCGACA TTTTCTGCGT CTGGGACTGT TGCCACTGCT GGGTCGTCAT CCAGAAGTTC 
GTCAGTTTGA TAGTGTTTGA CCCGTTCGTC GAGCTGTTCA TCACGCTCTG CATCGTCGTC 
AACACCCTGT TCATGGCGCT CGACCACCAC GACATGGACA AAGACCTGGA AAAGGCCCTA 
AAGAGCGGCA ACTACTTCTT CACCGCCACT TTCATGATCG AAGCTACGAT GAAGTTGATC 
GCGATGAGTC CGAAGTTCTA CTTTCAAGAA GGCTGGAATA TCTTTGATTT CATAATAGTG 
GCGTTGTCGT TGCTGGAACT TGGGCTTGAA GGGGTCCAGG GGTTGTCCGT TTTGAGATCA 
TTCCGACTGT TGAGGGTTTT TAAACTGGCA AAGTCGTGGC CAACCTTGAA CTTACTCATC 
TCCATTATGG GCAGGACGAT GGGGGCTTTG GGGAATCTAA CGTTTGTCTT GTGCATCATC 
ATATTCATAT TTGCCGTAAT GGGGATGCAA CTATTTGGGA AAAACTATAC AGATAACGTT 
GATAGATTTC CCGACCACGA ACTTCCAAGG TGGAACTTTA CCGATTTTAT GCATTCCTTT 
ATGATAGTGT TTAGAGTACT GTGTGGAGAG TGGATAGAAT CTATGTGGGA TTGCATGTTA 
GTAGGTGATG TGTCTTGTAT TCCATTCTTC CTAGCAACTG TGGTAATTGG TAATCTTGTG 
GTACTGAACC TCTTCTTGGC TCTGCTTTTG AGCAACTTTG GCTCTTCAAA CCTCTCAGCA 
CCGACTGCCG ACAACGACAC TAATAAAATA GCAGAGGCCT TTGAGAGAAT AGCGCGTTTT 
ATACGGTGGA TAAAGGGTAG TGTTTTAGAC ATGGTAAAAA TCGTCCGATT TAAACTAACT 
AATCAGATAT CAGACCAGCC CTCAGACGAA CGAGAGTGTG CGTTAGAAAT ATCAGAAGAC 
GAAATATTGG CTAACGGTTT AATGAAGAAA AACCCTAAAG ACCGGGTTGA AGTAACGATA 
GGCATAGGGA CCGGTATGGA CCTTACAGTT CATGGTGATT CGAAAGCGAA TTTGAAACGA 
GACAAGAACA GCTTGAGTAA AAGCAAAACT ATCGGAAATT CAATTTTAGA CCACAGTGAT 
TTTATTAGTC ACTTGGACGA CGACGAAATT AGTAATAATT CATACGGGAG TCACACTTAT 
CGCTTTAAGG ACGAGAGTCA CAAAGGCAGT GCCGACGTTC TTGAAGATAA CGAAGAGCAG 
GAAGAGAAGC GCGATGCTAG CAAAGAAGAG CTAGGTATCG CCGAAGACAC GGAAGAAGAA 
GGCTGCGAAT GTAAGGAAGT CCTGAATGAA GATTTGGTTG ACGCAAACAC TGAAGACATC 
ATCGACGAAT ATTCCGCCGA CTGCTGTCCC GAGTCGTGTT ACAAAAAGTT CCCGTTCCTG 
GCCGGCGACG AAGACTCGCC CTTTTGGCAA GGCTGGGCGA ACTTGCGCTA CAAAACGTTC 
CGGCTGATAG AAAACAAATA TTTCGAAACG GCGGTCATCA CGATGATTTT GCTGAGCAGT 
CTGGCCCTGG CTTTGGAGGA TGTGCATCTT TCGAAACGGC CGATCTTGCA AGACATTTTA 
TACTACATGG ACCGCATTTT TACAGTGATT TTCTTTTTCG AAATGTTGAT AAAGTGGCTG 
GCTTTAGGTT TCCAAAAGTA CTTTACGAAC GCATGGTGCT GGCTAGATTT TATTATCGTA 
ATGGTGTCCT TAATTAATTT TATTGCCTCT CTTTGTGGCG CTGGTGGTAT TCAAGCGTTT 
AAAACCATGA GAACACTCCG AGCTCTGAGG CCGCTCCGTG CCATGTCGCG GATGCAAGGG 
ATGAGGGTTG TGGTGAACGC ACTCGTACAA GCTATACCAT CCATCTTCAA TGTACTGCTA 
GTGTGCTTGA TCTTTTGGTT GATTTTTGCT ATAATGGGTG TACAATTATT TGCTGGTAAA 
TACTTTAAGT GCGTGGACGC AAATAAGACG AGTCTGAGCT ACGAAATTAT TCCCGACTAC 
AACGCTTGTA TTGCCGAAAA TTACACGTGG GAGAATTCCA GAATGAATTT TGATCACGTG 
GGTAAAGCCT ACCTTTGCTT GTTTCAAGTC GCCACGTTTA AGGGCTGGAT ACAAATCATG 
AACGATGCGA TAGATTCCAG AGAGATTAAT AAACAACCAA TAAGAGAGAC GAACATTTAC 
ATGTATTTAT ATTTCGTCTT CTTTATTATT TTTGGATCGT TCTTTACTTT GAATCTGTTT 
ATTGGGGTGA TCATTGATAA CTTTAATGCA CAAAAGAAGA AAGCCGGCGG TTCGTTGGAA 
ATGTTCATGA CTGAGGATCA GAAAAAGTAC TACAATGCGA TGAAGAAAAT GGGTTCGAAG 
AAGCCTATGA AAGCGATCCC GAGACCGAGG TGGAAGCCTC AAGCTATCGT GTTTGAGATA 
GTAACAAATA AGAAATTTGA CATGATAATC ATGTTGTTCA TTGGCTTGAA CATGTTGACG 
ATGACGATGG ACCATTACCA GCAGAAAGAG ACGTTTACGC AAGTGCTAGA CTACCTCAAC 
ATGATTTTCA TAGTTATATT TAGTACCGAG TGCCTCATGA AGATGTTTGC GTTGCGATAC 
CATTACTTCA CGGAACCGTG GAATCTCTTC GATTTGGTAG TTGTGATTTT GTCAATCTTG 
GGTTTAGTTT TGAGTGATAT CATCGAAAAA TATTTCGTAT CACCAACACT GTTGAGAGTG 
GTGCGTGTGG CAAAAGTCGG GAGGGTACTC CGTTTGGTGA AAGGAGCAAA AGGCATCCGC 
ACACTGCTTT TCGCATTAGC CATGTCACTG CCTGCACTAT TCAACATCTG TTTACTGTTA 
TTCTTGGTGA TGTTCATCTT TGCCATCTTC GGGATGTCCT TCTTCATGCA CGTCAAAGAC 
AAGAGCGGAT TGGATGACGT CTACAACTTC AAAACTTTTG CCCAGTCCAT GATTCTCCTA 
TTTCAGATGT CCACTTCGGC CGGGTGGGAC GGCGTTTTGG ACGGGATTAT AAACGAAGAG 
GACTGCAAGT TGCCGAACAA TGAGATAGGA GAGACGGGGA ACTGCGGGAA CTCAACAATA 
GGCATCGCCT TTCTACTTAG CTACCTCGTG ATCTCTTTCC TGATCGTCAT CAACATGTAC 
ATCGCCGTGA TCTTGGAGAA CTACTCCCAG GCCACGGAAG ACGTGCAGGA GGGCTTGACC 
GACGACGACT ACGACATGTA CTACGAGATC TGGCAGCAGT TCGACCCTGA CGGCACCCAG 
TACATCCGCT ACGACCAGCT GTCGGACTTC CTGGATGTGC TGGAGCCGCC TTTGCAAATC 
CACAAACCCA ACAAATACAA GATAGTTTCC ATGGACATCA CCATATGCAA GGGTGATCTC 
ATGTTTTGTG TAGATATTTT AGATGCGCTA ACTAAAGACT TCTTCGCCCG CAAGGGCAAT 
CCGATTGAAG AGACCGCCGA ACTCAGTGAA GTGAACACTC ACCCGAACGA GCCGGGCTAC 
GAACCAGTGA GCTCCACGCT GTGGAGGCAG CGCGAGGAGT ACTGTGCGCG CCTGATCCAG 
AACGCGTGGC GCAAGCACAA GCAGCAGCGC AGCAGCGGCG TGCCCGAGCC GGCAGGCGAG 
GGGGAGCTGG ACAAAGACAG TGAGCTGGAG GCGCACCAAA CGGCCGTTTT GGTGGAGAAC 
GACGGCTTTG CGACCAAAAA CGGGCACAAA GTGGTGTTGC ACAGTCGCAC GCCCAGCGTC 
AGCTCCAGGT CTGCGGACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837424.1
CDS	2125..8208
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGETGFGRRKKKKEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015981938.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015981938.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGCAAGTCTC AGTCTTCCGG GATCTCCGTT CAACCTTCGC 
AGAGGGTCCA GAGGCAGCCA CCAGTTCACC ATCCGGAACA ACAGGCACAT GGTGGGCGGC 
CCCTGCGACC GCAAACCTTT GGTTCTCTCG ACCTATCTCG ATGCTCAAGA ACATCTTCCC 
TACGCCGACG ACTCCAACGC TGTCACTCCC ATGAGCGAGG AGAACGGCGC CATGGTGTTC 
CCCATGTACT ACGCCAATCT GGGGTCACGT CACTCATCGT ACAACTCCCA CGCCGCGCGG 
ATGTCCTACA CCTCGCACGG GGACCTCCTT GGAGGCCTCG TAGGCAGTGG GAAAAACATG 
ACGAAGGAGA GCCAACTCCG GTGCCGTTCC ATGAGGAACG CACCTCCGGC CACAACAAAC 
TCGTTCTTTG AAATCACACA CAAAGCACAC CGAGGTGATT ATGATGGTCC CACGGGTCAG 
CTTTGTGAAT CGAAATTAAA ACAACTGGAT AGTTCCTTTA TTGACAACAA TCAGAGGCAA 
ACGGTTGTAG ATATGAAAGA CGTAAAAGTC CTCAACGATA TTATTGAGCA AGCGGCTGCC 
AGCGGCGGGA GTGAGCATGG AGTTTCCGTT TATATTTTCT CAGCGACTAA TAACGACGAG 
AATGACGAAG AAGAGGAGGA GCCCACGGTC GGCGAACGCA TGCTGGCCTA CACCCTCAAA 
ATAATCGACA TTTTCTGCGT CTGGGACTGT TGCCACTGCT GGGTCGTCAT CCAGAAGTTC 
GTCAGTTTGA TAGTGTTTGA CCCGTTCGTC GAGCTGTTCA TCACGCTCTG CATCGTCGTC 
AACACCCTGT TCATGGCGCT CGACCACCAC GACATGGACA AAGACCTGGA AAAGGCCCTA 
AAGAGCGGCA ACTACTTCTT CACCGCCACT TTCATGATCG AAGCTACGAT GAAGTTGATC 
GCGATGAGTC CGAAGTTCTA CTTTCAAGAA GGCTGGAATA TCTTTGATTT CATAATAGTG 
GCGTTGTCGT TGCTGGAACT TGGGCTTGAA GGGGTCCAGG GGTTGTCCGT TTTGAGATCA 
TTCCGACTGT TGAGGGTTTT TAAACTGGCA AAGTCGTGGC CAACCTTGAA CTTACTCATC 
TCCATTATGG GCAGGACGAT GGGGGCTTTG GGGAATCTAA CGTTTGTCTT GTGCATCATC 
ATATTCATAT TTGCCGTAAT GGGGATGCAA CTATTTGGGA AAAACTATAC AGATAACGTT 
GATAGATTTC CCGACCACGA ACTTCCAAGG TGGAACTTTA CCGATTTTAT GCATTCCTTT 
ATGATAGTGT TTAGAGTACT GTGTGGAGAG TGGATAGAAT CTATGTGGGA TTGCATGTTA 
GTAGGTGATG TGTCTTGTAT TCCATTCTTC CTAGCAACTG TGGTAATTGG TAATCTTGTG 
GTACTGAACC TCTTCTTGGC TCTGCTTTTG AGCAACTTTG GCTCTTCAAA CCTCTCAGCA 
CCGACTGCCG ACAACGACAC TAATAAAATA GCAGAGGCCT TTGAGAGAAT AGCGCGTTTT 
ATACGGTGGA TAAAGGGTAG TGTTTTAGAC ATGGTAAAAA TCGTCCGATT TAAACTAACT 
AATCAGATAT CAGACCAGCC CTCAGACGAA CGAGAGTGTG CGTTAGAAAT ATCAGAAGAC 
GAAATATTGG CTAACGGTTT AATGAAGAAA AACCCTAAAG ACCGGGTTGA AGTAACGATA 
GGCATAGGGA CCGGTATGGA CCTTACAGTT CATGGTGATT CGAAAGCGAA TTTGAAACGA 
GACAAGAACA GCTTGAGTAA AAGCAAAACT ATCGGAAATT CAATTTTAGA CCACAGTGAT 
TTTATTAGTC ACTTGGACGA CGACGAAATT AGTAATAATT CATACGGGAG TCACACTTAT 
CGCTTTAAGG ACGAGAGTCA CAAAGGCAGT GCCGACGTTC TTGAAGATAA CGAAGAGCAG 
GAAGAGAAGC GCGATGCTAG CAAAGAAGAG CTAGGTATCG CCGAAGACAC GGAAGAAGAA 
GGCTGCGAAT GTAAGGAAGT CCTGAATGAA GATTTGGTTG ACGCAAACAC TGAAGACATC 
ATCGACGAAT ATTCCGCCGA CTGCTGTCCC GAGTCGTGTT ACAAAAAGTT CCCGTTCCTG 
GCCGGCGACG AAGACTCGCC CTTTTGGCAA GGCTGGGCGA ACTTGCGCTA CAAAACGTTC 
CGGCTGATAG AAAACAAATA TTTCGAAACG GCGGTCATCA CGATGATTTT GCTGAGCAGT 
CTGGCCCTGG CTTTGGAGGA TGTGCATCTT TCGAAACGGC CGATCTTGCA AGACATTTTA 
TACTACATGG ACCGCATTTT TACAGTGATT TTCTTTTTCG AAATGTTGAT AAAGTGGCTG 
GCTTTAGGTT TCCAAAAGTA CTTTACGAAC GCATGGTGCT GGCTAGATTT TATTATCGTA 
ATGGTGTCCT TAATTAATTT TATTGCCTCT CTTTGTGGCG CTGGTGGTAT TCAAGCGTTT 
AAAACCATGA GAACACTCCG AGCTCTGAGG CCGCTCCGTG CCATGTCGCG GATGCAAGGG 
ATGAGGGTTG TGGTGAACGC ACTCGTACAA GCTATACCAT CCATCTTCAA TGTACTGCTA 
GTGTGCTTGA TCTTTTGGTT GATTTTTGCT ATAATGGGTG TACAATTATT TGCTGGTAAA 
TACTTTAAGT GCGTGGACGC AAATAAGACG AGTCTGAGCT ACGAAATTAT TCCCGACTAC 
AACGCTTGTA TTGCCGAAAA TTACACGTGG GAGAATTCCA GAATGAATTT TGATCACGTG 
GGTAAAGCCT ACCTTTGCTT GTTTCAAGTC GCCACGTTTA AGGGCTGGAT ACAAATCATG 
AACGATGCGA TAGATTCCAG AGAGATTAAT AAACAACCAA TAAGAGAGAC GAACATTTAC 
ATGTATTTAT ATTTCGTCTT CTTTATTATT TTTGGATCGT TCTTTACTTT GAATCTGTTT 
ATTGGGGTGA TCATTGATAA CTTTAATGCA CAAAAGAAGA AAGCCGGCGG TTCGTTGGAA 
ATGTTCATGA CTGAGGATCA GAAAAAGTAC TACAATGCGA TGAAGAAAAT GGGTTCGAAG 
AAGCCTATGA AAGCGATCCC GAGACCGAGG TGGAAGCCTC AAGCTATCGT GTTTGAGATA 
GTAACAAATA AGAAATTTGA CATGATAATC ATGTTGTTCA TTGGCTTGAA CATGTTGACG 
ATGACGATGG ACCATTACCA GCAGAAAGAG ACGTTTACGC AAGTGCTAGA CTACCTCAAC 
ATGATTTTCA TAGTTATATT TAGTACCGAG TGCCTCATGA AGATGTTTGC GTTGCGATAC 
CATTACTTCA CGGAACCGTG GAATCTCTTC GATTTGGTAG TTGTGATTTT GTCAATCTTG 
GGTTTAGTTT TGAGTGATAT CATCGAAAAA TATTTCGTAT CACCAACACT GTTGAGAGTG 
GTGCGTGTGG CAAAAGTCGG GAGGGTACTC CGTTTGGTGA AAGGAGCAAA AGGCATCCGC 
ACACTGCTTT TCGCATTAGC CATGTCACTG CCTGCACTAT TCAACATCTG TTTACTGTTA 
TTCTTGGTGA TGTTCATCTT TGCCATCTTC GGGATGTCCT TCTTCATGCA CGTCAAAGAC 
AAGAGCGGAT TGGATGACGT CTACAACTTC AAAACTTTTG CCCAGTCCAT GATTCTCCTA 
TTTCAGATGT CCACTTCGGC CGGGTGGGAC GGCGTTTTGG ACGGGATTAT AAACGAAGAG 
GACTGCAAGT TGCCGAACAA TGAGATAGGA GAGACGGGGA ACTGCGGGAA CTCAACAATA 
GGCATCGCCT TTCTACTTAG CTACCTCGTG ATCTCTTTCC TGATCGTCAT CAACATGTAC 
ATCGCCGTGA TCTTGGAGAA CTACTCCCAG GCCACGGAAG ACGTGCAGGA GGGCTTGACC 
GACGACGACT ACGACATGTA CTACGAGATC TGGCAGCAGT TCGACCCTGA CGGCACCCAG 
TACATCCGCT ACGACCAGCT GTCGGACTTC CTGGATGTGC TGGAGCCGCC TTTGCAAATC 
CACAAACCCA ACAAATACAA GATAGTTTCC ATGGACATCA CCATATGCAA GGGTGATCTC 
ATGTTTTGTG TAGATATTTT AGATGCGCTA ACTAAAGACT TCTTCGCCCG CAAGGGCAAT 
CCGATTGAAG AGACCGCCGA ACTCAGTGAA GTGAACACTC ACCCGAACGA GCCGGGCTAC 
GAACCAGTGA GCTCCACGCT GTGGAGGCAG CGCGAGGAGT ACTGTGCGCG CCTGATCCAG 
AACGCGTGGC GCAAGCACAA GCAGCAGCGC AGCAGCGGCG TGCCCGAGCC GGCAGGCGAG 
GGGGAGCTGG ACAAAGACAG TGAGCTGGAG GCGCACCAAA CGGCCGTTTT GGTGGAGAAC 
GACGGCTTTG CGACCAAAAA CGGGCACAAA GTGGTGTTGC ACAGTCGCAC GCCCAGCGTC 
AGCTCCAGGT CTGCGGACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015981971.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837457.1 Tribolium castaneum paralytic A (Para), transcript variant X4, mRNA.

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CloneID	OTg16168
Clone ID Related Accession (Same CDS sequence)	XM_015981971.1
Accession Version	XM_015981971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGC 
CCCCAACCTG ACCCAACTCT GGAGCAAGGG TTACCCATAC CGGTCCGTCT TCAGGGTGGG 
TTTCCCCCGG AACTGGCCAG CACACCACTT GAAGACATCG ACCCTTTTTA CAGCAACCAA 
AGAACTTTTG TAGTAGTTAG TAAAGGCAAG GACATATTCC GATTTAGTGC AACCAACGCT 
CTGTGGATAC TCGATCCTTT CAATCCAATC CGTCGAGTAG CCATTTATAT CTTAGTGCAT 
CCTTTATTTT CTCTTTTCAT CATCACCACC ATCCTCGTCA ATTGTATCCT CATGATTATG 
CCCACCACAC CCACCGTAGA ATCAACTGAA GTAATTTTCA CTGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC CAGAGGTTTT ATTCTTCAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAATT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTTACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACGAT GTTTTCGCTG TCAGTGTTCG CACTGATGGG TTTACAGATT 
TACATGGGGG TTCTGACGCA AAAATGTATC AAAAATTTCC CCATGGACGG CTCCTGGGGA 
AATCTCACCG CTGAAAACTG GGAAAGATTT GTAAGCAACG AAACGAATTG GTATTGGGAC 
GAAGAAAAGG GGGAGCGGCC TTTATGCGGA AACTCCTCCG GCGCCGGCAC ATGTAAACCT 
GGTTATACAT GTTTACAAGG CTATGGTGAA AATCCAAATT ATGGGTACAC AAGTTTTGAT 
ACATTTGGCT GGGCCTTCCT TTCTGCCTTT AGACTAATGA CTCAGGATTA TTGGGAAAAT 
TTATATCAAT TGGTTTTGAG ATCTGCAGGA CCTTGGCACA TGTTGTTTTT TATCGTGATC 
ATCTTCTTGG GCTCGTTTTA TTTGGTCAAC TTAATTTTGG CCATCGTCGC TATGTCTTAT 
GATGAATTAC AAAAGAAGGC TCAGGATGAA GAAGAGGCCG AAGCCGAAGC CATCAGAGAA 
GCTGAAGAGA AGGCGAAGGA GAAGCAGGAC CGGGCGGACG CGCGAGCCTT GGCGGCGGTG 
GAGGCAGCTG CGGCCGCGGA AGCGGCTGCC AGTGGCGGCG GCCACGAGAT CGTGAAGTCT 
TCGTCCGACT ATTCCTGTCA TAGCTATGAA AGCTTCGTAG GACAAGCGAG CGGCCAGGAT 
GACAACAACA AGGAAAAAAT AAGCATACGA TCTGAAGGAT TAGATTCCGT GAGTGAACAC 
AGAAGAGTTC CTAATCTCAG CAAGATACGG AAAGTCAGCG CTGCAAGTCT CAGTCTTCCG 
GGATCTCCGT TCAACCTTCG CAGAGGGTCC AGAGGCAGCC ACCAGTTCAC CATCCGGAAC 
AACAGGCACA TGGTGGGCGG CCCCTGCGAC CGCAAACCTT TGGTTCTCTC GACCTATCTC 
GATGCTCAAG AACATCTTCC CTACGCCGAC GACTCCAACG CTGTCACTCC CATGAGCGAG 
GAGAACGGCG CCATGGTGTT CCCCATGTAC TACGCCAATC TGGGGTCACG TCACTCATCG 
TACAACTCCC ACGCCGCGCG GATGTCCTAC ACCTCGCACG GGGACCTCCT TGGAGGCCTC 
GTAGGCAGTG GGAAAAACAT GACGAAGGAG AGCCAACTCC GGTGCCGTTC CATGAGGAAC 
GCACCTCCGG CCACAACAAA CTCGTTCTTT GAAATCACAC ACAAAGCACA CCGAGGTGAT 
TATGATGGTC CCACGGGTCA GCTTTGTGAA TCGAAATTAA AACAACTGGA TAGTTCCTTT 
ATTGACAACA ATCAGAGGCA AACGGTTGTA GATATGAAAG ACGTAAAAGT CCTCAACGAT 
ATTATTGAGC AAGCGGCTGC CAGCGGCGGG AGTGAGCATG GAGTTTCCGT TTATATTTTC 
TCAGCGACTA ATAACGACGA GAATGACGAA GAAGAGGAGG AGCCCACGGT CGGCGAACGC 
ATGCTGGCCT ACACCCTCAA AATAATCGAC ATTTTCTGCG TCTGGGACTG TTGCCACTGC 
TGGGTCGTCA TCCAGAAGTT CGTCAGTTTG ATAGTGTTTG ACCCGTTCGT CGAGCTGTTC 
ATCACGCTCT GCATCGTCGT CAACACCCTG TTCATGGCGC TCGACCACCA CGACATGGAC 
AAAGACCTGG AAAAGGCCCT AAAGAGCGGC AACTACTTCT TCACCGCCAC TTTCATGATC 
GAAGCTACGA TGAAGTTGAT CGCGATGAGT CCGAAGTTCT ACTTTCAAGA AGGCTGGAAT 
ATCTTTGATT TCATAATAGT GGCGTTGTCG TTGCTGGAAC TTGGGCTTGA AGGGGTCCAG 
GGGTTGTCCG TTTTGAGATC ATTCCGACTG TTGAGGGTTT TTAAACTGGC AAAGTCGTGG 
CCAACCTTGA ACTTACTCAT CTCCATTATG GGCAGGACGA TGGGGGCTTT GGGGAATCTA 
ACGTTTGTCT TGTGCATCAT CATATTCATA TTTGCCGTAA TGGGGATGCA ACTATTTGGG 
AAAAACTATA CAGATAACGT TGATAGATTT CCCGACCACG AACTTCCAAG GTGGAACTTT 
ACCGATTTTA TGCATTCCTT TATGATAGTG TTTAGAGTAC TGTGTGGAGA GTGGATAGAA 
TCTATGTGGG ATTGCATGTT AGTAGGTGAT GTGTCTTGTA TTCCATTCTT CCTAGCAACT 
GTGGTAATTG GTAATCTTGT GGTACTGAAC CTCTTCTTGG CTCTGCTTTT GAGCAACTTT 
GGCTCTTCAA ACCTCTCAGC ACCGACTGCC GACAACGACA CTAATAAAAT AGCAGAGGCC 
TTTGAGAGAA TAGCGCGTTT TATACGGTGG ATAAAGGGTA GTGTTTTAGA CATGGTAAAA 
ATCGTCCGAT TTAAACTAAC TAATCAGATA TCAGACCAGC CCTCAGGTAA AAAAAGAAAC 
TTATCTCTAG TTAGTCACTT GGTAATTTCA GACGAACGAG AGTGTGCGTT AGAAATATCA 
GAAGACGAAA TATTGGCTAA CGGTTTAATG AAGAAAAACC CTAAAGACCG GGTTGAAGTA 
ACGATAGGCA TAGGGACCGG TATGGACCTT ACAGTTCATG GTGATTCGAA AGCGAATTTG 
AAACGAGACA AGAACAGCTT GAGTAAAAGC AAAACTATCG GAAATTCAAT TTTAGACCAC 
AGTGATTTTA TTAGTCACTT GGACGACGAC GAAATTAGTA ATAATTCATA CGGGAGTCAC 
ACTTATCGCT TTAAGGACGA GAGTCACAAA GGCAGTGCCG ACGTTCTTGA AGATAACGAA 
GAGCAGGAAG AGAAGCGCGA TGCTAGCAAA GAAGAGCTAG GTATCGCCGA AGACACGGAA 
GAAGAAGGCT GCGAATGTAA GGAAGTCCTG AATGAAGATT TGGTTGACGC AAACACTGAA 
GACATCATCG ACGAATATTC CGCCGACTGC TGTCCCGAGT CGTGTTACAA AAAGTTCCCG 
TTCCTGGCCG GCGACGAAGA CTCGCCCTTT TGGCAAGGCT GGGCGAACTT GCGCTACAAA 
ACGTTCCGGC TGATAGAAAA CAAATATTTC GAAACGGCGG TCATCACGAT GATTTTGCTG 
AGCAGTCTGG CCCTGGCTTT GGAGGATGTG CATCTTTCGA AACGGCCGAT CTTGCAAGAC 
ATTTTATACT ACATGGACCG CATTTTTACA GTGATTTTCT TTTTCGAAAT GTTGATAAAG 
TGGCTGGCTT TAGGTTTCCA AAAGTACTTT ACGAACGCAT GGTGCTGGCT AGATTTTATT 
ATCGTAATGG TGTCCTTAAT TAATTTTATT GCCTCTCTTT GTGGCGCTGG TGGTATTCAA 
GCGTTTAAAA CCATGAGAAC ACTCCGAGCT CTGAGGCCGC TCCGTGCCAT GTCGCGGATG 
CAAGGGATGA GGGTTGTGGT GAACGCACTC GTACAAGCTA TACCATCCAT CTTCAATGTA 
CTGCTAGTGT GCTTGATCTT TTGGTTGATT TTTGCTATAA TGGGTGTACA ATTATTTGCT 
GGTAAATACT TTAAGTGCGT GGACGCAAAT AAGACGAGTC TGAGCTACGA AATTATTCCC 
GACTACAACG CTTGTATTGC CGAAAATTAC ACGTGGGAGA ATTCCAGAAT GAATTTTGAT 
CACGTGGGTA AAGCCTACCT TTGCTTGTTT CAAGTCGCCA CGTTTAAGGG CTGGATACAA 
ATCATGAACG ATGCGATAGA TTCCAGAGAG ATTAATAAAC AACCAATAAG AGAGACGAAC 
ATTTACATGT ATTTATATTT CGTCTTCTTT ATTATTTTTG GATCGTTCTT TACTTTGAAT 
CTGTTTATTG GGGTGATCAT TGATAACTTT AATGCACAAA AGAAGAAAGC CGGCGGTTCG 
TTGGAAATGT TCATGACTGA GGATCAGAAA AAGTACTACA ATGCGATGAA GAAAATGGGT 
TCGAAGAAGC CTATGAAAGC GATCCCGAGA CCGAGGTGGA AGCCTCAAGC TATCGTGTTT 
GAGATAGTAA CAAATAAGAA ATTTGACATG ATAATCATGT TGTTCATTGG CTTGAACATG 
TTGACGATGA CGATGGACCA TTACCAGCAG AAAGAGACGT TTACGCAAGT GCTAGACTAC 
CTCAACATGA TTTTCATAGT TATATTTAGT ACCGAGTGCC TCATGAAGAT GTTTGCGTTG 
CGATACCATT ACTTCACGGA ACCGTGGAAT CTCTTCGATT TGGTAGTTGT GATTTTGTCA 
ATCTTGGGTT TAGTTTTGAG TGATATCATC GAAAAATATT TCGTATCACC AACACTGTTG 
AGAGTGGTGC GTGTGGCAAA AGTCGGGAGG GTACTCCGTT TGGTGAAAGG AGCAAAAGGC 
ATCCGCACAC TGCTTTTCGC ATTAGCCATG TCACTGCCTG CACTATTCAA CATCTGTTTA 
CTGTTATTCT TGGTGATGTT CATCTTTGCC ATCTTCGGGA TGTCCTTCTT CATGCACGTC 
AAAGACAAGA GCGGATTGGA TGACGTCTAC AACTTCAAAA CTTTTGCCCA GTCCATGATT 
CTCCTATTTC AGATGTCCAC TTCGGCCGGG TGGGACGGCG TTTTGGACGG GATTATAAAC 
GAAGAGGACT GCAAGTTGCC GAACAATGAG ATAGGAGAGA CGGGGAACTG CGGGAACTCA 
ACAATAGGCA TCGCCTTTCT ACTTAGCTAC CTCGTGATCT CTTTCCTGAT CGTCATCAAC 
ATGTACATCG CCGTGATCTT GGAGAACTAC TCCCAGGCCA CGGAAGACGT GCAGGAGGGC 
TTGACCGACG ACGACTACGA CATGTACTAC GAGATCTGGC AGCAGTTCGA CCCTGACGGC 
ACCCAGTACA TCCGCTACGA CCAGCTGTCG GACTTCCTGG ATGTGCTGGA GCCGCCTTTG 
CAAATCCACA AACCCAACAA ATACAAGATA GTTTCCATGG ACATCACCAT ATGCAAGGGT 
GATCTCATGT TTTGTGTAGA TATTTTAGAT GCGCTAACTA AAGACTTCTT CGCCCGCAAG 
GGCAATCCGA TTGAAGAGAC CGCCGAACTC AGTGAAGTGA ACACTCACCC GAACGAGCCG 
GGCTACGAAC CAGTGAGCTC CACGCTGTGG AGGCAGCGCG AGGAGTACTG TGCGCGCCTG 
ATCCAGAACG CGTGGCGCAA GCACAAGCAG CAGCGCAGCA GCGGCGTGCC CGAGCCGGCA 
GGCGAGGGGG AGCTGGACAA AGACAGTGAG CTGGAGGCGC ACCAAACGGC CGTTTTGGTG 
GAGAACGACG GCTTTGCGAC CAAAAACGGG CACAAAGTGG TGTTGCACAG TCGCACGCCC 
AGCGTCAGCT CCAGGTCTGC GGACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837457.1
CDS	2126..8275
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSVSEHRRVPNLSKIRKVSAASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSGKKRNLSLVSHLVISDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015981971.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015981971.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGC 
CCCCAACCTG ACCCAACTCT GGAGCAAGGG TTACCCATAC CGGTCCGTCT TCAGGGTGGG 
TTTCCCCCGG AACTGGCCAG CACACCACTT GAAGACATCG ACCCTTTTTA CAGCAACCAA 
AGAACTTTTG TAGTAGTTAG TAAAGGCAAG GACATATTCC GATTTAGTGC AACCAACGCT 
CTGTGGATAC TCGATCCTTT CAATCCAATC CGTCGAGTAG CCATTTATAT CTTAGTGCAT 
CCTTTATTTT CTCTTTTCAT CATCACCACC ATCCTCGTCA ATTGTATCCT CATGATTATG 
CCCACCACAC CCACCGTAGA ATCAACTGAA GTAATTTTCA CTGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC CAGAGGTTTT ATTCTTCAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAATT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTTACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACGAT GTTTTCGCTG TCAGTGTTCG CACTGATGGG TTTACAGATT 
TACATGGGGG TTCTGACGCA AAAATGTATC AAAAATTTCC CCATGGACGG CTCCTGGGGA 
AATCTCACCG CTGAAAACTG GGAAAGATTT GTAAGCAACG AAACGAATTG GTATTGGGAC 
GAAGAAAAGG GGGAGCGGCC TTTATGCGGA AACTCCTCCG GCGCCGGCAC ATGTAAACCT 
GGTTATACAT GTTTACAAGG CTATGGTGAA AATCCAAATT ATGGGTACAC AAGTTTTGAT 
ACATTTGGCT GGGCCTTCCT TTCTGCCTTT AGACTAATGA CTCAGGATTA TTGGGAAAAT 
TTATATCAAT TGGTTTTGAG ATCTGCAGGA CCTTGGCACA TGTTGTTTTT TATCGTGATC 
ATCTTCTTGG GCTCGTTTTA TTTGGTCAAC TTAATTTTGG CCATCGTCGC TATGTCTTAT 
GATGAATTAC AAAAGAAGGC TCAGGATGAA GAAGAGGCCG AAGCCGAAGC CATCAGAGAA 
GCTGAAGAGA AGGCGAAGGA GAAGCAGGAC CGGGCGGACG CGCGAGCCTT GGCGGCGGTG 
GAGGCAGCTG CGGCCGCGGA AGCGGCTGCC AGTGGCGGCG GCCACGAGAT CGTGAAGTCT 
TCGTCCGACT ATTCCTGTCA TAGCTATGAA AGCTTCGTAG GACAAGCGAG CGGCCAGGAT 
GACAACAACA AGGAAAAAAT AAGCATACGA TCTGAAGGAT TAGATTCCGT GAGTGAACAC 
AGAAGAGTTC CTAATCTCAG CAAGATACGG AAAGTCAGCG CTGCAAGTCT CAGTCTTCCG 
GGATCTCCGT TCAACCTTCG CAGAGGGTCC AGAGGCAGCC ACCAGTTCAC CATCCGGAAC 
AACAGGCACA TGGTGGGCGG CCCCTGCGAC CGCAAACCTT TGGTTCTCTC GACCTATCTC 
GATGCTCAAG AACATCTTCC CTACGCCGAC GACTCCAACG CTGTCACTCC CATGAGCGAG 
GAGAACGGCG CCATGGTGTT CCCCATGTAC TACGCCAATC TGGGGTCACG TCACTCATCG 
TACAACTCCC ACGCCGCGCG GATGTCCTAC ACCTCGCACG GGGACCTCCT TGGAGGCCTC 
GTAGGCAGTG GGAAAAACAT GACGAAGGAG AGCCAACTCC GGTGCCGTTC CATGAGGAAC 
GCACCTCCGG CCACAACAAA CTCGTTCTTT GAAATCACAC ACAAAGCACA CCGAGGTGAT 
TATGATGGTC CCACGGGTCA GCTTTGTGAA TCGAAATTAA AACAACTGGA TAGTTCCTTT 
ATTGACAACA ATCAGAGGCA AACGGTTGTA GATATGAAAG ACGTAAAAGT CCTCAACGAT 
ATTATTGAGC AAGCGGCTGC CAGCGGCGGG AGTGAGCATG GAGTTTCCGT TTATATTTTC 
TCAGCGACTA ATAACGACGA GAATGACGAA GAAGAGGAGG AGCCCACGGT CGGCGAACGC 
ATGCTGGCCT ACACCCTCAA AATAATCGAC ATTTTCTGCG TCTGGGACTG TTGCCACTGC 
TGGGTCGTCA TCCAGAAGTT CGTCAGTTTG ATAGTGTTTG ACCCGTTCGT CGAGCTGTTC 
ATCACGCTCT GCATCGTCGT CAACACCCTG TTCATGGCGC TCGACCACCA CGACATGGAC 
AAAGACCTGG AAAAGGCCCT AAAGAGCGGC AACTACTTCT TCACCGCCAC TTTCATGATC 
GAAGCTACGA TGAAGTTGAT CGCGATGAGT CCGAAGTTCT ACTTTCAAGA AGGCTGGAAT 
ATCTTTGATT TCATAATAGT GGCGTTGTCG TTGCTGGAAC TTGGGCTTGA AGGGGTCCAG 
GGGTTGTCCG TTTTGAGATC ATTCCGACTG TTGAGGGTTT TTAAACTGGC AAAGTCGTGG 
CCAACCTTGA ACTTACTCAT CTCCATTATG GGCAGGACGA TGGGGGCTTT GGGGAATCTA 
ACGTTTGTCT TGTGCATCAT CATATTCATA TTTGCCGTAA TGGGGATGCA ACTATTTGGG 
AAAAACTATA CAGATAACGT TGATAGATTT CCCGACCACG AACTTCCAAG GTGGAACTTT 
ACCGATTTTA TGCATTCCTT TATGATAGTG TTTAGAGTAC TGTGTGGAGA GTGGATAGAA 
TCTATGTGGG ATTGCATGTT AGTAGGTGAT GTGTCTTGTA TTCCATTCTT CCTAGCAACT 
GTGGTAATTG GTAATCTTGT GGTACTGAAC CTCTTCTTGG CTCTGCTTTT GAGCAACTTT 
GGCTCTTCAA ACCTCTCAGC ACCGACTGCC GACAACGACA CTAATAAAAT AGCAGAGGCC 
TTTGAGAGAA TAGCGCGTTT TATACGGTGG ATAAAGGGTA GTGTTTTAGA CATGGTAAAA 
ATCGTCCGAT TTAAACTAAC TAATCAGATA TCAGACCAGC CCTCAGGTAA AAAAAGAAAC 
TTATCTCTAG TTAGTCACTT GGTAATTTCA GACGAACGAG AGTGTGCGTT AGAAATATCA 
GAAGACGAAA TATTGGCTAA CGGTTTAATG AAGAAAAACC CTAAAGACCG GGTTGAAGTA 
ACGATAGGCA TAGGGACCGG TATGGACCTT ACAGTTCATG GTGATTCGAA AGCGAATTTG 
AAACGAGACA AGAACAGCTT GAGTAAAAGC AAAACTATCG GAAATTCAAT TTTAGACCAC 
AGTGATTTTA TTAGTCACTT GGACGACGAC GAAATTAGTA ATAATTCATA CGGGAGTCAC 
ACTTATCGCT TTAAGGACGA GAGTCACAAA GGCAGTGCCG ACGTTCTTGA AGATAACGAA 
GAGCAGGAAG AGAAGCGCGA TGCTAGCAAA GAAGAGCTAG GTATCGCCGA AGACACGGAA 
GAAGAAGGCT GCGAATGTAA GGAAGTCCTG AATGAAGATT TGGTTGACGC AAACACTGAA 
GACATCATCG ACGAATATTC CGCCGACTGC TGTCCCGAGT CGTGTTACAA AAAGTTCCCG 
TTCCTGGCCG GCGACGAAGA CTCGCCCTTT TGGCAAGGCT GGGCGAACTT GCGCTACAAA 
ACGTTCCGGC TGATAGAAAA CAAATATTTC GAAACGGCGG TCATCACGAT GATTTTGCTG 
AGCAGTCTGG CCCTGGCTTT GGAGGATGTG CATCTTTCGA AACGGCCGAT CTTGCAAGAC 
ATTTTATACT ACATGGACCG CATTTTTACA GTGATTTTCT TTTTCGAAAT GTTGATAAAG 
TGGCTGGCTT TAGGTTTCCA AAAGTACTTT ACGAACGCAT GGTGCTGGCT AGATTTTATT 
ATCGTAATGG TGTCCTTAAT TAATTTTATT GCCTCTCTTT GTGGCGCTGG TGGTATTCAA 
GCGTTTAAAA CCATGAGAAC ACTCCGAGCT CTGAGGCCGC TCCGTGCCAT GTCGCGGATG 
CAAGGGATGA GGGTTGTGGT GAACGCACTC GTACAAGCTA TACCATCCAT CTTCAATGTA 
CTGCTAGTGT GCTTGATCTT TTGGTTGATT TTTGCTATAA TGGGTGTACA ATTATTTGCT 
GGTAAATACT TTAAGTGCGT GGACGCAAAT AAGACGAGTC TGAGCTACGA AATTATTCCC 
GACTACAACG CTTGTATTGC CGAAAATTAC ACGTGGGAGA ATTCCAGAAT GAATTTTGAT 
CACGTGGGTA AAGCCTACCT TTGCTTGTTT CAAGTCGCCA CGTTTAAGGG CTGGATACAA 
ATCATGAACG ATGCGATAGA TTCCAGAGAG ATTAATAAAC AACCAATAAG AGAGACGAAC 
ATTTACATGT ATTTATATTT CGTCTTCTTT ATTATTTTTG GATCGTTCTT TACTTTGAAT 
CTGTTTATTG GGGTGATCAT TGATAACTTT AATGCACAAA AGAAGAAAGC CGGCGGTTCG 
TTGGAAATGT TCATGACTGA GGATCAGAAA AAGTACTACA ATGCGATGAA GAAAATGGGT 
TCGAAGAAGC CTATGAAAGC GATCCCGAGA CCGAGGTGGA AGCCTCAAGC TATCGTGTTT 
GAGATAGTAA CAAATAAGAA ATTTGACATG ATAATCATGT TGTTCATTGG CTTGAACATG 
TTGACGATGA CGATGGACCA TTACCAGCAG AAAGAGACGT TTACGCAAGT GCTAGACTAC 
CTCAACATGA TTTTCATAGT TATATTTAGT ACCGAGTGCC TCATGAAGAT GTTTGCGTTG 
CGATACCATT ACTTCACGGA ACCGTGGAAT CTCTTCGATT TGGTAGTTGT GATTTTGTCA 
ATCTTGGGTT TAGTTTTGAG TGATATCATC GAAAAATATT TCGTATCACC AACACTGTTG 
AGAGTGGTGC GTGTGGCAAA AGTCGGGAGG GTACTCCGTT TGGTGAAAGG AGCAAAAGGC 
ATCCGCACAC TGCTTTTCGC ATTAGCCATG TCACTGCCTG CACTATTCAA CATCTGTTTA 
CTGTTATTCT TGGTGATGTT CATCTTTGCC ATCTTCGGGA TGTCCTTCTT CATGCACGTC 
AAAGACAAGA GCGGATTGGA TGACGTCTAC AACTTCAAAA CTTTTGCCCA GTCCATGATT 
CTCCTATTTC AGATGTCCAC TTCGGCCGGG TGGGACGGCG TTTTGGACGG GATTATAAAC 
GAAGAGGACT GCAAGTTGCC GAACAATGAG ATAGGAGAGA CGGGGAACTG CGGGAACTCA 
ACAATAGGCA TCGCCTTTCT ACTTAGCTAC CTCGTGATCT CTTTCCTGAT CGTCATCAAC 
ATGTACATCG CCGTGATCTT GGAGAACTAC TCCCAGGCCA CGGAAGACGT GCAGGAGGGC 
TTGACCGACG ACGACTACGA CATGTACTAC GAGATCTGGC AGCAGTTCGA CCCTGACGGC 
ACCCAGTACA TCCGCTACGA CCAGCTGTCG GACTTCCTGG ATGTGCTGGA GCCGCCTTTG 
CAAATCCACA AACCCAACAA ATACAAGATA GTTTCCATGG ACATCACCAT ATGCAAGGGT 
GATCTCATGT TTTGTGTAGA TATTTTAGAT GCGCTAACTA AAGACTTCTT CGCCCGCAAG 
GGCAATCCGA TTGAAGAGAC CGCCGAACTC AGTGAAGTGA ACACTCACCC GAACGAGCCG 
GGCTACGAAC CAGTGAGCTC CACGCTGTGG AGGCAGCGCG AGGAGTACTG TGCGCGCCTG 
ATCCAGAACG CGTGGCGCAA GCACAAGCAG CAGCGCAGCA GCGGCGTGCC CGAGCCGGCA 
GGCGAGGGGG AGCTGGACAA AGACAGTGAG CTGGAGGCGC ACCAAACGGC CGTTTTGGTG 
GAGAACGACG GCTTTGCGAC CAAAAACGGG CACAAAGTGG TGTTGCACAG TCGCACGCCC 
AGCGTCAGCT CCAGGTCTGC GGACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015982008.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837494.1 Tribolium castaneum paralytic A (Para), transcript variant X5, mRNA.

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CloneID	OTg16169
Clone ID Related Accession (Same CDS sequence)	XM_015982008.1
Accession Version	XM_015982008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGC 
CCCCAACCTG ACCCAACTCT GGAGCAAGGG TTACCCATAC CGGTCCGTCT TCAGGGTGGG 
TTTCCCCCGG AACTGGCCAG CACACCACTT GAAGACATCG ACCCTTTTTA CAGCAACCAA 
AGAACTTTTG TAGTAGTTAG TAAAGGCAAG GACATATTCC GATTTAGTGC AACCAACGCT 
CTGTGGATAC TCGATCCTTT CAATCCAATC CGTCGAGTAG CCATTTATAT CTTAGTGCAT 
CCTTTATTTT CTCTTTTCAT CATCACCACC ATCCTCGTCA ATTGTATCCT CATGATTATG 
CCCACCACAC CCACCGTAGA ATCAACTGAA GTAATTTTCA CTGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC CAGAGGTTTT ATTCTTCAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAATT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTTACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACGAT GTTTTCGCTG TCAGTGTTCG CACTGATGGG TTTACAGATT 
TACATGGGGG TTCTGACGCA AAAATGTATC AAAAATTTCC CCATGGACGG CTCCTGGGGA 
AATCTCACCG CTGAAAACTG GGAAAGATTT GTAAGCAACG AAACGAATTG GTATTGGGAC 
GAAGAAAAGG GGGAGCGGCC TTTATGCGGA AACTCCTCCG GCGCCGGCAC ATGTAAACCT 
GGTTATACAT GTTTACAAGG CTATGGTGAA AATCCAAATT ATGGGTACAC AAGTTTTGAT 
ACATTTGGCT GGGCCTTCCT TTCTGCCTTT AGACTAATGA CTCAGGATTA TTGGGAAAAT 
TTATATCAAT TGGTTTTGAG ATCTGCAGGA CCTTGGCACA TGTTGTTTTT TATCGTGATC 
ATCTTCTTGG GCTCGTTTTA TTTGGTCAAC TTAATTTTGG CCATCGTCGC TATGTCTTAT 
GATGAATTAC AAAAGAAGGC TCAGGATGAA GAAGAGGCCG AAGCCGAAGC CATCAGAGAA 
GCTGAAGAGA AGGCGAAGGA GAAGCAGGAC CGGGCGGACG CGCGAGCCTT GGCGGCGGTG 
GAGGCAGCTG CGGCCGCGGA AGCGGCTGCC AGTGGCGGCG GCCACGAGAT CGTGAAGTCT 
TCGTCCGACT ATTCCTGTCA TAGCTATGAA AGCTTCGTAG GACAAGCGAG CGGCCAGGAT 
GACAACAACA AGGAAAAAAT AAGCATACGA TCTGAAGGAT TAGATTCCGT GAGTGAACAC 
AGAAGAGTTC CTAATCTCAG CAAGATACGG AAAGTCAGCG CTGCAAGTCT CAGTCTTCCG 
GGATCTCCGT TCAACCTTCG CAGAGGGTCC AGAGGCAGCC ACCAGTTCAC CATCCGGAAC 
AACAGGCACA TGGTGGGCGG CCCCTGCGAC CGCAAACCTT TGGTTCTCTC GACCTATCTC 
GATGCTCAAG AACATCTTCC CTACGCCGAC GACTCCAACG CTGTCACTCC CATGAGCGAG 
GAGAACGGCG CCATGGTGTT CCCCATGTAC TACGCCAATC TGGGGTCACG TCACTCATCG 
TACAACTCCC ACGCCGCGCG GATGTCCTAC ACCTCGCACG GGGACCTCCT TGGAGGCCTC 
GTAGGCAGTG GGAAAAACAT GACGAAGGAG AGCCAACTCC GGTGCCGTTC CATGAGGAAC 
GCACCTCCGG CCACAACAAA CTCGTTCTTT GAAATCACAC ACAAAGCACA CCGAGGTGAT 
TATGATGGTC CCACGGGTCA GCTTTGTGAA TCGAAATTAA AACAACTGGA TAGTTCCTTT 
ATTGACAACA ATCAGAGGCA AACGGTTGTA GATATGAAAG ACGTAAAAGT CCTCAACGAT 
ATTATTGAGC AAGCGGCTGC CAGCGGCGGG AGTGAGCATG GAGTTTCCGT TTATATTTTC 
TCAGCGACTA ATAACGACGA GAATGACGAA GAAGAGGAGG AGCCCACGGT CGGCGAACGC 
ATGCTGGCCT ACACCCTCAA AATAATCGAC ATTTTCTGCG TCTGGGACTG TTGCCACTGC 
TGGGTCGTCA TCCAGAAGTT CGTCAGTTTG ATAGTGTTTG ACCCGTTCGT CGAGCTGTTC 
ATCACGCTCT GCATCGTCGT CAACACCCTG TTCATGGCGC TCGACCACCA CGACATGGAC 
AAAGACCTGG AAAAGGCCCT AAAGAGCGGC AACTACTTCT TCACCGCCAC TTTCATGATC 
GAAGCTACGA TGAAGTTGAT CGCGATGAGT CCGAAGTTCT ACTTTCAAGA AGGCTGGAAT 
ATCTTTGATT TCATAATAGT GGCGTTGTCG TTGCTGGAAC TTGGGCTTGA AGGGGTCCAG 
GGGTTGTCCG TTTTGAGATC ATTCCGACTG TTGAGGGTTT TTAAACTGGC AAAGTCGTGG 
CCAACCTTGA ACTTACTCAT CTCCATTATG GGCAGGACGA TGGGGGCTTT GGGGAATCTA 
ACGTTTGTCT TGTGCATCAT CATATTCATA TTTGCCGTAA TGGGGATGCA ACTATTTGGG 
AAAAACTATA CAGATAACGT TGATAGATTT CCCGACCACG AACTTCCAAG GTGGAACTTT 
ACCGATTTTA TGCATTCCTT TATGATAGTG TTTAGAGTAC TGTGTGGAGA GTGGATAGAA 
TCTATGTGGG ATTGCATGTT AGTAGGTGAT GTGTCTTGTA TTCCATTCTT CCTAGCAACT 
GTGGTAATTG GTAATCTTGT GGTACTGAAC CTCTTCTTGG CTCTGCTTTT GAGCAACTTT 
GGCTCTTCAA ACCTCTCAGC ACCGACTGCC GACAACGACA CTAATAAAAT AGCAGAGGCC 
TTTGAGAGAA TAGCGCGTTT TATACGGTGG ATAAAGGGTA GTGTTTTAGA CATGGTAAAA 
ATCGTCCGAT TTAAACTAAC TAATCAGATA TCAGACCAGC CCTCAGACGA ACGAGAGTGT 
GCGTTAGAAA TATCAGAAGA CGAAATATTG GCTAACGGTT TAATGAAGAA AAACCCTAAA 
GACCGGGTTG AAGTAACGAT AGGCATAGGG ACCGGTATGG ACCTTACAGT TCATGGTGAT 
TCGAAAGCGA ATTTGAAACG AGACAAGAAC AGCTTGAGTA AAAGCAAAAC TATCGGAAAT 
TCAATTTTAG ACCACAGTGA TTTTATTAGT CACTTGGACG ACGACGAAAT TAGTAATAAT 
TCATACGGGA GTCACACTTA TCGCTTTAAG GACGAGAGTC ACAAAGGCAG TGCCGACGTT 
CTTGAAGATA ACGAAGAGCA GGAAGAGAAG CGCGATGCTA GCAAAGAAGA GCTAGGTATC 
GCCGAAGACA CGGAAGAAGA AGGCTGCGAA TGTAAGGAAG TCCTGAATGA AGATTTGGTT 
GACGCAAACA CTGAAGACAT CATCGACGAA TATTCCGCCG ACTGCTGTCC CGAGTCGTGT 
TACAAAAAGT TCCCGTTCCT GGCCGGCGAC GAAGACTCGC CCTTTTGGCA AGGCTGGGCG 
AACTTGCGCT ACAAAACGTT CCGGCTGATA GAAAACAAAT ATTTCGAAAC GGCGGTCATC 
ACGATGATTT TGCTGAGCAG TCTGGCCCTG GCTTTGGAGG ATGTGCATCT TTCGAAACGG 
CCGATCTTGC AAGACATTTT ATACTACATG GACCGCATTT TTACAGTGAT TTTCTTTTTC 
GAAATGTTGA TAAAGTGGCT GGCTTTAGGT TTCCAAAAGT ACTTTACGAA CGCATGGTGC 
TGGCTAGATT TTATTATCGT AATGGTGTCC TTAATTAATT TTATTGCCTC TCTTTGTGGC 
GCTGGTGGTA TTCAAGCGTT TAAAACCATG AGAACACTCC GAGCTCTGAG GCCGCTCCGT 
GCCATGTCGC GGATGCAAGG GATGAGGGTT GTGGTGAACG CACTCGTACA AGCTATACCA 
TCCATCTTCA ATGTACTGCT AGTGTGCTTG ATCTTTTGGT TGATTTTTGC TATAATGGGT 
GTACAATTAT TTGCTGGTAA ATACTTTAAG TGCGTGGACG CAAATAAGAC GAGTCTGAGC 
TACGAAATTA TTCCCGACTA CAACGCTTGT ATTGCCGAAA ATTACACGTG GGAGAATTCC 
AGAATGAATT TTGATCACGT GGGTAAAGCC TACCTTTGCT TGTTTCAAGT CGCCACGTTT 
AAGGGCTGGA TACAAATCAT GAACGATGCG ATAGATTCCA GAGAGATTAA TAAACAACCA 
ATAAGAGAGA CGAACATTTA CATGTATTTA TATTTCGTCT TCTTTATTAT TTTTGGATCG 
TTCTTTACTT TGAATCTGTT TATTGGGGTG ATCATTGATA ACTTTAATGC ACAAAAGAAG 
AAAGCCGGCG GTTCGTTGGA AATGTTCATG ACTGAGGATC AGAAAAAGTA CTACAATGCG 
ATGAAGAAAA TGGGTTCGAA GAAGCCTATG AAAGCGATCC CGAGACCGAG GTGGAAGCCT 
CAAGCTATCG TGTTTGAGAT AGTAACAAAT AAGAAATTTG ACATGATAAT CATGTTGTTC 
ATTGGCTTGA ACATGTTGAC GATGACGATG GACCATTACC AGCAGAAAGA GACGTTTACG 
CAAGTGCTAG ACTACCTCAA CATGATTTTC ATAGTTATAT TTAGTACCGA GTGCCTCATG 
AAGATGTTTG CGTTGCGATA CCATTACTTC ACGGAACCGT GGAATCTCTT CGATTTGGTA 
GTTGTGATTT TGTCAATCTT GGGTTTAGTT TTGAGTGATA TCATCGAAAA ATATTTCGTA 
TCACCAACAC TGTTGAGAGT GGTGCGTGTG GCAAAAGTCG GGAGGGTACT CCGTTTGGTG 
AAAGGAGCAA AAGGCATCCG CACACTGCTT TTCGCATTAG CCATGTCACT GCCTGCACTA 
TTCAACATCT GTTTACTGTT ATTCTTGGTG ATGTTCATCT TTGCCATCTT CGGGATGTCC 
TTCTTCATGC ACGTCAAAGA CAAGAGCGGA TTGGATGACG TCTACAACTT CAAAACTTTT 
GCCCAGTCCA TGATTCTCCT ATTTCAGATG TCCACTTCGG CCGGGTGGGA CGGCGTTTTG 
GACGGGATTA TAAACGAAGA GGACTGCAAG TTGCCGAACA ATGAGATAGG AGAGACGGGG 
AACTGCGGGA ACTCAACAAT AGGCATCGCC TTTCTACTTA GCTACCTCGT GATCTCTTTC 
CTGATCGTCA TCAACATGTA CATCGCCGTG ATCTTGGAGA ACTACTCCCA GGCCACGGAA 
GACGTGCAGG AGGGCTTGAC CGACGACGAC TACGACATGT ACTACGAGAT CTGGCAGCAG 
TTCGACCCTG ACGGCACCCA GTACATCCGC TACGACCAGC TGTCGGACTT CCTGGATGTG 
CTGGAGCCGC CTTTGCAAAT CCACAAACCC AACAAATACA AGATAGTTTC CATGGACATC 
ACCATATGCA AGGGTGATCT CATGTTTTGT GTAGATATTT TAGATGCGCT AACTAAAGAC 
TTCTTCGCCC GCAAGGGCAA TCCGATTGAA GAGACCGCCG AACTCAGTGA AGTGAACACT 
CACCCGAACG AGCCGGGCTA CGAACCAGTG AGCTCCACGC TGTGGAGGCA GCGCGAGGAG 
TACTGTGCGC GCCTGATCCA GAACGCGTGG CGCAAGCACA AGCAGCAGCG CAGCAGCGGC 
GTGCCCGAGC CGGCAGGCGA GGGGGAGCTG GACAAAGACA GTGAGCTGGA GGCGCACCAA 
ACGGCCGTTT TGGTGGAGAA CGACGGCTTT GCGACCAAAA ACGGGCACAA AGTGGTGTTG 
CACAGTCGCA CGCCCAGCGT CAGCTCCAGG TCTGCGGACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837494.1
CDS	2126..8230
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSVSEHRRVPNLSKIRKVSAASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015982008.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015982008.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGATT CGGTATGAGG ACGAGGACGA AGATGAAGGC 
CCCCAACCTG ACCCAACTCT GGAGCAAGGG TTACCCATAC CGGTCCGTCT TCAGGGTGGG 
TTTCCCCCGG AACTGGCCAG CACACCACTT GAAGACATCG ACCCTTTTTA CAGCAACCAA 
AGAACTTTTG TAGTAGTTAG TAAAGGCAAG GACATATTCC GATTTAGTGC AACCAACGCT 
CTGTGGATAC TCGATCCTTT CAATCCAATC CGTCGAGTAG CCATTTATAT CTTAGTGCAT 
CCTTTATTTT CTCTTTTCAT CATCACCACC ATCCTCGTCA ATTGTATCCT CATGATTATG 
CCCACCACAC CCACCGTAGA ATCAACTGAA GTAATTTTCA CTGGCATCTA CACGTTTGAA 
TCAGCTGTGA AGGTGATGGC CAGAGGTTTT ATTCTTCAAC CGTTCACCTA CCTTAGAGAT 
GCATGGAATT GGCTCGACTT CGTAGTCATA GCTTTAGCTT ATGTTACCAT GGGTATAGAC 
CTTGGGAATC TTGCTGCCTT GAGAACATTT AGGGTACTAC GAGCTCTAAA AACTGTTGCC 
ATTGTGCCAG GTTTGAAGAC TATCGTGGGA GCTGTAATAG AATCTGTAAA AAATCTCCGA 
GATGTGATAA TTTTAACGAT GTTTTCGCTG TCAGTGTTCG CACTGATGGG TTTACAGATT 
TACATGGGGG TTCTGACGCA AAAATGTATC AAAAATTTCC CCATGGACGG CTCCTGGGGA 
AATCTCACCG CTGAAAACTG GGAAAGATTT GTAAGCAACG AAACGAATTG GTATTGGGAC 
GAAGAAAAGG GGGAGCGGCC TTTATGCGGA AACTCCTCCG GCGCCGGCAC ATGTAAACCT 
GGTTATACAT GTTTACAAGG CTATGGTGAA AATCCAAATT ATGGGTACAC AAGTTTTGAT 
ACATTTGGCT GGGCCTTCCT TTCTGCCTTT AGACTAATGA CTCAGGATTA TTGGGAAAAT 
TTATATCAAT TGGTTTTGAG ATCTGCAGGA CCTTGGCACA TGTTGTTTTT TATCGTGATC 
ATCTTCTTGG GCTCGTTTTA TTTGGTCAAC TTAATTTTGG CCATCGTCGC TATGTCTTAT 
GATGAATTAC AAAAGAAGGC TCAGGATGAA GAAGAGGCCG AAGCCGAAGC CATCAGAGAA 
GCTGAAGAGA AGGCGAAGGA GAAGCAGGAC CGGGCGGACG CGCGAGCCTT GGCGGCGGTG 
GAGGCAGCTG CGGCCGCGGA AGCGGCTGCC AGTGGCGGCG GCCACGAGAT CGTGAAGTCT 
TCGTCCGACT ATTCCTGTCA TAGCTATGAA AGCTTCGTAG GACAAGCGAG CGGCCAGGAT 
GACAACAACA AGGAAAAAAT AAGCATACGA TCTGAAGGAT TAGATTCCGT GAGTGAACAC 
AGAAGAGTTC CTAATCTCAG CAAGATACGG AAAGTCAGCG CTGCAAGTCT CAGTCTTCCG 
GGATCTCCGT TCAACCTTCG CAGAGGGTCC AGAGGCAGCC ACCAGTTCAC CATCCGGAAC 
AACAGGCACA TGGTGGGCGG CCCCTGCGAC CGCAAACCTT TGGTTCTCTC GACCTATCTC 
GATGCTCAAG AACATCTTCC CTACGCCGAC GACTCCAACG CTGTCACTCC CATGAGCGAG 
GAGAACGGCG CCATGGTGTT CCCCATGTAC TACGCCAATC TGGGGTCACG TCACTCATCG 
TACAACTCCC ACGCCGCGCG GATGTCCTAC ACCTCGCACG GGGACCTCCT TGGAGGCCTC 
GTAGGCAGTG GGAAAAACAT GACGAAGGAG AGCCAACTCC GGTGCCGTTC CATGAGGAAC 
GCACCTCCGG CCACAACAAA CTCGTTCTTT GAAATCACAC ACAAAGCACA CCGAGGTGAT 
TATGATGGTC CCACGGGTCA GCTTTGTGAA TCGAAATTAA AACAACTGGA TAGTTCCTTT 
ATTGACAACA ATCAGAGGCA AACGGTTGTA GATATGAAAG ACGTAAAAGT CCTCAACGAT 
ATTATTGAGC AAGCGGCTGC CAGCGGCGGG AGTGAGCATG GAGTTTCCGT TTATATTTTC 
TCAGCGACTA ATAACGACGA GAATGACGAA GAAGAGGAGG AGCCCACGGT CGGCGAACGC 
ATGCTGGCCT ACACCCTCAA AATAATCGAC ATTTTCTGCG TCTGGGACTG TTGCCACTGC 
TGGGTCGTCA TCCAGAAGTT CGTCAGTTTG ATAGTGTTTG ACCCGTTCGT CGAGCTGTTC 
ATCACGCTCT GCATCGTCGT CAACACCCTG TTCATGGCGC TCGACCACCA CGACATGGAC 
AAAGACCTGG AAAAGGCCCT AAAGAGCGGC AACTACTTCT TCACCGCCAC TTTCATGATC 
GAAGCTACGA TGAAGTTGAT CGCGATGAGT CCGAAGTTCT ACTTTCAAGA AGGCTGGAAT 
ATCTTTGATT TCATAATAGT GGCGTTGTCG TTGCTGGAAC TTGGGCTTGA AGGGGTCCAG 
GGGTTGTCCG TTTTGAGATC ATTCCGACTG TTGAGGGTTT TTAAACTGGC AAAGTCGTGG 
CCAACCTTGA ACTTACTCAT CTCCATTATG GGCAGGACGA TGGGGGCTTT GGGGAATCTA 
ACGTTTGTCT TGTGCATCAT CATATTCATA TTTGCCGTAA TGGGGATGCA ACTATTTGGG 
AAAAACTATA CAGATAACGT TGATAGATTT CCCGACCACG AACTTCCAAG GTGGAACTTT 
ACCGATTTTA TGCATTCCTT TATGATAGTG TTTAGAGTAC TGTGTGGAGA GTGGATAGAA 
TCTATGTGGG ATTGCATGTT AGTAGGTGAT GTGTCTTGTA TTCCATTCTT CCTAGCAACT 
GTGGTAATTG GTAATCTTGT GGTACTGAAC CTCTTCTTGG CTCTGCTTTT GAGCAACTTT 
GGCTCTTCAA ACCTCTCAGC ACCGACTGCC GACAACGACA CTAATAAAAT AGCAGAGGCC 
TTTGAGAGAA TAGCGCGTTT TATACGGTGG ATAAAGGGTA GTGTTTTAGA CATGGTAAAA 
ATCGTCCGAT TTAAACTAAC TAATCAGATA TCAGACCAGC CCTCAGACGA ACGAGAGTGT 
GCGTTAGAAA TATCAGAAGA CGAAATATTG GCTAACGGTT TAATGAAGAA AAACCCTAAA 
GACCGGGTTG AAGTAACGAT AGGCATAGGG ACCGGTATGG ACCTTACAGT TCATGGTGAT 
TCGAAAGCGA ATTTGAAACG AGACAAGAAC AGCTTGAGTA AAAGCAAAAC TATCGGAAAT 
TCAATTTTAG ACCACAGTGA TTTTATTAGT CACTTGGACG ACGACGAAAT TAGTAATAAT 
TCATACGGGA GTCACACTTA TCGCTTTAAG GACGAGAGTC ACAAAGGCAG TGCCGACGTT 
CTTGAAGATA ACGAAGAGCA GGAAGAGAAG CGCGATGCTA GCAAAGAAGA GCTAGGTATC 
GCCGAAGACA CGGAAGAAGA AGGCTGCGAA TGTAAGGAAG TCCTGAATGA AGATTTGGTT 
GACGCAAACA CTGAAGACAT CATCGACGAA TATTCCGCCG ACTGCTGTCC CGAGTCGTGT 
TACAAAAAGT TCCCGTTCCT GGCCGGCGAC GAAGACTCGC CCTTTTGGCA AGGCTGGGCG 
AACTTGCGCT ACAAAACGTT CCGGCTGATA GAAAACAAAT ATTTCGAAAC GGCGGTCATC 
ACGATGATTT TGCTGAGCAG TCTGGCCCTG GCTTTGGAGG ATGTGCATCT TTCGAAACGG 
CCGATCTTGC AAGACATTTT ATACTACATG GACCGCATTT TTACAGTGAT TTTCTTTTTC 
GAAATGTTGA TAAAGTGGCT GGCTTTAGGT TTCCAAAAGT ACTTTACGAA CGCATGGTGC 
TGGCTAGATT TTATTATCGT AATGGTGTCC TTAATTAATT TTATTGCCTC TCTTTGTGGC 
GCTGGTGGTA TTCAAGCGTT TAAAACCATG AGAACACTCC GAGCTCTGAG GCCGCTCCGT 
GCCATGTCGC GGATGCAAGG GATGAGGGTT GTGGTGAACG CACTCGTACA AGCTATACCA 
TCCATCTTCA ATGTACTGCT AGTGTGCTTG ATCTTTTGGT TGATTTTTGC TATAATGGGT 
GTACAATTAT TTGCTGGTAA ATACTTTAAG TGCGTGGACG CAAATAAGAC GAGTCTGAGC 
TACGAAATTA TTCCCGACTA CAACGCTTGT ATTGCCGAAA ATTACACGTG GGAGAATTCC 
AGAATGAATT TTGATCACGT GGGTAAAGCC TACCTTTGCT TGTTTCAAGT CGCCACGTTT 
AAGGGCTGGA TACAAATCAT GAACGATGCG ATAGATTCCA GAGAGATTAA TAAACAACCA 
ATAAGAGAGA CGAACATTTA CATGTATTTA TATTTCGTCT TCTTTATTAT TTTTGGATCG 
TTCTTTACTT TGAATCTGTT TATTGGGGTG ATCATTGATA ACTTTAATGC ACAAAAGAAG 
AAAGCCGGCG GTTCGTTGGA AATGTTCATG ACTGAGGATC AGAAAAAGTA CTACAATGCG 
ATGAAGAAAA TGGGTTCGAA GAAGCCTATG AAAGCGATCC CGAGACCGAG GTGGAAGCCT 
CAAGCTATCG TGTTTGAGAT AGTAACAAAT AAGAAATTTG ACATGATAAT CATGTTGTTC 
ATTGGCTTGA ACATGTTGAC GATGACGATG GACCATTACC AGCAGAAAGA GACGTTTACG 
CAAGTGCTAG ACTACCTCAA CATGATTTTC ATAGTTATAT TTAGTACCGA GTGCCTCATG 
AAGATGTTTG CGTTGCGATA CCATTACTTC ACGGAACCGT GGAATCTCTT CGATTTGGTA 
GTTGTGATTT TGTCAATCTT GGGTTTAGTT TTGAGTGATA TCATCGAAAA ATATTTCGTA 
TCACCAACAC TGTTGAGAGT GGTGCGTGTG GCAAAAGTCG GGAGGGTACT CCGTTTGGTG 
AAAGGAGCAA AAGGCATCCG CACACTGCTT TTCGCATTAG CCATGTCACT GCCTGCACTA 
TTCAACATCT GTTTACTGTT ATTCTTGGTG ATGTTCATCT TTGCCATCTT CGGGATGTCC 
TTCTTCATGC ACGTCAAAGA CAAGAGCGGA TTGGATGACG TCTACAACTT CAAAACTTTT 
GCCCAGTCCA TGATTCTCCT ATTTCAGATG TCCACTTCGG CCGGGTGGGA CGGCGTTTTG 
GACGGGATTA TAAACGAAGA GGACTGCAAG TTGCCGAACA ATGAGATAGG AGAGACGGGG 
AACTGCGGGA ACTCAACAAT AGGCATCGCC TTTCTACTTA GCTACCTCGT GATCTCTTTC 
CTGATCGTCA TCAACATGTA CATCGCCGTG ATCTTGGAGA ACTACTCCCA GGCCACGGAA 
GACGTGCAGG AGGGCTTGAC CGACGACGAC TACGACATGT ACTACGAGAT CTGGCAGCAG 
TTCGACCCTG ACGGCACCCA GTACATCCGC TACGACCAGC TGTCGGACTT CCTGGATGTG 
CTGGAGCCGC CTTTGCAAAT CCACAAACCC AACAAATACA AGATAGTTTC CATGGACATC 
ACCATATGCA AGGGTGATCT CATGTTTTGT GTAGATATTT TAGATGCGCT AACTAAAGAC 
TTCTTCGCCC GCAAGGGCAA TCCGATTGAA GAGACCGCCG AACTCAGTGA AGTGAACACT 
CACCCGAACG AGCCGGGCTA CGAACCAGTG AGCTCCACGC TGTGGAGGCA GCGCGAGGAG 
TACTGTGCGC GCCTGATCCA GAACGCGTGG CGCAAGCACA AGCAGCAGCG CAGCAGCGGC 
GTGCCCGAGC CGGCAGGCGA GGGGGAGCTG GACAAAGACA GTGAGCTGGA GGCGCACCAA 
ACGGCCGTTT TGGTGGAGAA CGACGGCTTT GCGACCAAAA ACGGGCACAA AGTGGTGTTG 
CACAGTCGCA CGCCCAGCGT CAGCTCCAGG TCTGCGGACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Para ( XM_015982050.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015837536.1 Tribolium castaneum paralytic A (Para), transcript variant X6, mRNA.

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CloneID	OTg16170
Clone ID Related Accession (Same CDS sequence)	XM_015982050.1
Accession Version	XM_015982050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	paralytic A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGCAAGTCTC AGTCTTCCGG GATCTCCGTT CAACCTTCGC 
AGAGGGTCCA GAGGCAGCCA CCAGTTCACC ATCCGGAACA ACAGGCACAT GGTGGGCGGC 
CCCTGCGACC GCAAACCTTT GGTTCTCTCG ACCTATCTCG ATGCTCAAGA ACATCTTCCC 
TACGCCGACG ACTCCAACGC TGTCACTCCC ATGAGCGAGG AGAACGGCGC CATGGTGTTC 
CCCATGTACT ACGCCAATCT GGGGTCACGT CACTCATCGT ACAACTCCCA CGCCGCGCGG 
ATGTCCTACA CCTCGCACGG GGACCTCCTT GGAGGCCTCG TAGGCAGTGG GAAAAACATG 
ACGAAGGAGA GCCAACTCCG GTGCCGTTCC ATGAGGAACG CACCTCCGGC CACAACAAAC 
TCGTTCTTTG AAATCACACA CAAAGCACAC CGAGGTGATT ATGATGGTCC CACGGGTCAG 
CTTTGTGAAT CGAAATTAAA ACAACTGGAT AGTTCCTTTA TTGACAACAA TCAGAGGCAA 
ACGGTTGTAG ATATGAAAGA CGTAAAAGTC CTCAACGATA TTATTGAGCA AGCGGCTGCC 
AGCGGCGGGA GTGAGCATGG AGTTTCCGTT TATATTTTCT CAGCGACTAA TAACGACGAG 
AATGACGAAG AAGAGGAGGA GCCCACGGTC GGCGAACGCA TGCTGGCCTA CACCCTCAAA 
ATAATCGACA TTTTCTGCGT CTGGGACTGT TGCCACTGCT GGGTCGTCAT CCAGAAGTTC 
GTCAGTTTGA TAGTGTTTGA CCCGTTCGTC GAGCTGTTCA TCACGCTCTG CATCGTCGTC 
AACACCCTGT TCATGGCGCT CGACCACCAC GACATGGACA AAGACCTGGA AAAGGCCCTA 
AAGAGCGGCA ACTACTTCTT CACCGCCACT TTCATGATCG AAGCTACGAT GAAGTTGATC 
GCGATGAGTC CGAAGTTCTA CTTTCAAGAA GGCTGGAATA TCTTTGATTT CATAATAGTG 
GCGTTGTCGT TGCTGGAACT TGGGCTTGAA GGGGTCCAGG GGTTGTCCGT TTTGAGATCA 
TTCCGACTGT TGAGGGTTTT TAAACTGGCA AAGTCGTGGC CAACCTTGAA CTTACTCATC 
TCCATTATGG GCAGGACGAT GGGGGCTTTG GGGAATCTAA CGTTTGTCTT GTGCATCATC 
ATATTCATAT TTGCCGTAAT GGGGATGCAA CTATTTGGGA AAAACTATAC AGATAACGTT 
GATAGATTTC CCGACCACGA ACTTCCAAGG TGGAACTTTA CCGATTTTAT GCATTCCTTT 
ATGATAGTGT TTAGAGTACT GTGTGGAGAG TGGATAGAAT CTATGTGGGA TTGCATGTTA 
GTAGGTGATG TGTCTTGTAT TCCATTCTTC CTAGCAACTG TGGTAATTGG TAATCTTGTG 
GTACTGAACC TCTTCTTGGC TCTGCTTTTG AGCAACTTTG GCTCTTCAAA CCTCTCAGCA 
CCGACTGCCG ACAACGACAC TAATAAAATA GCAGAGGCCT TTGAGAGAAT AGCGCGTTTT 
ATACGGTGGA TAAAGGGTAG TGTTTTAGAC ATGGTAAAAA TCGTCCGATT TAAACTAACT 
AATCAGATAT CAGACCAGCC CTCAGGTAAA AAAAGAAACT TATCTCTAGT TAGTCACTTG 
GTAATTTCAG ACGAACGAGA GTGTGCGTTA GAAATATCAG AAGACGAAAT ATTGGCTAAC 
GGTTTAATGA AGAAAAACCC TAAAGACCGG GTTGAAGTAA CGATAGGCAT AGGGACCGGT 
ATGGACCTTA CAGTTCATGG TGATTCGAAA GCGAATTTGA AACGAGACAA GAACAGCTTG 
AGTAAAAGCA AAACTATCGG AAATTCAATT TTAGACCACA GTGATTTTAT TAGTCACTTG 
GACGACGACG AAATTAGTAA TAATTCATAC GGGAGTCACA CTTATCGCTT TAAGGACGAG 
AGTCACAAAG GCAGTGCCGA CGTTCTTGAA GATAACGAAG AGCAGGAAGA GAAGCGCGAT 
GCTAGCAAAG AAGAGCTAGG TATCGCCGAA GACACGGAAG AAGAAGGCTG CGAATGTAAG 
GAAGTCCTGA ATGAAGATTT GGTTGACGCA AACACTGAAG ACATCATCGA CGAATATTCC 
GCCGACTGCT GTCCCGAGTC GTGTTACAAA AAGTTCCCGT TCCTGGCCGG CGACGAAGAC 
TCGCCCTTTT GGCAAGGCTG GGCGAACTTG CGCTACAAAA CGTTCCGGCT GATAGAAAAC 
AAATATTTCG AAACGGCGGT CATCACGATG ATTTTGCTGA GCAGTCTGGC CCTGGCTTTG 
GAGGATGTGC ATCTTTCGAA ACGGCCGATC TTGCAAGACA TTTTATACTA CATGGACCGC 
ATTTTTACAG TGATTTTCTT TTTCGAAATG TTGATAAAGT GGCTGGCTTT AGGTTTCCAA 
AAGTACTTTA CGAACGCATG GTGCTGGCTA GATTTTATTA TCGTAATGGT GTCCTTAATT 
AATTTTATTG CCTCTCTTTG TGGCGCTGGT GGTATTCAAG CGTTTAAAAC CATGAGAACA 
CTCCGAGCTC TGAGGCCGCT CCGTGCCATG TCGCGGATGC AAGGGATGAG GGTTGTGGTG 
AACGCACTCG TACAAGCTAT ACCATCCATC TTCAATGTAC TGCTAGTGTG CTTGATCTTT 
TGGTTGATTT TTGCTATAAT GGGTGTACAA TTATTTGCTG GTAAATACTT TAAGTGCGTG 
GACGCAAATA AGACGAGTCT GAGCTACGAA ATTATTCCCG ACTACAACGC TTGTATTGCC 
GAAAATTACA CGTGGGAGAA TTCCAGAATG AATTTTGATC ACGTGGGTAA AGCCTACCTT 
TGCTTGTTTC AAGTCGCCAC GTTTAAGGGC TGGATACAAA TCATGAACGA TGCGATAGAT 
TCCAGAGAGA TTAATAAACA ACCAATAAGA GAGACGAACA TTTACATGTA TTTATATTTC 
GTCTTCTTTA TTATTTTTGG ATCGTTCTTT ACTTTGAATC TGTTTATTGG GGTGATCATT 
GATAACTTTA ATGCACAAAA GAAGAAAGCC GGCGGTTCGT TGGAAATGTT CATGACTGAG 
GATCAGAAAA AGTACTACAA TGCGATGAAG AAAATGGGTT CGAAGAAGCC TATGAAAGCG 
ATCCCGAGAC CGAGGTGGAA GCCTCAAGCT ATCGTGTTTG AGATAGTAAC AAATAAGAAA 
TTTGACATGA TAATCATGTT GTTCATTGGC TTGAACATGT TGACGATGAC GATGGACCAT 
TACCAGCAGA AAGAGACGTT TACGCAAGTG CTAGACTACC TCAACATGAT TTTCATAGTT 
ATATTTAGTA CCGAGTGCCT CATGAAGATG TTTGCGTTGC GATACCATTA CTTCACGGAA 
CCGTGGAATC TCTTCGATTT GGTAGTTGTG ATTTTGTCAA TCTTGGGTTT AGTTTTGAGT 
GATATCATCG AAAAATATTT CGTATCACCA ACACTGTTGA GAGTGGTGCG TGTGGCAAAA 
GTCGGGAGGG TACTCCGTTT GGTGAAAGGA GCAAAAGGCA TCCGCACACT GCTTTTCGCA 
TTAGCCATGT CACTGCCTGC ACTATTCAAC ATCTGTTTAC TGTTATTCTT GGTGATGTTC 
ATCTTTGCCA TCTTCGGGAT GTCCTTCTTC ATGCACGTCA AAGACAAGAG CGGATTGGAT 
GACGTCTACA ACTTCAAAAC TTTTGCCCAG TCCATGATTC TCCTATTTCA GATGTCCACT 
TCGGCCGGGT GGGACGGCGT TTTGGACGGG ATTATAAACG AAGAGGACTG CAAGTTGCCG 
AACAATGAGA TAGGAGAGAC GGGGAACTGC GGGAACTCAA CAATAGGCAT CGCCTTTCTA 
CTTAGCTACC TCGTGATCTC TTTCCTGATC GTCATCAACA TGTACATCGC CGTGATCTTG 
GAGAACTACT CCCAGGCCAC GGAAGACGTG CAGGAGGGCT TGACCGACGA CGACTACGAC 
ATGTACTACG AGATCTGGCA GCAGTTCGAC CCTGACGGCA CCCAGTACAT CCGCTACGAC 
CAGCTGTCGG ACTTCCTGGA TGTGCTGGAG CCGCCTTTGC AAATCCACAA ACCCAACAAA 
TACAAGATAG TTTCCATGGA CATCACCATA TGCAAGGGTG ATCTCATGTT TTGTGTAGAT 
ATTTTAGATG CGCTAACTAA AGACTTCTTC GCCCGCAAGG GCAATCCGAT TGAAGAGACC 
GCCGAACTCA GTGAAGTGAA CACTCACCCG AACGAGCCGG GCTACGAACC AGTGAGCTCC 
ACGCTGTGGA GGCAGCGCGA GGAGTACTGT GCGCGCCTGA TCCAGAACGC GTGGCGCAAG 
CACAAGCAGC AGCGCAGCAG CGGCGTGCCC GAGCCGGCAG GCGAGGGGGA GCTGGACAAA 
GACAGTGAGC TGGAGGCGCA CCAAACGGCC GTTTTGGTGG AGAACGACGG CTTTGCGACC 
AAAAACGGGC ACAAAGTGGT GTTGCACAGT CGCACGCCCA GCGTCAGCTC CAGGTCTGCG 
GACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015837536.1
CDS	2126..8254
Translation	MSDASDLSSEEERSLFRPFTRESLATIEARIAEEHAKQKELEKKRAEGETGFGRRKKKKEIRYEDEDEDEGPQPDPTLEQGLPIPVRLQGGFPPELASTPLEDIDPFYSNQRTFVVVSKGKDIFRFSATNALWILDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKNFPMDGSWGNLTAENWERFVSNETNWYWDEEKGERPLCGNSSGAGTCKPGYTCLQGYGENPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAQDEEEAEAEAIREAEEKAKEKQDRADARALAAVEAAAAAEAAASGGGHEIVKSSSDYSCHSYESFVGQASGQDDNNKEKISIRSEGLDSASLSLPGSPFNLRRGSRGSHQFTIRNNRHMVGGPCDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAMVFPMYYANLGSRHSSYNSHAARMSYTSHGDLLGGLVGSGKNMTKESQLRCRSMRNAPPATTNSFFEITHKAHRGDYDGPTGQLCESKLKQLDSSFIDNNQRQTVVDMKDVKVLNDIIEQAAASGGSEHGVSVYIFSATNNDENDEEEEEPTVGERMLAYTLKIIDIFCVWDCCHCWVVIQKFVSLIVFDPFVELFITLCIVVNTLFMALDHHDMDKDLEKALKSGNYFFTATFMIEATMKLIAMSPKFYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHELPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSNLSAPTADNDTNKIAEAFERIARFIRWIKGSVLDMVKIVRFKLTNQISDQPSGKKRNLSLVSHLVISDERECALEISEDEILANGLMKKNPKDRVEVTIGIGTGMDLTVHGDSKANLKRDKNSLSKSKTIGNSILDHSDFISHLDDDEISNNSYGSHTYRFKDESHKGSADVLEDNEEQEEKRDASKEELGIAEDTEEEGCECKEVLNEDLVDANTEDIIDEYSADCCPESCYKKFPFLAGDEDSPFWQGWANLRYKTFRLIENKYFETAVITMILLSSLALALEDVHLSKRPILQDILYYMDRIFTVIFFFEMLIKWLALGFQKYFTNAWCWLDFIIVMVSLINFIASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDANKTSLSYEIIPDYNACIAENYTWENSRMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSREINKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNAQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPMKAIPRPRWKPQAIVFEIVTNKKFDMIIMLFIGLNMLTMTMDHYQQKETFTQVLDYLNMIFIVIFSTECLMKMFALRYHYFTEPWNLFDLVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFAQSMILLFQMSTSAGWDGVLDGIINEEDCKLPNNEIGETGNCGNSTIGIAFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYIRYDQLSDFLDVLEPPLQIHKPNKYKIVSMDITICKGDLMFCVDILDALTKDFFARKGNPIEETAELSEVNTHPNEPGYEPVSSTLWRQREEYCARLIQNAWRKHKQQRSSGVPEPAGEGELDKDSELEAHQTAVLVENDGFATKNGHKVVLHSRTPSVSSRSADV

Target ORF information:

RefSeq Version	XM_015982050.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum paralytic A (Para), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015982050.1

ORF Insert Sequence:

ATGTCCGACG CCTCTGACTT ATCGTCCGAG GAGGAGCGCA GCCTATTCCG TCCATTTACG 
CGCGAGTCGC TTGCGACCAT CGAGGCTCGA ATTGCAGAAG AACATGCTAA ACAGAAGGAG 
TTGGAAAAGA AAAGAGCTGA AGGAGAGACC GGTTTTGGAC GCAGGAAAAA GAAAAAAGAA 
ATTCGGTATG AGGACGAGGA CGAAGATGAA GGCCCCCAAC CTGACCCAAC TCTGGAGCAA 
GGGTTACCCA TACCGGTCCG TCTTCAGGGT GGGTTTCCCC CGGAACTGGC CAGCACACCA 
CTTGAAGACA TCGACCCTTT TTACAGCAAC CAAAGAACTT TTGTAGTAGT TAGTAAAGGC 
AAGGACATAT TCCGATTTAG TGCAACCAAC GCTCTGTGGA TACTCGATCC TTTCAATCCA 
ATCCGTCGAG TAGCCATTTA TATCTTAGTG CATCCTTTAT TTTCTCTTTT CATCATCACC 
ACCATCCTCG TCAATTGTAT CCTCATGATT ATGCCCACCA CACCCACCGT AGAATCAACT 
GAAGTAATTT TCACTGGCAT CTACACGTTT GAATCAGCTG TGAAGGTGAT GGCCAGAGGT 
TTTATTCTTC AACCGTTCAC CTACCTTAGA GATGCATGGA ATTGGCTCGA CTTCGTAGTC 
ATAGCTTTAG CTTATGTTAC CATGGGTATA GACCTTGGGA ATCTTGCTGC CTTGAGAACA 
TTTAGGGTAC TACGAGCTCT AAAAACTGTT GCCATTGTGC CAGGTTTGAA GACTATCGTG 
GGAGCTGTAA TAGAATCTGT AAAAAATCTC CGAGATGTGA TAATTTTAAC GATGTTTTCG 
CTGTCAGTGT TCGCACTGAT GGGTTTACAG ATTTACATGG GGGTTCTGAC GCAAAAATGT 
ATCAAAAATT TCCCCATGGA CGGCTCCTGG GGAAATCTCA CCGCTGAAAA CTGGGAAAGA 
TTTGTAAGCA ACGAAACGAA TTGGTATTGG GACGAAGAAA AGGGGGAGCG GCCTTTATGC 
GGAAACTCCT CCGGCGCCGG CACATGTAAA CCTGGTTATA CATGTTTACA AGGCTATGGT 
GAAAATCCAA ATTATGGGTA CACAAGTTTT GATACATTTG GCTGGGCCTT CCTTTCTGCC 
TTTAGACTAA TGACTCAGGA TTATTGGGAA AATTTATATC AATTGGTTTT GAGATCTGCA 
GGACCTTGGC ACATGTTGTT TTTTATCGTG ATCATCTTCT TGGGCTCGTT TTATTTGGTC 
AACTTAATTT TGGCCATCGT CGCTATGTCT TATGATGAAT TACAAAAGAA GGCTCAGGAT 
GAAGAAGAGG CCGAAGCCGA AGCCATCAGA GAAGCTGAAG AGAAGGCGAA GGAGAAGCAG 
GACCGGGCGG ACGCGCGAGC CTTGGCGGCG GTGGAGGCAG CTGCGGCCGC GGAAGCGGCT 
GCCAGTGGCG GCGGCCACGA GATCGTGAAG TCTTCGTCCG ACTATTCCTG TCATAGCTAT 
GAAAGCTTCG TAGGACAAGC GAGCGGCCAG GATGACAACA ACAAGGAAAA AATAAGCATA 
CGATCTGAAG GATTAGATTC CGCAAGTCTC AGTCTTCCGG GATCTCCGTT CAACCTTCGC 
AGAGGGTCCA GAGGCAGCCA CCAGTTCACC ATCCGGAACA ACAGGCACAT GGTGGGCGGC 
CCCTGCGACC GCAAACCTTT GGTTCTCTCG ACCTATCTCG ATGCTCAAGA ACATCTTCCC 
TACGCCGACG ACTCCAACGC TGTCACTCCC ATGAGCGAGG AGAACGGCGC CATGGTGTTC 
CCCATGTACT ACGCCAATCT GGGGTCACGT CACTCATCGT ACAACTCCCA CGCCGCGCGG 
ATGTCCTACA CCTCGCACGG GGACCTCCTT GGAGGCCTCG TAGGCAGTGG GAAAAACATG 
ACGAAGGAGA GCCAACTCCG GTGCCGTTCC ATGAGGAACG CACCTCCGGC CACAACAAAC 
TCGTTCTTTG AAATCACACA CAAAGCACAC CGAGGTGATT ATGATGGTCC CACGGGTCAG 
CTTTGTGAAT CGAAATTAAA ACAACTGGAT AGTTCCTTTA TTGACAACAA TCAGAGGCAA 
ACGGTTGTAG ATATGAAAGA CGTAAAAGTC CTCAACGATA TTATTGAGCA AGCGGCTGCC 
AGCGGCGGGA GTGAGCATGG AGTTTCCGTT TATATTTTCT CAGCGACTAA TAACGACGAG 
AATGACGAAG AAGAGGAGGA GCCCACGGTC GGCGAACGCA TGCTGGCCTA CACCCTCAAA 
ATAATCGACA TTTTCTGCGT CTGGGACTGT TGCCACTGCT GGGTCGTCAT CCAGAAGTTC 
GTCAGTTTGA TAGTGTTTGA CCCGTTCGTC GAGCTGTTCA TCACGCTCTG CATCGTCGTC 
AACACCCTGT TCATGGCGCT CGACCACCAC GACATGGACA AAGACCTGGA AAAGGCCCTA 
AAGAGCGGCA ACTACTTCTT CACCGCCACT TTCATGATCG AAGCTACGAT GAAGTTGATC 
GCGATGAGTC CGAAGTTCTA CTTTCAAGAA GGCTGGAATA TCTTTGATTT CATAATAGTG 
GCGTTGTCGT TGCTGGAACT TGGGCTTGAA GGGGTCCAGG GGTTGTCCGT TTTGAGATCA 
TTCCGACTGT TGAGGGTTTT TAAACTGGCA AAGTCGTGGC CAACCTTGAA CTTACTCATC 
TCCATTATGG GCAGGACGAT GGGGGCTTTG GGGAATCTAA CGTTTGTCTT GTGCATCATC 
ATATTCATAT TTGCCGTAAT GGGGATGCAA CTATTTGGGA AAAACTATAC AGATAACGTT 
GATAGATTTC CCGACCACGA ACTTCCAAGG TGGAACTTTA CCGATTTTAT GCATTCCTTT 
ATGATAGTGT TTAGAGTACT GTGTGGAGAG TGGATAGAAT CTATGTGGGA TTGCATGTTA 
GTAGGTGATG TGTCTTGTAT TCCATTCTTC CTAGCAACTG TGGTAATTGG TAATCTTGTG 
GTACTGAACC TCTTCTTGGC TCTGCTTTTG AGCAACTTTG GCTCTTCAAA CCTCTCAGCA 
CCGACTGCCG ACAACGACAC TAATAAAATA GCAGAGGCCT TTGAGAGAAT AGCGCGTTTT 
ATACGGTGGA TAAAGGGTAG TGTTTTAGAC ATGGTAAAAA TCGTCCGATT TAAACTAACT 
AATCAGATAT CAGACCAGCC CTCAGGTAAA AAAAGAAACT TATCTCTAGT TAGTCACTTG 
GTAATTTCAG ACGAACGAGA GTGTGCGTTA GAAATATCAG AAGACGAAAT ATTGGCTAAC 
GGTTTAATGA AGAAAAACCC TAAAGACCGG GTTGAAGTAA CGATAGGCAT AGGGACCGGT 
ATGGACCTTA CAGTTCATGG TGATTCGAAA GCGAATTTGA AACGAGACAA GAACAGCTTG 
AGTAAAAGCA AAACTATCGG AAATTCAATT TTAGACCACA GTGATTTTAT TAGTCACTTG 
GACGACGACG AAATTAGTAA TAATTCATAC GGGAGTCACA CTTATCGCTT TAAGGACGAG 
AGTCACAAAG GCAGTGCCGA CGTTCTTGAA GATAACGAAG AGCAGGAAGA GAAGCGCGAT 
GCTAGCAAAG AAGAGCTAGG TATCGCCGAA GACACGGAAG AAGAAGGCTG CGAATGTAAG 
GAAGTCCTGA ATGAAGATTT GGTTGACGCA AACACTGAAG ACATCATCGA CGAATATTCC 
GCCGACTGCT GTCCCGAGTC GTGTTACAAA AAGTTCCCGT TCCTGGCCGG CGACGAAGAC 
TCGCCCTTTT GGCAAGGCTG GGCGAACTTG CGCTACAAAA CGTTCCGGCT GATAGAAAAC 
AAATATTTCG AAACGGCGGT CATCACGATG ATTTTGCTGA GCAGTCTGGC CCTGGCTTTG 
GAGGATGTGC ATCTTTCGAA ACGGCCGATC TTGCAAGACA TTTTATACTA CATGGACCGC 
ATTTTTACAG TGATTTTCTT TTTCGAAATG TTGATAAAGT GGCTGGCTTT AGGTTTCCAA 
AAGTACTTTA CGAACGCATG GTGCTGGCTA GATTTTATTA TCGTAATGGT GTCCTTAATT 
AATTTTATTG CCTCTCTTTG TGGCGCTGGT GGTATTCAAG CGTTTAAAAC CATGAGAACA 
CTCCGAGCTC TGAGGCCGCT CCGTGCCATG TCGCGGATGC AAGGGATGAG GGTTGTGGTG 
AACGCACTCG TACAAGCTAT ACCATCCATC TTCAATGTAC TGCTAGTGTG CTTGATCTTT 
TGGTTGATTT TTGCTATAAT GGGTGTACAA TTATTTGCTG GTAAATACTT TAAGTGCGTG 
GACGCAAATA AGACGAGTCT GAGCTACGAA ATTATTCCCG ACTACAACGC TTGTATTGCC 
GAAAATTACA CGTGGGAGAA TTCCAGAATG AATTTTGATC ACGTGGGTAA AGCCTACCTT 
TGCTTGTTTC AAGTCGCCAC GTTTAAGGGC TGGATACAAA TCATGAACGA TGCGATAGAT 
TCCAGAGAGA TTAATAAACA ACCAATAAGA GAGACGAACA TTTACATGTA TTTATATTTC 
GTCTTCTTTA TTATTTTTGG ATCGTTCTTT ACTTTGAATC TGTTTATTGG GGTGATCATT 
GATAACTTTA ATGCACAAAA GAAGAAAGCC GGCGGTTCGT TGGAAATGTT CATGACTGAG 
GATCAGAAAA AGTACTACAA TGCGATGAAG AAAATGGGTT CGAAGAAGCC TATGAAAGCG 
ATCCCGAGAC CGAGGTGGAA GCCTCAAGCT ATCGTGTTTG AGATAGTAAC AAATAAGAAA 
TTTGACATGA TAATCATGTT GTTCATTGGC TTGAACATGT TGACGATGAC GATGGACCAT 
TACCAGCAGA AAGAGACGTT TACGCAAGTG CTAGACTACC TCAACATGAT TTTCATAGTT 
ATATTTAGTA CCGAGTGCCT CATGAAGATG TTTGCGTTGC GATACCATTA CTTCACGGAA 
CCGTGGAATC TCTTCGATTT GGTAGTTGTG ATTTTGTCAA TCTTGGGTTT AGTTTTGAGT 
GATATCATCG AAAAATATTT CGTATCACCA ACACTGTTGA GAGTGGTGCG TGTGGCAAAA 
GTCGGGAGGG TACTCCGTTT GGTGAAAGGA GCAAAAGGCA TCCGCACACT GCTTTTCGCA 
TTAGCCATGT CACTGCCTGC ACTATTCAAC ATCTGTTTAC TGTTATTCTT GGTGATGTTC 
ATCTTTGCCA TCTTCGGGAT GTCCTTCTTC ATGCACGTCA AAGACAAGAG CGGATTGGAT 
GACGTCTACA ACTTCAAAAC TTTTGCCCAG TCCATGATTC TCCTATTTCA GATGTCCACT 
TCGGCCGGGT GGGACGGCGT TTTGGACGGG ATTATAAACG AAGAGGACTG CAAGTTGCCG 
AACAATGAGA TAGGAGAGAC GGGGAACTGC GGGAACTCAA CAATAGGCAT CGCCTTTCTA 
CTTAGCTACC TCGTGATCTC TTTCCTGATC GTCATCAACA TGTACATCGC CGTGATCTTG 
GAGAACTACT CCCAGGCCAC GGAAGACGTG CAGGAGGGCT TGACCGACGA CGACTACGAC 
ATGTACTACG AGATCTGGCA GCAGTTCGAC CCTGACGGCA CCCAGTACAT CCGCTACGAC 
CAGCTGTCGG ACTTCCTGGA TGTGCTGGAG CCGCCTTTGC AAATCCACAA ACCCAACAAA 
TACAAGATAG TTTCCATGGA CATCACCATA TGCAAGGGTG ATCTCATGTT TTGTGTAGAT 
ATTTTAGATG CGCTAACTAA AGACTTCTTC GCCCGCAAGG GCAATCCGAT TGAAGAGACC 
GCCGAACTCA GTGAAGTGAA CACTCACCCG AACGAGCCGG GCTACGAACC AGTGAGCTCC 
ACGCTGTGGA GGCAGCGCGA GGAGTACTGT GCGCGCCTGA TCCAGAACGC GTGGCGCAAG 
CACAAGCAGC AGCGCAGCAG CGGCGTGCCC GAGCCGGCAG GCGAGGGGGA GCTGGACAAA 
GACAGTGAGC TGGAGGCGCA CCAAACGGCC GTTTTGGTGG AGAACGACGG CTTTGCGACC 
AAAAACGGGC ACAAAGTGGT GTTGCACAGT CGCACGCCCA GCGTCAGCTC CAGGTCTGCG 
GACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Para cDNA ORF clone, Tribolium castaneum(red flour beetle)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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