TGME49_264670 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_264670
Entrez Gene ID	7900892
Full Name	DNA polymerase family B protein
Synonyms	TGME49_064670
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018781371.1	XP_018636330.1	DNA polymerase family B protein

Pathways from BioSystems
Gene Ontology

The following TGME49_264670 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_264670 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi10521	XM_018781371.1 Latest version!	Toxoplasma gondii ME49 DNA polymerase family B protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_264670 ( XM_018781371.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018636330.1 Toxoplasma gondii ME49 DNA polymerase family B protein partial mRNA.

Price & Availability↑

CloneID	OTi10521
Clone ID Related Accession (Same CDS sequence)	XM_018781371.1
Accession Version	XM_018781371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	DNA polymerase family B protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031477). COMPLETENESS: incomplete on the 5' end.

ATGGACTCTT TACGAAGAGA AGATGAATGT CTCAGCGCTG CATGGCGCTC AGCCACTCAT 
CGCTGGTCTC TTGGGAGAGG GAGGGGTCAG GCACAGGGGA CAGAACAAAG AGGAAAGAGC 
GCAGACGAAA AACCATGTAC AGGTAGTCCC TCTAATTGTG AGGGATGGAG ACAGACAACA 
GATACACGCG AGGAACGAAC AGACCGGTCA GACTATTTAG GCGAGGGCCG ACTTGAAGAA 
GATGGAGCAG TCGCGAGAGT CTGGAGCGCT CATCCGTCAA GCGTTGACCA CAAGAAAGTC 
GCGTGCTGTT CCCAGCGAGA ATGTGGAGCT TATGTTGGGG GAGAATCTGC GTACAACCCG 
GCCGACATCA GCTGGGGCTC GACACCACAG GAACGCGCCA GAAACGCCCT CAATCCTTCA 
ACTTCTGAAA GCGCTACATA TGAACGAGAG TGGGAGCCAG GGGACTGCCA CCAGGGGCGA 
AGTGACCCTG GAGAACATCC CTCTTCTGCT TCCTCTGCTC GGTCGCATGC CGGAGTCAGG 
CGGGGAGCGA CGGCGAGTCG ACAGCCATCC AGTCGTACAC GAGACGCGTC TGCACTCGAG 
ACTGCGTCGG GATCGGCATC ATCTGATTGT TCGTCTTCCC TGCAACTTCC TTTCTCTTAC 
CGGTCGTCCA AGTCTGCCTC TTCATCTTCT GCTTCCTCGT CTGGTTCTCT CAACGCGTCA 
GCCCCCACAC CAGAAGCCCC GTCGTCGCTG CCTCAGCCGG CGTCTCCTTC CTCGTCCCCT 
CAAGTCTCCT CCATCTGTCC GCAGTCGCCC TTGTCTTCAT CGCCTTTCGC GCAGCTGCAA 
TCTCTGCCAG GTCCGCACTG TCCAAAATCC TCTATGGGTA ACGACAGGCG ATCCTTGGAG 
AACAACAGAG TCCTGAATCC CCTCGTAATT TCGGTCTCAG TGACCGTCGT GGAACCCTGC 
TTGGAGGCTC CAGTGCCAGG GCTTGATCCG ACGACTGCTC CCTTCTCCGG TCGGCGTCTC 
TGGCGTGTTC CTGTTCTTAG AATTTACGGA GTTGTCAATG ATAATCCTGT CAGATCTGTT 
CCTTCCATGT GCCTCTACCC ACCGATGTTA AGAGCGCCAC CAGCGTCCAG TGAATCTCCT 
TTGTCTTCCT CCTCAGCTTC TTCTTTGTCT TCCTCCTCAG CTTCTTCTTC GTCTTCCTCT 
TCGTCTTCTT CTTTGTCTTC CTCCTCAGCT TCTTCTTTGT CTTCCTCTTC GTCTTCTTCT 
TTGTCTTCCT CCTCAGCTTC TTCTTTGTCT TCCTCCTCAG CTTCTTCTTC GTCTTCCTCT 
CTGTCTTCGT CGGTTTCCTA CTCTTCCTCA TCTCCTTCCT CTGCGTCCTC CGCTTGCTCT 
TCCCTTTCGT CACCGAGTTG CGCTTGCCCT TGCCCGCGTG GAGACAGGCG TTTCTTGGGG 
CGCATGTGCT GCGTCACAGT GCACAACGTG TTTCCGTACT TCTTTGTGCC TTTGCCTCGA 
GAGGCACACG AGAAACCAGA GGTTTGGCTT CAGTGGTTTT CTGCAAAACT CCGGGTGTGG 
GAGGACAAGC TGACGAGTCG CAGTGACCCG GGTGCCTCTC GCCCGGCTCG GGGTGGCGGC 
CAGGGACGCG CGAAGCGGAC GGGTCCTGGA CAGGCCCCAG CGCCTTTGTC GACCCCTGGC 
CCACCCCTGG TGTTTGCGTT GCGCCTTGTT AAGAAGCGGG CGTTCTATGG GTACGATCAT 
GCGCCGCGGC TCTTCGTTCA GATTTCCACG CTTCATCCTG GAAGCAAGAC GGTGCAGCTA 
GCTGCACTGT TGCTTCAAGG TCGCGTCACC GGGCATCCGC TTCAGCCCTT TGAGATCCAC 
ATCCCCTTTG TCAGTCACTT TCTCGCCGAC TTTCATCTCC GGGGGATGGA TCTGCTCACC 
GCTGTCGCTC CCTGTTTCGT CCAACCAGTA TTTCTCGCGC CTCCGATGCT TCTGGCTAGT 
CCCGGAGCCC GCGTAGCTTC GGCAGCAGAT CGCACGCATT CCCCCCAGTC TCCCAGCGAA 
GTTCCGGCCT CCGCGAGAGG AGACAGGCAG TCTCTCCGTT GTGCACTGTC TCCTGGGGTC 
TCTGGCTGCC CGGGCTTTTC CTCGGTGCGG AGGCCCGTGG CAGTCGGGGC TCATGAGCGA 
GGACAGGGAC GAGAGGGGCA CACTGCTGAA TCAGGGTCAA ACGACGAGAG GAGGGGGAAC 
GCGTTTTGCA GTCACCACTG TTCCCAGTCT GCGTGGGCCG GTGTGCATCG GGAGGGACAG 
ACGCAGCGGA TTCAAGGTAG GGAATTCATG CCCGCATCTG CAGAATATGT TCCGGGGGGC 
GTGTTTCTCA GCAAATCATG GGCGCAACTG TGGCGCTGCG TGGGGGGATC AAAGACAGCG 
CGAGAGAAGC CATGTGGTGA GTGTGAGCAA CATTTTCGCC ACACAAACGC GTCAGACGAA 
ACGGCTCGAG CCTCTTTGGG GGTTTTCCCC TCTTGTCCCT CTTTGGGCCG ACCGGAAGTG 
CCAGCCAGGG GGCAAGCGCT TCCCGTCATT GCGCCTGCGC TGGTTCCTGA GGAAGGGCTG 
CTTTCTGACG GCAGTCGATT CACCTTTCGT GTGTTGCCGG CGCGCGTGCA GCGGCGAACC 
AAGTGTGAAC TCGAATGCCA TGTGCTGGCG AGAAACATTC TGAATCCGTT TTTCGTGTCA 
AAGGTCGACT CGCCCAACTC CACCGTCTCT CTCAATTGTT CGCTGTCTGC TGAGGAAACA 
AAGGGGACCT CAGCTGCTGA AGCTTGGGAG CGTTCCTCGT CTTTCGCAGC AGACTTACTC 
CTGCCTTCGG TGCTCGACTT CTGGAGAGAG GAGGCTACAC GATGCGCAGG ACTAGGTATC 
TCCTTTCCCT TTGACAGTCC CCACGCGGCA CCCCCAGCCC CCCCGACCCT TCCCTCCGCT 
GCGGCTGCGG CTCTCGCCCA AAGAGACGAG GCACCGGCGC GAGGCGCCGT CCCGGAGATC 
TTCAAGCGAC GTCTCGCTCA ACTCCTAGCA AAAACCGGCG TGCTGACAGA CGTCTCTGCG 
GTGCAGGCCC ACCTGCGTCG AAGCGGGATC GACGAGGCGA CAACGGGTTT GCTGGACCAG 
GAACACGCGT CTCGGCGTAG TGGAACGGCC GCTCTCGACT GCCGTGATCA GAAGGAAATG 
CGAATGAAGA ACTCGAGAGA AACAGGCGAC CTTCCAGGAG ACGCAGGACG TTCACAGGCA 
GGAAGCGAAG GAGAGACAGG AAGGGGACAC CTCGAAGGTC AAGGTGTGGA GAGTTGCGAA 
CAACAGAGTT GGAACCCGAA AAGGCGGGCA GCGTGGGCAG ACTCTGAAGC AGCACGAGGT 
GACGCCTGTG AGAATGTAGA CGCAACTGGA GAGAGCAAAG AAGTAGCTGC ACACAGTGAG 
GTGAACAGAG GCACGCGGCG CGGGGAGAAC GGGGACTCTG GGACGTTACT GGGCTGTGAT 
GCCGACAGGA CAGATTCCGG TGAACAATCT GCCAAGCGTC TGCGCGTCAC TCCGTGTGAT 
GCAGAGGCAT GTGGTGATGG CACCAACGGA GGCCCCCGGC CAGTTCCATC GTTCTCGTCT 
CTCTTCCTTT CACTTCCACA GCAGGAGGCT GAAGATATTC CACGCCCAGG TTCAGCTTCC 
CAATCTTCCG CAGCGTCGTC GTGCCCCCCG CTGTCCCCCG CGAACGCTTC GGGATTTTTC 
ATCGACAGTG CCTTCTCCTT CTCCCCGGTG TCGCAGGTCT CCTCATTCAG CGGGTCTCAA 
GACCCCCTCG GGCCTGACCC CTGCGGGAGC TTCCAGTCCC CATCCGCAGG GGCCTGTCCG 
GTCCCCGGTG TGCCCGCCGG TTCTCCTTGG CAGTGGCTCC ACGCTTCCAG CGCTACGGGA 
GGGGAGTCGA CAAAGGGGGC TGTAGAACGA ACCTCACTAC ACGCGGGAGG GGGCTTTGTG 
GAAGCGCCTT GGAATGCACA ATCCGCTGCT TCCACCAGAA ACCCCCCTCT GGTCGTTTAC 
CCTGAAAGAC CCACCACTGA AATGCTGCTT CCATCAGAAG TACGCATTGC AGGAGATAGG 
CCTGCTCCCT CAATAACTGG AGTGGCACCT TCCCGCTCAA ACGACACTCG ACTAGGAGGT 
CCAAGGTGGC TGGAGGATCC GACGAATGCG CTCTGTCACT TAGGCAGTCG AACGGATTCT 
GCTTCTCAAC GGAATCCATC AGAGACGCGA GAGGCACCTG TAGTCCAGTC ACCGGCCGCG 
CCTGCGCCTT CGCTTGCGCC TTCACACTCA GAGGTCTGGA GAGAAAGTGG GGTTGTTCAC 
TCTGCCACGG CACTTCCTTT GCCTCCAGTC GAACACCCCA AAGCGAGCGG ACGTGATCCT 
TTTGAGGAGC GTTTGCCTTC GCAGGATGCG CCCCTGGAGA GCTCTTACTC GGCGCCGTGG 
CGTCAGATGC CGAGCTCAGG GCACGTAGGT AGCTTCGGGG ATAAAGAGAT AAGAAGGTCG 
TGGCCGGAAT GGTCGGGGGA CAAAGTGTTT CTCGAGCTGA ACGGAGTCAA TTCAACGGAA 
GCGATCCTGA AGTCTTCGGA GCATGAGGTG CGCGAGGCGA AAGAAGGAGA AGTCGTGGAG 
CTCGCTGAAG GATGGAGAGA TCAAGACGAC GACGAGAGTG GCGGCAAGGA AGAAACGGTG 
TGTGGCGTGA GAGCTGCACA ACCGGGGAGA CTCGCGATAG AAGACATAGT TACTGTAGAA 
GCCGAGATCA AAGGAAAAGG CGCCCTAGAG GAGGAGGCAG ACTGGGCCAT CGCCGCTGTG 
CCTCACGATC GCAGTGAGGA AGAGGTACCC TCTTCAGCTG CAGAAGCGGG AGGAACAAGG 
TGCGTGCCCC TCGGGGGCGG CGTCACAGCT CTAGAGCCTT TCGAGCTGTT TGCGCCACAG 
GTCTGCAGCC GATCTCAGTC AAGTTCATCG GGGAGCAGTA GCTCAGTAGA AGGGCGCCGC 
GCGCGGAGAA GAAGAAACAG GCTACTTAGA AGCGGACAGA GCCGAAAAAC GTCTTCGCTG 
GACAGCAGCA GTTGCTCGCC GACGGTCGAC TACGACGCGA GTGCGAGAAG CAGCGACGAC 
AGCGTGTCGA GCGACCCCTG CGGGGCTCTG GACATGGAAG TCGACTCCAC GAAAAATGGG 
AAGCCAAAGG AGGGGAATGC AGAGGAATCG GATACATCTC GAGTACGCGG GGAAGAGGGC 
CAAGCCACAT TTCCTACCAA CAAGTCAGGG GAAGCAGCGA GCAAAAAGCC GTCTGCAGGA 
TTGGCGTCCG GGTTCCAGGA CCGTCAAGAA GTCTGCAGGT CACGAGCCTC TGACTTTCTA 
CACCGCAGGT GTGTTTTTTC TGGCTCCGAA GAGAAGACGG CGTGTCTCTC CACAGCCGTC 
GACATGCATA TGCCTATGTC GAATCTTTCA CTCGACGCGA AAGTAACTCT CGGCCGTGTC 
CAGACGCCAA CTGGGACGTC TACAGATGTG CGGCACGCGA CGGGGATCCA CAGCACCGGG 
AAGATGGAAG ACAACCTGCG CGAGGCGGAC CTGGTCTCGA AGCGCTTGTC GCAGGGGTCG 
GCATGCGAGG ACCATTCAGG AGATGAAGAC CGTGGAACTT CGTCATCGTA TGCGGCCACG 
GTCGTTCTCT CCAGTGGTTC GGCCGAAGGA CGAAAGGAGC GCTTTGGCGG TGAAGCAACC 
GAATCAAGAA ACAGGCAAGA GGCGCCTCCA CAGCGGGCAA AAGAAAAAGA AGAGGAGACG 
GCTTCACCAC CTACCCCTGA CCGCACACGC GAGAACACTG AAAAGCATGT GGGCATGCGT 
AGCCGCCACT CCTCTCACAC ATCGAAAGAA AAATGGGAAG GACTGGAGCA CGCTCGCTTA 
CAGAATCTGT CGGAACATGG CGACTCATCT CAGGGCCCCG TCTTGACCTT GTTATTTCCC 
CGGGGTGAGC CGAAGTCGCC TCAGGTCGTC GCCGTAAACC CCACATCCCA TGCTCTCTTG 
GATCGGCACT GTTCTGTATC CCAGCAGTCG CTGCAGGGCT GGCCAGATGA GCCCGCAGAG 
CTCCCTTTCT TTGGCGACCC TCGCGATTTG CCGGTGAGCG TCCTCCGCGC CGTTGAAGCT 
ACTCTGGGAG GGCGCCAGTT GCGTGAACAG GAGGAAACAG TTCTTGGAGC CCGTCGCGCA 
GAAAGCATCG CAGCAGCAGC AGCGGAGGAG ACTCGCCTTC TGGCCGAGCG GCGTGCCAAG 
CTAGGCAGGA AGACGAGGAG ACGCCGACGG AAGAGAAAGA ACGCTCGACA GTCGCAAGTC 
TCCTCTTCTT CGCAGCAAAC GGCTTCTCAG TCGTCGCAGT GCGCCTCTCA AGAGCCATCT 
CAGCGTGGCT CGCAGAGCTC TGGGCGCTTC GGCGTGCTTG TTGACTATCA TTGCAAACAA 
CGCCCGCATG TAGAGCGGCG TCGGAGCGGT GCTGTCTCCA CAGATGCCTT AAGCAAGCAA 
GCGCCAGGTC GAGAGGCAGG GACCACAATG GAGCCCGAAC CGGGAAGAAG CGGTTGGTTT 
GGTAGTGACG CGGGCCGCGT ACAACCGTCG CTTGTTTTGC CAGGAAAAGC TGTCCAGCCG 
GACACACTTG TTGTGGAGAT TTCGTCGTCC TCAGACAGTG GTAGCGATGG GGTCTGCAGC 
CGCTCTGCGG TTGTGCTGAC GACGTGGGAT GCGACGGGCA GAGGGCCAGG AGAGACGAGT 
TTGCCTTCCG CTCCTGTCGT GGCTATCGCC GACCGAACAA ACGGGGAGAC CTTCCCAGGG 
ACCGCGTTCC CACACGGTTC TACCAAGCCT CCAGGAAACG CTTTCTGGGG CTTCTATAAG 
CCGCCCCCCA CGACCCACGA GGCGCTCGCG AGTTGCCCCC CCAGGGTGCA GAAGAACCAT 
GAGCGGCTGT TGCGTCGGGA GGCACGCAGA GAGGCAAGGC GCCGACAGCG GCGCCAGAGC 
TGGGGGGGTG AGGCCGAGCG AGCGCGAACC GGCGACAGTT CTGCCGCGTC GCGGTGCGAA 
CGAGTGCGAG AACCTAAGGG CGACAGTAAG CGCGATTTTA AACGACAGAA CCGAAGAAGC 
AGAAGGGACT ATAGTCCGAA CAGGAAGGGA CGCGAGTGCA GAAAAGAGGT GAGGCCGGCG 
TTGAGATGGG CCACAAGGAT GGATAATGGC GGGCGTATCG TCCTGATGCT AGTGCCAGCA 
TCGCAGTCCA GCGGAGTGGA ACGAGAATCG GCACGACGAG AAAGAAGAAG TCGCACTCGA 
AGAACGATAT CGCTTTCTCG ATCCCGAGCA GAACCGACGG TGACGCGACC CTCGGAAATA 
CCGCCGGCTT CCTCATCTGC GTCTGGCTGC TGCACCTCTG AGGGCTTTAG CCATACTGGA 
GATGACGCAG CCGCCGATAG GAACTGGACC GACGAAACGA GGGCGCGTGC GGATCTTCCG 
AGTCGAGGGT TGCCGTTTGC GACTGCATGC GTGGAAGCTG AGAGCTGCCC TCAAGACGTG 
GGAAAGCCGC GAAATCCACG GAAAACAGCA GGTGTCGATG AGGTGAAGGT GGACCACGAT 
CCACCGACGT CGCCGAGCGC AAATGAACTG AAGGGGAAAC GCAGCGACTC CGATGATGAG 
GGGGAAGGTG GCGAACGCTT GGGTGCTCCT GAAAGAGCCC GACGCAACGA GGCGAACGAC 
TTGCTACCGC ATCAAGATGT TGAAGTGAAT TCTGTTCAGC CGCGCGATAG GCATATCTGT 
GACGGGACCT CTCCTGTCTG CGACAGGGAG CATGTACCTT CTTCCCAGAC ACAGCAATGT 
GGTTCGTTGT GCCTGTCAGC CGGCAGTTTG ACGACAGGCC ACTCGTTCTC AAGCGCGTGC 
TCCAGTAGCG ACGTCTCCCT GGGAGGGGTG GGAGAAGGCG CCAGGAATGT TTCGCAGCTC 
AGTGTCGCAT CAGCTCTTAA AGACGCGGCA AAACAGGCTC GGCCGTTTCA TGCCTCCGCG 
GCAAACGAGC TTGCCGACGA GGCGATTCGT GGGGAAAGGA CACTCGCCAG CTACGGCGCG 
TTGTTGGCCG TCGAAGTCCT CGCAGAGAGG ACACAATGCA GCCCTGAAGC TGTCGATGCG 
GGTGCGCCCG ATCCTCAGCA GGACGCCATC CTGGCTGTCG CTTTGGTGCT CAGAGACGAG 
CGTCTGTCTT TGGCTTGCCG TCGGGCGCAG CGGGCGAATG GGAGACACGG GGGAGACGCG 
GAAAGGTGGG ACAAGGAAGC AGAGGGGCCA GAAGACCAGG GCAAGGAGGG CTTGGGCAGA 
AGAGAGGGAA GAGAAACGGA CATGGAAATG GGAGCAAAAG GGAACAGGAA CGGAGACAGT 
CAAGAGGCGG AAAGCAGCTG CGGGGACCCA CTAGCGAACT CGGGTTCCGA GACGCAGAGA 
CGGCGGAAGA GCGGGAGCAA GCCGTTTCTG TCCACGGCCC TAGGTCGGGA GCGATGCGCA 
GAAAAGGCGG CGGCAAGTCT CGCCTATTTC GATGCTACCA TGATCATTTT TGTTGACCAG 
GCCTACAAGC CTCGCCTGCC CTTGAACTCC AGATTATGTG CTCGCTCTCC CGGATGCCGT 
TCTGCCTCTG TTCCGTCGTC ATCAACTGCT CACTCCCTTA CCAGCTCCTC GGTCGCCTCG 
CCGTTCACGC GAGCTCGCCA GTCGTTCGCG GACTTTGTCG CTCTATGGCA GTCTCGTCAT 
CCGTTCACCA AGTCTGCCCA GCAGCAACTG CCCGCCTTCC TTCCGTCTGA TACTGAAGTG 
GTTGCCGTAG AAAGCGAAAG AGCCCTCATT CATTATGTTT GTCTTATCTT TAGTGTTGCC 
GATCCTTCTA TGGTTCTGCA GTGGGACAGT GGATGCATGG GCCTCGGCTT TCTTCTCCGC 
AGGGCGCGAG TTCTCGGCTT TGGTGCAGAA CTCACAACAG CTCTCGGGAG ACGAGCAGAT 
CCTCTCCTTC TCTCACATCT CCGATCTATC TCGTATCGAC CCAGGTCGCA CGTGGCCATC 
TCTGTGTCTC CAGCGCCTGT TTCGCAGCAG TCTTTTGGCT CTGTTTCATC TTCCTCTCCA 
GCCCTGCCGC AGGCTGAATC AACCTCAGCT AGCTCGTGGT CTCGCCCGTC TATAACGGAT 
CACCGTGAGG CACGACACCC CACACCGCGA GAGTCAGGGA GGTCAAGTGG GGTCTCCGAC 
GTGCTCCTCC AAGGAGGGAT GCTGTCGGGC CGGCTTCTTC TCGAATGTTG GCGGCTGCTT 
CAGAGTGAAG TGAAACTCCA GCAGTCGTCT CTGGAAGGAG TTGCCACAGA GGTCCTTGGT 
GTGACCTTCG CCTCGGTGCC TCCGGCGATT CTTGCGAACC TCTGGAAGCA GGGACAGGGC 
GCTTTGAGGC GCGCAGAGAG GCTTGACGAA GTTGAACTCA TGACAGCGTG GCGTGAATGG 
CAGTTGCGCG TGAGGAGACA CGAAGAGGTA CAGGGTAGGC CAAGAGACAC AGCTGAGGGG 
GGTAAGGGCC ATGTTTTTCA TGGAGAGACG CAGGGTCCAC GAACAGAGGG TCCACAGACA 
GCTGATGGGG CTGGGCTACA GCACATCGAT GCAGCACAGA GAACCCTGGG CGAGCCAGAC 
AGTCTGGTCC TGGAGCAGAT CTTTGCAGAC GTTGAGCGAG CAACAGGCAA TCCGATGGGG 
CGGCTGCCGG GGCCTGAACC AAGAGAACGG GACCAAGAAG TAGTGTGGGC CGTTTCGACT 
ATGGGTGCGG TCCTCAAGTA CCTTCTGGGC AGGCCCCAGC GGCGATGTGC CCTGACGAGA 
GGGAGTCAAT TCAAGGTGGA AAGCGTGATG ATCCAAGCAA CCAAGCGCCT TGATTATCTG 
TTTGTGTCTC CTTCTCAAAC TCAGGTGACA GAGCAGCCGG CACCGCTGTG TATTCCACTC 
GTTCTCGAAC CCAAAAGTGG GTTTTACTGG AGTCCCGTAC TGGTTCTAGA CTTCCGTTCT 
CTCTACCCGT CCATCGTGAT CGCCAACAAC ATCTGCTACT CGACGGCTCT TGGCTCCGTT 
GAGATCGATC CGACAGTGCA AGCCACGAAG ACGTTGGGCG TGATGGACTT CCACTGTCCT 
CCGGCTCTGT TCCGGCAGAT TTCGGAAGCC TACTACCGAG CTGCTTCCGT GGACCTAAGG 
AAGGGAGCAC CGGCTCTTCC TGACCCTGTT GGGGGTGCTG GTGAGCGGAA GGGCGGAAGG 
GATAGTGGCG AACGACGAGG CCACAGCCAA GCTTCGAGCT TTTGTGCAAG CCAGGCGTGG 
GACGCAGACA GTGGTGGACT TCGGCTTTTG CCAAGCGGGG CCATGTTCGT GTCGCGCCGT 
GTAAGGAAGG GGATTTTCCC TCAAGTCCTC AGCGAGATCC TGCAGACCCG CATCATGGTC 
CAAAAAGCGA TCAAAAAATA CAAAGGGAAA GTGGAAGAAG GCTTGCTCCG ACTCCTAAAC 
TACAGGCAGT ATGGCCTAAA AATGATTGCT AACGTTTCCT ACGGTTATAC AGCAGCAAGT 
TTTACGGGGC ACATGCCGTG CGCCGATATT GCCGATGCAA TCGTACAGAC AGCCCGGACA 
ACGCTCATGC GCGCCATCGA ACTGGTCCAT AACACTCCAC GATGGGGCGG AAGAGTATTG 
TACGGAGACA CGGACAGCAT GTTTGTTCTG GTCGAGAACA AGTCCCTGGA AGCTGCCTTT 
GAGATTGGAC GTGAAATCGC GTACACTGTC ACTCAGCAGA ATCCGTCACC AGTTGAGCTG 
GAACTTGAAA AAGTTTTCTA CCCCTGCTGC CTCGTCTCGA AGAAGCGCTA CGTGGGGAAC 
GCGTATGCAG CGCCGAACCT GCCTCCAACC TTTGAAGCGA AGGTCAAGGC GCATCTCTAC 
CGGCAGTGGA CAAAGATTAT GTTAAACCAG GTCCCCTTGA AGGATTTCAT TTTCTACAAG 
AAATGCAGGC TCGGTTCCTA CAAAGCCGAG TTTGGCGCAT CAACTGCTGC ATCCCTACCC 
CCGCAAGCTA AAGTGGCATA TGCACGGTTG CAAGCGCTTG TTCAATCCTC TGTGTGCTTG 
CAAGATGTGC TTGGCACCGG CACGTCGGAA ACTGCTCCTG CGTTGCCGTC AGGACCATCT 
CGCGGAGAGC GAGTGGCATT TGTCTACGCG GATCTCGGCC TCGGAGGAGC AGAAGCGTTT 
TTGACAGGAA AGCAGAGCGC GTTGCGGCTG CTTGATTGTG CCGTGAGCCC TGAACAAGTC 
GAAGGGGGAG GGAGGGCGGA TAACCTTGTC AGCCTCTTCC TCTACGGTGC ACCTCAAAGG 
GTGCTGCGCC CCATGCCACT CCAGCTTCCA GACGCTTGGA CAACTGGATC GGTGACGCCA 
CCGCAAGAGG GGACCGCCAG GGACTTTTGG AGTCAGCACG CTGGCTCTTG TGTCTGGCTT 
CGATGTTGGG AGCCCATGCC ACGTTCAACA GTAGTAAAGG GTACAGACTC ATCATTGTTT 
CCTGAAGGTG AGAGAACACA AGCGGCGTCC CCAGCGTTGC AGGCGCCCGC CTCCCGCTTC 
GGCTGGCGTC GCCTTGGCAT GAGTCCAGCT GGTGCGTCCT GCTCTGGTTC GTTCTCTGTA 
TCGGTGCAGC AGGGCCAAGA GTGTAGTGGT ATTGCACCAT CTGCTGTGCA AAGCCTTGGA 
GAGGGTAATT CCGAGCAACT GCTGTTGCCA GTATATCTGA AATACTACAT CGTACGGCAA 
GTCATTCCTG CCCTCGACCG CCTCTTCGGT CTTCTTCCCG GGTCAACATC TGTAGATCTT 
CGGCAGTGGT TCGACAGAAT GCCGAAGCCT CAAACAAAGC CAGCGACGAG ATTGCGTCGT 
CAGCCACAAA CCGGCCAACA GGTAGTGAAG AAACCTAGAC AGGGCATGCT GACGTTTGGG 
ACAAGACCGC TTCCACGGAA AATAAGTCCG GAAAGCCGGT TACTACGAGC TCGACATGGG 
TCGGTGCCTC AGAGGACTCC AGTGGTAGAT CAGATGGCAG TTCCGTCGGA TGCTGGTGCG 
GTGTCGCCGG CGCCTCAGCC CCGGCACAAA TTGCTGCAGG CTTTGAAAGT AAAAAACGGG 
GCCGGTGGCG GAAGCAGAGT ACGCCTTCAC CACTTTTTTG CGGCTTCCAC CTGCCTGTTC 
TGTGGTGAGA GGTGCTCTCT GTTACCTCCT GGATCTCTCC GAGAGCTGCT GGGCTCCAGC 
GCCAGAGCAA ATGGGAGTAA AGGGAGTAGT GGAGGTCACA AAGGAGACGG GAGCACAGAC 
AAGGTGAGGC GAAGGCGCGG AGGAGGTGCA ATTCATTTTG AAGAGGACAC AGAAGCAGCT 
GTCAGAGCAC AGCTTTTCAG AAAATTTGAG TGTCGTAACC TCACCAGTGA CGAAGATGGA 
GAAGGTAACG AAAATGGGGG ACGCAATAAC GATGGAAAAT TAGTCATCGA CCTTTCGTCC 
AAGTCTGCTT TCTCTTCAAC ATCTCATGGG TCAGTCTTCT GGCCTTTGTT TCAGTCCGCG 
TCAGCCCGAG AGAAGCAACG CTACTGGCAG CCTCCTCCGG TGTGTGCAGC GTGTAGCAGG 
GATGCCGAAC GCCTTCTTGT GGTAGCATAT TCGGAGTTGC GCGAGGAAGA GAAAAGGGTA 
AAAGAGGTTG AAGACATCTG CGAGGCATGC GCAGGGAGCA GACTCTGTGC AGCGGCATGT 
TTGAACGCTT GGCACTGTGA CGTATATTTT AGAAAGCTGC GAAACGACAT GCGCCTAGCA 
GCTGTCTCCT CCAAATTACG CGCACTTCAT TTGCACCTGA AGGATAGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018636330.1
CDS	1..12774
Translation	MDSLRREDECLSAAWRSATHRWSLGRGRGQAQGTEQRGKSADEKPCTGSPSNCEGWRQTTDTREERTDRSDYLGEGRLEEDGAVARVWSAHPSSVDHKKVACCSQRECGAYVGGESAYNPADISWGSTPQERARNALNPSTSESATYEREWEPGDCHQGRSDPGEHPSSASSARSHAGVRRGATASRQPSSRTRDASALETASGSASSDCSSSLQLPFSYRSSKSASSSSASSSGSLNASAPTPEAPSSLPQPASPSSSPQVSSICPQSPLSSSPFAQLQSLPGPHCPKSSMGNDRRSLENNRVLNPLVISVSVTVVEPCLEAPVPGLDPTTAPFSGRRLWRVPVLRIYGVVNDNPVRSVPSMCLYPPMLRAPPASSESPLSSSSASSLSSSSASSSSSSSSSSLSSSSASSLSSSSSSSLSSSSASSLSSSSASSSSSSLSSSVSYSSSSPSSASSACSSLSSPSCACPCPRGDRRFLGRMCCVTVHNVFPYFFVPLPREAHEKPEVWLQWFSAKLRVWEDKLTSRSDPGASRPARGGGQGRAKRTGPGQAPAPLSTPGPPLVFALRLVKKRAFYGYDHAPRLFVQISTLHPGSKTVQLAALLLQGRVTGHPLQPFEIHIPFVSHFLADFHLRGMDLLTAVAPCFVQPVFLAPPMLLASPGARVASAADRTHSPQSPSEVPASARGDRQSLRCALSPGVSGCPGFSSVRRPVAVGAHERGQGREGHTAESGSNDERRGNAFCSHHCSQSAWAGVHREGQTQRIQGREFMPASAEYVPGGVFLSKSWAQLWRCVGGSKTAREKPCGECEQHFRHTNASDETARASLGVFPSCPSLGRPEVPARGQALPVIAPALVPEEGLLSDGSRFTFRVLPARVQRRTKCELECHVLARNILNPFFVSKVDSPNSTVSLNCSLSAEETKGTSAAEAWERSSSFAADLLLPSVLDFWREEATRCAGLGISFPFDSPHAAPPAPPTLPSAAAAALAQRDEAPARGAVPEIFKRRLAQLLAKTGVLTDVSAVQAHLRRSGIDEATTGLLDQEHASRRSGTAALDCRDQKEMRMKNSRETGDLPGDAGRSQAGSEGETGRGHLEGQGVESCEQQSWNPKRRAAWADSEAARGDACENVDATGESKEVAAHSEVNRGTRRGENGDSGTLLGCDADRTDSGEQSAKRLRVTPCDAEACGDGTNGGPRPVPSFSSLFLSLPQQEAEDIPRPGSASQSSAASSCPPLSPANASGFFIDSAFSFSPVSQVSSFSGSQDPLGPDPCGSFQSPSAGACPVPGVPAGSPWQWLHASSATGGESTKGAVERTSLHAGGGFVEAPWNAQSAASTRNPPLVVYPERPTTEMLLPSEVRIAGDRPAPSITGVAPSRSNDTRLGGPRWLEDPTNALCHLGSRTDSASQRNPSETREAPVVQSPAAPAPSLAPSHSEVWRESGVVHSATALPLPPVEHPKASGRDPFEERLPSQDAPLESSYSAPWRQMPSSGHVGSFGDKEIRRSWPEWSGDKVFLELNGVNSTEAILKSSEHEVREAKEGEVVELAEGWRDQDDDESGGKEETVCGVRAAQPGRLAIEDIVTVEAEIKGKGALEEEADWAIAAVPHDRSEEEVPSSAAEAGGTRCVPLGGGVTALEPFELFAPQVCSRSQSSSSGSSSSVEGRRARRRRNRLLRSGQSRKTSSLDSSSCSPTVDYDASARSSDDSVSSDPCGALDMEVDSTKNGKPKEGNAEESDTSRVRGEEGQATFPTNKSGEAASKKPSAGLASGFQDRQEVCRSRASDFLHRRCVFSGSEEKTACLSTAVDMHMPMSNLSLDAKVTLGRVQTPTGTSTDVRHATGIHSTGKMEDNLREADLVSKRLSQGSACEDHSGDEDRGTSSSYAATVVLSSGSAEGRKERFGGEATESRNRQEAPPQRAKEKEEETASPPTPDRTRENTEKHVGMRSRHSSHTSKEKWEGLEHARLQNLSEHGDSSQGPVLTLLFPRGEPKSPQVVAVNPTSHALLDRHCSVSQQSLQGWPDEPAELPFFGDPRDLPVSVLRAVEATLGGRQLREQEETVLGARRAESIAAAAAEETRLLAERRAKLGRKTRRRRRKRKNARQSQVSSSSQQTASQSSQCASQEPSQRGSQSSGRFGVLVDYHCKQRPHVERRRSGAVSTDALSKQAPGREAGTTMEPEPGRSGWFGSDAGRVQPSLVLPGKAVQPDTLVVEISSSSDSGSDGVCSRSAVVLTTWDATGRGPGETSLPSAPVVAIADRTNGETFPGTAFPHGSTKPPGNAFWGFYKPPPTTHEALASCPPRVQKNHERLLRREARREARRRQRRQSWGGEAERARTGDSSAASRCERVREPKGDSKRDFKRQNRRSRRDYSPNRKGRECRKEVRPALRWATRMDNGGRIVLMLVPASQSSGVERESARRERRSRTRRTISLSRSRAEPTVTRPSEIPPASSSASGCCTSEGFSHTGDDAAADRNWTDETRARADLPSRGLPFATACVEAESCPQDVGKPRNPRKTAGVDEVKVDHDPPTSPSANELKGKRSDSDDEGEGGERLGAPERARRNEANDLLPHQDVEVNSVQPRDRHICDGTSPVCDREHVPSSQTQQCGSLCLSAGSLTTGHSFSSACSSSDVSLGGVGEGARNVSQLSVASALKDAAKQARPFHASAANELADEAIRGERTLASYGALLAVEVLAERTQCSPEAVDAGAPDPQQDAILAVALVLRDERLSLACRRAQRANGRHGGDAERWDKEAEGPEDQGKEGLGRREGRETDMEMGAKGNRNGDSQEAESSCGDPLANSGSETQRRRKSGSKPFLSTALGRERCAEKAAASLAYFDATMIIFVDQAYKPRLPLNSRLCARSPGCRSASVPSSSTAHSLTSSSVASPFTRARQSFADFVALWQSRHPFTKSAQQQLPAFLPSDTEVVAVESERALIHYVCLIFSVADPSMVLQWDSGCMGLGFLLRRARVLGFGAELTTALGRRADPLLLSHLRSISYRPRSHVAISVSPAPVSQQSFGSVSSSSPALPQAESTSASSWSRPSITDHREARHPTPRESGRSSGVSDVLLQGGMLSGRLLLECWRLLQSEVKLQQSSLEGVATEVLGVTFASVPPAILANLWKQGQGALRRAERLDEVELMTAWREWQLRVRRHEEVQGRPRDTAEGGKGHVFHGETQGPRTEGPQTADGAGLQHIDAAQRTLGEPDSLVLEQIFADVERATGNPMGRLPGPEPRERDQEVVWAVSTMGAVLKYLLGRPQRRCALTRGSQFKVESVMIQATKRLDYLFVSPSQTQVTEQPAPLCIPLVLEPKSGFYWSPVLVLDFRSLYPSIVIANNICYSTALGSVEIDPTVQATKTLGVMDFHCPPALFRQISEAYYRAASVDLRKGAPALPDPVGGAGERKGGRDSGERRGHSQASSFCASQAWDADSGGLRLLPSGAMFVSRRVRKGIFPQVLSEILQTRIMVQKAIKKYKGKVEEGLLRLLNYRQYGLKMIANVSYGYTAASFTGHMPCADIADAIVQTARTTLMRAIELVHNTPRWGGRVLYGDTDSMFVLVENKSLEAAFEIGREIAYTVTQQNPSPVELELEKVFYPCCLVSKKRYVGNAYAAPNLPPTFEAKVKAHLYRQWTKIMLNQVPLKDFIFYKKCRLGSYKAEFGASTAASLPPQAKVAYARLQALVQSSVCLQDVLGTGTSETAPALPSGPSRGERVAFVYADLGLGGAEAFLTGKQSALRLLDCAVSPEQVEGGGRADNLVSLFLYGAPQRVLRPMPLQLPDAWTTGSVTPPQEGTARDFWSQHAGSCVWLRCWEPMPRSTVVKGTDSSLFPEGERTQAASPALQAPASRFGWRRLGMSPAGASCSGSFSVSVQQGQECSGIAPSAVQSLGEGNSEQLLLPVYLKYYIVRQVIPALDRLFGLLPGSTSVDLRQWFDRMPKPQTKPATRLRRQPQTGQQVVKKPRQGMLTFGTRPLPRKISPESRLLRARHGSVPQRTPVVDQMAVPSDAGAVSPAPQPRHKLLQALKVKNGAGGGSRVRLHHFFAASTCLFCGERCSLLPPGSLRELLGSSARANGSKGSSGGHKGDGSTDKVRRRRGGGAIHFEEDTEAAVRAQLFRKFECRNLTSDEDGEGNENGGRNNDGKLVIDLSSKSAFSSTSHGSVFWPLFQSASAREKQRYWQPPPVCAACSRDAERLLVVAYSELREEEKRVKEVEDICEACAGSRLCAAACLNAWHCDVYFRKLRNDMRLAAVSSKLRALHLHLKDSH

Target ORF information:

RefSeq Version	XM_018781371.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 DNA polymerase family B protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018781371.1

ORF Insert Sequence:

ATGGACTCTT TACGAAGAGA AGATGAATGT CTCAGCGCTG CATGGCGCTC AGCCACTCAT 
CGCTGGTCTC TTGGGAGAGG GAGGGGTCAG GCACAGGGGA CAGAACAAAG AGGAAAGAGC 
GCAGACGAAA AACCATGTAC AGGTAGTCCC TCTAATTGTG AGGGATGGAG ACAGACAACA 
GATACACGCG AGGAACGAAC AGACCGGTCA GACTATTTAG GCGAGGGCCG ACTTGAAGAA 
GATGGAGCAG TCGCGAGAGT CTGGAGCGCT CATCCGTCAA GCGTTGACCA CAAGAAAGTC 
GCGTGCTGTT CCCAGCGAGA ATGTGGAGCT TATGTTGGGG GAGAATCTGC GTACAACCCG 
GCCGACATCA GCTGGGGCTC GACACCACAG GAACGCGCCA GAAACGCCCT CAATCCTTCA 
ACTTCTGAAA GCGCTACATA TGAACGAGAG TGGGAGCCAG GGGACTGCCA CCAGGGGCGA 
AGTGACCCTG GAGAACATCC CTCTTCTGCT TCCTCTGCTC GGTCGCATGC CGGAGTCAGG 
CGGGGAGCGA CGGCGAGTCG ACAGCCATCC AGTCGTACAC GAGACGCGTC TGCACTCGAG 
ACTGCGTCGG GATCGGCATC ATCTGATTGT TCGTCTTCCC TGCAACTTCC TTTCTCTTAC 
CGGTCGTCCA AGTCTGCCTC TTCATCTTCT GCTTCCTCGT CTGGTTCTCT CAACGCGTCA 
GCCCCCACAC CAGAAGCCCC GTCGTCGCTG CCTCAGCCGG CGTCTCCTTC CTCGTCCCCT 
CAAGTCTCCT CCATCTGTCC GCAGTCGCCC TTGTCTTCAT CGCCTTTCGC GCAGCTGCAA 
TCTCTGCCAG GTCCGCACTG TCCAAAATCC TCTATGGGTA ACGACAGGCG ATCCTTGGAG 
AACAACAGAG TCCTGAATCC CCTCGTAATT TCGGTCTCAG TGACCGTCGT GGAACCCTGC 
TTGGAGGCTC CAGTGCCAGG GCTTGATCCG ACGACTGCTC CCTTCTCCGG TCGGCGTCTC 
TGGCGTGTTC CTGTTCTTAG AATTTACGGA GTTGTCAATG ATAATCCTGT CAGATCTGTT 
CCTTCCATGT GCCTCTACCC ACCGATGTTA AGAGCGCCAC CAGCGTCCAG TGAATCTCCT 
TTGTCTTCCT CCTCAGCTTC TTCTTTGTCT TCCTCCTCAG CTTCTTCTTC GTCTTCCTCT 
TCGTCTTCTT CTTTGTCTTC CTCCTCAGCT TCTTCTTTGT CTTCCTCTTC GTCTTCTTCT 
TTGTCTTCCT CCTCAGCTTC TTCTTTGTCT TCCTCCTCAG CTTCTTCTTC GTCTTCCTCT 
CTGTCTTCGT CGGTTTCCTA CTCTTCCTCA TCTCCTTCCT CTGCGTCCTC CGCTTGCTCT 
TCCCTTTCGT CACCGAGTTG CGCTTGCCCT TGCCCGCGTG GAGACAGGCG TTTCTTGGGG 
CGCATGTGCT GCGTCACAGT GCACAACGTG TTTCCGTACT TCTTTGTGCC TTTGCCTCGA 
GAGGCACACG AGAAACCAGA GGTTTGGCTT CAGTGGTTTT CTGCAAAACT CCGGGTGTGG 
GAGGACAAGC TGACGAGTCG CAGTGACCCG GGTGCCTCTC GCCCGGCTCG GGGTGGCGGC 
CAGGGACGCG CGAAGCGGAC GGGTCCTGGA CAGGCCCCAG CGCCTTTGTC GACCCCTGGC 
CCACCCCTGG TGTTTGCGTT GCGCCTTGTT AAGAAGCGGG CGTTCTATGG GTACGATCAT 
GCGCCGCGGC TCTTCGTTCA GATTTCCACG CTTCATCCTG GAAGCAAGAC GGTGCAGCTA 
GCTGCACTGT TGCTTCAAGG TCGCGTCACC GGGCATCCGC TTCAGCCCTT TGAGATCCAC 
ATCCCCTTTG TCAGTCACTT TCTCGCCGAC TTTCATCTCC GGGGGATGGA TCTGCTCACC 
GCTGTCGCTC CCTGTTTCGT CCAACCAGTA TTTCTCGCGC CTCCGATGCT TCTGGCTAGT 
CCCGGAGCCC GCGTAGCTTC GGCAGCAGAT CGCACGCATT CCCCCCAGTC TCCCAGCGAA 
GTTCCGGCCT CCGCGAGAGG AGACAGGCAG TCTCTCCGTT GTGCACTGTC TCCTGGGGTC 
TCTGGCTGCC CGGGCTTTTC CTCGGTGCGG AGGCCCGTGG CAGTCGGGGC TCATGAGCGA 
GGACAGGGAC GAGAGGGGCA CACTGCTGAA TCAGGGTCAA ACGACGAGAG GAGGGGGAAC 
GCGTTTTGCA GTCACCACTG TTCCCAGTCT GCGTGGGCCG GTGTGCATCG GGAGGGACAG 
ACGCAGCGGA TTCAAGGTAG GGAATTCATG CCCGCATCTG CAGAATATGT TCCGGGGGGC 
GTGTTTCTCA GCAAATCATG GGCGCAACTG TGGCGCTGCG TGGGGGGATC AAAGACAGCG 
CGAGAGAAGC CATGTGGTGA GTGTGAGCAA CATTTTCGCC ACACAAACGC GTCAGACGAA 
ACGGCTCGAG CCTCTTTGGG GGTTTTCCCC TCTTGTCCCT CTTTGGGCCG ACCGGAAGTG 
CCAGCCAGGG GGCAAGCGCT TCCCGTCATT GCGCCTGCGC TGGTTCCTGA GGAAGGGCTG 
CTTTCTGACG GCAGTCGATT CACCTTTCGT GTGTTGCCGG CGCGCGTGCA GCGGCGAACC 
AAGTGTGAAC TCGAATGCCA TGTGCTGGCG AGAAACATTC TGAATCCGTT TTTCGTGTCA 
AAGGTCGACT CGCCCAACTC CACCGTCTCT CTCAATTGTT CGCTGTCTGC TGAGGAAACA 
AAGGGGACCT CAGCTGCTGA AGCTTGGGAG CGTTCCTCGT CTTTCGCAGC AGACTTACTC 
CTGCCTTCGG TGCTCGACTT CTGGAGAGAG GAGGCTACAC GATGCGCAGG ACTAGGTATC 
TCCTTTCCCT TTGACAGTCC CCACGCGGCA CCCCCAGCCC CCCCGACCCT TCCCTCCGCT 
GCGGCTGCGG CTCTCGCCCA AAGAGACGAG GCACCGGCGC GAGGCGCCGT CCCGGAGATC 
TTCAAGCGAC GTCTCGCTCA ACTCCTAGCA AAAACCGGCG TGCTGACAGA CGTCTCTGCG 
GTGCAGGCCC ACCTGCGTCG AAGCGGGATC GACGAGGCGA CAACGGGTTT GCTGGACCAG 
GAACACGCGT CTCGGCGTAG TGGAACGGCC GCTCTCGACT GCCGTGATCA GAAGGAAATG 
CGAATGAAGA ACTCGAGAGA AACAGGCGAC CTTCCAGGAG ACGCAGGACG TTCACAGGCA 
GGAAGCGAAG GAGAGACAGG AAGGGGACAC CTCGAAGGTC AAGGTGTGGA GAGTTGCGAA 
CAACAGAGTT GGAACCCGAA AAGGCGGGCA GCGTGGGCAG ACTCTGAAGC AGCACGAGGT 
GACGCCTGTG AGAATGTAGA CGCAACTGGA GAGAGCAAAG AAGTAGCTGC ACACAGTGAG 
GTGAACAGAG GCACGCGGCG CGGGGAGAAC GGGGACTCTG GGACGTTACT GGGCTGTGAT 
GCCGACAGGA CAGATTCCGG TGAACAATCT GCCAAGCGTC TGCGCGTCAC TCCGTGTGAT 
GCAGAGGCAT GTGGTGATGG CACCAACGGA GGCCCCCGGC CAGTTCCATC GTTCTCGTCT 
CTCTTCCTTT CACTTCCACA GCAGGAGGCT GAAGATATTC CACGCCCAGG TTCAGCTTCC 
CAATCTTCCG CAGCGTCGTC GTGCCCCCCG CTGTCCCCCG CGAACGCTTC GGGATTTTTC 
ATCGACAGTG CCTTCTCCTT CTCCCCGGTG TCGCAGGTCT CCTCATTCAG CGGGTCTCAA 
GACCCCCTCG GGCCTGACCC CTGCGGGAGC TTCCAGTCCC CATCCGCAGG GGCCTGTCCG 
GTCCCCGGTG TGCCCGCCGG TTCTCCTTGG CAGTGGCTCC ACGCTTCCAG CGCTACGGGA 
GGGGAGTCGA CAAAGGGGGC TGTAGAACGA ACCTCACTAC ACGCGGGAGG GGGCTTTGTG 
GAAGCGCCTT GGAATGCACA ATCCGCTGCT TCCACCAGAA ACCCCCCTCT GGTCGTTTAC 
CCTGAAAGAC CCACCACTGA AATGCTGCTT CCATCAGAAG TACGCATTGC AGGAGATAGG 
CCTGCTCCCT CAATAACTGG AGTGGCACCT TCCCGCTCAA ACGACACTCG ACTAGGAGGT 
CCAAGGTGGC TGGAGGATCC GACGAATGCG CTCTGTCACT TAGGCAGTCG AACGGATTCT 
GCTTCTCAAC GGAATCCATC AGAGACGCGA GAGGCACCTG TAGTCCAGTC ACCGGCCGCG 
CCTGCGCCTT CGCTTGCGCC TTCACACTCA GAGGTCTGGA GAGAAAGTGG GGTTGTTCAC 
TCTGCCACGG CACTTCCTTT GCCTCCAGTC GAACACCCCA AAGCGAGCGG ACGTGATCCT 
TTTGAGGAGC GTTTGCCTTC GCAGGATGCG CCCCTGGAGA GCTCTTACTC GGCGCCGTGG 
CGTCAGATGC CGAGCTCAGG GCACGTAGGT AGCTTCGGGG ATAAAGAGAT AAGAAGGTCG 
TGGCCGGAAT GGTCGGGGGA CAAAGTGTTT CTCGAGCTGA ACGGAGTCAA TTCAACGGAA 
GCGATCCTGA AGTCTTCGGA GCATGAGGTG CGCGAGGCGA AAGAAGGAGA AGTCGTGGAG 
CTCGCTGAAG GATGGAGAGA TCAAGACGAC GACGAGAGTG GCGGCAAGGA AGAAACGGTG 
TGTGGCGTGA GAGCTGCACA ACCGGGGAGA CTCGCGATAG AAGACATAGT TACTGTAGAA 
GCCGAGATCA AAGGAAAAGG CGCCCTAGAG GAGGAGGCAG ACTGGGCCAT CGCCGCTGTG 
CCTCACGATC GCAGTGAGGA AGAGGTACCC TCTTCAGCTG CAGAAGCGGG AGGAACAAGG 
TGCGTGCCCC TCGGGGGCGG CGTCACAGCT CTAGAGCCTT TCGAGCTGTT TGCGCCACAG 
GTCTGCAGCC GATCTCAGTC AAGTTCATCG GGGAGCAGTA GCTCAGTAGA AGGGCGCCGC 
GCGCGGAGAA GAAGAAACAG GCTACTTAGA AGCGGACAGA GCCGAAAAAC GTCTTCGCTG 
GACAGCAGCA GTTGCTCGCC GACGGTCGAC TACGACGCGA GTGCGAGAAG CAGCGACGAC 
AGCGTGTCGA GCGACCCCTG CGGGGCTCTG GACATGGAAG TCGACTCCAC GAAAAATGGG 
AAGCCAAAGG AGGGGAATGC AGAGGAATCG GATACATCTC GAGTACGCGG GGAAGAGGGC 
CAAGCCACAT TTCCTACCAA CAAGTCAGGG GAAGCAGCGA GCAAAAAGCC GTCTGCAGGA 
TTGGCGTCCG GGTTCCAGGA CCGTCAAGAA GTCTGCAGGT CACGAGCCTC TGACTTTCTA 
CACCGCAGGT GTGTTTTTTC TGGCTCCGAA GAGAAGACGG CGTGTCTCTC CACAGCCGTC 
GACATGCATA TGCCTATGTC GAATCTTTCA CTCGACGCGA AAGTAACTCT CGGCCGTGTC 
CAGACGCCAA CTGGGACGTC TACAGATGTG CGGCACGCGA CGGGGATCCA CAGCACCGGG 
AAGATGGAAG ACAACCTGCG CGAGGCGGAC CTGGTCTCGA AGCGCTTGTC GCAGGGGTCG 
GCATGCGAGG ACCATTCAGG AGATGAAGAC CGTGGAACTT CGTCATCGTA TGCGGCCACG 
GTCGTTCTCT CCAGTGGTTC GGCCGAAGGA CGAAAGGAGC GCTTTGGCGG TGAAGCAACC 
GAATCAAGAA ACAGGCAAGA GGCGCCTCCA CAGCGGGCAA AAGAAAAAGA AGAGGAGACG 
GCTTCACCAC CTACCCCTGA CCGCACACGC GAGAACACTG AAAAGCATGT GGGCATGCGT 
AGCCGCCACT CCTCTCACAC ATCGAAAGAA AAATGGGAAG GACTGGAGCA CGCTCGCTTA 
CAGAATCTGT CGGAACATGG CGACTCATCT CAGGGCCCCG TCTTGACCTT GTTATTTCCC 
CGGGGTGAGC CGAAGTCGCC TCAGGTCGTC GCCGTAAACC CCACATCCCA TGCTCTCTTG 
GATCGGCACT GTTCTGTATC CCAGCAGTCG CTGCAGGGCT GGCCAGATGA GCCCGCAGAG 
CTCCCTTTCT TTGGCGACCC TCGCGATTTG CCGGTGAGCG TCCTCCGCGC CGTTGAAGCT 
ACTCTGGGAG GGCGCCAGTT GCGTGAACAG GAGGAAACAG TTCTTGGAGC CCGTCGCGCA 
GAAAGCATCG CAGCAGCAGC AGCGGAGGAG ACTCGCCTTC TGGCCGAGCG GCGTGCCAAG 
CTAGGCAGGA AGACGAGGAG ACGCCGACGG AAGAGAAAGA ACGCTCGACA GTCGCAAGTC 
TCCTCTTCTT CGCAGCAAAC GGCTTCTCAG TCGTCGCAGT GCGCCTCTCA AGAGCCATCT 
CAGCGTGGCT CGCAGAGCTC TGGGCGCTTC GGCGTGCTTG TTGACTATCA TTGCAAACAA 
CGCCCGCATG TAGAGCGGCG TCGGAGCGGT GCTGTCTCCA CAGATGCCTT AAGCAAGCAA 
GCGCCAGGTC GAGAGGCAGG GACCACAATG GAGCCCGAAC CGGGAAGAAG CGGTTGGTTT 
GGTAGTGACG CGGGCCGCGT ACAACCGTCG CTTGTTTTGC CAGGAAAAGC TGTCCAGCCG 
GACACACTTG TTGTGGAGAT TTCGTCGTCC TCAGACAGTG GTAGCGATGG GGTCTGCAGC 
CGCTCTGCGG TTGTGCTGAC GACGTGGGAT GCGACGGGCA GAGGGCCAGG AGAGACGAGT 
TTGCCTTCCG CTCCTGTCGT GGCTATCGCC GACCGAACAA ACGGGGAGAC CTTCCCAGGG 
ACCGCGTTCC CACACGGTTC TACCAAGCCT CCAGGAAACG CTTTCTGGGG CTTCTATAAG 
CCGCCCCCCA CGACCCACGA GGCGCTCGCG AGTTGCCCCC CCAGGGTGCA GAAGAACCAT 
GAGCGGCTGT TGCGTCGGGA GGCACGCAGA GAGGCAAGGC GCCGACAGCG GCGCCAGAGC 
TGGGGGGGTG AGGCCGAGCG AGCGCGAACC GGCGACAGTT CTGCCGCGTC GCGGTGCGAA 
CGAGTGCGAG AACCTAAGGG CGACAGTAAG CGCGATTTTA AACGACAGAA CCGAAGAAGC 
AGAAGGGACT ATAGTCCGAA CAGGAAGGGA CGCGAGTGCA GAAAAGAGGT GAGGCCGGCG 
TTGAGATGGG CCACAAGGAT GGATAATGGC GGGCGTATCG TCCTGATGCT AGTGCCAGCA 
TCGCAGTCCA GCGGAGTGGA ACGAGAATCG GCACGACGAG AAAGAAGAAG TCGCACTCGA 
AGAACGATAT CGCTTTCTCG ATCCCGAGCA GAACCGACGG TGACGCGACC CTCGGAAATA 
CCGCCGGCTT CCTCATCTGC GTCTGGCTGC TGCACCTCTG AGGGCTTTAG CCATACTGGA 
GATGACGCAG CCGCCGATAG GAACTGGACC GACGAAACGA GGGCGCGTGC GGATCTTCCG 
AGTCGAGGGT TGCCGTTTGC GACTGCATGC GTGGAAGCTG AGAGCTGCCC TCAAGACGTG 
GGAAAGCCGC GAAATCCACG GAAAACAGCA GGTGTCGATG AGGTGAAGGT GGACCACGAT 
CCACCGACGT CGCCGAGCGC AAATGAACTG AAGGGGAAAC GCAGCGACTC CGATGATGAG 
GGGGAAGGTG GCGAACGCTT GGGTGCTCCT GAAAGAGCCC GACGCAACGA GGCGAACGAC 
TTGCTACCGC ATCAAGATGT TGAAGTGAAT TCTGTTCAGC CGCGCGATAG GCATATCTGT 
GACGGGACCT CTCCTGTCTG CGACAGGGAG CATGTACCTT CTTCCCAGAC ACAGCAATGT 
GGTTCGTTGT GCCTGTCAGC CGGCAGTTTG ACGACAGGCC ACTCGTTCTC AAGCGCGTGC 
TCCAGTAGCG ACGTCTCCCT GGGAGGGGTG GGAGAAGGCG CCAGGAATGT TTCGCAGCTC 
AGTGTCGCAT CAGCTCTTAA AGACGCGGCA AAACAGGCTC GGCCGTTTCA TGCCTCCGCG 
GCAAACGAGC TTGCCGACGA GGCGATTCGT GGGGAAAGGA CACTCGCCAG CTACGGCGCG 
TTGTTGGCCG TCGAAGTCCT CGCAGAGAGG ACACAATGCA GCCCTGAAGC TGTCGATGCG 
GGTGCGCCCG ATCCTCAGCA GGACGCCATC CTGGCTGTCG CTTTGGTGCT CAGAGACGAG 
CGTCTGTCTT TGGCTTGCCG TCGGGCGCAG CGGGCGAATG GGAGACACGG GGGAGACGCG 
GAAAGGTGGG ACAAGGAAGC AGAGGGGCCA GAAGACCAGG GCAAGGAGGG CTTGGGCAGA 
AGAGAGGGAA GAGAAACGGA CATGGAAATG GGAGCAAAAG GGAACAGGAA CGGAGACAGT 
CAAGAGGCGG AAAGCAGCTG CGGGGACCCA CTAGCGAACT CGGGTTCCGA GACGCAGAGA 
CGGCGGAAGA GCGGGAGCAA GCCGTTTCTG TCCACGGCCC TAGGTCGGGA GCGATGCGCA 
GAAAAGGCGG CGGCAAGTCT CGCCTATTTC GATGCTACCA TGATCATTTT TGTTGACCAG 
GCCTACAAGC CTCGCCTGCC CTTGAACTCC AGATTATGTG CTCGCTCTCC CGGATGCCGT 
TCTGCCTCTG TTCCGTCGTC ATCAACTGCT CACTCCCTTA CCAGCTCCTC GGTCGCCTCG 
CCGTTCACGC GAGCTCGCCA GTCGTTCGCG GACTTTGTCG CTCTATGGCA GTCTCGTCAT 
CCGTTCACCA AGTCTGCCCA GCAGCAACTG CCCGCCTTCC TTCCGTCTGA TACTGAAGTG 
GTTGCCGTAG AAAGCGAAAG AGCCCTCATT CATTATGTTT GTCTTATCTT TAGTGTTGCC 
GATCCTTCTA TGGTTCTGCA GTGGGACAGT GGATGCATGG GCCTCGGCTT TCTTCTCCGC 
AGGGCGCGAG TTCTCGGCTT TGGTGCAGAA CTCACAACAG CTCTCGGGAG ACGAGCAGAT 
CCTCTCCTTC TCTCACATCT CCGATCTATC TCGTATCGAC CCAGGTCGCA CGTGGCCATC 
TCTGTGTCTC CAGCGCCTGT TTCGCAGCAG TCTTTTGGCT CTGTTTCATC TTCCTCTCCA 
GCCCTGCCGC AGGCTGAATC AACCTCAGCT AGCTCGTGGT CTCGCCCGTC TATAACGGAT 
CACCGTGAGG CACGACACCC CACACCGCGA GAGTCAGGGA GGTCAAGTGG GGTCTCCGAC 
GTGCTCCTCC AAGGAGGGAT GCTGTCGGGC CGGCTTCTTC TCGAATGTTG GCGGCTGCTT 
CAGAGTGAAG TGAAACTCCA GCAGTCGTCT CTGGAAGGAG TTGCCACAGA GGTCCTTGGT 
GTGACCTTCG CCTCGGTGCC TCCGGCGATT CTTGCGAACC TCTGGAAGCA GGGACAGGGC 
GCTTTGAGGC GCGCAGAGAG GCTTGACGAA GTTGAACTCA TGACAGCGTG GCGTGAATGG 
CAGTTGCGCG TGAGGAGACA CGAAGAGGTA CAGGGTAGGC CAAGAGACAC AGCTGAGGGG 
GGTAAGGGCC ATGTTTTTCA TGGAGAGACG CAGGGTCCAC GAACAGAGGG TCCACAGACA 
GCTGATGGGG CTGGGCTACA GCACATCGAT GCAGCACAGA GAACCCTGGG CGAGCCAGAC 
AGTCTGGTCC TGGAGCAGAT CTTTGCAGAC GTTGAGCGAG CAACAGGCAA TCCGATGGGG 
CGGCTGCCGG GGCCTGAACC AAGAGAACGG GACCAAGAAG TAGTGTGGGC CGTTTCGACT 
ATGGGTGCGG TCCTCAAGTA CCTTCTGGGC AGGCCCCAGC GGCGATGTGC CCTGACGAGA 
GGGAGTCAAT TCAAGGTGGA AAGCGTGATG ATCCAAGCAA CCAAGCGCCT TGATTATCTG 
TTTGTGTCTC CTTCTCAAAC TCAGGTGACA GAGCAGCCGG CACCGCTGTG TATTCCACTC 
GTTCTCGAAC CCAAAAGTGG GTTTTACTGG AGTCCCGTAC TGGTTCTAGA CTTCCGTTCT 
CTCTACCCGT CCATCGTGAT CGCCAACAAC ATCTGCTACT CGACGGCTCT TGGCTCCGTT 
GAGATCGATC CGACAGTGCA AGCCACGAAG ACGTTGGGCG TGATGGACTT CCACTGTCCT 
CCGGCTCTGT TCCGGCAGAT TTCGGAAGCC TACTACCGAG CTGCTTCCGT GGACCTAAGG 
AAGGGAGCAC CGGCTCTTCC TGACCCTGTT GGGGGTGCTG GTGAGCGGAA GGGCGGAAGG 
GATAGTGGCG AACGACGAGG CCACAGCCAA GCTTCGAGCT TTTGTGCAAG CCAGGCGTGG 
GACGCAGACA GTGGTGGACT TCGGCTTTTG CCAAGCGGGG CCATGTTCGT GTCGCGCCGT 
GTAAGGAAGG GGATTTTCCC TCAAGTCCTC AGCGAGATCC TGCAGACCCG CATCATGGTC 
CAAAAAGCGA TCAAAAAATA CAAAGGGAAA GTGGAAGAAG GCTTGCTCCG ACTCCTAAAC 
TACAGGCAGT ATGGCCTAAA AATGATTGCT AACGTTTCCT ACGGTTATAC AGCAGCAAGT 
TTTACGGGGC ACATGCCGTG CGCCGATATT GCCGATGCAA TCGTACAGAC AGCCCGGACA 
ACGCTCATGC GCGCCATCGA ACTGGTCCAT AACACTCCAC GATGGGGCGG AAGAGTATTG 
TACGGAGACA CGGACAGCAT GTTTGTTCTG GTCGAGAACA AGTCCCTGGA AGCTGCCTTT 
GAGATTGGAC GTGAAATCGC GTACACTGTC ACTCAGCAGA ATCCGTCACC AGTTGAGCTG 
GAACTTGAAA AAGTTTTCTA CCCCTGCTGC CTCGTCTCGA AGAAGCGCTA CGTGGGGAAC 
GCGTATGCAG CGCCGAACCT GCCTCCAACC TTTGAAGCGA AGGTCAAGGC GCATCTCTAC 
CGGCAGTGGA CAAAGATTAT GTTAAACCAG GTCCCCTTGA AGGATTTCAT TTTCTACAAG 
AAATGCAGGC TCGGTTCCTA CAAAGCCGAG TTTGGCGCAT CAACTGCTGC ATCCCTACCC 
CCGCAAGCTA AAGTGGCATA TGCACGGTTG CAAGCGCTTG TTCAATCCTC TGTGTGCTTG 
CAAGATGTGC TTGGCACCGG CACGTCGGAA ACTGCTCCTG CGTTGCCGTC AGGACCATCT 
CGCGGAGAGC GAGTGGCATT TGTCTACGCG GATCTCGGCC TCGGAGGAGC AGAAGCGTTT 
TTGACAGGAA AGCAGAGCGC GTTGCGGCTG CTTGATTGTG CCGTGAGCCC TGAACAAGTC 
GAAGGGGGAG GGAGGGCGGA TAACCTTGTC AGCCTCTTCC TCTACGGTGC ACCTCAAAGG 
GTGCTGCGCC CCATGCCACT CCAGCTTCCA GACGCTTGGA CAACTGGATC GGTGACGCCA 
CCGCAAGAGG GGACCGCCAG GGACTTTTGG AGTCAGCACG CTGGCTCTTG TGTCTGGCTT 
CGATGTTGGG AGCCCATGCC ACGTTCAACA GTAGTAAAGG GTACAGACTC ATCATTGTTT 
CCTGAAGGTG AGAGAACACA AGCGGCGTCC CCAGCGTTGC AGGCGCCCGC CTCCCGCTTC 
GGCTGGCGTC GCCTTGGCAT GAGTCCAGCT GGTGCGTCCT GCTCTGGTTC GTTCTCTGTA 
TCGGTGCAGC AGGGCCAAGA GTGTAGTGGT ATTGCACCAT CTGCTGTGCA AAGCCTTGGA 
GAGGGTAATT CCGAGCAACT GCTGTTGCCA GTATATCTGA AATACTACAT CGTACGGCAA 
GTCATTCCTG CCCTCGACCG CCTCTTCGGT CTTCTTCCCG GGTCAACATC TGTAGATCTT 
CGGCAGTGGT TCGACAGAAT GCCGAAGCCT CAAACAAAGC CAGCGACGAG ATTGCGTCGT 
CAGCCACAAA CCGGCCAACA GGTAGTGAAG AAACCTAGAC AGGGCATGCT GACGTTTGGG 
ACAAGACCGC TTCCACGGAA AATAAGTCCG GAAAGCCGGT TACTACGAGC TCGACATGGG 
TCGGTGCCTC AGAGGACTCC AGTGGTAGAT CAGATGGCAG TTCCGTCGGA TGCTGGTGCG 
GTGTCGCCGG CGCCTCAGCC CCGGCACAAA TTGCTGCAGG CTTTGAAAGT AAAAAACGGG 
GCCGGTGGCG GAAGCAGAGT ACGCCTTCAC CACTTTTTTG CGGCTTCCAC CTGCCTGTTC 
TGTGGTGAGA GGTGCTCTCT GTTACCTCCT GGATCTCTCC GAGAGCTGCT GGGCTCCAGC 
GCCAGAGCAA ATGGGAGTAA AGGGAGTAGT GGAGGTCACA AAGGAGACGG GAGCACAGAC 
AAGGTGAGGC GAAGGCGCGG AGGAGGTGCA ATTCATTTTG AAGAGGACAC AGAAGCAGCT 
GTCAGAGCAC AGCTTTTCAG AAAATTTGAG TGTCGTAACC TCACCAGTGA CGAAGATGGA 
GAAGGTAACG AAAATGGGGG ACGCAATAAC GATGGAAAAT TAGTCATCGA CCTTTCGTCC 
AAGTCTGCTT TCTCTTCAAC ATCTCATGGG TCAGTCTTCT GGCCTTTGTT TCAGTCCGCG 
TCAGCCCGAG AGAAGCAACG CTACTGGCAG CCTCCTCCGG TGTGTGCAGC GTGTAGCAGG 
GATGCCGAAC GCCTTCTTGT GGTAGCATAT TCGGAGTTGC GCGAGGAAGA GAAAAGGGTA 
AAAGAGGTTG AAGACATCTG CGAGGCATGC GCAGGGAGCA GACTCTGTGC AGCGGCATGT 
TTGAACGCTT GGCACTGTGA CGTATATTTT AGAAAGCTGC GAAACGACAT GCGCCTAGCA 
GCTGTCTCCT CCAAATTACG CGCACTTCAT TTGCACCTGA AGGATAGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_264670 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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