TGME49_223725 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_223725
Entrez Gene ID	7898233
Full Name	hypothetical protein
Synonyms	TGME49_023720,TGME49_023730,TGME49_023740
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018780014.1	XP_018635908.1	hypothetical protein

Pathways from BioSystems
Gene Ontology

The following TGME49_223725 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_223725 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi11048	XM_018780014.1 Latest version!	Toxoplasma gondii ME49 hypothetical protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_223725 ( XM_018780014.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018635908.1 Toxoplasma gondii ME49 hypothetical protein mRNA.

Price & Availability↑

CloneID	OTi11048
Clone ID Related Accession (Same CDS sequence)	XM_018780014.1
Accession Version	XM_018780014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031478).

ATGGAGCGCT GGGGGTCTGC CCCTCCAACA CAGCATGGAG GGCAGAGAGC GCTGCCACGA 
CAGGATCGCT CGTCGCCTCC GTTGCGCAAA GACGTAAAGG GAGTTTCGCC GCCCATCCCG 
GTCGTTCCCT TTTCTTCTCA ATCTGGAAGC CGATCTCTTG GGTCTCCACA AGGAAATTAC 
ATGCCGTATC GGCACGAAGC AGCTTCTGAG TGTTCGGGGA AAAAAGGGTC TCATAGAATA 
CCGTCACGGA GTGTGTCCAG ATCGGGTTCT CCATCCACTG GAATGGACGC CAAGCCACTA 
TCGGTTCCAC GGCCTTTCCA TCGTTCCTGG TCGACGAATC CTCAACCAGG AAACGGCCAT 
TCTTCGCATG TTTCAGGAAG CAGTCGCCTC TCCCGGTCCT TAACAAGCCA CACACCGTTC 
CACAACGCTC CCAACACCGT CCTTCTTGTC GGAGGAAACT CTGCGAGCCA GCGAAGGCGA 
CTTACTGACG GAACTTATGT CCCGATTGTG GCGTTAACTG CTCCACCCTC AGAAGACCGA 
ACGGGAAAGC AGTGTTCGTC ATCACCTGCT CGGCAGCACT CGTCTGGGAG CCGTGCTAGC 
AGCGCTAACC ACCACTCCAC ACGTCACAGC TCTTCTCGGG TTATTCAGAA ATCGCTTTCT 
CGCAGCCGTA GTGTGTCCCA CCACCGCAGC CTGCAGAATT CTCATGTAGA TATCCAAAAG 
TCTCACAGTA ACGACCACCA CTCTCACGGC AGCGGCCGCC TTTCTCACAG TTCGCACCAC 
AGTCGGCACT CAAAGTACCG CCACGGGCAT CACCGGAGCT CTCGGAACCA CTCGCCTTCG 
CCTTCCCATC ACTCTCGACA CCGTGACAGT TCCAATCCCA GTCATTCTCG CGGAAAATTC 
GCGTCGCCAT CGAGGAACTA TGGTTCCAGA ACGTCGCATC GGCCGCATTC GCGGGACAGG 
AAGATTGTCG AGAATCCCCG AACTTCTCAT GACAGCCGTA CCGGATCCAC GCTTCCCGAT 
TTTTCTTTGT CGCCTTCTCG ATATGCCCCG CCTATCTCGC CGCCTCGAAC CCATCAAACG 
TCCTATGAGT TGGACAAAAC TGCGCCACCA TACCACCCCG TTGCCCACTA CAACTACAGC 
GAGCGGGATT TTTCTCCAAG ACATCAGTCG CCGCGTTCCG GCTTCCAGCC GTCTCACGCC 
ATTGAAAATA AGTATGGAAG AGGGACATCG CGTGTAGATG TTTTAGACAG ACTCCACTGC 
AATGCTCGAG TGAGTGAGAC CGACTCTTAC CGACCAGGAA TTTCGATAAT CGGAGGAAAG 
CAACTCAAAC CTATTCGTGC CCGTTCTCCG CGCCGTTCAC GCTGCTACGC AGCCAACGTC 
GACTCTGATG GACGGTTTTC GGCATTCTAC CAGTCCAATG CTCAACGTAC GATTTCTCCT 
AGCGATACCT GCCGTCCACG GGTAGTAGCA CGTCCACCAC CGTCGACTTC AAGATGCAGT 
CCGACCTGTG CACAATCGCG CCGTTTCGCT CCTCTGGATT ATTCAACTTC AAGCGCGACT 
CGTCCTCTGC GAAATGTCCC AGTGTACTTG CCTTCTCGGA GGAATTCGCC TTCCCGGCGT 
CCTCCTTCCC AAGAAGGCCG AACCCACTGC AGTCCAGTAG GCGGTCGATC GGGATGCAGA 
GCAGATCCCT CCTTGCGCGA CTGTTGCTCA GACATCCCTC CACGGGGCCA GTGGCTCTCA 
GGGAAGACAA GAACAAAACT CTCTGATTTT TGCCGTTCTG GTCGCCTTAC CCCGGTGACG 
GGATGCGCAA AAGCTGCACA GGAGGACTCG TTTTTTGGCA GCCTTCCCAA TTCTTCTTGC 
CAAGGAGAGA TGCACTCCTT CCGTCGACCG TTCCGAGCCA GCTCCTCGGA GCACTGTCCA 
CTTTCAGGGC TCCGTACACC GAGTCCGCTG TTAACTAGTG CGCACTGCCA TGGAGCTATT 
AATATGTCTT GTTCCTCTTT GCCGTCTGTC TGCGGGGGGT CTGCTCTTCC CGTGGATCTC 
TCACGTCGCT CGCCGCACTG TCACCCCGTG AGCACCATTC TGGGGGACTG CGTGACGCAA 
CGGAAGTCTG CGGCATGCCG GACAAATTCG CCCGCTGGCA GATGTTTCGA CAGCTTGGCG 
ACACAGTATC CACCTCCTCC TTGTCTCAAA CTTCACCGCC TTACCCACGA CGAGAGAAAC 
CATCTTCCGC GCGACAATCT GCCTCTGTCG CCTGGCCGAT GCATTCTTCA AGAACCCACG 
GATGAAGTGG AAGCGAGACG GGCTATCGAT ACGGCCGACC AGCTCCTGAA AAACGCGTCG 
CCCAGCGCAC GGGTTCGTGG GAATCTGAGT TATGAACAAG GCTTGTCGTC AGTTTCGTAC 
AAAAGGGAAG CGGATTCTTT GGGCAGGCGC TCCCCTTCAC TAGGAAGGCG GGACTTTGCC 
AGCTCGGCAG CCGCCTCTAC GCCTTTCCTG GAAAGCAAGC GTAGTGGGTG TTCTCGGGAG 
TTTTGTGAGT CGAATTTTCA GGTGTTCTCT AGCGCGCCGT ATCCCGAGCT CACAAGTGAA 
CGAAGTATCT GCTCTTCGCA GTCGCTCTCG AGCTCGAGAC GAAGACCACT GTGCTCTCGT 
CGACCCGACA CGGAAACGAC TCTTTTGCGC GCTTTGAAAT CCGGCCCATC TTCTCTCCCG 
GCCTCGGGTT CTTCATCGAC TTCTACCGCC CCGTTTCTGT CCTCTGTACC GGGAAGAATC 
GACAAAGGAA GCTGTCTCGG CCGTGTCCCG CTTGAGGGTA CCGGCCTGCG GTGCAGTCAC 
CACTGTGACT GTCTAGAGAC CCGCGACGAG TCGTGTCTTC CGAAGCGGAA AAGCATGGAG 
ACTTCCTCGT TCCTGTCTGT CTCGCACGAC AAGCCACGGC TTCCTGCTCG GGTCTCAGGC 
GAAATTGCCG CCGAGCGCCT GCGCCATAGA CTGGATCAAT GCCAGCGGGT GGAGGAGTGT 
AAAGACGGAC TTCCCGCCGG TCGCCGTGAC TCCGGCGGGT GCATCGACAG CGGCATACAT 
GCTGACACCT GCCACGGCGT TCATGCGGCC CGCCGGTCAC CAATTTCGCC GCTCTTCGAA 
GCGAGCCGAT GCACTCACCT TTCAGAGGTC GAAGCCATTG AGGCTGAAGT GGAATTCGTT 
GAGAAGAGGC GCCGCGCCCG CGAGAGGGGC CAAGAAGACA AGCGTACGGA GACCCTTCTG 
CGCTCCTTCA CCTGCCTCCC CCGTCTGTCC CTTCAAAACG CGAAGCGTAT CGACACTCAA 
CAGAAAGCTC TTCTAACCGC ACGATCGCGT TTTCCCGCCA AGTGTTGCTT TCCAGGGGAT 
TCTGAGGCCG ACGCCGCAGC ATGCAGCGAT CTGTCCGAAA GCCACACCCA GAACCTCGGT 
TCTGCCCTTG CGTGTGCCCA CCCGCCTAGC CCCCACACCC GCCAGCCCAG CATTCGCCTC 
GAGTCTCCGT CGGGTCTTTA CCTAGGCAGA TCGAGTGGCA GGCGCATAGC GGAAAGCAGC 
TGTCCGTCGT CTCTGGGGAC CCCGAAGAGC AATTTCGAGA CTTCAGTGTC TCTCTCTTCG 
GGCGCCTCGC GAGCGCAGCG TGTAGCTGAG ATCGTCGAGC GCCTGAGTCG AGTGCAAGAA 
GCGGAGTGCG CGAATCTGAG AAGACGCTCG GCGGAACGCG CCGCCCGGAG TCGTCTGACG 
CCTCCAGGTA TGAACATGCC GACCCTTGAA GGCTGCCGGC GGGCCAACGC GTGCACGGAA 
GAGATAAAGA GTTTGCAAGA AGCCTCTGCG CCTGAGGGCG CCTTCCCAGT CTGCCCCTTG 
CACGGTGGAG GCGCAAAAAA TCCGAGAATA AGAGACGAAG AGTGGAAGCG AGACCAGAAA 
CCTAGAGGGA CCGCACTGAA GCTGAACGCC GAGCAACGCT CGCAACTCGC AGCGGCCTGT 
GAGACCCGGA ACGCCCTCCT TGCGGCTCTA GCCTCACGAG CGCGACGCAG AAGAGAAGAA 
GCGACCGCGA GAAAGCGTCG CGATGGGGGA AGACACGCTG GAAGAACAGA TGGGTTCCAA 
AGACACCGGC ACGACGGTAG GGGCGAGAGG CACCTCTCGC CTTCTCTAGG GTCGCCTCTC 
AGCTCGCGAC CCGCTGCTTC TAGCGTTCCC AACTTGTCCA TTTCGTCAAA GTCGTGTCCT 
TCCTCTTCTT GTTCTGTCCA GACGGCCTCC TCAATCGCTT CCCGTCCGCC GTCGTCGATC 
CACTCTGGCA GTCGCGCCTC CTCCCTTTCC GCCGCTTCTC CCTCCCTACC GTCGCCCATC 
TCCCCTCACT CCGAGGAAAG ACACCCGAAG CGTTCAGGAG ACCTGAAGAG TTCAGAAGGT 
CAGAAGAGTT CAAGAGATCT GGTGTCTGTA CAGCTCTTGC AAGTCCCTTC CGCGAAAGGC 
TCCAAACCGC CGCGTCGCTC AGGCTCCAAC GCTTCAGCCC CAGACCAACC GGCCTCTCCT 
CGGCGCTCGG CTTCTCCAGT AGAATCTCCG AGAGACCTTG CCCCTCCCTC AAGAAGTCTC 
AGTCGTGCCT CTTCGGCGGC CAACTCGAGA AGCGACGGGA GTGTCTCTAC TAGCAGGCTT 
GCGGAGATAA AAAAAGCGAA CGAGGGCGAC AAGAAACAGG AGAGCAAGGG GGGGAAGAAG 
GAGGTGAAAG AAGAAAAAAA GAAAGAGAAG GCGGCGCGGA CGCTGTCGCC CTCGCCGACT 
GTGCAGAGCA TCAGCTCCCG CGCGTCTTCA ACGCGAAGCA AAGGGAATCC GGTGAAGCTT 
TCGTCTTCCG CTGACGCGCC ACACGCGCAG AGGAAAGAGA AAGGCAAAGT GGGGGGGAAG 
AGGCCGACGA AAGAAGTCTG GGACAAGACA AGAGACCAGA CCGCCCTCTC GCCGCTTCCT 
AGCGCGAGTG TGAGCAGAGC GTCGTCGACC GCGAGTGTCT CCCGAGGCCA CCACACGGCT 
CGCTCTAGAA ACAAAAATCT GGGCAAGACA GCGAAGAAAG AGAAACATAC AAAGTCTGTC 
AAGGCCCTGT CCCCGTCTCC CAGCAGGAGC CTGAGTCGCG CCGCTTCGCA TTCGAGCACG 
CGCAGCCACC ACAGGAGACT CTTGGGGAAG CCTGAGTCCA CCCAGGAGGG AGAGAAGAGA 
GCGAAGACGC ACAAGACCCA CACAAGCAGT TCTTCCTCAA CTTGCGTGAA GGGAAAGAAG 
GCGAGGGAGG CGCCTCCTGG AGAGCCGAAC AAAGAACTGA AGCGAGGCGC CTCGGCCAAC 
CCCTTGGTCG GCATGACTTC CCCTAAGCGC ATACACTCGA AGAAACCGCT GTCGCTGTCG 
CCCTCTCCGT CTCGGCAACA GCGCCATAAC GCGTCCCTGA AGAAGCAAAA ATCATCGTCT 
GCTCACGAGA GACACTCCTA TCGGCGCTCT GTGACGGCCT CAACTTTGCC GAGCCCTGAG 
GCTCTGGCAA GGGGAAGACG TATCTCCCGG GGTGGGTCTG CCCAGCGTGA GCAGGCACAA 
CAGAAAGAGA AACGCCGAGA CGAAGACGCC CGGAAACGCC CCCACGGATC CCAGCGACTG 
CCAAGCCAAC TGCACCACTC TCATGACAAG AAAAGCAAAA CACACAAAAG TGCCTCACCT 
TCAGTCTCCC CTTCAGCTGC TCATTCTCGC TCTAACTCGC ACTTGCCCTC TCGGTCTCCT 
TCTCATTCTC GTCTAGCTTC TGCTTCCTAT CTCTCTCCTT CGCGCGCCTC GTCCAGGCGA 
CACGGGCGAG AACACAGTTC GAGTTCGGTG GGTTCCAAGA CGCACAAGAC GAAAGATTCC 
GAGAGACACA AGAGGACGAA GGACGATCCG ACAAGCTCGC CACCAAGTCG CAAGGACCCG 
TCGCAAGCGG GTGGAAAAGA CGCGTCGCAT GGCCAGCATT GCCTGGCGGC GTACCCACTG 
GCTGCGCACT TGCCTCTGAC GCCTCGCGAG GTTCTGCCTT TTTCAGAGTG CTTCCTAGGG 
GAGGGGAAAC CGCGGGGCAA AGCTCCTGAA GCGTCTTCCC CCTCCACGCA GTTCTTCGCC 
TCTCTCGCGG ACCTGCTTTC GCCGACTTCT CGCGGAGCTC CTGATGGACG AGAACGGTCA 
CCAGCCTCTG CGGACGTCGT GCAAGAAACT CTGGCGAAAG CTGGACTTCT CTGCGGGTCT 
TTCGCTTCCC TCTTCTCCCC TGGATCTCTT GTCTCACAGG AAGCAGGACT CAAGCGGTGG 
AAGGAGGAAA CGAGGAACGA GAGCAACTCG GCACTGGCTG CGGGGCCGGA GAGTAATGGA 
AGAGGCGAGG CGACTCAGAC AGCCGACGAG GCTCCTGTAC ATCCGACAGC GCTCGTGGAT 
TCGCTGTTGA GGGAAATGAG CTCGAAAGTG GAAGGATCTT CTGTTTCGCG TTCTCTTCTG 
CTGACGTCGG AGGTTCGGCT ACGCGATCTC ATCGATGGCC AAACAACACA GGCATGCACC 
CCAGGGGGCT CGGTTGCGAC TTCCCAAGCT ACCTCCTTGT CTTCCTCTTT GCAGTTTGCA 
GACGCAAGTG CTACCCTCTC GTTCATTGCC GAGGAACTCG AGGCGACGTC TCAGCTATGC 
AGGAGACTCT CCGGAATGCC CCAGTCCCCT TCTACCTCAG CGTCCTCCAC TTCCTTCTTG 
TCTTCCTCCC TTTCATCCTC TTCTTCTTCC CTTTCGCCTC CTTTTTCGTT GCAGGACGTT 
CCTTCATCTG CAACGACGGC TCACAGCAGT GACTCTTCGC GAGGTGCAGG CTGCGGCCGC 
GGCTCATGTT CTGCTTCTCC CTCGTTGGTT TTCTCTTCTT GTCTCTCTGT TCCCTCTCTT 
GTCTCTGGGG GAGCCGACGG TGCTCAAGAT GCCGCGCTTT GCTCCGTGGC CGGGGCGGGT 
GAGGAGCTCT TCACGGGGCA GGAGACAGAA GAGACTTACA GAGAGAGAAA TGGGTGCAGT 
GAGCGGAACC AAGAAAATGA GGCCCGAGTA GACACAGATT CACCGACACC CGGAAACAGA 
GAGTTTTCAG ATAAAGCAGA CACCCCCAAA AATACACAAA ACCTTCGCTG CACCACGTGC 
CGTGCCTCTT CGTTTTCGTC TTATTCTTCC TCGTCGCTCT CTTCTTCGTC AACTCCCTGC 
TCTTCGTCTT CCTCGTCTCC CCGTTCTTCT TCTTCCTCGT CGCTCTCTTC TTCATGTTCT 
TCTTCGCTGG CGTTGTCTTC GTCCTCTTCC TCCACTTCTT CTTCCTCGTC GCTCTCTTCT 
TCATGTTCTT CTTCGCTGGC GTCGTCTTCG TCCTCTTCCT CCACTTCTTC TTCCTCGTCG 
CTCTCTTCTT CATGTTCTTC TTCGCTGGCG TCGTCTTCGT CCTCTTCCTC CACTTCTTCT 
TCCTCGTCGC TCTCTTCTTC ATGTTCTTCT TCGCTGGCGT CGTCTTCGTC CTCTTCCTCC 
ACTTCTTCTT CATCCTCGCC TTCTCGTTCT CGCGTGAGTC GCGAGACACA AGGAAACACA 
CGAGAGGATG AGCACAGCAT TTTGGATCTG TCCGAACTGC ATGCAGAACA GGACGCTGAA 
TCTGTCCGCG CTTTGAGCGA CTCGGCTTCC GAGTCGCTGT CTCCGTTTCT CTCGACTGCG 
GCTGAAATTC GTATGTTGCT TCCGGACAAA GAAGAGGTTG CCGCGCTCTT CTCCTTCGTT 
TCTTGGAAAA GCTGCTTTTC AGCTGATCCG AGCAAGCGCA GACGGTCGCG CAGAAACTCC 
AAAAGAGGCG CCGAGCCGTT GACGAGCGCA TGGCGTATAG GGGCACCCCA GAGCCTCTTG 
GGAGCAGCTA GCCACTTGGG GACAAGACCC TCTCTTGCCG AGGTTGCCGC AGGTTCCTTC 
TCTATGGTGC AGAACGGGGG CGACCAGGGA GACGAAGACG CGGGAGACAG ACAGACAGGG 
GACGGACACA GCGAGGAAGA AACAGAGACC GAAGACGAAG AGGTGGAGGA CAGCACAGGA 
CAGCGGAGGA AGCCGGCATC TACGAAGTTG AGAGATTTGC TGAGAGCCTT CAGAGACAAA 
CCTGGTGAAG CGGCGCACCC AGCGGCCGAA GAGACAGAGA CAGAGGAGGT CGACGAAGAA 
GAAAGGCAAG CGCTTCTACA GAGACAGGGG GCGAGCGAGA GAGGAGACAC GGCGGACGAC 
GAAGCCGCAC AAGCAGAGGC CCCCAGTTGC CTGGACGAGG CGGCCATAGA AGACCTTTGG 
CTGCTTCAAC AAACGAACGG GGAAACAAGG AAACTGCTGG ATAACGTATT CCATTGTTTG 
CAGCCCGTGA CTTCTGCCGC TTTCGACGGA GGGGAGGCGG CGTCTTCACC TCCGACTTTC 
TGGCGCCCCA GAGGCGCTGC TATGTCGCCT CGACGGTGCT TTCTCCCCTT GGTTTGCGAG 
CGAAGACAAG CAGAAGTCGA AGGTGGAGAG ACTGAAGAGG GCGCACTCGA GCGGCAAGAA 
TTGGCGCAAC GCGTTCAAAC CTTGCGCTCT GCCTTCACTG GGGCAGTCCC CTCTTTCTTC 
GCCGACTGTC GCTTTTTCTC TGCCAGTACA CCCCGCAGAT GTCCGACGGC AAGGCGCAGA 
GCCTTTTCCT CGGCTGGCTC GCCCCCCTGC CGCAGGGGAG ACGGAGACGG CGAGACAGGA 
GAAATCCAAC CGAGAAGGAG GACCTTGAGG CAGCTTCCCC AGAGAGAGGA GAAAGCAGCA 
ACCGGGCCAG CTTCGCCTCT GGCGCGCGCT TTGCCTTCTT CTGCACGTCG CCGAACAGAA 
GCGAGAAATC CCGAGGAAAC CCAAACGAAG GAGGAAGAAC ATGGGGAGGC AGGGAAGGTG 
GAAGAGCCTC TTTTCTGTGC AGAAGGTGGA GAAGAAGAGG AACGAGGGGG GGGAGCGAAG 
AAGCTGAAAG AGAGAGAGAC GAAGCAAGAG GAAGAGACGA GAGAGAGACC AGAAGAAGTG 
GAGGAAGCTA CGGTACTTGA ATCGAGTAGA GAGAGTCGCT CTGCCTTAGG CTGTCACTGC 
TTTGCATCGG CCTTTAGAAC GTCGGCCTCA TTGCCCCCTT TCGCTTCTGC CAGTAGCTCT 
GAGAACGCCT TGGGTAAGAG CGAATTGAAG GAGGACGCGG TAGCTTTGCA AATCGACGAC 
AGTCCCAAGG CGTCGGAATC CGGAGAAGGA AGGAGCGTTG AAGTCCCCGA ACCGGCAGAC 
GAGGGACGAA TGGCTTCAGG AACTCAAAAC GGTCCATGTC AGAATGAAGA AGAAGTGTAT 
GAGAAGAAAC CGACAGAACC AGGGACAGGA ATAAACGAGC AAGAGCATGC GCCAGCACCT 
CGTTCTTCCG CCCTCACGGC TGTCCATACT CCCGACGACA GACCCGTGGG CAAGGAAACA 
GGAGACAGAT ACGAACCCCT GGGGGAGAGG CAGACAGGCC ATGGAGAAGA CGCGAAAGGG 
GAGAGCGATC CAGATAAGAA ACAAGGCGCT CTGCAGCCTT CACTGTTGCA GGGGGAAACG 
CGAGTATCTG AAGAGCGAAG CAAACCCCCA CCATTGTTGT CAGAAGATTC ACAAGAAACA 
GAGGAAGAAC AGGAGTCAAT CATGGGGAGT TTCACCCTTC AAGAACTCTC TTCTTCGATC 
TCCGGCAAGC GTCCCTTTCG GCAGTCGGGT TTGAAGACAC CTGAGGCGGA CCCTCTCGCA 
TCGCCAGCTT GCTCTGAATT TACATTTGCC ATCGACTTTT CTCCGCAGCC GTCTGAACGT 
GCAGGCGCAG CTCACACCTC TGCGCCTGAG GCTTCTTTCT ACTCCAACTT ATCTTCGGAG 
ACACGACAGA GTCCAGAAAC TGAGTCTGTG TCAAAGGGGG TTCCTCAGAT GAAGTTAACC 
TTGCCCAGGT CTAGGGCGCC GTCTTCTTCT CTGTCGTCTT CTTCCTCTTC TGCCTCGTCT 
GTGTGCGTTT CTTCTTCCTC TTCTGCATCT TCCTGCTCCA CCTCGTCTAT GATTCTGTCT 
TTCTCGTCTT CTTCGTCTGT CCCGTCTCTC TCTTGGGGTC CTTCTTCCTC TACTCCTTCC 
TCTTCTTCTT TCGGGTGCGA CGAGAGTGAC CTACCGGCTC AGTTTGCCTC TTCTGCTTCG 
ACCGTCAAGG ACAGACTGCT ATGCTTAAGT GGGCATCAGA ACGTGGAGAG GGCTGCGAGC 
GAGACGGGAA GAGAAGAAAC GGCGACAAAG ACAAGCGACG AGACTCTGGA CGCCGCTGGA 
GCACTCGGAG ACACACACAC AAAAGAATCT GCATGTGACG TTGTCGCGAA TGGGCATGTC 
AACAAGAGGC TGGCCGACGC TGGCGACGAA GCTCCTTCGT GCCCACCCCA CAGCGAGGTC 
CGCGATGGAG ACAGTGCGGG CAAGGCGCGC GAGCCTCCAC CCGAGAGGCT TCTCACCAGC 
GACTCCGCCG AGACAGCGTC GCACATGTGG CTTGGCTCTT CAGTCTGCAT TTGGGAAGCA 
AACGAAGGAG AGAAATGCCA AGACACTTCA GCGGAGCCAC AGACTCGAAC TTGCGCCATC 
CAAGCAACAG AAGAAGGCAT GTACACGCTG TCGCCTCTTT GTGCGACGGG TCCTGGAGGT 
CTTCTGTACG TCGCCACAGA AGTACCAGCT GGACAACGGG AGGTGGAAGT ACACCCCAGA 
GCTCTTCTTC GCTCTCCTCT CGGCTCTCAG GCTGGCGGCT GGGGCCGTAC GAGGAGTCGC 
CGAGGCGGAG CCGAACGGAG GCACGCGCCC GGGCGGGAGA GACACCCAGC AAAAGAAGCT 
GTCACGACCC GGGCAAAAAT CGAGGAATGG AGGCATGCAG GGGACATCGA GAAGTTTAGA 
AAAGTCTGTT TCCTGGGACG TTCGAACTCT GCGTCTGACT CTAAAACGTA CGTTGTCGAA 
AAGCCTCTCG CCCATCCTGG TCCCTCTCTG GTAGCTTCCC ATGAGGAAGC GAGGGAGAGT 
GGGGAACCAG CAAAGAATGA GAGAAAAGGT AGAACTCGAC ATCCAGGTGA GAGAAAAGAA 
GGCTCAAATA GCATGCTGGA TCGACACAAA CTGGGTTCCG AAGAGACGCA GGAGACACCT 
GGATCTGTCC GTTCCTCCGG ACTAAAGCGG CCTTCTCTCG CGCGCCTCAG GGGGCTGCCA 
GAACTTCTGT TGAGACACGC GACGCATTTT TCTCGGAGCC CGAGGGTGGT TGGGAACGAC 
GGTTCGCGAG ACCAGACGGA GTTATCAAAC GAAGAAATAA CAAAGAGCCG TGAGAGGGAA 
CGTTGCGGTT CTCGTTCAAC GCGGATTCAG ACGCTTCTTT CTCAGTCCGT TTTGTCCCCC 
AAAGAGGCCA GAAATCTGCG ACAGCGAGAG AGGAAAAGTT CTCGCGAGCG ACAGGGTGCA 
GAACGCTTAT GCAGGAGGAG CGAACGCGAA GGTTCAGCAT CCAGGCCCTC GTCGCCTGCC 
TCTCTCCGTC AGCCTTCGCG TATCTCTGCA GGTTCATCTT CTCGTCCTGC TTCCTCTTCT 
CCCTCGGCGC CTTCGTCCGT CTGTCCCCAC AAACCTGCAT CAGGACTTCG CCAGAGAGGT 
CCTCGCCGTG CAGGCAGCGA TGTCTCAGCA GAGAGAAGGG AACAGAACAT GCCAGAGGAG 
AAGCGCGGCG ACGGAGGTCA CTGTCGTCTC TCCCTGGCGT CTGGGCGCGA GCCTCAAAGG 
GTCTCTCTTC CAAATATGGC CTTCCAAAGA AGACCCATCA TGCGCGCGGG CGTCTCACCG 
GTCCCCTCCA AAACTCCAAT CATCTCTCCC GCTTCGCTTC CTTCGTCGTT CCAAGTTTTC 
TCGACGCCGC GGAACTTGTG GTGTTTGTCA GACTCCACGC CTTGCCGCGC TTTACGTTCC 
ACGACAACGC CTGAGTCTTT TGCTGGCTCT TCCGTTTCTT CTCCCGTCGA GGTTCATTCA 
CTCGCTTCTT CGTCTTTGTC TTCCCCGCCT TGCGAGCGTC TTCCCGACGA CCCAGTCGAC 
AGCATGCGCC TCGTGAAGGC CAAACTTCGA CTCGCCCGAG CGGCGCAACG CGGCCCTGTC 
TCGTCCTTGG TCTCGGCTTC TCCGGGGAAA CTGAACGAGA AAGGCCGCGC GTTTCCTTGT 
CGCAGCGCTG CCTCAGAGGG ACATGGAGAC GACTCACAGA GGTTCTTTCA GTTCCTGCGC 
AAGACCTTTG AAGCAGGAGG AGAAGGTAAG TATGCGTCCG TTCTCCGGCC CCTCACGTCT 
GCAGCTCGTC CCTCGTTCGT GTCTGGCTCT TCACCGTTCT CTCCCTTGTT CGCGTGTCCT 
GCGTTTCGTC AGCCACCGGC TCCTAAAGCT GTGACATTGA CTCCAGAAGA CAAGACAGCC 
CGCTACAGCA GATGCTTTTC CTCGTCTGCG ACTCTCGAAG ACCAACGCGT CGCAGCCATC 
CCTTTACCCC ACTGTTCACT GACGCCTGCC AGTCTTCTCC GTAACTCCAG AGGTGCCTTT 
TCTGCCCTCG AGTTCCAGCG TTTGCCGACT GCCTCGTGTT CCGGCCTTGC CGGCGAGGTC 
CTTGCAGCGG AGTGGGCGAC GCGACAAAAA GGCGAAGGAG ACAAACGGGC TCTTTTCAGA 
GACGAGAAGC GCGCGCGTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018635908.1
CDS	288..12851
Translation	MERWGSAPPTQHGGQRALPRQDRSSPPLRKDVKGVSPPIPVVPFSSQSGSRSLGSPQGNYMPYRHEAASECSGKKGSHRIPSRSVSRSGSPSTGMDAKPLSVPRPFHRSWSTNPQPGNGHSSHVSGSSRLSRSLTSHTPFHNAPNTVLLVGGNSASQRRRLTDGTYVPIVALTAPPSEDRTGKQCSSSPARQHSSGSRASSANHHSTRHSSSRVIQKSLSRSRSVSHHRSLQNSHVDIQKSHSNDHHSHGSGRLSHSSHHSRHSKYRHGHHRSSRNHSPSPSHHSRHRDSSNPSHSRGKFASPSRNYGSRTSHRPHSRDRKIVENPRTSHDSRTGSTLPDFSLSPSRYAPPISPPRTHQTSYELDKTAPPYHPVAHYNYSERDFSPRHQSPRSGFQPSHAIENKYGRGTSRVDVLDRLHCNARVSETDSYRPGISIIGGKQLKPIRARSPRRSRCYAANVDSDGRFSAFYQSNAQRTISPSDTCRPRVVARPPPSTSRCSPTCAQSRRFAPLDYSTSSATRPLRNVPVYLPSRRNSPSRRPPSQEGRTHCSPVGGRSGCRADPSLRDCCSDIPPRGQWLSGKTRTKLSDFCRSGRLTPVTGCAKAAQEDSFFGSLPNSSCQGEMHSFRRPFRASSSEHCPLSGLRTPSPLLTSAHCHGAINMSCSSLPSVCGGSALPVDLSRRSPHCHPVSTILGDCVTQRKSAACRTNSPAGRCFDSLATQYPPPPCLKLHRLTHDERNHLPRDNLPLSPGRCILQEPTDEVEARRAIDTADQLLKNASPSARVRGNLSYEQGLSSVSYKREADSLGRRSPSLGRRDFASSAAASTPFLESKRSGCSREFCESNFQVFSSAPYPELTSERSICSSQSLSSSRRRPLCSRRPDTETTLLRALKSGPSSLPASGSSSTSTAPFLSSVPGRIDKGSCLGRVPLEGTGLRCSHHCDCLETRDESCLPKRKSMETSSFLSVSHDKPRLPARVSGEIAAERLRHRLDQCQRVEECKDGLPAGRRDSGGCIDSGIHADTCHGVHAARRSPISPLFEASRCTHLSEVEAIEAEVEFVEKRRRARERGQEDKRTETLLRSFTCLPRLSLQNAKRIDTQQKALLTARSRFPAKCCFPGDSEADAAACSDLSESHTQNLGSALACAHPPSPHTRQPSIRLESPSGLYLGRSSGRRIAESSCPSSLGTPKSNFETSVSLSSGASRAQRVAEIVERLSRVQEAECANLRRRSAERAARSRLTPPGMNMPTLEGCRRANACTEEIKSLQEASAPEGAFPVCPLHGGGAKNPRIRDEEWKRDQKPRGTALKLNAEQRSQLAAACETRNALLAALASRARRRREEATARKRRDGGRHAGRTDGFQRHRHDGRGERHLSPSLGSPLSSRPAASSVPNLSISSKSCPSSSCSVQTASSIASRPPSSIHSGSRASSLSAASPSLPSPISPHSEERHPKRSGDLKSSEGQKSSRDLVSVQLLQVPSAKGSKPPRRSGSNASAPDQPASPRRSASPVESPRDLAPPSRSLSRASSAANSRSDGSVSTSRLAEIKKANEGDKKQESKGGKKEVKEEKKKEKAARTLSPSPTVQSISSRASSTRSKGNPVKLSSSADAPHAQRKEKGKVGGKRPTKEVWDKTRDQTALSPLPSASVSRASSTASVSRGHHTARSRNKNLGKTAKKEKHTKSVKALSPSPSRSLSRAASHSSTRSHHRRLLGKPESTQEGEKRAKTHKTHTSSSSSTCVKGKKAREAPPGEPNKELKRGASANPLVGMTSPKRIHSKKPLSLSPSPSRQQRHNASLKKQKSSSAHERHSYRRSVTASTLPSPEALARGRRISRGGSAQREQAQQKEKRRDEDARKRPHGSQRLPSQLHHSHDKKSKTHKSASPSVSPSAAHSRSNSHLPSRSPSHSRLASASYLSPSRASSRRHGREHSSSSVGSKTHKTKDSERHKRTKDDPTSSPPSRKDPSQAGGKDASHGQHCLAAYPLAAHLPLTPREVLPFSECFLGEGKPRGKAPEASSPSTQFFASLADLLSPTSRGAPDGRERSPASADVVQETLAKAGLLCGSFASLFSPGSLVSQEAGLKRWKEETRNESNSALAAGPESNGRGEATQTADEAPVHPTALVDSLLREMSSKVEGSSVSRSLLLTSEVRLRDLIDGQTTQACTPGGSVATSQATSLSSSLQFADASATLSFIAEELEATSQLCRRLSGMPQSPSTSASSTSFLSSSLSSSSSSLSPPFSLQDVPSSATTAHSSDSSRGAGCGRGSCSASPSLVFSSCLSVPSLVSGGADGAQDAALCSVAGAGEELFTGQETEETYRERNGCSERNQENEARVDTDSPTPGNREFSDKADTPKNTQNLRCTTCRASSFSSYSSSSLSSSSTPCSSSSSSPRSSSSSSLSSSCSSSLALSSSSSSTSSSSSLSSSCSSSLASSSSSSSTSSSSSLSSSCSSSLASSSSSSSTSSSSSLSSSCSSSLASSSSSSSTSSSSSPSRSRVSRETQGNTREDEHSILDLSELHAEQDAESVRALSDSASESLSPFLSTAAEIRMLLPDKEEVAALFSFVSWKSCFSADPSKRRRSRRNSKRGAEPLTSAWRIGAPQSLLGAASHLGTRPSLAEVAAGSFSMVQNGGDQGDEDAGDRQTGDGHSEEETETEDEEVEDSTGQRRKPASTKLRDLLRAFRDKPGEAAHPAAEETETEEVDEEERQALLQRQGASERGDTADDEAAQAEAPSCLDEAAIEDLWLLQQTNGETRKLLDNVFHCLQPVTSAAFDGGEAASSPPTFWRPRGAAMSPRRCFLPLVCERRQAEVEGGETEEGALERQELAQRVQTLRSAFTGAVPSFFADCRFFSASTPRRCPTARRRAFSSAGSPPCRRGDGDGETGEIQPRRRTLRQLPQREEKAATGPASPLARALPSSARRRTEARNPEETQTKEEEHGEAGKVEEPLFCAEGGEEEERGGGAKKLKERETKQEEETRERPEEVEEATVLESSRESRSALGCHCFASAFRTSASLPPFASASSSENALGKSELKEDAVALQIDDSPKASESGEGRSVEVPEPADEGRMASGTQNGPCQNEEEVYEKKPTEPGTGINEQEHAPAPRSSALTAVHTPDDRPVGKETGDRYEPLGERQTGHGEDAKGESDPDKKQGALQPSLLQGETRVSEERSKPPPLLSEDSQETEEEQESIMGSFTLQELSSSISGKRPFRQSGLKTPEADPLASPACSEFTFAIDFSPQPSERAGAAHTSAPEASFYSNLSSETRQSPETESVSKGVPQMKLTLPRSRAPSSSLSSSSSSASSVCVSSSSSASSCSTSSMILSFSSSSSVPSLSWGPSSSTPSSSSFGCDESDLPAQFASSASTVKDRLLCLSGHQNVERAASETGREETATKTSDETLDAAGALGDTHTKESACDVVANGHVNKRLADAGDEAPSCPPHSEVRDGDSAGKAREPPPERLLTSDSAETASHMWLGSSVCIWEANEGEKCQDTSAEPQTRTCAIQATEEGMYTLSPLCATGPGGLLYVATEVPAGQREVEVHPRALLRSPLGSQAGGWGRTRSRRGGAERRHAPGRERHPAKEAVTTRAKIEEWRHAGDIEKFRKVCFLGRSNSASDSKTYVVEKPLAHPGPSLVASHEEARESGEPAKNERKGRTRHPGERKEGSNSMLDRHKLGSEETQETPGSVRSSGLKRPSLARLRGLPELLLRHATHFSRSPRVVGNDGSRDQTELSNEEITKSRERERCGSRSTRIQTLLSQSVLSPKEARNLRQRERKSSRERQGAERLCRRSEREGSASRPSSPASLRQPSRISAGSSSRPASSSPSAPSSVCPHKPASGLRQRGPRRAGSDVSAERREQNMPEEKRGDGGHCRLSLASGREPQRVSLPNMAFQRRPIMRAGVSPVPSKTPIISPASLPSSFQVFSTPRNLWCLSDSTPCRALRSTTTPESFAGSSVSSPVEVHSLASSSLSSPPCERLPDDPVDSMRLVKAKLRLARAAQRGPVSSLVSASPGKLNEKGRAFPCRSAASEGHGDDSQRFFQFLRKTFEAGGEGKYASVLRPLTSAARPSFVSGSSPFSPLFACPAFRQPPAPKAVTLTPEDKTARYSRCFSSSATLEDQRVAAIPLPHCSLTPASLLRNSRGAFSALEFQRLPTASCSGLAGEVLAAEWATRQKGEGDKRALFRDEKRARG

Target ORF information:

RefSeq Version	XM_018780014.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 hypothetical protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018780014.1

ORF Insert Sequence:

ATGGAGCGCT GGGGGTCTGC CCCTCCAACA CAGCATGGAG GGCAGAGAGC GCTGCCACGA 
CAGGATCGCT CGTCGCCTCC GTTGCGCAAA GACGTAAAGG GAGTTTCGCC GCCCATCCCG 
GTCGTTCCCT TTTCTTCTCA ATCTGGAAGC CGATCTCTTG GGTCTCCACA AGGAAATTAC 
ATGCCGTATC GGCACGAAGC AGCTTCTGAG TGTTCGGGGA AAAAAGGGTC TCATAGAATA 
CCGTCACGGA GTGTGTCCAG ATCGGGTTCT CCATCCACTG GAATGGACGC CAAGCCACTA 
TCGGTTCCAC GGCCTTTCCA TCGTTCCTGG TCGACGAATC CTCAACCAGG AAACGGCCAT 
TCTTCGCATG TTTCAGGAAG CAGTCGCCTC TCCCGGTCCT TAACAAGCCA CACACCGTTC 
CACAACGCTC CCAACACCGT CCTTCTTGTC GGAGGAAACT CTGCGAGCCA GCGAAGGCGA 
CTTACTGACG GAACTTATGT CCCGATTGTG GCGTTAACTG CTCCACCCTC AGAAGACCGA 
ACGGGAAAGC AGTGTTCGTC ATCACCTGCT CGGCAGCACT CGTCTGGGAG CCGTGCTAGC 
AGCGCTAACC ACCACTCCAC ACGTCACAGC TCTTCTCGGG TTATTCAGAA ATCGCTTTCT 
CGCAGCCGTA GTGTGTCCCA CCACCGCAGC CTGCAGAATT CTCATGTAGA TATCCAAAAG 
TCTCACAGTA ACGACCACCA CTCTCACGGC AGCGGCCGCC TTTCTCACAG TTCGCACCAC 
AGTCGGCACT CAAAGTACCG CCACGGGCAT CACCGGAGCT CTCGGAACCA CTCGCCTTCG 
CCTTCCCATC ACTCTCGACA CCGTGACAGT TCCAATCCCA GTCATTCTCG CGGAAAATTC 
GCGTCGCCAT CGAGGAACTA TGGTTCCAGA ACGTCGCATC GGCCGCATTC GCGGGACAGG 
AAGATTGTCG AGAATCCCCG AACTTCTCAT GACAGCCGTA CCGGATCCAC GCTTCCCGAT 
TTTTCTTTGT CGCCTTCTCG ATATGCCCCG CCTATCTCGC CGCCTCGAAC CCATCAAACG 
TCCTATGAGT TGGACAAAAC TGCGCCACCA TACCACCCCG TTGCCCACTA CAACTACAGC 
GAGCGGGATT TTTCTCCAAG ACATCAGTCG CCGCGTTCCG GCTTCCAGCC GTCTCACGCC 
ATTGAAAATA AGTATGGAAG AGGGACATCG CGTGTAGATG TTTTAGACAG ACTCCACTGC 
AATGCTCGAG TGAGTGAGAC CGACTCTTAC CGACCAGGAA TTTCGATAAT CGGAGGAAAG 
CAACTCAAAC CTATTCGTGC CCGTTCTCCG CGCCGTTCAC GCTGCTACGC AGCCAACGTC 
GACTCTGATG GACGGTTTTC GGCATTCTAC CAGTCCAATG CTCAACGTAC GATTTCTCCT 
AGCGATACCT GCCGTCCACG GGTAGTAGCA CGTCCACCAC CGTCGACTTC AAGATGCAGT 
CCGACCTGTG CACAATCGCG CCGTTTCGCT CCTCTGGATT ATTCAACTTC AAGCGCGACT 
CGTCCTCTGC GAAATGTCCC AGTGTACTTG CCTTCTCGGA GGAATTCGCC TTCCCGGCGT 
CCTCCTTCCC AAGAAGGCCG AACCCACTGC AGTCCAGTAG GCGGTCGATC GGGATGCAGA 
GCAGATCCCT CCTTGCGCGA CTGTTGCTCA GACATCCCTC CACGGGGCCA GTGGCTCTCA 
GGGAAGACAA GAACAAAACT CTCTGATTTT TGCCGTTCTG GTCGCCTTAC CCCGGTGACG 
GGATGCGCAA AAGCTGCACA GGAGGACTCG TTTTTTGGCA GCCTTCCCAA TTCTTCTTGC 
CAAGGAGAGA TGCACTCCTT CCGTCGACCG TTCCGAGCCA GCTCCTCGGA GCACTGTCCA 
CTTTCAGGGC TCCGTACACC GAGTCCGCTG TTAACTAGTG CGCACTGCCA TGGAGCTATT 
AATATGTCTT GTTCCTCTTT GCCGTCTGTC TGCGGGGGGT CTGCTCTTCC CGTGGATCTC 
TCACGTCGCT CGCCGCACTG TCACCCCGTG AGCACCATTC TGGGGGACTG CGTGACGCAA 
CGGAAGTCTG CGGCATGCCG GACAAATTCG CCCGCTGGCA GATGTTTCGA CAGCTTGGCG 
ACACAGTATC CACCTCCTCC TTGTCTCAAA CTTCACCGCC TTACCCACGA CGAGAGAAAC 
CATCTTCCGC GCGACAATCT GCCTCTGTCG CCTGGCCGAT GCATTCTTCA AGAACCCACG 
GATGAAGTGG AAGCGAGACG GGCTATCGAT ACGGCCGACC AGCTCCTGAA AAACGCGTCG 
CCCAGCGCAC GGGTTCGTGG GAATCTGAGT TATGAACAAG GCTTGTCGTC AGTTTCGTAC 
AAAAGGGAAG CGGATTCTTT GGGCAGGCGC TCCCCTTCAC TAGGAAGGCG GGACTTTGCC 
AGCTCGGCAG CCGCCTCTAC GCCTTTCCTG GAAAGCAAGC GTAGTGGGTG TTCTCGGGAG 
TTTTGTGAGT CGAATTTTCA GGTGTTCTCT AGCGCGCCGT ATCCCGAGCT CACAAGTGAA 
CGAAGTATCT GCTCTTCGCA GTCGCTCTCG AGCTCGAGAC GAAGACCACT GTGCTCTCGT 
CGACCCGACA CGGAAACGAC TCTTTTGCGC GCTTTGAAAT CCGGCCCATC TTCTCTCCCG 
GCCTCGGGTT CTTCATCGAC TTCTACCGCC CCGTTTCTGT CCTCTGTACC GGGAAGAATC 
GACAAAGGAA GCTGTCTCGG CCGTGTCCCG CTTGAGGGTA CCGGCCTGCG GTGCAGTCAC 
CACTGTGACT GTCTAGAGAC CCGCGACGAG TCGTGTCTTC CGAAGCGGAA AAGCATGGAG 
ACTTCCTCGT TCCTGTCTGT CTCGCACGAC AAGCCACGGC TTCCTGCTCG GGTCTCAGGC 
GAAATTGCCG CCGAGCGCCT GCGCCATAGA CTGGATCAAT GCCAGCGGGT GGAGGAGTGT 
AAAGACGGAC TTCCCGCCGG TCGCCGTGAC TCCGGCGGGT GCATCGACAG CGGCATACAT 
GCTGACACCT GCCACGGCGT TCATGCGGCC CGCCGGTCAC CAATTTCGCC GCTCTTCGAA 
GCGAGCCGAT GCACTCACCT TTCAGAGGTC GAAGCCATTG AGGCTGAAGT GGAATTCGTT 
GAGAAGAGGC GCCGCGCCCG CGAGAGGGGC CAAGAAGACA AGCGTACGGA GACCCTTCTG 
CGCTCCTTCA CCTGCCTCCC CCGTCTGTCC CTTCAAAACG CGAAGCGTAT CGACACTCAA 
CAGAAAGCTC TTCTAACCGC ACGATCGCGT TTTCCCGCCA AGTGTTGCTT TCCAGGGGAT 
TCTGAGGCCG ACGCCGCAGC ATGCAGCGAT CTGTCCGAAA GCCACACCCA GAACCTCGGT 
TCTGCCCTTG CGTGTGCCCA CCCGCCTAGC CCCCACACCC GCCAGCCCAG CATTCGCCTC 
GAGTCTCCGT CGGGTCTTTA CCTAGGCAGA TCGAGTGGCA GGCGCATAGC GGAAAGCAGC 
TGTCCGTCGT CTCTGGGGAC CCCGAAGAGC AATTTCGAGA CTTCAGTGTC TCTCTCTTCG 
GGCGCCTCGC GAGCGCAGCG TGTAGCTGAG ATCGTCGAGC GCCTGAGTCG AGTGCAAGAA 
GCGGAGTGCG CGAATCTGAG AAGACGCTCG GCGGAACGCG CCGCCCGGAG TCGTCTGACG 
CCTCCAGGTA TGAACATGCC GACCCTTGAA GGCTGCCGGC GGGCCAACGC GTGCACGGAA 
GAGATAAAGA GTTTGCAAGA AGCCTCTGCG CCTGAGGGCG CCTTCCCAGT CTGCCCCTTG 
CACGGTGGAG GCGCAAAAAA TCCGAGAATA AGAGACGAAG AGTGGAAGCG AGACCAGAAA 
CCTAGAGGGA CCGCACTGAA GCTGAACGCC GAGCAACGCT CGCAACTCGC AGCGGCCTGT 
GAGACCCGGA ACGCCCTCCT TGCGGCTCTA GCCTCACGAG CGCGACGCAG AAGAGAAGAA 
GCGACCGCGA GAAAGCGTCG CGATGGGGGA AGACACGCTG GAAGAACAGA TGGGTTCCAA 
AGACACCGGC ACGACGGTAG GGGCGAGAGG CACCTCTCGC CTTCTCTAGG GTCGCCTCTC 
AGCTCGCGAC CCGCTGCTTC TAGCGTTCCC AACTTGTCCA TTTCGTCAAA GTCGTGTCCT 
TCCTCTTCTT GTTCTGTCCA GACGGCCTCC TCAATCGCTT CCCGTCCGCC GTCGTCGATC 
CACTCTGGCA GTCGCGCCTC CTCCCTTTCC GCCGCTTCTC CCTCCCTACC GTCGCCCATC 
TCCCCTCACT CCGAGGAAAG ACACCCGAAG CGTTCAGGAG ACCTGAAGAG TTCAGAAGGT 
CAGAAGAGTT CAAGAGATCT GGTGTCTGTA CAGCTCTTGC AAGTCCCTTC CGCGAAAGGC 
TCCAAACCGC CGCGTCGCTC AGGCTCCAAC GCTTCAGCCC CAGACCAACC GGCCTCTCCT 
CGGCGCTCGG CTTCTCCAGT AGAATCTCCG AGAGACCTTG CCCCTCCCTC AAGAAGTCTC 
AGTCGTGCCT CTTCGGCGGC CAACTCGAGA AGCGACGGGA GTGTCTCTAC TAGCAGGCTT 
GCGGAGATAA AAAAAGCGAA CGAGGGCGAC AAGAAACAGG AGAGCAAGGG GGGGAAGAAG 
GAGGTGAAAG AAGAAAAAAA GAAAGAGAAG GCGGCGCGGA CGCTGTCGCC CTCGCCGACT 
GTGCAGAGCA TCAGCTCCCG CGCGTCTTCA ACGCGAAGCA AAGGGAATCC GGTGAAGCTT 
TCGTCTTCCG CTGACGCGCC ACACGCGCAG AGGAAAGAGA AAGGCAAAGT GGGGGGGAAG 
AGGCCGACGA AAGAAGTCTG GGACAAGACA AGAGACCAGA CCGCCCTCTC GCCGCTTCCT 
AGCGCGAGTG TGAGCAGAGC GTCGTCGACC GCGAGTGTCT CCCGAGGCCA CCACACGGCT 
CGCTCTAGAA ACAAAAATCT GGGCAAGACA GCGAAGAAAG AGAAACATAC AAAGTCTGTC 
AAGGCCCTGT CCCCGTCTCC CAGCAGGAGC CTGAGTCGCG CCGCTTCGCA TTCGAGCACG 
CGCAGCCACC ACAGGAGACT CTTGGGGAAG CCTGAGTCCA CCCAGGAGGG AGAGAAGAGA 
GCGAAGACGC ACAAGACCCA CACAAGCAGT TCTTCCTCAA CTTGCGTGAA GGGAAAGAAG 
GCGAGGGAGG CGCCTCCTGG AGAGCCGAAC AAAGAACTGA AGCGAGGCGC CTCGGCCAAC 
CCCTTGGTCG GCATGACTTC CCCTAAGCGC ATACACTCGA AGAAACCGCT GTCGCTGTCG 
CCCTCTCCGT CTCGGCAACA GCGCCATAAC GCGTCCCTGA AGAAGCAAAA ATCATCGTCT 
GCTCACGAGA GACACTCCTA TCGGCGCTCT GTGACGGCCT CAACTTTGCC GAGCCCTGAG 
GCTCTGGCAA GGGGAAGACG TATCTCCCGG GGTGGGTCTG CCCAGCGTGA GCAGGCACAA 
CAGAAAGAGA AACGCCGAGA CGAAGACGCC CGGAAACGCC CCCACGGATC CCAGCGACTG 
CCAAGCCAAC TGCACCACTC TCATGACAAG AAAAGCAAAA CACACAAAAG TGCCTCACCT 
TCAGTCTCCC CTTCAGCTGC TCATTCTCGC TCTAACTCGC ACTTGCCCTC TCGGTCTCCT 
TCTCATTCTC GTCTAGCTTC TGCTTCCTAT CTCTCTCCTT CGCGCGCCTC GTCCAGGCGA 
CACGGGCGAG AACACAGTTC GAGTTCGGTG GGTTCCAAGA CGCACAAGAC GAAAGATTCC 
GAGAGACACA AGAGGACGAA GGACGATCCG ACAAGCTCGC CACCAAGTCG CAAGGACCCG 
TCGCAAGCGG GTGGAAAAGA CGCGTCGCAT GGCCAGCATT GCCTGGCGGC GTACCCACTG 
GCTGCGCACT TGCCTCTGAC GCCTCGCGAG GTTCTGCCTT TTTCAGAGTG CTTCCTAGGG 
GAGGGGAAAC CGCGGGGCAA AGCTCCTGAA GCGTCTTCCC CCTCCACGCA GTTCTTCGCC 
TCTCTCGCGG ACCTGCTTTC GCCGACTTCT CGCGGAGCTC CTGATGGACG AGAACGGTCA 
CCAGCCTCTG CGGACGTCGT GCAAGAAACT CTGGCGAAAG CTGGACTTCT CTGCGGGTCT 
TTCGCTTCCC TCTTCTCCCC TGGATCTCTT GTCTCACAGG AAGCAGGACT CAAGCGGTGG 
AAGGAGGAAA CGAGGAACGA GAGCAACTCG GCACTGGCTG CGGGGCCGGA GAGTAATGGA 
AGAGGCGAGG CGACTCAGAC AGCCGACGAG GCTCCTGTAC ATCCGACAGC GCTCGTGGAT 
TCGCTGTTGA GGGAAATGAG CTCGAAAGTG GAAGGATCTT CTGTTTCGCG TTCTCTTCTG 
CTGACGTCGG AGGTTCGGCT ACGCGATCTC ATCGATGGCC AAACAACACA GGCATGCACC 
CCAGGGGGCT CGGTTGCGAC TTCCCAAGCT ACCTCCTTGT CTTCCTCTTT GCAGTTTGCA 
GACGCAAGTG CTACCCTCTC GTTCATTGCC GAGGAACTCG AGGCGACGTC TCAGCTATGC 
AGGAGACTCT CCGGAATGCC CCAGTCCCCT TCTACCTCAG CGTCCTCCAC TTCCTTCTTG 
TCTTCCTCCC TTTCATCCTC TTCTTCTTCC CTTTCGCCTC CTTTTTCGTT GCAGGACGTT 
CCTTCATCTG CAACGACGGC TCACAGCAGT GACTCTTCGC GAGGTGCAGG CTGCGGCCGC 
GGCTCATGTT CTGCTTCTCC CTCGTTGGTT TTCTCTTCTT GTCTCTCTGT TCCCTCTCTT 
GTCTCTGGGG GAGCCGACGG TGCTCAAGAT GCCGCGCTTT GCTCCGTGGC CGGGGCGGGT 
GAGGAGCTCT TCACGGGGCA GGAGACAGAA GAGACTTACA GAGAGAGAAA TGGGTGCAGT 
GAGCGGAACC AAGAAAATGA GGCCCGAGTA GACACAGATT CACCGACACC CGGAAACAGA 
GAGTTTTCAG ATAAAGCAGA CACCCCCAAA AATACACAAA ACCTTCGCTG CACCACGTGC 
CGTGCCTCTT CGTTTTCGTC TTATTCTTCC TCGTCGCTCT CTTCTTCGTC AACTCCCTGC 
TCTTCGTCTT CCTCGTCTCC CCGTTCTTCT TCTTCCTCGT CGCTCTCTTC TTCATGTTCT 
TCTTCGCTGG CGTTGTCTTC GTCCTCTTCC TCCACTTCTT CTTCCTCGTC GCTCTCTTCT 
TCATGTTCTT CTTCGCTGGC GTCGTCTTCG TCCTCTTCCT CCACTTCTTC TTCCTCGTCG 
CTCTCTTCTT CATGTTCTTC TTCGCTGGCG TCGTCTTCGT CCTCTTCCTC CACTTCTTCT 
TCCTCGTCGC TCTCTTCTTC ATGTTCTTCT TCGCTGGCGT CGTCTTCGTC CTCTTCCTCC 
ACTTCTTCTT CATCCTCGCC TTCTCGTTCT CGCGTGAGTC GCGAGACACA AGGAAACACA 
CGAGAGGATG AGCACAGCAT TTTGGATCTG TCCGAACTGC ATGCAGAACA GGACGCTGAA 
TCTGTCCGCG CTTTGAGCGA CTCGGCTTCC GAGTCGCTGT CTCCGTTTCT CTCGACTGCG 
GCTGAAATTC GTATGTTGCT TCCGGACAAA GAAGAGGTTG CCGCGCTCTT CTCCTTCGTT 
TCTTGGAAAA GCTGCTTTTC AGCTGATCCG AGCAAGCGCA GACGGTCGCG CAGAAACTCC 
AAAAGAGGCG CCGAGCCGTT GACGAGCGCA TGGCGTATAG GGGCACCCCA GAGCCTCTTG 
GGAGCAGCTA GCCACTTGGG GACAAGACCC TCTCTTGCCG AGGTTGCCGC AGGTTCCTTC 
TCTATGGTGC AGAACGGGGG CGACCAGGGA GACGAAGACG CGGGAGACAG ACAGACAGGG 
GACGGACACA GCGAGGAAGA AACAGAGACC GAAGACGAAG AGGTGGAGGA CAGCACAGGA 
CAGCGGAGGA AGCCGGCATC TACGAAGTTG AGAGATTTGC TGAGAGCCTT CAGAGACAAA 
CCTGGTGAAG CGGCGCACCC AGCGGCCGAA GAGACAGAGA CAGAGGAGGT CGACGAAGAA 
GAAAGGCAAG CGCTTCTACA GAGACAGGGG GCGAGCGAGA GAGGAGACAC GGCGGACGAC 
GAAGCCGCAC AAGCAGAGGC CCCCAGTTGC CTGGACGAGG CGGCCATAGA AGACCTTTGG 
CTGCTTCAAC AAACGAACGG GGAAACAAGG AAACTGCTGG ATAACGTATT CCATTGTTTG 
CAGCCCGTGA CTTCTGCCGC TTTCGACGGA GGGGAGGCGG CGTCTTCACC TCCGACTTTC 
TGGCGCCCCA GAGGCGCTGC TATGTCGCCT CGACGGTGCT TTCTCCCCTT GGTTTGCGAG 
CGAAGACAAG CAGAAGTCGA AGGTGGAGAG ACTGAAGAGG GCGCACTCGA GCGGCAAGAA 
TTGGCGCAAC GCGTTCAAAC CTTGCGCTCT GCCTTCACTG GGGCAGTCCC CTCTTTCTTC 
GCCGACTGTC GCTTTTTCTC TGCCAGTACA CCCCGCAGAT GTCCGACGGC AAGGCGCAGA 
GCCTTTTCCT CGGCTGGCTC GCCCCCCTGC CGCAGGGGAG ACGGAGACGG CGAGACAGGA 
GAAATCCAAC CGAGAAGGAG GACCTTGAGG CAGCTTCCCC AGAGAGAGGA GAAAGCAGCA 
ACCGGGCCAG CTTCGCCTCT GGCGCGCGCT TTGCCTTCTT CTGCACGTCG CCGAACAGAA 
GCGAGAAATC CCGAGGAAAC CCAAACGAAG GAGGAAGAAC ATGGGGAGGC AGGGAAGGTG 
GAAGAGCCTC TTTTCTGTGC AGAAGGTGGA GAAGAAGAGG AACGAGGGGG GGGAGCGAAG 
AAGCTGAAAG AGAGAGAGAC GAAGCAAGAG GAAGAGACGA GAGAGAGACC AGAAGAAGTG 
GAGGAAGCTA CGGTACTTGA ATCGAGTAGA GAGAGTCGCT CTGCCTTAGG CTGTCACTGC 
TTTGCATCGG CCTTTAGAAC GTCGGCCTCA TTGCCCCCTT TCGCTTCTGC CAGTAGCTCT 
GAGAACGCCT TGGGTAAGAG CGAATTGAAG GAGGACGCGG TAGCTTTGCA AATCGACGAC 
AGTCCCAAGG CGTCGGAATC CGGAGAAGGA AGGAGCGTTG AAGTCCCCGA ACCGGCAGAC 
GAGGGACGAA TGGCTTCAGG AACTCAAAAC GGTCCATGTC AGAATGAAGA AGAAGTGTAT 
GAGAAGAAAC CGACAGAACC AGGGACAGGA ATAAACGAGC AAGAGCATGC GCCAGCACCT 
CGTTCTTCCG CCCTCACGGC TGTCCATACT CCCGACGACA GACCCGTGGG CAAGGAAACA 
GGAGACAGAT ACGAACCCCT GGGGGAGAGG CAGACAGGCC ATGGAGAAGA CGCGAAAGGG 
GAGAGCGATC CAGATAAGAA ACAAGGCGCT CTGCAGCCTT CACTGTTGCA GGGGGAAACG 
CGAGTATCTG AAGAGCGAAG CAAACCCCCA CCATTGTTGT CAGAAGATTC ACAAGAAACA 
GAGGAAGAAC AGGAGTCAAT CATGGGGAGT TTCACCCTTC AAGAACTCTC TTCTTCGATC 
TCCGGCAAGC GTCCCTTTCG GCAGTCGGGT TTGAAGACAC CTGAGGCGGA CCCTCTCGCA 
TCGCCAGCTT GCTCTGAATT TACATTTGCC ATCGACTTTT CTCCGCAGCC GTCTGAACGT 
GCAGGCGCAG CTCACACCTC TGCGCCTGAG GCTTCTTTCT ACTCCAACTT ATCTTCGGAG 
ACACGACAGA GTCCAGAAAC TGAGTCTGTG TCAAAGGGGG TTCCTCAGAT GAAGTTAACC 
TTGCCCAGGT CTAGGGCGCC GTCTTCTTCT CTGTCGTCTT CTTCCTCTTC TGCCTCGTCT 
GTGTGCGTTT CTTCTTCCTC TTCTGCATCT TCCTGCTCCA CCTCGTCTAT GATTCTGTCT 
TTCTCGTCTT CTTCGTCTGT CCCGTCTCTC TCTTGGGGTC CTTCTTCCTC TACTCCTTCC 
TCTTCTTCTT TCGGGTGCGA CGAGAGTGAC CTACCGGCTC AGTTTGCCTC TTCTGCTTCG 
ACCGTCAAGG ACAGACTGCT ATGCTTAAGT GGGCATCAGA ACGTGGAGAG GGCTGCGAGC 
GAGACGGGAA GAGAAGAAAC GGCGACAAAG ACAAGCGACG AGACTCTGGA CGCCGCTGGA 
GCACTCGGAG ACACACACAC AAAAGAATCT GCATGTGACG TTGTCGCGAA TGGGCATGTC 
AACAAGAGGC TGGCCGACGC TGGCGACGAA GCTCCTTCGT GCCCACCCCA CAGCGAGGTC 
CGCGATGGAG ACAGTGCGGG CAAGGCGCGC GAGCCTCCAC CCGAGAGGCT TCTCACCAGC 
GACTCCGCCG AGACAGCGTC GCACATGTGG CTTGGCTCTT CAGTCTGCAT TTGGGAAGCA 
AACGAAGGAG AGAAATGCCA AGACACTTCA GCGGAGCCAC AGACTCGAAC TTGCGCCATC 
CAAGCAACAG AAGAAGGCAT GTACACGCTG TCGCCTCTTT GTGCGACGGG TCCTGGAGGT 
CTTCTGTACG TCGCCACAGA AGTACCAGCT GGACAACGGG AGGTGGAAGT ACACCCCAGA 
GCTCTTCTTC GCTCTCCTCT CGGCTCTCAG GCTGGCGGCT GGGGCCGTAC GAGGAGTCGC 
CGAGGCGGAG CCGAACGGAG GCACGCGCCC GGGCGGGAGA GACACCCAGC AAAAGAAGCT 
GTCACGACCC GGGCAAAAAT CGAGGAATGG AGGCATGCAG GGGACATCGA GAAGTTTAGA 
AAAGTCTGTT TCCTGGGACG TTCGAACTCT GCGTCTGACT CTAAAACGTA CGTTGTCGAA 
AAGCCTCTCG CCCATCCTGG TCCCTCTCTG GTAGCTTCCC ATGAGGAAGC GAGGGAGAGT 
GGGGAACCAG CAAAGAATGA GAGAAAAGGT AGAACTCGAC ATCCAGGTGA GAGAAAAGAA 
GGCTCAAATA GCATGCTGGA TCGACACAAA CTGGGTTCCG AAGAGACGCA GGAGACACCT 
GGATCTGTCC GTTCCTCCGG ACTAAAGCGG CCTTCTCTCG CGCGCCTCAG GGGGCTGCCA 
GAACTTCTGT TGAGACACGC GACGCATTTT TCTCGGAGCC CGAGGGTGGT TGGGAACGAC 
GGTTCGCGAG ACCAGACGGA GTTATCAAAC GAAGAAATAA CAAAGAGCCG TGAGAGGGAA 
CGTTGCGGTT CTCGTTCAAC GCGGATTCAG ACGCTTCTTT CTCAGTCCGT TTTGTCCCCC 
AAAGAGGCCA GAAATCTGCG ACAGCGAGAG AGGAAAAGTT CTCGCGAGCG ACAGGGTGCA 
GAACGCTTAT GCAGGAGGAG CGAACGCGAA GGTTCAGCAT CCAGGCCCTC GTCGCCTGCC 
TCTCTCCGTC AGCCTTCGCG TATCTCTGCA GGTTCATCTT CTCGTCCTGC TTCCTCTTCT 
CCCTCGGCGC CTTCGTCCGT CTGTCCCCAC AAACCTGCAT CAGGACTTCG CCAGAGAGGT 
CCTCGCCGTG CAGGCAGCGA TGTCTCAGCA GAGAGAAGGG AACAGAACAT GCCAGAGGAG 
AAGCGCGGCG ACGGAGGTCA CTGTCGTCTC TCCCTGGCGT CTGGGCGCGA GCCTCAAAGG 
GTCTCTCTTC CAAATATGGC CTTCCAAAGA AGACCCATCA TGCGCGCGGG CGTCTCACCG 
GTCCCCTCCA AAACTCCAAT CATCTCTCCC GCTTCGCTTC CTTCGTCGTT CCAAGTTTTC 
TCGACGCCGC GGAACTTGTG GTGTTTGTCA GACTCCACGC CTTGCCGCGC TTTACGTTCC 
ACGACAACGC CTGAGTCTTT TGCTGGCTCT TCCGTTTCTT CTCCCGTCGA GGTTCATTCA 
CTCGCTTCTT CGTCTTTGTC TTCCCCGCCT TGCGAGCGTC TTCCCGACGA CCCAGTCGAC 
AGCATGCGCC TCGTGAAGGC CAAACTTCGA CTCGCCCGAG CGGCGCAACG CGGCCCTGTC 
TCGTCCTTGG TCTCGGCTTC TCCGGGGAAA CTGAACGAGA AAGGCCGCGC GTTTCCTTGT 
CGCAGCGCTG CCTCAGAGGG ACATGGAGAC GACTCACAGA GGTTCTTTCA GTTCCTGCGC 
AAGACCTTTG AAGCAGGAGG AGAAGGTAAG TATGCGTCCG TTCTCCGGCC CCTCACGTCT 
GCAGCTCGTC CCTCGTTCGT GTCTGGCTCT TCACCGTTCT CTCCCTTGTT CGCGTGTCCT 
GCGTTTCGTC AGCCACCGGC TCCTAAAGCT GTGACATTGA CTCCAGAAGA CAAGACAGCC 
CGCTACAGCA GATGCTTTTC CTCGTCTGCG ACTCTCGAAG ACCAACGCGT CGCAGCCATC 
CCTTTACCCC ACTGTTCACT GACGCCTGCC AGTCTTCTCC GTAACTCCAG AGGTGCCTTT 
TCTGCCCTCG AGTTCCAGCG TTTGCCGACT GCCTCGTGTT CCGGCCTTGC CGGCGAGGTC 
CTTGCAGCGG AGTGGGCGAC GCGACAAAAA GGCGAAGGAG ACAAACGGGC TCTTTTCAGA 
GACGAGAAGC GCGCGCGTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_223725 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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