LOC100142209 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100142209
Entrez Gene ID	100142209
Full Name	protein sidekick
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015983208.1	XP_015838694.1	protein sidekick isoform X2
XM_015983207.1	XP_015838693.1	protein sidekick isoform X1
XM_015983209.1	XP_015838695.1	protein sidekick isoform X6
XM_001811271.3	XP_001811323.1	protein sidekick isoform X4
XM_008199449.2	XP_008197671.1	protein sidekick isoform X3
XM_008199451.2	XP_008197673.1	protein sidekick isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC100142209 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100142209 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg12682	XM_008199449.2 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg12684	XM_001811271.3 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg12685	XM_008199451.2 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg21404	XM_015983207.1 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg21405	XM_015983208.1 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg21406	XM_015983209.1 Latest version!	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100142209 ( XM_008199449.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008197671.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X3, mRNA.

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CloneID	OTg12682
Clone ID Related Accession (Same CDS sequence)	XM_008199449.2
Accession Version	XM_008199449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008199449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGTTCCTCCG 
ATTTCAGGCC CAATTCCAGA TCCTTTACTC AATTGGGTAA CTGAACTCAG TAACGTATCA 
GCCGAACAAA GGTGGATTTT ATTAACAAAT TTAAAAGCAG CTGCTGCATA TCAGTTCCGA 
GTTAGCGCCG TTAATAGTGT GGGAGAAGGA AGTCCGTCCG AACCCAGTAA CCAAGTCATG 
TTACCTCAAG AAGCCCCTTC TGGACCTCCT GTTGGTTTCG TGGGTTCAGC CCGAAGTTCC 
TCCGAAATCA TAACACAGTG GCAGCCACCT CTCGAAGAGC ACCGTAACGG TCAAATCCTC 
GGTTACATAA TCAGATACAA ATTATTCGGT TACACCAACA GTCCTTGGAT CGTTCAAAAC 
ATAACAAACG AAGCGCAAAG AAATTACTTA ATTCAAGATT TAATAACTTG GAAAGATTAC 
ATCGTCCAGA TCGCCGCCTA CAACAACAAA GGCGTTGGGG TTTTCACCGA AGGCGCCCGA 
ATTAAGACCA AGGAGGGCGT CCCCGAAGCC CCCCCTATAA TTCAAACGGT GGTCCCCCTG 
AACTCCACCA CAATCCAAAT CACGTGGAAA CCGCCAGATC CGCAAAAAAT CAACGGAATC 
AACCAGGGAT ATAAAATCCA AGCGTGGCAA ACCGACAAAA ACGGCGACTT ATTTGAAGCC 
AAAATGATAA CAGTTCACCC GAATTTACTC GACCCGAACC AGGAACAAAC GGCGCTTATG 
ACGGGCCTAG AGAAGTTCAC CGACTACAAT GTTACGGTCC TGTGCTTCAC CGACCCTGGT 
GATGGCGAAA TCAGCGAGTT TGTCCCAGTG AAAACAAAAG AAGATGTACC TGACGAAGTC 
AGCAACTTGC AATTCGACGA CATTAGCGAC CGCGCCGTTA AAGTCATCTG GCAGCCCCCC 
AAGCACATCA ACGGCATCTT AACGGGGTAC CAAATCCGGT ACCAAATTCG CGACAAGCCC 
GACACTGTCA AAATCAAGAA CCTCACAGCC GATACCCTCA GCCTCAAAGT AACCGACTTG 
CAAGCCACCA CTCATTACAA ATTTGAGGTC ACGGCCTGGA CTGCAATGGG GGCAGGCCCC 
CCTAAAGTGG CGATCATCCA GTCGGGGGTG GAGCCCGTCT TGCCCCATCC CCCCAACAAA 
CTCGCCTTAT CCAACATTGA GGCCTTCTCT GTAGTTTTGC AATTCACCCC CGGCTTTGAC 
GGCAACTCCT CCATCACCAA GTGGACGGTG GAGGCCCAAA CTGCGCGCAA CGCCTCCTGG 
TTTGTCGTTT TCGAAATAAG CGATCCAGAC GCAACTACGA TTACTGTTAC GGGACTTGTC 
CCTTTCACTG TCTATAAACT GCGGTTGATT GCGAATAATG TCGTGGGGGC TTCCGAGCCC 
TCCGAAGCCT CCAAGGAGTT CCAGACGATC CAAGCGCCCC CAGCACACCC CCCGAAGAAC 
GTCACAGTGC GGGCCATGAG CGCGACTGAA GTGAGGGTTC GCTGGATTCC TCTGCAGCAA 
ATCGAATGGT TCGGGAACCC GCGAGGGTAC TCGATTAGTT ACGTGGAGGT GCGAACGGGG 
GAAAAACAGA CGGCGACAAT TGACGATCAT ACGGCCAATT CGCATGTGAT TACCGGATTG 
GAGGAGTTTG CGTTGTATGA AGTGTCCATG CAGGCGTGCA ATGACGTGGG GTGTTCCATG 
GACGGGCCCG TGGCTTTGGA GAGGACCAGG GAGTCAGTAC CATCGTTCGG GCCGTTAAAC 
ATCGAAGCGA ACGCTACCTC TAGCACAACA ATCGTAGTGA AATGGGGTGA CGTCCCGAAA 
GAACACCAAA ACGGCCTAAT TGAAGGCTAC AAAGTTTATT ACGCCGCCGA ATCAAAATCG 
CCCGTACAGT ACAAGCTAAT CGCCAGCAAT TCCACTTTTA CAACCACTTT GACCGAATTG 
CGGAAATTCG TCGTTTACCG CATCCAGGTC CTGGCGTACA CACGGCTCGG CGACGGCGAG 
CCCAGCAAAC CGGCCGTCAG CGTGCGAACC TTCGAAGATG TCCCGGGCCC CCCCTCGAAT 
GTGTCATTCC CGGACGTTTC CGTCACTTCG GCCAGGATCA TCTGGGACGT CCCGGAAGAA 
CCCAACGGCG AAATCCTGGC GTACAAAGTC ACATATCATA TCGACAACGT GAACGTTAAT 
AATAAATACT CGAAGGAATT TCCGCCGTCT GATAGAACGT TCAGGTTTAC GCAGTTGGAG 
CCTGAGAAGT ATTACATGTT TTCTGTGACT GCTCAGACCA GATTAGGTTG GGGCAAGACA 
GCTCATGCTT TGGTTTTTAC AACGAATAAT CGAGACACAC CACAGTCACC ATCTATGCCA 
CAAATCAGTC GCAGTCAGGT ACAGAGCAAG CAGATCACTT TCAGCTGGAC ACCGGGCAGA 
GACGGATTCG CACCGTTGAG ATATTATACG GTCCAAAAAA TGGAAAATGG GGGCCCTTGG 
CAGTCCCTTC CCGAACGTGT GGACCCTCAA CTCACCTCAT ACACCGTCTC TGACCTCAAA 
CCCTTCACTT CATACCGCTT CAGAATCCGC GCAACGAACG ATATAGGTCC CAGCCCCTTC 
AGTCCCGAAT CCATCGAAGT CCGCACTTAT CCAGCAGCCC CCAGCAAAGG CGTCATGAAC 
TTAAAAGTAG TTCCCATAAC AACGACAAGT GTCGAAGTCT ATTGGGACCC AGTTGAGGAG 
AAGTACTGGA ACGGGGACGT CAAAACAGGG GGCTATAGAG TAGTCTTTCA ACCCGTCTCT 
GACTATCCAA CTGCACTGCA GGCAACACCC AAGGAGGAAA TTTTCGGAAT CAATGCCAAA 
AAAATGGTTC TGAGCGAATT ATTGCAAGAC AAGAATTATG AGATAGTTGT GGTGCCGTTT 
AATTCGCAAG GGGAGGGCCC CGCAACGCCC CCTGTCACAG TGTATGTAGG GGAAGCCGTC 
CCAACTGGTG AACCAAGGGA CTTGGAGGGC GAAGCTGTTT CCTCGACGGA AGTTCGCTTG 
AAATGGAAGC CGCCACAGCA GCAGCAACAA AACGGCGAAT TGTTGGGATA CAAGATATTT 
TATTTGGTGA CAAACTCGCC GCAAGATTTT GACGAGGGCC ACAAGTTCGA GGAGGAAATC 
GAAGTGATTG CAGCTACGAT GACCTCGCAC AGCCTTGTCT TTCTCGACAA ATACACCGAA 
TATCGCATAC AAATTCTGGC GTTTAACCCA GCAGGTGATG GACCACGATC GCGCCCCATC 
ACAGTTAAGA CCCTTCAAGG TCTCCCTGGT CCACCAGCCA ATCTACGCTT TTCTGACATA 
ACGATGAATA GCTTGCTCGT GTCGTGGGAT CCCCCGAAAA AACGCAACGG TGACATAATC 
GGATACCTTG TCACGTATGA GACGACCGAG GAGAACGAAC GTTTTAGTAA ACAAGTCAAG 
CAGAAAGTGA CGTCGACGGA ACTCTTGCTA CAAACTTTGG AGGAGGAAAT CACTTACACG 
TTCACAGTGC GGGCACAAAC CATCGATTAT GGGCCCTCTA TTTCGGATAA CGTCACCACA 
GGGCCACAGG ACGGCTCCCC AACGACGCCA AAAGAACTCA CTTTGATTAA AACACCGTCA 
AGTGTGGAGC TGCACTGGGT CAATGGCTTG ACCGGAAAGG GGCCAATTCT GGGCTACTAT 
ATCGAATCGA GGAAAAAAGA TGACCAGAGG TGGCAAACAG TCACGAAATC GACAAACGGG 
CCTTTGCAAG AATTTACAGT TTCGTACCAA AGTCTCCTCC CCTCAACCTC TTACTATTTC 
AGAGTTTTTC CTTACAATAA ATTCGGTATT AGTTGTCCCG CGTACACCAA AGACCCGGTC 
GTAACACCGT CAAAACTGTA CTTGGAATAT AGCTACTTAC AGTATGAACC CTTCTACCGA 
CAGACCTGGT TCATGGTCGC CCTGGCAGCG ACCTCCATCA TCATTATCAT AATGATAATT 
GCGATTTTGT GCGTTAAGAG CAAAAGTTAC AAATATAAAC AAGAAGGTGC CCAAAATAGT 
ATGGGCGAAC TCATGGCAAT GTCACGCGAC GACATGACCG CTGACTATTA CCGAACTCGC 
AACGGCACAA GTAACCTAAG CTCGACTCTT TCAAAACGTA GCACTTTATC CCGCAAGCCG 
GTCCATCAAC AACCCCCTCC AATGTTGGGG AAATCCCCTC CGAGACCATC GCCGGCTTCA 
GTGCCCTACA ACTCAGACGA GGAAAGTCTC AAGGGCTACG ATGAGAATCC CGACGATAGC 
AGCGTGACCG AGAAGCCGTC AGAAATGAGC TCTTCTGAAT CCCAGGGTTC GGAAAGCGAA 
AACGACAGTG TCCTCTCGAA TTCGCACTCG TTTGTGAACC ACTACGCCAA TGTGAACGAG 
CCATTGCGAC CGTCCTGGAA GCGCCAAAAG CCGGTCAGGA ATTTCTCGAG TCACACTGAT 
TCGGAACCGG AAGGTTCGGC GAGTTTGAAT GGAGGACAGA TTATCATTAA TAACATGGCG 
AGGTCAAGGG CGCCCCTACC CGGATTTTCG TCGTTTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008197671.1
CDS	385..6906
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKTAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELVPPISGPIPDPLLNWVTELSNVSAEQRWILLTNLKAAAAYQFRVSAVNSVGEGSPSEPSNQVMLPQEAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQEGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_008199449.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008199449.2

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGTTCCTCCG 
ATTTCAGGCC CAATTCCAGA TCCTTTACTC AATTGGGTAA CTGAACTCAG TAACGTATCA 
GCCGAACAAA GGTGGATTTT ATTAACAAAT TTAAAAGCAG CTGCTGCATA TCAGTTCCGA 
GTTAGCGCCG TTAATAGTGT GGGAGAAGGA AGTCCGTCCG AACCCAGTAA CCAAGTCATG 
TTACCTCAAG AAGCCCCTTC TGGACCTCCT GTTGGTTTCG TGGGTTCAGC CCGAAGTTCC 
TCCGAAATCA TAACACAGTG GCAGCCACCT CTCGAAGAGC ACCGTAACGG TCAAATCCTC 
GGTTACATAA TCAGATACAA ATTATTCGGT TACACCAACA GTCCTTGGAT CGTTCAAAAC 
ATAACAAACG AAGCGCAAAG AAATTACTTA ATTCAAGATT TAATAACTTG GAAAGATTAC 
ATCGTCCAGA TCGCCGCCTA CAACAACAAA GGCGTTGGGG TTTTCACCGA AGGCGCCCGA 
ATTAAGACCA AGGAGGGCGT CCCCGAAGCC CCCCCTATAA TTCAAACGGT GGTCCCCCTG 
AACTCCACCA CAATCCAAAT CACGTGGAAA CCGCCAGATC CGCAAAAAAT CAACGGAATC 
AACCAGGGAT ATAAAATCCA AGCGTGGCAA ACCGACAAAA ACGGCGACTT ATTTGAAGCC 
AAAATGATAA CAGTTCACCC GAATTTACTC GACCCGAACC AGGAACAAAC GGCGCTTATG 
ACGGGCCTAG AGAAGTTCAC CGACTACAAT GTTACGGTCC TGTGCTTCAC CGACCCTGGT 
GATGGCGAAA TCAGCGAGTT TGTCCCAGTG AAAACAAAAG AAGATGTACC TGACGAAGTC 
AGCAACTTGC AATTCGACGA CATTAGCGAC CGCGCCGTTA AAGTCATCTG GCAGCCCCCC 
AAGCACATCA ACGGCATCTT AACGGGGTAC CAAATCCGGT ACCAAATTCG CGACAAGCCC 
GACACTGTCA AAATCAAGAA CCTCACAGCC GATACCCTCA GCCTCAAAGT AACCGACTTG 
CAAGCCACCA CTCATTACAA ATTTGAGGTC ACGGCCTGGA CTGCAATGGG GGCAGGCCCC 
CCTAAAGTGG CGATCATCCA GTCGGGGGTG GAGCCCGTCT TGCCCCATCC CCCCAACAAA 
CTCGCCTTAT CCAACATTGA GGCCTTCTCT GTAGTTTTGC AATTCACCCC CGGCTTTGAC 
GGCAACTCCT CCATCACCAA GTGGACGGTG GAGGCCCAAA CTGCGCGCAA CGCCTCCTGG 
TTTGTCGTTT TCGAAATAAG CGATCCAGAC GCAACTACGA TTACTGTTAC GGGACTTGTC 
CCTTTCACTG TCTATAAACT GCGGTTGATT GCGAATAATG TCGTGGGGGC TTCCGAGCCC 
TCCGAAGCCT CCAAGGAGTT CCAGACGATC CAAGCGCCCC CAGCACACCC CCCGAAGAAC 
GTCACAGTGC GGGCCATGAG CGCGACTGAA GTGAGGGTTC GCTGGATTCC TCTGCAGCAA 
ATCGAATGGT TCGGGAACCC GCGAGGGTAC TCGATTAGTT ACGTGGAGGT GCGAACGGGG 
GAAAAACAGA CGGCGACAAT TGACGATCAT ACGGCCAATT CGCATGTGAT TACCGGATTG 
GAGGAGTTTG CGTTGTATGA AGTGTCCATG CAGGCGTGCA ATGACGTGGG GTGTTCCATG 
GACGGGCCCG TGGCTTTGGA GAGGACCAGG GAGTCAGTAC CATCGTTCGG GCCGTTAAAC 
ATCGAAGCGA ACGCTACCTC TAGCACAACA ATCGTAGTGA AATGGGGTGA CGTCCCGAAA 
GAACACCAAA ACGGCCTAAT TGAAGGCTAC AAAGTTTATT ACGCCGCCGA ATCAAAATCG 
CCCGTACAGT ACAAGCTAAT CGCCAGCAAT TCCACTTTTA CAACCACTTT GACCGAATTG 
CGGAAATTCG TCGTTTACCG CATCCAGGTC CTGGCGTACA CACGGCTCGG CGACGGCGAG 
CCCAGCAAAC CGGCCGTCAG CGTGCGAACC TTCGAAGATG TCCCGGGCCC CCCCTCGAAT 
GTGTCATTCC CGGACGTTTC CGTCACTTCG GCCAGGATCA TCTGGGACGT CCCGGAAGAA 
CCCAACGGCG AAATCCTGGC GTACAAAGTC ACATATCATA TCGACAACGT GAACGTTAAT 
AATAAATACT CGAAGGAATT TCCGCCGTCT GATAGAACGT TCAGGTTTAC GCAGTTGGAG 
CCTGAGAAGT ATTACATGTT TTCTGTGACT GCTCAGACCA GATTAGGTTG GGGCAAGACA 
GCTCATGCTT TGGTTTTTAC AACGAATAAT CGAGACACAC CACAGTCACC ATCTATGCCA 
CAAATCAGTC GCAGTCAGGT ACAGAGCAAG CAGATCACTT TCAGCTGGAC ACCGGGCAGA 
GACGGATTCG CACCGTTGAG ATATTATACG GTCCAAAAAA TGGAAAATGG GGGCCCTTGG 
CAGTCCCTTC CCGAACGTGT GGACCCTCAA CTCACCTCAT ACACCGTCTC TGACCTCAAA 
CCCTTCACTT CATACCGCTT CAGAATCCGC GCAACGAACG ATATAGGTCC CAGCCCCTTC 
AGTCCCGAAT CCATCGAAGT CCGCACTTAT CCAGCAGCCC CCAGCAAAGG CGTCATGAAC 
TTAAAAGTAG TTCCCATAAC AACGACAAGT GTCGAAGTCT ATTGGGACCC AGTTGAGGAG 
AAGTACTGGA ACGGGGACGT CAAAACAGGG GGCTATAGAG TAGTCTTTCA ACCCGTCTCT 
GACTATCCAA CTGCACTGCA GGCAACACCC AAGGAGGAAA TTTTCGGAAT CAATGCCAAA 
AAAATGGTTC TGAGCGAATT ATTGCAAGAC AAGAATTATG AGATAGTTGT GGTGCCGTTT 
AATTCGCAAG GGGAGGGCCC CGCAACGCCC CCTGTCACAG TGTATGTAGG GGAAGCCGTC 
CCAACTGGTG AACCAAGGGA CTTGGAGGGC GAAGCTGTTT CCTCGACGGA AGTTCGCTTG 
AAATGGAAGC CGCCACAGCA GCAGCAACAA AACGGCGAAT TGTTGGGATA CAAGATATTT 
TATTTGGTGA CAAACTCGCC GCAAGATTTT GACGAGGGCC ACAAGTTCGA GGAGGAAATC 
GAAGTGATTG CAGCTACGAT GACCTCGCAC AGCCTTGTCT TTCTCGACAA ATACACCGAA 
TATCGCATAC AAATTCTGGC GTTTAACCCA GCAGGTGATG GACCACGATC GCGCCCCATC 
ACAGTTAAGA CCCTTCAAGG TCTCCCTGGT CCACCAGCCA ATCTACGCTT TTCTGACATA 
ACGATGAATA GCTTGCTCGT GTCGTGGGAT CCCCCGAAAA AACGCAACGG TGACATAATC 
GGATACCTTG TCACGTATGA GACGACCGAG GAGAACGAAC GTTTTAGTAA ACAAGTCAAG 
CAGAAAGTGA CGTCGACGGA ACTCTTGCTA CAAACTTTGG AGGAGGAAAT CACTTACACG 
TTCACAGTGC GGGCACAAAC CATCGATTAT GGGCCCTCTA TTTCGGATAA CGTCACCACA 
GGGCCACAGG ACGGCTCCCC AACGACGCCA AAAGAACTCA CTTTGATTAA AACACCGTCA 
AGTGTGGAGC TGCACTGGGT CAATGGCTTG ACCGGAAAGG GGCCAATTCT GGGCTACTAT 
ATCGAATCGA GGAAAAAAGA TGACCAGAGG TGGCAAACAG TCACGAAATC GACAAACGGG 
CCTTTGCAAG AATTTACAGT TTCGTACCAA AGTCTCCTCC CCTCAACCTC TTACTATTTC 
AGAGTTTTTC CTTACAATAA ATTCGGTATT AGTTGTCCCG CGTACACCAA AGACCCGGTC 
GTAACACCGT CAAAACTGTA CTTGGAATAT AGCTACTTAC AGTATGAACC CTTCTACCGA 
CAGACCTGGT TCATGGTCGC CCTGGCAGCG ACCTCCATCA TCATTATCAT AATGATAATT 
GCGATTTTGT GCGTTAAGAG CAAAAGTTAC AAATATAAAC AAGAAGGTGC CCAAAATAGT 
ATGGGCGAAC TCATGGCAAT GTCACGCGAC GACATGACCG CTGACTATTA CCGAACTCGC 
AACGGCACAA GTAACCTAAG CTCGACTCTT TCAAAACGTA GCACTTTATC CCGCAAGCCG 
GTCCATCAAC AACCCCCTCC AATGTTGGGG AAATCCCCTC CGAGACCATC GCCGGCTTCA 
GTGCCCTACA ACTCAGACGA GGAAAGTCTC AAGGGCTACG ATGAGAATCC CGACGATAGC 
AGCGTGACCG AGAAGCCGTC AGAAATGAGC TCTTCTGAAT CCCAGGGTTC GGAAAGCGAA 
AACGACAGTG TCCTCTCGAA TTCGCACTCG TTTGTGAACC ACTACGCCAA TGTGAACGAG 
CCATTGCGAC CGTCCTGGAA GCGCCAAAAG CCGGTCAGGA ATTTCTCGAG TCACACTGAT 
TCGGAACCGG AAGGTTCGGC GAGTTTGAAT GGAGGACAGA TTATCATTAA TAACATGGCG 
AGGTCAAGGG CGCCCCTACC CGGATTTTCG TCGTTTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100142209 ( XM_001811271.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_001811323.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X4, mRNA.

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CloneID	OTg12684
Clone ID Related Accession (Same CDS sequence)	XM_001811271.3
Accession Version	XM_001811271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_001811271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGGCCCAATT 
CCAGATCCTT TACTCAATTG GGTAACTGAA CTCAGTAACG TATCAGCCGA ACAAAGGTGG 
ATTTTATTAA CAAATTTAAA AGCAGCTGCT GCATATCAGT TCCGAGTTAG CGCCGTTAAT 
AGTGTGGGAG AAGGAAGTCC GTCCGAACCC AGTAACCAAG TCATGTTACC TCAAGAAGCC 
CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT TCAGCCCGAA GTTCCTCCGA AATCATAACA 
CAGTGGCAGC CACCTCTCGA AGAGCACCGT AACGGTCAAA TCCTCGGTTA CATAATCAGA 
TACAAATTAT TCGGTTACAC CAACAGTCCT TGGATCGTTC AAAACATAAC AAACGAAGCG 
CAAAGAAATT ACTTAATTCA AGATTTAATA ACTTGGAAAG ATTACATCGT CCAGATCGCC 
GCCTACAACA ACAAAGGCGT TGGGGTTTTC ACCGAAGGCG CCCGAATTAA GACCAAGGAG 
GGCGTCCCCG AAGCCCCCCC TATAATTCAA ACGGTGGTCC CCCTGAACTC CACCACAATC 
CAAATCACGT GGAAACCGCC AGATCCGCAA AAAATCAACG GAATCAACCA GGGATATAAA 
ATCCAAGCGT GGCAAACCGA CAAAAACGGC GACTTATTTG AAGCCAAAAT GATAACAGTT 
CACCCGAATT TACTCGACCC GAACCAGGAA CAAACGGCGC TTATGACGGG CCTAGAGAAG 
TTCACCGACT ACAATGTTAC GGTCCTGTGC TTCACCGACC CTGGTGATGG CGAAATCAGC 
GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT GTACCTGACG AAGTCAGCAA CTTGCAATTC 
GACGACATTA GCGACCGCGC CGTTAAAGTC ATCTGGCAGC CCCCCAAGCA CATCAACGGC 
ATCTTAACGG GGTACCAAAT CCGGTACCAA ATTCGCGACA AGCCCGACAC TGTCAAAATC 
AAGAACCTCA CAGCCGATAC CCTCAGCCTC AAAGTAACCG ACTTGCAAGC CACCACTCAT 
TACAAATTTG AGGTCACGGC CTGGACTGCA ATGGGGGCAG GCCCCCCTAA AGTGGCGATC 
ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC CATCCCCCCA ACAAACTCGC CTTATCCAAC 
ATTGAGGCCT TCTCTGTAGT TTTGCAATTC ACCCCCGGCT TTGACGGCAA CTCCTCCATC 
ACCAAGTGGA CGGTGGAGGC CCAAACTGCG CGCAACGCCT CCTGGTTTGT CGTTTTCGAA 
ATAAGCGATC CAGACGCAAC TACGATTACT GTTACGGGAC TTGTCCCTTT CACTGTCTAT 
AAACTGCGGT TGATTGCGAA TAATGTCGTG GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG 
GAGTTCCAGA CGATCCAAGC GCCCCCAGCA CACCCCCCGA AGAACGTCAC AGTGCGGGCC 
ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG ATTCCTCTGC AGCAAATCGA ATGGTTCGGG 
AACCCGCGAG GGTACTCGAT TAGTTACGTG GAGGTGCGAA CGGGGGAAAA ACAGACGGCG 
ACAATTGACG ATCATACGGC CAATTCGCAT GTGATTACCG GATTGGAGGA GTTTGCGTTG 
TATGAAGTGT CCATGCAGGC GTGCAATGAC GTGGGGTGTT CCATGGACGG GCCCGTGGCT 
TTGGAGAGGA CCAGGGAGTC AGTACCATCG TTCGGGCCGT TAAACATCGA AGCGAACGCT 
ACCTCTAGCA CAACAATCGT AGTGAAATGG GGTGACGTCC CGAAAGAACA CCAAAACGGC 
CTAATTGAAG GCTACAAAGT TTATTACGCC GCCGAATCAA AATCGCCCGT ACAGTACAAG 
CTAATCGCCA GCAATTCCAC TTTTACAACC ACTTTGACCG AATTGCGGAA ATTCGTCGTT 
TACCGCATCC AGGTCCTGGC GTACACACGG CTCGGCGACG GCGAGCCCAG CAAACCGGCC 
GTCAGCGTGC GAACCTTCGA AGATGTCCCG GGCCCCCCCT CGAATGTGTC ATTCCCGGAC 
GTTTCCGTCA CTTCGGCCAG GATCATCTGG GACGTCCCGG AAGAACCCAA CGGCGAAATC 
CTGGCGTACA AAGTCACATA TCATATCGAC AACGTGAACG TTAATAATAA ATACTCGAAG 
GAATTTCCGC CGTCTGATAG AACGTTCAGG TTTACGCAGT TGGAGCCTGA GAAGTATTAC 
ATGTTTTCTG TGACTGCTCA GACCAGATTA GGTTGGGGCA AGACAGCTCA TGCTTTGGTT 
TTTACAACGA ATAATCGAGA CACACCACAG TCACCATCTA TGCCACAAAT CAGTCGCAGT 
CAGGTACAGA GCAAGCAGAT CACTTTCAGC TGGACACCGG GCAGAGACGG ATTCGCACCG 
TTGAGATATT ATACGGTCCA AAAAATGGAA AATGGGGGCC CTTGGCAGTC CCTTCCCGAA 
CGTGTGGACC CTCAACTCAC CTCATACACC GTCTCTGACC TCAAACCCTT CACTTCATAC 
CGCTTCAGAA TCCGCGCAAC GAACGATATA GGTCCCAGCC CCTTCAGTCC CGAATCCATC 
GAAGTCCGCA CTTATCCAGC AGCCCCCAGC AAAGGCGTCA TGAACTTAAA AGTAGTTCCC 
ATAACAACGA CAAGTGTCGA AGTCTATTGG GACCCAGTTG AGGAGAAGTA CTGGAACGGG 
GACGTCAAAA CAGGGGGCTA TAGAGTAGTC TTTCAACCCG TCTCTGACTA TCCAACTGCA 
CTGCAGGCAA CACCCAAGGA GGAAATTTTC GGAATCAATG CCAAAAAAAT GGTTCTGAGC 
GAATTATTGC AAGACAAGAA TTATGAGATA GTTGTGGTGC CGTTTAATTC GCAAGGGGAG 
GGCCCCGCAA CGCCCCCTGT CACAGTGTAT GTAGGGGAAG CCGTCCCAAC TGGTGAACCA 
AGGGACTTGG AGGGCGAAGC TGTTTCCTCG ACGGAAGTTC GCTTGAAATG GAAGCCGCCA 
CAGCAGCAGC AACAAAACGG CGAATTGTTG GGATACAAGA TATTTTATTT GGTGACAAAC 
TCGCCGCAAG ATTTTGACGA GGGCCACAAG TTCGAGGAGG AAATCGAAGT GATTGCAGCT 
ACGATGACCT CGCACAGCCT TGTCTTTCTC GACAAATACA CCGAATATCG CATACAAATT 
CTGGCGTTTA ACCCAGCAGG TGATGGACCA CGATCGCGCC CCATCACAGT TAAGACCCTT 
CAAGGTCTCC CTGGTCCACC AGCCAATCTA CGCTTTTCTG ACATAACGAT GAATAGCTTG 
CTCGTGTCGT GGGATCCCCC GAAAAAACGC AACGGTGACA TAATCGGATA CCTTGTCACG 
TATGAGACGA CCGAGGAGAA CGAACGTTTT AGTAAACAAG TCAAGCAGAA AGTGACGTCG 
ACGGAACTCT TGCTACAAAC TTTGGAGGAG GAAATCACTT ACACGTTCAC AGTGCGGGCA 
CAAACCATCG ATTATGGGCC CTCTATTTCG GATAACGTCA CCACAGGGCC ACAGGACGGC 
TCCCCAACGA CGCCAAAAGA ACTCACTTTG ATTAAAACAC CGTCAAGTGT GGAGCTGCAC 
TGGGTCAATG GCTTGACCGG AAAGGGGCCA ATTCTGGGCT ACTATATCGA ATCGAGGAAA 
AAAGATGACC AGAGGTGGCA AACAGTCACG AAATCGACAA ACGGGCCTTT GCAAGAATTT 
ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA ACCTCTTACT ATTTCAGAGT TTTTCCTTAC 
AATAAATTCG GTATTAGTTG TCCCGCGTAC ACCAAAGACC CGGTCGTAAC ACCGTCAAAA 
CTGTACTTGG AATATAGCTA CTTACAGTAT GAACCCTTCT ACCGACAGAC CTGGTTCATG 
GTCGCCCTGG CAGCGACCTC CATCATCATT ATCATAATGA TAATTGCGAT TTTGTGCGTT 
AAGAGCAAAA GTTACAAATA TAAACAAGAA GGTGCCCAAA ATAGTATGGG CGAACTCATG 
GCAATGTCAC GCGACGACAT GACCGCTGAC TATTACCGAA CTCGCAACGG CACAAGTAAC 
CTAAGCTCGA CTCTTTCAAA ACGTAGCACT TTATCCCGCA AGCCGGTCCA TCAACAACCC 
CCTCCAATGT TGGGGAAATC CCCTCCGAGA CCATCGCCGG CTTCAGTGCC CTACAACTCA 
GACGAGGAAA GTCTCAAGGG CTACGATGAG AATCCCGACG ATAGCAGCGT GACCGAGAAG 
CCGTCAGAAA TGAGCTCTTC TGAATCCCAG GGTTCGGAAA GCGAAAACGA CAGTGTCCTC 
TCGAATTCGC ACTCGTTTGT GAACCACTAC GCCAATGTGA ACGAGCCATT GCGACCGTCC 
TGGAAGCGCC AAAAGCCGGT CAGGAATTTC TCGAGTCACA CTGATTCGGA ACCGGAAGGT 
TCGGCGAGTT TGAATGGAGG ACAGATTATC ATTAATAACA TGGCGAGGTC AAGGGCGCCC 
CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001811323.1
CDS	385..6891
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKTAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELGPIPDPLLNWVTELSNVSAEQRWILLTNLKAAAAYQFRVSAVNSVGEGSPSEPSNQVMLPQEAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQEGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_001811271.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001811271.3

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGGCCCAATT 
CCAGATCCTT TACTCAATTG GGTAACTGAA CTCAGTAACG TATCAGCCGA ACAAAGGTGG 
ATTTTATTAA CAAATTTAAA AGCAGCTGCT GCATATCAGT TCCGAGTTAG CGCCGTTAAT 
AGTGTGGGAG AAGGAAGTCC GTCCGAACCC AGTAACCAAG TCATGTTACC TCAAGAAGCC 
CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT TCAGCCCGAA GTTCCTCCGA AATCATAACA 
CAGTGGCAGC CACCTCTCGA AGAGCACCGT AACGGTCAAA TCCTCGGTTA CATAATCAGA 
TACAAATTAT TCGGTTACAC CAACAGTCCT TGGATCGTTC AAAACATAAC AAACGAAGCG 
CAAAGAAATT ACTTAATTCA AGATTTAATA ACTTGGAAAG ATTACATCGT CCAGATCGCC 
GCCTACAACA ACAAAGGCGT TGGGGTTTTC ACCGAAGGCG CCCGAATTAA GACCAAGGAG 
GGCGTCCCCG AAGCCCCCCC TATAATTCAA ACGGTGGTCC CCCTGAACTC CACCACAATC 
CAAATCACGT GGAAACCGCC AGATCCGCAA AAAATCAACG GAATCAACCA GGGATATAAA 
ATCCAAGCGT GGCAAACCGA CAAAAACGGC GACTTATTTG AAGCCAAAAT GATAACAGTT 
CACCCGAATT TACTCGACCC GAACCAGGAA CAAACGGCGC TTATGACGGG CCTAGAGAAG 
TTCACCGACT ACAATGTTAC GGTCCTGTGC TTCACCGACC CTGGTGATGG CGAAATCAGC 
GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT GTACCTGACG AAGTCAGCAA CTTGCAATTC 
GACGACATTA GCGACCGCGC CGTTAAAGTC ATCTGGCAGC CCCCCAAGCA CATCAACGGC 
ATCTTAACGG GGTACCAAAT CCGGTACCAA ATTCGCGACA AGCCCGACAC TGTCAAAATC 
AAGAACCTCA CAGCCGATAC CCTCAGCCTC AAAGTAACCG ACTTGCAAGC CACCACTCAT 
TACAAATTTG AGGTCACGGC CTGGACTGCA ATGGGGGCAG GCCCCCCTAA AGTGGCGATC 
ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC CATCCCCCCA ACAAACTCGC CTTATCCAAC 
ATTGAGGCCT TCTCTGTAGT TTTGCAATTC ACCCCCGGCT TTGACGGCAA CTCCTCCATC 
ACCAAGTGGA CGGTGGAGGC CCAAACTGCG CGCAACGCCT CCTGGTTTGT CGTTTTCGAA 
ATAAGCGATC CAGACGCAAC TACGATTACT GTTACGGGAC TTGTCCCTTT CACTGTCTAT 
AAACTGCGGT TGATTGCGAA TAATGTCGTG GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG 
GAGTTCCAGA CGATCCAAGC GCCCCCAGCA CACCCCCCGA AGAACGTCAC AGTGCGGGCC 
ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG ATTCCTCTGC AGCAAATCGA ATGGTTCGGG 
AACCCGCGAG GGTACTCGAT TAGTTACGTG GAGGTGCGAA CGGGGGAAAA ACAGACGGCG 
ACAATTGACG ATCATACGGC CAATTCGCAT GTGATTACCG GATTGGAGGA GTTTGCGTTG 
TATGAAGTGT CCATGCAGGC GTGCAATGAC GTGGGGTGTT CCATGGACGG GCCCGTGGCT 
TTGGAGAGGA CCAGGGAGTC AGTACCATCG TTCGGGCCGT TAAACATCGA AGCGAACGCT 
ACCTCTAGCA CAACAATCGT AGTGAAATGG GGTGACGTCC CGAAAGAACA CCAAAACGGC 
CTAATTGAAG GCTACAAAGT TTATTACGCC GCCGAATCAA AATCGCCCGT ACAGTACAAG 
CTAATCGCCA GCAATTCCAC TTTTACAACC ACTTTGACCG AATTGCGGAA ATTCGTCGTT 
TACCGCATCC AGGTCCTGGC GTACACACGG CTCGGCGACG GCGAGCCCAG CAAACCGGCC 
GTCAGCGTGC GAACCTTCGA AGATGTCCCG GGCCCCCCCT CGAATGTGTC ATTCCCGGAC 
GTTTCCGTCA CTTCGGCCAG GATCATCTGG GACGTCCCGG AAGAACCCAA CGGCGAAATC 
CTGGCGTACA AAGTCACATA TCATATCGAC AACGTGAACG TTAATAATAA ATACTCGAAG 
GAATTTCCGC CGTCTGATAG AACGTTCAGG TTTACGCAGT TGGAGCCTGA GAAGTATTAC 
ATGTTTTCTG TGACTGCTCA GACCAGATTA GGTTGGGGCA AGACAGCTCA TGCTTTGGTT 
TTTACAACGA ATAATCGAGA CACACCACAG TCACCATCTA TGCCACAAAT CAGTCGCAGT 
CAGGTACAGA GCAAGCAGAT CACTTTCAGC TGGACACCGG GCAGAGACGG ATTCGCACCG 
TTGAGATATT ATACGGTCCA AAAAATGGAA AATGGGGGCC CTTGGCAGTC CCTTCCCGAA 
CGTGTGGACC CTCAACTCAC CTCATACACC GTCTCTGACC TCAAACCCTT CACTTCATAC 
CGCTTCAGAA TCCGCGCAAC GAACGATATA GGTCCCAGCC CCTTCAGTCC CGAATCCATC 
GAAGTCCGCA CTTATCCAGC AGCCCCCAGC AAAGGCGTCA TGAACTTAAA AGTAGTTCCC 
ATAACAACGA CAAGTGTCGA AGTCTATTGG GACCCAGTTG AGGAGAAGTA CTGGAACGGG 
GACGTCAAAA CAGGGGGCTA TAGAGTAGTC TTTCAACCCG TCTCTGACTA TCCAACTGCA 
CTGCAGGCAA CACCCAAGGA GGAAATTTTC GGAATCAATG CCAAAAAAAT GGTTCTGAGC 
GAATTATTGC AAGACAAGAA TTATGAGATA GTTGTGGTGC CGTTTAATTC GCAAGGGGAG 
GGCCCCGCAA CGCCCCCTGT CACAGTGTAT GTAGGGGAAG CCGTCCCAAC TGGTGAACCA 
AGGGACTTGG AGGGCGAAGC TGTTTCCTCG ACGGAAGTTC GCTTGAAATG GAAGCCGCCA 
CAGCAGCAGC AACAAAACGG CGAATTGTTG GGATACAAGA TATTTTATTT GGTGACAAAC 
TCGCCGCAAG ATTTTGACGA GGGCCACAAG TTCGAGGAGG AAATCGAAGT GATTGCAGCT 
ACGATGACCT CGCACAGCCT TGTCTTTCTC GACAAATACA CCGAATATCG CATACAAATT 
CTGGCGTTTA ACCCAGCAGG TGATGGACCA CGATCGCGCC CCATCACAGT TAAGACCCTT 
CAAGGTCTCC CTGGTCCACC AGCCAATCTA CGCTTTTCTG ACATAACGAT GAATAGCTTG 
CTCGTGTCGT GGGATCCCCC GAAAAAACGC AACGGTGACA TAATCGGATA CCTTGTCACG 
TATGAGACGA CCGAGGAGAA CGAACGTTTT AGTAAACAAG TCAAGCAGAA AGTGACGTCG 
ACGGAACTCT TGCTACAAAC TTTGGAGGAG GAAATCACTT ACACGTTCAC AGTGCGGGCA 
CAAACCATCG ATTATGGGCC CTCTATTTCG GATAACGTCA CCACAGGGCC ACAGGACGGC 
TCCCCAACGA CGCCAAAAGA ACTCACTTTG ATTAAAACAC CGTCAAGTGT GGAGCTGCAC 
TGGGTCAATG GCTTGACCGG AAAGGGGCCA ATTCTGGGCT ACTATATCGA ATCGAGGAAA 
AAAGATGACC AGAGGTGGCA AACAGTCACG AAATCGACAA ACGGGCCTTT GCAAGAATTT 
ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA ACCTCTTACT ATTTCAGAGT TTTTCCTTAC 
AATAAATTCG GTATTAGTTG TCCCGCGTAC ACCAAAGACC CGGTCGTAAC ACCGTCAAAA 
CTGTACTTGG AATATAGCTA CTTACAGTAT GAACCCTTCT ACCGACAGAC CTGGTTCATG 
GTCGCCCTGG CAGCGACCTC CATCATCATT ATCATAATGA TAATTGCGAT TTTGTGCGTT 
AAGAGCAAAA GTTACAAATA TAAACAAGAA GGTGCCCAAA ATAGTATGGG CGAACTCATG 
GCAATGTCAC GCGACGACAT GACCGCTGAC TATTACCGAA CTCGCAACGG CACAAGTAAC 
CTAAGCTCGA CTCTTTCAAA ACGTAGCACT TTATCCCGCA AGCCGGTCCA TCAACAACCC 
CCTCCAATGT TGGGGAAATC CCCTCCGAGA CCATCGCCGG CTTCAGTGCC CTACAACTCA 
GACGAGGAAA GTCTCAAGGG CTACGATGAG AATCCCGACG ATAGCAGCGT GACCGAGAAG 
CCGTCAGAAA TGAGCTCTTC TGAATCCCAG GGTTCGGAAA GCGAAAACGA CAGTGTCCTC 
TCGAATTCGC ACTCGTTTGT GAACCACTAC GCCAATGTGA ACGAGCCATT GCGACCGTCC 
TGGAAGCGCC AAAAGCCGGT CAGGAATTTC TCGAGTCACA CTGATTCGGA ACCGGAAGGT 
TCGGCGAGTT TGAATGGAGG ACAGATTATC ATTAATAACA TGGCGAGGTC AAGGGCGCCC 
CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100142209 ( XM_008199451.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008197673.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X5, mRNA.

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CloneID	OTg12685
Clone ID Related Accession (Same CDS sequence)	XM_008199451.2
Accession Version	XM_008199451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008199451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGGCCCAATT 
CCAGATCCTT TACTCAATTG GGTAACTGAA CTCAGTAACG TATCAGCCGA ACAAAGGTGG 
ATTTTATTAA CAAATTTAAA AGCAGCTGCT GCATATCAGT TCCGAGTTAG CGCCGTTAAT 
AGTGTGGGAG AAGGAAGTCC GTCCGAACCC AGTAACCAAG TCATGTTACC TCAAGAAGCC 
CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT TCAGCCCGAA GTTCCTCCGA AATCATAACA 
CAGTGGCAGC CACCTCTCGA AGAGCACCGT AACGGTCAAA TCCTCGGTTA CATAATCAGA 
TACAAATTAT TCGGTTACAC CAACAGTCCT TGGATCGTTC AAAACATAAC AAACGAAGCG 
CAAAGAAATT ACTTAATTCA AGATTTAATA ACTTGGAAAG ATTACATCGT CCAGATCGCC 
GCCTACAACA ACAAAGGCGT TGGGGTTTTC ACCGAAGGCG CCCGAATTAA GACCAAGGAG 
GGCGTCCCCG AAGCCCCCCC TATAATTCAA ACGGTGGTCC CCCTGAACTC CACCACAATC 
CAAATCACGT GGAAACCGCC AGATCCGCAA AAAATCAACG GAATCAACCA GGGATATAAA 
ATCCAAGCGT GGCAAACCGA CAAAAACGGC GACTTATTTG AAGCCAAAAT GATAACAGTT 
CACCCGAATT TACTCGACCC GAACCAGGAA CAAACGGCGC TTATGACGGG CCTAGAGAAG 
TTCACCGACT ACAATGTTAC GGTCCTGTGC TTCACCGACC CTGGTGATGG CGAAATCAGC 
GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT GTACCTGACG AAGTCAGCAA CTTGCAATTC 
GACGACATTA GCGACCGCGC CGTTAAAGTC ATCTGGCAGC CCCCCAAGCA CATCAACGGC 
ATCTTAACGG GGTACCAAAT CCGGTACCAA ATTCGCGACA AGCCCGACAC TGTCAAAATC 
AAGAACCTCA CAGCCGATAC CCTCAGCCTC AAAGTAACCG ACTTGCAAGC CACCACTCAT 
TACAAATTTG AGGTCACGGC CTGGACTGCA ATGGGGGCAG GCCCCCCTAA AGTGGCGATC 
ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC CATCCCCCCA ACAAACTCGC CTTATCCAAC 
ATTGAGGCCT TCTCTGTAGT TTTGCAATTC ACCCCCGGCT TTGACGGCAA CTCCTCCATC 
ACCAAGTGGA CGGTGGAGGC CCAAACTGCG CGCAACGCCT CCTGGTTTGT CGTTTTCGAA 
ATAAGCGATC CAGACGCAAC TACGATTACT GTTACGGGAC TTGTCCCTTT CACTGTCTAT 
AAACTGCGGT TGATTGCGAA TAATGTCGTG GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG 
GAGTTCCAGA CGATCCAAGC GCCCCCAGCA CACCCCCCGA AGAACGTCAC AGTGCGGGCC 
ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG ATTCCTCTGC AGCAAATCGA ATGGTTCGGG 
AACCCGCGAG GGTACTCGAT TAGTTACGTG GAGGTGCGAA CGGGGGAAAA ACAGACGGCG 
ACAATTGACG ATCATACGGC CAATTCGCAT GTGATTACCG GATTGGAGGA GTTTGCGTTG 
TATGAAGTGT CCATGCAGGC GTGCAATGAC GTGGGGTGTT CCATGGACGG GCCCGTGGCT 
TTGGAGAGGA CCAGGGAGTC AGTACCATCG TTCGGGCCGT TAAACATCGA AGCGAACGCT 
ACCTCTAGCA CAACAATCGT AGTGAAATGG GGTGACGTCC CGAAAGAACA CCAAAACGGC 
CTAATTGAAG GCTACAAAGT TTATTACGCC GCCGAATCAA AATCGCCCGT ACAGTACAAG 
CTAATCGCCA GCAATTCCAC TTTTACAACC ACTTTGACCG AATTGCGGAA ATTCGTCGTT 
TACCGCATCC AGGTCCTGGC GTACACACGG CTCGGCGACG GCGAGCCCAG CAAACCGGCC 
GTCAGCGTGC GAACCTTCGA AGATGTCCCG GGCCCCCCCT CGAATGTGTC ATTCCCGGAC 
GTTTCCGTCA CTTCGGCCAG GATCATCTGG GACGTCCCGG AAGAACCCAA CGGCGAAATC 
CTGGCGTACA AAGTCACATA TCATATCGAC AACGTGAACG TTAATAATAA ATACTCGAAG 
GAATTTCCGC CGTCTGATAG AACGTTCAGG TTTACGCAGT TGGAGCCTGA GAAGTATTAC 
ATGTTTTCTG TGACTGCTCA GACCAGATTA GGTTGGGGCA AGACAGCTCA TGCTTTGGTT 
TTTACAACGA ATAATCGAGA CACACCACAG TCACCATCTA TGCCACAAAT CAGTCGCAGT 
CAGGTACAGA GCAAGCAGAT CACTTTCAGC TGGACACCGG GCAGAGACGG ATTCGCACCG 
TTGAGATATT ATACGGTCCA AAAAATGGAA AATGGGGGCC CTTGGCAGTC CCTTCCCGAA 
CGTGTGGACC CTCAACTCAC CTCATACACC GTCTCTGACC TCAAACCCTT CACTTCATAC 
CGCTTCAGAA TCCGCGCAAC GAACGATATA GGTCCCAGCC CCTTCAGTCC CGAATCCATC 
GAAGTCCGCA CTTATCCAGC AGCCCCCAGC AAAGGCGTCA TGAACTTAAA AGTAGTTCCC 
ATAACAACGA CAAGTGTCGA AGTCTATTGG GACCCAGTTG AGGAGAAGTA CTGGAACGGG 
GACGTCAAAA CAGGGGGCTA TAGAGTAGTC TTTCAACCCG TCTCTGACTA TCCAACTGCA 
CTGCAGGCAA CACCCAAGGA GGAAATTTTC GGAATCAATG CCAAAAAAAT GGTTCTGAGC 
GAATTATTGC AAGACAAGAA TTATGAGATA GTTGTGGTGC CGTTTAATTC GCAAGGGGAG 
GGCCCCGCAA CGCCCCCTGT CACAGTGTAT GTAGGGGAAG CCGTCCCAAC TGGTGAACCA 
AGGGACTTGG AGGGCGAAGC TGTTTCCTCG ACGGAAGTTC GCTTGAAATG GAAGCCGCCA 
CAGCAGCAGC AACAAAACGG CGAATTGTTG GGATACAAGA TATTTTATTT GGTGACAAAC 
TCGCCGCAAG ATTTTGACGA GGGCCACAAG TTCGAGGAGG AAATCGAAGT GATTGCAGCT 
ACGATGACCT CGCACAGCCT TGTCTTTCTC GACAAATACA CCGAATATCG CATACAAATT 
CTGGCGTTTA ACCCAGCAGG TGATGGACCA CGATCGCGCC CCATCACAGT TAAGACCCTT 
CAAGGTCTCC CTGGTCCACC AGCCAATCTA CGCTTTTCTG ACATAACGAT GAATAGCTTG 
CTCGTGTCGT GGGATCCCCC GAAAAAACGC AACGGTGACA TAATCGGATA CCTTGTCACG 
TATGAGACGA CCGAGGAGAA CGAACGTTTT AGTAAACAAG TCAAGCAGAA AGTGACGTCG 
ACGGAACTCT TGCTACAAAC TTTGGAGGAG GAAATCACTT ACACGTTCAC AGTGCGGGCA 
CAAACCATCG ATTATGGGCC CTCTATTTCG GATAACGTCA CCACAGGGCC ACAGGACGGC 
TCCCCAACGA CGCCAAAAGA ACTCACTTTG ATTAAAACAC CGTCAAGTGT GGAGCTGCAC 
TGGGTCAATG GCTTGACCGG AAAGGGGCCA ATTCTGGGCT ACTATATCGA ATCGAGGAAA 
AAAGATGACC AGAGGTGGCA AACAGTCACG AAATCGACAA ACGGGCCTTT GCAAGAATTT 
ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA ACCTCTTACT ATTTCAGAGT TTTTCCTTAC 
AATAAATTCG GTATTAGTTG TCCCGCGTAC ACCAAAGACC CGGTCGTAAC ACCGTCAAAA 
CTGTACTTGG AATATAGCTA CTTACAGTAT GAACCCTTCT ACCGACAGAC CTGGTTCATG 
GTCGCCCTGG CAGCGACCTC CATCATCATT ATCATAATGA TAATTGCGAT TTTGTGCGTT 
AAGAGCAAAA GTTACAAATA TAAACAAGGT GCCCAAAATA GTATGGGCGA ACTCATGGCA 
ATGTCACGCG ACGACATGAC CGCTGACTAT TACCGAACTC GCAACGGCAC AAGTAACCTA 
AGCTCGACTC TTTCAAAACG TAGCACTTTA TCCCGCAAGC CGGTCCATCA ACAACCCCCT 
CCAATGTTGG GGAAATCCCC TCCGAGACCA TCGCCGGCTT CAGTGCCCTA CAACTCAGAC 
GAGGAAAGTC TCAAGGGCTA CGATGAGAAT CCCGACGATA GCAGCGTGAC CGAGAAGCCG 
TCAGAAATGA GCTCTTCTGA ATCCCAGGGT TCGGAAAGCG AAAACGACAG TGTCCTCTCG 
AATTCGCACT CGTTTGTGAA CCACTACGCC AATGTGAACG AGCCATTGCG ACCGTCCTGG 
AAGCGCCAAA AGCCGGTCAG GAATTTCTCG AGTCACACTG ATTCGGAACC GGAAGGTTCG 
GCGAGTTTGA ATGGAGGACA GATTATCATT AATAACATGG CGAGGTCAAG GGCGCCCCTA 
CCCGGATTTT CGTCGTTTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008197673.1
CDS	385..6888
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKTAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELGPIPDPLLNWVTELSNVSAEQRWILLTNLKAAAAYQFRVSAVNSVGEGSPSEPSNQVMLPQEAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_008199451.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008199451.2

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA CTGCGGGTCC CGTGATGGAA TTAGGGCCTC AAAATGCCAC AGTTCTCGAT 
GGTAAAGACG CAACTTTAGC TTGCCGAGCT GTAGGAGCCC CAACACCCAA TATCACCTGG 
TTTTACAAAG GGGATCTTAT TGAGACCTCA AGCCGCGTCC AAATTCTCGA CACTGGAGAC 
TTGCTAATAG CTGCTGTTAA GGAATCAGAT GTTGGAATGT ACACTTGTTT AAGAGCCAAC 
GAAGCCGGCG AAGTACGAGG CTCTGCACAT TTGGGAGTCT TGGTTCGCAC CCAAATCATC 
CAACCTCCGG TGGACACGCG GGTGCTCCTC GGGCATACGG CCACTCTTCA GTGTAAAATC 
TCCTCCGATC CTGCTGTCCC TTTCGACTTG CGGTGGTACA AAAATAAACA AGCTTTAAAT 
CCGAAAGCGA GTTCAAGGAT AAGTATTTTA AAGGATGGCA CTTTGGAAAT ACAAGCAGTT 
CGAGCTGCGG ATGTCGGTCT CTACACATGC TCGATTAAAT CACCTGGTGG TAACGAAACT 
AGATCGGCTA AATTGAGCGT TATCGAACTA CCATACGCCC CTACTAATGT TAAAGCCGAA 
AGACTGGATA CGGCCTCGCA AAGGGCGGTT AATGTCAGTT GGATACCTGG ATTCGATGGA 
AATAGTCCGA TAAAACAATT TATTATACAA AAAAGAGAGT TGCCTGAACT TGGCCCAATT 
CCAGATCCTT TACTCAATTG GGTAACTGAA CTCAGTAACG TATCAGCCGA ACAAAGGTGG 
ATTTTATTAA CAAATTTAAA AGCAGCTGCT GCATATCAGT TCCGAGTTAG CGCCGTTAAT 
AGTGTGGGAG AAGGAAGTCC GTCCGAACCC AGTAACCAAG TCATGTTACC TCAAGAAGCC 
CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT TCAGCCCGAA GTTCCTCCGA AATCATAACA 
CAGTGGCAGC CACCTCTCGA AGAGCACCGT AACGGTCAAA TCCTCGGTTA CATAATCAGA 
TACAAATTAT TCGGTTACAC CAACAGTCCT TGGATCGTTC AAAACATAAC AAACGAAGCG 
CAAAGAAATT ACTTAATTCA AGATTTAATA ACTTGGAAAG ATTACATCGT CCAGATCGCC 
GCCTACAACA ACAAAGGCGT TGGGGTTTTC ACCGAAGGCG CCCGAATTAA GACCAAGGAG 
GGCGTCCCCG AAGCCCCCCC TATAATTCAA ACGGTGGTCC CCCTGAACTC CACCACAATC 
CAAATCACGT GGAAACCGCC AGATCCGCAA AAAATCAACG GAATCAACCA GGGATATAAA 
ATCCAAGCGT GGCAAACCGA CAAAAACGGC GACTTATTTG AAGCCAAAAT GATAACAGTT 
CACCCGAATT TACTCGACCC GAACCAGGAA CAAACGGCGC TTATGACGGG CCTAGAGAAG 
TTCACCGACT ACAATGTTAC GGTCCTGTGC TTCACCGACC CTGGTGATGG CGAAATCAGC 
GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT GTACCTGACG AAGTCAGCAA CTTGCAATTC 
GACGACATTA GCGACCGCGC CGTTAAAGTC ATCTGGCAGC CCCCCAAGCA CATCAACGGC 
ATCTTAACGG GGTACCAAAT CCGGTACCAA ATTCGCGACA AGCCCGACAC TGTCAAAATC 
AAGAACCTCA CAGCCGATAC CCTCAGCCTC AAAGTAACCG ACTTGCAAGC CACCACTCAT 
TACAAATTTG AGGTCACGGC CTGGACTGCA ATGGGGGCAG GCCCCCCTAA AGTGGCGATC 
ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC CATCCCCCCA ACAAACTCGC CTTATCCAAC 
ATTGAGGCCT TCTCTGTAGT TTTGCAATTC ACCCCCGGCT TTGACGGCAA CTCCTCCATC 
ACCAAGTGGA CGGTGGAGGC CCAAACTGCG CGCAACGCCT CCTGGTTTGT CGTTTTCGAA 
ATAAGCGATC CAGACGCAAC TACGATTACT GTTACGGGAC TTGTCCCTTT CACTGTCTAT 
AAACTGCGGT TGATTGCGAA TAATGTCGTG GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG 
GAGTTCCAGA CGATCCAAGC GCCCCCAGCA CACCCCCCGA AGAACGTCAC AGTGCGGGCC 
ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG ATTCCTCTGC AGCAAATCGA ATGGTTCGGG 
AACCCGCGAG GGTACTCGAT TAGTTACGTG GAGGTGCGAA CGGGGGAAAA ACAGACGGCG 
ACAATTGACG ATCATACGGC CAATTCGCAT GTGATTACCG GATTGGAGGA GTTTGCGTTG 
TATGAAGTGT CCATGCAGGC GTGCAATGAC GTGGGGTGTT CCATGGACGG GCCCGTGGCT 
TTGGAGAGGA CCAGGGAGTC AGTACCATCG TTCGGGCCGT TAAACATCGA AGCGAACGCT 
ACCTCTAGCA CAACAATCGT AGTGAAATGG GGTGACGTCC CGAAAGAACA CCAAAACGGC 
CTAATTGAAG GCTACAAAGT TTATTACGCC GCCGAATCAA AATCGCCCGT ACAGTACAAG 
CTAATCGCCA GCAATTCCAC TTTTACAACC ACTTTGACCG AATTGCGGAA ATTCGTCGTT 
TACCGCATCC AGGTCCTGGC GTACACACGG CTCGGCGACG GCGAGCCCAG CAAACCGGCC 
GTCAGCGTGC GAACCTTCGA AGATGTCCCG GGCCCCCCCT CGAATGTGTC ATTCCCGGAC 
GTTTCCGTCA CTTCGGCCAG GATCATCTGG GACGTCCCGG AAGAACCCAA CGGCGAAATC 
CTGGCGTACA AAGTCACATA TCATATCGAC AACGTGAACG TTAATAATAA ATACTCGAAG 
GAATTTCCGC CGTCTGATAG AACGTTCAGG TTTACGCAGT TGGAGCCTGA GAAGTATTAC 
ATGTTTTCTG TGACTGCTCA GACCAGATTA GGTTGGGGCA AGACAGCTCA TGCTTTGGTT 
TTTACAACGA ATAATCGAGA CACACCACAG TCACCATCTA TGCCACAAAT CAGTCGCAGT 
CAGGTACAGA GCAAGCAGAT CACTTTCAGC TGGACACCGG GCAGAGACGG ATTCGCACCG 
TTGAGATATT ATACGGTCCA AAAAATGGAA AATGGGGGCC CTTGGCAGTC CCTTCCCGAA 
CGTGTGGACC CTCAACTCAC CTCATACACC GTCTCTGACC TCAAACCCTT CACTTCATAC 
CGCTTCAGAA TCCGCGCAAC GAACGATATA GGTCCCAGCC CCTTCAGTCC CGAATCCATC 
GAAGTCCGCA CTTATCCAGC AGCCCCCAGC AAAGGCGTCA TGAACTTAAA AGTAGTTCCC 
ATAACAACGA CAAGTGTCGA AGTCTATTGG GACCCAGTTG AGGAGAAGTA CTGGAACGGG 
GACGTCAAAA CAGGGGGCTA TAGAGTAGTC TTTCAACCCG TCTCTGACTA TCCAACTGCA 
CTGCAGGCAA CACCCAAGGA GGAAATTTTC GGAATCAATG CCAAAAAAAT GGTTCTGAGC 
GAATTATTGC AAGACAAGAA TTATGAGATA GTTGTGGTGC CGTTTAATTC GCAAGGGGAG 
GGCCCCGCAA CGCCCCCTGT CACAGTGTAT GTAGGGGAAG CCGTCCCAAC TGGTGAACCA 
AGGGACTTGG AGGGCGAAGC TGTTTCCTCG ACGGAAGTTC GCTTGAAATG GAAGCCGCCA 
CAGCAGCAGC AACAAAACGG CGAATTGTTG GGATACAAGA TATTTTATTT GGTGACAAAC 
TCGCCGCAAG ATTTTGACGA GGGCCACAAG TTCGAGGAGG AAATCGAAGT GATTGCAGCT 
ACGATGACCT CGCACAGCCT TGTCTTTCTC GACAAATACA CCGAATATCG CATACAAATT 
CTGGCGTTTA ACCCAGCAGG TGATGGACCA CGATCGCGCC CCATCACAGT TAAGACCCTT 
CAAGGTCTCC CTGGTCCACC AGCCAATCTA CGCTTTTCTG ACATAACGAT GAATAGCTTG 
CTCGTGTCGT GGGATCCCCC GAAAAAACGC AACGGTGACA TAATCGGATA CCTTGTCACG 
TATGAGACGA CCGAGGAGAA CGAACGTTTT AGTAAACAAG TCAAGCAGAA AGTGACGTCG 
ACGGAACTCT TGCTACAAAC TTTGGAGGAG GAAATCACTT ACACGTTCAC AGTGCGGGCA 
CAAACCATCG ATTATGGGCC CTCTATTTCG GATAACGTCA CCACAGGGCC ACAGGACGGC 
TCCCCAACGA CGCCAAAAGA ACTCACTTTG ATTAAAACAC CGTCAAGTGT GGAGCTGCAC 
TGGGTCAATG GCTTGACCGG AAAGGGGCCA ATTCTGGGCT ACTATATCGA ATCGAGGAAA 
AAAGATGACC AGAGGTGGCA AACAGTCACG AAATCGACAA ACGGGCCTTT GCAAGAATTT 
ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA ACCTCTTACT ATTTCAGAGT TTTTCCTTAC 
AATAAATTCG GTATTAGTTG TCCCGCGTAC ACCAAAGACC CGGTCGTAAC ACCGTCAAAA 
CTGTACTTGG AATATAGCTA CTTACAGTAT GAACCCTTCT ACCGACAGAC CTGGTTCATG 
GTCGCCCTGG CAGCGACCTC CATCATCATT ATCATAATGA TAATTGCGAT TTTGTGCGTT 
AAGAGCAAAA GTTACAAATA TAAACAAGGT GCCCAAAATA GTATGGGCGA ACTCATGGCA 
ATGTCACGCG ACGACATGAC CGCTGACTAT TACCGAACTC GCAACGGCAC AAGTAACCTA 
AGCTCGACTC TTTCAAAACG TAGCACTTTA TCCCGCAAGC CGGTCCATCA ACAACCCCCT 
CCAATGTTGG GGAAATCCCC TCCGAGACCA TCGCCGGCTT CAGTGCCCTA CAACTCAGAC 
GAGGAAAGTC TCAAGGGCTA CGATGAGAAT CCCGACGATA GCAGCGTGAC CGAGAAGCCG 
TCAGAAATGA GCTCTTCTGA ATCCCAGGGT TCGGAAAGCG AAAACGACAG TGTCCTCTCG 
AATTCGCACT CGTTTGTGAA CCACTACGCC AATGTGAACG AGCCATTGCG ACCGTCCTGG 
AAGCGCCAAA AGCCGGTCAG GAATTTCTCG AGTCACACTG ATTCGGAACC GGAAGGTTCG 
GCGAGTTTGA ATGGAGGACA GATTATCATT AATAACATGG CGAGGTCAAG GGCGCCCCTA 
CCCGGATTTT CGTCGTTTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100142209 ( XM_015983207.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015838693.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X1, mRNA.

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CloneID	OTg21404
Clone ID Related Accession (Same CDS sequence)	XM_015983207.1
Accession Version	XM_015983207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA GTAAGTATTG GCATAAAACT CAAACCCGAA ATAAAATTTT TCACCACTAT 
CATGCAGAGC GGAGAAAAGT GAGAAGTTTA GCGGCTAGAT ATGCCAGATT GAGAAGTTCC 
GGCGAACGCC ATAGCAAAAA GAATTTTGTT TTTGTGACAC AATCTGCGGG TCCCGTGATG 
GAATTAGGGC CTCAAAATGC CACAGTTCTC GATGGTAAAG ACGCAACTTT AGCTTGCCGA 
GCTGTAGGAG CCCCAACACC CAATATCACC TGGTTTTACA AAGGGGATCT TATTGAGACC 
TCAAGCCGCG TCCAAATTCT CGACACTGGA GACTTGCTAA TAGCTGCTGT TAAGGAATCA 
GATGTTGGAA TGTACACTTG TTTAAGAGCC AACGAAGCCG GCGAAGTACG AGGCTCTGCA 
CATTTGGGAG TCTTGGTTCG CACCCAAATC ATCCAACCTC CGGTGGACAC GCGGGTGCTC 
CTCGGGCATA CGGCCACTCT TCAGTGTAAA ATCTCCTCCG ATCCTGCTGT CCCTTTCGAC 
TTGCGGTGGT ACAAAAATAA ACAAGCTTTA AATCCGAAAG CGAGTTCAAG GATAAGTATT 
TTAAAGGATG GCACTTTGGA AATACAAGCA GTTCGAGCTG CGGATGTCGG TCTCTACACA 
TGCTCGATTA AATCACCTGG TGGTAACGAA ACTAGATCGG CTAAATTGAG CGTTATCGAA 
CTACCATACG CCCCTACTAA TGTTAAAGCC GAAAGACTGG ATACGGCCTC GCAAAGGGCG 
GTTAATGTCA GTTGGATACC TGGATTCGAT GGAAATAGTC CGATAAAACA ATTTATTATA 
CAAAAAAGAG AGTTGCCTGA ACTTGGCCCA ATTCCAGATC CTTTACTCAA TTGGGTAACT 
GAACTCAGTA ACGTATCAGC CGAACAAAGG TGGATTTTAT TAACAAATTT AAAAGCAGCT 
GCTGCATATC AGTTCCGAGT TAGCGCCGTT AATAGTGTGG GAGAAGGAAG TCCGTCCGAA 
CCCAGTAACC AAGTCATGTT ACCTCAAGAA GCCCCTTCTG GACCTCCTGT TGGTTTCGTG 
GGTTCAGCCC GAAGTTCCTC CGAAATCATA ACACAGTGGC AGCCACCTCT CGAAGAGCAC 
CGTAACGGTC AAATCCTCGG TTACATAATC AGATACAAAT TATTCGGTTA CACCAACAGT 
CCTTGGATCG TTCAAAACAT AACAAACGAA GCGCAAAGAA ATTACTTAAT TCAAGATTTA 
ATAACTTGGA AAGATTACAT CGTCCAGATC GCCGCCTACA ACAACAAAGG CGTTGGGGTT 
TTCACCGAAG GCGCCCGAAT TAAGACCAAG GAGGGCGTCC CCGAAGCCCC CCCTATAATT 
CAAACGGTGG TCCCCCTGAA CTCCACCACA ATCCAAATCA CGTGGAAACC GCCAGATCCG 
CAAAAAATCA ACGGAATCAA CCAGGGATAT AAAATCCAAG CGTGGCAAAC CGACAAAAAC 
GGCGACTTAT TTGAAGCCAA AATGATAACA GTTCACCCGA ATTTACTCGA CCCGAACCAG 
GAACAAACGG CGCTTATGAC GGGCCTAGAG AAGTTCACCG ACTACAATGT TACGGTCCTG 
TGCTTCACCG ACCCTGGTGA TGGCGAAATC AGCGAGTTTG TCCCAGTGAA AACAAAAGAA 
GATGTACCTG ACGAAGTCAG CAACTTGCAA TTCGACGACA TTAGCGACCG CGCCGTTAAA 
GTCATCTGGC AGCCCCCCAA GCACATCAAC GGCATCTTAA CGGGGTACCA AATCCGGTAC 
CAAATTCGCG ACAAGCCCGA CACTGTCAAA ATCAAGAACC TCACAGCCGA TACCCTCAGC 
CTCAAAGTAA CCGACTTGCA AGCCACCACT CATTACAAAT TTGAGGTCAC GGCCTGGACT 
GCAATGGGGG CAGGCCCCCC TAAAGTGGCG ATCATCCAGT CGGGGGTGGA GCCCGTCTTG 
CCCCATCCCC CCAACAAACT CGCCTTATCC AACATTGAGG CCTTCTCTGT AGTTTTGCAA 
TTCACCCCCG GCTTTGACGG CAACTCCTCC ATCACCAAGT GGACGGTGGA GGCCCAAACT 
GCGCGCAACG CCTCCTGGTT TGTCGTTTTC GAAATAAGCG ATCCAGACGC AACTACGATT 
ACTGTTACGG GACTTGTCCC TTTCACTGTC TATAAACTGC GGTTGATTGC GAATAATGTC 
GTGGGGGCTT CCGAGCCCTC CGAAGCCTCC AAGGAGTTCC AGACGATCCA AGCGCCCCCA 
GCACACCCCC CGAAGAACGT CACAGTGCGG GCCATGAGCG CGACTGAAGT GAGGGTTCGC 
TGGATTCCTC TGCAGCAAAT CGAATGGTTC GGGAACCCGC GAGGGTACTC GATTAGTTAC 
GTGGAGGTGC GAACGGGGGA AAAACAGACG GCGACAATTG ACGATCATAC GGCCAATTCG 
CATGTGATTA CCGGATTGGA GGAGTTTGCG TTGTATGAAG TGTCCATGCA GGCGTGCAAT 
GACGTGGGGT GTTCCATGGA CGGGCCCGTG GCTTTGGAGA GGACCAGGGA GTCAGTACCA 
TCGTTCGGGC CGTTAAACAT CGAAGCGAAC GCTACCTCTA GCACAACAAT CGTAGTGAAA 
TGGGGTGACG TCCCGAAAGA ACACCAAAAC GGCCTAATTG AAGGCTACAA AGTTTATTAC 
GCCGCCGAAT CAAAATCGCC CGTACAGTAC AAGCTAATCG CCAGCAATTC CACTTTTACA 
ACCACTTTGA CCGAATTGCG GAAATTCGTC GTTTACCGCA TCCAGGTCCT GGCGTACACA 
CGGCTCGGCG ACGGCGAGCC CAGCAAACCG GCCGTCAGCG TGCGAACCTT CGAAGATGTC 
CCGGGCCCCC CCTCGAATGT GTCATTCCCG GACGTTTCCG TCACTTCGGC CAGGATCATC 
TGGGACGTCC CGGAAGAACC CAACGGCGAA ATCCTGGCGT ACAAAGTCAC ATATCATATC 
GACAACGTGA ACGTTAATAA TAAATACTCG AAGGAATTTC CGCCGTCTGA TAGAACGTTC 
AGGTTTACGC AGTTGGAGCC TGAGAAGTAT TACATGTTTT CTGTGACTGC TCAGACCAGA 
TTAGGTTGGG GCAAGACAGC TCATGCTTTG GTTTTTACAA CGAATAATCG AGACACACCA 
CAGTCACCAT CTATGCCACA AATCAGTCGC AGTCAGGTAC AGAGCAAGCA GATCACTTTC 
AGCTGGACAC CGGGCAGAGA CGGATTCGCA CCGTTGAGAT ATTATACGGT CCAAAAAATG 
GAAAATGGGG GCCCTTGGCA GTCCCTTCCC GAACGTGTGG ACCCTCAACT CACCTCATAC 
ACCGTCTCTG ACCTCAAACC CTTCACTTCA TACCGCTTCA GAATCCGCGC AACGAACGAT 
ATAGGTCCCA GCCCCTTCAG TCCCGAATCC ATCGAAGTCC GCACTTATCC AGCAGCCCCC 
AGCAAAGGCG TCATGAACTT AAAAGTAGTT CCCATAACAA CGACAAGTGT CGAAGTCTAT 
TGGGACCCAG TTGAGGAGAA GTACTGGAAC GGGGACGTCA AAACAGGGGG CTATAGAGTA 
GTCTTTCAAC CCGTCTCTGA CTATCCAACT GCACTGCAGG CAACACCCAA GGAGGAAATT 
TTCGGAATCA ATGCCAAAAA AATGGTTCTG AGCGAATTAT TGCAAGACAA GAATTATGAG 
ATAGTTGTGG TGCCGTTTAA TTCGCAAGGG GAGGGCCCCG CAACGCCCCC TGTCACAGTG 
TATGTAGGGG AAGCCGTCCC AACTGGTGAA CCAAGGGACT TGGAGGGCGA AGCTGTTTCC 
TCGACGGAAG TTCGCTTGAA ATGGAAGCCG CCACAGCAGC AGCAACAAAA CGGCGAATTG 
TTGGGATACA AGATATTTTA TTTGGTGACA AACTCGCCGC AAGATTTTGA CGAGGGCCAC 
AAGTTCGAGG AGGAAATCGA AGTGATTGCA GCTACGATGA CCTCGCACAG CCTTGTCTTT 
CTCGACAAAT ACACCGAATA TCGCATACAA ATTCTGGCGT TTAACCCAGC AGGTGATGGA 
CCACGATCGC GCCCCATCAC AGTTAAGACC CTTCAAGGTC TCCCTGGTCC ACCAGCCAAT 
CTACGCTTTT CTGACATAAC GATGAATAGC TTGCTCGTGT CGTGGGATCC CCCGAAAAAA 
CGCAACGGTG ACATAATCGG ATACCTTGTC ACGTATGAGA CGACCGAGGA GAACGAACGT 
TTTAGTAAAC AAGTCAAGCA GAAAGTGACG TCGACGGAAC TCTTGCTACA AACTTTGGAG 
GAGGAAATCA CTTACACGTT CACAGTGCGG GCACAAACCA TCGATTATGG GCCCTCTATT 
TCGGATAACG TCACCACAGG GCCACAGGAC GGCTCCCCAA CGACGCCAAA AGAACTCACT 
TTGATTAAAA CACCGTCAAG TGTGGAGCTG CACTGGGTCA ATGGCTTGAC CGGAAAGGGG 
CCAATTCTGG GCTACTATAT CGAATCGAGG AAAAAAGATG ACCAGAGGTG GCAAACAGTC 
ACGAAATCGA CAAACGGGCC TTTGCAAGAA TTTACAGTTT CGTACCAAAG TCTCCTCCCC 
TCAACCTCTT ACTATTTCAG AGTTTTTCCT TACAATAAAT TCGGTATTAG TTGTCCCGCG 
TACACCAAAG ACCCGGTCGT AACACCGTCA AAACTGTACT TGGAATATAG CTACTTACAG 
TATGAACCCT TCTACCGACA GACCTGGTTC ATGGTCGCCC TGGCAGCGAC CTCCATCATC 
ATTATCATAA TGATAATTGC GATTTTGTGC GTTAAGAGCA AAAGTTACAA ATATAAACAA 
GGTGCCCAAA ATAGTATGGG CGAACTCATG GCAATGTCAC GCGACGACAT GACCGCTGAC 
TATTACCGAA CTCGCAACGG CACAAGTAAC CTAAGCTCGA CTCTTTCAAA ACGTAGCACT 
TTATCCCGCA AGCCGGTCCA TCAACAACCC CCTCCAATGT TGGGGAAATC CCCTCCGAGA 
CCATCGCCGG CTTCAGTGCC CTACAACTCA GACGAGGAAA GTCTCAAGGG CTACGATGAG 
AATCCCGACG ATAGCAGCGT GACCGAGAAG CCGTCAGAAA TGAGCTCTTC TGAATCCCAG 
GGTTCGGAAA GCGAAAACGA CAGTGTCCTC TCGAATTCGC ACTCGTTTGT GAACCACTAC 
GCCAATGTGA ACGAGCCATT GCGACCGTCC TGGAAGCGCC AAAAGCCGGT CAGGAATTTC 
TCGAGTCACA CTGATTCGGA ACCGGAAGGT TCGGCGAGTT TGAATGGAGG ACAGATTATC 
ATTAATAACA TGGCGAGGTC AAGGGCGCCC CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015838693.1
CDS	385..7041
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKSKYWHKTQTRNKIFHHYHAERRKVRSLAARYARLRSSGERHSKKNFVFVTQSAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELGPIPDPLLNWVTELSNVSAEQRWILLTNLKAAAAYQFRVSAVNSVGEGSPSEPSNQVMLPQEAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_015983207.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015983207.1

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA GTAAGTATTG GCATAAAACT CAAACCCGAA ATAAAATTTT TCACCACTAT 
CATGCAGAGC GGAGAAAAGT GAGAAGTTTA GCGGCTAGAT ATGCCAGATT GAGAAGTTCC 
GGCGAACGCC ATAGCAAAAA GAATTTTGTT TTTGTGACAC AATCTGCGGG TCCCGTGATG 
GAATTAGGGC CTCAAAATGC CACAGTTCTC GATGGTAAAG ACGCAACTTT AGCTTGCCGA 
GCTGTAGGAG CCCCAACACC CAATATCACC TGGTTTTACA AAGGGGATCT TATTGAGACC 
TCAAGCCGCG TCCAAATTCT CGACACTGGA GACTTGCTAA TAGCTGCTGT TAAGGAATCA 
GATGTTGGAA TGTACACTTG TTTAAGAGCC AACGAAGCCG GCGAAGTACG AGGCTCTGCA 
CATTTGGGAG TCTTGGTTCG CACCCAAATC ATCCAACCTC CGGTGGACAC GCGGGTGCTC 
CTCGGGCATA CGGCCACTCT TCAGTGTAAA ATCTCCTCCG ATCCTGCTGT CCCTTTCGAC 
TTGCGGTGGT ACAAAAATAA ACAAGCTTTA AATCCGAAAG CGAGTTCAAG GATAAGTATT 
TTAAAGGATG GCACTTTGGA AATACAAGCA GTTCGAGCTG CGGATGTCGG TCTCTACACA 
TGCTCGATTA AATCACCTGG TGGTAACGAA ACTAGATCGG CTAAATTGAG CGTTATCGAA 
CTACCATACG CCCCTACTAA TGTTAAAGCC GAAAGACTGG ATACGGCCTC GCAAAGGGCG 
GTTAATGTCA GTTGGATACC TGGATTCGAT GGAAATAGTC CGATAAAACA ATTTATTATA 
CAAAAAAGAG AGTTGCCTGA ACTTGGCCCA ATTCCAGATC CTTTACTCAA TTGGGTAACT 
GAACTCAGTA ACGTATCAGC CGAACAAAGG TGGATTTTAT TAACAAATTT AAAAGCAGCT 
GCTGCATATC AGTTCCGAGT TAGCGCCGTT AATAGTGTGG GAGAAGGAAG TCCGTCCGAA 
CCCAGTAACC AAGTCATGTT ACCTCAAGAA GCCCCTTCTG GACCTCCTGT TGGTTTCGTG 
GGTTCAGCCC GAAGTTCCTC CGAAATCATA ACACAGTGGC AGCCACCTCT CGAAGAGCAC 
CGTAACGGTC AAATCCTCGG TTACATAATC AGATACAAAT TATTCGGTTA CACCAACAGT 
CCTTGGATCG TTCAAAACAT AACAAACGAA GCGCAAAGAA ATTACTTAAT TCAAGATTTA 
ATAACTTGGA AAGATTACAT CGTCCAGATC GCCGCCTACA ACAACAAAGG CGTTGGGGTT 
TTCACCGAAG GCGCCCGAAT TAAGACCAAG GAGGGCGTCC CCGAAGCCCC CCCTATAATT 
CAAACGGTGG TCCCCCTGAA CTCCACCACA ATCCAAATCA CGTGGAAACC GCCAGATCCG 
CAAAAAATCA ACGGAATCAA CCAGGGATAT AAAATCCAAG CGTGGCAAAC CGACAAAAAC 
GGCGACTTAT TTGAAGCCAA AATGATAACA GTTCACCCGA ATTTACTCGA CCCGAACCAG 
GAACAAACGG CGCTTATGAC GGGCCTAGAG AAGTTCACCG ACTACAATGT TACGGTCCTG 
TGCTTCACCG ACCCTGGTGA TGGCGAAATC AGCGAGTTTG TCCCAGTGAA AACAAAAGAA 
GATGTACCTG ACGAAGTCAG CAACTTGCAA TTCGACGACA TTAGCGACCG CGCCGTTAAA 
GTCATCTGGC AGCCCCCCAA GCACATCAAC GGCATCTTAA CGGGGTACCA AATCCGGTAC 
CAAATTCGCG ACAAGCCCGA CACTGTCAAA ATCAAGAACC TCACAGCCGA TACCCTCAGC 
CTCAAAGTAA CCGACTTGCA AGCCACCACT CATTACAAAT TTGAGGTCAC GGCCTGGACT 
GCAATGGGGG CAGGCCCCCC TAAAGTGGCG ATCATCCAGT CGGGGGTGGA GCCCGTCTTG 
CCCCATCCCC CCAACAAACT CGCCTTATCC AACATTGAGG CCTTCTCTGT AGTTTTGCAA 
TTCACCCCCG GCTTTGACGG CAACTCCTCC ATCACCAAGT GGACGGTGGA GGCCCAAACT 
GCGCGCAACG CCTCCTGGTT TGTCGTTTTC GAAATAAGCG ATCCAGACGC AACTACGATT 
ACTGTTACGG GACTTGTCCC TTTCACTGTC TATAAACTGC GGTTGATTGC GAATAATGTC 
GTGGGGGCTT CCGAGCCCTC CGAAGCCTCC AAGGAGTTCC AGACGATCCA AGCGCCCCCA 
GCACACCCCC CGAAGAACGT CACAGTGCGG GCCATGAGCG CGACTGAAGT GAGGGTTCGC 
TGGATTCCTC TGCAGCAAAT CGAATGGTTC GGGAACCCGC GAGGGTACTC GATTAGTTAC 
GTGGAGGTGC GAACGGGGGA AAAACAGACG GCGACAATTG ACGATCATAC GGCCAATTCG 
CATGTGATTA CCGGATTGGA GGAGTTTGCG TTGTATGAAG TGTCCATGCA GGCGTGCAAT 
GACGTGGGGT GTTCCATGGA CGGGCCCGTG GCTTTGGAGA GGACCAGGGA GTCAGTACCA 
TCGTTCGGGC CGTTAAACAT CGAAGCGAAC GCTACCTCTA GCACAACAAT CGTAGTGAAA 
TGGGGTGACG TCCCGAAAGA ACACCAAAAC GGCCTAATTG AAGGCTACAA AGTTTATTAC 
GCCGCCGAAT CAAAATCGCC CGTACAGTAC AAGCTAATCG CCAGCAATTC CACTTTTACA 
ACCACTTTGA CCGAATTGCG GAAATTCGTC GTTTACCGCA TCCAGGTCCT GGCGTACACA 
CGGCTCGGCG ACGGCGAGCC CAGCAAACCG GCCGTCAGCG TGCGAACCTT CGAAGATGTC 
CCGGGCCCCC CCTCGAATGT GTCATTCCCG GACGTTTCCG TCACTTCGGC CAGGATCATC 
TGGGACGTCC CGGAAGAACC CAACGGCGAA ATCCTGGCGT ACAAAGTCAC ATATCATATC 
GACAACGTGA ACGTTAATAA TAAATACTCG AAGGAATTTC CGCCGTCTGA TAGAACGTTC 
AGGTTTACGC AGTTGGAGCC TGAGAAGTAT TACATGTTTT CTGTGACTGC TCAGACCAGA 
TTAGGTTGGG GCAAGACAGC TCATGCTTTG GTTTTTACAA CGAATAATCG AGACACACCA 
CAGTCACCAT CTATGCCACA AATCAGTCGC AGTCAGGTAC AGAGCAAGCA GATCACTTTC 
AGCTGGACAC CGGGCAGAGA CGGATTCGCA CCGTTGAGAT ATTATACGGT CCAAAAAATG 
GAAAATGGGG GCCCTTGGCA GTCCCTTCCC GAACGTGTGG ACCCTCAACT CACCTCATAC 
ACCGTCTCTG ACCTCAAACC CTTCACTTCA TACCGCTTCA GAATCCGCGC AACGAACGAT 
ATAGGTCCCA GCCCCTTCAG TCCCGAATCC ATCGAAGTCC GCACTTATCC AGCAGCCCCC 
AGCAAAGGCG TCATGAACTT AAAAGTAGTT CCCATAACAA CGACAAGTGT CGAAGTCTAT 
TGGGACCCAG TTGAGGAGAA GTACTGGAAC GGGGACGTCA AAACAGGGGG CTATAGAGTA 
GTCTTTCAAC CCGTCTCTGA CTATCCAACT GCACTGCAGG CAACACCCAA GGAGGAAATT 
TTCGGAATCA ATGCCAAAAA AATGGTTCTG AGCGAATTAT TGCAAGACAA GAATTATGAG 
ATAGTTGTGG TGCCGTTTAA TTCGCAAGGG GAGGGCCCCG CAACGCCCCC TGTCACAGTG 
TATGTAGGGG AAGCCGTCCC AACTGGTGAA CCAAGGGACT TGGAGGGCGA AGCTGTTTCC 
TCGACGGAAG TTCGCTTGAA ATGGAAGCCG CCACAGCAGC AGCAACAAAA CGGCGAATTG 
TTGGGATACA AGATATTTTA TTTGGTGACA AACTCGCCGC AAGATTTTGA CGAGGGCCAC 
AAGTTCGAGG AGGAAATCGA AGTGATTGCA GCTACGATGA CCTCGCACAG CCTTGTCTTT 
CTCGACAAAT ACACCGAATA TCGCATACAA ATTCTGGCGT TTAACCCAGC AGGTGATGGA 
CCACGATCGC GCCCCATCAC AGTTAAGACC CTTCAAGGTC TCCCTGGTCC ACCAGCCAAT 
CTACGCTTTT CTGACATAAC GATGAATAGC TTGCTCGTGT CGTGGGATCC CCCGAAAAAA 
CGCAACGGTG ACATAATCGG ATACCTTGTC ACGTATGAGA CGACCGAGGA GAACGAACGT 
TTTAGTAAAC AAGTCAAGCA GAAAGTGACG TCGACGGAAC TCTTGCTACA AACTTTGGAG 
GAGGAAATCA CTTACACGTT CACAGTGCGG GCACAAACCA TCGATTATGG GCCCTCTATT 
TCGGATAACG TCACCACAGG GCCACAGGAC GGCTCCCCAA CGACGCCAAA AGAACTCACT 
TTGATTAAAA CACCGTCAAG TGTGGAGCTG CACTGGGTCA ATGGCTTGAC CGGAAAGGGG 
CCAATTCTGG GCTACTATAT CGAATCGAGG AAAAAAGATG ACCAGAGGTG GCAAACAGTC 
ACGAAATCGA CAAACGGGCC TTTGCAAGAA TTTACAGTTT CGTACCAAAG TCTCCTCCCC 
TCAACCTCTT ACTATTTCAG AGTTTTTCCT TACAATAAAT TCGGTATTAG TTGTCCCGCG 
TACACCAAAG ACCCGGTCGT AACACCGTCA AAACTGTACT TGGAATATAG CTACTTACAG 
TATGAACCCT TCTACCGACA GACCTGGTTC ATGGTCGCCC TGGCAGCGAC CTCCATCATC 
ATTATCATAA TGATAATTGC GATTTTGTGC GTTAAGAGCA AAAGTTACAA ATATAAACAA 
GGTGCCCAAA ATAGTATGGG CGAACTCATG GCAATGTCAC GCGACGACAT GACCGCTGAC 
TATTACCGAA CTCGCAACGG CACAAGTAAC CTAAGCTCGA CTCTTTCAAA ACGTAGCACT 
TTATCCCGCA AGCCGGTCCA TCAACAACCC CCTCCAATGT TGGGGAAATC CCCTCCGAGA 
CCATCGCCGG CTTCAGTGCC CTACAACTCA GACGAGGAAA GTCTCAAGGG CTACGATGAG 
AATCCCGACG ATAGCAGCGT GACCGAGAAG CCGTCAGAAA TGAGCTCTTC TGAATCCCAG 
GGTTCGGAAA GCGAAAACGA CAGTGTCCTC TCGAATTCGC ACTCGTTTGT GAACCACTAC 
GCCAATGTGA ACGAGCCATT GCGACCGTCC TGGAAGCGCC AAAAGCCGGT CAGGAATTTC 
TCGAGTCACA CTGATTCGGA ACCGGAAGGT TCGGCGAGTT TGAATGGAGG ACAGATTATC 
ATTAATAACA TGGCGAGGTC AAGGGCGCCC CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100142209 ( XM_015983208.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015838694.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X2, mRNA.

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CloneID	OTg21405
Clone ID Related Accession (Same CDS sequence)	XM_015983208.1
Accession Version	XM_015983208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA AGCGGAGAAA AGTGAGAAGT TTAGCGGCTA GATATGCCAG ATTGAGAAGT 
TCCGGCGAAC GCCATAGCAA AAAGAATTTT GTTTTTGTGA CACAATCTGC GGGTCCCGTG 
ATGGAATTAG GGCCTCAAAA TGCCACAGTT CTCGATGGTA AAGACGCAAC TTTAGCTTGC 
CGAGCTGTAG GAGCCCCAAC ACCCAATATC ACCTGGTTTT ACAAAGGGGA TCTTATTGAG 
ACCTCAAGCC GCGTCCAAAT TCTCGACACT GGAGACTTGC TAATAGCTGC TGTTAAGGAA 
TCAGATGTTG GAATGTACAC TTGTTTAAGA GCCAACGAAG CCGGCGAAGT ACGAGGCTCT 
GCACATTTGG GAGTCTTGGT TCGCACCCAA ATCATCCAAC CTCCGGTGGA CACGCGGGTG 
CTCCTCGGGC ATACGGCCAC TCTTCAGTGT AAAATCTCCT CCGATCCTGC TGTCCCTTTC 
GACTTGCGGT GGTACAAAAA TAAACAAGCT TTAAATCCGA AAGCGAGTTC AAGGATAAGT 
ATTTTAAAGG ATGGCACTTT GGAAATACAA GCAGTTCGAG CTGCGGATGT CGGTCTCTAC 
ACATGCTCGA TTAAATCACC TGGTGGTAAC GAAACTAGAT CGGCTAAATT GAGCGTTATC 
GAACTACCAT ACGCCCCTAC TAATGTTAAA GCCGAAAGAC TGGATACGGC CTCGCAAAGG 
GCGGTTAATG TCAGTTGGAT ACCTGGATTC GATGGAAATA GTCCGATAAA ACAATTTATT 
ATACAAAAAA GAGAGTTGCC TGAACTTGTT CCTCCGATTT CAGGCCCAAT TCCAGATCCT 
TTACTCAATT GGGTAACTGA ACTCAGTAAC GTATCAGCCG AACAAAGGTG GATTTTATTA 
ACAAATTTAA AAGCAGCTGC TGCATATCAG TTCCGAGTTA GCGCCGTTAA TAGTGTGGGA 
GAAGGAAGTC CGTCCGAACC CAGTAACCAA GTCATGTTAC CTCAAGAAGC CCCTTCTGGA 
CCTCCTGTTG GTTTCGTGGG TTCAGCCCGA AGTTCCTCCG AAATCATAAC ACAGTGGCAG 
CCACCTCTCG AAGAGCACCG TAACGGTCAA ATCCTCGGTT ACATAATCAG ATACAAATTA 
TTCGGTTACA CCAACAGTCC TTGGATCGTT CAAAACATAA CAAACGAAGC GCAAAGAAAT 
TACTTAATTC AAGATTTAAT AACTTGGAAA GATTACATCG TCCAGATCGC CGCCTACAAC 
AACAAAGGCG TTGGGGTTTT CACCGAAGGC GCCCGAATTA AGACCAAGGA GGGCGTCCCC 
GAAGCCCCCC CTATAATTCA AACGGTGGTC CCCCTGAACT CCACCACAAT CCAAATCACG 
TGGAAACCGC CAGATCCGCA AAAAATCAAC GGAATCAACC AGGGATATAA AATCCAAGCG 
TGGCAAACCG ACAAAAACGG CGACTTATTT GAAGCCAAAA TGATAACAGT TCACCCGAAT 
TTACTCGACC CGAACCAGGA ACAAACGGCG CTTATGACGG GCCTAGAGAA GTTCACCGAC 
TACAATGTTA CGGTCCTGTG CTTCACCGAC CCTGGTGATG GCGAAATCAG CGAGTTTGTC 
CCAGTGAAAA CAAAAGAAGA TGTACCTGAC GAAGTCAGCA ACTTGCAATT CGACGACATT 
AGCGACCGCG CCGTTAAAGT CATCTGGCAG CCCCCCAAGC ACATCAACGG CATCTTAACG 
GGGTACCAAA TCCGGTACCA AATTCGCGAC AAGCCCGACA CTGTCAAAAT CAAGAACCTC 
ACAGCCGATA CCCTCAGCCT CAAAGTAACC GACTTGCAAG CCACCACTCA TTACAAATTT 
GAGGTCACGG CCTGGACTGC AATGGGGGCA GGCCCCCCTA AAGTGGCGAT CATCCAGTCG 
GGGGTGGAGC CCGTCTTGCC CCATCCCCCC AACAAACTCG CCTTATCCAA CATTGAGGCC 
TTCTCTGTAG TTTTGCAATT CACCCCCGGC TTTGACGGCA ACTCCTCCAT CACCAAGTGG 
ACGGTGGAGG CCCAAACTGC GCGCAACGCC TCCTGGTTTG TCGTTTTCGA AATAAGCGAT 
CCAGACGCAA CTACGATTAC TGTTACGGGA CTTGTCCCTT TCACTGTCTA TAAACTGCGG 
TTGATTGCGA ATAATGTCGT GGGGGCTTCC GAGCCCTCCG AAGCCTCCAA GGAGTTCCAG 
ACGATCCAAG CGCCCCCAGC ACACCCCCCG AAGAACGTCA CAGTGCGGGC CATGAGCGCG 
ACTGAAGTGA GGGTTCGCTG GATTCCTCTG CAGCAAATCG AATGGTTCGG GAACCCGCGA 
GGGTACTCGA TTAGTTACGT GGAGGTGCGA ACGGGGGAAA AACAGACGGC GACAATTGAC 
GATCATACGG CCAATTCGCA TGTGATTACC GGATTGGAGG AGTTTGCGTT GTATGAAGTG 
TCCATGCAGG CGTGCAATGA CGTGGGGTGT TCCATGGACG GGCCCGTGGC TTTGGAGAGG 
ACCAGGGAGT CAGTACCATC GTTCGGGCCG TTAAACATCG AAGCGAACGC TACCTCTAGC 
ACAACAATCG TAGTGAAATG GGGTGACGTC CCGAAAGAAC ACCAAAACGG CCTAATTGAA 
GGCTACAAAG TTTATTACGC CGCCGAATCA AAATCGCCCG TACAGTACAA GCTAATCGCC 
AGCAATTCCA CTTTTACAAC CACTTTGACC GAATTGCGGA AATTCGTCGT TTACCGCATC 
CAGGTCCTGG CGTACACACG GCTCGGCGAC GGCGAGCCCA GCAAACCGGC CGTCAGCGTG 
CGAACCTTCG AAGATGTCCC GGGCCCCCCC TCGAATGTGT CATTCCCGGA CGTTTCCGTC 
ACTTCGGCCA GGATCATCTG GGACGTCCCG GAAGAACCCA ACGGCGAAAT CCTGGCGTAC 
AAAGTCACAT ATCATATCGA CAACGTGAAC GTTAATAATA AATACTCGAA GGAATTTCCG 
CCGTCTGATA GAACGTTCAG GTTTACGCAG TTGGAGCCTG AGAAGTATTA CATGTTTTCT 
GTGACTGCTC AGACCAGATT AGGTTGGGGC AAGACAGCTC ATGCTTTGGT TTTTACAACG 
AATAATCGAG ACACACCACA GTCACCATCT ATGCCACAAA TCAGTCGCAG TCAGGTACAG 
AGCAAGCAGA TCACTTTCAG CTGGACACCG GGCAGAGACG GATTCGCACC GTTGAGATAT 
TATACGGTCC AAAAAATGGA AAATGGGGGC CCTTGGCAGT CCCTTCCCGA ACGTGTGGAC 
CCTCAACTCA CCTCATACAC CGTCTCTGAC CTCAAACCCT TCACTTCATA CCGCTTCAGA 
ATCCGCGCAA CGAACGATAT AGGTCCCAGC CCCTTCAGTC CCGAATCCAT CGAAGTCCGC 
ACTTATCCAG CAGCCCCCAG CAAAGGCGTC ATGAACTTAA AAGTAGTTCC CATAACAACG 
ACAAGTGTCG AAGTCTATTG GGACCCAGTT GAGGAGAAGT ACTGGAACGG GGACGTCAAA 
ACAGGGGGCT ATAGAGTAGT CTTTCAACCC GTCTCTGACT ATCCAACTGC ACTGCAGGCA 
ACACCCAAGG AGGAAATTTT CGGAATCAAT GCCAAAAAAA TGGTTCTGAG CGAATTATTG 
CAAGACAAGA ATTATGAGAT AGTTGTGGTG CCGTTTAATT CGCAAGGGGA GGGCCCCGCA 
ACGCCCCCTG TCACAGTGTA TGTAGGGGAA GCCGTCCCAA CTGGTGAACC AAGGGACTTG 
GAGGGCGAAG CTGTTTCCTC GACGGAAGTT CGCTTGAAAT GGAAGCCGCC ACAGCAGCAG 
CAACAAAACG GCGAATTGTT GGGATACAAG ATATTTTATT TGGTGACAAA CTCGCCGCAA 
GATTTTGACG AGGGCCACAA GTTCGAGGAG GAAATCGAAG TGATTGCAGC TACGATGACC 
TCGCACAGCC TTGTCTTTCT CGACAAATAC ACCGAATATC GCATACAAAT TCTGGCGTTT 
AACCCAGCAG GTGATGGACC ACGATCGCGC CCCATCACAG TTAAGACCCT TCAAGGTCTC 
CCTGGTCCAC CAGCCAATCT ACGCTTTTCT GACATAACGA TGAATAGCTT GCTCGTGTCG 
TGGGATCCCC CGAAAAAACG CAACGGTGAC ATAATCGGAT ACCTTGTCAC GTATGAGACG 
ACCGAGGAGA ACGAACGTTT TAGTAAACAA GTCAAGCAGA AAGTGACGTC GACGGAACTC 
TTGCTACAAA CTTTGGAGGA GGAAATCACT TACACGTTCA CAGTGCGGGC ACAAACCATC 
GATTATGGGC CCTCTATTTC GGATAACGTC ACCACAGGGC CACAGGACGG CTCCCCAACG 
ACGCCAAAAG AACTCACTTT GATTAAAACA CCGTCAAGTG TGGAGCTGCA CTGGGTCAAT 
GGCTTGACCG GAAAGGGGCC AATTCTGGGC TACTATATCG AATCGAGGAA AAAAGATGAC 
CAGAGGTGGC AAACAGTCAC GAAATCGACA AACGGGCCTT TGCAAGAATT TACAGTTTCG 
TACCAAAGTC TCCTCCCCTC AACCTCTTAC TATTTCAGAG TTTTTCCTTA CAATAAATTC 
GGTATTAGTT GTCCCGCGTA CACCAAAGAC CCGGTCGTAA CACCGTCAAA ACTGTACTTG 
GAATATAGCT ACTTACAGTA TGAACCCTTC TACCGACAGA CCTGGTTCAT GGTCGCCCTG 
GCAGCGACCT CCATCATCAT TATCATAATG ATAATTGCGA TTTTGTGCGT TAAGAGCAAA 
AGTTACAAAT ATAAACAAGA AGGTGCCCAA AATAGTATGG GCGAACTCAT GGCAATGTCA 
CGCGACGACA TGACCGCTGA CTATTACCGA ACTCGCAACG GCACAAGTAA CCTAAGCTCG 
ACTCTTTCAA AACGTAGCAC TTTATCCCGC AAGCCGGTCC ATCAACAACC CCCTCCAATG 
TTGGGGAAAT CCCCTCCGAG ACCATCGCCG GCTTCAGTGC CCTACAACTC AGACGAGGAA 
AGTCTCAAGG GCTACGATGA GAATCCCGAC GATAGCAGCG TGACCGAGAA GCCGTCAGAA 
ATGAGCTCTT CTGAATCCCA GGGTTCGGAA AGCGAAAACG ACAGTGTCCT CTCGAATTCG 
CACTCGTTTG TGAACCACTA CGCCAATGTG AACGAGCCAT TGCGACCGTC CTGGAAGCGC 
CAAAAGCCGG TCAGGAATTT CTCGAGTCAC ACTGATTCGG AACCGGAAGG TTCGGCGAGT 
TTGAATGGAG GACAGATTAT CATTAATAAC ATGGCGAGGT CAAGGGCGCC CCTACCCGGA 
TTTTCGTCGT TTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015838694.1
CDS	385..7002
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKKRRKVRSLAARYARLRSSGERHSKKNFVFVTQSAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELVPPISGPIPDPLLNWVTELSNVSAEQRWILLTNLKAAAAYQFRVSAVNSVGEGSPSEPSNQVMLPQEAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQEGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_015983208.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015983208.1

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA AGCGGAGAAA AGTGAGAAGT TTAGCGGCTA GATATGCCAG ATTGAGAAGT 
TCCGGCGAAC GCCATAGCAA AAAGAATTTT GTTTTTGTGA CACAATCTGC GGGTCCCGTG 
ATGGAATTAG GGCCTCAAAA TGCCACAGTT CTCGATGGTA AAGACGCAAC TTTAGCTTGC 
CGAGCTGTAG GAGCCCCAAC ACCCAATATC ACCTGGTTTT ACAAAGGGGA TCTTATTGAG 
ACCTCAAGCC GCGTCCAAAT TCTCGACACT GGAGACTTGC TAATAGCTGC TGTTAAGGAA 
TCAGATGTTG GAATGTACAC TTGTTTAAGA GCCAACGAAG CCGGCGAAGT ACGAGGCTCT 
GCACATTTGG GAGTCTTGGT TCGCACCCAA ATCATCCAAC CTCCGGTGGA CACGCGGGTG 
CTCCTCGGGC ATACGGCCAC TCTTCAGTGT AAAATCTCCT CCGATCCTGC TGTCCCTTTC 
GACTTGCGGT GGTACAAAAA TAAACAAGCT TTAAATCCGA AAGCGAGTTC AAGGATAAGT 
ATTTTAAAGG ATGGCACTTT GGAAATACAA GCAGTTCGAG CTGCGGATGT CGGTCTCTAC 
ACATGCTCGA TTAAATCACC TGGTGGTAAC GAAACTAGAT CGGCTAAATT GAGCGTTATC 
GAACTACCAT ACGCCCCTAC TAATGTTAAA GCCGAAAGAC TGGATACGGC CTCGCAAAGG 
GCGGTTAATG TCAGTTGGAT ACCTGGATTC GATGGAAATA GTCCGATAAA ACAATTTATT 
ATACAAAAAA GAGAGTTGCC TGAACTTGTT CCTCCGATTT CAGGCCCAAT TCCAGATCCT 
TTACTCAATT GGGTAACTGA ACTCAGTAAC GTATCAGCCG AACAAAGGTG GATTTTATTA 
ACAAATTTAA AAGCAGCTGC TGCATATCAG TTCCGAGTTA GCGCCGTTAA TAGTGTGGGA 
GAAGGAAGTC CGTCCGAACC CAGTAACCAA GTCATGTTAC CTCAAGAAGC CCCTTCTGGA 
CCTCCTGTTG GTTTCGTGGG TTCAGCCCGA AGTTCCTCCG AAATCATAAC ACAGTGGCAG 
CCACCTCTCG AAGAGCACCG TAACGGTCAA ATCCTCGGTT ACATAATCAG ATACAAATTA 
TTCGGTTACA CCAACAGTCC TTGGATCGTT CAAAACATAA CAAACGAAGC GCAAAGAAAT 
TACTTAATTC AAGATTTAAT AACTTGGAAA GATTACATCG TCCAGATCGC CGCCTACAAC 
AACAAAGGCG TTGGGGTTTT CACCGAAGGC GCCCGAATTA AGACCAAGGA GGGCGTCCCC 
GAAGCCCCCC CTATAATTCA AACGGTGGTC CCCCTGAACT CCACCACAAT CCAAATCACG 
TGGAAACCGC CAGATCCGCA AAAAATCAAC GGAATCAACC AGGGATATAA AATCCAAGCG 
TGGCAAACCG ACAAAAACGG CGACTTATTT GAAGCCAAAA TGATAACAGT TCACCCGAAT 
TTACTCGACC CGAACCAGGA ACAAACGGCG CTTATGACGG GCCTAGAGAA GTTCACCGAC 
TACAATGTTA CGGTCCTGTG CTTCACCGAC CCTGGTGATG GCGAAATCAG CGAGTTTGTC 
CCAGTGAAAA CAAAAGAAGA TGTACCTGAC GAAGTCAGCA ACTTGCAATT CGACGACATT 
AGCGACCGCG CCGTTAAAGT CATCTGGCAG CCCCCCAAGC ACATCAACGG CATCTTAACG 
GGGTACCAAA TCCGGTACCA AATTCGCGAC AAGCCCGACA CTGTCAAAAT CAAGAACCTC 
ACAGCCGATA CCCTCAGCCT CAAAGTAACC GACTTGCAAG CCACCACTCA TTACAAATTT 
GAGGTCACGG CCTGGACTGC AATGGGGGCA GGCCCCCCTA AAGTGGCGAT CATCCAGTCG 
GGGGTGGAGC CCGTCTTGCC CCATCCCCCC AACAAACTCG CCTTATCCAA CATTGAGGCC 
TTCTCTGTAG TTTTGCAATT CACCCCCGGC TTTGACGGCA ACTCCTCCAT CACCAAGTGG 
ACGGTGGAGG CCCAAACTGC GCGCAACGCC TCCTGGTTTG TCGTTTTCGA AATAAGCGAT 
CCAGACGCAA CTACGATTAC TGTTACGGGA CTTGTCCCTT TCACTGTCTA TAAACTGCGG 
TTGATTGCGA ATAATGTCGT GGGGGCTTCC GAGCCCTCCG AAGCCTCCAA GGAGTTCCAG 
ACGATCCAAG CGCCCCCAGC ACACCCCCCG AAGAACGTCA CAGTGCGGGC CATGAGCGCG 
ACTGAAGTGA GGGTTCGCTG GATTCCTCTG CAGCAAATCG AATGGTTCGG GAACCCGCGA 
GGGTACTCGA TTAGTTACGT GGAGGTGCGA ACGGGGGAAA AACAGACGGC GACAATTGAC 
GATCATACGG CCAATTCGCA TGTGATTACC GGATTGGAGG AGTTTGCGTT GTATGAAGTG 
TCCATGCAGG CGTGCAATGA CGTGGGGTGT TCCATGGACG GGCCCGTGGC TTTGGAGAGG 
ACCAGGGAGT CAGTACCATC GTTCGGGCCG TTAAACATCG AAGCGAACGC TACCTCTAGC 
ACAACAATCG TAGTGAAATG GGGTGACGTC CCGAAAGAAC ACCAAAACGG CCTAATTGAA 
GGCTACAAAG TTTATTACGC CGCCGAATCA AAATCGCCCG TACAGTACAA GCTAATCGCC 
AGCAATTCCA CTTTTACAAC CACTTTGACC GAATTGCGGA AATTCGTCGT TTACCGCATC 
CAGGTCCTGG CGTACACACG GCTCGGCGAC GGCGAGCCCA GCAAACCGGC CGTCAGCGTG 
CGAACCTTCG AAGATGTCCC GGGCCCCCCC TCGAATGTGT CATTCCCGGA CGTTTCCGTC 
ACTTCGGCCA GGATCATCTG GGACGTCCCG GAAGAACCCA ACGGCGAAAT CCTGGCGTAC 
AAAGTCACAT ATCATATCGA CAACGTGAAC GTTAATAATA AATACTCGAA GGAATTTCCG 
CCGTCTGATA GAACGTTCAG GTTTACGCAG TTGGAGCCTG AGAAGTATTA CATGTTTTCT 
GTGACTGCTC AGACCAGATT AGGTTGGGGC AAGACAGCTC ATGCTTTGGT TTTTACAACG 
AATAATCGAG ACACACCACA GTCACCATCT ATGCCACAAA TCAGTCGCAG TCAGGTACAG 
AGCAAGCAGA TCACTTTCAG CTGGACACCG GGCAGAGACG GATTCGCACC GTTGAGATAT 
TATACGGTCC AAAAAATGGA AAATGGGGGC CCTTGGCAGT CCCTTCCCGA ACGTGTGGAC 
CCTCAACTCA CCTCATACAC CGTCTCTGAC CTCAAACCCT TCACTTCATA CCGCTTCAGA 
ATCCGCGCAA CGAACGATAT AGGTCCCAGC CCCTTCAGTC CCGAATCCAT CGAAGTCCGC 
ACTTATCCAG CAGCCCCCAG CAAAGGCGTC ATGAACTTAA AAGTAGTTCC CATAACAACG 
ACAAGTGTCG AAGTCTATTG GGACCCAGTT GAGGAGAAGT ACTGGAACGG GGACGTCAAA 
ACAGGGGGCT ATAGAGTAGT CTTTCAACCC GTCTCTGACT ATCCAACTGC ACTGCAGGCA 
ACACCCAAGG AGGAAATTTT CGGAATCAAT GCCAAAAAAA TGGTTCTGAG CGAATTATTG 
CAAGACAAGA ATTATGAGAT AGTTGTGGTG CCGTTTAATT CGCAAGGGGA GGGCCCCGCA 
ACGCCCCCTG TCACAGTGTA TGTAGGGGAA GCCGTCCCAA CTGGTGAACC AAGGGACTTG 
GAGGGCGAAG CTGTTTCCTC GACGGAAGTT CGCTTGAAAT GGAAGCCGCC ACAGCAGCAG 
CAACAAAACG GCGAATTGTT GGGATACAAG ATATTTTATT TGGTGACAAA CTCGCCGCAA 
GATTTTGACG AGGGCCACAA GTTCGAGGAG GAAATCGAAG TGATTGCAGC TACGATGACC 
TCGCACAGCC TTGTCTTTCT CGACAAATAC ACCGAATATC GCATACAAAT TCTGGCGTTT 
AACCCAGCAG GTGATGGACC ACGATCGCGC CCCATCACAG TTAAGACCCT TCAAGGTCTC 
CCTGGTCCAC CAGCCAATCT ACGCTTTTCT GACATAACGA TGAATAGCTT GCTCGTGTCG 
TGGGATCCCC CGAAAAAACG CAACGGTGAC ATAATCGGAT ACCTTGTCAC GTATGAGACG 
ACCGAGGAGA ACGAACGTTT TAGTAAACAA GTCAAGCAGA AAGTGACGTC GACGGAACTC 
TTGCTACAAA CTTTGGAGGA GGAAATCACT TACACGTTCA CAGTGCGGGC ACAAACCATC 
GATTATGGGC CCTCTATTTC GGATAACGTC ACCACAGGGC CACAGGACGG CTCCCCAACG 
ACGCCAAAAG AACTCACTTT GATTAAAACA CCGTCAAGTG TGGAGCTGCA CTGGGTCAAT 
GGCTTGACCG GAAAGGGGCC AATTCTGGGC TACTATATCG AATCGAGGAA AAAAGATGAC 
CAGAGGTGGC AAACAGTCAC GAAATCGACA AACGGGCCTT TGCAAGAATT TACAGTTTCG 
TACCAAAGTC TCCTCCCCTC AACCTCTTAC TATTTCAGAG TTTTTCCTTA CAATAAATTC 
GGTATTAGTT GTCCCGCGTA CACCAAAGAC CCGGTCGTAA CACCGTCAAA ACTGTACTTG 
GAATATAGCT ACTTACAGTA TGAACCCTTC TACCGACAGA CCTGGTTCAT GGTCGCCCTG 
GCAGCGACCT CCATCATCAT TATCATAATG ATAATTGCGA TTTTGTGCGT TAAGAGCAAA 
AGTTACAAAT ATAAACAAGA AGGTGCCCAA AATAGTATGG GCGAACTCAT GGCAATGTCA 
CGCGACGACA TGACCGCTGA CTATTACCGA ACTCGCAACG GCACAAGTAA CCTAAGCTCG 
ACTCTTTCAA AACGTAGCAC TTTATCCCGC AAGCCGGTCC ATCAACAACC CCCTCCAATG 
TTGGGGAAAT CCCCTCCGAG ACCATCGCCG GCTTCAGTGC CCTACAACTC AGACGAGGAA 
AGTCTCAAGG GCTACGATGA GAATCCCGAC GATAGCAGCG TGACCGAGAA GCCGTCAGAA 
ATGAGCTCTT CTGAATCCCA GGGTTCGGAA AGCGAAAACG ACAGTGTCCT CTCGAATTCG 
CACTCGTTTG TGAACCACTA CGCCAATGTG AACGAGCCAT TGCGACCGTC CTGGAAGCGC 
CAAAAGCCGG TCAGGAATTT CTCGAGTCAC ACTGATTCGG AACCGGAAGG TTCGGCGAGT 
TTGAATGGAG GACAGATTAT CATTAATAAC ATGGCGAGGT CAAGGGCGCC CCTACCCGGA 
TTTTCGTCGT TTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100142209 ( XM_015983209.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015838695.1 Tribolium castaneum protein sidekick (LOC100142209), transcript variant X6, mRNA.

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CloneID	OTg21406
Clone ID Related Accession (Same CDS sequence)	XM_015983209.1
Accession Version	XM_015983209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein sidekick isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA AGCGGAGAAA AGTGAGAAGT TTAGCGGCTA GATATGCCAG ATTGAGAAGT 
TCCGGCGAAC GCCATAGCAA AAAGAATTTT GTTTTTGTGA CACAATCTGC GGGTCCCGTG 
ATGGAATTAG GGCCTCAAAA TGCCACAGTT CTCGATGGTA AAGACGCAAC TTTAGCTTGC 
CGAGCTGTAG GAGCCCCAAC ACCCAATATC ACCTGGTTTT ACAAAGGGGA TCTTATTGAG 
ACCTCAAGCC GCGTCCAAAT TCTCGACACT GGAGACTTGC TAATAGCTGC TGTTAAGGAA 
TCAGATGTTG GAATGTACAC TTGTTTAAGA GCCAACGAAG CCGGCGAAGT ACGAGGCTCT 
GCACATTTGG GAGTCTTGGT TCGCACCCAA ATCATCCAAC CTCCGGTGGA CACGCGGGTG 
CTCCTCGGGC ATACGGCCAC TCTTCAGTGT AAAATCTCCT CCGATCCTGC TGTCCCTTTC 
GACTTGCGGT GGTACAAAAA TAAACAAGCT TTAAATCCGA AAGCGAGTTC AAGGATAAGT 
ATTTTAAAGG ATGGCACTTT GGAAATACAA GCAGTTCGAG CTGCGGATGT CGGTCTCTAC 
ACATGCTCGA TTAAATCACC TGGTGGTAAC GAAACTAGAT CGGCTAAATT GAGCGTTATC 
GAACTACCAT ACGCCCCTAC TAATGTTAAA GCCGAAAGAC TGGATACGGC CTCGCAAAGG 
GCGGTTAATG TCAGTTGGAT ACCTGGATTC GATGGAAATA GTCCGATAAA ACAATTTATT 
ATACAAAAAA GAGAGTTGCC TGAACTTGCC CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT 
TCAGCCCGAA GTTCCTCCGA AATCATAACA CAGTGGCAGC CACCTCTCGA AGAGCACCGT 
AACGGTCAAA TCCTCGGTTA CATAATCAGA TACAAATTAT TCGGTTACAC CAACAGTCCT 
TGGATCGTTC AAAACATAAC AAACGAAGCG CAAAGAAATT ACTTAATTCA AGATTTAATA 
ACTTGGAAAG ATTACATCGT CCAGATCGCC GCCTACAACA ACAAAGGCGT TGGGGTTTTC 
ACCGAAGGCG CCCGAATTAA GACCAAGGAG GGCGTCCCCG AAGCCCCCCC TATAATTCAA 
ACGGTGGTCC CCCTGAACTC CACCACAATC CAAATCACGT GGAAACCGCC AGATCCGCAA 
AAAATCAACG GAATCAACCA GGGATATAAA ATCCAAGCGT GGCAAACCGA CAAAAACGGC 
GACTTATTTG AAGCCAAAAT GATAACAGTT CACCCGAATT TACTCGACCC GAACCAGGAA 
CAAACGGCGC TTATGACGGG CCTAGAGAAG TTCACCGACT ACAATGTTAC GGTCCTGTGC 
TTCACCGACC CTGGTGATGG CGAAATCAGC GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT 
GTACCTGACG AAGTCAGCAA CTTGCAATTC GACGACATTA GCGACCGCGC CGTTAAAGTC 
ATCTGGCAGC CCCCCAAGCA CATCAACGGC ATCTTAACGG GGTACCAAAT CCGGTACCAA 
ATTCGCGACA AGCCCGACAC TGTCAAAATC AAGAACCTCA CAGCCGATAC CCTCAGCCTC 
AAAGTAACCG ACTTGCAAGC CACCACTCAT TACAAATTTG AGGTCACGGC CTGGACTGCA 
ATGGGGGCAG GCCCCCCTAA AGTGGCGATC ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC 
CATCCCCCCA ACAAACTCGC CTTATCCAAC ATTGAGGCCT TCTCTGTAGT TTTGCAATTC 
ACCCCCGGCT TTGACGGCAA CTCCTCCATC ACCAAGTGGA CGGTGGAGGC CCAAACTGCG 
CGCAACGCCT CCTGGTTTGT CGTTTTCGAA ATAAGCGATC CAGACGCAAC TACGATTACT 
GTTACGGGAC TTGTCCCTTT CACTGTCTAT AAACTGCGGT TGATTGCGAA TAATGTCGTG 
GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG GAGTTCCAGA CGATCCAAGC GCCCCCAGCA 
CACCCCCCGA AGAACGTCAC AGTGCGGGCC ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG 
ATTCCTCTGC AGCAAATCGA ATGGTTCGGG AACCCGCGAG GGTACTCGAT TAGTTACGTG 
GAGGTGCGAA CGGGGGAAAA ACAGACGGCG ACAATTGACG ATCATACGGC CAATTCGCAT 
GTGATTACCG GATTGGAGGA GTTTGCGTTG TATGAAGTGT CCATGCAGGC GTGCAATGAC 
GTGGGGTGTT CCATGGACGG GCCCGTGGCT TTGGAGAGGA CCAGGGAGTC AGTACCATCG 
TTCGGGCCGT TAAACATCGA AGCGAACGCT ACCTCTAGCA CAACAATCGT AGTGAAATGG 
GGTGACGTCC CGAAAGAACA CCAAAACGGC CTAATTGAAG GCTACAAAGT TTATTACGCC 
GCCGAATCAA AATCGCCCGT ACAGTACAAG CTAATCGCCA GCAATTCCAC TTTTACAACC 
ACTTTGACCG AATTGCGGAA ATTCGTCGTT TACCGCATCC AGGTCCTGGC GTACACACGG 
CTCGGCGACG GCGAGCCCAG CAAACCGGCC GTCAGCGTGC GAACCTTCGA AGATGTCCCG 
GGCCCCCCCT CGAATGTGTC ATTCCCGGAC GTTTCCGTCA CTTCGGCCAG GATCATCTGG 
GACGTCCCGG AAGAACCCAA CGGCGAAATC CTGGCGTACA AAGTCACATA TCATATCGAC 
AACGTGAACG TTAATAATAA ATACTCGAAG GAATTTCCGC CGTCTGATAG AACGTTCAGG 
TTTACGCAGT TGGAGCCTGA GAAGTATTAC ATGTTTTCTG TGACTGCTCA GACCAGATTA 
GGTTGGGGCA AGACAGCTCA TGCTTTGGTT TTTACAACGA ATAATCGAGA CACACCACAG 
TCACCATCTA TGCCACAAAT CAGTCGCAGT CAGGTACAGA GCAAGCAGAT CACTTTCAGC 
TGGACACCGG GCAGAGACGG ATTCGCACCG TTGAGATATT ATACGGTCCA AAAAATGGAA 
AATGGGGGCC CTTGGCAGTC CCTTCCCGAA CGTGTGGACC CTCAACTCAC CTCATACACC 
GTCTCTGACC TCAAACCCTT CACTTCATAC CGCTTCAGAA TCCGCGCAAC GAACGATATA 
GGTCCCAGCC CCTTCAGTCC CGAATCCATC GAAGTCCGCA CTTATCCAGC AGCCCCCAGC 
AAAGGCGTCA TGAACTTAAA AGTAGTTCCC ATAACAACGA CAAGTGTCGA AGTCTATTGG 
GACCCAGTTG AGGAGAAGTA CTGGAACGGG GACGTCAAAA CAGGGGGCTA TAGAGTAGTC 
TTTCAACCCG TCTCTGACTA TCCAACTGCA CTGCAGGCAA CACCCAAGGA GGAAATTTTC 
GGAATCAATG CCAAAAAAAT GGTTCTGAGC GAATTATTGC AAGACAAGAA TTATGAGATA 
GTTGTGGTGC CGTTTAATTC GCAAGGGGAG GGCCCCGCAA CGCCCCCTGT CACAGTGTAT 
GTAGGGGAAG CCGTCCCAAC TGGTGAACCA AGGGACTTGG AGGGCGAAGC TGTTTCCTCG 
ACGGAAGTTC GCTTGAAATG GAAGCCGCCA CAGCAGCAGC AACAAAACGG CGAATTGTTG 
GGATACAAGA TATTTTATTT GGTGACAAAC TCGCCGCAAG ATTTTGACGA GGGCCACAAG 
TTCGAGGAGG AAATCGAAGT GATTGCAGCT ACGATGACCT CGCACAGCCT TGTCTTTCTC 
GACAAATACA CCGAATATCG CATACAAATT CTGGCGTTTA ACCCAGCAGG TGATGGACCA 
CGATCGCGCC CCATCACAGT TAAGACCCTT CAAGGTCTCC CTGGTCCACC AGCCAATCTA 
CGCTTTTCTG ACATAACGAT GAATAGCTTG CTCGTGTCGT GGGATCCCCC GAAAAAACGC 
AACGGTGACA TAATCGGATA CCTTGTCACG TATGAGACGA CCGAGGAGAA CGAACGTTTT 
AGTAAACAAG TCAAGCAGAA AGTGACGTCG ACGGAACTCT TGCTACAAAC TTTGGAGGAG 
GAAATCACTT ACACGTTCAC AGTGCGGGCA CAAACCATCG ATTATGGGCC CTCTATTTCG 
GATAACGTCA CCACAGGGCC ACAGGACGGC TCCCCAACGA CGCCAAAAGA ACTCACTTTG 
ATTAAAACAC CGTCAAGTGT GGAGCTGCAC TGGGTCAATG GCTTGACCGG AAAGGGGCCA 
ATTCTGGGCT ACTATATCGA ATCGAGGAAA AAAGATGACC AGAGGTGGCA AACAGTCACG 
AAATCGACAA ACGGGCCTTT GCAAGAATTT ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA 
ACCTCTTACT ATTTCAGAGT TTTTCCTTAC AATAAATTCG GTATTAGTTG TCCCGCGTAC 
ACCAAAGACC CGGTCGTAAC ACCGTCAAAA CTGTACTTGG AATATAGCTA CTTACAGTAT 
GAACCCTTCT ACCGACAGAC CTGGTTCATG GTCGCCCTGG CAGCGACCTC CATCATCATT 
ATCATAATGA TAATTGCGAT TTTGTGCGTT AAGAGCAAAA GTTACAAATA TAAACAAGAA 
GGTGCCCAAA ATAGTATGGG CGAACTCATG GCAATGTCAC GCGACGACAT GACCGCTGAC 
TATTACCGAA CTCGCAACGG CACAAGTAAC CTAAGCTCGA CTCTTTCAAA ACGTAGCACT 
TTATCCCGCA AGCCGGTCCA TCAACAACCC CCTCCAATGT TGGGGAAATC CCCTCCGAGA 
CCATCGCCGG CTTCAGTGCC CTACAACTCA GACGAGGAAA GTCTCAAGGG CTACGATGAG 
AATCCCGACG ATAGCAGCGT GACCGAGAAG CCGTCAGAAA TGAGCTCTTC TGAATCCCAG 
GGTTCGGAAA GCGAAAACGA CAGTGTCCTC TCGAATTCGC ACTCGTTTGT GAACCACTAC 
GCCAATGTGA ACGAGCCATT GCGACCGTCC TGGAAGCGCC AAAAGCCGGT CAGGAATTTC 
TCGAGTCACA CTGATTCGGA ACCGGAAGGT TCGGCGAGTT TGAATGGAGG ACAGATTATC 
ATTAATAACA TGGCGAGGTC AAGGGCGCCC CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015838695.1
CDS	385..6801
Translation	MLVKRCTMEEKERKTRHMFLGIKTYLFIVCLASVPHDALAENSLQAPRFTTSSASSSIIRLGTTKIIQCQAFGNPSPQFRWLKDWVPITEFSTDAYYKIISASHKDEGSYRCIAANKIGSILSEEMKILVAYMKVFEDQTEKSESVVEGQAAILNLPEIESKPKPEVTWHTESGQLPYAQKYAKSKSNQLIILSTDKSDQKAYRARAINTQEGKEENSAFIRLTVQESSDHKEIAPQIIVPPEDVKIVRGTAQTTLDCIANARPLHELETLWFKDDILIENGGMYSLNDIWNRSLTLISVNTTQSGQYECRVRLRSERFDTVTAKAIVTVLEKPRFVSATRAETLGEYGTPISLPCDVVGIPKPNVTWYKNSQEVDLTDKRYVLEEDNSLRIKKLLINDSGMFQCFAKSEAGESSISTWLKVKKRRKVRSLAARYARLRSSGERHSKKNFVFVTQSAGPVMELGPQNATVLDGKDATLACRAVGAPTPNITWFYKGDLIETSSRVQILDTGDLLIAAVKESDVGMYTCLRANEAGEVRGSAHLGVLVRTQIIQPPVDTRVLLGHTATLQCKISSDPAVPFDLRWYKNKQALNPKASSRISILKDGTLEIQAVRAADVGLYTCSIKSPGGNETRSAKLSVIELPYAPTNVKAERLDTASQRAVNVSWIPGFDGNSPIKQFIIQKRELPELAPSGPPVGFVGSARSSSEIITQWQPPLEEHRNGQILGYIIRYKLFGYTNSPWIVQNITNEAQRNYLIQDLITWKDYIVQIAAYNNKGVGVFTEGARIKTKEGVPEAPPIIQTVVPLNSTTIQITWKPPDPQKINGINQGYKIQAWQTDKNGDLFEAKMITVHPNLLDPNQEQTALMTGLEKFTDYNVTVLCFTDPGDGEISEFVPVKTKEDVPDEVSNLQFDDISDRAVKVIWQPPKHINGILTGYQIRYQIRDKPDTVKIKNLTADTLSLKVTDLQATTHYKFEVTAWTAMGAGPPKVAIIQSGVEPVLPHPPNKLALSNIEAFSVVLQFTPGFDGNSSITKWTVEAQTARNASWFVVFEISDPDATTITVTGLVPFTVYKLRLIANNVVGASEPSEASKEFQTIQAPPAHPPKNVTVRAMSATEVRVRWIPLQQIEWFGNPRGYSISYVEVRTGEKQTATIDDHTANSHVITGLEEFALYEVSMQACNDVGCSMDGPVALERTRESVPSFGPLNIEANATSSTTIVVKWGDVPKEHQNGLIEGYKVYYAAESKSPVQYKLIASNSTFTTTLTELRKFVVYRIQVLAYTRLGDGEPSKPAVSVRTFEDVPGPPSNVSFPDVSVTSARIIWDVPEEPNGEILAYKVTYHIDNVNVNNKYSKEFPPSDRTFRFTQLEPEKYYMFSVTAQTRLGWGKTAHALVFTTNNRDTPQSPSMPQISRSQVQSKQITFSWTPGRDGFAPLRYYTVQKMENGGPWQSLPERVDPQLTSYTVSDLKPFTSYRFRIRATNDIGPSPFSPESIEVRTYPAAPSKGVMNLKVVPITTTSVEVYWDPVEEKYWNGDVKTGGYRVVFQPVSDYPTALQATPKEEIFGINAKKMVLSELLQDKNYEIVVVPFNSQGEGPATPPVTVYVGEAVPTGEPRDLEGEAVSSTEVRLKWKPPQQQQQNGELLGYKIFYLVTNSPQDFDEGHKFEEEIEVIAATMTSHSLVFLDKYTEYRIQILAFNPAGDGPRSRPITVKTLQGLPGPPANLRFSDITMNSLLVSWDPPKKRNGDIIGYLVTYETTEENERFSKQVKQKVTSTELLLQTLEEEITYTFTVRAQTIDYGPSISDNVTTGPQDGSPTTPKELTLIKTPSSVELHWVNGLTGKGPILGYYIESRKKDDQRWQTVTKSTNGPLQEFTVSYQSLLPSTSYYFRVFPYNKFGISCPAYTKDPVVTPSKLYLEYSYLQYEPFYRQTWFMVALAATSIIIIIMIIAILCVKSKSYKYKQEGAQNSMGELMAMSRDDMTADYYRTRNGTSNLSSTLSKRSTLSRKPVHQQPPPMLGKSPPRPSPASVPYNSDEESLKGYDENPDDSSVTEKPSEMSSSESQGSESENDSVLSNSHSFVNHYANVNEPLRPSWKRQKPVRNFSSHTDSEPEGSASLNGGQIIINNMARSRAPLPGFSSFV

Target ORF information:

RefSeq Version	XM_015983209.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein sidekick (LOC100142209), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015983209.1

ORF Insert Sequence:

ATGTTGGTGA AGAGGTGCAC CATGGAGGAG AAGGAGCGGA AAACACGACA CATGTTTTTA 
GGGATTAAAA CTTATTTATT TATTGTATGT TTAGCCAGTG TTCCTCACGA TGCTTTAGCT 
GAAAATTCAC TGCAAGCTCC AAGATTCACG ACGTCGTCGG CTAGCAGTAG TATTATTAGA 
TTAGGAACGA CAAAAATTAT CCAGTGCCAA GCCTTTGGCA ATCCCTCGCC GCAGTTTAGG 
TGGCTGAAAG ACTGGGTGCC AATAACTGAA TTTTCAACCG ATGCTTACTA CAAAATCATC 
TCCGCTAGTC ATAAGGATGA GGGCAGCTAC AGGTGTATAG CAGCCAACAA AATCGGCTCA 
ATACTCAGCG AGGAAATGAA GATTCTCGTA GCGTACATGA AGGTTTTCGA AGACCAGACT 
GAAAAGAGCG AGTCCGTCGT CGAAGGGCAA GCTGCAATTT TAAATCTTCC GGAAATCGAG 
TCGAAGCCTA AGCCTGAGGT GACCTGGCAC ACCGAAAGCG GTCAATTACC ATACGCTCAA 
AAATACGCCA AATCGAAATC CAACCAGCTT ATCATCCTAT CGACTGACAA GTCCGACCAG 
AAAGCGTACA GGGCGCGTGC AATAAACACC CAAGAGGGCA AGGAAGAGAA CAGCGCTTTT 
ATAAGACTGA CCGTGCAAGA ATCGAGCGAT CACAAGGAAA TCGCGCCGCA AATTATTGTC 
CCTCCCGAAG ACGTGAAAAT CGTCAGAGGG ACAGCTCAGA CAACTTTGGA TTGCATCGCC 
AACGCCAGGC CGCTGCACGA ATTGGAGACG TTGTGGTTCA AGGATGATAT TTTGATAGAG 
AATGGGGGAA TGTACTCGCT GAACGATATT TGGAACAGGA GTTTGACGTT AATTTCGGTT 
AACACCACCC AAAGTGGACA ATATGAATGC CGTGTTAGGC TGAGATCGGA ACGGTTTGAT 
ACTGTCACAG CTAAAGCGAT TGTTACTGTA CTTGAAAAAC CGAGATTTGT GAGCGCCACA 
AGGGCCGAAA CGTTGGGCGA GTACGGAACA CCCATTAGCC TACCTTGCGA TGTGGTTGGA 
ATACCGAAAC CCAATGTTAC GTGGTATAAA AACAGCCAAG AGGTTGATCT GACCGACAAG 
AGGTACGTCC TCGAAGAAGA CAATTCATTA CGTATTAAAA AGCTGTTAAT AAACGACAGC 
GGCATGTTTC AATGTTTTGC CAAAAGCGAG GCAGGGGAAA GTTCAATTTC GACATGGTTA 
AAGGTGAAAA AGCGGAGAAA AGTGAGAAGT TTAGCGGCTA GATATGCCAG ATTGAGAAGT 
TCCGGCGAAC GCCATAGCAA AAAGAATTTT GTTTTTGTGA CACAATCTGC GGGTCCCGTG 
ATGGAATTAG GGCCTCAAAA TGCCACAGTT CTCGATGGTA AAGACGCAAC TTTAGCTTGC 
CGAGCTGTAG GAGCCCCAAC ACCCAATATC ACCTGGTTTT ACAAAGGGGA TCTTATTGAG 
ACCTCAAGCC GCGTCCAAAT TCTCGACACT GGAGACTTGC TAATAGCTGC TGTTAAGGAA 
TCAGATGTTG GAATGTACAC TTGTTTAAGA GCCAACGAAG CCGGCGAAGT ACGAGGCTCT 
GCACATTTGG GAGTCTTGGT TCGCACCCAA ATCATCCAAC CTCCGGTGGA CACGCGGGTG 
CTCCTCGGGC ATACGGCCAC TCTTCAGTGT AAAATCTCCT CCGATCCTGC TGTCCCTTTC 
GACTTGCGGT GGTACAAAAA TAAACAAGCT TTAAATCCGA AAGCGAGTTC AAGGATAAGT 
ATTTTAAAGG ATGGCACTTT GGAAATACAA GCAGTTCGAG CTGCGGATGT CGGTCTCTAC 
ACATGCTCGA TTAAATCACC TGGTGGTAAC GAAACTAGAT CGGCTAAATT GAGCGTTATC 
GAACTACCAT ACGCCCCTAC TAATGTTAAA GCCGAAAGAC TGGATACGGC CTCGCAAAGG 
GCGGTTAATG TCAGTTGGAT ACCTGGATTC GATGGAAATA GTCCGATAAA ACAATTTATT 
ATACAAAAAA GAGAGTTGCC TGAACTTGCC CCTTCTGGAC CTCCTGTTGG TTTCGTGGGT 
TCAGCCCGAA GTTCCTCCGA AATCATAACA CAGTGGCAGC CACCTCTCGA AGAGCACCGT 
AACGGTCAAA TCCTCGGTTA CATAATCAGA TACAAATTAT TCGGTTACAC CAACAGTCCT 
TGGATCGTTC AAAACATAAC AAACGAAGCG CAAAGAAATT ACTTAATTCA AGATTTAATA 
ACTTGGAAAG ATTACATCGT CCAGATCGCC GCCTACAACA ACAAAGGCGT TGGGGTTTTC 
ACCGAAGGCG CCCGAATTAA GACCAAGGAG GGCGTCCCCG AAGCCCCCCC TATAATTCAA 
ACGGTGGTCC CCCTGAACTC CACCACAATC CAAATCACGT GGAAACCGCC AGATCCGCAA 
AAAATCAACG GAATCAACCA GGGATATAAA ATCCAAGCGT GGCAAACCGA CAAAAACGGC 
GACTTATTTG AAGCCAAAAT GATAACAGTT CACCCGAATT TACTCGACCC GAACCAGGAA 
CAAACGGCGC TTATGACGGG CCTAGAGAAG TTCACCGACT ACAATGTTAC GGTCCTGTGC 
TTCACCGACC CTGGTGATGG CGAAATCAGC GAGTTTGTCC CAGTGAAAAC AAAAGAAGAT 
GTACCTGACG AAGTCAGCAA CTTGCAATTC GACGACATTA GCGACCGCGC CGTTAAAGTC 
ATCTGGCAGC CCCCCAAGCA CATCAACGGC ATCTTAACGG GGTACCAAAT CCGGTACCAA 
ATTCGCGACA AGCCCGACAC TGTCAAAATC AAGAACCTCA CAGCCGATAC CCTCAGCCTC 
AAAGTAACCG ACTTGCAAGC CACCACTCAT TACAAATTTG AGGTCACGGC CTGGACTGCA 
ATGGGGGCAG GCCCCCCTAA AGTGGCGATC ATCCAGTCGG GGGTGGAGCC CGTCTTGCCC 
CATCCCCCCA ACAAACTCGC CTTATCCAAC ATTGAGGCCT TCTCTGTAGT TTTGCAATTC 
ACCCCCGGCT TTGACGGCAA CTCCTCCATC ACCAAGTGGA CGGTGGAGGC CCAAACTGCG 
CGCAACGCCT CCTGGTTTGT CGTTTTCGAA ATAAGCGATC CAGACGCAAC TACGATTACT 
GTTACGGGAC TTGTCCCTTT CACTGTCTAT AAACTGCGGT TGATTGCGAA TAATGTCGTG 
GGGGCTTCCG AGCCCTCCGA AGCCTCCAAG GAGTTCCAGA CGATCCAAGC GCCCCCAGCA 
CACCCCCCGA AGAACGTCAC AGTGCGGGCC ATGAGCGCGA CTGAAGTGAG GGTTCGCTGG 
ATTCCTCTGC AGCAAATCGA ATGGTTCGGG AACCCGCGAG GGTACTCGAT TAGTTACGTG 
GAGGTGCGAA CGGGGGAAAA ACAGACGGCG ACAATTGACG ATCATACGGC CAATTCGCAT 
GTGATTACCG GATTGGAGGA GTTTGCGTTG TATGAAGTGT CCATGCAGGC GTGCAATGAC 
GTGGGGTGTT CCATGGACGG GCCCGTGGCT TTGGAGAGGA CCAGGGAGTC AGTACCATCG 
TTCGGGCCGT TAAACATCGA AGCGAACGCT ACCTCTAGCA CAACAATCGT AGTGAAATGG 
GGTGACGTCC CGAAAGAACA CCAAAACGGC CTAATTGAAG GCTACAAAGT TTATTACGCC 
GCCGAATCAA AATCGCCCGT ACAGTACAAG CTAATCGCCA GCAATTCCAC TTTTACAACC 
ACTTTGACCG AATTGCGGAA ATTCGTCGTT TACCGCATCC AGGTCCTGGC GTACACACGG 
CTCGGCGACG GCGAGCCCAG CAAACCGGCC GTCAGCGTGC GAACCTTCGA AGATGTCCCG 
GGCCCCCCCT CGAATGTGTC ATTCCCGGAC GTTTCCGTCA CTTCGGCCAG GATCATCTGG 
GACGTCCCGG AAGAACCCAA CGGCGAAATC CTGGCGTACA AAGTCACATA TCATATCGAC 
AACGTGAACG TTAATAATAA ATACTCGAAG GAATTTCCGC CGTCTGATAG AACGTTCAGG 
TTTACGCAGT TGGAGCCTGA GAAGTATTAC ATGTTTTCTG TGACTGCTCA GACCAGATTA 
GGTTGGGGCA AGACAGCTCA TGCTTTGGTT TTTACAACGA ATAATCGAGA CACACCACAG 
TCACCATCTA TGCCACAAAT CAGTCGCAGT CAGGTACAGA GCAAGCAGAT CACTTTCAGC 
TGGACACCGG GCAGAGACGG ATTCGCACCG TTGAGATATT ATACGGTCCA AAAAATGGAA 
AATGGGGGCC CTTGGCAGTC CCTTCCCGAA CGTGTGGACC CTCAACTCAC CTCATACACC 
GTCTCTGACC TCAAACCCTT CACTTCATAC CGCTTCAGAA TCCGCGCAAC GAACGATATA 
GGTCCCAGCC CCTTCAGTCC CGAATCCATC GAAGTCCGCA CTTATCCAGC AGCCCCCAGC 
AAAGGCGTCA TGAACTTAAA AGTAGTTCCC ATAACAACGA CAAGTGTCGA AGTCTATTGG 
GACCCAGTTG AGGAGAAGTA CTGGAACGGG GACGTCAAAA CAGGGGGCTA TAGAGTAGTC 
TTTCAACCCG TCTCTGACTA TCCAACTGCA CTGCAGGCAA CACCCAAGGA GGAAATTTTC 
GGAATCAATG CCAAAAAAAT GGTTCTGAGC GAATTATTGC AAGACAAGAA TTATGAGATA 
GTTGTGGTGC CGTTTAATTC GCAAGGGGAG GGCCCCGCAA CGCCCCCTGT CACAGTGTAT 
GTAGGGGAAG CCGTCCCAAC TGGTGAACCA AGGGACTTGG AGGGCGAAGC TGTTTCCTCG 
ACGGAAGTTC GCTTGAAATG GAAGCCGCCA CAGCAGCAGC AACAAAACGG CGAATTGTTG 
GGATACAAGA TATTTTATTT GGTGACAAAC TCGCCGCAAG ATTTTGACGA GGGCCACAAG 
TTCGAGGAGG AAATCGAAGT GATTGCAGCT ACGATGACCT CGCACAGCCT TGTCTTTCTC 
GACAAATACA CCGAATATCG CATACAAATT CTGGCGTTTA ACCCAGCAGG TGATGGACCA 
CGATCGCGCC CCATCACAGT TAAGACCCTT CAAGGTCTCC CTGGTCCACC AGCCAATCTA 
CGCTTTTCTG ACATAACGAT GAATAGCTTG CTCGTGTCGT GGGATCCCCC GAAAAAACGC 
AACGGTGACA TAATCGGATA CCTTGTCACG TATGAGACGA CCGAGGAGAA CGAACGTTTT 
AGTAAACAAG TCAAGCAGAA AGTGACGTCG ACGGAACTCT TGCTACAAAC TTTGGAGGAG 
GAAATCACTT ACACGTTCAC AGTGCGGGCA CAAACCATCG ATTATGGGCC CTCTATTTCG 
GATAACGTCA CCACAGGGCC ACAGGACGGC TCCCCAACGA CGCCAAAAGA ACTCACTTTG 
ATTAAAACAC CGTCAAGTGT GGAGCTGCAC TGGGTCAATG GCTTGACCGG AAAGGGGCCA 
ATTCTGGGCT ACTATATCGA ATCGAGGAAA AAAGATGACC AGAGGTGGCA AACAGTCACG 
AAATCGACAA ACGGGCCTTT GCAAGAATTT ACAGTTTCGT ACCAAAGTCT CCTCCCCTCA 
ACCTCTTACT ATTTCAGAGT TTTTCCTTAC AATAAATTCG GTATTAGTTG TCCCGCGTAC 
ACCAAAGACC CGGTCGTAAC ACCGTCAAAA CTGTACTTGG AATATAGCTA CTTACAGTAT 
GAACCCTTCT ACCGACAGAC CTGGTTCATG GTCGCCCTGG CAGCGACCTC CATCATCATT 
ATCATAATGA TAATTGCGAT TTTGTGCGTT AAGAGCAAAA GTTACAAATA TAAACAAGAA 
GGTGCCCAAA ATAGTATGGG CGAACTCATG GCAATGTCAC GCGACGACAT GACCGCTGAC 
TATTACCGAA CTCGCAACGG CACAAGTAAC CTAAGCTCGA CTCTTTCAAA ACGTAGCACT 
TTATCCCGCA AGCCGGTCCA TCAACAACCC CCTCCAATGT TGGGGAAATC CCCTCCGAGA 
CCATCGCCGG CTTCAGTGCC CTACAACTCA GACGAGGAAA GTCTCAAGGG CTACGATGAG 
AATCCCGACG ATAGCAGCGT GACCGAGAAG CCGTCAGAAA TGAGCTCTTC TGAATCCCAG 
GGTTCGGAAA GCGAAAACGA CAGTGTCCTC TCGAATTCGC ACTCGTTTGT GAACCACTAC 
GCCAATGTGA ACGAGCCATT GCGACCGTCC TGGAAGCGCC AAAAGCCGGT CAGGAATTTC 
TCGAGTCACA CTGATTCGGA ACCGGAAGGT TCGGCGAGTT TGAATGGAGG ACAGATTATC 
ATTAATAACA TGGCGAGGTC AAGGGCGCCC CTACCCGGAT TTTCGTCGTT TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100142209 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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