Gene Symbol | SMAD4 |
Entrez Gene ID | 397142 |
Full Name | SMAD family member 4 |
Synonyms | MADH4 |
Gene Type | protein-coding |
Organism | Sus scrofa(pig) |
Learn More
Learn More
Learn More
Learn More
ORF » Species Summary » Sus scrofa » SMAD4 cDNA ORF clone
Gene Symbol | SMAD4 |
Entrez Gene ID | 397142 |
Full Name | SMAD family member 4 |
Synonyms | MADH4 |
Gene Type | protein-coding |
Organism | Sus scrofa(pig) |
mRNA | Protein | Name |
---|---|---|
XM_005654429.2 | XP_005654486.1 | mothers against decapentaplegic homolog 4 isoform X1 |
XM_005654429.3 | XP_005654486.1 | mothers against decapentaplegic homolog 4 isoform X1 |
XM_013985326.2 | XP_013840780.1 | mothers against decapentaplegic homolog 4 isoform X1 |
XM_013985326.1 | XP_013840780.1 | mothers against decapentaplegic homolog 4 isoform X1 |
XM_021082766.1 | XP_020938425.1 | mothers against decapentaplegic homolog 4 isoform X1 |
NM_214072.1 | NP_999237.1 | mothers against decapentaplegic homolog 4 |
The transcription factor SMAD4 and miR-10b contribute to E2 release and cell apoptosis in ovarian granulosa cells by targeting CYP19A1.
Li Q, Du X, Pan Z, Zhang L, Li Q
Molecular and cellular endocrinology47684-95(2018 11)
SMAD4 feedback regulates the canonical TGF-β signaling pathway to control granulosa cell apoptosis.
Du X, Pan Z, Li Q, Liu H, Li Q
Cell death & disease9(2)151(2018 02)
TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis.
Du X, Zhang L, Li X, Pan Z, Liu H, Li Q
Cell death & disease7(11)e2476(2016 11)
MicroRNA-26b functions as a proapoptotic factor in porcine follicular Granulosa cells by targeting Sma-and Mad-related protein 4.
Liu J, Du X, Zhou J, Pan Z, Liu H, Li Q
Biology of reproduction91(6)146(2014 Dec)
Interference RNA-based silencing of endogenous SMAD4 in porcine granulosa cells resulted in decreased FSH-mediated granulosa cells proliferation and steroidogenesis.
Wang W, Chen X, Li X, Wang L, Zhang H, He Y, Wang J, Zhao Y, Zhang B, Xu Y
Reproduction (Cambridge, England)141(5)643-51(2011 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The following SMAD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMAD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSe30515 | |
Clone ID Related Accession (Same CDS sequence) | NM_214072.1 | |
Accession Version | NM_214072.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1571328000000 | |
Organism | Sus scrofa(pig) | |
Product | mothers against decapentaplegic homolog 4 | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB053483.1. ##Evidence-Data-START## Transcript exon combination :: AB053483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_999237.1 |
CDS | 513..2171 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OSe02066 | |
Clone ID Related Accession (Same CDS sequence) | XM_005654429.2 , XM_005654429.3 , XM_013985326.2 , XM_013985326.1 , XM_021082766.1 | |
Accession Version | XM_005654429.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1441814400000 | |
Organism | Sus scrofa(Pig) | |
Product | mothers against decapentaplegic homolog 4 isoform X1 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533986.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005654429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005654486.1 |
CDS | 219..1877 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OSe02066 | |
Clone ID Related Accession (Same CDS sequence) | XM_005654429.2 , XM_005654429.3 , XM_013985326.2 , XM_013985326.1 , XM_021082766.1 | |
Accession Version | XM_005654429.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1494518400000 | |
Organism | Sus scrofa(pig) | |
Product | mothers against decapentaplegic homolog 4 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005654429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005654486.1 |
CDS | 403..2061 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OSe02066 | |
Clone ID Related Accession (Same CDS sequence) | XM_005654429.2 , XM_005654429.3 , XM_013985326.2 , XM_013985326.1 , XM_021082766.1 | |
Accession Version | XM_013985326.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1494518400000 | |
Organism | Sus scrofa(pig) | |
Product | mothers against decapentaplegic homolog 4 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013985326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_013840780.1 |
CDS | 263..1921 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OSe02066 | |
Clone ID Related Accession (Same CDS sequence) | XM_005654429.2 , XM_005654429.3 , XM_013985326.2 , XM_013985326.1 , XM_021082766.1 | |
Accession Version | XM_013985326.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1441814400000 | |
Organism | Sus scrofa(Pig) | |
Product | mothers against decapentaplegic homolog 4 isoform X1 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533986.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_013840780.1 |
CDS | 234..1892 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OSe02066 | |
Clone ID Related Accession (Same CDS sequence) | XM_005654429.2 , XM_005654429.3 , XM_013985326.2 , XM_013985326.1 , XM_021082766.1 | |
Accession Version | XM_021082766.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 1494518400000 | |
Organism | Sus scrofa(pig) | |
Product | mothers against decapentaplegic homolog 4 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020938425.1 |
CDS | 453..2111 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The transcription factor SMAD4 and miR-10b contribute to E2 release and cell apoptosis in ovarian granulosa cells by targeting CYP19A1. |
SMAD4 feedback regulates the canonical TGF-β signaling pathway to control granulosa cell apoptosis. |
TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis. |
MicroRNA-26b functions as a proapoptotic factor in porcine follicular Granulosa cells by targeting Sma-and Mad-related protein 4. |
Interference RNA-based silencing of endogenous SMAD4 in porcine granulosa cells resulted in decreased FSH-mediated granulosa cells proliferation and steroidogenesis. |