SPHKAP gene cDNA ORF clone, Taeniopygia guttata(zebra finch)

mRNA	Protein	Name
XM_030279837.1	XP_030135697.1	A-kinase anchor protein SPHKAP isoform X4
XM_030279834.1	XP_030135694.1	A-kinase anchor protein SPHKAP isoform X1
XM_030279835.1	XP_030135695.1	A-kinase anchor protein SPHKAP isoform X2
XM_030279839.1	XP_030135699.1	A-kinase anchor protein SPHKAP isoform X4
XM_030279841.1	XP_030135701.1	A-kinase anchor protein SPHKAP isoform X6
XM_012575464.1	XP_012430918.1	A-kinase anchor protein SPHKAP isoform X2
XM_030279836.1	XP_030135696.1	A-kinase anchor protein SPHKAP isoform X3
XM_012575463.1	XP_012430917.1	A-kinase anchor protein SPHKAP isoform X1
XM_030279843.1	XP_030135703.1	A-kinase anchor protein SPHKAP isoform X8
XM_030279844.1	XP_030135704.1	A-kinase anchor protein SPHKAP isoform X9
NM_001177531.1	NP_001171002.1	A-kinase anchor protein SPHKAP
XM_030279840.1	XP_030135700.1	A-kinase anchor protein SPHKAP isoform X5
XM_030279842.1	XP_030135702.1	A-kinase anchor protein SPHKAP isoform X7
XM_030279845.1	XP_030135705.1	A-kinase anchor protein SPHKAP isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTd00394	NM_001177531.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd16560	XM_012575463.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTd16561	XM_012575464.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51524	XM_030279834.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51525	XM_030279835.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51526	XM_030279836.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51527	XM_030279839.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51527	XM_030279837.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51528	XM_030279840.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51529	XM_030279841.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51530	XM_030279842.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51531	XM_030279843.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTd51532	XM_030279844.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTd51533	XM_030279845.1 Latest version!	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

SPHKAP ( NM_001177531.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> NP_001171002.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.

Price & Availability↑

CloneID	OTd00394
Clone ID Related Accession (Same CDS sequence)	NM_001177531.1
Accession Version	NM_001177531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from ABQF01030060.1 and ABQF01030061.1. On Apr 14, 2010 this sequence version replaced XM_002194848.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02689728, SAMN02689729 [ECO:0000350] ##Evidence-Data-END##

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
TTGGTTGACT TTAATGAGCA GAATTGCCTG AAGATTGGTG GCACCAGTTT GTTTGCCTAT 
ATTACAATGT CAATGTGCCT TCCCTATCAC CCTGGCAGCA CCAGTCCTCA GAGCAGATCG 
GATGCTGAAA TCATAGGAGA GACGAAAACA GCTGAAGAAT TTCCAGCCCA TCTCAGCAGC 
AGTGAAGAAA GCACTGGCAG CTGGTCCCAG CTGGCCAATG ATGAGGACAA TCCTGATGAC 
ACCAGCAGCT ACCTGCAGCT CAGTGAGCGC TCCCTGAGTG AATTAGTAGA AGAAAAGGTT 
TTCCTTAAAG AACAGACAGA AAACACTGAG GAAAGTACTT CTGAGCTTTC AGTGGGAACA 
GCCAACTGTC AAAAGGACCT CCTGGTGATA AACTTTGATC TGGAACCAGA GTGCCCTGAT 
GTGGAGCTGC GAGCCACCCT GCAGTGGATT GCTGCTTCTG AACTTGGAAT TCCAACTATC 
TATTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTGGT GCAGTTAGTT 
CAGCGGAAGT CCTGGAAAGT GGGGGACATC TTCCAAGCCG TGGTGCAGTA CTGCAAGCTC 
AGCGAGGAGG GCAGAGAGAT CACACCCAGC CTGTTTGACT GGCTGCTTGA ACTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171002.1
CDS	14..5053
Translation	MHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPFILEGNCINKDNAAAQVSETVNAGAENGISQPEDKSEQEILWQKELAEKSTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRGDPTTKHSSKTDGEDCAGVRKTDPPSQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNAYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCYRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKSLIVVQQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSLVDFNEQNCLKIGGTSLFAYITMSMCLPYHPGSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001177531.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177531.1

ORF Insert Sequence:

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
TTGGTTGACT TTAATGAGCA GAATTGCCTG AAGATTGGTG GCACCAGTTT GTTTGCCTAT 
ATTACAATGT CAATGTGCCT TCCCTATCAC CCTGGCAGCA CCAGTCCTCA GAGCAGATCG 
GATGCTGAAA TCATAGGAGA GACGAAAACA GCTGAAGAAT TTCCAGCCCA TCTCAGCAGC 
AGTGAAGAAA GCACTGGCAG CTGGTCCCAG CTGGCCAATG ATGAGGACAA TCCTGATGAC 
ACCAGCAGCT ACCTGCAGCT CAGTGAGCGC TCCCTGAGTG AATTAGTAGA AGAAAAGGTT 
TTCCTTAAAG AACAGACAGA AAACACTGAG GAAAGTACTT CTGAGCTTTC AGTGGGAACA 
GCCAACTGTC AAAAGGACCT CCTGGTGATA AACTTTGATC TGGAACCAGA GTGCCCTGAT 
GTGGAGCTGC GAGCCACCCT GCAGTGGATT GCTGCTTCTG AACTTGGAAT TCCAACTATC 
TATTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTGGT GCAGTTAGTT 
CAGCGGAAGT CCTGGAAAGT GGGGGACATC TTCCAAGCCG TGGTGCAGTA CTGCAAGCTC 
AGCGAGGAGG GCAGAGAGAT CACACCCAGC CTGTTTGACT GGCTGCTTGA ACTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_012575463.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_012430917.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OTd16560
Clone ID Related Accession (Same CDS sequence)	XM_012575463.1
Accession Version	XM_012575463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1432483200000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002199036.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Taeniopygia guttata Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
ACCAGTCCTC AGAGCAGATC GGATGCTGAA ATCATAGGAG AGACGAAAAC AGCTGAAGAA 
TTTCCAGCCC ATCTCAGCAG CAGTGAAGAA AGCACTGGCA GCTGGTCCCA GCTGGCCAAT 
GATGAGGACA ATCCTGATGA CACCAGCAGC TACCTGCAGC TCAGTGAGCG CTCCCTGAGT 
GAATTAGTAG AAGAAAAGGT TTTCCTTAAA GAACAGACAG AAAACACTGA GGAAAGTACT 
TCTGAGCTTT CAGTGGGAAC AGCCAACTGT CAAAAGGACC TCCTGGTGAT AAACTTTGAT 
CTGGAACCAG AGTGCCCTGA TGTGGAGCTG CGAGCCACCC TGCAGTGGAT TGCTGCTTCT 
GAACTTGGAA TTCCAACTAT CTATTTTAAG AAATCTCAGG AAAACAGAAT TGAAAAGTTT 
CTAGATGTGG TGCAGTTAGT TCAGCGGAAG TCCTGGAAAG TGGGGGACAT CTTCCAAGCC 
GTGGTGCAGT ACTGCAAGCT CAGCGAGGAG GGCAGAGAGA TCACACCCAG CCTGTTTGAC 
TGGCTGCTTG AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012430917.1
CDS	13..4953
Translation	MHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPFILEGNCINKDNAAAQVSETVNAGAENGISQPEDKSEQEILWQKELAEKSTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRGDPTTKHSSKTDGEDCAGVRKTDPPSQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNAYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCYRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKSLIVVQQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_012575463.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012575463.1

ORF Insert Sequence:

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
ACCAGTCCTC AGAGCAGATC GGATGCTGAA ATCATAGGAG AGACGAAAAC AGCTGAAGAA 
TTTCCAGCCC ATCTCAGCAG CAGTGAAGAA AGCACTGGCA GCTGGTCCCA GCTGGCCAAT 
GATGAGGACA ATCCTGATGA CACCAGCAGC TACCTGCAGC TCAGTGAGCG CTCCCTGAGT 
GAATTAGTAG AAGAAAAGGT TTTCCTTAAA GAACAGACAG AAAACACTGA GGAAAGTACT 
TCTGAGCTTT CAGTGGGAAC AGCCAACTGT CAAAAGGACC TCCTGGTGAT AAACTTTGAT 
CTGGAACCAG AGTGCCCTGA TGTGGAGCTG CGAGCCACCC TGCAGTGGAT TGCTGCTTCT 
GAACTTGGAA TTCCAACTAT CTATTTTAAG AAATCTCAGG AAAACAGAAT TGAAAAGTTT 
CTAGATGTGG TGCAGTTAGT TCAGCGGAAG TCCTGGAAAG TGGGGGACAT CTTCCAAGCC 
GTGGTGCAGT ACTGCAAGCT CAGCGAGGAG GGCAGAGAGA TCACACCCAG CCTGTTTGAC 
TGGCTGCTTG AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_012575464.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_012430918.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTd16561
Clone ID Related Accession (Same CDS sequence)	XM_012575464.1
Accession Version	XM_012575464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1432483200000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002199036.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Taeniopygia guttata Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
ACCAGTCCTC AGAGCAGATC GGATGCTGAA ATCATAGGAG AGACGAAAAC AGCTGAAGAA 
TTTCCAGCCC ATCTCAGCAG CAGTGAAGAA AGCACTGGCA GCTGGTCCCA GCTGGCCAAT 
GATGAGGACA ATCCTGATGA CACCAGCAGC TACCTGCAGC TCAGTGAGCG CTCCCTGAGC 
AATGGCAACA GCAGTACAAC TAGCAGTCTT GGCATTATGG ACCTGGAAAT TTATCAGGAG 
AACGTGCCAT CTTCTCCTAT GATTAATGAA TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA 
CAGACAGAAA ACACTGAGGA AAGTACTTCT GAGCTTTCAG TGGGAACAGC CAACTGTCAA 
AAGGACCTCC TGGTGATAAA CTTTGATCTG GAACCAGAGT GCCCTGATGT GGAGCTGCGA 
GCCACCCTGC AGTGGATTGC TGCTTCTGAA CTTGGAATTC CAACTATCTA TTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTGGTGC AGTTAGTTCA GCGGAAGTCC 
TGGAAAGTGG GGGACATCTT CCAAGCCGTG GTGCAGTACT GCAAGCTCAG CGAGGAGGGC 
AGAGAGATCA CACCCAGCCT GTTTGACTGG CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012430918.1
CDS	13..5040
Translation	MHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPFILEGNCINKDNAAAQVSETVNAGAENGISQPEDKSEQEILWQKELAEKSTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRGDPTTKHSSKTDGEDCAGVRKTDPPSQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNAYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCYRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKSLIVVQQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPSSPMINELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_012575464.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012575464.1

ORF Insert Sequence:

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCATCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCATTCATT CTTGAAGGGA ACTGCATTAA CAAAGATAAT 
GCAGCTGCTC AGGTCTCTGA AACTGTGAAT GCTGGGGCTG AAAATGGCAT CTCACAGCCT 
GAAGATAAGT CAGAGCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AAGTACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGGTGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTCC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACGCATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCGAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTAT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAACGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCATT GAGCGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATTAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAGTTT AATTGTTGTC CAGCAGGATT CCTTTGATTA TACAAGCCGA 
GAGCACGTGC TGGCAAGCAA ACATCCCCAA AGCACAACCC AGGTACAGCC CAAACCCACA 
ATGGAGGAAG TGAATTTGGA TGAGAAAAAA GAGCACATGA AGAGCCCTGG AAGCCTCCCT 
GCAGGACAGC TTAGGGAAGT GCCTTTAATT CAGATAGAAA CTGATCAGCG AGATGAGCCA 
GATAAAGACT CAGAGTCCTT GACTTCGTGT GGCCCATCTG GAAAGGGGCA GCAAAGCAAA 
GCAAAGCCTC CAGAAGCTTT GGATGGGAAA CACGTGGTTT CTAGTTCCCC AGTGAGTAGC 
ACCAGTCCTC AGAGCAGATC GGATGCTGAA ATCATAGGAG AGACGAAAAC AGCTGAAGAA 
TTTCCAGCCC ATCTCAGCAG CAGTGAAGAA AGCACTGGCA GCTGGTCCCA GCTGGCCAAT 
GATGAGGACA ATCCTGATGA CACCAGCAGC TACCTGCAGC TCAGTGAGCG CTCCCTGAGC 
AATGGCAACA GCAGTACAAC TAGCAGTCTT GGCATTATGG ACCTGGAAAT TTATCAGGAG 
AACGTGCCAT CTTCTCCTAT GATTAATGAA TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA 
CAGACAGAAA ACACTGAGGA AAGTACTTCT GAGCTTTCAG TGGGAACAGC CAACTGTCAA 
AAGGACCTCC TGGTGATAAA CTTTGATCTG GAACCAGAGT GCCCTGATGT GGAGCTGCGA 
GCCACCCTGC AGTGGATTGC TGCTTCTGAA CTTGGAATTC CAACTATCTA TTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTGGTGC AGTTAGTTCA GCGGAAGTCC 
TGGAAAGTGG GGGACATCTT CCAAGCCGTG GTGCAGTACT GCAAGCTCAG CGAGGAGGGC 
AGAGAGATCA CACCCAGCCT GTTTGACTGG CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279834.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135694.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OTd51524
Clone ID Related Accession (Same CDS sequence)	XM_030279834.1
Accession Version	XM_030279834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135694.1
CDS	222..5390
Translation	MSRPSTGCRAAARSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279834.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279834.1

ORF Insert Sequence:

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279835.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135695.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OTd51525
Clone ID Related Accession (Same CDS sequence)	XM_030279835.1
Accession Version	XM_030279835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CATGCCATCT 
TCTCCTATGA TTAATGAATT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGAAAAC 
ACTGAGGGTA AGTTGAACTA TATCAATTCA AACATACAGT TTGAAGATAA TACAAGCAAT 
TGCACAGTAA CTAGCGGGAT TATTTTTCCA GAAAGTACTT CTGAGCTTTC AGTGGGAACA 
GCCAACTGTC AAAAGGACCT CCTGGTGATA AACTTTGATC TGGAACCAGA GTGCCCTGAT 
GTGGAGCTGC GAGCCACCCT GCAGTGGATT GCTGCTTCTG AACTTGGAAT TCCAACTATC 
TATTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTGGT GCAGTTAGTT 
CAGCGGAAGT CCTGGAAAGT GGGGGACATC TTCCAAGCCG TGGTGCAGTA CTGCAAGCTC 
AGCGAGGAGG GCAGAGAGAT CACACCCAGC CTGTTTGACT GGCTGCTTGA ACTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135695.1
CDS	94..5253
Translation	MAGRPPPAAPSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279835.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279835.1

ORF Insert Sequence:

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CATGCCATCT 
TCTCCTATGA TTAATGAATT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGAAAAC 
ACTGAGGGTA AGTTGAACTA TATCAATTCA AACATACAGT TTGAAGATAA TACAAGCAAT 
TGCACAGTAA CTAGCGGGAT TATTTTTCCA GAAAGTACTT CTGAGCTTTC AGTGGGAACA 
GCCAACTGTC AAAAGGACCT CCTGGTGATA AACTTTGATC TGGAACCAGA GTGCCCTGAT 
GTGGAGCTGC GAGCCACCCT GCAGTGGATT GCTGCTTCTG AACTTGGAAT TCCAACTATC 
TATTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTGGT GCAGTTAGTT 
CAGCGGAAGT CCTGGAAAGT GGGGGACATC TTCCAAGCCG TGGTGCAGTA CTGCAAGCTC 
AGCGAGGAGG GCAGAGAGAT CACACCCAGC CTGTTTGACT GGCTGCTTGA ACTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279836.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135696.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

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CloneID	OTd51526
Clone ID Related Accession (Same CDS sequence)	XM_030279836.1
Accession Version	XM_030279836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACT TTGAATCTCC ACTGATGCAT GAAGTCCCAG AGCGCCAAGG CAGCAGCACA 
GACAGTTCTG CAAGCAGTTT TGGAAGCTCC GTCACAGCAT GTAAGAAGAT TCTCTGCAGT 
AACAGTCTGC TAGAATCAAC AGACTACTGG CTGCAGAATC AGAGGACACC ATGCCAAATT 
GGCTTTCTAG AAGACAAGTC AGAAAGCAAC TGTGCCTCAG TTTGTTTTGT GAATTTAGAT 
GCCAACAGAG ATGATTGCAG TGATGAGCAA GTGAAACAGA AATTGATCAG CGTCTCACCC 
AGTCTCCCCA AACTGATCAG TTCGATGAAC GTACAGCCTC CGAAGGAAAA TGAAATAGTA 
CTGCTGAGTG GATTGACATC AGGAAACCTT CAGGCCGGTT ATGAGGTTCC CCAGTGTCCT 
TGGCTGGCAG ATGTCTGCTT GGTTCAGTGT GCGAGGGGGG ACAGGAGGAA CAGCGCAAGC 
TGCATTATTT TTGAAATAAA CAAGTTTCTG ATTGGACTTG AGCTCGTTCA GGAGAGGCAG 
CTGCAGATAG AAACTCGTGT CCTAAAGCCT GAGGATGACA CAAACTGCTC CGTGTCCTCA 
ATAGAAGAAG ATTTCCTCAC GGCCTCTGAG CACCTCGAGG ATGACAACGA GGCTGATGAA 
TATAAAGCTG GTCATGAAAA ATTAAATGCT TCAGAAGCGT CTTCAGATGT CAAAAAAAAT 
AAAGGAAAGG GATTTGAAAA CATTCACTAT AGAAAAGCCA GGTTGCCGTC CATTCTTGAA 
GGGAGCTGCA TTAATAAAGA TAATGCAGCT GCTCAGGTTT CTGAAACTGT GAATGCTGGG 
GCTGAACATG GCATCTCACA GCCTGAAGAT AAGTCAGACC AGGAAATACT GTGGCAGAAG 
GAATTAGCTG AAAAAGATAC TTCATCATTT AGTACTACTA ATTTGACTAG TGGAGCTGAA 
AACTCGGCAC TCATGTTAGA AGATGTATCT GTAACCAAAA TGGCTAAAGA GGAGCTGGAG 
CCTCGGGATG ACCCCACAAC GAAACACAGC AGCAAGACAG ATGGGGAAGA TTGTGCAGGT 
GTCAGGAAAA CTGATCCCCC CTTCCAGAAT GAGCAGGCGA CCACAGGTCA GTACGCCACA 
AATTTAGCAG AATCTGTTCT GCAGGATGCA TTCATTAGAC TGTCACAGTC TCGACCCAGT 
TTCAGTGAGG AGGCTGCAGT CAGCATCTCT GCAGGAAGCT CCTGTAAGTC AGAAGATGTA 
TCTGCTTCCC GATCATGGAA CGAACTCCCA AAGATCATCA TAGTGCAGAG TCCGGACAGT 
TCTGAGCACG TACCGGACTG GCCAGGCTCT GCCTTCCCCA GCCCCAGCCC CTGGGCTGAG 
GCAGAAAGCT CTGCTGAAGT CTCAGATTAC TTTGAGGAAG AACATTCAAA CGGACATGAC 
CAAAGTGCAC TGGAAGTGGC TCTGGCTTGT GCAGCCACTG TCATTGGAAC CATTTCCAGT 
CCCCAGGCTG TGGAAAAATT CCGCTGGGAG CAGGAAGTGG CAGACTCGAG TGGAGTGGTT 
GCTAATGAAG AGGTGCAAGC AGCACCCTCA CAGCTCCTTG ACGACTGTGC TGGCACAGAA 
TACTCGTTCC CATCTGCGCT CTGTGGCATG ACTCAAGTGG CAAGTGCTGT AGCCATCTGT 
GGCCTGGGGG AAACAAAGGA GGAGAAATAC CCTGCAGCTT CCAGTGGACT TCTGTCTGCT 
GCTCAGGCCT CTGCAGCCAT TACTCTGCAT TGCAGCTTAG CTGTAGGAAG CAGCATGGAG 
AAGCTGAACG AGAGCATTGC AGAGGCGCTT CTGAAAGAGG CATCCCTCAT TCTGACAAAA 
CCTAACACAT ACAAAAATGT AGGGCATTTT ATGGAATCCA TAAATGGGAA AATTATTGAA 
ACAGCAGCAA GACCACATAT TCCACGTGCT GATGGAGTAA TCAGGGATGA ACTTGCACAA 
AACTTATCCA ATATTATTCT ACGACATTCT ATAGAAGAAG TTAGGAAGAA GAGACAGCTA 
CAAGCCCATT CGGAGGATGG CTTAAGTACA CAAGACTTTT TCACAGAGAC TGCAAACGAG 
TTGCTTTTTA ATATAATATA TTTCACTTGC AAGAAGATTA ACGACATAAG GCAACTCGAA 
GAGTGTTCTC CTCTCTTTTC TGAAAGCGCA AAAGCAGAGA AAGTAACAAG AGCAGAAGGA 
TGGCCAACCC TGGCAACAGC GTGTGAAACT TCACACAGCC CCCTTGATCA CTCTGTTGCT 
AAGCCATTTG GTACATCCTA CAGCACCAGT ACTAGTAAAG ATCTTGAAAA GGTCACAAGC 
ACTTGCAAGA GTGATATCAA AGATGTAAAT AGCAATGAAG GTTCCACACT GAATTCAGAA 
CCAAGTGTAG ATACAGGGCA TAACACACTT GGTGCAAAAA CATCACCCAA GAAGAGATAC 
CTGAAAAGAG CTGCACGAGA TTGTTACAAA TCCCCAAATC AGAGTAACAA TCATCATCAG 
AAGAAAGACT ACAGATCATT TTCAGACAGA GAAAATACCT TTGCAAACAG TGAATGCAGG 
CACGGTGTTC AAGAACAGCT GTCTTCCAGT GCCACCATAA ATACAGAAAA CCAAGCCAAG 
ATTAAGTGTG ATTCTGTGCT AAATAATGAT GTCCAACTTA GCTTGTCTTT GTTAGGAAAC 
CATGTGTTGC TTCCTTCTCA GCCTGTGCTA CAGGTGAAAA ATTCAAGGGA CAAATATTGT 
ATAACAGACT TTGCGGAAGA ATTGGCAGAA ACAGTTGTCT CTATGGCAAC AGAAATAGCT 
GCCATTTGTC TAGAAAATTC AAATGGCAAG CAACCCTGGT TCTGTGCATG GAAGAGAGGC 
AGTGAATATC TGATGACCCA GAGTTTATCA TGCAGAACCA TGAAAAGGAA GAAGGAAACC 
CATACCAATG GTTCTGTGGT TCGGAAGCAC AGGGCACCTC GGCTTAGTGA GATCAAAAGA 
AAAACAGATG AGCATCCTGA GCTAAAGGAA AAATTGATGA ATCGAGTAGT AGATGAATCT 
ATAAACCTTG AGGACACACC AGATTCAGTC AATCTCTTTG CAAATGAAGT GGCTGCCAAG 
ATCATGAACC TCACTGAACT CTCCATGGTT GATAGCATCT GGCAAGGTCC AAACCACCCC 
AGGAACAGAC TGCACTGTGA AAGGTGGAGC CGAGCCAAGG CCTCAAGCTG TGAGAGCATA 
CCAGAGGAGG ACTCAGATTC CAAAGCCTCT TTCAATACCC TAGGCCTCAT GAACAGCTTT 
GGTCACTCTC TCAGCCAGAC AAGTTCTGTC TCAAAGCAGT CTAGTTGTGA GAGCATTACA 
GATGAATTTT CAAGATTTAT GGTGAACCAG ATGGAAAACG AAGGAAGAGG ATTTGATTTA 
TTACTGGATT ATTATGCAGG AAAAAATGCA AACAGTATCT TAACTTCTGC CTTGCAACAG 
GTAGCCAAGA AAAATAGCCA TCTTAATGTA AGACCAAGTT GCCCATCCAA ACAGTCCAGC 
ACAGAAAGTA TAACAGAAGA GTTTTATAGG TATATGCTAA GAGAAATCGA AAAGGAAAAT 
AAAGATAATG CATCATCCTC TGGGAATTCA AAGGACTGGT GTGGCAGTTT GCTGGCACCC 
TCTCTACGGT CACCTTTCTG CTTTAGGCAG TCGTCGATGC CTGACAGTAG ATCCTCAGGC 
TCCAGGCTTA CAGTGAATGT CCCAATTAAA GCAAATTCCT TAGATGGGTT TGCCCACCAT 
CGCCAAGATT CACTAAGTGT ACAGCCCGTC AGTACCATGG CTTCTGCAGG GCTCTGCAAG 
TCTGACTCCT GCCTGTACCA GAGAGGCAAG ACTGACCAGA TCACAGACAT GCTGATCCAC 
GAGACCTGGG CCAGTTCCAT TGAGTCTCTG ATGCGCAAGA ACAAAATCAT AGCAGATGAG 
GCAGAGGCTA CAGAGGCAGA CCAGTTTCAC AGTGATTCTC CACCACACGT GGAACAATAT 
GCAAAAAGAC TGGCTGCAAA TATTGTTGAA AGTGGTAAAA ATTTAATTGT TGTCCAGGAT 
TCCTTTGATT ATACAAGCCG AGAGCACGTG CTGGCAAGCA AACATCCCCA AAGCACAACC 
CAGGTACAGC CCAAACCCAC AATGGAGGAA GTGAATTTGG ATGAGAAAAA AGAGCACATG 
AAGAGCCCTG GAAGCCTCCC TGCAGGACAG CTTAGGGAAG TGCCTTTAAT TCAGATAGAA 
ACTGATCAGC GAGATGAGCC AGATAAAGAC TCAGAGTCCT TGACTTCATG TGGCCCATCT 
GGAAAGGGGC AGCAAAGCAA AGCAAAGCCT CCAGAAGCTT TGGATGGGAA ACACGTGGTT 
TCCAGTTCCC CAGTAAGTAG CACCAGTCCT CAGAGCAGAT CGGATGCTGA AATCATAGGA 
GAGACGAAAA CAGCTGAAGA ATTTCCAGCC CATCTCAGCA GCAGTGAAGA AAGCACTGGC 
AGCTGGTCCC AGCTGGCCAA TGATGAGGAC AATCCTGATG ACACCAGCAG CTACCTGCAG 
CTCAGTGAGC GCTCCCTGAG CAATGGCAAC AGCAGTACAA CTAGCAGTCT TGGCATTATG 
GACCTGGAAA TTTATCAGGA GAACATGCCA TCTTCTCCTA TGATTAATGA ATTAGTAGAA 
GAAAAGGTTT TCCTTAAAGA ACAGACAGAA AACACTGAGG GTAAGTTGAA CTATATCAAT 
TCAAACATAC AGTTTGAAGA TAATACAAGC AATTGCACAG TAACTAGCGG GATTATTTTT 
CCAGAAAGTA CTTCTGAGCT TTCAGTGGGA ACAGCCAACT GTCAAAAGGA CCTCCTGGTG 
ATAAACTTTG ATCTGGAACC AGAGTGCCCT GATGTGGAGC TGCGAGCCAC CCTGCAGTGG 
ATTGCTGCTT CTGAACTTGG AATTCCAACT ATCTATTTTA AGAAATCTCA GGAAAACAGA 
ATTGAAAAGT TTCTAGATGT GGTGCAGTTA GTTCAGCGGA AGTCCTGGAA AGTGGGGGAC 
ATCTTCCAAG CCGTGGTGCA GTACTGCAAG CTCAGCGAGG AGGGCAGAGA GATCACACCC 
AGCCTGTTTG ACTGGCTGCT TGAACTGGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135696.1
CDS	20..5152
Translation	MSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279836.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279836.1

ORF Insert Sequence:

ATGAGTAACT TTGAATCTCC ACTGATGCAT GAAGTCCCAG AGCGCCAAGG CAGCAGCACA 
GACAGTTCTG CAAGCAGTTT TGGAAGCTCC GTCACAGCAT GTAAGAAGAT TCTCTGCAGT 
AACAGTCTGC TAGAATCAAC AGACTACTGG CTGCAGAATC AGAGGACACC ATGCCAAATT 
GGCTTTCTAG AAGACAAGTC AGAAAGCAAC TGTGCCTCAG TTTGTTTTGT GAATTTAGAT 
GCCAACAGAG ATGATTGCAG TGATGAGCAA GTGAAACAGA AATTGATCAG CGTCTCACCC 
AGTCTCCCCA AACTGATCAG TTCGATGAAC GTACAGCCTC CGAAGGAAAA TGAAATAGTA 
CTGCTGAGTG GATTGACATC AGGAAACCTT CAGGCCGGTT ATGAGGTTCC CCAGTGTCCT 
TGGCTGGCAG ATGTCTGCTT GGTTCAGTGT GCGAGGGGGG ACAGGAGGAA CAGCGCAAGC 
TGCATTATTT TTGAAATAAA CAAGTTTCTG ATTGGACTTG AGCTCGTTCA GGAGAGGCAG 
CTGCAGATAG AAACTCGTGT CCTAAAGCCT GAGGATGACA CAAACTGCTC CGTGTCCTCA 
ATAGAAGAAG ATTTCCTCAC GGCCTCTGAG CACCTCGAGG ATGACAACGA GGCTGATGAA 
TATAAAGCTG GTCATGAAAA ATTAAATGCT TCAGAAGCGT CTTCAGATGT CAAAAAAAAT 
AAAGGAAAGG GATTTGAAAA CATTCACTAT AGAAAAGCCA GGTTGCCGTC CATTCTTGAA 
GGGAGCTGCA TTAATAAAGA TAATGCAGCT GCTCAGGTTT CTGAAACTGT GAATGCTGGG 
GCTGAACATG GCATCTCACA GCCTGAAGAT AAGTCAGACC AGGAAATACT GTGGCAGAAG 
GAATTAGCTG AAAAAGATAC TTCATCATTT AGTACTACTA ATTTGACTAG TGGAGCTGAA 
AACTCGGCAC TCATGTTAGA AGATGTATCT GTAACCAAAA TGGCTAAAGA GGAGCTGGAG 
CCTCGGGATG ACCCCACAAC GAAACACAGC AGCAAGACAG ATGGGGAAGA TTGTGCAGGT 
GTCAGGAAAA CTGATCCCCC CTTCCAGAAT GAGCAGGCGA CCACAGGTCA GTACGCCACA 
AATTTAGCAG AATCTGTTCT GCAGGATGCA TTCATTAGAC TGTCACAGTC TCGACCCAGT 
TTCAGTGAGG AGGCTGCAGT CAGCATCTCT GCAGGAAGCT CCTGTAAGTC AGAAGATGTA 
TCTGCTTCCC GATCATGGAA CGAACTCCCA AAGATCATCA TAGTGCAGAG TCCGGACAGT 
TCTGAGCACG TACCGGACTG GCCAGGCTCT GCCTTCCCCA GCCCCAGCCC CTGGGCTGAG 
GCAGAAAGCT CTGCTGAAGT CTCAGATTAC TTTGAGGAAG AACATTCAAA CGGACATGAC 
CAAAGTGCAC TGGAAGTGGC TCTGGCTTGT GCAGCCACTG TCATTGGAAC CATTTCCAGT 
CCCCAGGCTG TGGAAAAATT CCGCTGGGAG CAGGAAGTGG CAGACTCGAG TGGAGTGGTT 
GCTAATGAAG AGGTGCAAGC AGCACCCTCA CAGCTCCTTG ACGACTGTGC TGGCACAGAA 
TACTCGTTCC CATCTGCGCT CTGTGGCATG ACTCAAGTGG CAAGTGCTGT AGCCATCTGT 
GGCCTGGGGG AAACAAAGGA GGAGAAATAC CCTGCAGCTT CCAGTGGACT TCTGTCTGCT 
GCTCAGGCCT CTGCAGCCAT TACTCTGCAT TGCAGCTTAG CTGTAGGAAG CAGCATGGAG 
AAGCTGAACG AGAGCATTGC AGAGGCGCTT CTGAAAGAGG CATCCCTCAT TCTGACAAAA 
CCTAACACAT ACAAAAATGT AGGGCATTTT ATGGAATCCA TAAATGGGAA AATTATTGAA 
ACAGCAGCAA GACCACATAT TCCACGTGCT GATGGAGTAA TCAGGGATGA ACTTGCACAA 
AACTTATCCA ATATTATTCT ACGACATTCT ATAGAAGAAG TTAGGAAGAA GAGACAGCTA 
CAAGCCCATT CGGAGGATGG CTTAAGTACA CAAGACTTTT TCACAGAGAC TGCAAACGAG 
TTGCTTTTTA ATATAATATA TTTCACTTGC AAGAAGATTA ACGACATAAG GCAACTCGAA 
GAGTGTTCTC CTCTCTTTTC TGAAAGCGCA AAAGCAGAGA AAGTAACAAG AGCAGAAGGA 
TGGCCAACCC TGGCAACAGC GTGTGAAACT TCACACAGCC CCCTTGATCA CTCTGTTGCT 
AAGCCATTTG GTACATCCTA CAGCACCAGT ACTAGTAAAG ATCTTGAAAA GGTCACAAGC 
ACTTGCAAGA GTGATATCAA AGATGTAAAT AGCAATGAAG GTTCCACACT GAATTCAGAA 
CCAAGTGTAG ATACAGGGCA TAACACACTT GGTGCAAAAA CATCACCCAA GAAGAGATAC 
CTGAAAAGAG CTGCACGAGA TTGTTACAAA TCCCCAAATC AGAGTAACAA TCATCATCAG 
AAGAAAGACT ACAGATCATT TTCAGACAGA GAAAATACCT TTGCAAACAG TGAATGCAGG 
CACGGTGTTC AAGAACAGCT GTCTTCCAGT GCCACCATAA ATACAGAAAA CCAAGCCAAG 
ATTAAGTGTG ATTCTGTGCT AAATAATGAT GTCCAACTTA GCTTGTCTTT GTTAGGAAAC 
CATGTGTTGC TTCCTTCTCA GCCTGTGCTA CAGGTGAAAA ATTCAAGGGA CAAATATTGT 
ATAACAGACT TTGCGGAAGA ATTGGCAGAA ACAGTTGTCT CTATGGCAAC AGAAATAGCT 
GCCATTTGTC TAGAAAATTC AAATGGCAAG CAACCCTGGT TCTGTGCATG GAAGAGAGGC 
AGTGAATATC TGATGACCCA GAGTTTATCA TGCAGAACCA TGAAAAGGAA GAAGGAAACC 
CATACCAATG GTTCTGTGGT TCGGAAGCAC AGGGCACCTC GGCTTAGTGA GATCAAAAGA 
AAAACAGATG AGCATCCTGA GCTAAAGGAA AAATTGATGA ATCGAGTAGT AGATGAATCT 
ATAAACCTTG AGGACACACC AGATTCAGTC AATCTCTTTG CAAATGAAGT GGCTGCCAAG 
ATCATGAACC TCACTGAACT CTCCATGGTT GATAGCATCT GGCAAGGTCC AAACCACCCC 
AGGAACAGAC TGCACTGTGA AAGGTGGAGC CGAGCCAAGG CCTCAAGCTG TGAGAGCATA 
CCAGAGGAGG ACTCAGATTC CAAAGCCTCT TTCAATACCC TAGGCCTCAT GAACAGCTTT 
GGTCACTCTC TCAGCCAGAC AAGTTCTGTC TCAAAGCAGT CTAGTTGTGA GAGCATTACA 
GATGAATTTT CAAGATTTAT GGTGAACCAG ATGGAAAACG AAGGAAGAGG ATTTGATTTA 
TTACTGGATT ATTATGCAGG AAAAAATGCA AACAGTATCT TAACTTCTGC CTTGCAACAG 
GTAGCCAAGA AAAATAGCCA TCTTAATGTA AGACCAAGTT GCCCATCCAA ACAGTCCAGC 
ACAGAAAGTA TAACAGAAGA GTTTTATAGG TATATGCTAA GAGAAATCGA AAAGGAAAAT 
AAAGATAATG CATCATCCTC TGGGAATTCA AAGGACTGGT GTGGCAGTTT GCTGGCACCC 
TCTCTACGGT CACCTTTCTG CTTTAGGCAG TCGTCGATGC CTGACAGTAG ATCCTCAGGC 
TCCAGGCTTA CAGTGAATGT CCCAATTAAA GCAAATTCCT TAGATGGGTT TGCCCACCAT 
CGCCAAGATT CACTAAGTGT ACAGCCCGTC AGTACCATGG CTTCTGCAGG GCTCTGCAAG 
TCTGACTCCT GCCTGTACCA GAGAGGCAAG ACTGACCAGA TCACAGACAT GCTGATCCAC 
GAGACCTGGG CCAGTTCCAT TGAGTCTCTG ATGCGCAAGA ACAAAATCAT AGCAGATGAG 
GCAGAGGCTA CAGAGGCAGA CCAGTTTCAC AGTGATTCTC CACCACACGT GGAACAATAT 
GCAAAAAGAC TGGCTGCAAA TATTGTTGAA AGTGGTAAAA ATTTAATTGT TGTCCAGGAT 
TCCTTTGATT ATACAAGCCG AGAGCACGTG CTGGCAAGCA AACATCCCCA AAGCACAACC 
CAGGTACAGC CCAAACCCAC AATGGAGGAA GTGAATTTGG ATGAGAAAAA AGAGCACATG 
AAGAGCCCTG GAAGCCTCCC TGCAGGACAG CTTAGGGAAG TGCCTTTAAT TCAGATAGAA 
ACTGATCAGC GAGATGAGCC AGATAAAGAC TCAGAGTCCT TGACTTCATG TGGCCCATCT 
GGAAAGGGGC AGCAAAGCAA AGCAAAGCCT CCAGAAGCTT TGGATGGGAA ACACGTGGTT 
TCCAGTTCCC CAGTAAGTAG CACCAGTCCT CAGAGCAGAT CGGATGCTGA AATCATAGGA 
GAGACGAAAA CAGCTGAAGA ATTTCCAGCC CATCTCAGCA GCAGTGAAGA AAGCACTGGC 
AGCTGGTCCC AGCTGGCCAA TGATGAGGAC AATCCTGATG ACACCAGCAG CTACCTGCAG 
CTCAGTGAGC GCTCCCTGAG CAATGGCAAC AGCAGTACAA CTAGCAGTCT TGGCATTATG 
GACCTGGAAA TTTATCAGGA GAACATGCCA TCTTCTCCTA TGATTAATGA ATTAGTAGAA 
GAAAAGGTTT TCCTTAAAGA ACAGACAGAA AACACTGAGG GTAAGTTGAA CTATATCAAT 
TCAAACATAC AGTTTGAAGA TAATACAAGC AATTGCACAG TAACTAGCGG GATTATTTTT 
CCAGAAAGTA CTTCTGAGCT TTCAGTGGGA ACAGCCAACT GTCAAAAGGA CCTCCTGGTG 
ATAAACTTTG ATCTGGAACC AGAGTGCCCT GATGTGGAGC TGCGAGCCAC CCTGCAGTGG 
ATTGCTGCTT CTGAACTTGG AATTCCAACT ATCTATTTTA AGAAATCTCA GGAAAACAGA 
ATTGAAAAGT TTCTAGATGT GGTGCAGTTA GTTCAGCGGA AGTCCTGGAA AGTGGGGGAC 
ATCTTCCAAG CCGTGGTGCA GTACTGCAAG CTCAGCGAGG AGGGCAGAGA GATCACACCC 
AGCCTGTTTG ACTGGCTGCT TGAACTGGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279837.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135697.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

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CloneID	OTd51527
Clone ID Related Accession (Same CDS sequence)	XM_030279837.1 , XM_030279839.1
Accession Version	XM_030279837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135697.1
CDS	250..5358
Translation	MHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279837.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279837.1

ORF Insert Sequence:

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279839.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135699.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

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CloneID	OTd51527
Clone ID Related Accession (Same CDS sequence)	XM_030279837.1 , XM_030279839.1
Accession Version	XM_030279839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135699.1
CDS	64..5172
Translation	MHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279839.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279839.1

ORF Insert Sequence:

ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGGTAA GTTGAACTAT ATCAATTCAA ACATACAGTT TGAAGATAAT 
ACAAGCAATT GCACAGTAAC TAGCGGGATT ATTTTTCCAG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279840.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135700.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

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CloneID	OTd51528
Clone ID Related Accession (Same CDS sequence)	XM_030279840.1
Accession Version	XM_030279840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGAAAG TACTTCTGAG CTTTCAGTGG GAACAGCCAA CTGTCAAAAG 
GACCTCCTGG TGATAAACTT TGATCTGGAA CCAGAGTGCC CTGATGTGGA GCTGCGAGCC 
ACCCTGCAGT GGATTGCTGC TTCTGAACTT GGAATTCCAA CTATCTATTT TAAGAAATCT 
CAGGAAAACA GAATTGAAAA GTTTCTAGAT GTGGTGCAGT TAGTTCAGCG GAAGTCCTGG 
AAAGTGGGGG ACATCTTCCA AGCCGTGGTG CAGTACTGCA AGCTCAGCGA GGAGGGCAGA 
GAGATCACAC CCAGCCTGTT TGACTGGCTG CTTGAACTGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135700.1
CDS	222..5306
Translation	MSRPSTGCRAAARSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279840.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279840.1

ORF Insert Sequence:

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGCAAT 
GGCAACAGCA GTACAACTAG CAGTCTTGGC ATTATGGACC TGGAAATTTA TCAGGAGAAC 
ATGCCATCTT CTCCTATGAT TAATGAATTA GTAGAAGAAA AGGTTTTCCT TAAAGAACAG 
ACAGAAAACA CTGAGGAAAG TACTTCTGAG CTTTCAGTGG GAACAGCCAA CTGTCAAAAG 
GACCTCCTGG TGATAAACTT TGATCTGGAA CCAGAGTGCC CTGATGTGGA GCTGCGAGCC 
ACCCTGCAGT GGATTGCTGC TTCTGAACTT GGAATTCCAA CTATCTATTT TAAGAAATCT 
CAGGAAAACA GAATTGAAAA GTTTCTAGAT GTGGTGCAGT TAGTTCAGCG GAAGTCCTGG 
AAAGTGGGGG ACATCTTCCA AGCCGTGGTG CAGTACTGCA AGCTCAGCGA GGAGGGCAGA 
GAGATCACAC CCAGCCTGTT TGACTGGCTG CTTGAACTGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279841.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135701.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTd51529
Clone ID Related Accession (Same CDS sequence)	XM_030279841.1
Accession Version	XM_030279841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGTGAA 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGAAA ACACTGAGGG TAAGTTGAAC 
TATATCAATT CAAACATACA GTTTGAAGAT AATACAAGCA ATTGCACAGT AACTAGCGGG 
ATTATTTTTC CAGAAAGTAC TTCTGAGCTT TCAGTGGGAA CAGCCAACTG TCAAAAGGAC 
CTCCTGGTGA TAAACTTTGA TCTGGAACCA GAGTGCCCTG ATGTGGAGCT GCGAGCCACC 
CTGCAGTGGA TTGCTGCTTC TGAACTTGGA ATTCCAACTA TCTATTTTAA GAAATCTCAG 
GAAAACAGAA TTGAAAAGTT TCTAGATGTG GTGCAGTTAG TTCAGCGGAA GTCCTGGAAA 
GTGGGGGACA TCTTCCAAGC CGTGGTGCAG TACTGCAAGC TCAGCGAGGA GGGCAGAGAG 
ATCACACCCA GCCTGTTTGA CTGGCTGCTT GAACTGGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135701.1
CDS	222..5303
Translation	MSRPSTGCRAAARSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279841.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279841.1

ORF Insert Sequence:

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGTGAA 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGAAA ACACTGAGGG TAAGTTGAAC 
TATATCAATT CAAACATACA GTTTGAAGAT AATACAAGCA ATTGCACAGT AACTAGCGGG 
ATTATTTTTC CAGAAAGTAC TTCTGAGCTT TCAGTGGGAA CAGCCAACTG TCAAAAGGAC 
CTCCTGGTGA TAAACTTTGA TCTGGAACCA GAGTGCCCTG ATGTGGAGCT GCGAGCCACC 
CTGCAGTGGA TTGCTGCTTC TGAACTTGGA ATTCCAACTA TCTATTTTAA GAAATCTCAG 
GAAAACAGAA TTGAAAAGTT TCTAGATGTG GTGCAGTTAG TTCAGCGGAA GTCCTGGAAA 
GTGGGGGACA TCTTCCAAGC CGTGGTGCAG TACTGCAAGC TCAGCGAGGA GGGCAGAGAG 
ATCACACCCA GCCTGTTTGA CTGGCTGCTT GAACTGGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279842.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135702.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X8, mRNA.

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CloneID	OTd51530
Clone ID Related Accession (Same CDS sequence)	XM_030279842.1
Accession Version	XM_030279842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CATGCCATCT 
TCTCCTATGA TTAATGAATT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGAAAAC 
ACTGAGGAAA GTACTTCTGA GCTTTCAGTG GGAACAGCCA ACTGTCAAAA GGACCTCCTG 
GTGATAAACT TTGATCTGGA ACCAGAGTGC CCTGATGTGG AGCTGCGAGC CACCCTGCAG 
TGGATTGCTG CTTCTGAACT TGGAATTCCA ACTATCTATT TTAAGAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTCTAGA TGTGGTGCAG TTAGTTCAGC GGAAGTCCTG GAAAGTGGGG 
GACATCTTCC AAGCCGTGGT GCAGTACTGC AAGCTCAGCG AGGAGGGCAG AGAGATCACA 
CCCAGCCTGT TTGACTGGCT GCTTGAACTG GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135702.1
CDS	93..5168
Translation	MAGRPPPAAPSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279842.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279842.1

ORF Insert Sequence:

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CATGCCATCT 
TCTCCTATGA TTAATGAATT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGAAAAC 
ACTGAGGAAA GTACTTCTGA GCTTTCAGTG GGAACAGCCA ACTGTCAAAA GGACCTCCTG 
GTGATAAACT TTGATCTGGA ACCAGAGTGC CCTGATGTGG AGCTGCGAGC CACCCTGCAG 
TGGATTGCTG CTTCTGAACT TGGAATTCCA ACTATCTATT TTAAGAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTCTAGA TGTGGTGCAG TTAGTTCAGC GGAAGTCCTG GAAAGTGGGG 
GACATCTTCC AAGCCGTGGT GCAGTACTGC AAGCTCAGCG AGGAGGGCAG AGAGATCACA 
CCCAGCCTGT TTGACTGGCT GCTTGAACTG GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279843.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135703.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X9, mRNA.

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CloneID	OTd51531
Clone ID Related Accession (Same CDS sequence)	XM_030279843.1
Accession Version	XM_030279843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGGTTTGT TTTGTGAATT TAGATGCCAA CAGAGATGAT 
TGCAGTGATG AGCAAGTGAA ACAGAAATTG ATCAGCGTCT CACCCAGTCT CCCCAAACTG 
ATCAGTTCGA TGAACGTACA GCCTCCGAAG GAAAATGAAA TAGTACTGCT GAGTGGATTG 
ACATCAGGAA ACCTTCAGGC CGGTTATGAG GTTCCCCAGT GTCCTTGGCT GGCAGATGTC 
TGCTTGGTTC AGTGTGCGAG GGGGGACAGG AGGAACAGCG CAAGCTGCAT TATTTTTGAA 
ATAAACAAGT TTCTGATTGG ACTTGAGCTC GTTCAGGAGA GGCAGCTGCA GATAGAAACT 
CGTGTCCTAA AGCCTGAGGA TGACACAAAC TGCTCCGTGT CCTCAATAGA AGAAGATTTC 
CTCACGGCCT CTGAGCACCT CGAGGATGAC AACGAGGCTG ATGAATATAA AGCTGGTCAT 
GAAAAATTAA ATGCTTCAGA AGCGTCTTCA GATGTCAAAA AAAATAAAGG AAAGGGATTT 
GAAAACATTC ACTATAGAAA AGCCAGGTTG CCGTCCATTC TTGAAGGGAG CTGCATTAAT 
AAAGATAATG CAGCTGCTCA GGTTTCTGAA ACTGTGAATG CTGGGGCTGA ACATGGCATC 
TCACAGCCTG AAGATAAGTC AGACCAGGAA ATACTGTGGC AGAAGGAATT AGCTGAAAAA 
GATACTTCAT CATTTAGTAC TACTAATTTG ACTAGTGGAG CTGAAAACTC GGCACTCATG 
TTAGAAGATG TATCTGTAAC CAAAATGGCT AAAGAGGAGC TGGAGCCTCG GGATGACCCC 
ACAACGAAAC ACAGCAGCAA GACAGATGGG GAAGATTGTG CAGGTGTCAG GAAAACTGAT 
CCCCCCTTCC AGAATGAGCA GGCGACCACA GGTCAGTACG CCACAAATTT AGCAGAATCT 
GTTCTGCAGG ATGCATTCAT TAGACTGTCA CAGTCTCGAC CCAGTTTCAG TGAGGAGGCT 
GCAGTCAGCA TCTCTGCAGG AAGCTCCTGT AAGTCAGAAG ATGTATCTGC TTCCCGATCA 
TGGAACGAAC TCCCAAAGAT CATCATAGTG CAGAGTCCGG ACAGTTCTGA GCACGTACCG 
GACTGGCCAG GCTCTGCCTT CCCCAGCCCC AGCCCCTGGG CTGAGGCAGA AAGCTCTGCT 
GAAGTCTCAG ATTACTTTGA GGAAGAACAT TCAAACGGAC ATGACCAAAG TGCACTGGAA 
GTGGCTCTGG CTTGTGCAGC CACTGTCATT GGAACCATTT CCAGTCCCCA GGCTGTGGAA 
AAATTCCGCT GGGAGCAGGA AGTGGCAGAC TCGAGTGGAG TGGTTGCTAA TGAAGAGGTG 
CAAGCAGCAC CCTCACAGCT CCTTGACGAC TGTGCTGGCA CAGAATACTC GTTCCCATCT 
GCGCTCTGTG GCATGACTCA AGTGGCAAGT GCTGTAGCCA TCTGTGGCCT GGGGGAAACA 
AAGGAGGAGA AATACCCTGC AGCTTCCAGT GGACTTCTGT CTGCTGCTCA GGCCTCTGCA 
GCCATTACTC TGCATTGCAG CTTAGCTGTA GGAAGCAGCA TGGAGAAGCT GAACGAGAGC 
ATTGCAGAGG CGCTTCTGAA AGAGGCATCC CTCATTCTGA CAAAACCTAA CACATACAAA 
AATGTAGGGC ATTTTATGGA ATCCATAAAT GGGAAAATTA TTGAAACAGC AGCAAGACCA 
CATATTCCAC GTGCTGATGG AGTAATCAGG GATGAACTTG CACAAAACTT ATCCAATATT 
ATTCTACGAC ATTCTATAGA AGAAGTTAGG AAGAAGAGAC AGCTACAAGC CCATTCGGAG 
GATGGCTTAA GTACACAAGA CTTTTTCACA GAGACTGCAA ACGAGTTGCT TTTTAATATA 
ATATATTTCA CTTGCAAGAA GATTAACGAC ATAAGGCAAC TCGAAGAGTG TTCTCCTCTC 
TTTTCTGAAA GCGCAAAAGC AGAGAAAGTA ACAAGAGCAG AAGGATGGCC AACCCTGGCA 
ACAGCGTGTG AAACTTCACA CAGCCCCCTT GATCACTCTG TTGCTAAGCC ATTTGGTACA 
TCCTACAGCA CCAGTACTAG TAAAGATCTT GAAAAGGTCA CAAGCACTTG CAAGAGTGAT 
ATCAAAGATG TAAATAGCAA TGAAGGTTCC ACACTGAATT CAGAACCAAG TGTAGATACA 
GGGCATAACA CACTTGGTGC AAAAACATCA CCCAAGAAGA GATACCTGAA AAGAGCTGCA 
CGAGATTGTT ACAAATCCCC AAATCAGAGT AACAATCATC ATCAGAAGAA AGACTACAGA 
TCATTTTCAG ACAGAGAAAA TACCTTTGCA AACAGTGAAT GCAGGCACGG TGTTCAAGAA 
CAGCTGTCTT CCAGTGCCAC CATAAATACA GAAAACCAAG CCAAGATTAA GTGTGATTCT 
GTGCTAAATA ATGATGTCCA ACTTAGCTTG TCTTTGTTAG GAAACCATGT GTTGCTTCCT 
TCTCAGCCTG TGCTACAGGT GAAAAATTCA AGGGACAAAT ATTGTATAAC AGACTTTGCG 
GAAGAATTGG CAGAAACAGT TGTCTCTATG GCAACAGAAA TAGCTGCCAT TTGTCTAGAA 
AATTCAAATG GCAAGCAACC CTGGTTCTGT GCATGGAAGA GAGGCAGTGA ATATCTGATG 
ACCCAGAGTT TATCATGCAG AACCATGAAA AGGAAGAAGG AAACCCATAC CAATGGTTCT 
GTGGTTCGGA AGCACAGGGC ACCTCGGCTT AGTGAGATCA AAAGAAAAAC AGATGAGCAT 
CCTGAGCTAA AGGAAAAATT GATGAATCGA GTAGTAGATG AATCTATAAA CCTTGAGGAC 
ACACCAGATT CAGTCAATCT CTTTGCAAAT GAAGTGGCTG CCAAGATCAT GAACCTCACT 
GAACTCTCCA TGGTTGATAG CATCTGGCAA GGTCCAAACC ACCCCAGGAA CAGACTGCAC 
TGTGAAAGGT GGAGCCGAGC CAAGGCCTCA AGCTGTGAGA GCATACCAGA GGAGGACTCA 
GATTCCAAAG CCTCTTTCAA TACCCTAGGC CTCATGAACA GCTTTGGTCA CTCTCTCAGC 
CAGACAAGTT CTGTCTCAAA GCAGTCTAGT TGTGAGAGCA TTACAGATGA ATTTTCAAGA 
TTTATGGTGA ACCAGATGGA AAACGAAGGA AGAGGATTTG ATTTATTACT GGATTATTAT 
GCAGGAAAAA ATGCAAACAG TATCTTAACT TCTGCCTTGC AACAGGTAGC CAAGAAAAAT 
AGCCATCTTA ATGTAAGACC AAGTTGCCCA TCCAAACAGT CCAGCACAGA AAGTATAACA 
GAAGAGTTTT ATAGGTATAT GCTAAGAGAA ATCGAAAAGG AAAATAAAGA TAATGCATCA 
TCCTCTGGGA ATTCAAAGGA CTGGTGTGGC AGTTTGCTGG CACCCTCTCT ACGGTCACCT 
TTCTGCTTTA GGCAGTCGTC GATGCCTGAC AGTAGATCCT CAGGCTCCAG GCTTACAGTG 
AATGTCCCAA TTAAAGCAAA TTCCTTAGAT GGGTTTGCCC ACCATCGCCA AGATTCACTA 
AGTGTACAGC CCGTCAGTAC CATGGCTTCT GCAGGGCTCT GCAAGTCTGA CTCCTGCCTG 
TACCAGAGAG GCAAGACTGA CCAGATCACA GACATGCTGA TCCACGAGAC CTGGGCCAGT 
TCCATTGAGT CTCTGATGCG CAAGAACAAA ATCATAGCAG ATGAGGCAGA GGCTACAGAG 
GCAGACCAGT TTCACAGTGA TTCTCCACCA CACGTGGAAC AATATGCAAA AAGACTGGCT 
GCAAATATTG TTGAAAGTGG TAAAAATTTA ATTGTTGTCC AGGATTCCTT TGATTATACA 
AGCCGAGAGC ACGTGCTGGC AAGCAAACAT CCCCAAAGCA CAACCCAGGT ACAGCCCAAA 
CCCACAATGG AGGAAGTGAA TTTGGATGAG AAAAAAGAGC ACATGAAGAG CCCTGGAAGC 
CTCCCTGCAG GACAGCTTAG GGAAGTGCCT TTAATTCAGA TAGAAACTGA TCAGCGAGAT 
GAGCCAGATA AAGACTCAGA GTCCTTGACT TCATGTGGCC CATCTGGAAA GGGGCAGCAA 
AGCAAAGCAA AGCCTCCAGA AGCTTTGGAT GGGAAACACG TGGTTTCCAG TTCCCCAGTA 
AGTAGCACCA GTCCTCAGAG CAGATCGGAT GCTGAAATCA TAGGAGAGAC GAAAACAGCT 
GAAGAATTTC CAGCCCATCT CAGCAGCAGT GAAGAAAGCA CTGGCAGCTG GTCCCAGCTG 
GCCAATGATG AGGACAATCC TGATGACACC AGCAGCTACC TGCAGCTCAG TGAGCGCTCC 
CTGAGCAATG GCAACAGCAG TACAACTAGC AGTCTTGGCA TTATGGACCT GGAAATTTAT 
CAGGAGAACA TGCCATCTTC TCCTATGATT AATGAATTAG TAGAAGAAAA GGTTTTCCTT 
AAAGAACAGA CAGAAAACAC TGAGGGTAAG TTGAACTATA TCAATTCAAA CATACAGTTT 
GAAGATAATA CAAGCAATTG CACAGTAACT AGCGGGATTA TTTTTCCAGA AAGTACTTCT 
GAGCTTTCAG TGGGAACAGC CAACTGTCAA AAGGACCTCC TGGTGATAAA CTTTGATCTG 
GAACCAGAGT GCCCTGATGT GGAGCTGCGA GCCACCCTGC AGTGGATTGC TGCTTCTGAA 
CTTGGAATTC CAACTATCTA TTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTGGTGC AGTTAGTTCA GCGGAAGTCC TGGAAAGTGG GGGACATCTT CCAAGCCGTG 
GTGCAGTACT GCAAGCTCAG CGAGGAGGGC AGAGAGATCA CACCCAGCCT GTTTGACTGG 
CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135703.1
CDS	222..5279
Translation	MSRPSTGCRAAARSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENMPSSPMINELVEEKVFLKEQTENTEGKLNYINSNIQFEDNTSNCTVTSGIIFPESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279843.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279843.1

ORF Insert Sequence:

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGGTTTGT TTTGTGAATT TAGATGCCAA CAGAGATGAT 
TGCAGTGATG AGCAAGTGAA ACAGAAATTG ATCAGCGTCT CACCCAGTCT CCCCAAACTG 
ATCAGTTCGA TGAACGTACA GCCTCCGAAG GAAAATGAAA TAGTACTGCT GAGTGGATTG 
ACATCAGGAA ACCTTCAGGC CGGTTATGAG GTTCCCCAGT GTCCTTGGCT GGCAGATGTC 
TGCTTGGTTC AGTGTGCGAG GGGGGACAGG AGGAACAGCG CAAGCTGCAT TATTTTTGAA 
ATAAACAAGT TTCTGATTGG ACTTGAGCTC GTTCAGGAGA GGCAGCTGCA GATAGAAACT 
CGTGTCCTAA AGCCTGAGGA TGACACAAAC TGCTCCGTGT CCTCAATAGA AGAAGATTTC 
CTCACGGCCT CTGAGCACCT CGAGGATGAC AACGAGGCTG ATGAATATAA AGCTGGTCAT 
GAAAAATTAA ATGCTTCAGA AGCGTCTTCA GATGTCAAAA AAAATAAAGG AAAGGGATTT 
GAAAACATTC ACTATAGAAA AGCCAGGTTG CCGTCCATTC TTGAAGGGAG CTGCATTAAT 
AAAGATAATG CAGCTGCTCA GGTTTCTGAA ACTGTGAATG CTGGGGCTGA ACATGGCATC 
TCACAGCCTG AAGATAAGTC AGACCAGGAA ATACTGTGGC AGAAGGAATT AGCTGAAAAA 
GATACTTCAT CATTTAGTAC TACTAATTTG ACTAGTGGAG CTGAAAACTC GGCACTCATG 
TTAGAAGATG TATCTGTAAC CAAAATGGCT AAAGAGGAGC TGGAGCCTCG GGATGACCCC 
ACAACGAAAC ACAGCAGCAA GACAGATGGG GAAGATTGTG CAGGTGTCAG GAAAACTGAT 
CCCCCCTTCC AGAATGAGCA GGCGACCACA GGTCAGTACG CCACAAATTT AGCAGAATCT 
GTTCTGCAGG ATGCATTCAT TAGACTGTCA CAGTCTCGAC CCAGTTTCAG TGAGGAGGCT 
GCAGTCAGCA TCTCTGCAGG AAGCTCCTGT AAGTCAGAAG ATGTATCTGC TTCCCGATCA 
TGGAACGAAC TCCCAAAGAT CATCATAGTG CAGAGTCCGG ACAGTTCTGA GCACGTACCG 
GACTGGCCAG GCTCTGCCTT CCCCAGCCCC AGCCCCTGGG CTGAGGCAGA AAGCTCTGCT 
GAAGTCTCAG ATTACTTTGA GGAAGAACAT TCAAACGGAC ATGACCAAAG TGCACTGGAA 
GTGGCTCTGG CTTGTGCAGC CACTGTCATT GGAACCATTT CCAGTCCCCA GGCTGTGGAA 
AAATTCCGCT GGGAGCAGGA AGTGGCAGAC TCGAGTGGAG TGGTTGCTAA TGAAGAGGTG 
CAAGCAGCAC CCTCACAGCT CCTTGACGAC TGTGCTGGCA CAGAATACTC GTTCCCATCT 
GCGCTCTGTG GCATGACTCA AGTGGCAAGT GCTGTAGCCA TCTGTGGCCT GGGGGAAACA 
AAGGAGGAGA AATACCCTGC AGCTTCCAGT GGACTTCTGT CTGCTGCTCA GGCCTCTGCA 
GCCATTACTC TGCATTGCAG CTTAGCTGTA GGAAGCAGCA TGGAGAAGCT GAACGAGAGC 
ATTGCAGAGG CGCTTCTGAA AGAGGCATCC CTCATTCTGA CAAAACCTAA CACATACAAA 
AATGTAGGGC ATTTTATGGA ATCCATAAAT GGGAAAATTA TTGAAACAGC AGCAAGACCA 
CATATTCCAC GTGCTGATGG AGTAATCAGG GATGAACTTG CACAAAACTT ATCCAATATT 
ATTCTACGAC ATTCTATAGA AGAAGTTAGG AAGAAGAGAC AGCTACAAGC CCATTCGGAG 
GATGGCTTAA GTACACAAGA CTTTTTCACA GAGACTGCAA ACGAGTTGCT TTTTAATATA 
ATATATTTCA CTTGCAAGAA GATTAACGAC ATAAGGCAAC TCGAAGAGTG TTCTCCTCTC 
TTTTCTGAAA GCGCAAAAGC AGAGAAAGTA ACAAGAGCAG AAGGATGGCC AACCCTGGCA 
ACAGCGTGTG AAACTTCACA CAGCCCCCTT GATCACTCTG TTGCTAAGCC ATTTGGTACA 
TCCTACAGCA CCAGTACTAG TAAAGATCTT GAAAAGGTCA CAAGCACTTG CAAGAGTGAT 
ATCAAAGATG TAAATAGCAA TGAAGGTTCC ACACTGAATT CAGAACCAAG TGTAGATACA 
GGGCATAACA CACTTGGTGC AAAAACATCA CCCAAGAAGA GATACCTGAA AAGAGCTGCA 
CGAGATTGTT ACAAATCCCC AAATCAGAGT AACAATCATC ATCAGAAGAA AGACTACAGA 
TCATTTTCAG ACAGAGAAAA TACCTTTGCA AACAGTGAAT GCAGGCACGG TGTTCAAGAA 
CAGCTGTCTT CCAGTGCCAC CATAAATACA GAAAACCAAG CCAAGATTAA GTGTGATTCT 
GTGCTAAATA ATGATGTCCA ACTTAGCTTG TCTTTGTTAG GAAACCATGT GTTGCTTCCT 
TCTCAGCCTG TGCTACAGGT GAAAAATTCA AGGGACAAAT ATTGTATAAC AGACTTTGCG 
GAAGAATTGG CAGAAACAGT TGTCTCTATG GCAACAGAAA TAGCTGCCAT TTGTCTAGAA 
AATTCAAATG GCAAGCAACC CTGGTTCTGT GCATGGAAGA GAGGCAGTGA ATATCTGATG 
ACCCAGAGTT TATCATGCAG AACCATGAAA AGGAAGAAGG AAACCCATAC CAATGGTTCT 
GTGGTTCGGA AGCACAGGGC ACCTCGGCTT AGTGAGATCA AAAGAAAAAC AGATGAGCAT 
CCTGAGCTAA AGGAAAAATT GATGAATCGA GTAGTAGATG AATCTATAAA CCTTGAGGAC 
ACACCAGATT CAGTCAATCT CTTTGCAAAT GAAGTGGCTG CCAAGATCAT GAACCTCACT 
GAACTCTCCA TGGTTGATAG CATCTGGCAA GGTCCAAACC ACCCCAGGAA CAGACTGCAC 
TGTGAAAGGT GGAGCCGAGC CAAGGCCTCA AGCTGTGAGA GCATACCAGA GGAGGACTCA 
GATTCCAAAG CCTCTTTCAA TACCCTAGGC CTCATGAACA GCTTTGGTCA CTCTCTCAGC 
CAGACAAGTT CTGTCTCAAA GCAGTCTAGT TGTGAGAGCA TTACAGATGA ATTTTCAAGA 
TTTATGGTGA ACCAGATGGA AAACGAAGGA AGAGGATTTG ATTTATTACT GGATTATTAT 
GCAGGAAAAA ATGCAAACAG TATCTTAACT TCTGCCTTGC AACAGGTAGC CAAGAAAAAT 
AGCCATCTTA ATGTAAGACC AAGTTGCCCA TCCAAACAGT CCAGCACAGA AAGTATAACA 
GAAGAGTTTT ATAGGTATAT GCTAAGAGAA ATCGAAAAGG AAAATAAAGA TAATGCATCA 
TCCTCTGGGA ATTCAAAGGA CTGGTGTGGC AGTTTGCTGG CACCCTCTCT ACGGTCACCT 
TTCTGCTTTA GGCAGTCGTC GATGCCTGAC AGTAGATCCT CAGGCTCCAG GCTTACAGTG 
AATGTCCCAA TTAAAGCAAA TTCCTTAGAT GGGTTTGCCC ACCATCGCCA AGATTCACTA 
AGTGTACAGC CCGTCAGTAC CATGGCTTCT GCAGGGCTCT GCAAGTCTGA CTCCTGCCTG 
TACCAGAGAG GCAAGACTGA CCAGATCACA GACATGCTGA TCCACGAGAC CTGGGCCAGT 
TCCATTGAGT CTCTGATGCG CAAGAACAAA ATCATAGCAG ATGAGGCAGA GGCTACAGAG 
GCAGACCAGT TTCACAGTGA TTCTCCACCA CACGTGGAAC AATATGCAAA AAGACTGGCT 
GCAAATATTG TTGAAAGTGG TAAAAATTTA ATTGTTGTCC AGGATTCCTT TGATTATACA 
AGCCGAGAGC ACGTGCTGGC AAGCAAACAT CCCCAAAGCA CAACCCAGGT ACAGCCCAAA 
CCCACAATGG AGGAAGTGAA TTTGGATGAG AAAAAAGAGC ACATGAAGAG CCCTGGAAGC 
CTCCCTGCAG GACAGCTTAG GGAAGTGCCT TTAATTCAGA TAGAAACTGA TCAGCGAGAT 
GAGCCAGATA AAGACTCAGA GTCCTTGACT TCATGTGGCC CATCTGGAAA GGGGCAGCAA 
AGCAAAGCAA AGCCTCCAGA AGCTTTGGAT GGGAAACACG TGGTTTCCAG TTCCCCAGTA 
AGTAGCACCA GTCCTCAGAG CAGATCGGAT GCTGAAATCA TAGGAGAGAC GAAAACAGCT 
GAAGAATTTC CAGCCCATCT CAGCAGCAGT GAAGAAAGCA CTGGCAGCTG GTCCCAGCTG 
GCCAATGATG AGGACAATCC TGATGACACC AGCAGCTACC TGCAGCTCAG TGAGCGCTCC 
CTGAGCAATG GCAACAGCAG TACAACTAGC AGTCTTGGCA TTATGGACCT GGAAATTTAT 
CAGGAGAACA TGCCATCTTC TCCTATGATT AATGAATTAG TAGAAGAAAA GGTTTTCCTT 
AAAGAACAGA CAGAAAACAC TGAGGGTAAG TTGAACTATA TCAATTCAAA CATACAGTTT 
GAAGATAATA CAAGCAATTG CACAGTAACT AGCGGGATTA TTTTTCCAGA AAGTACTTCT 
GAGCTTTCAG TGGGAACAGC CAACTGTCAA AAGGACCTCC TGGTGATAAA CTTTGATCTG 
GAACCAGAGT GCCCTGATGT GGAGCTGCGA GCCACCCTGC AGTGGATTGC TGCTTCTGAA 
CTTGGAATTC CAACTATCTA TTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTGGTGC AGTTAGTTCA GCGGAAGTCC TGGAAAGTGG GGGACATCTT CCAAGCCGTG 
GTGCAGTACT GCAAGCTCAG CGAGGAGGGC AGAGAGATCA CACCCAGCCT GTTTGACTGG 
CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279844.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135704.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X10, mRNA.

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CloneID	OTd51532
Clone ID Related Accession (Same CDS sequence)	XM_030279844.1
Accession Version	XM_030279844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGTGAA 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGAAA ACACTGAGGA AAGTACTTCT 
GAGCTTTCAG TGGGAACAGC CAACTGTCAA AAGGACCTCC TGGTGATAAA CTTTGATCTG 
GAACCAGAGT GCCCTGATGT GGAGCTGCGA GCCACCCTGC AGTGGATTGC TGCTTCTGAA 
CTTGGAATTC CAACTATCTA TTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTGGTGC AGTTAGTTCA GCGGAAGTCC TGGAAAGTGG GGGACATCTT CCAAGCCGTG 
GTGCAGTACT GCAAGCTCAG CGAGGAGGGC AGAGAGATCA CACCCAGCCT GTTTGACTGG 
CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135704.1
CDS	222..5219
Translation	MSRPSTGCRAAARSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279844.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279844.1

ORF Insert Sequence:

ATGTCCCGGC CGAGCACCGG CTGCCGGGCA GCGGCTCGCA GTAACTTTGA ATCTCCACTG 
ATGCATGAAG TCCCAGAGCG CCAAGGCAGC AGCACAGACA GTTCTGCAAG CAGTTTTGGA 
AGCTCCGTCA CAGCATGTAA GAAGATTCTC TGCAGTAACA GTCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTAGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCCA ACAGAGATGA TTGCAGTGAT 
GAGCAAGTGA AACAGAAATT GATCAGCGTC TCACCCAGTC TCCCCAAACT GATCAGTTCG 
ATGAACGTAC AGCCTCCGAA GGAAAATGAA ATAGTACTGC TGAGTGGATT GACATCAGGA 
AACCTTCAGG CCGGTTATGA GGTTCCCCAG TGTCCTTGGC TGGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGGGACAG GAGGAACAGC GCAAGCTGCA TTATTTTTGA AATAAACAAG 
TTTCTGATTG GACTTGAGCT CGTTCAGGAG AGGCAGCTGC AGATAGAAAC TCGTGTCCTA 
AAGCCTGAGG ATGACACAAA CTGCTCCGTG TCCTCAATAG AAGAAGATTT CCTCACGGCC 
TCTGAGCACC TCGAGGATGA CAACGAGGCT GATGAATATA AAGCTGGTCA TGAAAAATTA 
AATGCTTCAG AAGCGTCTTC AGATGTCAAA AAAAATAAAG GAAAGGGATT TGAAAACATT 
CACTATAGAA AAGCCAGGTT GCCGTCCATT CTTGAAGGGA GCTGCATTAA TAAAGATAAT 
GCAGCTGCTC AGGTTTCTGA AACTGTGAAT GCTGGGGCTG AACATGGCAT CTCACAGCCT 
GAAGATAAGT CAGACCAGGA AATACTGTGG CAGAAGGAAT TAGCTGAAAA AGATACTTCA 
TCATTTAGTA CTACTAATTT GACTAGTGGA GCTGAAAACT CGGCACTCAT GTTAGAAGAT 
GTATCTGTAA CCAAAATGGC TAAAGAGGAG CTGGAGCCTC GGGATGACCC CACAACGAAA 
CACAGCAGCA AGACAGATGG GGAAGATTGT GCAGGTGTCA GGAAAACTGA TCCCCCCTTC 
CAGAATGAGC AGGCGACCAC AGGTCAGTAC GCCACAAATT TAGCAGAATC TGTTCTGCAG 
GATGCATTCA TTAGACTGTC ACAGTCTCGA CCCAGTTTCA GTGAGGAGGC TGCAGTCAGC 
ATCTCTGCAG GAAGCTCCTG TAAGTCAGAA GATGTATCTG CTTCCCGATC ATGGAACGAA 
CTCCCAAAGA TCATCATAGT GCAGAGTCCG GACAGTTCTG AGCACGTACC GGACTGGCCA 
GGCTCTGCCT TCCCCAGCCC CAGCCCCTGG GCTGAGGCAG AAAGCTCTGC TGAAGTCTCA 
GATTACTTTG AGGAAGAACA TTCAAACGGA CATGACCAAA GTGCACTGGA AGTGGCTCTG 
GCTTGTGCAG CCACTGTCAT TGGAACCATT TCCAGTCCCC AGGCTGTGGA AAAATTCCGC 
TGGGAGCAGG AAGTGGCAGA CTCGAGTGGA GTGGTTGCTA ATGAAGAGGT GCAAGCAGCA 
CCCTCACAGC TCCTTGACGA CTGTGCTGGC ACAGAATACT CGTTCCCATC TGCGCTCTGT 
GGCATGACTC AAGTGGCAAG TGCTGTAGCC ATCTGTGGCC TGGGGGAAAC AAAGGAGGAG 
AAATACCCTG CAGCTTCCAG TGGACTTCTG TCTGCTGCTC AGGCCTCTGC AGCCATTACT 
CTGCATTGCA GCTTAGCTGT AGGAAGCAGC ATGGAGAAGC TGAACGAGAG CATTGCAGAG 
GCGCTTCTGA AAGAGGCATC CCTCATTCTG ACAAAACCTA ACACATACAA AAATGTAGGG 
CATTTTATGG AATCCATAAA TGGGAAAATT ATTGAAACAG CAGCAAGACC ACATATTCCA 
CGTGCTGATG GAGTAATCAG GGATGAACTT GCACAAAACT TATCCAATAT TATTCTACGA 
CATTCTATAG AAGAAGTTAG GAAGAAGAGA CAGCTACAAG CCCATTCGGA GGATGGCTTA 
AGTACACAAG ACTTTTTCAC AGAGACTGCA AACGAGTTGC TTTTTAATAT AATATATTTC 
ACTTGCAAGA AGATTAACGA CATAAGGCAA CTCGAAGAGT GTTCTCCTCT CTTTTCTGAA 
AGCGCAAAAG CAGAGAAAGT AACAAGAGCA GAAGGATGGC CAACCCTGGC AACAGCGTGT 
GAAACTTCAC ACAGCCCCCT TGATCACTCT GTTGCTAAGC CATTTGGTAC ATCCTACAGC 
ACCAGTACTA GTAAAGATCT TGAAAAGGTC ACAAGCACTT GCAAGAGTGA TATCAAAGAT 
GTAAATAGCA ATGAAGGTTC CACACTGAAT TCAGAACCAA GTGTAGATAC AGGGCATAAC 
ACACTTGGTG CAAAAACATC ACCCAAGAAG AGATACCTGA AAAGAGCTGC ACGAGATTGT 
TACAAATCCC CAAATCAGAG TAACAATCAT CATCAGAAGA AAGACTACAG ATCATTTTCA 
GACAGAGAAA ATACCTTTGC AAACAGTGAA TGCAGGCACG GTGTTCAAGA ACAGCTGTCT 
TCCAGTGCCA CCATAAATAC AGAAAACCAA GCCAAGATTA AGTGTGATTC TGTGCTAAAT 
AATGATGTCC AACTTAGCTT GTCTTTGTTA GGAAACCATG TGTTGCTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGGGACAAA TATTGTATAA CAGACTTTGC GGAAGAATTG 
GCAGAAACAG TTGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTAGA AAATTCAAAT 
GGCAAGCAAC CCTGGTTCTG TGCATGGAAG AGAGGCAGTG AATATCTGAT GACCCAGAGT 
TTATCATGCA GAACCATGAA AAGGAAGAAG GAAACCCATA CCAATGGTTC TGTGGTTCGG 
AAGCACAGGG CACCTCGGCT TAGTGAGATC AAAAGAAAAA CAGATGAGCA TCCTGAGCTA 
AAGGAAAAAT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCAGTCAATC TCTTTGCAAA TGAAGTGGCT GCCAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGATA GCATCTGGCA AGGTCCAAAC CACCCCAGGA ACAGACTGCA CTGTGAAAGG 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACTC AGATTCCAAA 
GCCTCTTTCA ATACCCTAGG CCTCATGAAC AGCTTTGGTC ACTCTCTCAG CCAGACAAGT 
TCTGTCTCAA AGCAGTCTAG TTGTGAGAGC ATTACAGATG AATTTTCAAG ATTTATGGTG 
AACCAGATGG AAAACGAAGG AAGAGGATTT GATTTATTAC TGGATTATTA TGCAGGAAAA 
AATGCAAACA GTATCTTAAC TTCTGCCTTG CAACAGGTAG CCAAGAAAAA TAGCCATCTT 
AATGTAAGAC CAAGTTGCCC ATCCAAACAG TCCAGCACAG AAAGTATAAC AGAAGAGTTT 
TATAGGTATA TGCTAAGAGA AATCGAAAAG GAAAATAAAG ATAATGCATC ATCCTCTGGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTG GCACCCTCTC TACGGTCACC TTTCTGCTTT 
AGGCAGTCGT CGATGCCTGA CAGTAGATCC TCAGGCTCCA GGCTTACAGT GAATGTCCCA 
ATTAAAGCAA ATTCCTTAGA TGGGTTTGCC CACCATCGCC AAGATTCACT AAGTGTACAG 
CCCGTCAGTA CCATGGCTTC TGCAGGGCTC TGCAAGTCTG ACTCCTGCCT GTACCAGAGA 
GGCAAGACTG ACCAGATCAC AGACATGCTG ATCCACGAGA CCTGGGCCAG TTCCATTGAG 
TCTCTGATGC GCAAGAACAA AATCATAGCA GATGAGGCAG AGGCTACAGA GGCAGACCAG 
TTTCACAGTG ATTCTCCACC ACACGTGGAA CAATATGCAA AAAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGGATTCCT TTGATTATAC AAGCCGAGAG 
CACGTGCTGG CAAGCAAACA TCCCCAAAGC ACAACCCAGG TACAGCCCAA ACCCACAATG 
GAGGAAGTGA ATTTGGATGA GAAAAAAGAG CACATGAAGA GCCCTGGAAG CCTCCCTGCA 
GGACAGCTTA GGGAAGTGCC TTTAATTCAG ATAGAAACTG ATCAGCGAGA TGAGCCAGAT 
AAAGACTCAG AGTCCTTGAC TTCATGTGGC CCATCTGGAA AGGGGCAGCA AAGCAAAGCA 
AAGCCTCCAG AAGCTTTGGA TGGGAAACAC GTGGTTTCCA GTTCCCCAGT AAGTAGCACC 
AGTCCTCAGA GCAGATCGGA TGCTGAAATC ATAGGAGAGA CGAAAACAGC TGAAGAATTT 
CCAGCCCATC TCAGCAGCAG TGAAGAAAGC ACTGGCAGCT GGTCCCAGCT GGCCAATGAT 
GAGGACAATC CTGATGACAC CAGCAGCTAC CTGCAGCTCA GTGAGCGCTC CCTGAGTGAA 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGAAA ACACTGAGGA AAGTACTTCT 
GAGCTTTCAG TGGGAACAGC CAACTGTCAA AAGGACCTCC TGGTGATAAA CTTTGATCTG 
GAACCAGAGT GCCCTGATGT GGAGCTGCGA GCCACCCTGC AGTGGATTGC TGCTTCTGAA 
CTTGGAATTC CAACTATCTA TTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTGGTGC AGTTAGTTCA GCGGAAGTCC TGGAAAGTGG GGGACATCTT CCAAGCCGTG 
GTGCAGTACT GCAAGCTCAG CGAGGAGGGC AGAGAGATCA CACCCAGCCT GTTTGACTGG 
CTGCTTGAAC TGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_030279845.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030135705.1 Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X11, mRNA.

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CloneID	OTd51533
Clone ID Related Accession (Same CDS sequence)	XM_030279845.1
Accession Version	XM_030279845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	A-kinase anchor protein SPHKAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044221.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGTGA ATTAGTAGAA 
GAAAAGGTTT TCCTTAAAGA ACAGACAGAA AACACTGAGG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030135705.1
CDS	94..5082
Translation	MAGRPPPAAPSNFESPLMHEVPERQGSSTDSSASSFGSSVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSDEQVKQKLISVSPSLPKLISSMNVQPPKENEIVLLSGLTSGNLQAGYEVPQCPWLADVCLVQCARGDRRNSASCIIFEINKFLIGLELVQERQLQIETRVLKPEDDTNCSVSSIEEDFLTASEHLEDDNEADEYKAGHEKLNASEASSDVKKNKGKGFENIHYRKARLPSILEGSCINKDNAAAQVSETVNAGAEHGISQPEDKSDQEILWQKELAEKDTSSFSTTNLTSGAENSALMLEDVSVTKMAKEELEPRDDPTTKHSSKTDGEDCAGVRKTDPPFQNEQATTGQYATNLAESVLQDAFIRLSQSRPSFSEEAAVSISAGSSCKSEDVSASRSWNELPKIIIVQSPDSSEHVPDWPGSAFPSPSPWAEAESSAEVSDYFEEEHSNGHDQSALEVALACAATVIGTISSPQAVEKFRWEQEVADSSGVVANEEVQAAPSQLLDDCAGTEYSFPSALCGMTQVASAVAICGLGETKEEKYPAASSGLLSAAQASAAITLHCSLAVGSSMEKLNESIAEALLKEASLILTKPNTYKNVGHFMESINGKIIETAARPHIPRADGVIRDELAQNLSNIILRHSIEEVRKKRQLQAHSEDGLSTQDFFTETANELLFNIIYFTCKKINDIRQLEECSPLFSESAKAEKVTRAEGWPTLATACETSHSPLDHSVAKPFGTSYSTSTSKDLEKVTSTCKSDIKDVNSNEGSTLNSEPSVDTGHNTLGAKTSPKKRYLKRAARDCYKSPNQSNNHHQKKDYRSFSDRENTFANSECRHGVQEQLSSSATINTENQAKIKCDSVLNNDVQLSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGSEYLMTQSLSCRTMKRKKETHTNGSVVRKHRAPRLSEIKRKTDEHPELKEKLMNRVVDESINLEDTPDSVNLFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDSDSKASFNTLGLMNSFGHSLSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANSILTSALQQVAKKNSHLNVRPSCPSKQSSTESITEEFYRYMLREIEKENKDNASSSGNSKDWCGSLLAPSLRSPFCFRQSSMPDSRSSGSRLTVNVPIKANSLDGFAHHRQDSLSVQPVSTMASAGLCKSDSCLYQRGKTDQITDMLIHETWASSIESLMRKNKIIADEAEATEADQFHSDSPPHVEQYAKRLAANIVESGKNLIVVQDSFDYTSREHVLASKHPQSTTQVQPKPTMEEVNLDEKKEHMKSPGSLPAGQLREVPLIQIETDQRDEPDKDSESLTSCGPSGKGQQSKAKPPEALDGKHVVSSSPVSSTSPQSRSDAEIIGETKTAEEFPAHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSELVEEKVFLKEQTENTEESTSELSVGTANCQKDLLVINFDLEPECPDVELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFQAVVQYCKLSEEGREITPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_030279845.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030279845.1

ORF Insert Sequence:

ATGGCCGGGC GCCCCCCGCC CGCCGCCCCC AGTAACTTTG AATCTCCACT GATGCATGAA 
GTCCCAGAGC GCCAAGGCAG CAGCACAGAC AGTTCTGCAA GCAGTTTTGG AAGCTCCGTC 
ACAGCATGTA AGAAGATTCT CTGCAGTAAC AGTCTGCTAG AATCAACAGA CTACTGGCTG 
CAGAATCAGA GGACACCATG CCAAATTGGC TTTCTAGAAG ACAAGTCAGA AAGCAACTGT 
GCCTCAGTTT GTTTTGTGAA TTTAGATGCC AACAGAGATG ATTGCAGTGA TGAGCAAGTG 
AAACAGAAAT TGATCAGCGT CTCACCCAGT CTCCCCAAAC TGATCAGTTC GATGAACGTA 
CAGCCTCCGA AGGAAAATGA AATAGTACTG CTGAGTGGAT TGACATCAGG AAACCTTCAG 
GCCGGTTATG AGGTTCCCCA GTGTCCTTGG CTGGCAGATG TCTGCTTGGT TCAGTGTGCG 
AGGGGGGACA GGAGGAACAG CGCAAGCTGC ATTATTTTTG AAATAAACAA GTTTCTGATT 
GGACTTGAGC TCGTTCAGGA GAGGCAGCTG CAGATAGAAA CTCGTGTCCT AAAGCCTGAG 
GATGACACAA ACTGCTCCGT GTCCTCAATA GAAGAAGATT TCCTCACGGC CTCTGAGCAC 
CTCGAGGATG ACAACGAGGC TGATGAATAT AAAGCTGGTC ATGAAAAATT AAATGCTTCA 
GAAGCGTCTT CAGATGTCAA AAAAAATAAA GGAAAGGGAT TTGAAAACAT TCACTATAGA 
AAAGCCAGGT TGCCGTCCAT TCTTGAAGGG AGCTGCATTA ATAAAGATAA TGCAGCTGCT 
CAGGTTTCTG AAACTGTGAA TGCTGGGGCT GAACATGGCA TCTCACAGCC TGAAGATAAG 
TCAGACCAGG AAATACTGTG GCAGAAGGAA TTAGCTGAAA AAGATACTTC ATCATTTAGT 
ACTACTAATT TGACTAGTGG AGCTGAAAAC TCGGCACTCA TGTTAGAAGA TGTATCTGTA 
ACCAAAATGG CTAAAGAGGA GCTGGAGCCT CGGGATGACC CCACAACGAA ACACAGCAGC 
AAGACAGATG GGGAAGATTG TGCAGGTGTC AGGAAAACTG ATCCCCCCTT CCAGAATGAG 
CAGGCGACCA CAGGTCAGTA CGCCACAAAT TTAGCAGAAT CTGTTCTGCA GGATGCATTC 
ATTAGACTGT CACAGTCTCG ACCCAGTTTC AGTGAGGAGG CTGCAGTCAG CATCTCTGCA 
GGAAGCTCCT GTAAGTCAGA AGATGTATCT GCTTCCCGAT CATGGAACGA ACTCCCAAAG 
ATCATCATAG TGCAGAGTCC GGACAGTTCT GAGCACGTAC CGGACTGGCC AGGCTCTGCC 
TTCCCCAGCC CCAGCCCCTG GGCTGAGGCA GAAAGCTCTG CTGAAGTCTC AGATTACTTT 
GAGGAAGAAC ATTCAAACGG ACATGACCAA AGTGCACTGG AAGTGGCTCT GGCTTGTGCA 
GCCACTGTCA TTGGAACCAT TTCCAGTCCC CAGGCTGTGG AAAAATTCCG CTGGGAGCAG 
GAAGTGGCAG ACTCGAGTGG AGTGGTTGCT AATGAAGAGG TGCAAGCAGC ACCCTCACAG 
CTCCTTGACG ACTGTGCTGG CACAGAATAC TCGTTCCCAT CTGCGCTCTG TGGCATGACT 
CAAGTGGCAA GTGCTGTAGC CATCTGTGGC CTGGGGGAAA CAAAGGAGGA GAAATACCCT 
GCAGCTTCCA GTGGACTTCT GTCTGCTGCT CAGGCCTCTG CAGCCATTAC TCTGCATTGC 
AGCTTAGCTG TAGGAAGCAG CATGGAGAAG CTGAACGAGA GCATTGCAGA GGCGCTTCTG 
AAAGAGGCAT CCCTCATTCT GACAAAACCT AACACATACA AAAATGTAGG GCATTTTATG 
GAATCCATAA ATGGGAAAAT TATTGAAACA GCAGCAAGAC CACATATTCC ACGTGCTGAT 
GGAGTAATCA GGGATGAACT TGCACAAAAC TTATCCAATA TTATTCTACG ACATTCTATA 
GAAGAAGTTA GGAAGAAGAG ACAGCTACAA GCCCATTCGG AGGATGGCTT AAGTACACAA 
GACTTTTTCA CAGAGACTGC AAACGAGTTG CTTTTTAATA TAATATATTT CACTTGCAAG 
AAGATTAACG ACATAAGGCA ACTCGAAGAG TGTTCTCCTC TCTTTTCTGA AAGCGCAAAA 
GCAGAGAAAG TAACAAGAGC AGAAGGATGG CCAACCCTGG CAACAGCGTG TGAAACTTCA 
CACAGCCCCC TTGATCACTC TGTTGCTAAG CCATTTGGTA CATCCTACAG CACCAGTACT 
AGTAAAGATC TTGAAAAGGT CACAAGCACT TGCAAGAGTG ATATCAAAGA TGTAAATAGC 
AATGAAGGTT CCACACTGAA TTCAGAACCA AGTGTAGATA CAGGGCATAA CACACTTGGT 
GCAAAAACAT CACCCAAGAA GAGATACCTG AAAAGAGCTG CACGAGATTG TTACAAATCC 
CCAAATCAGA GTAACAATCA TCATCAGAAG AAAGACTACA GATCATTTTC AGACAGAGAA 
AATACCTTTG CAAACAGTGA ATGCAGGCAC GGTGTTCAAG AACAGCTGTC TTCCAGTGCC 
ACCATAAATA CAGAAAACCA AGCCAAGATT AAGTGTGATT CTGTGCTAAA TAATGATGTC 
CAACTTAGCT TGTCTTTGTT AGGAAACCAT GTGTTGCTTC CTTCTCAGCC TGTGCTACAG 
GTGAAAAATT CAAGGGACAA ATATTGTATA ACAGACTTTG CGGAAGAATT GGCAGAAACA 
GTTGTCTCTA TGGCAACAGA AATAGCTGCC ATTTGTCTAG AAAATTCAAA TGGCAAGCAA 
CCCTGGTTCT GTGCATGGAA GAGAGGCAGT GAATATCTGA TGACCCAGAG TTTATCATGC 
AGAACCATGA AAAGGAAGAA GGAAACCCAT ACCAATGGTT CTGTGGTTCG GAAGCACAGG 
GCACCTCGGC TTAGTGAGAT CAAAAGAAAA ACAGATGAGC ATCCTGAGCT AAAGGAAAAA 
TTGATGAATC GAGTAGTAGA TGAATCTATA AACCTTGAGG ACACACCAGA TTCAGTCAAT 
CTCTTTGCAA ATGAAGTGGC TGCCAAGATC ATGAACCTCA CTGAACTCTC CATGGTTGAT 
AGCATCTGGC AAGGTCCAAA CCACCCCAGG AACAGACTGC ACTGTGAAAG GTGGAGCCGA 
GCCAAGGCCT CAAGCTGTGA GAGCATACCA GAGGAGGACT CAGATTCCAA AGCCTCTTTC 
AATACCCTAG GCCTCATGAA CAGCTTTGGT CACTCTCTCA GCCAGACAAG TTCTGTCTCA 
AAGCAGTCTA GTTGTGAGAG CATTACAGAT GAATTTTCAA GATTTATGGT GAACCAGATG 
GAAAACGAAG GAAGAGGATT TGATTTATTA CTGGATTATT ATGCAGGAAA AAATGCAAAC 
AGTATCTTAA CTTCTGCCTT GCAACAGGTA GCCAAGAAAA ATAGCCATCT TAATGTAAGA 
CCAAGTTGCC CATCCAAACA GTCCAGCACA GAAAGTATAA CAGAAGAGTT TTATAGGTAT 
ATGCTAAGAG AAATCGAAAA GGAAAATAAA GATAATGCAT CATCCTCTGG GAATTCAAAG 
GACTGGTGTG GCAGTTTGCT GGCACCCTCT CTACGGTCAC CTTTCTGCTT TAGGCAGTCG 
TCGATGCCTG ACAGTAGATC CTCAGGCTCC AGGCTTACAG TGAATGTCCC AATTAAAGCA 
AATTCCTTAG ATGGGTTTGC CCACCATCGC CAAGATTCAC TAAGTGTACA GCCCGTCAGT 
ACCATGGCTT CTGCAGGGCT CTGCAAGTCT GACTCCTGCC TGTACCAGAG AGGCAAGACT 
GACCAGATCA CAGACATGCT GATCCACGAG ACCTGGGCCA GTTCCATTGA GTCTCTGATG 
CGCAAGAACA AAATCATAGC AGATGAGGCA GAGGCTACAG AGGCAGACCA GTTTCACAGT 
GATTCTCCAC CACACGTGGA ACAATATGCA AAAAGACTGG CTGCAAATAT TGTTGAAAGT 
GGTAAAAATT TAATTGTTGT CCAGGATTCC TTTGATTATA CAAGCCGAGA GCACGTGCTG 
GCAAGCAAAC ATCCCCAAAG CACAACCCAG GTACAGCCCA AACCCACAAT GGAGGAAGTG 
AATTTGGATG AGAAAAAAGA GCACATGAAG AGCCCTGGAA GCCTCCCTGC AGGACAGCTT 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAGCGAG ATGAGCCAGA TAAAGACTCA 
GAGTCCTTGA CTTCATGTGG CCCATCTGGA AAGGGGCAGC AAAGCAAAGC AAAGCCTCCA 
GAAGCTTTGG ATGGGAAACA CGTGGTTTCC AGTTCCCCAG TAAGTAGCAC CAGTCCTCAG 
AGCAGATCGG ATGCTGAAAT CATAGGAGAG ACGAAAACAG CTGAAGAATT TCCAGCCCAT 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TGGCCAATGA TGAGGACAAT 
CCTGATGACA CCAGCAGCTA CCTGCAGCTC AGTGAGCGCT CCCTGAGTGA ATTAGTAGAA 
GAAAAGGTTT TCCTTAAAGA ACAGACAGAA AACACTGAGG AAAGTACTTC TGAGCTTTCA 
GTGGGAACAG CCAACTGTCA AAAGGACCTC CTGGTGATAA ACTTTGATCT GGAACCAGAG 
TGCCCTGATG TGGAGCTGCG AGCCACCCTG CAGTGGATTG CTGCTTCTGA ACTTGGAATT 
CCAACTATCT ATTTTAAGAA ATCTCAGGAA AACAGAATTG AAAAGTTTCT AGATGTGGTG 
CAGTTAGTTC AGCGGAAGTC CTGGAAAGTG GGGGACATCT TCCAAGCCGT GGTGCAGTAC 
TGCAAGCTCA GCGAGGAGGG CAGAGAGATC ACACCCAGCC TGTTTGACTG GCTGCTTGAA 
CTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPHKAP
Entrez Gene ID	100223386
Full Name	SPHK1 interactor, AKAP domain containing
Gene Type	protein-coding
Organism	Taeniopygia guttata(zebra finch)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPHKAP cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene

Clones

gRNAs

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