Chs1 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chs1
Entrez Gene ID	641504
Full Name	chitin synthase 1
Synonyms	TcChs1
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008195966.2	XP_008194188.1	chitin synthase 1 isoform X1
NM_001039402.1	NP_001034491.1	chitin synthase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum.
Hayakawa Y, Kato D, Kamiya K, Minakuchi C, Miura K
Journal of invertebrate pathology14326-34(2017 02)

Characterization of two chitin synthase genes of the red flour beetle, Tribolium castaneum, and alternate exon usage in one of the genes during development.
Arakane Y, Hogenkamp DG, Zhu YC, Kramer KJ, Specht CA, Beeman RW, Kanost MR, Muthukrishnan S
Insect biochemistry and molecular biology34(3)291-304(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg15651	NM_001039402.1 Latest version!	Tribolium castaneum chitin synthase 1 (Chs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTg07253	XM_008195966.2 Latest version!	Tribolium castaneum chitin synthase 1 (Chs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chs1 ( NM_001039402.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001034491.1 Tribolium castaneum chitin synthase 1 (Chs1), mRNA.

Price & Availability↑

CloneID	OTg15651
Clone ID Related Accession (Same CDS sequence)	NM_001039402.1
Accession Version	NM_001039402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Tribolium castaneum(red flour beetle)
Product	chitin synthase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY291476.1. ##Evidence-Data-START## Transcript exon combination :: AY291476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1523770, SAMEA1523771 [ECO:0000348] ##Evidence-Data-END##

ATGACATCCG GGGGGCTGCG GAATGATACA TACAGCGATG ACAATTTCTC GGATGACGAG 
AGCTCCCCTC TCACCGAAAA CATTTATGGA GGAAGCACCA GGACTGTGCA AGAGACCAAA 
GGATGGGACG TGTTCAGAGA CCCCCCGATC AAGGAAGAGT CGGGTTCGAT GGCGAACCAA 
AAATGCCTCG AAATCACCGT CAAAATCCTC AAAATCGTAG CGTACCTCGT AACCTTCGTG 
ATAGTCCTCG GCTCGGGCGT CATCTCCAAA GGCACGCTCC TCTTCATGAC ATCCCAGCTC 
AAACCCGACA AAGTCACCGT ATTCTGTAAC CGGGACCTGG GCCGCGAGAA ACAATTCATC 
GTCAAGCTCC CATCGGCCGA GCGCGTGGCC TGGATGTGGT GCTTGATCTT CGCCTTCTGG 
GTGCCCCAGC TCGGCTCCCT GTTCCGCTCC TCCAGGATGT GCTTCTTCAA ATCGTCCAAA 
AAGCCCGCCT TCTCCCACTT CCTCATCGTC TTCTTCACCG AAACGCTCCA CACCGTCGGC 
ATCGCCCTCC TGATATTCTA CATTCTGCCG GACCTGGACG TGGTCAAAGG GGCCATGCTG 
ACCAACTGCA TTTGCTTCGT CCCGGGGGTT TTGGGCTTGC TGTCCCGCAG CAACAAGGAA 
TCGCAGAGGT TCCTCAAAGT GATAATCGAT TTGTTCGCAA TTGCGGCGCA AGCCACGGGC 
TTCGTCGTCT GGCCCATAAT TGAGCAAAGG GCCGACCTCT GGATCATCCC AATTGCCATC 
TTCCTGGTCT CGCTCGGCTG GTGGGAGAAC TACATTTCCA GGCACTCCCC CATCCCCTTT 
ATCAAAAAAC TGGGCAAAAT CAAGGAGAAC CTCGAGCAGA GTCGCTACTT CATCTACATG 
TTCGTCTCAG TCTGGAAATG TCTACTGTTC TTTGTCTCCG TCTTGTTCAT CCTCTTGGTC 
AAAGAAGGAG AAGTCGCGTT CTTTTTCACC CAGTTTTCCG AAGGCTTCAG CACCCACCAA 
ATCCAGGTCC TGGAGATCAA GCCCGTGCTA GGCGGGACGG CGCTTCCCGA CATTTCCGAG 
ATTATCCCAA CTGGGGACGA CACCACAATG GACTCGAACG ACATGACTGC GATTTACGTC 
CTCCTTATCA ACATTTTCGC CTCGTATTTC GCCTACATTT TCGGGAAATT CGCTTGCAAG 
ATCATGATCC AGGGCTTTTC GTACGCTTTC CCCGTAAATT TAACAATCCC AGTCTCGATT 
TCGCTGCTGA TCGCGGCTTG CGGGCTCCGC AACGGCGATC CCTGTTTCTT CCACGACACC 
ATCCCTCCGT ACCTCTTCTT CGAGAGTCCA CCGGTCGTGT TTTTGAACGA CTTCATCTCG 
CACCAACACG CCTGGATCTG GCTGCTGTGG TTGCTGTCCC AAACGTGGAT CACGTTGCAC 
ATTTGGACCC CGAAATGCGA GCGTTTGGCT CGGACTGAGA AACTTTTCGT CACCCCGATG 
TACGAGGGGC TGCTCATTGA TCAAAGTTTG GGGATGAATC GGAGGCGCGA CGATGAGGCT 
GATGTCAAGA CTGAGGATTT GGACGAGATC CAGAAGGAGA AAGGGGATGA GTATTACGAG 
ACCATCTCCA ACCACACGGA CGCTTCCTCG GCCAAAGCGG TCAAAAACTC CGACCACATC 
ACACGGATTT ATGCCTGCGC GACCATGTGG CACGAGAATA AGGAGGAAAT GATGGAGTTT 
TTGAAGTCGA TTTTGCGACT GGATGAAGAC CAGTCTGCCA GAAGGGTGGC TCAGAAGTAC 
CTCCGGGTGG TCGATCCGGA TTATTACGAA TTCGAAACTC ATATATTTTT CGACGACGCG 
TTCGAAATCT CCGACCACAA CGACGACGAA ACGCAAGTCA ACCGCTTCGT CAAGCTTTTG 
GTGGCGACCA TCGACGAAGC GGCGTCCGAC GTGCACCAGA CGCACATGCG CATCCGTCCC 
CCTAAGAAAA TCCCGACCCC GTACGGGGGT CGTCTGGTGT GGACGCTCCC CGGGAAAACG 
AAAATGATCG CCCATTTGAA GGACAAGATG AAGATAAGGC ACCGGAAGCG GTGGTCGCAA 
GTCATGTACA TGTACTACCT CCTGGGGCAC CGGCTCATGG AACTGCCAAT TTCCGTGGAC 
AGGAAAGCCG TGATCGCCGA AAACACGTAC CTGCTCACGC TTGACGGCGA CATCGATTTC 
CAGCCTTCGG CAGTATTGCT CTTGATTGAT TTGATGAAAA AGAACCGGAA CCTGGGGGCT 
GCCTGTGGAC GCATCCATCC GGTGGGCTCG GGACCTATGG TGTGGTACCA AATGTTTGAG 
TACGCCATTG GGCATTGGCT GCAAAAGGCC ACCGAGCATG TTATCGGCTG TGTGCTTTGT 
AGTCCCGGTT GTTTCTCGCT TTTTAGGGGA AAAGCGCTGA TGGATGATAA CGTTATGAAG 
AAATACACCA CTTCCTCGTC GGAAGCCAGG CATTACGTGC AATATGATCA GGGAGAAGAT 
CGTTGGTTGT GCACTCTGTT GCTCCAAAGA GGCTACCGAG TCGAATATTC GGCCGCGTCA 
GACGCCTACA CCCATGCACC TGAAGGTTTC AACGAGTTCT ACAACCAGCG GAGGAGATGG 
GTGCCTTCGA CCATCGCCAA TATCATGGAC TTGCTCATGG ATTCAAAACG AACGATCGAA 
ATTAACGACA ACATTTCCAT GCCTTACATT GGCTACCAAA TTCTTTTGAT GGGTGGGACC 
ATCCTGGGCC CCGGCACCAT CTTCCTCATG TTGGTGGGTG CCTTCGTGGC CGCGTTCCAA 
ATCGACAACT GGACGAGTTT CTACTACAAC ATCATCCCGA TCCTCTTTTT CATGCTGGTG 
TGTTTCACGT GCAAATCCAA CATTCAACTA ATCGTCGCCC AAATCTTATC AACCGGATAC 
GCTCTGATAA TGATGGCGGT AATAGTGGGT ACAGCGCTTC AGTTACGCGA GGACGGGGTC 
GGTTCCCCCT CTGCGATATT TTTAATAGCA ATGACTGGGT CATTTTTCAT CGCTGCTTGT 
CTCCACCCGC AAGAATTTTG GTGCATTGTC CCTGGAATTA TCTATCTGCT GTCGATTCCC 
TCCATGTATT TGCTACTGAT TTTGTACTCC ATTATCAATC TTAACGTCGT ATCTTGGGGT 
ACTCGTGAAG TCGCGGTGAA GAAAACGAAG AAAGAACTAG AGGAAGAGAA GAAACAGGCC 
GAGGAGGCCA AACGAAAAGC GAAGCAAAAG TCGCTGCTTG GGTTCCTCCA AAGTGGGGGC 
ACTAGTGACG ATGATGAGGG AAGTATCGAG ATTTCCTTAG CCGGGCTTTT CAAATGCATG 
TTGTGCACGC ATCAGAAGGC CGGCGATGAA AAAGCGTCCC TGATCAACAT TGCCGACTCG 
TTGGAAATGC TCAACAAGCG CCTGGACCAC ATTGAGAAGA CCATTGACCC CTCCGGTCAC 
ATTTCGAGGA GAAGGAGCAT GTCGGCGTCG TCAAGAGGTG ACCACCACCA CCTTGGAGCC 
GTGACTGAGG AAGGGGGCGA CGAATCAGCC AATGAGACTG ATTCGGAAAC GGTGTCCACA 
GTCCCACAAA ACAAGCGCGA TGACCTGGTC AATCCCTACT GGATTGAAGA CCCCGATGTG 
AGAAAGGGCG AAGTGGAGTT CTTGAGCAGC ACCGAAATCC TCTTCTGGAA GGATCTACTC 
GATAAGTACT TGTACCCCAT TGACGAGAAC AAGGAGGAGA AGGCCAGGAT TGCAAAGGAG 
TTGATCGAAC TCCGAAATAA GTCGGTTTTT GCGTTCTTCA TGTTCAATGC TCTCTTTGTC 
TTAGTCGTCT TCCTGTTGCA ACTTAATAAG GATCAGATCC ACGTTAAGTG GCCGCTAGGT 
GTACGAACTA ACATTACGTA TATTGAGGAA ACGTCCGAGG TCCACATTAG CAAAGAGTAC 
CTCCAATTGG AACCGATCGG TCTAGTCTTT GTCTTCTTCT TCGCCCTTAT TTTGGTGATC 
CAGTTCGTTG CAATGTTGTT CCATCGATTC GGAACAATCG CCCACATTTT GGCCTCGACA 
GAGCTCAACT TGTGTTGCAC CAAGAAAAAG GAGGAATTGT CCCCGAACGC ACTACTCGAC 
AAACAAGCCG TGGAAATCGT CAAACAGCTG CAAAAACTGC AAGGCATTGA CGACGGCGAC 
TACGAGAACG ACTCGGGCTC AGGCCCGGAC CGCATCGGCC GCCGCAAAAC CATCCACAAC 
TTGGAGCGCG CGGCTCAGAA GAAGCGCCAA ATCGGAACTC TGGATGTGGC TTTCAAGAAG 
CGATTCGCTA AACTTAACGC CAATGGGACG AACGCCGGGA CTCCGGTCCT GAGTCGAAGA 
CTGACCATGA GGAGAGAGAC GATGAAAGCG CTCGAAGTCA GGGTGAACTC GGTGATGGCC 
GAAAGGCGCA AATCACACAT GCAAACACTA GGTGCCAAAA ATGAGTACGG GAATAATAAT 
GTGGTGGCGA GGAATCACAG GAACAGTGTC GCTAGCAGCA TTCCGGCCAA GGATGTGTTC 
GAAAATGGAC ATGTGAACAA GGCGTTCGAA GAGGACAATT ATGACCATCG CAGGAACTCG 
CTACAAATGC AACAAAGAAA CAACGTTACC TGGAAAAGCA GCAATAGCAG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034491.1
CDS	194..4870
Translation	MTSGGLRNDTYSDDNFSDDESSPLTENIYGGSTRTVQETKGWDVFRDPPIKEESGSMANQKCLEITVKILKIVAYLVTFVIVLGSGVISKGTLLFMTSQLKPDKVTVFCNRDLGREKQFIVKLPSAERVAWMWCLIFAFWVPQLGSLFRSSRMCFFKSSKKPAFSHFLIVFFTETLHTVGIALLIFYILPDLDVVKGAMLTNCICFVPGVLGLLSRSNKESQRFLKVIIDLFAIAAQATGFVVWPIIEQRADLWIIPIAIFLVSLGWWENYISRHSPIPFIKKLGKIKENLEQSRYFIYMFVSVWKCLLFFVSVLFILLVKEGEVAFFFTQFSEGFSTHQIQVLEIKPVLGGTALPDISEIIPTGDDTTMDSNDMTAIYVLLINIFASYFAYIFGKFACKIMIQGFSYAFPVNLTIPVSISLLIAACGLRNGDPCFFHDTIPPYLFFESPPVVFLNDFISHQHAWIWLLWLLSQTWITLHIWTPKCERLARTEKLFVTPMYEGLLIDQSLGMNRRRDDEADVKTEDLDEIQKEKGDEYYETISNHTDASSAKAVKNSDHITRIYACATMWHENKEEMMEFLKSILRLDEDQSARRVAQKYLRVVDPDYYEFETHIFFDDAFEISDHNDDETQVNRFVKLLVATIDEAASDVHQTHMRIRPPKKIPTPYGGRLVWTLPGKTKMIAHLKDKMKIRHRKRWSQVMYMYYLLGHRLMELPISVDRKAVIAENTYLLTLDGDIDFQPSAVLLLIDLMKKNRNLGAACGRIHPVGSGPMVWYQMFEYAIGHWLQKATEHVIGCVLCSPGCFSLFRGKALMDDNVMKKYTTSSSEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHAPEGFNEFYNQRRRWVPSTIANIMDLLMDSKRTIEINDNISMPYIGYQILLMGGTILGPGTIFLMLVGAFVAAFQIDNWTSFYYNIIPILFFMLVCFTCKSNIQLIVAQILSTGYALIMMAVIVGTALQLREDGVGSPSAIFLIAMTGSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVAVKKTKKELEEEKKQAEEAKRKAKQKSLLGFLQSGGTSDDDEGSIEISLAGLFKCMLCTHQKAGDEKASLINIADSLEMLNKRLDHIEKTIDPSGHISRRRSMSASSRGDHHHLGAVTEEGGDESANETDSETVSTVPQNKRDDLVNPYWIEDPDVRKGEVEFLSSTEILFWKDLLDKYLYPIDENKEEKARIAKELIELRNKSVFAFFMFNALFVLVVFLLQLNKDQIHVKWPLGVRTNITYIEETSEVHISKEYLQLEPIGLVFVFFFALILVIQFVAMLFHRFGTIAHILASTELNLCCTKKKEELSPNALLDKQAVEIVKQLQKLQGIDDGDYENDSGSGPDRIGRRKTIHNLERAAQKKRQIGTLDVAFKKRFAKLNANGTNAGTPVLSRRLTMRRETMKALEVRVNSVMAERRKSHMQTLGAKNEYGNNNVVARNHRNSVASSIPAKDVFENGHVNKAFEEDNYDHRRNSLQMQQRNNVTWKSSNSRM

Target ORF information:

RefSeq Version	NM_001039402.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum chitin synthase 1 (Chs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039402.1

ORF Insert Sequence:

ATGACATCCG GGGGGCTGCG GAATGATACA TACAGCGATG ACAATTTCTC GGATGACGAG 
AGCTCCCCTC TCACCGAAAA CATTTATGGA GGAAGCACCA GGACTGTGCA AGAGACCAAA 
GGATGGGACG TGTTCAGAGA CCCCCCGATC AAGGAAGAGT CGGGTTCGAT GGCGAACCAA 
AAATGCCTCG AAATCACCGT CAAAATCCTC AAAATCGTAG CGTACCTCGT AACCTTCGTG 
ATAGTCCTCG GCTCGGGCGT CATCTCCAAA GGCACGCTCC TCTTCATGAC ATCCCAGCTC 
AAACCCGACA AAGTCACCGT ATTCTGTAAC CGGGACCTGG GCCGCGAGAA ACAATTCATC 
GTCAAGCTCC CATCGGCCGA GCGCGTGGCC TGGATGTGGT GCTTGATCTT CGCCTTCTGG 
GTGCCCCAGC TCGGCTCCCT GTTCCGCTCC TCCAGGATGT GCTTCTTCAA ATCGTCCAAA 
AAGCCCGCCT TCTCCCACTT CCTCATCGTC TTCTTCACCG AAACGCTCCA CACCGTCGGC 
ATCGCCCTCC TGATATTCTA CATTCTGCCG GACCTGGACG TGGTCAAAGG GGCCATGCTG 
ACCAACTGCA TTTGCTTCGT CCCGGGGGTT TTGGGCTTGC TGTCCCGCAG CAACAAGGAA 
TCGCAGAGGT TCCTCAAAGT GATAATCGAT TTGTTCGCAA TTGCGGCGCA AGCCACGGGC 
TTCGTCGTCT GGCCCATAAT TGAGCAAAGG GCCGACCTCT GGATCATCCC AATTGCCATC 
TTCCTGGTCT CGCTCGGCTG GTGGGAGAAC TACATTTCCA GGCACTCCCC CATCCCCTTT 
ATCAAAAAAC TGGGCAAAAT CAAGGAGAAC CTCGAGCAGA GTCGCTACTT CATCTACATG 
TTCGTCTCAG TCTGGAAATG TCTACTGTTC TTTGTCTCCG TCTTGTTCAT CCTCTTGGTC 
AAAGAAGGAG AAGTCGCGTT CTTTTTCACC CAGTTTTCCG AAGGCTTCAG CACCCACCAA 
ATCCAGGTCC TGGAGATCAA GCCCGTGCTA GGCGGGACGG CGCTTCCCGA CATTTCCGAG 
ATTATCCCAA CTGGGGACGA CACCACAATG GACTCGAACG ACATGACTGC GATTTACGTC 
CTCCTTATCA ACATTTTCGC CTCGTATTTC GCCTACATTT TCGGGAAATT CGCTTGCAAG 
ATCATGATCC AGGGCTTTTC GTACGCTTTC CCCGTAAATT TAACAATCCC AGTCTCGATT 
TCGCTGCTGA TCGCGGCTTG CGGGCTCCGC AACGGCGATC CCTGTTTCTT CCACGACACC 
ATCCCTCCGT ACCTCTTCTT CGAGAGTCCA CCGGTCGTGT TTTTGAACGA CTTCATCTCG 
CACCAACACG CCTGGATCTG GCTGCTGTGG TTGCTGTCCC AAACGTGGAT CACGTTGCAC 
ATTTGGACCC CGAAATGCGA GCGTTTGGCT CGGACTGAGA AACTTTTCGT CACCCCGATG 
TACGAGGGGC TGCTCATTGA TCAAAGTTTG GGGATGAATC GGAGGCGCGA CGATGAGGCT 
GATGTCAAGA CTGAGGATTT GGACGAGATC CAGAAGGAGA AAGGGGATGA GTATTACGAG 
ACCATCTCCA ACCACACGGA CGCTTCCTCG GCCAAAGCGG TCAAAAACTC CGACCACATC 
ACACGGATTT ATGCCTGCGC GACCATGTGG CACGAGAATA AGGAGGAAAT GATGGAGTTT 
TTGAAGTCGA TTTTGCGACT GGATGAAGAC CAGTCTGCCA GAAGGGTGGC TCAGAAGTAC 
CTCCGGGTGG TCGATCCGGA TTATTACGAA TTCGAAACTC ATATATTTTT CGACGACGCG 
TTCGAAATCT CCGACCACAA CGACGACGAA ACGCAAGTCA ACCGCTTCGT CAAGCTTTTG 
GTGGCGACCA TCGACGAAGC GGCGTCCGAC GTGCACCAGA CGCACATGCG CATCCGTCCC 
CCTAAGAAAA TCCCGACCCC GTACGGGGGT CGTCTGGTGT GGACGCTCCC CGGGAAAACG 
AAAATGATCG CCCATTTGAA GGACAAGATG AAGATAAGGC ACCGGAAGCG GTGGTCGCAA 
GTCATGTACA TGTACTACCT CCTGGGGCAC CGGCTCATGG AACTGCCAAT TTCCGTGGAC 
AGGAAAGCCG TGATCGCCGA AAACACGTAC CTGCTCACGC TTGACGGCGA CATCGATTTC 
CAGCCTTCGG CAGTATTGCT CTTGATTGAT TTGATGAAAA AGAACCGGAA CCTGGGGGCT 
GCCTGTGGAC GCATCCATCC GGTGGGCTCG GGACCTATGG TGTGGTACCA AATGTTTGAG 
TACGCCATTG GGCATTGGCT GCAAAAGGCC ACCGAGCATG TTATCGGCTG TGTGCTTTGT 
AGTCCCGGTT GTTTCTCGCT TTTTAGGGGA AAAGCGCTGA TGGATGATAA CGTTATGAAG 
AAATACACCA CTTCCTCGTC GGAAGCCAGG CATTACGTGC AATATGATCA GGGAGAAGAT 
CGTTGGTTGT GCACTCTGTT GCTCCAAAGA GGCTACCGAG TCGAATATTC GGCCGCGTCA 
GACGCCTACA CCCATGCACC TGAAGGTTTC AACGAGTTCT ACAACCAGCG GAGGAGATGG 
GTGCCTTCGA CCATCGCCAA TATCATGGAC TTGCTCATGG ATTCAAAACG AACGATCGAA 
ATTAACGACA ACATTTCCAT GCCTTACATT GGCTACCAAA TTCTTTTGAT GGGTGGGACC 
ATCCTGGGCC CCGGCACCAT CTTCCTCATG TTGGTGGGTG CCTTCGTGGC CGCGTTCCAA 
ATCGACAACT GGACGAGTTT CTACTACAAC ATCATCCCGA TCCTCTTTTT CATGCTGGTG 
TGTTTCACGT GCAAATCCAA CATTCAACTA ATCGTCGCCC AAATCTTATC AACCGGATAC 
GCTCTGATAA TGATGGCGGT AATAGTGGGT ACAGCGCTTC AGTTACGCGA GGACGGGGTC 
GGTTCCCCCT CTGCGATATT TTTAATAGCA ATGACTGGGT CATTTTTCAT CGCTGCTTGT 
CTCCACCCGC AAGAATTTTG GTGCATTGTC CCTGGAATTA TCTATCTGCT GTCGATTCCC 
TCCATGTATT TGCTACTGAT TTTGTACTCC ATTATCAATC TTAACGTCGT ATCTTGGGGT 
ACTCGTGAAG TCGCGGTGAA GAAAACGAAG AAAGAACTAG AGGAAGAGAA GAAACAGGCC 
GAGGAGGCCA AACGAAAAGC GAAGCAAAAG TCGCTGCTTG GGTTCCTCCA AAGTGGGGGC 
ACTAGTGACG ATGATGAGGG AAGTATCGAG ATTTCCTTAG CCGGGCTTTT CAAATGCATG 
TTGTGCACGC ATCAGAAGGC CGGCGATGAA AAAGCGTCCC TGATCAACAT TGCCGACTCG 
TTGGAAATGC TCAACAAGCG CCTGGACCAC ATTGAGAAGA CCATTGACCC CTCCGGTCAC 
ATTTCGAGGA GAAGGAGCAT GTCGGCGTCG TCAAGAGGTG ACCACCACCA CCTTGGAGCC 
GTGACTGAGG AAGGGGGCGA CGAATCAGCC AATGAGACTG ATTCGGAAAC GGTGTCCACA 
GTCCCACAAA ACAAGCGCGA TGACCTGGTC AATCCCTACT GGATTGAAGA CCCCGATGTG 
AGAAAGGGCG AAGTGGAGTT CTTGAGCAGC ACCGAAATCC TCTTCTGGAA GGATCTACTC 
GATAAGTACT TGTACCCCAT TGACGAGAAC AAGGAGGAGA AGGCCAGGAT TGCAAAGGAG 
TTGATCGAAC TCCGAAATAA GTCGGTTTTT GCGTTCTTCA TGTTCAATGC TCTCTTTGTC 
TTAGTCGTCT TCCTGTTGCA ACTTAATAAG GATCAGATCC ACGTTAAGTG GCCGCTAGGT 
GTACGAACTA ACATTACGTA TATTGAGGAA ACGTCCGAGG TCCACATTAG CAAAGAGTAC 
CTCCAATTGG AACCGATCGG TCTAGTCTTT GTCTTCTTCT TCGCCCTTAT TTTGGTGATC 
CAGTTCGTTG CAATGTTGTT CCATCGATTC GGAACAATCG CCCACATTTT GGCCTCGACA 
GAGCTCAACT TGTGTTGCAC CAAGAAAAAG GAGGAATTGT CCCCGAACGC ACTACTCGAC 
AAACAAGCCG TGGAAATCGT CAAACAGCTG CAAAAACTGC AAGGCATTGA CGACGGCGAC 
TACGAGAACG ACTCGGGCTC AGGCCCGGAC CGCATCGGCC GCCGCAAAAC CATCCACAAC 
TTGGAGCGCG CGGCTCAGAA GAAGCGCCAA ATCGGAACTC TGGATGTGGC TTTCAAGAAG 
CGATTCGCTA AACTTAACGC CAATGGGACG AACGCCGGGA CTCCGGTCCT GAGTCGAAGA 
CTGACCATGA GGAGAGAGAC GATGAAAGCG CTCGAAGTCA GGGTGAACTC GGTGATGGCC 
GAAAGGCGCA AATCACACAT GCAAACACTA GGTGCCAAAA ATGAGTACGG GAATAATAAT 
GTGGTGGCGA GGAATCACAG GAACAGTGTC GCTAGCAGCA TTCCGGCCAA GGATGTGTTC 
GAAAATGGAC ATGTGAACAA GGCGTTCGAA GAGGACAATT ATGACCATCG CAGGAACTCG 
CTACAAATGC AACAAAGAAA CAACGTTACC TGGAAAAGCA GCAATAGCAG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chs1 ( XM_008195966.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008194188.1 Tribolium castaneum chitin synthase 1 (Chs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTg07253
Clone ID Related Accession (Same CDS sequence)	XM_008195966.2
Accession Version	XM_008195966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	chitin synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450166.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008195966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCG GGGGGCTGCG GAATGATACA TACAGCGATG ACAATTTCTC GGATGACGAG 
AGCTCCCCTC TCACCGAAAA CATTTATGGA GGAAGCACCA GGACTGTGCA AGAGACCAAA 
GGATGGGACG TGTTCAGAGA CCCCCCGATC AAGGAAGAGT CGGGTTCGAT GGCGAACCAA 
AAATGCCTCG AAATCACCGT CAAAATCCTC AAAATCGTAG CGTACCTCGT AACCTTCGTG 
ATAGTCCTCG GCTCGGGCGT CATCTCCAAA GGCACGCTCC TCTTCATGAC ATCCCAGCTC 
AAACCCGACA AAGTCACCGT ATTCTGTAAC CGGGACCTGG GCCGCGAGAA ACAATTCATC 
GTCAAGCTCC CATCGGCCGA GCGCGTGGCC TGGATGTGGT GCTTGATCTT CGCCTTCTGG 
GTGCCCCAGC TCGGCTCCCT GTTCCGCTCC TCCAGGATGT GCTTCTTCAA ATCGTCCAAA 
AAGCCCGCCT TCTCCCACTT CCTCATCGTC TTCTTCACCG AAACGCTCCA CACCGTCGGC 
ATCGCCCTCC TGATATTCTA CATTCTGCCG GACCTGGACG TGGTCAAAGG GGCCATGCTG 
ACCAACTGCA TTTGCTTCGT CCCGGGGGTT TTGGGCTTGC TGTCCCGCAG CAACAAGGAA 
TCGCAGAGGT TCCTCAAAGT GATAATCGAT TTGTTCGCAA TTGCGGCGCA AGCCACGGGC 
TTCGTCGTCT GGCCCATAAT TGAGCAAAGG GCCGACCTCT GGATCATCCC AATTGCCATC 
TTCCTGGTCT CGCTCGGCTG GTGGGAGAAC TACATTTCCA GGCACTCCCC CATCCCCTTT 
ATCAAAAAAC TGGGCAAAAT CAAGGAGAAC CTCGAGCAGA GTCGCTACTT CATCTACATG 
TTCGTCTCAG TCTGGAAATG TCTACTGTTC TTTGTCTCCG TCTTGTTCAT CCTCTTGGTC 
AAAGAAGGAG AAGTCGCGTT CTTTTTCACC CAGTTTTCCG AAGGCTTCAG CACCCACCAA 
ATCCAGGTCC TGGAGATCAA GCCCGTGCTA GGCGGGACGG CGCTTCCCGA CATTTCCGAG 
ATTATCCCAA CTGGGGACGA CACCACAATG GACTCGAACG ACATGACTGC GATTTACGTC 
CTCCTTATCA ACATTTTCGC CTCGTATTTC GCCTACATTT TCGGGAAATT CGCTTGCAAG 
ATCATGATCC AGGGCTTTTC GTACGCTTTC CCCGTAAATT TAACAATCCC AGTCTCGATT 
TCGCTGCTGA TCGCGGCTTG CGGGCTCCGC AACGGCGATC CCTGTTTCTT CCACGACACC 
ATCCCTCCGT ACCTCTTCTT CGAGAGTCCA CCGGTCGTGT TTTTGAACGA CTTCATCTCG 
CACCAACACG CCTGGATCTG GCTGCTGTGG TTGCTGTCCC AAACGTGGAT CACGTTGCAC 
ATTTGGACCC CGAAATGCGA GCGTTTGGCT CGGACTGAGA AACTTTTCGT CACCCCGATG 
TACGAGGGGC TGCTCATTGA TCAAAGTTTG GGGATGAATC GGAGGCGCGA CGATGAGGCT 
GATGTCAAGA CTGAGGATTT GGACGAGATC CAGAAGGAGA AAGGGGATGA GTATTACGAG 
ACCATCTCCA ACCACACGGA CGCTTCCTCG GCCAAAGCGG TCAAAAACTC CGACCACATC 
ACACGGATTT ATGCCTGCGC GACCATGTGG CACGAGAATA AGGAGGAAAT GATGGAGTTT 
TTGAAGTCGA TTTTGCGACT GGATGAAGAC CAGTCTGCCA GAAGGGTGGC TCAGAAGTAC 
CTCCGGGTGG TCGATCCGGA TTATTACGAA TTCGAAACTC ATATATTTTT CGACGACGCG 
TTCGAAATCT CCGACCACAA CGACGACGAA ACGCAAGTCA ACCGCTTCGT CAAGCTTTTG 
GTGGCGACCA TCGACGAAGC GGCGTCCGAC GTGCACCAGA CGCACATGCG CATCCGTCCC 
CCTAAGAAAA TCCCGACCCC GTACGGGGGT CGTCTGGTGT GGACGCTCCC CGGGAAAACG 
AAAATGATCG CCCATTTGAA GGACAAGATG AAGATAAGGC ACCGGAAGCG GTGGTCGCAA 
GTCATGTACA TGTACTACCT CCTGGGGCAC CGGCTCATGG AACTGCCAAT TTCCGTGGAC 
AGGAAAGCCG TGATCGCCGA AAACACGTAC CTGCTCACGC TTGACGGCGA CATCGATTTC 
CAGCCTTCGG CAGTATTGCT TTTGATTGAT TTGATGAAAA AGAACCGGAA CCTGGGGGCT 
GCCTGTGGAC GCATCCATCC GGTGGGCTCG GGACCTATGG TGTGGTACCA AATGTTTGAG 
TACGCCATTG GGCATTGGCT GCAAAAGGCC ACCGAGCATG TTATCGGCTG TGTGCTTTGT 
AGTCCCGGTT GTTTCTCGCT TTTTAGGGGA AAAGCGCTGA TGGATGATAA CGTTATGAAG 
AAATACACCA CTTCCTCGTC GGAAGCCAGG CATTACGTGC AATATGATCA GGGAGAAGAT 
CGTTGGTTGT GCACTCTGTT GCTCCAAAGA GGCTACCGAG TCGAATATTC GGCCGCGTCA 
GACGCCTACA CCCATGCACC TGAAGGTTTC AACGAGTTCT ACAACCAGCG GAGGAGATGG 
GTGCCTTCGA CCATCGCCAA TATCATGGAC TTGCTCATGG ATTCAAAACG AACGATCGAA 
ATTAACGACA ACATTTCCAT GCCTTACATT GGCTACCAAA TTCTTTTGAT GGGTGGGACC 
ATCCTGGGCC CCGGCACCAT CTTCCTCATG TTGGTGGGTG CCTTCGTGGC CGCGTTCCAA 
ATCGACAACT GGACGAGTTT CTACTACAAC ATCATCCCGA TCCTCTTTTT CATGCTGGTG 
TGTTTCACGT GCAAATCCAA CATTCAACTA ATCGTCGCCC AAATCTTATC AACCGGATAC 
GCTCTGATAA TGATGGCGGT AATAGTGGGT ACAGCGCTTC AGTTACGCGA GGACGGGGTC 
GGTTCCCCCT CTGCGATATT TTTAATAGCA ATGACTGGGT CATTTTTCAT CGCTGCTTGT 
CTCCACCCGC AAGAATTTTG GTGCATTGTC CCTGGAATTA TCTATCTGCT GTCGATTCCC 
TCCATGTATT TGCTACTGAT TTTGTACTCC ATTATCAATC TTAACGTCGT ATCTTGGGGT 
ACTCGTGAAG TCGCGGTGAA GAAAACGAAG AAAGAACTAG AGGAAGAGAA GAAACAGGCC 
GAGGAGGCCA AACGAAAAGC GAAGCAAAAG TCGCTGCTTG GGTTCCTCCA AAGTGGGGGC 
ACTAGTGACG ATGATGAGGG AAGTATCGAG ATTTCCTTAG CCGGGCTTTT CAAATGCATG 
TTGTGCACGC ATCAGAAGGC CGGCGATGAA AAAGCGTCCC TGATCAACAT TGCCGACTCG 
TTGGAAATGC TCAACAAGCG CCTGGACCAC ATTGAGAAGA CCATTGACCC CTCCGGTCAC 
ATTTCGAGGA GAAGGAGCAT GTCGGCGTCG TCAAGAGGTG ACCACCACCA CCTTGGAGCC 
GTGACTGAGG AAGGGGGCGA CGAATCAGCC AATGAGACTG ATTCGGAAAC GGTGTCCACA 
GTCCCACAAA ACAAGCGCGA TGACCTGGTC AATCCCTACT GGATTGAAGA CCCCGATGTG 
AGAAAGGGCG AAGTGGAGTT CTTGAGCAGC ACCGAAATCC TCTTCTGGAA GGATCTACTC 
GATAAGTACT TGTACCCCAT TGACGAGAAC AAGGAGGAGA AGGCCCGTAT AGCCGCCGAC 
TTGAAGGAAT TGCGTGATCA ATCCGTGTTT GCTTTCTTTA TGATGAACGC TTTGTTCGTG 
CTGATAGTGT TCCTGCTGAC GTTGAAAAAG GACTACTTGC ACATAAAGTG GCCTTTCGGT 
GTAAAAACCA ACATAACCTA TGACGAGAGC ACCCAAGAGG TCCACATTAG CAAAGAGTAC 
CTCCAATTGG AACCGATCGG TCTAGTCTTT GTCTTCTTCT TCGCCCTTAT TTTGGTGATC 
CAGTTCGTTG CAATGTTGTT CCATCGATTC GGAACAATCG CCCACATTTT GGCCTCGACA 
GAGCTCAACT TGTGTTGCAC CAAGAAAAAG GAGGAATTGT CCCCGAACGC ACTACTCGAC 
AAACAAGCCG TGGAAATCGT CAAACAGCTG CAAAAACTGC AAGGCATTGA CGACGGCGAC 
TACGAGAACG ACTCGGGCTC AGGCCCGGAC CGCATCGGCC GCCGCAAAAC CATCCACAAC 
TTGGAGCGCG CGGCTCAGAA GAAGCGCCAA ATCGGAACTC TGGATGTGGC TTTCAAGAAG 
CGATTCGCTA AACTTAACGC CAATGGGACG AACGCCGGGA CTCCGGTCCT GAGTCGAAGA 
CTGACCATGA GGAGAGAGAC GATGAAAGCG CTCGAAGTCA GGGTGAACTC GGTGATGGCC 
GAAAGGCGCA AATCACACAT GCAAACACTA GGTGCCAAAA ATGAGTACGG GAATAATAAT 
GTGGTGGCGA GGAATCACAG GAACAGTGTC GCTAGCAGCA TTCCGGCCAA GGATGTGTTC 
GAAAATGGAC ATGTGAACAA GGCGTTCGAA GAGGACAATT ATGACCATCG CAGGAACTCG 
CTACAAATGC AACAAAGAAA CAACGTTACC TGGAAAAGCA GCAATAGCAG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008194188.1
CDS	206..4882
Translation	MTSGGLRNDTYSDDNFSDDESSPLTENIYGGSTRTVQETKGWDVFRDPPIKEESGSMANQKCLEITVKILKIVAYLVTFVIVLGSGVISKGTLLFMTSQLKPDKVTVFCNRDLGREKQFIVKLPSAERVAWMWCLIFAFWVPQLGSLFRSSRMCFFKSSKKPAFSHFLIVFFTETLHTVGIALLIFYILPDLDVVKGAMLTNCICFVPGVLGLLSRSNKESQRFLKVIIDLFAIAAQATGFVVWPIIEQRADLWIIPIAIFLVSLGWWENYISRHSPIPFIKKLGKIKENLEQSRYFIYMFVSVWKCLLFFVSVLFILLVKEGEVAFFFTQFSEGFSTHQIQVLEIKPVLGGTALPDISEIIPTGDDTTMDSNDMTAIYVLLINIFASYFAYIFGKFACKIMIQGFSYAFPVNLTIPVSISLLIAACGLRNGDPCFFHDTIPPYLFFESPPVVFLNDFISHQHAWIWLLWLLSQTWITLHIWTPKCERLARTEKLFVTPMYEGLLIDQSLGMNRRRDDEADVKTEDLDEIQKEKGDEYYETISNHTDASSAKAVKNSDHITRIYACATMWHENKEEMMEFLKSILRLDEDQSARRVAQKYLRVVDPDYYEFETHIFFDDAFEISDHNDDETQVNRFVKLLVATIDEAASDVHQTHMRIRPPKKIPTPYGGRLVWTLPGKTKMIAHLKDKMKIRHRKRWSQVMYMYYLLGHRLMELPISVDRKAVIAENTYLLTLDGDIDFQPSAVLLLIDLMKKNRNLGAACGRIHPVGSGPMVWYQMFEYAIGHWLQKATEHVIGCVLCSPGCFSLFRGKALMDDNVMKKYTTSSSEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHAPEGFNEFYNQRRRWVPSTIANIMDLLMDSKRTIEINDNISMPYIGYQILLMGGTILGPGTIFLMLVGAFVAAFQIDNWTSFYYNIIPILFFMLVCFTCKSNIQLIVAQILSTGYALIMMAVIVGTALQLREDGVGSPSAIFLIAMTGSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVAVKKTKKELEEEKKQAEEAKRKAKQKSLLGFLQSGGTSDDDEGSIEISLAGLFKCMLCTHQKAGDEKASLINIADSLEMLNKRLDHIEKTIDPSGHISRRRSMSASSRGDHHHLGAVTEEGGDESANETDSETVSTVPQNKRDDLVNPYWIEDPDVRKGEVEFLSSTEILFWKDLLDKYLYPIDENKEEKARIAADLKELRDQSVFAFFMMNALFVLIVFLLTLKKDYLHIKWPFGVKTNITYDESTQEVHISKEYLQLEPIGLVFVFFFALILVIQFVAMLFHRFGTIAHILASTELNLCCTKKKEELSPNALLDKQAVEIVKQLQKLQGIDDGDYENDSGSGPDRIGRRKTIHNLERAAQKKRQIGTLDVAFKKRFAKLNANGTNAGTPVLSRRLTMRRETMKALEVRVNSVMAERRKSHMQTLGAKNEYGNNNVVARNHRNSVASSIPAKDVFENGHVNKAFEEDNYDHRRNSLQMQQRNNVTWKSSNSRM

Target ORF information:

RefSeq Version	XM_008195966.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum chitin synthase 1 (Chs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008195966.2

ORF Insert Sequence:

ATGACATCCG GGGGGCTGCG GAATGATACA TACAGCGATG ACAATTTCTC GGATGACGAG 
AGCTCCCCTC TCACCGAAAA CATTTATGGA GGAAGCACCA GGACTGTGCA AGAGACCAAA 
GGATGGGACG TGTTCAGAGA CCCCCCGATC AAGGAAGAGT CGGGTTCGAT GGCGAACCAA 
AAATGCCTCG AAATCACCGT CAAAATCCTC AAAATCGTAG CGTACCTCGT AACCTTCGTG 
ATAGTCCTCG GCTCGGGCGT CATCTCCAAA GGCACGCTCC TCTTCATGAC ATCCCAGCTC 
AAACCCGACA AAGTCACCGT ATTCTGTAAC CGGGACCTGG GCCGCGAGAA ACAATTCATC 
GTCAAGCTCC CATCGGCCGA GCGCGTGGCC TGGATGTGGT GCTTGATCTT CGCCTTCTGG 
GTGCCCCAGC TCGGCTCCCT GTTCCGCTCC TCCAGGATGT GCTTCTTCAA ATCGTCCAAA 
AAGCCCGCCT TCTCCCACTT CCTCATCGTC TTCTTCACCG AAACGCTCCA CACCGTCGGC 
ATCGCCCTCC TGATATTCTA CATTCTGCCG GACCTGGACG TGGTCAAAGG GGCCATGCTG 
ACCAACTGCA TTTGCTTCGT CCCGGGGGTT TTGGGCTTGC TGTCCCGCAG CAACAAGGAA 
TCGCAGAGGT TCCTCAAAGT GATAATCGAT TTGTTCGCAA TTGCGGCGCA AGCCACGGGC 
TTCGTCGTCT GGCCCATAAT TGAGCAAAGG GCCGACCTCT GGATCATCCC AATTGCCATC 
TTCCTGGTCT CGCTCGGCTG GTGGGAGAAC TACATTTCCA GGCACTCCCC CATCCCCTTT 
ATCAAAAAAC TGGGCAAAAT CAAGGAGAAC CTCGAGCAGA GTCGCTACTT CATCTACATG 
TTCGTCTCAG TCTGGAAATG TCTACTGTTC TTTGTCTCCG TCTTGTTCAT CCTCTTGGTC 
AAAGAAGGAG AAGTCGCGTT CTTTTTCACC CAGTTTTCCG AAGGCTTCAG CACCCACCAA 
ATCCAGGTCC TGGAGATCAA GCCCGTGCTA GGCGGGACGG CGCTTCCCGA CATTTCCGAG 
ATTATCCCAA CTGGGGACGA CACCACAATG GACTCGAACG ACATGACTGC GATTTACGTC 
CTCCTTATCA ACATTTTCGC CTCGTATTTC GCCTACATTT TCGGGAAATT CGCTTGCAAG 
ATCATGATCC AGGGCTTTTC GTACGCTTTC CCCGTAAATT TAACAATCCC AGTCTCGATT 
TCGCTGCTGA TCGCGGCTTG CGGGCTCCGC AACGGCGATC CCTGTTTCTT CCACGACACC 
ATCCCTCCGT ACCTCTTCTT CGAGAGTCCA CCGGTCGTGT TTTTGAACGA CTTCATCTCG 
CACCAACACG CCTGGATCTG GCTGCTGTGG TTGCTGTCCC AAACGTGGAT CACGTTGCAC 
ATTTGGACCC CGAAATGCGA GCGTTTGGCT CGGACTGAGA AACTTTTCGT CACCCCGATG 
TACGAGGGGC TGCTCATTGA TCAAAGTTTG GGGATGAATC GGAGGCGCGA CGATGAGGCT 
GATGTCAAGA CTGAGGATTT GGACGAGATC CAGAAGGAGA AAGGGGATGA GTATTACGAG 
ACCATCTCCA ACCACACGGA CGCTTCCTCG GCCAAAGCGG TCAAAAACTC CGACCACATC 
ACACGGATTT ATGCCTGCGC GACCATGTGG CACGAGAATA AGGAGGAAAT GATGGAGTTT 
TTGAAGTCGA TTTTGCGACT GGATGAAGAC CAGTCTGCCA GAAGGGTGGC TCAGAAGTAC 
CTCCGGGTGG TCGATCCGGA TTATTACGAA TTCGAAACTC ATATATTTTT CGACGACGCG 
TTCGAAATCT CCGACCACAA CGACGACGAA ACGCAAGTCA ACCGCTTCGT CAAGCTTTTG 
GTGGCGACCA TCGACGAAGC GGCGTCCGAC GTGCACCAGA CGCACATGCG CATCCGTCCC 
CCTAAGAAAA TCCCGACCCC GTACGGGGGT CGTCTGGTGT GGACGCTCCC CGGGAAAACG 
AAAATGATCG CCCATTTGAA GGACAAGATG AAGATAAGGC ACCGGAAGCG GTGGTCGCAA 
GTCATGTACA TGTACTACCT CCTGGGGCAC CGGCTCATGG AACTGCCAAT TTCCGTGGAC 
AGGAAAGCCG TGATCGCCGA AAACACGTAC CTGCTCACGC TTGACGGCGA CATCGATTTC 
CAGCCTTCGG CAGTATTGCT TTTGATTGAT TTGATGAAAA AGAACCGGAA CCTGGGGGCT 
GCCTGTGGAC GCATCCATCC GGTGGGCTCG GGACCTATGG TGTGGTACCA AATGTTTGAG 
TACGCCATTG GGCATTGGCT GCAAAAGGCC ACCGAGCATG TTATCGGCTG TGTGCTTTGT 
AGTCCCGGTT GTTTCTCGCT TTTTAGGGGA AAAGCGCTGA TGGATGATAA CGTTATGAAG 
AAATACACCA CTTCCTCGTC GGAAGCCAGG CATTACGTGC AATATGATCA GGGAGAAGAT 
CGTTGGTTGT GCACTCTGTT GCTCCAAAGA GGCTACCGAG TCGAATATTC GGCCGCGTCA 
GACGCCTACA CCCATGCACC TGAAGGTTTC AACGAGTTCT ACAACCAGCG GAGGAGATGG 
GTGCCTTCGA CCATCGCCAA TATCATGGAC TTGCTCATGG ATTCAAAACG AACGATCGAA 
ATTAACGACA ACATTTCCAT GCCTTACATT GGCTACCAAA TTCTTTTGAT GGGTGGGACC 
ATCCTGGGCC CCGGCACCAT CTTCCTCATG TTGGTGGGTG CCTTCGTGGC CGCGTTCCAA 
ATCGACAACT GGACGAGTTT CTACTACAAC ATCATCCCGA TCCTCTTTTT CATGCTGGTG 
TGTTTCACGT GCAAATCCAA CATTCAACTA ATCGTCGCCC AAATCTTATC AACCGGATAC 
GCTCTGATAA TGATGGCGGT AATAGTGGGT ACAGCGCTTC AGTTACGCGA GGACGGGGTC 
GGTTCCCCCT CTGCGATATT TTTAATAGCA ATGACTGGGT CATTTTTCAT CGCTGCTTGT 
CTCCACCCGC AAGAATTTTG GTGCATTGTC CCTGGAATTA TCTATCTGCT GTCGATTCCC 
TCCATGTATT TGCTACTGAT TTTGTACTCC ATTATCAATC TTAACGTCGT ATCTTGGGGT 
ACTCGTGAAG TCGCGGTGAA GAAAACGAAG AAAGAACTAG AGGAAGAGAA GAAACAGGCC 
GAGGAGGCCA AACGAAAAGC GAAGCAAAAG TCGCTGCTTG GGTTCCTCCA AAGTGGGGGC 
ACTAGTGACG ATGATGAGGG AAGTATCGAG ATTTCCTTAG CCGGGCTTTT CAAATGCATG 
TTGTGCACGC ATCAGAAGGC CGGCGATGAA AAAGCGTCCC TGATCAACAT TGCCGACTCG 
TTGGAAATGC TCAACAAGCG CCTGGACCAC ATTGAGAAGA CCATTGACCC CTCCGGTCAC 
ATTTCGAGGA GAAGGAGCAT GTCGGCGTCG TCAAGAGGTG ACCACCACCA CCTTGGAGCC 
GTGACTGAGG AAGGGGGCGA CGAATCAGCC AATGAGACTG ATTCGGAAAC GGTGTCCACA 
GTCCCACAAA ACAAGCGCGA TGACCTGGTC AATCCCTACT GGATTGAAGA CCCCGATGTG 
AGAAAGGGCG AAGTGGAGTT CTTGAGCAGC ACCGAAATCC TCTTCTGGAA GGATCTACTC 
GATAAGTACT TGTACCCCAT TGACGAGAAC AAGGAGGAGA AGGCCCGTAT AGCCGCCGAC 
TTGAAGGAAT TGCGTGATCA ATCCGTGTTT GCTTTCTTTA TGATGAACGC TTTGTTCGTG 
CTGATAGTGT TCCTGCTGAC GTTGAAAAAG GACTACTTGC ACATAAAGTG GCCTTTCGGT 
GTAAAAACCA ACATAACCTA TGACGAGAGC ACCCAAGAGG TCCACATTAG CAAAGAGTAC 
CTCCAATTGG AACCGATCGG TCTAGTCTTT GTCTTCTTCT TCGCCCTTAT TTTGGTGATC 
CAGTTCGTTG CAATGTTGTT CCATCGATTC GGAACAATCG CCCACATTTT GGCCTCGACA 
GAGCTCAACT TGTGTTGCAC CAAGAAAAAG GAGGAATTGT CCCCGAACGC ACTACTCGAC 
AAACAAGCCG TGGAAATCGT CAAACAGCTG CAAAAACTGC AAGGCATTGA CGACGGCGAC 
TACGAGAACG ACTCGGGCTC AGGCCCGGAC CGCATCGGCC GCCGCAAAAC CATCCACAAC 
TTGGAGCGCG CGGCTCAGAA GAAGCGCCAA ATCGGAACTC TGGATGTGGC TTTCAAGAAG 
CGATTCGCTA AACTTAACGC CAATGGGACG AACGCCGGGA CTCCGGTCCT GAGTCGAAGA 
CTGACCATGA GGAGAGAGAC GATGAAAGCG CTCGAAGTCA GGGTGAACTC GGTGATGGCC 
GAAAGGCGCA AATCACACAT GCAAACACTA GGTGCCAAAA ATGAGTACGG GAATAATAAT 
GTGGTGGCGA GGAATCACAG GAACAGTGTC GCTAGCAGCA TTCCGGCCAA GGATGTGTTC 
GAAAATGGAC ATGTGAACAA GGCGTTCGAA GAGGACAATT ATGACCATCG CAGGAACTCG 
CTACAAATGC AACAAAGAAA CAACGTTACC TGGAAAAGCA GCAATAGCAG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum.
Journal of invertebrate pathology14326-34(2017 02)
Hayakawa Y,Kato D,Kamiya K,Minakuchi C,Miura K

Characterization of two chitin synthase genes of the red flour beetle, Tribolium castaneum, and alternate exon usage in one of the genes during development.
Insect biochemistry and molecular biology34(3)291-304(2004 Mar)
Arakane Y,Hogenkamp DG,Zhu YC,Kramer KJ,Specht CA,Beeman RW,Kanost MR,Muthukrishnan S

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Chs1 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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