Cht10 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cht10
Entrez Gene ID	652967
Full Name	chitinase 10
Synonyms	TcCht10
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008199916.2	XP_008198138.1	chitinase 10 isoform X1
NM_001042602.1	NP_001036067.1	chitinase 10 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny, expression and function.
Jasrapuria S, Arakane Y, Osman G, Kramer KJ, Beeman RW, Muthukrishnan S
Insect biochemistry and molecular biology40(3)214-27(2010 Mar)

Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects.
Zhu Q, Arakane Y, Banerjee D, Beeman RW, Kramer KJ, Muthukrishnan S
Insect biochemistry and molecular biology38(4)452-66(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cht10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cht10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg16056	NM_001042602.1 Latest version!	Tribolium castaneum chitinase 10 (Cht10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTg13332	XM_008199916.2 Latest version!	Tribolium castaneum chitinase 10 (Cht10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cht10 ( NM_001042602.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001036067.1 Tribolium castaneum chitinase 10 (Cht10), mRNA.

Price & Availability↑

CloneID	OTg16056
Clone ID Related Accession (Same CDS sequence)	NM_001042602.1
Accession Version	NM_001042602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1535644800000
Organism	Tribolium castaneum(red flour beetle)
Product	chitinase 10 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ659250.1. On Jul 23, 2006 this sequence version replaced XM_969755.1. ##Evidence-Data-START## Transcript exon combination :: DQ659250.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1523770, SAMEA1523771 [ECO:0000350] ##Evidence-Data-END##

ATGGAATTAC TGCACATAAC TCTATGTGCG TTCTTAATCC TCACACCGAC CGACCAAAGG 
GTCGTCACAA GGACAATCCC GAGCTTCCTT CGGGACGACG TCGAGCGATA TCCCTCAGGA 
CCCGAGCTTT CGGACAGGAG CGCCGCTTAC ACAAATCGGG AACTGTACGG GACGCGGGCT 
TTGCCGCTCC GTTCGGCGGT TGAACACATC CCGGATTTGA TTGCGGACTC GCGCCGGCTG 
CCACTCAGGG ACGCGGTGGA GAAACGCCCA CCGCCCGACG AACCAATCGA CGATTACGAA 
CCGAGCTTTG ATCTGTTCTA CTACCCGTCC CGACAAACTC TCTACACCCC AGTCCAGTAT 
TTCAAGGATT TGGTGGCCGA GGCGTACGTT TCCCCCTTTC GCATTGACAA CGAATCGCAG 
GAACTGCTAA AGTACCCCTC CTTTGCCCGG GGGCGGAGTT TGTATGACTC CAGAGGGAGG 
CACTCAGAGA TAGTTGAGAC GGTTTATGAC CCACGGGAGG ATGACCGTGT TGTTTGCTAC 
GTCCAAGCGG CTGCCAGGTA CCGGAAGGAG CCCTTGGCCT TCTCCCCCGA GGATTTGGAC 
CCCTTTGCTT GCACCCATGT CATTTATGCG TTCGCGACCA TTGACCCCCA CAATTTTAAC 
ATGATTTCGA ACGACGACGA GTCTGATATT ATCCAAGGGG GCTATATTTC CGTCACGGGA 
CTCAAAAGAG TTAACCCGAA ACTAAAAGTA CTGATCTCGG TTGGTGAGGG ACGGGATGGT 
TCCCACAGGT TCAGTACCAT GGTCTCCAGT GCCAACAGAC GAAGGGAGTT CATCCGGTCC 
GCCATCACTT TCATCAAGCA GTACAACTTT GACGGAATGG ACATCCACTG GGAGTACCCA 
GGGGCCGAGA AAATGGGCGG GCAACTGAGC GATAAGGAGT ACCTGAACCT CTTCCTCGAG 
GAGTTATCTG AAATTTTCAA ACCCAGAGGT TGGGTACTTA CAATTGCGGT ACCAGCCTCC 
CGGTTCCGGG TCGAAGATGG GTTTAACCCC CAACGCTTGG GGTCCCTTGT CGATTTTATC 
AACCTCCAAG CGTTTGATTT CCACCGGGAG CGGGAACCGG TGGCCGACCA CCACGCAAAT 
TTGTACTCAA GGCCGGGCGA TAGTGGCCTT GACCTGTTCC TAAGTGTGGA CTATGCTGTG 
AAGTTCTGGA CCAAGAAGGG GTTCCCCAAG TCCAAGATCG TGCTCGGGGT ACCATTTTTC 
GGGCGTTCGT TCACCCTCCA GTTCACAAAC GAGACGCAAG TTGGGGCCCC GATTAAAGGT 
CCAGGACGGG AAGGGTTCTA TACGCAAAAT CCAGGCTTCC TGGCATATTT CGAAATCTGC 
GATATGTTAC TGAATGAAGG GTGGCACAAA GGGATGGACG AGTCAGGGTC GCCTTACATG 
GTATATGGGG ACCAATGGGT GGGTTATGAC GATAGCGAAA GCGTCGAAAA GAAGGTTAAT 
TACGTGCAAG AGATGAACCT CGGGGGTGTG TTTATTTGGG CTACGGATCT GGACGATTTT 
AAGGGCGTTT GCGGAATGAA ATGGCCCTTG CTGAGTACCA TTAACCGGCA TTTGAGGGGA 
AACGAGTTGT TACCGATGCA ACAAAGCCAA AACCCTCCCA CTAAACCATA TGGTACTTGC 
CGCTCAGAAG GGATCTTCAG TGACCCCAAG AACTGTGCAG CTTATTACGT TTGTCGCTCA 
GGGTTGAGCT ACCACTTGTC GTGTGCCGAG AACATGATGT TTGACCCAAT GTCCGGGAAA 
TGTGAGTACA GCCTGGGGGA GAAGTGTAGG CCGGGTCAGA TCATCCAAGT CGCTAACTCT 
CTAAGACAAT ATGAGGACCT CCTGGAGTAT GAAGCTAAAG AGGAAGGACC AAAAGTGGTG 
TGCTACATGA CCAACTGGGC CTTCTACCGA AAAGCCGAAG GCAAATTCGT CCCGGAACAC 
ATCGACCAAA GGCTTTGCAC ACACGTGGTG TACGCCTTCG CGAGTCTCGA CCCCGAAAAA 
CTCCTCCTGA AGGAGTTCGA TCCTTGGGCC GACTTGGACA ACAATTTGTA CGAAAGAGTC 
ACCTCTTTGA AGGACACCAA GGCGATTTTA TCTCTTGGAG GCTGGACTGA CTCAGCTGGA 
GACAAGTACT CACGTCTTGT GGGCGATGGT TCCGCCAGAC GCCGGTTTGT TGTGGCAGTT 
GTGGGGTTTT TGCGACGACA CAATTTCAAA GGTTTGCATT TGGATTGGAA CTACCCCGTT 
TGTTGGCAAA GCAATTGCAA AAAAGGGGCG TCCTCCGATA AACCCAACTT CACCAAATTG 
ATTCAGGTAG TACCAACTAT GGAGTTGCGG CGCGAGTTCG ACAAACAGAA ACCTCCCCTG 
ATCCTGGCTG CGGCCATTTC GGGGTATAAG GAGGTGATAG ACGTGGCGTA CGACCTACCA 
GCGCTTGGTA CCGTCCTTGA TTTCATGTCA GTGATGACTT ATGACTACCA TGGGGCTTGG 
GAACGCCAAA CGGGGCACAA CTTCACCATG GAGTATTTGG TGTCTCGTGG TGCTCCCCGT 
GGGAAACTGC TAGTCGGAGT ACCATTCTAT GGTCAAAGTT TTACCCTAAC CAAGGGCAAC 
AACCACGACC AGGGAGTCCC AAGTGCCGGT CCCGGCGAAG CCGGTGAATA CACCAAACAG 
CCAGGAATGT TGGCCTATTA CGAAATTTGT AACCGGATCC GGAACCAACG CTGGATCGTC 
AATCGGGACA CTTCGGGTGC CACCGGCCCA TTTGCCTACC ATGGGGACCA ATGGGTCGGT 
TTCGAGGACA TCAAGTCGGT CCGAGACAAG GCCGAATACA TCAAAACCAA AGGTTTTGGC 
GGCGCCGTGG CCTGGACTAT CGACTTGGAC GACTTCTCCA ACCGTTGTTG CGGTGGTTCC 
TTCCCTTTAC TTCGAAGTTT GAACCGTGGC TTGGGATTAA TCCCCGATAA TCCCTCACGA 
GAGGACTGCA CCAAACCACC GGAACCCGTA ACTCCAGCTC CTCCACAAGT CACAACTGGG 
GTTGATACGG GAGCTTCAAG TACCGAACAC ATGCATCCAG ACTGGACGAC CAAACCCAGC 
ACCTGGTGGT CGAGTACTAC GACAAGTCCG TGGTGGACCA CTACCACAAC GAGGAGGACC 
ACTACGACTC GACCTACCAC GACTAGTACC ACGACAAGGC CAACTACGAC CAATTGGCCG 
ACCCAAGGAA CCACAATCCC GCCCCCGGCT GTTGTAATGC CAGAAGTGGA TAAACCATCA 
CAACCATGCG AACCTGGGCA ATACGTACCA GACCCACACA ATTGTAACGC TTATTACAGG 
TGTGTCCTTG GGGAGTTGCG CAAGCAGTAC TGCGCTGGTG GGCTTCATTG GAACAAGGAA 
CGCAAAATTT GTGATTGGCC AAAATCGGCC AAATGCGAGG AGAAAAAACC TGGTCATAAA 
CCTAGCACTT CGTCGTGGCA AAAACCAACA AAACCATCGT ACCGCCCCCC AAGTACCACC 
AACCATTGGC AAACCAAAAC CACGACTAGT ACCACGACCA GACCCACAAC AACAGTATCA 
CAACTAATTG ATGATAAATG TGATTCGGGC CAGTACTACC CTCACGAGTC ATGCTCAAGC 
TTTTATGTTT GTGTTAATGG GCACTTGGTA CCGCAGAATT GTGCCCCAGG GCTTCACTAT 
AATCCGGAAG AACACATGTG TGACTGGAAG TACAAAGTCA AGTGTGTTGG GCGCAAACAA 
TTGGCCCAGA AGTACCAACT CCCCAAAATG GGGGGACCAC AACCCTACTC CGCCTGCAGC 
GAAAATGCCT TTGCCGCATA CCCCGGGGAT TGTACCCGAT ATTTGCACTG TCTTTGGGGC 
AAATATGAAG TGTTTAACTG TGCCCCTGGC TTACACTGGG ATAATAATAA AAATATTTGT 
GATTGGCCTG AGAAAGCAAC CTGTGATGGT ACCAGCAACG TCAATGTTGT TGATATCGTG 
ACAACAGCCA AACCCGCACA AAGTACGACA AGTGTGTCAA CTACTACAAG TTGGAATCCG 
GGCAGTACCA CCAATTACCC GGAATGGCAA CCCACGGAGT GGCACCCTCC CATCCCCCCC 
ACGTCCGAAA AACCGCCCCT TCCCGAAGAA TTAAAACCAC AATCCGGGTA CTTTAAGATA 
GTTTGTTATT TCACAAACTG GGCGTGGTAC CGCAAAGGTT TAGGAAAGTA CCTCCCGGAG 
GATATCGACC CAGACCTCTG CACCCACATC GTTTACGGTT TCGCCGTCTT GGACTTTGAA 
AATTTGATAG TGAAAGCGCA CGACTCGTGG GCCGACTTTG ATAACCAATT CTACAAACGC 
GTCACTGGAT ACAAAGCCAA GGGAATCAAA GTTTCCCTTG CCCTAGGCGG CTGGAACGAC 
TCGCAAGGCG ATAAGTACTC CCGCCTGGTC AATAATCCCG CCGCACGTGC CAGGTTTATC 
AAACACGTGT TGCAATTTTT GGAAAAATGG AACTTTGACG GGCTTGATTT GGACTGGGAG 
TACCCGAAAT GCTGGCAAGT GGATTGCAAG AAAGGCCCAG ATTCGGACAA ACAAGCCTTT 
GCTGCGTGGG TCACCGAACT GAAGCAGGCC TTCAAACCGA AGGGATATCT CCTCTCAGCT 
GCGGTTTCCC CAAGTAAGAC TGTGATAGAT GCAGGGTATG ATGTTCCAGT ACTTGCGCAA 
AACCTGGACT GGGTGGCTGT GATGACGTAC GACTTCCATG GGCAGTGGGA CAAGCAGACG 
GGTCACGTGG CCCCGCTGTA CTACCATCCC GAGGATGAGG TTGTCTTCTA CAACGCGAAC 
TTCTCCATTA ATTACTGGAT CTCGGAAGGA GTACCGCGGA GGAAAATCGT GATGGGCATG 
CCCCTCTACG GCCAGTCCTT CCGCTTGGAG AAGCCCGAAA ACCACGGTTT GAACGCCAAA 
GCCCCAGGTC CAGGCCAAGC CGGGGAATAC ACCCGAGCTG CCGGTTTCCT GGCTTATTAC 
GAAATCTGCC ACAATATCAA AACACAAGGC TGGACTGTGG TACAAGACCC CAATCGACGC 
ATGGGTCCCT ACGCGTACAA AGGGGACCAA TGGGTGTCGT TCGACGATAG GGAGATGATC 
AAGCGCAAAT CGGAGTTTAT CAGGAAGATG GATTTAGGTG GTGGGATGAT CTGGGCCCTT 
GACTTGGACG ATTTTAAGAA CCGGTGTGGT GAAGGCCGAC ATCCCCTTCT GACCACCATT 
AGGAACGTCC TTGCGGATGC TGGAACCGGG CAACAGGAGA CAATTCCGCC CATTGATGAG 
GAACCACCAA GTGTTGAAGC CGTGGAAGAG GAACGTGAAG AAGCACCTCC AAGTGTGCAC 
TCTTCAACGG TGGTACCACC ACCGCAACAT TCCACAACGC AAAGTCTAGT CGATCCAAAA 
TCCGAGTTCA AGGTGGTTTG TTATTTCACG AATTGGGCGT GGTACCGGCA AGGTGTCGGG 
AAGTACCTCC CTTCCGATAT AGACCCAGAC TTGTGTACCC ACATCGTTTA TGGATTTGCG 
GTACTCAACG GCGATCAATT GATCATCAAA CCGCACGACA CTTGGGCCGA TTTCGACAAT 
AAGTTCTACG AAAAGGTCAC AGCCTACAAA TCGAAGGGAA TCAAGGTACT TGTCGCAATC 
GGAGGCTGGA ACGACTCGGC AGGTGACAAG TACTCGCGTC TGGTGAACAA CCCATCGGCC 
CGCAGACGTT TCATAGCCCA CGTTGTCGAT TTCATCGAAA CCAACAACTT TGACGGTTTG 
GACCTAGACT GGGAGTACCC CAAGTGTTGG CAAGTCGATT GTAACAAAGG CCCGGCGTCT 
GACAAGTCAG CGTTTGCCGA CTTTGTCAAA GAATTGCACG AAGCCTTCAA ACCCAAAGGG 
TGGCTCCTAT CAGCTGCAGT TTCGCCAAGT CGGCGCGTGG TAGATGCAGG GTACGATGTA 
CCAACCTTGT CCAAGTACCT GGACTGGATT GCCGTCATGT GCTACGATTA CCATGGGCAA 
TGGGACAAGA TTACGGGGCA CGTGGCGCCC ATGTACGCCC ATCCCGAGGA CGCGGACGCC 
ACTTTCAATA CTAATTTCAC TATTCACTAC TGGATTGAGA AAGGCGCTGA TCGGAGGAAA 
CTCGTGATGG GCATGCCCAT GTATGGACAG TCGTTTTCAT TGGCTGACAA CAACCAAAAT 
GGGCTCAATG CAGCCACCTA TGGGGGCGGA GAGGCCGGGG AGGAAACACG AGCTCGTGGT 
TTCCTAGCCT ATTATGAAAT TTGTACGAAC GTTATTAAGA AAGGGTGGAA GGTGGTACGT 
GACCGCCGGG GACGTATTGG TCCCTACGCC TACCTACGTG ACCAATGGGT CTCCTTCGAC 
GATATAGGCA TGATAAGACA CAAGTCTGAG TTTATCAAGG CCATGGGTCT TGGTGGCGGT 
ATGATCTGGG CGTTGGATTT GGACGATTTT AAAAACCTTT GTGGGTGTGA GGAGTACCCA 
CTACTTCGCA CCATCAACCG GGTGTTACGA GGGTACGCTA AACCTGACCC TAAGTGTATC 
CTAGGCCGTG ATAACGACAA AACTAAACCT AAACCTACAA CAATGAAACC AACCACAACC 
ATTGGTTATG AACCACAAGA ACCGGTACTT CCGGCCGAAG TGGTACCGTG TCAAGGCCGG 
CTTTTCGTAG CCGATGATAC CAACTGTGCC CAGTACTATT TGTGTAACCA GGGCCAGCTC 
CAATTGCAAG TGTGCCCCAA TGGTTTGTTC TGGAATAAGG ACCATTGTGA CTGGCCTGAG 
AACACGGAGT GTCACCCGGA CGCGAGCAGT ACCATGGCAC CATCAACCAC ACCGATGGTA 
CCGGACAAGC CGGTCACAAC CACCACGTCC AGACCCATTG GTACTGACAC CAGCGACGAT 
TATAAGGTCG TTTGTTACTT TACCAACTGG GCGTGGTACC GCCAAGGCGA TGGGAAGTAC 
CTCCCGTCTG ACATAGACCC CTCACTTTGT ACTCACATTA ATTACGGTTT TGCGGTCCTG 
GACTCGTCGA GTATGACCTT AAAACCGCAC GATTCTTGGG CCGATATCGA CAACGAGTTT 
TACAAAAAAG TCGTTTCGTA CAAATCGCGC GGTGTTAAGG TCTTGATAGC CTTAGGCGGT 
TGGAACGACT CAGCCGGTAA CAAATACTCG CGCCTAGTCA ACGACCCACA AGCCCGTGCG 
GCCTTCATAG CCCACGTTCT GGCCTTTATC GAAGAGTGGA ACTTCGATGG CCTTGATTTA 
GACTGGGAGT ACCCCAAATG TTGGCAAGTG GACTGCAACA AAGGCCCAGA CTCGGACAAA 
GAAGCCTTCG CGGCCTTTGT CCGCGAACTG AGCGCGGCCT TCAAGCCCAA AAACCTCCTA 
CTATCAGCTG CGGTTTCGCC CAGCAAGGCA GTGATGGACG CTGGGTACGA CGTGCCAGCC 
TTATCCCAGT ACTTGGACTG GATCGCCGTC ATGACCTACG ATTTCCACGG CCACTGGGAC 
AAACAAACCG GACATGTGGC ACCCTTGTAT TACTACCCTG GCGATGTTTA CGATTATTTC 
AACGCCAACT TTTCGCTAAA CTACTGGATC GAGAAGGGGG CGGACCCAGG AAAAATCGTG 
ATGGGCATGC CCCTCTACGG CCAAAGTTTC AGTCTGGCTG ACGCGGCCGA TAGGGGCCTT 
AACGCCAAGT CGTACGGCCC TGGAGAAGCC GGGGAGTTTA CCCGAGCTGG TGGTTTCCTA 
GCCTACTATG AGATTTGTGA ACGGGTGAAG AAGAGGGGTT GGACGGTGAC GAGGGACCCT 
CAAGGTCGCA TTGGTCCATA TGCCTATTTG TCGAACCAAT GGGTGTCGTA CGATGATATC 
GCCGAAATCC GGCGCAAGTC GCGATTGGTC AAGGCGTTGA AACTGGGCGG GGGCATGGTC 
TGGGCTTTGG ATTTGGACGA TTTTAAGAAC AGGTGTGGGT GTGGCAAACA CCCGCTCTTG 
AAGACTTTAA ACAATGAGTT GAGAGGGACG CCCTCTGATA TCAGCGTGAC TAATTGTACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036067.1
CDS	1..8103
Translation	MELLHITLCAFLILTPTDQRVVTRTIPSFLRDDVERYPSGPELSDRSAAYTNRELYGTRALPLRSAVEHIPDLIADSRRLPLRDAVEKRPPPDEPIDDYEPSFDLFYYPSRQTLYTPVQYFKDLVAEAYVSPFRIDNESQELLKYPSFARGRSLYDSRGRHSEIVETVYDPREDDRVVCYVQAAARYRKEPLAFSPEDLDPFACTHVIYAFATIDPHNFNMISNDDESDIIQGGYISVTGLKRVNPKLKVLISVGEGRDGSHRFSTMVSSANRRREFIRSAITFIKQYNFDGMDIHWEYPGAEKMGGQLSDKEYLNLFLEELSEIFKPRGWVLTIAVPASRFRVEDGFNPQRLGSLVDFINLQAFDFHREREPVADHHANLYSRPGDSGLDLFLSVDYAVKFWTKKGFPKSKIVLGVPFFGRSFTLQFTNETQVGAPIKGPGREGFYTQNPGFLAYFEICDMLLNEGWHKGMDESGSPYMVYGDQWVGYDDSESVEKKVNYVQEMNLGGVFIWATDLDDFKGVCGMKWPLLSTINRHLRGNELLPMQQSQNPPTKPYGTCRSEGIFSDPKNCAAYYVCRSGLSYHLSCAENMMFDPMSGKCEYSLGEKCRPGQIIQVANSLRQYEDLLEYEAKEEGPKVVCYMTNWAFYRKAEGKFVPEHIDQRLCTHVVYAFASLDPEKLLLKEFDPWADLDNNLYERVTSLKDTKAILSLGGWTDSAGDKYSRLVGDGSARRRFVVAVVGFLRRHNFKGLHLDWNYPVCWQSNCKKGASSDKPNFTKLIQVVPTMELRREFDKQKPPLILAAAISGYKEVIDVAYDLPALGTVLDFMSVMTYDYHGAWERQTGHNFTMEYLVSRGAPRGKLLVGVPFYGQSFTLTKGNNHDQGVPSAGPGEAGEYTKQPGMLAYYEICNRIRNQRWIVNRDTSGATGPFAYHGDQWVGFEDIKSVRDKAEYIKTKGFGGAVAWTIDLDDFSNRCCGGSFPLLRSLNRGLGLIPDNPSREDCTKPPEPVTPAPPQVTTGVDTGASSTEHMHPDWTTKPSTWWSSTTTSPWWTTTTTRRTTTTRPTTTSTTTRPTTTNWPTQGTTIPPPAVVMPEVDKPSQPCEPGQYVPDPHNCNAYYRCVLGELRKQYCAGGLHWNKERKICDWPKSAKCEEKKPGHKPSTSSWQKPTKPSYRPPSTTNHWQTKTTTSTTTRPTTTVSQLIDDKCDSGQYYPHESCSSFYVCVNGHLVPQNCAPGLHYNPEEHMCDWKYKVKCVGRKQLAQKYQLPKMGGPQPYSACSENAFAAYPGDCTRYLHCLWGKYEVFNCAPGLHWDNNKNICDWPEKATCDGTSNVNVVDIVTTAKPAQSTTSVSTTTSWNPGSTTNYPEWQPTEWHPPIPPTSEKPPLPEELKPQSGYFKIVCYFTNWAWYRKGLGKYLPEDIDPDLCTHIVYGFAVLDFENLIVKAHDSWADFDNQFYKRVTGYKAKGIKVSLALGGWNDSQGDKYSRLVNNPAARARFIKHVLQFLEKWNFDGLDLDWEYPKCWQVDCKKGPDSDKQAFAAWVTELKQAFKPKGYLLSAAVSPSKTVIDAGYDVPVLAQNLDWVAVMTYDFHGQWDKQTGHVAPLYYHPEDEVVFYNANFSINYWISEGVPRRKIVMGMPLYGQSFRLEKPENHGLNAKAPGPGQAGEYTRAAGFLAYYEICHNIKTQGWTVVQDPNRRMGPYAYKGDQWVSFDDREMIKRKSEFIRKMDLGGGMIWALDLDDFKNRCGEGRHPLLTTIRNVLADAGTGQQETIPPIDEEPPSVEAVEEEREEAPPSVHSSTVVPPPQHSTTQSLVDPKSEFKVVCYFTNWAWYRQGVGKYLPSDIDPDLCTHIVYGFAVLNGDQLIIKPHDTWADFDNKFYEKVTAYKSKGIKVLVAIGGWNDSAGDKYSRLVNNPSARRRFIAHVVDFIETNNFDGLDLDWEYPKCWQVDCNKGPASDKSAFADFVKELHEAFKPKGWLLSAAVSPSRRVVDAGYDVPTLSKYLDWIAVMCYDYHGQWDKITGHVAPMYAHPEDADATFNTNFTIHYWIEKGADRRKLVMGMPMYGQSFSLADNNQNGLNAATYGGGEAGEETRARGFLAYYEICTNVIKKGWKVVRDRRGRIGPYAYLRDQWVSFDDIGMIRHKSEFIKAMGLGGGMIWALDLDDFKNLCGCEEYPLLRTINRVLRGYAKPDPKCILGRDNDKTKPKPTTMKPTTTIGYEPQEPVLPAEVVPCQGRLFVADDTNCAQYYLCNQGQLQLQVCPNGLFWNKDHCDWPENTECHPDASSTMAPSTTPMVPDKPVTTTTSRPIGTDTSDDYKVVCYFTNWAWYRQGDGKYLPSDIDPSLCTHINYGFAVLDSSSMTLKPHDSWADIDNEFYKKVVSYKSRGVKVLIALGGWNDSAGNKYSRLVNDPQARAAFIAHVLAFIEEWNFDGLDLDWEYPKCWQVDCNKGPDSDKEAFAAFVRELSAAFKPKNLLLSAAVSPSKAVMDAGYDVPALSQYLDWIAVMTYDFHGHWDKQTGHVAPLYYYPGDVYDYFNANFSLNYWIEKGADPGKIVMGMPLYGQSFSLADAADRGLNAKSYGPGEAGEFTRAGGFLAYYEICERVKKRGWTVTRDPQGRIGPYAYLSNQWVSYDDIAEIRRKSRLVKALKLGGGMVWALDLDDFKNRCGCGKHPLLKTLNNELRGTPSDISVTNCT

Target ORF information:

RefSeq Version	NM_001042602.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum chitinase 10 (Cht10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042602.1

ORF Insert Sequence:

ATGGAATTAC TGCACATAAC TCTATGTGCG TTCTTAATCC TCACACCGAC CGACCAAAGG 
GTCGTCACAA GGACAATCCC GAGCTTCCTT CGGGACGACG TCGAGCGATA TCCCTCAGGA 
CCCGAGCTTT CGGACAGGAG CGCCGCTTAC ACAAATCGGG AACTGTACGG GACGCGGGCT 
TTGCCGCTCC GTTCGGCGGT TGAACACATC CCGGATTTGA TTGCGGACTC GCGCCGGCTG 
CCACTCAGGG ACGCGGTGGA GAAACGCCCA CCGCCCGACG AACCAATCGA CGATTACGAA 
CCGAGCTTTG ATCTGTTCTA CTACCCGTCC CGACAAACTC TCTACACCCC AGTCCAGTAT 
TTCAAGGATT TGGTGGCCGA GGCGTACGTT TCCCCCTTTC GCATTGACAA CGAATCGCAG 
GAACTGCTAA AGTACCCCTC CTTTGCCCGG GGGCGGAGTT TGTATGACTC CAGAGGGAGG 
CACTCAGAGA TAGTTGAGAC GGTTTATGAC CCACGGGAGG ATGACCGTGT TGTTTGCTAC 
GTCCAAGCGG CTGCCAGGTA CCGGAAGGAG CCCTTGGCCT TCTCCCCCGA GGATTTGGAC 
CCCTTTGCTT GCACCCATGT CATTTATGCG TTCGCGACCA TTGACCCCCA CAATTTTAAC 
ATGATTTCGA ACGACGACGA GTCTGATATT ATCCAAGGGG GCTATATTTC CGTCACGGGA 
CTCAAAAGAG TTAACCCGAA ACTAAAAGTA CTGATCTCGG TTGGTGAGGG ACGGGATGGT 
TCCCACAGGT TCAGTACCAT GGTCTCCAGT GCCAACAGAC GAAGGGAGTT CATCCGGTCC 
GCCATCACTT TCATCAAGCA GTACAACTTT GACGGAATGG ACATCCACTG GGAGTACCCA 
GGGGCCGAGA AAATGGGCGG GCAACTGAGC GATAAGGAGT ACCTGAACCT CTTCCTCGAG 
GAGTTATCTG AAATTTTCAA ACCCAGAGGT TGGGTACTTA CAATTGCGGT ACCAGCCTCC 
CGGTTCCGGG TCGAAGATGG GTTTAACCCC CAACGCTTGG GGTCCCTTGT CGATTTTATC 
AACCTCCAAG CGTTTGATTT CCACCGGGAG CGGGAACCGG TGGCCGACCA CCACGCAAAT 
TTGTACTCAA GGCCGGGCGA TAGTGGCCTT GACCTGTTCC TAAGTGTGGA CTATGCTGTG 
AAGTTCTGGA CCAAGAAGGG GTTCCCCAAG TCCAAGATCG TGCTCGGGGT ACCATTTTTC 
GGGCGTTCGT TCACCCTCCA GTTCACAAAC GAGACGCAAG TTGGGGCCCC GATTAAAGGT 
CCAGGACGGG AAGGGTTCTA TACGCAAAAT CCAGGCTTCC TGGCATATTT CGAAATCTGC 
GATATGTTAC TGAATGAAGG GTGGCACAAA GGGATGGACG AGTCAGGGTC GCCTTACATG 
GTATATGGGG ACCAATGGGT GGGTTATGAC GATAGCGAAA GCGTCGAAAA GAAGGTTAAT 
TACGTGCAAG AGATGAACCT CGGGGGTGTG TTTATTTGGG CTACGGATCT GGACGATTTT 
AAGGGCGTTT GCGGAATGAA ATGGCCCTTG CTGAGTACCA TTAACCGGCA TTTGAGGGGA 
AACGAGTTGT TACCGATGCA ACAAAGCCAA AACCCTCCCA CTAAACCATA TGGTACTTGC 
CGCTCAGAAG GGATCTTCAG TGACCCCAAG AACTGTGCAG CTTATTACGT TTGTCGCTCA 
GGGTTGAGCT ACCACTTGTC GTGTGCCGAG AACATGATGT TTGACCCAAT GTCCGGGAAA 
TGTGAGTACA GCCTGGGGGA GAAGTGTAGG CCGGGTCAGA TCATCCAAGT CGCTAACTCT 
CTAAGACAAT ATGAGGACCT CCTGGAGTAT GAAGCTAAAG AGGAAGGACC AAAAGTGGTG 
TGCTACATGA CCAACTGGGC CTTCTACCGA AAAGCCGAAG GCAAATTCGT CCCGGAACAC 
ATCGACCAAA GGCTTTGCAC ACACGTGGTG TACGCCTTCG CGAGTCTCGA CCCCGAAAAA 
CTCCTCCTGA AGGAGTTCGA TCCTTGGGCC GACTTGGACA ACAATTTGTA CGAAAGAGTC 
ACCTCTTTGA AGGACACCAA GGCGATTTTA TCTCTTGGAG GCTGGACTGA CTCAGCTGGA 
GACAAGTACT CACGTCTTGT GGGCGATGGT TCCGCCAGAC GCCGGTTTGT TGTGGCAGTT 
GTGGGGTTTT TGCGACGACA CAATTTCAAA GGTTTGCATT TGGATTGGAA CTACCCCGTT 
TGTTGGCAAA GCAATTGCAA AAAAGGGGCG TCCTCCGATA AACCCAACTT CACCAAATTG 
ATTCAGGTAG TACCAACTAT GGAGTTGCGG CGCGAGTTCG ACAAACAGAA ACCTCCCCTG 
ATCCTGGCTG CGGCCATTTC GGGGTATAAG GAGGTGATAG ACGTGGCGTA CGACCTACCA 
GCGCTTGGTA CCGTCCTTGA TTTCATGTCA GTGATGACTT ATGACTACCA TGGGGCTTGG 
GAACGCCAAA CGGGGCACAA CTTCACCATG GAGTATTTGG TGTCTCGTGG TGCTCCCCGT 
GGGAAACTGC TAGTCGGAGT ACCATTCTAT GGTCAAAGTT TTACCCTAAC CAAGGGCAAC 
AACCACGACC AGGGAGTCCC AAGTGCCGGT CCCGGCGAAG CCGGTGAATA CACCAAACAG 
CCAGGAATGT TGGCCTATTA CGAAATTTGT AACCGGATCC GGAACCAACG CTGGATCGTC 
AATCGGGACA CTTCGGGTGC CACCGGCCCA TTTGCCTACC ATGGGGACCA ATGGGTCGGT 
TTCGAGGACA TCAAGTCGGT CCGAGACAAG GCCGAATACA TCAAAACCAA AGGTTTTGGC 
GGCGCCGTGG CCTGGACTAT CGACTTGGAC GACTTCTCCA ACCGTTGTTG CGGTGGTTCC 
TTCCCTTTAC TTCGAAGTTT GAACCGTGGC TTGGGATTAA TCCCCGATAA TCCCTCACGA 
GAGGACTGCA CCAAACCACC GGAACCCGTA ACTCCAGCTC CTCCACAAGT CACAACTGGG 
GTTGATACGG GAGCTTCAAG TACCGAACAC ATGCATCCAG ACTGGACGAC CAAACCCAGC 
ACCTGGTGGT CGAGTACTAC GACAAGTCCG TGGTGGACCA CTACCACAAC GAGGAGGACC 
ACTACGACTC GACCTACCAC GACTAGTACC ACGACAAGGC CAACTACGAC CAATTGGCCG 
ACCCAAGGAA CCACAATCCC GCCCCCGGCT GTTGTAATGC CAGAAGTGGA TAAACCATCA 
CAACCATGCG AACCTGGGCA ATACGTACCA GACCCACACA ATTGTAACGC TTATTACAGG 
TGTGTCCTTG GGGAGTTGCG CAAGCAGTAC TGCGCTGGTG GGCTTCATTG GAACAAGGAA 
CGCAAAATTT GTGATTGGCC AAAATCGGCC AAATGCGAGG AGAAAAAACC TGGTCATAAA 
CCTAGCACTT CGTCGTGGCA AAAACCAACA AAACCATCGT ACCGCCCCCC AAGTACCACC 
AACCATTGGC AAACCAAAAC CACGACTAGT ACCACGACCA GACCCACAAC AACAGTATCA 
CAACTAATTG ATGATAAATG TGATTCGGGC CAGTACTACC CTCACGAGTC ATGCTCAAGC 
TTTTATGTTT GTGTTAATGG GCACTTGGTA CCGCAGAATT GTGCCCCAGG GCTTCACTAT 
AATCCGGAAG AACACATGTG TGACTGGAAG TACAAAGTCA AGTGTGTTGG GCGCAAACAA 
TTGGCCCAGA AGTACCAACT CCCCAAAATG GGGGGACCAC AACCCTACTC CGCCTGCAGC 
GAAAATGCCT TTGCCGCATA CCCCGGGGAT TGTACCCGAT ATTTGCACTG TCTTTGGGGC 
AAATATGAAG TGTTTAACTG TGCCCCTGGC TTACACTGGG ATAATAATAA AAATATTTGT 
GATTGGCCTG AGAAAGCAAC CTGTGATGGT ACCAGCAACG TCAATGTTGT TGATATCGTG 
ACAACAGCCA AACCCGCACA AAGTACGACA AGTGTGTCAA CTACTACAAG TTGGAATCCG 
GGCAGTACCA CCAATTACCC GGAATGGCAA CCCACGGAGT GGCACCCTCC CATCCCCCCC 
ACGTCCGAAA AACCGCCCCT TCCCGAAGAA TTAAAACCAC AATCCGGGTA CTTTAAGATA 
GTTTGTTATT TCACAAACTG GGCGTGGTAC CGCAAAGGTT TAGGAAAGTA CCTCCCGGAG 
GATATCGACC CAGACCTCTG CACCCACATC GTTTACGGTT TCGCCGTCTT GGACTTTGAA 
AATTTGATAG TGAAAGCGCA CGACTCGTGG GCCGACTTTG ATAACCAATT CTACAAACGC 
GTCACTGGAT ACAAAGCCAA GGGAATCAAA GTTTCCCTTG CCCTAGGCGG CTGGAACGAC 
TCGCAAGGCG ATAAGTACTC CCGCCTGGTC AATAATCCCG CCGCACGTGC CAGGTTTATC 
AAACACGTGT TGCAATTTTT GGAAAAATGG AACTTTGACG GGCTTGATTT GGACTGGGAG 
TACCCGAAAT GCTGGCAAGT GGATTGCAAG AAAGGCCCAG ATTCGGACAA ACAAGCCTTT 
GCTGCGTGGG TCACCGAACT GAAGCAGGCC TTCAAACCGA AGGGATATCT CCTCTCAGCT 
GCGGTTTCCC CAAGTAAGAC TGTGATAGAT GCAGGGTATG ATGTTCCAGT ACTTGCGCAA 
AACCTGGACT GGGTGGCTGT GATGACGTAC GACTTCCATG GGCAGTGGGA CAAGCAGACG 
GGTCACGTGG CCCCGCTGTA CTACCATCCC GAGGATGAGG TTGTCTTCTA CAACGCGAAC 
TTCTCCATTA ATTACTGGAT CTCGGAAGGA GTACCGCGGA GGAAAATCGT GATGGGCATG 
CCCCTCTACG GCCAGTCCTT CCGCTTGGAG AAGCCCGAAA ACCACGGTTT GAACGCCAAA 
GCCCCAGGTC CAGGCCAAGC CGGGGAATAC ACCCGAGCTG CCGGTTTCCT GGCTTATTAC 
GAAATCTGCC ACAATATCAA AACACAAGGC TGGACTGTGG TACAAGACCC CAATCGACGC 
ATGGGTCCCT ACGCGTACAA AGGGGACCAA TGGGTGTCGT TCGACGATAG GGAGATGATC 
AAGCGCAAAT CGGAGTTTAT CAGGAAGATG GATTTAGGTG GTGGGATGAT CTGGGCCCTT 
GACTTGGACG ATTTTAAGAA CCGGTGTGGT GAAGGCCGAC ATCCCCTTCT GACCACCATT 
AGGAACGTCC TTGCGGATGC TGGAACCGGG CAACAGGAGA CAATTCCGCC CATTGATGAG 
GAACCACCAA GTGTTGAAGC CGTGGAAGAG GAACGTGAAG AAGCACCTCC AAGTGTGCAC 
TCTTCAACGG TGGTACCACC ACCGCAACAT TCCACAACGC AAAGTCTAGT CGATCCAAAA 
TCCGAGTTCA AGGTGGTTTG TTATTTCACG AATTGGGCGT GGTACCGGCA AGGTGTCGGG 
AAGTACCTCC CTTCCGATAT AGACCCAGAC TTGTGTACCC ACATCGTTTA TGGATTTGCG 
GTACTCAACG GCGATCAATT GATCATCAAA CCGCACGACA CTTGGGCCGA TTTCGACAAT 
AAGTTCTACG AAAAGGTCAC AGCCTACAAA TCGAAGGGAA TCAAGGTACT TGTCGCAATC 
GGAGGCTGGA ACGACTCGGC AGGTGACAAG TACTCGCGTC TGGTGAACAA CCCATCGGCC 
CGCAGACGTT TCATAGCCCA CGTTGTCGAT TTCATCGAAA CCAACAACTT TGACGGTTTG 
GACCTAGACT GGGAGTACCC CAAGTGTTGG CAAGTCGATT GTAACAAAGG CCCGGCGTCT 
GACAAGTCAG CGTTTGCCGA CTTTGTCAAA GAATTGCACG AAGCCTTCAA ACCCAAAGGG 
TGGCTCCTAT CAGCTGCAGT TTCGCCAAGT CGGCGCGTGG TAGATGCAGG GTACGATGTA 
CCAACCTTGT CCAAGTACCT GGACTGGATT GCCGTCATGT GCTACGATTA CCATGGGCAA 
TGGGACAAGA TTACGGGGCA CGTGGCGCCC ATGTACGCCC ATCCCGAGGA CGCGGACGCC 
ACTTTCAATA CTAATTTCAC TATTCACTAC TGGATTGAGA AAGGCGCTGA TCGGAGGAAA 
CTCGTGATGG GCATGCCCAT GTATGGACAG TCGTTTTCAT TGGCTGACAA CAACCAAAAT 
GGGCTCAATG CAGCCACCTA TGGGGGCGGA GAGGCCGGGG AGGAAACACG AGCTCGTGGT 
TTCCTAGCCT ATTATGAAAT TTGTACGAAC GTTATTAAGA AAGGGTGGAA GGTGGTACGT 
GACCGCCGGG GACGTATTGG TCCCTACGCC TACCTACGTG ACCAATGGGT CTCCTTCGAC 
GATATAGGCA TGATAAGACA CAAGTCTGAG TTTATCAAGG CCATGGGTCT TGGTGGCGGT 
ATGATCTGGG CGTTGGATTT GGACGATTTT AAAAACCTTT GTGGGTGTGA GGAGTACCCA 
CTACTTCGCA CCATCAACCG GGTGTTACGA GGGTACGCTA AACCTGACCC TAAGTGTATC 
CTAGGCCGTG ATAACGACAA AACTAAACCT AAACCTACAA CAATGAAACC AACCACAACC 
ATTGGTTATG AACCACAAGA ACCGGTACTT CCGGCCGAAG TGGTACCGTG TCAAGGCCGG 
CTTTTCGTAG CCGATGATAC CAACTGTGCC CAGTACTATT TGTGTAACCA GGGCCAGCTC 
CAATTGCAAG TGTGCCCCAA TGGTTTGTTC TGGAATAAGG ACCATTGTGA CTGGCCTGAG 
AACACGGAGT GTCACCCGGA CGCGAGCAGT ACCATGGCAC CATCAACCAC ACCGATGGTA 
CCGGACAAGC CGGTCACAAC CACCACGTCC AGACCCATTG GTACTGACAC CAGCGACGAT 
TATAAGGTCG TTTGTTACTT TACCAACTGG GCGTGGTACC GCCAAGGCGA TGGGAAGTAC 
CTCCCGTCTG ACATAGACCC CTCACTTTGT ACTCACATTA ATTACGGTTT TGCGGTCCTG 
GACTCGTCGA GTATGACCTT AAAACCGCAC GATTCTTGGG CCGATATCGA CAACGAGTTT 
TACAAAAAAG TCGTTTCGTA CAAATCGCGC GGTGTTAAGG TCTTGATAGC CTTAGGCGGT 
TGGAACGACT CAGCCGGTAA CAAATACTCG CGCCTAGTCA ACGACCCACA AGCCCGTGCG 
GCCTTCATAG CCCACGTTCT GGCCTTTATC GAAGAGTGGA ACTTCGATGG CCTTGATTTA 
GACTGGGAGT ACCCCAAATG TTGGCAAGTG GACTGCAACA AAGGCCCAGA CTCGGACAAA 
GAAGCCTTCG CGGCCTTTGT CCGCGAACTG AGCGCGGCCT TCAAGCCCAA AAACCTCCTA 
CTATCAGCTG CGGTTTCGCC CAGCAAGGCA GTGATGGACG CTGGGTACGA CGTGCCAGCC 
TTATCCCAGT ACTTGGACTG GATCGCCGTC ATGACCTACG ATTTCCACGG CCACTGGGAC 
AAACAAACCG GACATGTGGC ACCCTTGTAT TACTACCCTG GCGATGTTTA CGATTATTTC 
AACGCCAACT TTTCGCTAAA CTACTGGATC GAGAAGGGGG CGGACCCAGG AAAAATCGTG 
ATGGGCATGC CCCTCTACGG CCAAAGTTTC AGTCTGGCTG ACGCGGCCGA TAGGGGCCTT 
AACGCCAAGT CGTACGGCCC TGGAGAAGCC GGGGAGTTTA CCCGAGCTGG TGGTTTCCTA 
GCCTACTATG AGATTTGTGA ACGGGTGAAG AAGAGGGGTT GGACGGTGAC GAGGGACCCT 
CAAGGTCGCA TTGGTCCATA TGCCTATTTG TCGAACCAAT GGGTGTCGTA CGATGATATC 
GCCGAAATCC GGCGCAAGTC GCGATTGGTC AAGGCGTTGA AACTGGGCGG GGGCATGGTC 
TGGGCTTTGG ATTTGGACGA TTTTAAGAAC AGGTGTGGGT GTGGCAAACA CCCGCTCTTG 
AAGACTTTAA ACAATGAGTT GAGAGGGACG CCCTCTGATA TCAGCGTGAC TAATTGTACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cht10 ( XM_008199916.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008198138.1 Tribolium castaneum chitinase 10 (Cht10), transcript variant X1, mRNA.

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CloneID	OTg13332
Clone ID Related Accession (Same CDS sequence)	XM_008199916.2
Accession Version	XM_008199916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	chitinase 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450195.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008199916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAC TGCACATAAC TCTATGTGCG TTCTTAATCC TCACACCGAC CGACCAAAGG 
GTCGTCACAA GGACAATCCC GAGCTTCCTT CGGGACGACG TCGAGCGATA TCCCTCAGGA 
CCCGAGCTTT CGGACAGGAG CGCCGCTTAC ACAAATCGGG AACTGTACGG GACGCGGGCT 
TTGCCGCTCC GTTCGGCGGT TGAACACATC CCGGATTTGA TTGCGGACTC GCGCCGGCTG 
CCACTCAGGG ACGCGGTGGA GAAACGCCCA CCGCCCGACG AACCAATCGA CGATTACGAA 
CCGAGCTTTG ATCTGTTCTA CTACCCGTCC CGACAAACTC TCTACACCCC AGTCCAGTAT 
TTCAAGGATT TGGTGGCCGA GGCGTACGTT TCCCCCTTTC GCATTGACAA CGAATCGCAG 
GAACTGCTAA AGTACCCCTC CTTTGCCCGG GGGCGGAGTT TGTATGACTC CAGAGGGAGG 
CACTCAGAGA TAGTTGAGAC GGTTTATGAC CCACGGGAGG ATGACCGTGT TGTTTGCTAC 
GTCCAAGCGG CTGCCAGGTA CCGGAAGGAG CCCTTGGCCT TCTCCCCCGA GGATTTGGAC 
CCCTTTGCTT GCACCCATGT CATTTATGCG TTCGCGACCA TTGACCCCCA CAATTTTAAC 
ATGATTTCGA ACGACGACGA GTCTGATATT ATCCAAGGGG GCTATATTTC CGTCACGGGA 
CTCAAAAGAG TTAACCCGAA ACTAAAAGTA CTGATCTCGG TTGGTGAGGG ACGGGATGGT 
TCCCACAGGT TCAGTACCAT GGTCTCCAGT GCCAACAGAC GAAGGGAGTT CATCCGGTCC 
GCCATCACTT TCATCAAGCA GTACAACTTT GACGGAATGG ACATCCACTG GGAGTACCCA 
GGGGCCGAGA AAATGGGCGG GCAACTGAGC GATAAGGAGT ACCTGAACCT CTTCCTCGAG 
GAGTTATCTG AAATTTTCAA ACCCAGAGGT TGGGTACTTA CAATTGCGGT ACCAGCCTCC 
CGGTTCCGGG TCGAAGATGG GTTTAACCCC CAACGCTTGG GGTCCCTTGT CGATTTTATC 
AACCTCCAAG CGTTTGATTT CCACCGGGAG CGGGAACCGG TGGCCGACCA CCACGCAAAT 
TTGTACTCAA GGCCGGGCGA TAGTGGCCTT GACCTGTTCC TAAGTGTGGA CTATGCTGTG 
AAGTTCTGGA CCAAGAAGGG GTTCCCCAAG TCCAAGATCG TGCTCGGGGT ACCATTTTTC 
GGGCGTTCGT TCACCCTCCA GTTCACAAAC GAGACGCAAG TTGGGGCCCC GATTAAAGGT 
CCAGGACGGG AAGGGTTCTA TACGCAAAAT CCAGGCTTCC TGGCATATTT CGAAATCTGC 
GATATGTTAC TGAATGAAGG GTGGCACAAA GGGATGGACG AGTCAGGGTC GCCTTACATG 
GTATATGGGG ACCAATGGGT GGGTTATGAC GATAGCGAAA GCGTCGAAAA GAAGGTTAAT 
TACGTGCAAG AGATGAACCT CGGGGGTGTG TTTATTTGGG CTACGGATCT GGACGATTTT 
AAGGGCGTTT GCGGAATGAA ATGGCCCTTG CTGAGTACCA TTAACCGGCA TTTGAGGGGA 
AACGAGTTGT TACCGATGCA ACAAAGCCAA AACCCTCCCA CTAAACCATA TGGTACTTGC 
CGCTCAGAAG GGATCTTCAG TGACCCCAAG AACTGTGCAG CTTATTACGT TTGTCGCTCA 
GGGTTGAGCT ACCACTTGTC GTGTGCCGAG AACATGATGT TTGACCCAAT GTCCGGGAAA 
TGTGAGTACA GCCTGGGGGA GAAGTGTAGG CCGGGTCAGA TCATCCAAGT CGCTAACTCT 
CTAAGACAAT ATGAGGACCT CCTGGAGTAT GAAGCTAAAG AGGAAGGACC AAAAGTGGTG 
TGCTACATGA CCAACTGGGC CTTCTACCGA AAAGCCGAAG GCAAATTCGT CCCGGAACAC 
ATCGACCAAA GGCTTTGCAC ACACGTGGTG TACGCCTTCG CGAGTCTCGA CCCCGAAAAA 
CTCCTCCTGA AGGAGTTCGA TCCTTGGGCC GACTTGGACA ACAATTTGTA CGAAAGAGTC 
ACCTCTTTGA AGGACACCAA GGCGATTTTA TCTCTTGGAG GCTGGACTGA CTCAGCTGGA 
GACAAGTACT CACGTCTTGT GGGCGATGGT TCCGCCAGAC GCCGGTTTGT TGTGGCAGTT 
GTGGGGTTTT TGCGACGACA CAATTTCAAA GGTTTGCATT TGGATTGGAA CTACCCCGTT 
TGTTGGCAAA GCAATTGCAA AAAAGGGGCG TCCTCCGATA AACCCAACTT CACCAAATTG 
ATTCAGGAGT TGCGGCGCGA GTTCGACAAA CAGAAACCTC CCCTGATCCT GGCTGCGGCC 
ATTTCGGGGT ATAAGGAGGT GATAGACGTG GCGTACGACC TACCAGCGCT TGGTACCGTC 
CTTGATTTCA TGTCAGTGAT GACTTATGAC TACCATGGGG CTTGGGAACG CCAAACGGGG 
CACGTGAGTC CGCTGTTTCA CCGGCCTGGG GATAAGTACC CCCAGTACAA CACTAACTTC 
ACCATGGAGT ATTTGGTGTC TCGTGGTGCT CCCCGTGGGA AACTGCTAGT CGGAGTACCA 
TTCTATGGTC AAAGTTTTAC CCTAACCAAG GGCAACAACC ACGACCAGGG AGTCCCAAGT 
GCCGGTCCCG GCGAAGCCGG TGAATACACC AAACAGCCAG GAATGTTGGC CTATTACGAA 
ATTTGTAACC GGATCCGGAA CCAACGCTGG ATCGTCAATC GGGACACTTC GGGTGCCACC 
GGCCCATTTG CCTACCATGG GGACCAATGG GTCGGTTTCG AGGACATCAA GTCGGTCCGA 
GACAAGGCCG AATACATCAA AACCAAAGGT TTTGGCGGCG CCGTGGCCTG GACTATCGAC 
TTGGACGACT TCTCCAACCG TTGTTGCGGT GGTTCCTTCC CTTTACTTCG AAGTTTGAAC 
CGTGGCTTGG GATTAATCCC CGATAATCCC TCACGAGAGG ACTGCACCAA ACCACCGGAA 
CCCGTAACTC CAGCTCCTCC ACAAGTCACA ACTGGGGTTG ATACGGGAGC TTCAAGTACC 
GAACACATGC ATCCAGACTG GACGACCAAA CCCAGCACCT GGTGGTCGAG TACTACGACA 
AGTCCGTGGT GGACCACTAC CACAACGAGG AGGACCACTA CGACTCGACC TACCACGACT 
AGTACCACGA CAAGGCCAAC TACGACCAAT TGGCCGACCC AAGGAACCAC AATCCCGCCC 
CCGGCTGTTG TAATGCCAGA AGTGGATAAA CCATCACAAC CATGCGAACC TGGGCAATAC 
GTACCAGACC CACACAATTG TAACGCTTAT TACAGGTGTG TCCTTGGGGA GTTGCGCAAG 
CAGTACTGCG CTGGTGGGCT TCATTGGAAC AAGGAACGCA AAATTTGTGA TTGGCCAAAA 
TCGGCCAAAT GCGAGGAGAA AAAACCTGGT CATAAACCTA GCACTTCGTC GTGGCAAAAA 
CCAACAAAAC CATCGTACCG CCCCCCAAGT ACCACCAACC ATTGGCAAAC CAAAACCACG 
ACTAGTACCA CGACCAGACC CACAACAACA GTATCACAAC TAATTGATGA TAAATGTGAT 
TCGGGCCAGT ACTACCCTCA CGAGTCATGC TCAAGCTTTT ATGTTTGTGT TAATGGGCAC 
TTGGTACCGC AGAATTGTGC CCCAGGGCTT CACTATAATC CGGAAGAACA CATGTGTGAC 
TGGAAGTACA AAGTCAAGTG TGTTGGGCGC AAACAATTGG CCCAGAAGTA CCAACTCCCC 
AAAATGGGGG GACCACAACC CTACTCCGCC TGCAGCGAAA ATGCCTTTGC CGCATACCCC 
GGGGATTGTA CCCGATATTT GCACTGTCTT TGGGGCAAAT ATGAAGTGTT TAACTGTGCC 
CCTGGCTTAC ACTGGGATAA TAATAAAAAT ATTTGTGATT GGCCTGAGAA AGCAACCTGT 
GATGGTACCA GCAACGTCAA TGTTGTTGAT ATCGTGACAA CAGCCAAACC CGCACAAAGT 
ACGACAAGTG TGTCAACTAC TACAAGTTGG AATCCGGGCA GTACCACCAA TTACCCGGAA 
TGGCAACCCA CGGAGTGGCA CCCTCCCATC CCCCCCACGT CCGAAAAACC GCCCCTTCCC 
GAAGAATTAA AACCACAATC CGGGTACTTT AAGATAGTTT GTTATTTCAC AAACTGGGCG 
TGGTACCGCA AAGGTTTAGG AAAGTACCTC CCGGAGGATA TCGACCCAGA CCTCTGCACC 
CACATCGTTT ACGGTTTCGC CGTCTTGGAC TTTGAAAATT TGATAGTGAA AGCGCACGAC 
TCGTGGGCCG ACTTTGATAA CCAATTCTAC AAACGCGTCA CTGGATACAA AGCCAAGGGA 
ATCAAAGTTT CCCTTGCCCT AGGCGGCTGG AACGACTCGC AAGGCGATAA GTACTCCCGC 
CTGGTCAATA ATCCCGCCGC ACGTGCCAGG TTTATCAAAC ACGTGTTGCA ATTTTTGGAA 
AAATGGAACT TTGACGGGCT TGATTTGGAC TGGGAGTACC CGAAATGCTG GCAAGTGGAT 
TGCAAGAAAG GCCCAGATTC GGACAAACAA GCCTTTGCTG CGTGGGTCAC CGAACTGAAG 
CAGGCCTTCA AACCGAAGGG ATATCTCCTC TCAGCTGCGG TTTCCCCAAG TAAGACTGTG 
ATAGATGCAG GGTATGATGT TCCAGTACTT GCGCAAAACC TGGACTGGGT GGCTGTGATG 
ACGTACGACT TCCATGGGCA GTGGGACAAG CAGACGGGTC ACGTGGCCCC GCTGTACTAC 
CATCCCGAGG ATGAGGTTGT CTTCTACAAC GCGAACTTCT CCATTAATTA CTGGATCTCG 
GAAGGAGTAC CGCGGAGGAA AATCGTGATG GGCATGCCCC TCTACGGCCA GTCCTTCCGC 
TTGGAGAAGC CCGAAAACCA CGGTTTGAAC GCCAAAGCCC CAGGTCCAGG CCAAGCCGGG 
GAATACACCC GAGCTGCCGG TTTCCTGGCT TATTACGAAA TCTGCCACAA TATCAAAACA 
CAAGGCTGGA CTGTGGTACA AGACCCCAAT CGACGCATGG GTCCCTACGC GTACAAAGGG 
GACCAATGGG TGTCGTTCGA CGATAGGGAG ATGATCAAGC GCAAATCGGA GTTTATCAGG 
AAGATGGATT TAGGTGGTGG GATGATCTGG GCCCTTGACT TGGACGATTT TAAGAACCGG 
TGTGGTGAAG GCCGACATCC CCTTCTGACC ACCATTAGGA ACGTCCTTGC GGATGCTGGA 
ACCGGGCAAC AGGAGACAAT TCCGCCCATT GATGAGGAAC CACCAAGTGT TGAAGCCGTG 
GAAGAGGAAC GTGAAGAAGC ACCTCCAAGT GTGCACTCTT CAACGGTGGT ACCACCACCG 
CAACATTCCA CAACGCAAAG TCTAGTCGAT CCAAAATCCG AGTTCAAGGT GGTTTGTTAT 
TTCACGAATT GGGCGTGGTA CCGGCAAGGT GTCGGGAAGT ACCTCCCTTC CGATATAGAC 
CCAGACTTGT GTACCCACAT CGTTTATGGA TTTGCGGTAC TCAACGGCGA TCAATTGATC 
ATCAAACCGC ACGACACTTG GGCCGATTTC GACAATAAGT TCTACGAAAA GGTCACAGCC 
TACAAATCGA AGGGAATCAA GGTACTTGTC GCAATCGGAG GCTGGAACGA CTCGGCAGGT 
GACAAGTACT CGCGTCTGGT GAACAACCCA TCGGCCCGCA GACGTTTCAT AGCCCACGTT 
GTCGATTTCA TCGAAACCAA CAACTTTGAC GGTTTGGACC TAGACTGGGA GTACCCCAAG 
TGTTGGCAAG TCGATTGTAA CAAAGGCCCG GCGTCTGACA AGTCAGCGTT TGCCGACTTT 
GTCAAAGAAT TGCACGAAGC CTTCAAACCC AAAGGGTGGC TCCTATCAGC TGCAGTTTCG 
CCAAGTCGGC GCGTGGTAGA TGCAGGGTAC GATGTACCAA CCTTGTCCAA GTACCTGGAC 
TGGATTGCCG TCATGTGCTA CGATTACCAT GGGCAATGGG ACAAGATTAC GGGGCACGTG 
GCGCCCATGT ACGCCCATCC CGAGGACGCG GACGCCACTT TCAATACTAA TTTCACTATT 
CACTACTGGA TTGAGAAAGG CGCTGATCGG AGGAAACTCG TGATGGGCAT GCCCATGTAT 
GGACAGTCGT TTTCATTGGC TGACAACAAC CAAAATGGGC TCAATGCAGC CACCTATGGG 
GGCGGAGAGG CCGGGGAGGA AACACGAGCT CGTGGTTTCC TAGCCTATTA TGAAATTTGT 
ACGAACGTTA TTAAGAAAGG GTGGAAGGTG GTACGTGACC GCCGGGGACG TATTGGTCCC 
TACGCCTACC TACGTGACCA ATGGGTCTCC TTCGACGATA TAGGCATGAT AAGACACAAG 
TCTGAGTTTA TCAAGGCCAT GGGTCTTGGT GGCGGTATGA TCTGGGCGTT GGATTTGGAC 
GATTTTAAAA ACCTTTGTGG GTGTGAGGAG TACCCACTAC TTCGCACCAT CAACCGGGTG 
TTACGAGGGT ACGCTAAACC TGACCCTAAG TGTATCCTAG GCCGTGATAA CGACAAAACT 
AAACCTAAAC CTACAACAAT GAAACCAACC ACAACCATTG GTTATGAACC ACAAGAACCG 
GTACTTCCGG CCGAAGTGGT ACCGTGTCAA GGCCGGCTTT TCGTAGCCGA TGATACCAAC 
TGTGCCCAGT ACTATTTGTG TAACCAGGGC CAGCTCCAAT TGCAAGTGTG CCCCAATGGT 
TTGTTCTGGA ATAAGGACCA TTGTGACTGG CCTGAGAACA CGGAGTGTCA CCCGGACGCG 
AGCAGTACCA TGGCACCATC AACCACACCG ATGGTACCGG ACAAGCCGGT CACAACCACC 
ACGTCCAGAC CCATTGGTAC TGACACCAGC GACGATTATA AGGTCGTTTG TTACTTTACC 
AACTGGGCGT GGTACCGCCA AGGCGATGGG AAGTACCTCC CGTCTGACAT AGACCCCTCA 
CTTTGTACTC ACATTAATTA CGGTTTTGCG GTCCTGGACT CGTCGAGTAT GACCTTAAAA 
CCGCACGATT CTTGGGCCGA TATCGACAAC GAGTTTTACA AAAAAGTCGT TTCGTACAAA 
TCGCGCGGTG TTAAGGTCTT GATAGCCTTA GGCGGTTGGA ACGACTCAGC CGGTAACAAA 
TACTCGCGCC TAGTCAACGA CCCACAAGCC CGTGCGGCCT TCATAGCCCA CGTTCTGGCC 
TTTATCGAAG AGTGGAACTT CGATGGCCTT GATTTAGACT GGGAGTACCC CAAATGTTGG 
CAAGTGGACT GCAACAAAGG CCCAGACTCG GACAAAGAAG CCTTCGCGGC CTTTGTCCGC 
GAACTGAGCG CGGCCTTCAA GCCCAAAAAC CTCCTACTAT CAGCTGCGGT TTCGCCCAGC 
AAGGCAGTGA TAGACGCTGG GTACGACGTG CCAGCCTTAT CCCAGTACTT GGACTGGATC 
GCCGTCATGA CCTACGATTT CCACGGCCAC TGGGACAAAC AAACCGGACA TGTGGCACCC 
TTGTATTACT ACCCTGGCGA TGTTTACGAT TATTTCAACG CCAACTTTTC GCTAAACTAC 
TGGATCGAGA AAGGGGCGGA CCCAGGAAAA ATCGTGATGG GCATGCCCCT CTACGGCCAA 
AGTTTCAGTC TGGCTGACGC GGCCGATAGG GGCCTTAACG CCAAGTCGTA CGGCCCTGGA 
GAAGCCGGGG AGTTTACCCG AGCTGGTGGT TTCCTAGCCT ACTATGAGAT TTGTGAACGG 
GTGAAGAAGA GGGGTTGGAC GGTGACGAGG GACCCTCAAG GTCGCATTGG TCCATATGCC 
TATTTGTCGA ACCAATGGGT GTCGTACGAT GATATCGCCG AAATCCGGCG CAAGTCGCGA 
TTGGTCAAGG CGTTGAAACT GGGCGGGGGC ATGGTCTGGG CTTTGGATTT GGACGATTTT 
AAGAACAGGT GTGGGTGTGG CAAACACCCG CTCTTGAAGA CTTTAAACAA TGAGTTGAGA 
GGGACGCCCT CTGATATCAG CGTGACTAAT TGTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008198138.1
CDS	210..8348
Translation	MELLHITLCAFLILTPTDQRVVTRTIPSFLRDDVERYPSGPELSDRSAAYTNRELYGTRALPLRSAVEHIPDLIADSRRLPLRDAVEKRPPPDEPIDDYEPSFDLFYYPSRQTLYTPVQYFKDLVAEAYVSPFRIDNESQELLKYPSFARGRSLYDSRGRHSEIVETVYDPREDDRVVCYVQAAARYRKEPLAFSPEDLDPFACTHVIYAFATIDPHNFNMISNDDESDIIQGGYISVTGLKRVNPKLKVLISVGEGRDGSHRFSTMVSSANRRREFIRSAITFIKQYNFDGMDIHWEYPGAEKMGGQLSDKEYLNLFLEELSEIFKPRGWVLTIAVPASRFRVEDGFNPQRLGSLVDFINLQAFDFHREREPVADHHANLYSRPGDSGLDLFLSVDYAVKFWTKKGFPKSKIVLGVPFFGRSFTLQFTNETQVGAPIKGPGREGFYTQNPGFLAYFEICDMLLNEGWHKGMDESGSPYMVYGDQWVGYDDSESVEKKVNYVQEMNLGGVFIWATDLDDFKGVCGMKWPLLSTINRHLRGNELLPMQQSQNPPTKPYGTCRSEGIFSDPKNCAAYYVCRSGLSYHLSCAENMMFDPMSGKCEYSLGEKCRPGQIIQVANSLRQYEDLLEYEAKEEGPKVVCYMTNWAFYRKAEGKFVPEHIDQRLCTHVVYAFASLDPEKLLLKEFDPWADLDNNLYERVTSLKDTKAILSLGGWTDSAGDKYSRLVGDGSARRRFVVAVVGFLRRHNFKGLHLDWNYPVCWQSNCKKGASSDKPNFTKLIQELRREFDKQKPPLILAAAISGYKEVIDVAYDLPALGTVLDFMSVMTYDYHGAWERQTGHVSPLFHRPGDKYPQYNTNFTMEYLVSRGAPRGKLLVGVPFYGQSFTLTKGNNHDQGVPSAGPGEAGEYTKQPGMLAYYEICNRIRNQRWIVNRDTSGATGPFAYHGDQWVGFEDIKSVRDKAEYIKTKGFGGAVAWTIDLDDFSNRCCGGSFPLLRSLNRGLGLIPDNPSREDCTKPPEPVTPAPPQVTTGVDTGASSTEHMHPDWTTKPSTWWSSTTTSPWWTTTTTRRTTTTRPTTTSTTTRPTTTNWPTQGTTIPPPAVVMPEVDKPSQPCEPGQYVPDPHNCNAYYRCVLGELRKQYCAGGLHWNKERKICDWPKSAKCEEKKPGHKPSTSSWQKPTKPSYRPPSTTNHWQTKTTTSTTTRPTTTVSQLIDDKCDSGQYYPHESCSSFYVCVNGHLVPQNCAPGLHYNPEEHMCDWKYKVKCVGRKQLAQKYQLPKMGGPQPYSACSENAFAAYPGDCTRYLHCLWGKYEVFNCAPGLHWDNNKNICDWPEKATCDGTSNVNVVDIVTTAKPAQSTTSVSTTTSWNPGSTTNYPEWQPTEWHPPIPPTSEKPPLPEELKPQSGYFKIVCYFTNWAWYRKGLGKYLPEDIDPDLCTHIVYGFAVLDFENLIVKAHDSWADFDNQFYKRVTGYKAKGIKVSLALGGWNDSQGDKYSRLVNNPAARARFIKHVLQFLEKWNFDGLDLDWEYPKCWQVDCKKGPDSDKQAFAAWVTELKQAFKPKGYLLSAAVSPSKTVIDAGYDVPVLAQNLDWVAVMTYDFHGQWDKQTGHVAPLYYHPEDEVVFYNANFSINYWISEGVPRRKIVMGMPLYGQSFRLEKPENHGLNAKAPGPGQAGEYTRAAGFLAYYEICHNIKTQGWTVVQDPNRRMGPYAYKGDQWVSFDDREMIKRKSEFIRKMDLGGGMIWALDLDDFKNRCGEGRHPLLTTIRNVLADAGTGQQETIPPIDEEPPSVEAVEEEREEAPPSVHSSTVVPPPQHSTTQSLVDPKSEFKVVCYFTNWAWYRQGVGKYLPSDIDPDLCTHIVYGFAVLNGDQLIIKPHDTWADFDNKFYEKVTAYKSKGIKVLVAIGGWNDSAGDKYSRLVNNPSARRRFIAHVVDFIETNNFDGLDLDWEYPKCWQVDCNKGPASDKSAFADFVKELHEAFKPKGWLLSAAVSPSRRVVDAGYDVPTLSKYLDWIAVMCYDYHGQWDKITGHVAPMYAHPEDADATFNTNFTIHYWIEKGADRRKLVMGMPMYGQSFSLADNNQNGLNAATYGGGEAGEETRARGFLAYYEICTNVIKKGWKVVRDRRGRIGPYAYLRDQWVSFDDIGMIRHKSEFIKAMGLGGGMIWALDLDDFKNLCGCEEYPLLRTINRVLRGYAKPDPKCILGRDNDKTKPKPTTMKPTTTIGYEPQEPVLPAEVVPCQGRLFVADDTNCAQYYLCNQGQLQLQVCPNGLFWNKDHCDWPENTECHPDASSTMAPSTTPMVPDKPVTTTTSRPIGTDTSDDYKVVCYFTNWAWYRQGDGKYLPSDIDPSLCTHINYGFAVLDSSSMTLKPHDSWADIDNEFYKKVVSYKSRGVKVLIALGGWNDSAGNKYSRLVNDPQARAAFIAHVLAFIEEWNFDGLDLDWEYPKCWQVDCNKGPDSDKEAFAAFVRELSAAFKPKNLLLSAAVSPSKAVIDAGYDVPALSQYLDWIAVMTYDFHGHWDKQTGHVAPLYYYPGDVYDYFNANFSLNYWIEKGADPGKIVMGMPLYGQSFSLADAADRGLNAKSYGPGEAGEFTRAGGFLAYYEICERVKKRGWTVTRDPQGRIGPYAYLSNQWVSYDDIAEIRRKSRLVKALKLGGGMVWALDLDDFKNRCGCGKHPLLKTLNNELRGTPSDISVTNCT

Target ORF information:

RefSeq Version	XM_008199916.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum chitinase 10 (Cht10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008199916.2

ORF Insert Sequence:

ATGGAATTAC TGCACATAAC TCTATGTGCG TTCTTAATCC TCACACCGAC CGACCAAAGG 
GTCGTCACAA GGACAATCCC GAGCTTCCTT CGGGACGACG TCGAGCGATA TCCCTCAGGA 
CCCGAGCTTT CGGACAGGAG CGCCGCTTAC ACAAATCGGG AACTGTACGG GACGCGGGCT 
TTGCCGCTCC GTTCGGCGGT TGAACACATC CCGGATTTGA TTGCGGACTC GCGCCGGCTG 
CCACTCAGGG ACGCGGTGGA GAAACGCCCA CCGCCCGACG AACCAATCGA CGATTACGAA 
CCGAGCTTTG ATCTGTTCTA CTACCCGTCC CGACAAACTC TCTACACCCC AGTCCAGTAT 
TTCAAGGATT TGGTGGCCGA GGCGTACGTT TCCCCCTTTC GCATTGACAA CGAATCGCAG 
GAACTGCTAA AGTACCCCTC CTTTGCCCGG GGGCGGAGTT TGTATGACTC CAGAGGGAGG 
CACTCAGAGA TAGTTGAGAC GGTTTATGAC CCACGGGAGG ATGACCGTGT TGTTTGCTAC 
GTCCAAGCGG CTGCCAGGTA CCGGAAGGAG CCCTTGGCCT TCTCCCCCGA GGATTTGGAC 
CCCTTTGCTT GCACCCATGT CATTTATGCG TTCGCGACCA TTGACCCCCA CAATTTTAAC 
ATGATTTCGA ACGACGACGA GTCTGATATT ATCCAAGGGG GCTATATTTC CGTCACGGGA 
CTCAAAAGAG TTAACCCGAA ACTAAAAGTA CTGATCTCGG TTGGTGAGGG ACGGGATGGT 
TCCCACAGGT TCAGTACCAT GGTCTCCAGT GCCAACAGAC GAAGGGAGTT CATCCGGTCC 
GCCATCACTT TCATCAAGCA GTACAACTTT GACGGAATGG ACATCCACTG GGAGTACCCA 
GGGGCCGAGA AAATGGGCGG GCAACTGAGC GATAAGGAGT ACCTGAACCT CTTCCTCGAG 
GAGTTATCTG AAATTTTCAA ACCCAGAGGT TGGGTACTTA CAATTGCGGT ACCAGCCTCC 
CGGTTCCGGG TCGAAGATGG GTTTAACCCC CAACGCTTGG GGTCCCTTGT CGATTTTATC 
AACCTCCAAG CGTTTGATTT CCACCGGGAG CGGGAACCGG TGGCCGACCA CCACGCAAAT 
TTGTACTCAA GGCCGGGCGA TAGTGGCCTT GACCTGTTCC TAAGTGTGGA CTATGCTGTG 
AAGTTCTGGA CCAAGAAGGG GTTCCCCAAG TCCAAGATCG TGCTCGGGGT ACCATTTTTC 
GGGCGTTCGT TCACCCTCCA GTTCACAAAC GAGACGCAAG TTGGGGCCCC GATTAAAGGT 
CCAGGACGGG AAGGGTTCTA TACGCAAAAT CCAGGCTTCC TGGCATATTT CGAAATCTGC 
GATATGTTAC TGAATGAAGG GTGGCACAAA GGGATGGACG AGTCAGGGTC GCCTTACATG 
GTATATGGGG ACCAATGGGT GGGTTATGAC GATAGCGAAA GCGTCGAAAA GAAGGTTAAT 
TACGTGCAAG AGATGAACCT CGGGGGTGTG TTTATTTGGG CTACGGATCT GGACGATTTT 
AAGGGCGTTT GCGGAATGAA ATGGCCCTTG CTGAGTACCA TTAACCGGCA TTTGAGGGGA 
AACGAGTTGT TACCGATGCA ACAAAGCCAA AACCCTCCCA CTAAACCATA TGGTACTTGC 
CGCTCAGAAG GGATCTTCAG TGACCCCAAG AACTGTGCAG CTTATTACGT TTGTCGCTCA 
GGGTTGAGCT ACCACTTGTC GTGTGCCGAG AACATGATGT TTGACCCAAT GTCCGGGAAA 
TGTGAGTACA GCCTGGGGGA GAAGTGTAGG CCGGGTCAGA TCATCCAAGT CGCTAACTCT 
CTAAGACAAT ATGAGGACCT CCTGGAGTAT GAAGCTAAAG AGGAAGGACC AAAAGTGGTG 
TGCTACATGA CCAACTGGGC CTTCTACCGA AAAGCCGAAG GCAAATTCGT CCCGGAACAC 
ATCGACCAAA GGCTTTGCAC ACACGTGGTG TACGCCTTCG CGAGTCTCGA CCCCGAAAAA 
CTCCTCCTGA AGGAGTTCGA TCCTTGGGCC GACTTGGACA ACAATTTGTA CGAAAGAGTC 
ACCTCTTTGA AGGACACCAA GGCGATTTTA TCTCTTGGAG GCTGGACTGA CTCAGCTGGA 
GACAAGTACT CACGTCTTGT GGGCGATGGT TCCGCCAGAC GCCGGTTTGT TGTGGCAGTT 
GTGGGGTTTT TGCGACGACA CAATTTCAAA GGTTTGCATT TGGATTGGAA CTACCCCGTT 
TGTTGGCAAA GCAATTGCAA AAAAGGGGCG TCCTCCGATA AACCCAACTT CACCAAATTG 
ATTCAGGAGT TGCGGCGCGA GTTCGACAAA CAGAAACCTC CCCTGATCCT GGCTGCGGCC 
ATTTCGGGGT ATAAGGAGGT GATAGACGTG GCGTACGACC TACCAGCGCT TGGTACCGTC 
CTTGATTTCA TGTCAGTGAT GACTTATGAC TACCATGGGG CTTGGGAACG CCAAACGGGG 
CACGTGAGTC CGCTGTTTCA CCGGCCTGGG GATAAGTACC CCCAGTACAA CACTAACTTC 
ACCATGGAGT ATTTGGTGTC TCGTGGTGCT CCCCGTGGGA AACTGCTAGT CGGAGTACCA 
TTCTATGGTC AAAGTTTTAC CCTAACCAAG GGCAACAACC ACGACCAGGG AGTCCCAAGT 
GCCGGTCCCG GCGAAGCCGG TGAATACACC AAACAGCCAG GAATGTTGGC CTATTACGAA 
ATTTGTAACC GGATCCGGAA CCAACGCTGG ATCGTCAATC GGGACACTTC GGGTGCCACC 
GGCCCATTTG CCTACCATGG GGACCAATGG GTCGGTTTCG AGGACATCAA GTCGGTCCGA 
GACAAGGCCG AATACATCAA AACCAAAGGT TTTGGCGGCG CCGTGGCCTG GACTATCGAC 
TTGGACGACT TCTCCAACCG TTGTTGCGGT GGTTCCTTCC CTTTACTTCG AAGTTTGAAC 
CGTGGCTTGG GATTAATCCC CGATAATCCC TCACGAGAGG ACTGCACCAA ACCACCGGAA 
CCCGTAACTC CAGCTCCTCC ACAAGTCACA ACTGGGGTTG ATACGGGAGC TTCAAGTACC 
GAACACATGC ATCCAGACTG GACGACCAAA CCCAGCACCT GGTGGTCGAG TACTACGACA 
AGTCCGTGGT GGACCACTAC CACAACGAGG AGGACCACTA CGACTCGACC TACCACGACT 
AGTACCACGA CAAGGCCAAC TACGACCAAT TGGCCGACCC AAGGAACCAC AATCCCGCCC 
CCGGCTGTTG TAATGCCAGA AGTGGATAAA CCATCACAAC CATGCGAACC TGGGCAATAC 
GTACCAGACC CACACAATTG TAACGCTTAT TACAGGTGTG TCCTTGGGGA GTTGCGCAAG 
CAGTACTGCG CTGGTGGGCT TCATTGGAAC AAGGAACGCA AAATTTGTGA TTGGCCAAAA 
TCGGCCAAAT GCGAGGAGAA AAAACCTGGT CATAAACCTA GCACTTCGTC GTGGCAAAAA 
CCAACAAAAC CATCGTACCG CCCCCCAAGT ACCACCAACC ATTGGCAAAC CAAAACCACG 
ACTAGTACCA CGACCAGACC CACAACAACA GTATCACAAC TAATTGATGA TAAATGTGAT 
TCGGGCCAGT ACTACCCTCA CGAGTCATGC TCAAGCTTTT ATGTTTGTGT TAATGGGCAC 
TTGGTACCGC AGAATTGTGC CCCAGGGCTT CACTATAATC CGGAAGAACA CATGTGTGAC 
TGGAAGTACA AAGTCAAGTG TGTTGGGCGC AAACAATTGG CCCAGAAGTA CCAACTCCCC 
AAAATGGGGG GACCACAACC CTACTCCGCC TGCAGCGAAA ATGCCTTTGC CGCATACCCC 
GGGGATTGTA CCCGATATTT GCACTGTCTT TGGGGCAAAT ATGAAGTGTT TAACTGTGCC 
CCTGGCTTAC ACTGGGATAA TAATAAAAAT ATTTGTGATT GGCCTGAGAA AGCAACCTGT 
GATGGTACCA GCAACGTCAA TGTTGTTGAT ATCGTGACAA CAGCCAAACC CGCACAAAGT 
ACGACAAGTG TGTCAACTAC TACAAGTTGG AATCCGGGCA GTACCACCAA TTACCCGGAA 
TGGCAACCCA CGGAGTGGCA CCCTCCCATC CCCCCCACGT CCGAAAAACC GCCCCTTCCC 
GAAGAATTAA AACCACAATC CGGGTACTTT AAGATAGTTT GTTATTTCAC AAACTGGGCG 
TGGTACCGCA AAGGTTTAGG AAAGTACCTC CCGGAGGATA TCGACCCAGA CCTCTGCACC 
CACATCGTTT ACGGTTTCGC CGTCTTGGAC TTTGAAAATT TGATAGTGAA AGCGCACGAC 
TCGTGGGCCG ACTTTGATAA CCAATTCTAC AAACGCGTCA CTGGATACAA AGCCAAGGGA 
ATCAAAGTTT CCCTTGCCCT AGGCGGCTGG AACGACTCGC AAGGCGATAA GTACTCCCGC 
CTGGTCAATA ATCCCGCCGC ACGTGCCAGG TTTATCAAAC ACGTGTTGCA ATTTTTGGAA 
AAATGGAACT TTGACGGGCT TGATTTGGAC TGGGAGTACC CGAAATGCTG GCAAGTGGAT 
TGCAAGAAAG GCCCAGATTC GGACAAACAA GCCTTTGCTG CGTGGGTCAC CGAACTGAAG 
CAGGCCTTCA AACCGAAGGG ATATCTCCTC TCAGCTGCGG TTTCCCCAAG TAAGACTGTG 
ATAGATGCAG GGTATGATGT TCCAGTACTT GCGCAAAACC TGGACTGGGT GGCTGTGATG 
ACGTACGACT TCCATGGGCA GTGGGACAAG CAGACGGGTC ACGTGGCCCC GCTGTACTAC 
CATCCCGAGG ATGAGGTTGT CTTCTACAAC GCGAACTTCT CCATTAATTA CTGGATCTCG 
GAAGGAGTAC CGCGGAGGAA AATCGTGATG GGCATGCCCC TCTACGGCCA GTCCTTCCGC 
TTGGAGAAGC CCGAAAACCA CGGTTTGAAC GCCAAAGCCC CAGGTCCAGG CCAAGCCGGG 
GAATACACCC GAGCTGCCGG TTTCCTGGCT TATTACGAAA TCTGCCACAA TATCAAAACA 
CAAGGCTGGA CTGTGGTACA AGACCCCAAT CGACGCATGG GTCCCTACGC GTACAAAGGG 
GACCAATGGG TGTCGTTCGA CGATAGGGAG ATGATCAAGC GCAAATCGGA GTTTATCAGG 
AAGATGGATT TAGGTGGTGG GATGATCTGG GCCCTTGACT TGGACGATTT TAAGAACCGG 
TGTGGTGAAG GCCGACATCC CCTTCTGACC ACCATTAGGA ACGTCCTTGC GGATGCTGGA 
ACCGGGCAAC AGGAGACAAT TCCGCCCATT GATGAGGAAC CACCAAGTGT TGAAGCCGTG 
GAAGAGGAAC GTGAAGAAGC ACCTCCAAGT GTGCACTCTT CAACGGTGGT ACCACCACCG 
CAACATTCCA CAACGCAAAG TCTAGTCGAT CCAAAATCCG AGTTCAAGGT GGTTTGTTAT 
TTCACGAATT GGGCGTGGTA CCGGCAAGGT GTCGGGAAGT ACCTCCCTTC CGATATAGAC 
CCAGACTTGT GTACCCACAT CGTTTATGGA TTTGCGGTAC TCAACGGCGA TCAATTGATC 
ATCAAACCGC ACGACACTTG GGCCGATTTC GACAATAAGT TCTACGAAAA GGTCACAGCC 
TACAAATCGA AGGGAATCAA GGTACTTGTC GCAATCGGAG GCTGGAACGA CTCGGCAGGT 
GACAAGTACT CGCGTCTGGT GAACAACCCA TCGGCCCGCA GACGTTTCAT AGCCCACGTT 
GTCGATTTCA TCGAAACCAA CAACTTTGAC GGTTTGGACC TAGACTGGGA GTACCCCAAG 
TGTTGGCAAG TCGATTGTAA CAAAGGCCCG GCGTCTGACA AGTCAGCGTT TGCCGACTTT 
GTCAAAGAAT TGCACGAAGC CTTCAAACCC AAAGGGTGGC TCCTATCAGC TGCAGTTTCG 
CCAAGTCGGC GCGTGGTAGA TGCAGGGTAC GATGTACCAA CCTTGTCCAA GTACCTGGAC 
TGGATTGCCG TCATGTGCTA CGATTACCAT GGGCAATGGG ACAAGATTAC GGGGCACGTG 
GCGCCCATGT ACGCCCATCC CGAGGACGCG GACGCCACTT TCAATACTAA TTTCACTATT 
CACTACTGGA TTGAGAAAGG CGCTGATCGG AGGAAACTCG TGATGGGCAT GCCCATGTAT 
GGACAGTCGT TTTCATTGGC TGACAACAAC CAAAATGGGC TCAATGCAGC CACCTATGGG 
GGCGGAGAGG CCGGGGAGGA AACACGAGCT CGTGGTTTCC TAGCCTATTA TGAAATTTGT 
ACGAACGTTA TTAAGAAAGG GTGGAAGGTG GTACGTGACC GCCGGGGACG TATTGGTCCC 
TACGCCTACC TACGTGACCA ATGGGTCTCC TTCGACGATA TAGGCATGAT AAGACACAAG 
TCTGAGTTTA TCAAGGCCAT GGGTCTTGGT GGCGGTATGA TCTGGGCGTT GGATTTGGAC 
GATTTTAAAA ACCTTTGTGG GTGTGAGGAG TACCCACTAC TTCGCACCAT CAACCGGGTG 
TTACGAGGGT ACGCTAAACC TGACCCTAAG TGTATCCTAG GCCGTGATAA CGACAAAACT 
AAACCTAAAC CTACAACAAT GAAACCAACC ACAACCATTG GTTATGAACC ACAAGAACCG 
GTACTTCCGG CCGAAGTGGT ACCGTGTCAA GGCCGGCTTT TCGTAGCCGA TGATACCAAC 
TGTGCCCAGT ACTATTTGTG TAACCAGGGC CAGCTCCAAT TGCAAGTGTG CCCCAATGGT 
TTGTTCTGGA ATAAGGACCA TTGTGACTGG CCTGAGAACA CGGAGTGTCA CCCGGACGCG 
AGCAGTACCA TGGCACCATC AACCACACCG ATGGTACCGG ACAAGCCGGT CACAACCACC 
ACGTCCAGAC CCATTGGTAC TGACACCAGC GACGATTATA AGGTCGTTTG TTACTTTACC 
AACTGGGCGT GGTACCGCCA AGGCGATGGG AAGTACCTCC CGTCTGACAT AGACCCCTCA 
CTTTGTACTC ACATTAATTA CGGTTTTGCG GTCCTGGACT CGTCGAGTAT GACCTTAAAA 
CCGCACGATT CTTGGGCCGA TATCGACAAC GAGTTTTACA AAAAAGTCGT TTCGTACAAA 
TCGCGCGGTG TTAAGGTCTT GATAGCCTTA GGCGGTTGGA ACGACTCAGC CGGTAACAAA 
TACTCGCGCC TAGTCAACGA CCCACAAGCC CGTGCGGCCT TCATAGCCCA CGTTCTGGCC 
TTTATCGAAG AGTGGAACTT CGATGGCCTT GATTTAGACT GGGAGTACCC CAAATGTTGG 
CAAGTGGACT GCAACAAAGG CCCAGACTCG GACAAAGAAG CCTTCGCGGC CTTTGTCCGC 
GAACTGAGCG CGGCCTTCAA GCCCAAAAAC CTCCTACTAT CAGCTGCGGT TTCGCCCAGC 
AAGGCAGTGA TAGACGCTGG GTACGACGTG CCAGCCTTAT CCCAGTACTT GGACTGGATC 
GCCGTCATGA CCTACGATTT CCACGGCCAC TGGGACAAAC AAACCGGACA TGTGGCACCC 
TTGTATTACT ACCCTGGCGA TGTTTACGAT TATTTCAACG CCAACTTTTC GCTAAACTAC 
TGGATCGAGA AAGGGGCGGA CCCAGGAAAA ATCGTGATGG GCATGCCCCT CTACGGCCAA 
AGTTTCAGTC TGGCTGACGC GGCCGATAGG GGCCTTAACG CCAAGTCGTA CGGCCCTGGA 
GAAGCCGGGG AGTTTACCCG AGCTGGTGGT TTCCTAGCCT ACTATGAGAT TTGTGAACGG 
GTGAAGAAGA GGGGTTGGAC GGTGACGAGG GACCCTCAAG GTCGCATTGG TCCATATGCC 
TATTTGTCGA ACCAATGGGT GTCGTACGAT GATATCGCCG AAATCCGGCG CAAGTCGCGA 
TTGGTCAAGG CGTTGAAACT GGGCGGGGGC ATGGTCTGGG CTTTGGATTT GGACGATTTT 
AAGAACAGGT GTGGGTGTGG CAAACACCCG CTCTTGAAGA CTTTAAACAA TGAGTTGAGA 
GGGACGCCCT CTGATATCAG CGTGACTAAT TGTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny, expression and function.
Insect biochemistry and molecular biology40(3)214-27(2010 Mar)
Jasrapuria S,Arakane Y,Osman G,Kramer KJ,Beeman RW,Muthukrishnan S

Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects.
Insect biochemistry and molecular biology38(4)452-66(2008 Apr)
Zhu Q,Arakane Y,Banerjee D,Beeman RW,Kramer KJ,Muthukrishnan S

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Cht10 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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