TGME49_253930 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_253930
Entrez Gene ID	7900029
Full Name	GCC2 and GCC3 domain-containing protein
Synonyms	TGME49_053930
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018781033.1	XP_018638158.1	GCC2 and GCC3 domain-containing protein

Pathways from BioSystems
Gene Ontology

The following TGME49_253930 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_253930 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi08484	XM_018781033.1 Latest version!	Toxoplasma gondii ME49 GCC2 and GCC3 domain-containing protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_253930 ( XM_018781033.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018638158.1 Toxoplasma gondii ME49 GCC2 and GCC3 domain-containing protein partial mRNA.

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CloneID	OTi08484
Clone ID Related Accession (Same CDS sequence)	XM_018781033.1
Accession Version	XM_018781033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	GCC2 and GCC3 domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031470). COMPLETENESS: incomplete on both ends.

ATGTCTGTTC ACACACAATG CCAGACTTCA TCCTCGTTAC CTGAGTCAAG CGGTGCACGG 
CTGAAGTGTG GGGCTCTGAA TTCTGTTTTC CGTCCCTTTT TCGCCTCCAT TGCCTTGGTA 
CTGCAGTCCT CAGTGTCCAG AGATTCGATT ACCACAGCCC CGGGAGGCTT CTGGCTCGAC 
GCCAGCACCG GCTTGCTCAC TGCATGCGAA CCAGGCAGCT ACACGGTCTC CGACGGAGCC 
ACGTCGGCAG CGCACTGCGT CGCCTCTCCC GCCGGCACCT ACGTCTCTTA TTTCGGGGCT 
CCAGAAACTG AGGGCAGATG TTCGAGGGGG TACTACTGTC CTGCTGGGTC GACGTCAAGA 
GAACAGATAA GGTGTCCTTC TGGGACCTAC TCCACGTCTC CGGGAGCAGG TCGCCTGGAC 
GAGTGCGTCG CATGCCCCGC AGGCTCTCGC TGCCTCCAAA TGGCCCTCAC CGACATTCGA 
TCGGATTGTC CCCCTGGCTT CTACTGCCCT GAAGGCTCCA CGTTTCGCGG GGTCTCCTGC 
CCCGCAGGCA CGTTCAGGGC ACAGAGTCAG GGCAGAGAAC TTGCAGACTG CGTGCAGTGT 
CCTCCTGGAA TGTATTGCCC TGAACCTGGG CTCTCTGCAC CTGCCGCCCC TTGCAGCGCT 
GGATACGTCT GTCTTGGAGG TGCCAGCAGT CCTGCGCCAA TCGACAACAT AACGGGCCAA 
GTGTGCCCAG CTGGGGGGTT CTGCCCCGAG GGAGCGACCG CCAATGCGCC ATGCCCTGCC 
GGTACGTACA ATCCGCTTCC GGGGGGGAGA GACGCTTCGT CCTGTTTGCC CTGTCCTGCG 
GGTCAGTACT GTCTAGGAAC AACAAACACG ACACCCGACG GAGCCTGTGA AGACGGGCAT 
ATTTGCCCAG AAGGGTCCTC ATCTCCGACA GAGACTCCTG CGTCTCCGGG CCACTACGCC 
GTCCACCCAT TCGTCACTGA GGAAAAGTGC AGACGAGGCA GCTTCACTGA GAAATATGGC 
CAATCGGAAT GCACTCCGTG CTTGCCTGGG TCCTATTGCA ACGACGAGGG CATGTCCCGC 
GTCAAGACCT GCCCTGCAGG GACATTTTGC CCAGAAGCGT CGCTCGTCAC GCAGGAGTGT 
CCAGTCGGAA CCTACAGCCC CGTTCCCCTT CGAGGCTCCA TTGACGACTG CATTGCATGC 
CCGCCGGGCC ACTACTGCTC TAAAAGCCGC GCGACCCAAC CGAGAGGGCC TTGCGCTCCC 
GGCTTCTTCT GTCAAGGCGG GGCGACAACA GACAGGCCTG CAGAGGGTAG TGGACAAGGT 
GGGCCCTGCC CTGTTGGTCA CTACTGTCCA GCGGAAACCG AGGTGCCCAC GCCTTGCCCT 
CCCGGGACCT ACGGAGCGAT GCAGGGACAG AGAGGGCCAG GATCTTGCCT TCCTTGCCCA 
GGCGCGACGT TCTGCAGCCA AGCGGGTCTG TCGACGTTTG AGGGGCTTTG TGCAGGAGGA 
TTCACCTGCC CCTCTGGGAG CATCAAGCAA AAGCACACTC TCTGTCCTGC TGGGTCCAAA 
TGCCCGCCCG GGAGTGTCTC GCCCACGGCG TGTCCTGCAG GAACGTACCA GCCCGATGAA 
GGCCAAGAAG ACTGCTTGCC CTGCCCTCCA GGATACAAGT GCCCCTCCAG ACAGACAGCG 
TTCCTCAACC GCCCGTGTCC TGAAGGAACT GTGTGTCCTG ACAGCAGTAC CACGGAGCCG 
CAACAGTGCC CCGATGGCAC CTACAGTGTA CATACAGCGC GGATCCACAT GGAGGCATGT 
CTCCCCTGTC CGCCAGGCCT GATGTGCCAG CTGAGCAATG CCAAACCGGA CACCACCGGC 
ACCGCGGACT GTCCTGCAGG CTACGCGTGC TCTGGCGGTG TCAAAGCTAC AGGTTCCGGG 
GTAGAAGGCG AAGGAGCTGT GAGATGTGAG AAAGGCTTCT ATTGCCCTAG GGGGACTGGT 
GAACCTATAC CCTGCCCCGC TGGAAAAGTT TGTGCAGCCG CACAGCTGGG CACCGCTGAC 
GTTCCTTGCC CCGGCGGCCG CTACTGTCCG ATGCAGTCGT CGGAAGGCTT CCCTTGTCCT 
GCCGGATACT ACTGCCCTCA GGGGTCAGCA GCCCCCTTGC GATGTCCCCT GGGGACGATC 
AGCACGTACA CGGAGAACGC CGCTGAGGCA CAGTGCACAA AGTGTCCACC TGGCAAGTTC 
TGCGACGGGA CGACGAACGG GATCGAGGAC CAAATTGACA GCACCGCCTT CAGCGGAGAA 
TGCCGAAAGG GATACTTTTG CCCAGAAGGC CTGTACACCG CCATAGGCAC GCCGTGCCCC 
ATCGGCTTCC ACTGCCCCGT CGGCAGCGCC ACACCGCTCG CCTGCCCTCT GGGGTCTACC 
ACGGCTGCTG CTGGACTCTC GGAATGCACT GGCTGCGATG CAGGCTTCAT CTGCTCGGAT 
GGCAACGTGC AGCCTTGTCC TGTTGGTCAA TATTGCACTG CGGACAGTCT ACCGCAGGAC 
TGTCCCTCGG GGACCTATAA CCCCATCGCA GGAGGAATAG ACTTCGACGC TTGCCGGCCC 
TGCCCTCCTG GCAAGGCATG CAGAAGGAGC AGGACGTCAA AGCCAGAGAC ATGTCGAGCG 
GGCTACTTCT GTCTCTCGGA GGTGACGGTG CTGAACCCCA CAACTTTGTC TGAGGCGCAG 
AGCATCGCTG TTGCCGAAGG AGAAGCCGGG GCAGCTACGG AAGGCGGAGG CCGGTGCCCG 
AAGGGGTATT ACTGTCCAGC TGGGACGCGC GTGCCTCTTC CTTGCTCTGC TGGACATCAC 
TGCGGAACTC TTGGCCTGGC GGACCCGCGT GCGCCCTGCA GTGCGGGGTA CTACTGCAGA 
AGGAAAGCTA ATTCAGCGAG TCCACCTGCC CCAGCCGGTG CCACAAGCTG CCCTTCTCAA 
GACCACTATG GTGCATGCAC TCCCGGTCAT TTCTGCCCTT CTGGGTCTCG CGTCCCCGAG 
GCGTGTCCCC CCGGTACCTA TCGGTCTTTG CCCCTCGGCA AGAGTGCCGG GGATTGCGTG 
CCCTGCCCTG GGGGCCGATA TTGCTCTGGG CGAGGTCTCG TCTCTCCAAC AGGCTCTTGT 
CGACCAGGGG TATTCTGTAC GAAAGCAGCT GAGACACCGG ATGGAACCTT ATGTCCCGAG 
GGACACTATT GCCTGGAGGG AACAGTGTCG CCAAAGCCTT GTGCAGCAGG AACATACCAG 
GCGACGGTGG GACACACGAG CTGTAGGACC TGCCCCAAGG GGTTCCTCTG CGGCCCTGGA 
ACGGGCACGG TGACTCCAGT GGCCTGTCCC CCGGGTCACT TTTGCCCAGC GTCCACTTCA 
AAGCCGTCCA AGTGCCCTGC TGGAAAGTAT TCCCTCGTCG ATGGCCTAGC GGAAGAAGCG 
AATTGCTCGT GGTGCCCTCC TGGAAATGTC TGCACGGCAG AAGGCGTTGC CACAGAACTC 
AAACGGTGCC CGGCCGGGAG CTACTGCCCA GCTCCGGACT ACGTCCTTTC ACCTGCCACT 
GGAGGCGACG GCAGCAGTGG GGCTTTGAAG GTCACCTGCG CCTCCTCAAC TGGCGGCGTT 
CCCACCTGCG CCATTGACGC ACTTGAGTGT CCTCTTGGTT ACGTTTGCCC AGAGGGCAGC 
GCAACTGCCA AAAGATGCCC CCTAGGCCAG ACCGTCGCCG TGAGCGGCGC AGCGACCAAC 
TCCGGATGCA AGCCGTGTCC AGCGGGACAG TTCTGTTCTC CCTCAGGAGC CGTCGAGCCG 
TGTGCCGATG GCTTTCTCTG CATTTCTGGG GCGTATCGGC CGAAGCCGTT GACCGCGAAA 
GTCGGACGTC TGTGCCGGGC TGGACATTTC TGTCCGTCGG GCACCACCGT CGAAATCGCG 
TGTCTCCGGG GCACTCTCAG TGTCGGAGAA GGAAGTGGAA ACGCCTGCGA CGAGTGTCCG 
CCTGGGAATG TCTGCGATGC AGAAGGCATC AAGGCTCTAG ACGACCATCG GGCCTGTCCA 
GCGGGCTCTT ACTGCCCACC TGGTAGTGTC ACAGCGATTC CTTGCCCTGC TGGGACGTAC 
AGCGCCTTCA CATATCTCGC TTCCTCTCAG GAATGCCTAG CCTGTCCTGC AGGAAAGTAC 
TGTGAGAGTG CGGGAGCCCA CGCCGTGACT GGCCCCTGCG ACGCAGGCAC ATGGTGCAAG 
CGAAAATCGA CGAAGCAAAA TCACTCAGGT GGATACGCGC CATCGAGCCT CGTTGCCTGC 
CCTCAAGGTG CCTATTGCTT ACAGGCCGCA CCCATGCCTA TCCTGTGCCA ACCGGGGACC 
TACCAGGACG AGGAGGCGAC CGCCGACGCG TGCAAGCCGT GCGCTGCAGG CACCTTTTGT 
GGGTCTGCTG GCCTCACGAC GGAAGCAGGA AGCGGCCCTT GCCAAGCAGG CTTCTTCTGC 
AAGGAAGGCG CCAAGGTCGC CGCACCGAGA GACAAATCAA CTGGGGATAT CTGCAAAAAG 
GGCCATCATT GCCCGTCAGG ATCCAAGGCA CCTCAAGCGT GCCCCCCCGG GTACTACAGT 
GACGAGGAAG GTCTGGCCAC ATGCAAGCCA TGCCCTGCAG GCTCTCTCTG CCCTGGAGGA 
ACCAACGGGG AAATCGGGGG CTGCCCTGCT GGATCCTTCT GTAAGGCGGC TGTCTCGGCT 
GCTGCGGCAT GCCCCGATGG AACGACAAGC GAAGTGGAGA GTCTTGCGGA AGAAGGCCAG 
TGTGCCGACT GTCCAGCTGG AAAAGTGTGT GAGGCTGGCA AGCCGCCAGC CTCGTGTCCC 
GAGGGCTTCC TTTGTCTTGC GGGAGTCGGA CCCAAACACA TCGGAGCTTC GAACGCCGAT 
ATCGATGAAC TGTGGCTGGA CTCGACCTTC TGGGGTGGAC ATGCGATTCA CTACGGCGGG 
GCTTGCCCGC CTGGGCATTT TTGCCCCCGT GGGGCAACAG CCCCTGAGCC GTGTGCCCAA 
GGAACTGTTC GCGACCAAAC CGGAGGCAAA AGCCAGGCCG ACTGCACCAA GTGCCCCATC 
GGCAGTTTCT GCGATCCACG GATGCCGGTC CCTGTTTCCT GCCCCCCTGG ATACTACTGC 
CCCGAGGATA CCCAGGAGCC GTCTCCCTGT GAGGCTGGAT CTTATCTTAC CGCCAGCGGC 
CACTCCGAGA AGTCCGCCTG CACCATTTGC CCGGAGGGTT ATGCATGCAA TCGGGACGGC 
GTCGTAGACT ACAAGCTCTT CCCTTGCCCC ACAGGCTACT TTTGTGGCGC TGGAGTCCTG 
GAGCCTGAGG CGTGCCCCGC CGGAACTTAC GGGGCAGCTC CGAAGCTCAC CCGGGCAGAC 
CTGTGTGAAG CCTGTCCGCC TGGAATCGTT TGTACAACGC GCGATGAGGA GGACGGTTCA 
CGGCGACCAC AGGCGCTCGG CTGTCCAGCT GGTTATGTTT GCCCGAAAAG CACAAGTACG 
CCAACACTCT GTCCTGCCGG CACCTTCTGC CCCCGAGGCA CATCGACCCC CCGCACATGC 
CCGGCTGGCT ATTTTTGCCC GGAAGGAGCA GAAACTCCTG TCCTCTGTTC CAAAGGAACG 
TTCTGCTTAG AAGGCGCAAC TGAGCCCAGT GTGTGCCCTG CAGGCTATAA GGCAAAGGAC 
TCCTGTGTAG GCGCGTACTC CGTCGAGGCC TGTTGTGAGC CGTGCCCAGT GGCCACCTAC 
TCCGACAGGC CAGGAGCTAC ACACTGCAGC CCGTGTGCAG CAGGCTACAT ATGCAAGGGC 
GGTGCTACCA CGGCGAAACC CAGCGCAGAA GAGACGAGCT CTGAGGGATA CGTGTGTCCA 
CCGGGCTACT ACTGTCTGGA GGGAGCTTCG GAGCCTACCC CGTGCCCCGC CGGCACCTTC 
AGAGACCAGC CAGGAGGCGA GGCTCTCGAC GGCTGCTCTC CGTGTCCCCA CGGAACCTTC 
CAGAGGTTTC CTGGAATGGC GTCGTGCACC AAGTGCGGGG AGCATGCAGT CTCTGAGAAA 
TCCGTGGGTT CAACAACATG CACGTGCCTC GGCATCTCTC GGTTTTACCA AATCGAAGAC 
GGCACCTGTC CCTGCTACGC TCGCTCCTCC CCTCAAGGCG GAGAGCCTCA AGAGTCACAG 
GATGGCCGCA GTCCTTGTGA AGCGAAGCTA TACCCACGTT GTGCGTCGGG CAGCCACAGG 
GACGACTTTG GCGCCTGCGT GTTCGAGGCC GAAGTCGAGT GTGGCGAGTC ATGCCGTGGA 
GGCTCTGGAA GCTTTCTCGC TGCATTTGGT GTTTGCCAGT GCGCTGATGG ACCTCGCATT 
GCAGCCCAGT GTACATCCAC CTGCAAAGAG CAGCTGCCAC AGGTGTTCGT CTCAGATGCA 
GGCTTCGAAG AAATCGACTC TGTTCAGGGA TCCAAAACGG TCTACAGCGA GGGCCTTCCT 
CCCGACATCG ACCTCGCCCA TCTGCAGTTC TTGTGCAACA AACTCAAGGA GGGTCTGCGG 
GGCTTGCCGT TAACGTCGCT CTGCGGCGTC AGCTTTCATC AGGCTTCGTC TGCCGGTCTT 
GACGGGCTTC TGCGGATGCC TGAAGAATTT CGACCGGCGC ACGGTTCTCG AAATTATGAA 
GACTCTCAAG ACACTTTCGC TGGAACGCAA GTTCAGCTCC GGCGCTCCAT TTCTTCGGCT 
TCCTTCAAGA GGCCAGGGAG TCGCCAGCGA GCTACGGACC CGCGGCACGT TGTCCCCAGG 
CCAGTTCAGT GTCTCCAACG GGGGTCAACG ATCATCTGGG AACTCAAAGA TGGCGTCTAT 
CCGGTTTACC ACAAGGAGAG TCTGATGAAT ACGCATCCGG CAATGGATGA CGGCCCGTTT 
CGAGAACTTG CGCAAACTGC TCCGATCCTC CCGCAGTTCT TCGCCTACAC ATTTCTCGTC 
GCGGGCTCCT TCGTTTTTGC AGCCTCCGAC AATCCTCTGG AGATTGCCGT CGTTGTCGTT 
GTCCCCGACG GCGTCAGATG CCCAGCAGCA AGCACGTTCG CCGCTCCGCT GTCAACGCAA 
AGCCTGGGGA TTCTCGGCGT AACGACGGCA TCGCAGCGAC TCATGACAGA GCCCAACTGG 
GGTGTGGTGT TTCTGGGAGT TTTTGCTGTC GCTGCATGCT GTCTCCTCGC CTTCTACGCA 
GTCTGGAGGT GCAGACACAG CGCCTGGGAA GTGCGTGTCT GGACTGCGCC CTTGGGATCC 
GCGAACCGAA TCCCTAACGA CGACTTTAAG TCGGTTCTCG CCGCTTTGGA CGGTCTCCCC 
GGCGCAACGG GCAACCTGCG GTGCACAGAC ACTCGGCTAG TACACCACGT CGTCGTGAAA 
CCTGCTGAGC CACTGCTGGA ACCTACGGGA AAAGCAACAA GTCCATCCGA TGACGCGCCG 
ATCATTCCTC TCAAGGATGC GGCAGATGCG CTGGTTGTCG ACATCGAGTC TACCGACACA 
CGGCTTCTGC AGGTCATGTT CGAGAAGCTT CTTGATTTTG GCAATATCCT GAAGCGTCTC 
GTCGAGGATG AAGTGAAGGC AGTTAAAGCC GCCTGCGACG TCATGCCTCA AACGCTTGAA 
AGAATTCTAA GGCGGACTCG ACAAACGCTT CTGCTGCTGC CAAACGAGCA CATGTGGGCC 
ATCTCTCCGA GTCAGGCAAA ACTGCTGCAG GCCCTTGCAT GCGTCTTCAC CGAGGGCGAC 
AACTCTCCCG CCGTCGGAGA GGCGCTGGCC AACGCGCTGA CTCCTCATAC TTTGGAGAGT 
TCTCAGGTCT CAAAGGGAGG AGACCTTCCC GGTTCGCTTC TCGCCTGCGT CGACCTCGCT 
GGCGTCGCTG TCTTCGATGC CTTCGATTGC TTGTGGCTTC TCGAGCCTCA GCACGGCGAG 
GACTCGGTGG CCGACCGTGT GCTTGGAAAG CTCGAGAAGT TGTTGGAAGG CAACGACCCC 
CTGGAGGAGA ATTCCGACTC ACTCTTCGTT ATTTACGACA CCTTGTTTGC GGAGCGGGCT 
TATCAGGAAG AGCGCTTGCG GCGGGAGTGC ACGCGCGTCC AGTCGCGTTT TGCAGGGGTG 
ATGCAGAAGT TCAAAGACAC CTGCCAGACT CTTCACCTTC TGCCGGCGAA ACAGGCCCAG 
GCGTGGTTCG AGCTGAAAGA TTTCCAGCTG CGAGAGCTGC TGGACTTCGA AGCCAAAGCC 
TCCAGGAGCA TCGTCGAGGC CGCCGTGGGG GGAGTGCGGA GCGGAAAGGC GATGCTGAAT 
GACAACCTCT TGACGACGAC TCTGGAGATG GACTCCTCTA ACAGTCGCGC CTTTCTCTCG 
GGGATTCTGG CCTCTTCCCG GAAAACGCTT CTTGACCAGA TTCGCCAGCA ACTGGCAGCG 
GCCCTGAAGG AGATGAAGCA GCTGCTGAAG AGACAAGACG CCGCGTTGAT GGAGCTCCTC 
AGTGAGCACG CTCGAAGTTA CCGTACTGCA GCTGAGGCGC GACGCCACGC ATGCAGAGCG 
CTGCTCCAGC AGAAGCACAC AGAAGAAAAA CAGAGGCTGA AGCGGTGGAG TGCCACGATG 
GTCAAGGCGG TTAAGGAGAC CCGCAATGTG CCAGCAGAGA TGCAAGGGAA CGCGCGAGAG 
GAACAGGAAC GTCTGGATAT GGAGACAGCT TTGGAGCAAG TCCATCGTGC CACTGCTCAC 
AGACGACAGC TCGGCGAGGA TCGCCAACGC CTAGAAAATC AGATGGCTGA GGCCGCTTTC 
AGCACCGCGG TGGACCTACA CGAGGCGCAC CTCGACTTTC TTCGCGACCA GACCCGAATG 
GAGTACCAGC TTCTCGAGGC GTCCTCTAGG GAAGACCACG AGATCCACAG CGACTTCGCT 
GTCGAGTTGG GTACGCTGCA GCTGGAGGCG CTCGTGATGA ACGTGATGAA GAGAGCAGCA 
AGGCAAGAGG CAAACGACCA ATGCGAGACA ACTCTCGACG AAGTCCTGGA GACTGAGATG 
GAAGGCTGGA ACGGACAGAC AGTGGTGACA GCGCTCCAGG CTGTCCAGAG CAAAGTCACG 
AAGCAGCAGT CGGCTAGCCG CGAGATCAGC GCGAGTCTCG TTGCCAAGGG TAAACAAGCT 
TCTGAGGCTC TCACGCGCTG TGTGGCAAAC CGATACGATC TTATGACACA AGCAATCGAC 
CAAATTCAAG GAAAGTCATT CCAACTTGCT CTCAGCGCTT TGGAGGCGCG GGAAGACACG 
TGGAAAGCAG CTCTTTCCGC GGCTGGCAGA CTAGCACTGA ATGACGCGGA GCGACAGACG 
TCGGAGAAAG AAGTCCTGCG GCAACTCGAG GTTCAGAAAA CAGTCGAGGC AGACGACGAG 
TTTGAAAAAA CTCAACTTCT GCAGGAGTTA CTTGAGAAGA ACTGCCACAC TCGTCTACGG 
ACTGTCACGC AGCTACGCCG TGCCATGCTT CAGACGAGGT TGAAAGCGTG GGCGGCTGTG 
GCTCTTCATG GCGGATTCTT TGACAAAGTG AAGGCGCTCC AGAGCTGGAA AGAAGTGGCA 
GACCACCGGA GTCAAGAGGA CGACGCTCTT GCAGCAATTC GTGACCAGCG CCGACAGGCA 
ATGGGGAAGT ACGTGATGCG AGTTCGGAAA CTGGTGATTC GTGAGGAGAA GCGCAGAGTC 
CACCAGAAAG AATCCCTCGC TCACGCTGCC TTCGTAGTGA CCCAGCCTCG AACAGTGACA 
GGGAAGGCGC TGGGCACTGC TGGGGGGGTG CTGCAGGGTG CGGTGGAGAT GCTGGCGCAC 
AAGAAGCTTG ACGATGAATT TACGCGGAGG CGGAGTGCCC TGAAGGCAGA GCTCAGGCGA 
GAGTTGGAGG CCATTGACGA GGAGATAAAG CGACATCAGG AGATAATCGA AGCAGAGGCG 
GAAGACCTGT CAGGAGCCGA CGATGAGGTG CCTCAAGAAA CTGTGTCGAT GGGGGGATCC 
GTGTCGGAGC TCGGAAACGC TGAAAGAGGT CAATCCGTCC GCAAAAAGCG CAACAGCGAA 
ATGGAAAACC GTCTTAGTCT CCTGGATCGT CAACGCGCAG AGATTGTTAA CCTTTTCAAC 
GAAAACGAAC GCCAGCTCGA ATCCGAGTGG CGTACGCGGG CCGTCCATGT GCGGCATGAG 
ATCCTGCAGA AAATCACTGA CTTTGCTGTT GAGTTGGAGT CTAGTGGCCA GCAGCTGGAC 
AGCATAGCCG CTGAGGTTGC CAGAGATGTG ACCGTCCAGT ACCAAGAAGA ACTGAAAGAA 
CTGGAAGGAA CGCGGGATGC ACCTGCTGCC GAACAGGACA ACACCGATGG TTTCGAGGAA 
GACAACAAGG ATGCGGACGC TGCCCCTCCT GAAGGTGACC TAGACCAGAA ACTTCGGCAT 
CTGGCGATGG TGCGGTGCCA GAGGGTAATT TTGCGCCTAA AGCGGCTAGT GGAGAAAGGC 
AGCAAGATGG CTCGAAGCCT GTCTGAGGAG GCTTCTGCCG AGCTGAGACG TCTGCAGAAG 
GAGGAGAGGG AAAAGGAAGC CGAAAGGCGC GTCGAGCTTG AGCGGGAGCT GCAACAGCAC 
GAACGCGAGA AGGTCGAAGC AGAAAAACGC CTCGCGGAGT CCCTGGAGTC TCTGCAGAGA 
GAGTGGGAAG AGAGACTGGA GAGGACCCAA GAAGAGGGGC GGGCTCGGCT ACGGGAGCTG 
CAGGAGGCGG CGCGACGCGA AGCTGGACGG AAAGTAGGCG ACCAGAATCT CGGAGCGATA 
AAGCAGGAAA TCTTCGCGAG ACACCAGGAG GACGCCACGC GCTTTGATGA GGCCCTCGGT 
GCTGAGTATT GGAAGCAAAT GAATCGGCTG AGGGAGAGAC TGCAGGTGAA GATGATGGCG 
CGTAAGAGAC GCCTGGCGGC CGCAGCGCAG GAACAACTGA GTATAGAAGG GGACCGTTTG 
AAGGAAGCGA GGAACGCCTT CGTTGCACAG CAAGAATCCA TCAGTTTTTA CGATCCTGAC 
AAACAACGAG AGGTCGTCTT GCGAATCGGG CGGGCCTGGC GAGATGAAGC TCGCCGACGG 
ACAGCACAGA AGAAAGCAAA TGCAGAAGCA GATCAAATTG CCACAGAGGA CGCCGAACCC 
CAGGCAGACT TGCCGCCTCT TCAGAAACTA TCAACGTCGG TGCTCAACAA ACGCCTTGCA 
GGAATGCGTC GTGCGATTGA GGACACCATC GCTGAGATGC ACGGTACCAT GCAGTCGATC 
TCAACGACAG ATGGTCGGCG AAGCACATCG AGCCGGCCCA CTATTCACCA ATTTGAGAGT 
CTTGTTGATG CAACGGGTCA AATAAGTCAG CTACTTCAAA ACCTGGAAAA ACTGAGCGGC 
GTGTACTCTT CTATTATGCA CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018638158.1
CDS	1..11307
Translation	MSVHTQCQTSSSLPESSGARLKCGALNSVFRPFFASIALVLQSSVSRDSITTAPGGFWLDASTGLLTACEPGSYTVSDGATSAAHCVASPAGTYVSYFGAPETEGRCSRGYYCPAGSTSREQIRCPSGTYSTSPGAGRLDECVACPAGSRCLQMALTDIRSDCPPGFYCPEGSTFRGVSCPAGTFRAQSQGRELADCVQCPPGMYCPEPGLSAPAAPCSAGYVCLGGASSPAPIDNITGQVCPAGGFCPEGATANAPCPAGTYNPLPGGRDASSCLPCPAGQYCLGTTNTTPDGACEDGHICPEGSSSPTETPASPGHYAVHPFVTEEKCRRGSFTEKYGQSECTPCLPGSYCNDEGMSRVKTCPAGTFCPEASLVTQECPVGTYSPVPLRGSIDDCIACPPGHYCSKSRATQPRGPCAPGFFCQGGATTDRPAEGSGQGGPCPVGHYCPAETEVPTPCPPGTYGAMQGQRGPGSCLPCPGATFCSQAGLSTFEGLCAGGFTCPSGSIKQKHTLCPAGSKCPPGSVSPTACPAGTYQPDEGQEDCLPCPPGYKCPSRQTAFLNRPCPEGTVCPDSSTTEPQQCPDGTYSVHTARIHMEACLPCPPGLMCQLSNAKPDTTGTADCPAGYACSGGVKATGSGVEGEGAVRCEKGFYCPRGTGEPIPCPAGKVCAAAQLGTADVPCPGGRYCPMQSSEGFPCPAGYYCPQGSAAPLRCPLGTISTYTENAAEAQCTKCPPGKFCDGTTNGIEDQIDSTAFSGECRKGYFCPEGLYTAIGTPCPIGFHCPVGSATPLACPLGSTTAAAGLSECTGCDAGFICSDGNVQPCPVGQYCTADSLPQDCPSGTYNPIAGGIDFDACRPCPPGKACRRSRTSKPETCRAGYFCLSEVTVLNPTTLSEAQSIAVAEGEAGAATEGGGRCPKGYYCPAGTRVPLPCSAGHHCGTLGLADPRAPCSAGYYCRRKANSASPPAPAGATSCPSQDHYGACTPGHFCPSGSRVPEACPPGTYRSLPLGKSAGDCVPCPGGRYCSGRGLVSPTGSCRPGVFCTKAAETPDGTLCPEGHYCLEGTVSPKPCAAGTYQATVGHTSCRTCPKGFLCGPGTGTVTPVACPPGHFCPASTSKPSKCPAGKYSLVDGLAEEANCSWCPPGNVCTAEGVATELKRCPAGSYCPAPDYVLSPATGGDGSSGALKVTCASSTGGVPTCAIDALECPLGYVCPEGSATAKRCPLGQTVAVSGAATNSGCKPCPAGQFCSPSGAVEPCADGFLCISGAYRPKPLTAKVGRLCRAGHFCPSGTTVEIACLRGTLSVGEGSGNACDECPPGNVCDAEGIKALDDHRACPAGSYCPPGSVTAIPCPAGTYSAFTYLASSQECLACPAGKYCESAGAHAVTGPCDAGTWCKRKSTKQNHSGGYAPSSLVACPQGAYCLQAAPMPILCQPGTYQDEEATADACKPCAAGTFCGSAGLTTEAGSGPCQAGFFCKEGAKVAAPRDKSTGDICKKGHHCPSGSKAPQACPPGYYSDEEGLATCKPCPAGSLCPGGTNGEIGGCPAGSFCKAAVSAAAACPDGTTSEVESLAEEGQCADCPAGKVCEAGKPPASCPEGFLCLAGVGPKHIGASNADIDELWLDSTFWGGHAIHYGGACPPGHFCPRGATAPEPCAQGTVRDQTGGKSQADCTKCPIGSFCDPRMPVPVSCPPGYYCPEDTQEPSPCEAGSYLTASGHSEKSACTICPEGYACNRDGVVDYKLFPCPTGYFCGAGVLEPEACPAGTYGAAPKLTRADLCEACPPGIVCTTRDEEDGSRRPQALGCPAGYVCPKSTSTPTLCPAGTFCPRGTSTPRTCPAGYFCPEGAETPVLCSKGTFCLEGATEPSVCPAGYKAKDSCVGAYSVEACCEPCPVATYSDRPGATHCSPCAAGYICKGGATTAKPSAEETSSEGYVCPPGYYCLEGASEPTPCPAGTFRDQPGGEALDGCSPCPHGTFQRFPGMASCTKCGEHAVSEKSVGSTTCTCLGISRFYQIEDGTCPCYARSSPQGGEPQESQDGRSPCEAKLYPRCASGSHRDDFGACVFEAEVECGESCRGGSGSFLAAFGVCQCADGPRIAAQCTSTCKEQLPQVFVSDAGFEEIDSVQGSKTVYSEGLPPDIDLAHLQFLCNKLKEGLRGLPLTSLCGVSFHQASSAGLDGLLRMPEEFRPAHGSRNYEDSQDTFAGTQVQLRRSISSASFKRPGSRQRATDPRHVVPRPVQCLQRGSTIIWELKDGVYPVYHKESLMNTHPAMDDGPFRELAQTAPILPQFFAYTFLVAGSFVFAASDNPLEIAVVVVVPDGVRCPAASTFAAPLSTQSLGILGVTTASQRLMTEPNWGVVFLGVFAVAACCLLAFYAVWRCRHSAWEVRVWTAPLGSANRIPNDDFKSVLAALDGLPGATGNLRCTDTRLVHHVVVKPAEPLLEPTGKATSPSDDAPIIPLKDAADALVVDIESTDTRLLQVMFEKLLDFGNILKRLVEDEVKAVKAACDVMPQTLERILRRTRQTLLLLPNEHMWAISPSQAKLLQALACVFTEGDNSPAVGEALANALTPHTLESSQVSKGGDLPGSLLACVDLAGVAVFDAFDCLWLLEPQHGEDSVADRVLGKLEKLLEGNDPLEENSDSLFVIYDTLFAERAYQEERLRRECTRVQSRFAGVMQKFKDTCQTLHLLPAKQAQAWFELKDFQLRELLDFEAKASRSIVEAAVGGVRSGKAMLNDNLLTTTLEMDSSNSRAFLSGILASSRKTLLDQIRQQLAAALKEMKQLLKRQDAALMELLSEHARSYRTAAEARRHACRALLQQKHTEEKQRLKRWSATMVKAVKETRNVPAEMQGNAREEQERLDMETALEQVHRATAHRRQLGEDRQRLENQMAEAAFSTAVDLHEAHLDFLRDQTRMEYQLLEASSREDHEIHSDFAVELGTLQLEALVMNVMKRAARQEANDQCETTLDEVLETEMEGWNGQTVVTALQAVQSKVTKQQSASREISASLVAKGKQASEALTRCVANRYDLMTQAIDQIQGKSFQLALSALEAREDTWKAALSAAGRLALNDAERQTSEKEVLRQLEVQKTVEADDEFEKTQLLQELLEKNCHTRLRTVTQLRRAMLQTRLKAWAAVALHGGFFDKVKALQSWKEVADHRSQEDDALAAIRDQRRQAMGKYVMRVRKLVIREEKRRVHQKESLAHAAFVVTQPRTVTGKALGTAGGVLQGAVEMLAHKKLDDEFTRRRSALKAELRRELEAIDEEIKRHQEIIEAEAEDLSGADDEVPQETVSMGGSVSELGNAERGQSVRKKRNSEMENRLSLLDRQRAEIVNLFNENERQLESEWRTRAVHVRHEILQKITDFAVELESSGQQLDSIAAEVARDVTVQYQEELKELEGTRDAPAAEQDNTDGFEEDNKDADAAPPEGDLDQKLRHLAMVRCQRVILRLKRLVEKGSKMARSLSEEASAELRRLQKEEREKEAERRVELERELQQHEREKVEAEKRLAESLESLQREWEERLERTQEEGRARLRELQEAARREAGRKVGDQNLGAIKQEIFARHQEDATRFDEALGAEYWKQMNRLRERLQVKMMARKRRLAAAAQEQLSIEGDRLKEARNAFVAQQESISFYDPDKQREVVLRIGRAWRDEARRRTAQKKANAEADQIATEDAEPQADLPPLQKLSTSVLNKRLAGMRRAIEDTIAEMHGTMQSISTTDGRRSTSSRPTIHQFESLVDATGQISQLLQNLEKLSGVYSSIMHN

Target ORF information:

RefSeq Version	XM_018781033.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 GCC2 and GCC3 domain-containing protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018781033.1

ORF Insert Sequence:

ATGTCTGTTC ACACACAATG CCAGACTTCA TCCTCGTTAC CTGAGTCAAG CGGTGCACGG 
CTGAAGTGTG GGGCTCTGAA TTCTGTTTTC CGTCCCTTTT TCGCCTCCAT TGCCTTGGTA 
CTGCAGTCCT CAGTGTCCAG AGATTCGATT ACCACAGCCC CGGGAGGCTT CTGGCTCGAC 
GCCAGCACCG GCTTGCTCAC TGCATGCGAA CCAGGCAGCT ACACGGTCTC CGACGGAGCC 
ACGTCGGCAG CGCACTGCGT CGCCTCTCCC GCCGGCACCT ACGTCTCTTA TTTCGGGGCT 
CCAGAAACTG AGGGCAGATG TTCGAGGGGG TACTACTGTC CTGCTGGGTC GACGTCAAGA 
GAACAGATAA GGTGTCCTTC TGGGACCTAC TCCACGTCTC CGGGAGCAGG TCGCCTGGAC 
GAGTGCGTCG CATGCCCCGC AGGCTCTCGC TGCCTCCAAA TGGCCCTCAC CGACATTCGA 
TCGGATTGTC CCCCTGGCTT CTACTGCCCT GAAGGCTCCA CGTTTCGCGG GGTCTCCTGC 
CCCGCAGGCA CGTTCAGGGC ACAGAGTCAG GGCAGAGAAC TTGCAGACTG CGTGCAGTGT 
CCTCCTGGAA TGTATTGCCC TGAACCTGGG CTCTCTGCAC CTGCCGCCCC TTGCAGCGCT 
GGATACGTCT GTCTTGGAGG TGCCAGCAGT CCTGCGCCAA TCGACAACAT AACGGGCCAA 
GTGTGCCCAG CTGGGGGGTT CTGCCCCGAG GGAGCGACCG CCAATGCGCC ATGCCCTGCC 
GGTACGTACA ATCCGCTTCC GGGGGGGAGA GACGCTTCGT CCTGTTTGCC CTGTCCTGCG 
GGTCAGTACT GTCTAGGAAC AACAAACACG ACACCCGACG GAGCCTGTGA AGACGGGCAT 
ATTTGCCCAG AAGGGTCCTC ATCTCCGACA GAGACTCCTG CGTCTCCGGG CCACTACGCC 
GTCCACCCAT TCGTCACTGA GGAAAAGTGC AGACGAGGCA GCTTCACTGA GAAATATGGC 
CAATCGGAAT GCACTCCGTG CTTGCCTGGG TCCTATTGCA ACGACGAGGG CATGTCCCGC 
GTCAAGACCT GCCCTGCAGG GACATTTTGC CCAGAAGCGT CGCTCGTCAC GCAGGAGTGT 
CCAGTCGGAA CCTACAGCCC CGTTCCCCTT CGAGGCTCCA TTGACGACTG CATTGCATGC 
CCGCCGGGCC ACTACTGCTC TAAAAGCCGC GCGACCCAAC CGAGAGGGCC TTGCGCTCCC 
GGCTTCTTCT GTCAAGGCGG GGCGACAACA GACAGGCCTG CAGAGGGTAG TGGACAAGGT 
GGGCCCTGCC CTGTTGGTCA CTACTGTCCA GCGGAAACCG AGGTGCCCAC GCCTTGCCCT 
CCCGGGACCT ACGGAGCGAT GCAGGGACAG AGAGGGCCAG GATCTTGCCT TCCTTGCCCA 
GGCGCGACGT TCTGCAGCCA AGCGGGTCTG TCGACGTTTG AGGGGCTTTG TGCAGGAGGA 
TTCACCTGCC CCTCTGGGAG CATCAAGCAA AAGCACACTC TCTGTCCTGC TGGGTCCAAA 
TGCCCGCCCG GGAGTGTCTC GCCCACGGCG TGTCCTGCAG GAACGTACCA GCCCGATGAA 
GGCCAAGAAG ACTGCTTGCC CTGCCCTCCA GGATACAAGT GCCCCTCCAG ACAGACAGCG 
TTCCTCAACC GCCCGTGTCC TGAAGGAACT GTGTGTCCTG ACAGCAGTAC CACGGAGCCG 
CAACAGTGCC CCGATGGCAC CTACAGTGTA CATACAGCGC GGATCCACAT GGAGGCATGT 
CTCCCCTGTC CGCCAGGCCT GATGTGCCAG CTGAGCAATG CCAAACCGGA CACCACCGGC 
ACCGCGGACT GTCCTGCAGG CTACGCGTGC TCTGGCGGTG TCAAAGCTAC AGGTTCCGGG 
GTAGAAGGCG AAGGAGCTGT GAGATGTGAG AAAGGCTTCT ATTGCCCTAG GGGGACTGGT 
GAACCTATAC CCTGCCCCGC TGGAAAAGTT TGTGCAGCCG CACAGCTGGG CACCGCTGAC 
GTTCCTTGCC CCGGCGGCCG CTACTGTCCG ATGCAGTCGT CGGAAGGCTT CCCTTGTCCT 
GCCGGATACT ACTGCCCTCA GGGGTCAGCA GCCCCCTTGC GATGTCCCCT GGGGACGATC 
AGCACGTACA CGGAGAACGC CGCTGAGGCA CAGTGCACAA AGTGTCCACC TGGCAAGTTC 
TGCGACGGGA CGACGAACGG GATCGAGGAC CAAATTGACA GCACCGCCTT CAGCGGAGAA 
TGCCGAAAGG GATACTTTTG CCCAGAAGGC CTGTACACCG CCATAGGCAC GCCGTGCCCC 
ATCGGCTTCC ACTGCCCCGT CGGCAGCGCC ACACCGCTCG CCTGCCCTCT GGGGTCTACC 
ACGGCTGCTG CTGGACTCTC GGAATGCACT GGCTGCGATG CAGGCTTCAT CTGCTCGGAT 
GGCAACGTGC AGCCTTGTCC TGTTGGTCAA TATTGCACTG CGGACAGTCT ACCGCAGGAC 
TGTCCCTCGG GGACCTATAA CCCCATCGCA GGAGGAATAG ACTTCGACGC TTGCCGGCCC 
TGCCCTCCTG GCAAGGCATG CAGAAGGAGC AGGACGTCAA AGCCAGAGAC ATGTCGAGCG 
GGCTACTTCT GTCTCTCGGA GGTGACGGTG CTGAACCCCA CAACTTTGTC TGAGGCGCAG 
AGCATCGCTG TTGCCGAAGG AGAAGCCGGG GCAGCTACGG AAGGCGGAGG CCGGTGCCCG 
AAGGGGTATT ACTGTCCAGC TGGGACGCGC GTGCCTCTTC CTTGCTCTGC TGGACATCAC 
TGCGGAACTC TTGGCCTGGC GGACCCGCGT GCGCCCTGCA GTGCGGGGTA CTACTGCAGA 
AGGAAAGCTA ATTCAGCGAG TCCACCTGCC CCAGCCGGTG CCACAAGCTG CCCTTCTCAA 
GACCACTATG GTGCATGCAC TCCCGGTCAT TTCTGCCCTT CTGGGTCTCG CGTCCCCGAG 
GCGTGTCCCC CCGGTACCTA TCGGTCTTTG CCCCTCGGCA AGAGTGCCGG GGATTGCGTG 
CCCTGCCCTG GGGGCCGATA TTGCTCTGGG CGAGGTCTCG TCTCTCCAAC AGGCTCTTGT 
CGACCAGGGG TATTCTGTAC GAAAGCAGCT GAGACACCGG ATGGAACCTT ATGTCCCGAG 
GGACACTATT GCCTGGAGGG AACAGTGTCG CCAAAGCCTT GTGCAGCAGG AACATACCAG 
GCGACGGTGG GACACACGAG CTGTAGGACC TGCCCCAAGG GGTTCCTCTG CGGCCCTGGA 
ACGGGCACGG TGACTCCAGT GGCCTGTCCC CCGGGTCACT TTTGCCCAGC GTCCACTTCA 
AAGCCGTCCA AGTGCCCTGC TGGAAAGTAT TCCCTCGTCG ATGGCCTAGC GGAAGAAGCG 
AATTGCTCGT GGTGCCCTCC TGGAAATGTC TGCACGGCAG AAGGCGTTGC CACAGAACTC 
AAACGGTGCC CGGCCGGGAG CTACTGCCCA GCTCCGGACT ACGTCCTTTC ACCTGCCACT 
GGAGGCGACG GCAGCAGTGG GGCTTTGAAG GTCACCTGCG CCTCCTCAAC TGGCGGCGTT 
CCCACCTGCG CCATTGACGC ACTTGAGTGT CCTCTTGGTT ACGTTTGCCC AGAGGGCAGC 
GCAACTGCCA AAAGATGCCC CCTAGGCCAG ACCGTCGCCG TGAGCGGCGC AGCGACCAAC 
TCCGGATGCA AGCCGTGTCC AGCGGGACAG TTCTGTTCTC CCTCAGGAGC CGTCGAGCCG 
TGTGCCGATG GCTTTCTCTG CATTTCTGGG GCGTATCGGC CGAAGCCGTT GACCGCGAAA 
GTCGGACGTC TGTGCCGGGC TGGACATTTC TGTCCGTCGG GCACCACCGT CGAAATCGCG 
TGTCTCCGGG GCACTCTCAG TGTCGGAGAA GGAAGTGGAA ACGCCTGCGA CGAGTGTCCG 
CCTGGGAATG TCTGCGATGC AGAAGGCATC AAGGCTCTAG ACGACCATCG GGCCTGTCCA 
GCGGGCTCTT ACTGCCCACC TGGTAGTGTC ACAGCGATTC CTTGCCCTGC TGGGACGTAC 
AGCGCCTTCA CATATCTCGC TTCCTCTCAG GAATGCCTAG CCTGTCCTGC AGGAAAGTAC 
TGTGAGAGTG CGGGAGCCCA CGCCGTGACT GGCCCCTGCG ACGCAGGCAC ATGGTGCAAG 
CGAAAATCGA CGAAGCAAAA TCACTCAGGT GGATACGCGC CATCGAGCCT CGTTGCCTGC 
CCTCAAGGTG CCTATTGCTT ACAGGCCGCA CCCATGCCTA TCCTGTGCCA ACCGGGGACC 
TACCAGGACG AGGAGGCGAC CGCCGACGCG TGCAAGCCGT GCGCTGCAGG CACCTTTTGT 
GGGTCTGCTG GCCTCACGAC GGAAGCAGGA AGCGGCCCTT GCCAAGCAGG CTTCTTCTGC 
AAGGAAGGCG CCAAGGTCGC CGCACCGAGA GACAAATCAA CTGGGGATAT CTGCAAAAAG 
GGCCATCATT GCCCGTCAGG ATCCAAGGCA CCTCAAGCGT GCCCCCCCGG GTACTACAGT 
GACGAGGAAG GTCTGGCCAC ATGCAAGCCA TGCCCTGCAG GCTCTCTCTG CCCTGGAGGA 
ACCAACGGGG AAATCGGGGG CTGCCCTGCT GGATCCTTCT GTAAGGCGGC TGTCTCGGCT 
GCTGCGGCAT GCCCCGATGG AACGACAAGC GAAGTGGAGA GTCTTGCGGA AGAAGGCCAG 
TGTGCCGACT GTCCAGCTGG AAAAGTGTGT GAGGCTGGCA AGCCGCCAGC CTCGTGTCCC 
GAGGGCTTCC TTTGTCTTGC GGGAGTCGGA CCCAAACACA TCGGAGCTTC GAACGCCGAT 
ATCGATGAAC TGTGGCTGGA CTCGACCTTC TGGGGTGGAC ATGCGATTCA CTACGGCGGG 
GCTTGCCCGC CTGGGCATTT TTGCCCCCGT GGGGCAACAG CCCCTGAGCC GTGTGCCCAA 
GGAACTGTTC GCGACCAAAC CGGAGGCAAA AGCCAGGCCG ACTGCACCAA GTGCCCCATC 
GGCAGTTTCT GCGATCCACG GATGCCGGTC CCTGTTTCCT GCCCCCCTGG ATACTACTGC 
CCCGAGGATA CCCAGGAGCC GTCTCCCTGT GAGGCTGGAT CTTATCTTAC CGCCAGCGGC 
CACTCCGAGA AGTCCGCCTG CACCATTTGC CCGGAGGGTT ATGCATGCAA TCGGGACGGC 
GTCGTAGACT ACAAGCTCTT CCCTTGCCCC ACAGGCTACT TTTGTGGCGC TGGAGTCCTG 
GAGCCTGAGG CGTGCCCCGC CGGAACTTAC GGGGCAGCTC CGAAGCTCAC CCGGGCAGAC 
CTGTGTGAAG CCTGTCCGCC TGGAATCGTT TGTACAACGC GCGATGAGGA GGACGGTTCA 
CGGCGACCAC AGGCGCTCGG CTGTCCAGCT GGTTATGTTT GCCCGAAAAG CACAAGTACG 
CCAACACTCT GTCCTGCCGG CACCTTCTGC CCCCGAGGCA CATCGACCCC CCGCACATGC 
CCGGCTGGCT ATTTTTGCCC GGAAGGAGCA GAAACTCCTG TCCTCTGTTC CAAAGGAACG 
TTCTGCTTAG AAGGCGCAAC TGAGCCCAGT GTGTGCCCTG CAGGCTATAA GGCAAAGGAC 
TCCTGTGTAG GCGCGTACTC CGTCGAGGCC TGTTGTGAGC CGTGCCCAGT GGCCACCTAC 
TCCGACAGGC CAGGAGCTAC ACACTGCAGC CCGTGTGCAG CAGGCTACAT ATGCAAGGGC 
GGTGCTACCA CGGCGAAACC CAGCGCAGAA GAGACGAGCT CTGAGGGATA CGTGTGTCCA 
CCGGGCTACT ACTGTCTGGA GGGAGCTTCG GAGCCTACCC CGTGCCCCGC CGGCACCTTC 
AGAGACCAGC CAGGAGGCGA GGCTCTCGAC GGCTGCTCTC CGTGTCCCCA CGGAACCTTC 
CAGAGGTTTC CTGGAATGGC GTCGTGCACC AAGTGCGGGG AGCATGCAGT CTCTGAGAAA 
TCCGTGGGTT CAACAACATG CACGTGCCTC GGCATCTCTC GGTTTTACCA AATCGAAGAC 
GGCACCTGTC CCTGCTACGC TCGCTCCTCC CCTCAAGGCG GAGAGCCTCA AGAGTCACAG 
GATGGCCGCA GTCCTTGTGA AGCGAAGCTA TACCCACGTT GTGCGTCGGG CAGCCACAGG 
GACGACTTTG GCGCCTGCGT GTTCGAGGCC GAAGTCGAGT GTGGCGAGTC ATGCCGTGGA 
GGCTCTGGAA GCTTTCTCGC TGCATTTGGT GTTTGCCAGT GCGCTGATGG ACCTCGCATT 
GCAGCCCAGT GTACATCCAC CTGCAAAGAG CAGCTGCCAC AGGTGTTCGT CTCAGATGCA 
GGCTTCGAAG AAATCGACTC TGTTCAGGGA TCCAAAACGG TCTACAGCGA GGGCCTTCCT 
CCCGACATCG ACCTCGCCCA TCTGCAGTTC TTGTGCAACA AACTCAAGGA GGGTCTGCGG 
GGCTTGCCGT TAACGTCGCT CTGCGGCGTC AGCTTTCATC AGGCTTCGTC TGCCGGTCTT 
GACGGGCTTC TGCGGATGCC TGAAGAATTT CGACCGGCGC ACGGTTCTCG AAATTATGAA 
GACTCTCAAG ACACTTTCGC TGGAACGCAA GTTCAGCTCC GGCGCTCCAT TTCTTCGGCT 
TCCTTCAAGA GGCCAGGGAG TCGCCAGCGA GCTACGGACC CGCGGCACGT TGTCCCCAGG 
CCAGTTCAGT GTCTCCAACG GGGGTCAACG ATCATCTGGG AACTCAAAGA TGGCGTCTAT 
CCGGTTTACC ACAAGGAGAG TCTGATGAAT ACGCATCCGG CAATGGATGA CGGCCCGTTT 
CGAGAACTTG CGCAAACTGC TCCGATCCTC CCGCAGTTCT TCGCCTACAC ATTTCTCGTC 
GCGGGCTCCT TCGTTTTTGC AGCCTCCGAC AATCCTCTGG AGATTGCCGT CGTTGTCGTT 
GTCCCCGACG GCGTCAGATG CCCAGCAGCA AGCACGTTCG CCGCTCCGCT GTCAACGCAA 
AGCCTGGGGA TTCTCGGCGT AACGACGGCA TCGCAGCGAC TCATGACAGA GCCCAACTGG 
GGTGTGGTGT TTCTGGGAGT TTTTGCTGTC GCTGCATGCT GTCTCCTCGC CTTCTACGCA 
GTCTGGAGGT GCAGACACAG CGCCTGGGAA GTGCGTGTCT GGACTGCGCC CTTGGGATCC 
GCGAACCGAA TCCCTAACGA CGACTTTAAG TCGGTTCTCG CCGCTTTGGA CGGTCTCCCC 
GGCGCAACGG GCAACCTGCG GTGCACAGAC ACTCGGCTAG TACACCACGT CGTCGTGAAA 
CCTGCTGAGC CACTGCTGGA ACCTACGGGA AAAGCAACAA GTCCATCCGA TGACGCGCCG 
ATCATTCCTC TCAAGGATGC GGCAGATGCG CTGGTTGTCG ACATCGAGTC TACCGACACA 
CGGCTTCTGC AGGTCATGTT CGAGAAGCTT CTTGATTTTG GCAATATCCT GAAGCGTCTC 
GTCGAGGATG AAGTGAAGGC AGTTAAAGCC GCCTGCGACG TCATGCCTCA AACGCTTGAA 
AGAATTCTAA GGCGGACTCG ACAAACGCTT CTGCTGCTGC CAAACGAGCA CATGTGGGCC 
ATCTCTCCGA GTCAGGCAAA ACTGCTGCAG GCCCTTGCAT GCGTCTTCAC CGAGGGCGAC 
AACTCTCCCG CCGTCGGAGA GGCGCTGGCC AACGCGCTGA CTCCTCATAC TTTGGAGAGT 
TCTCAGGTCT CAAAGGGAGG AGACCTTCCC GGTTCGCTTC TCGCCTGCGT CGACCTCGCT 
GGCGTCGCTG TCTTCGATGC CTTCGATTGC TTGTGGCTTC TCGAGCCTCA GCACGGCGAG 
GACTCGGTGG CCGACCGTGT GCTTGGAAAG CTCGAGAAGT TGTTGGAAGG CAACGACCCC 
CTGGAGGAGA ATTCCGACTC ACTCTTCGTT ATTTACGACA CCTTGTTTGC GGAGCGGGCT 
TATCAGGAAG AGCGCTTGCG GCGGGAGTGC ACGCGCGTCC AGTCGCGTTT TGCAGGGGTG 
ATGCAGAAGT TCAAAGACAC CTGCCAGACT CTTCACCTTC TGCCGGCGAA ACAGGCCCAG 
GCGTGGTTCG AGCTGAAAGA TTTCCAGCTG CGAGAGCTGC TGGACTTCGA AGCCAAAGCC 
TCCAGGAGCA TCGTCGAGGC CGCCGTGGGG GGAGTGCGGA GCGGAAAGGC GATGCTGAAT 
GACAACCTCT TGACGACGAC TCTGGAGATG GACTCCTCTA ACAGTCGCGC CTTTCTCTCG 
GGGATTCTGG CCTCTTCCCG GAAAACGCTT CTTGACCAGA TTCGCCAGCA ACTGGCAGCG 
GCCCTGAAGG AGATGAAGCA GCTGCTGAAG AGACAAGACG CCGCGTTGAT GGAGCTCCTC 
AGTGAGCACG CTCGAAGTTA CCGTACTGCA GCTGAGGCGC GACGCCACGC ATGCAGAGCG 
CTGCTCCAGC AGAAGCACAC AGAAGAAAAA CAGAGGCTGA AGCGGTGGAG TGCCACGATG 
GTCAAGGCGG TTAAGGAGAC CCGCAATGTG CCAGCAGAGA TGCAAGGGAA CGCGCGAGAG 
GAACAGGAAC GTCTGGATAT GGAGACAGCT TTGGAGCAAG TCCATCGTGC CACTGCTCAC 
AGACGACAGC TCGGCGAGGA TCGCCAACGC CTAGAAAATC AGATGGCTGA GGCCGCTTTC 
AGCACCGCGG TGGACCTACA CGAGGCGCAC CTCGACTTTC TTCGCGACCA GACCCGAATG 
GAGTACCAGC TTCTCGAGGC GTCCTCTAGG GAAGACCACG AGATCCACAG CGACTTCGCT 
GTCGAGTTGG GTACGCTGCA GCTGGAGGCG CTCGTGATGA ACGTGATGAA GAGAGCAGCA 
AGGCAAGAGG CAAACGACCA ATGCGAGACA ACTCTCGACG AAGTCCTGGA GACTGAGATG 
GAAGGCTGGA ACGGACAGAC AGTGGTGACA GCGCTCCAGG CTGTCCAGAG CAAAGTCACG 
AAGCAGCAGT CGGCTAGCCG CGAGATCAGC GCGAGTCTCG TTGCCAAGGG TAAACAAGCT 
TCTGAGGCTC TCACGCGCTG TGTGGCAAAC CGATACGATC TTATGACACA AGCAATCGAC 
CAAATTCAAG GAAAGTCATT CCAACTTGCT CTCAGCGCTT TGGAGGCGCG GGAAGACACG 
TGGAAAGCAG CTCTTTCCGC GGCTGGCAGA CTAGCACTGA ATGACGCGGA GCGACAGACG 
TCGGAGAAAG AAGTCCTGCG GCAACTCGAG GTTCAGAAAA CAGTCGAGGC AGACGACGAG 
TTTGAAAAAA CTCAACTTCT GCAGGAGTTA CTTGAGAAGA ACTGCCACAC TCGTCTACGG 
ACTGTCACGC AGCTACGCCG TGCCATGCTT CAGACGAGGT TGAAAGCGTG GGCGGCTGTG 
GCTCTTCATG GCGGATTCTT TGACAAAGTG AAGGCGCTCC AGAGCTGGAA AGAAGTGGCA 
GACCACCGGA GTCAAGAGGA CGACGCTCTT GCAGCAATTC GTGACCAGCG CCGACAGGCA 
ATGGGGAAGT ACGTGATGCG AGTTCGGAAA CTGGTGATTC GTGAGGAGAA GCGCAGAGTC 
CACCAGAAAG AATCCCTCGC TCACGCTGCC TTCGTAGTGA CCCAGCCTCG AACAGTGACA 
GGGAAGGCGC TGGGCACTGC TGGGGGGGTG CTGCAGGGTG CGGTGGAGAT GCTGGCGCAC 
AAGAAGCTTG ACGATGAATT TACGCGGAGG CGGAGTGCCC TGAAGGCAGA GCTCAGGCGA 
GAGTTGGAGG CCATTGACGA GGAGATAAAG CGACATCAGG AGATAATCGA AGCAGAGGCG 
GAAGACCTGT CAGGAGCCGA CGATGAGGTG CCTCAAGAAA CTGTGTCGAT GGGGGGATCC 
GTGTCGGAGC TCGGAAACGC TGAAAGAGGT CAATCCGTCC GCAAAAAGCG CAACAGCGAA 
ATGGAAAACC GTCTTAGTCT CCTGGATCGT CAACGCGCAG AGATTGTTAA CCTTTTCAAC 
GAAAACGAAC GCCAGCTCGA ATCCGAGTGG CGTACGCGGG CCGTCCATGT GCGGCATGAG 
ATCCTGCAGA AAATCACTGA CTTTGCTGTT GAGTTGGAGT CTAGTGGCCA GCAGCTGGAC 
AGCATAGCCG CTGAGGTTGC CAGAGATGTG ACCGTCCAGT ACCAAGAAGA ACTGAAAGAA 
CTGGAAGGAA CGCGGGATGC ACCTGCTGCC GAACAGGACA ACACCGATGG TTTCGAGGAA 
GACAACAAGG ATGCGGACGC TGCCCCTCCT GAAGGTGACC TAGACCAGAA ACTTCGGCAT 
CTGGCGATGG TGCGGTGCCA GAGGGTAATT TTGCGCCTAA AGCGGCTAGT GGAGAAAGGC 
AGCAAGATGG CTCGAAGCCT GTCTGAGGAG GCTTCTGCCG AGCTGAGACG TCTGCAGAAG 
GAGGAGAGGG AAAAGGAAGC CGAAAGGCGC GTCGAGCTTG AGCGGGAGCT GCAACAGCAC 
GAACGCGAGA AGGTCGAAGC AGAAAAACGC CTCGCGGAGT CCCTGGAGTC TCTGCAGAGA 
GAGTGGGAAG AGAGACTGGA GAGGACCCAA GAAGAGGGGC GGGCTCGGCT ACGGGAGCTG 
CAGGAGGCGG CGCGACGCGA AGCTGGACGG AAAGTAGGCG ACCAGAATCT CGGAGCGATA 
AAGCAGGAAA TCTTCGCGAG ACACCAGGAG GACGCCACGC GCTTTGATGA GGCCCTCGGT 
GCTGAGTATT GGAAGCAAAT GAATCGGCTG AGGGAGAGAC TGCAGGTGAA GATGATGGCG 
CGTAAGAGAC GCCTGGCGGC CGCAGCGCAG GAACAACTGA GTATAGAAGG GGACCGTTTG 
AAGGAAGCGA GGAACGCCTT CGTTGCACAG CAAGAATCCA TCAGTTTTTA CGATCCTGAC 
AAACAACGAG AGGTCGTCTT GCGAATCGGG CGGGCCTGGC GAGATGAAGC TCGCCGACGG 
ACAGCACAGA AGAAAGCAAA TGCAGAAGCA GATCAAATTG CCACAGAGGA CGCCGAACCC 
CAGGCAGACT TGCCGCCTCT TCAGAAACTA TCAACGTCGG TGCTCAACAA ACGCCTTGCA 
GGAATGCGTC GTGCGATTGA GGACACCATC GCTGAGATGC ACGGTACCAT GCAGTCGATC 
TCAACGACAG ATGGTCGGCG AAGCACATCG AGCCGGCCCA CTATTCACCA ATTTGAGAGT 
CTTGTTGATG CAACGGGTCA AATAAGTCAG CTACTTCAAA ACCTGGAAAA ACTGAGCGGC 
GTGTACTCTT CTATTATGCA CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_253930 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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