TSC2 gene cDNA ORF clone, Sus scrofa(pig)

mRNA	Protein	Name
XM_005655160.3	XP_005655217.1	tuberin isoform X1
XM_005655161.3	XP_005655218.1	tuberin isoform X6
XM_003354669.3	XP_003354717.1	tuberin isoform X2
XM_003354671.4	XP_003354719.1	tuberin isoform X10
XM_021086776.1	XP_020942435.1	tuberin isoform X8
XM_021086777.1	XP_020942436.1	tuberin isoform X11
XM_005655160.2	XP_005655217.1	tuberin isoform X1
XM_005655162.2	XP_005655219.1	tuberin isoform X5
XM_005655163.3	XP_005655220.1	tuberin isoform X9
XM_003354669.4	XP_003354717.1	tuberin isoform X2
XM_003354670.4	XP_003354718.1	tuberin isoform X13
XM_021086774.1	XP_020942433.1	tuberin isoform X4
XM_021086775.1	XP_020942434.1	tuberin isoform X5
XM_021086773.1	XP_020942432.1	tuberin isoform X3
XM_005655163.2	XP_005655220.1	tuberin isoform X6
XM_013987819.1	XP_013843273.1	tuberin isoform X3
XM_003354670.3	XP_003354718.1	tuberin isoform X8
XM_003354671.3	XP_003354719.1	tuberin isoform X7
XM_005655161.2	XP_005655218.1	tuberin isoform X4
XM_005655162.3	XP_005655219.1	tuberin isoform X7
XM_021086778.1	XP_020942437.1	tuberin isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe07064	XM_005655160.3 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07065	XM_003354669.4 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07066	XM_005655161.3 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07067	XM_005655162.3 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07068	XM_005655163.3 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07069	XM_003354671.4 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07070	XM_003354670.4 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245919	XM_021086773.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245920	XM_021086774.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245921	XM_021086775.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245922	XM_021086776.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245923	XM_021086777.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe245924	XM_021086778.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe33953	XM_013987819.1 Latest version!	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSe07064	XM_005655160.2	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07065	XM_003354669.3	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07068	XM_005655163.2	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07070	XM_003354670.3	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07069	XM_003354671.3	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07066	XM_005655161.2	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe07067	XM_005655162.2	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

TSC2 ( XM_005655160.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655217.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe07064
Clone ID Related Accession (Same CDS sequence)	XM_005655160.3 , XM_005655160.2
Accession Version	XM_005655160.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005655160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG 
TCCCGCTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAG GTCCAGGCGA GCCTGGTTGG CCAGGGGGGG TCCCCGAGTT GGAAGACTTG 
GAGGCGCCGC TAGACACAGG TCAGCGCTGC TGCCGCGCCG AAGCTTTCAG CAGGTCGTCC 
TCAACCTCCA GCCAGGAGGA GAAGCTGTTC CACGCAGAGG ACTTGGCAGC CGGGGGCATC 
CCCATCGAGC GGGCCGTCTC CTCGGAGGGC TCCCGGTCCT CTGTGGACCT CACCTTCCAG 
CCCTCACAGC CCCTGAGCAA GTCCAGCTCA TCGCCTGAAC TGCAGACCCT GCAGGATGTC 
CTTGGGGACC TTGGGGACAA GGCCGATGCC GGCCGACTGA GCCCTGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA TGGGGAAGGC GCTGCCTGGC CAGCTCCGGG CGAAGAGGGC 
CGGGGCCGGG GTCCTTCCCA GACCGAGGGC CCCTTACCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCTCGAGG CTACACCATC TCTGATTCGG CCCCGTCGCG CAGGGGCAAG 
CGGGTGGAGA GGGATGCCTT CAAGGGCAGA GCCAGGACCC CCAACACGGA GAAGGTGCCG 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CCCCTTTCTT CGGTGACGAG 
TCCAACAAGC CCATCCTTCT GCCCAGCGAG TCTTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCGT CATATGACAC GCACAAGATC GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGTGAGC TTGCCATCCT GTCCAACGAG CACGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGT 
GGCTTGGACG TGTGTGGCGA GGACGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGATTTC GTGTCCATCG TCTACAACGA CTCGGGCGAG 
GACTTCAAGC TGGGCACCAT CAAAGGCCAG TTCAACTTTG TCCACGTGAT CATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCGCTG CAGTGCAGGA AAGACATGGA GGGCCTCGTG 
GACACCAGTG TGGCCAAGAT TGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCATG CGAACATGGC CTCGCAGGTG CACCATAGCC GATCCAACCC CACGGACATC 
TACCCCTCCA AGTGGATCGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA ACGGATCCGA 
GAGGAGGCCC ACTACTCGAA CGCCAGCCTG CCCCAGATGC AGACGCACCC TCCCGGCCAC 
GCCAAGGCCC CGGCGCAGGC CCCAGCGGAG CCCACGCCCA CCTACGAGAC GGGCCAGCGC 
AAGCGTCTCA TCTCCTCCGT GGACGACTTC ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655217.1
CDS	135..5579
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655160.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655160.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG 
TCCCGCTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAG GTCCAGGCGA GCCTGGTTGG CCAGGGGGGG TCCCCGAGTT GGAAGACTTG 
GAGGCGCCGC TAGACACAGG TCAGCGCTGC TGCCGCGCCG AAGCTTTCAG CAGGTCGTCC 
TCAACCTCCA GCCAGGAGGA GAAGCTGTTC CACGCAGAGG ACTTGGCAGC CGGGGGCATC 
CCCATCGAGC GGGCCGTCTC CTCGGAGGGC TCCCGGTCCT CTGTGGACCT CACCTTCCAG 
CCCTCACAGC CCCTGAGCAA GTCCAGCTCA TCGCCTGAAC TGCAGACCCT GCAGGATGTC 
CTTGGGGACC TTGGGGACAA GGCCGATGCC GGCCGACTGA GCCCTGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA TGGGGAAGGC GCTGCCTGGC CAGCTCCGGG CGAAGAGGGC 
CGGGGCCGGG GTCCTTCCCA GACCGAGGGC CCCTTACCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCTCGAGG CTACACCATC TCTGATTCGG CCCCGTCGCG CAGGGGCAAG 
CGGGTGGAGA GGGATGCCTT CAAGGGCAGA GCCAGGACCC CCAACACGGA GAAGGTGCCG 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CCCCTTTCTT CGGTGACGAG 
TCCAACAAGC CCATCCTTCT GCCCAGCGAG TCTTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCGT CATATGACAC GCACAAGATC GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGTGAGC TTGCCATCCT GTCCAACGAG CACGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGT 
GGCTTGGACG TGTGTGGCGA GGACGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGATTTC GTGTCCATCG TCTACAACGA CTCGGGCGAG 
GACTTCAAGC TGGGCACCAT CAAAGGCCAG TTCAACTTTG TCCACGTGAT CATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCGCTG CAGTGCAGGA AAGACATGGA GGGCCTCGTG 
GACACCAGTG TGGCCAAGAT TGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCATG CGAACATGGC CTCGCAGGTG CACCATAGCC GATCCAACCC CACGGACATC 
TACCCCTCCA AGTGGATCGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA ACGGATCCGA 
GAGGAGGCCC ACTACTCGAA CGCCAGCCTG CCCCAGATGC AGACGCACCC TCCCGGCCAC 
GCCAAGGCCC CGGCGCAGGC CCCAGCGGAG CCCACGCCCA CCTACGAGAC GGGCCAGCGC 
AAGCGTCTCA TCTCCTCCGT GGACGACTTC ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655160.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655217.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OSe07064
Clone ID Related Accession (Same CDS sequence)	XM_005655160.3 , XM_005655160.2
Accession Version	XM_005655160.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005655160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG 
TCCCGCTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAG GTCCAGGCGA GCCTGGTTGG CCAGGGGGGG TCCCCGAGTT GGAAGACTTG 
GAGGCGCCGC TAGACACAGG TCAGCGCTGC TGCCGCGCCG AAGCTTTCAG CAGGTCGTCC 
TCAACCTCCA GCCAGGAGGA GAAGCTGTTC CACGCAGAGG ACTTGGCAGC CGGGGGCATC 
CCCATCGAGC GGGCCGTCTC CTCGGAGGGC TCCCGGTCCT CTGTGGACCT CACCTTCCAG 
CCCTCACAGC CCCTGAGCAA GTCCAGCTCA TCGCCTGAAC TGCAGACCCT GCAGGATGTC 
CTTGGGGACC TTGGGGACAA GGCCGATGCC GGCCGACTGA GCCCTGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA TGGGGAAGGC GCTGCCTGGC CAGCTCCGGG CGAAGAGGGC 
CGGGGCCGGG GTCCTTCCCA GACCGAGGGC CCCTTACCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCTCGAGG CTACACCATC TCTGATTCGG CCCCGTCGCG CAGGGGCAAG 
CGGGTGGAGA GGGATGCCTT CAAGGGCAGA GCCAGGACCC CCAACACGGA GAAGGTGCCG 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CCCCTTTCTT CGGTGACGAG 
TCCAACAAGC CCATCCTTCT GCCCAGCGAG TCTTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCGT CATATGACAC GCACAAGATC GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGTGAGC TTGCCATCCT GTCCAACGAG CACGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGT 
GGCTTGGACG TGTGTGGCGA GGACGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGATTTC GTGTCCATCG TCTACAACGA CTCGGGCGAG 
GACTTCAAGC TGGGCACCAT CAAAGGCCAG TTCAACTTTG TCCACGTGAT CATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCGCTG CAGTGCAGGA AAGACATGGA GGGCCTCGTG 
GACACCAGTG TGGCCAAGAT TGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCATG CGAACATGGC CTCGCAGGTG CACCATAGCC GATCCAACCC CACGGACATC 
TACCCCTCCA AGTGGATCGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA ACGGATCCGA 
GAGGAGGCCC ACTACTCGAA CGCCAGCCTG CCCCAGATGC AGACGCACCC TCCCGGCCAC 
GCCAAGGCCC CGGCGCAGGC CCCAGCGGAG CCCACGCCCA CCTACGAGAC GGGCCAGCGC 
AAGCGTCTCA TCTCCTCCGT GGACGACTTC ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655217.1
CDS	134..5578
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655160.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655160.2

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG 
TCCCGCTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAG GTCCAGGCGA GCCTGGTTGG CCAGGGGGGG TCCCCGAGTT GGAAGACTTG 
GAGGCGCCGC TAGACACAGG TCAGCGCTGC TGCCGCGCCG AAGCTTTCAG CAGGTCGTCC 
TCAACCTCCA GCCAGGAGGA GAAGCTGTTC CACGCAGAGG ACTTGGCAGC CGGGGGCATC 
CCCATCGAGC GGGCCGTCTC CTCGGAGGGC TCCCGGTCCT CTGTGGACCT CACCTTCCAG 
CCCTCACAGC CCCTGAGCAA GTCCAGCTCA TCGCCTGAAC TGCAGACCCT GCAGGATGTC 
CTTGGGGACC TTGGGGACAA GGCCGATGCC GGCCGACTGA GCCCTGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA TGGGGAAGGC GCTGCCTGGC CAGCTCCGGG CGAAGAGGGC 
CGGGGCCGGG GTCCTTCCCA GACCGAGGGC CCCTTACCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCTCGAGG CTACACCATC TCTGATTCGG CCCCGTCGCG CAGGGGCAAG 
CGGGTGGAGA GGGATGCCTT CAAGGGCAGA GCCAGGACCC CCAACACGGA GAAGGTGCCG 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CCCCTTTCTT CGGTGACGAG 
TCCAACAAGC CCATCCTTCT GCCCAGCGAG TCTTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCGT CATATGACAC GCACAAGATC GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGTGAGC TTGCCATCCT GTCCAACGAG CACGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGT 
GGCTTGGACG TGTGTGGCGA GGACGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGATTTC GTGTCCATCG TCTACAACGA CTCGGGCGAG 
GACTTCAAGC TGGGCACCAT CAAAGGCCAG TTCAACTTTG TCCACGTGAT CATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCGCTG CAGTGCAGGA AAGACATGGA GGGCCTCGTG 
GACACCAGTG TGGCCAAGAT TGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCATG CGAACATGGC CTCGCAGGTG CACCATAGCC GATCCAACCC CACGGACATC 
TACCCCTCCA AGTGGATCGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA ACGGATCCGA 
GAGGAGGCCC ACTACTCGAA CGCCAGCCTG CCCCAGATGC AGACGCACCC TCCCGGCCAC 
GCCAAGGCCC CGGCGCAGGC CCCAGCGGAG CCCACGCCCA CCTACGAGAC GGGCCAGCGC 
AAGCGTCTCA TCTCCTCCGT GGACGACTTC ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354669.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354717.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OSe07065
Clone ID Related Accession (Same CDS sequence)	XM_003354669.3 , XM_003354669.4
Accession Version	XM_003354669.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003354669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354717.1
CDS	134..5512
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354669.3
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354669.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354669.4 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354717.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe07065
Clone ID Related Accession (Same CDS sequence)	XM_003354669.3 , XM_003354669.4
Accession Version	XM_003354669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003354669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354717.1
CDS	135..5513
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354669.4
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354669.4

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655161.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655218.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OSe07066
Clone ID Related Accession (Same CDS sequence)	XM_005655161.3 , XM_005655161.2
Accession Version	XM_005655161.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005655161.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCCGC 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655218.1
CDS	135..5453
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655161.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655161.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCCGC 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655161.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655218.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OSe07066
Clone ID Related Accession (Same CDS sequence)	XM_005655161.3 , XM_005655161.2
Accession Version	XM_005655161.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005655161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCCGC 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655218.1
CDS	134..5452
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655161.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655161.2

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCCGC 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGTCTTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC 
CCGTCATATG ACACGCACAA GATCGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAACAGT 
GAGCTTGCCA TCCTGTCCAA CGAGCACGGC TCCTACAGGT ACACGGAGTT CCTGACGGGG 
CTGGGCAAGC TCATCGAGCT CAAGGACTGC CAGCCGGACA AGGTGTACCT GGGTGGCTTG 
GACGTGTGTG GCGAGGACGG CCAGTTCACC TACTGCTGGC ACGACGACAT CATGCAAGCC 
GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA TTTCGTGTCC ATCGTCTACA ACGACTCGGG CGAGGACTTC 
AAGCTGGGCA CCATCAAAGG CCAGTTCAAC TTTGTCCACG TGATCATCAC GCCCCTGGAC 
TACGAGTGCA ACCTGGTGTC GCTGCAGTGC AGGAAAGACA TGGAGGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGAAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG 
CATGCGAACA TGGCCTCGCA GGTGCACCAT AGCCGATCCA ACCCCACGGA CATCTACCCC 
TCCAAGTGGA TCGCCAGGCT CCGCCACATC AAGCGGCTGC GCCAACGGAT CCGAGAGGAG 
GCCCACTACT CGAACGCCAG CCTGCCCCAG ATGCAGACGC ACCCTCCCGG CCACGCCAAG 
GCCCCGGCGC AGGCCCCAGC GGAGCCCACG CCCACCTACG AGACGGGCCA GCGCAAGCGT 
CTCATCTCCT CCGTGGACGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655162.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655219.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OSe07067
Clone ID Related Accession (Same CDS sequence)	XM_005655162.2 , XM_005655162.3
Accession Version	XM_005655162.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005655162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655219.1
CDS	134..5449
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655162.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655162.2

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655162.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655219.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OSe07067
Clone ID Related Accession (Same CDS sequence)	XM_005655162.2 , XM_005655162.3
Accession Version	XM_005655162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005655162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655219.1
CDS	135..5450
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655162.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655162.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655163.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655220.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OSe07068
Clone ID Related Accession (Same CDS sequence)	XM_005655163.3 , XM_005655163.2
Accession Version	XM_005655163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005655163.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655220.1
CDS	135..5384
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655163.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655163.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005655163.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655220.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OSe07068
Clone ID Related Accession (Same CDS sequence)	XM_005655163.3 , XM_005655163.2
Accession Version	XM_005655163.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005655163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655220.1
CDS	134..5383
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005655163.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655163.2

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACA TCGCTCACGA GCGCCAGCTT GGGGTCTGCA 
GATGAGAACT CGATGGCCCA GGCTGACGAC AGCCTGAAGA ACCTGCACCT GGAGCTCACA 
GAAACCTGTC TAGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACGGC CGTGCCCAAG 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354671.4 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354719.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.

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CloneID	OSe07069
Clone ID Related Accession (Same CDS sequence)	XM_003354671.4 , XM_003354671.3
Accession Version	XM_003354671.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003354671.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGTCTTTTG AGCGGTCGGT GCAGCTCCTC 
GACCAGATCC CGTCATATGA CACGCACAAG ATCGCCGTCC TGTACGTGGG AGAAGGCCAG 
AGCAACAGTG AGCTTGCCAT CCTGTCCAAC GAGCACGGCT CCTACAGGTA CACGGAGTTC 
CTGACGGGGC TGGGCAAGCT CATCGAGCTC AAGGACTGCC AGCCGGACAA GGTGTACCTG 
GGTGGCTTGG ACGTGTGTGG CGAGGACGGC CAGTTCACCT ACTGCTGGCA CGACGACATC 
ATGCAAGCCG TCTTCCACAT CGCCACCCTG ATGCCCACCA AGGACGTGGA CAAGCACCGC 
TGTGACAAGA AGCGCCACCT GGGCAATGAT TTCGTGTCCA TCGTCTACAA CGACTCGGGC 
GAGGACTTCA AGCTGGGCAC CATCAAAGGC CAGTTCAACT TTGTCCACGT GATCATCACG 
CCCCTGGACT ACGAGTGCAA CCTGGTGTCG CTGCAGTGCA GGAAAGACAT GGAGGGCCTC 
GTGGACACCA GTGTGGCCAA GATTGTGTCT GACCGAAACC TGCCCTTCGT GGCCCGCCAG 
ATGGCCCTGC ATGCGAACAT GGCCTCGCAG GTGCACCATA GCCGATCCAA CCCCACGGAC 
ATCTACCCCT CCAAGTGGAT CGCCAGGCTC CGCCACATCA AGCGGCTGCG CCAACGGATC 
CGAGAGGAGG CCCACTACTC GAACGCCAGC CTGCCCCAGA TGCAGACGCA CCCTCCCGGC 
CACGCCAAGG CCCCGGCGCA GGCCCCAGCG GAGCCCACGC CCACCTACGA GACGGGCCAG 
CGCAAGCGTC TCATCTCCTC CGTGGACGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354719.1
CDS	155..5602
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354671.4
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354671.4

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGTCTTTTG AGCGGTCGGT GCAGCTCCTC 
GACCAGATCC CGTCATATGA CACGCACAAG ATCGCCGTCC TGTACGTGGG AGAAGGCCAG 
AGCAACAGTG AGCTTGCCAT CCTGTCCAAC GAGCACGGCT CCTACAGGTA CACGGAGTTC 
CTGACGGGGC TGGGCAAGCT CATCGAGCTC AAGGACTGCC AGCCGGACAA GGTGTACCTG 
GGTGGCTTGG ACGTGTGTGG CGAGGACGGC CAGTTCACCT ACTGCTGGCA CGACGACATC 
ATGCAAGCCG TCTTCCACAT CGCCACCCTG ATGCCCACCA AGGACGTGGA CAAGCACCGC 
TGTGACAAGA AGCGCCACCT GGGCAATGAT TTCGTGTCCA TCGTCTACAA CGACTCGGGC 
GAGGACTTCA AGCTGGGCAC CATCAAAGGC CAGTTCAACT TTGTCCACGT GATCATCACG 
CCCCTGGACT ACGAGTGCAA CCTGGTGTCG CTGCAGTGCA GGAAAGACAT GGAGGGCCTC 
GTGGACACCA GTGTGGCCAA GATTGTGTCT GACCGAAACC TGCCCTTCGT GGCCCGCCAG 
ATGGCCCTGC ATGCGAACAT GGCCTCGCAG GTGCACCATA GCCGATCCAA CCCCACGGAC 
ATCTACCCCT CCAAGTGGAT CGCCAGGCTC CGCCACATCA AGCGGCTGCG CCAACGGATC 
CGAGAGGAGG CCCACTACTC GAACGCCAGC CTGCCCCAGA TGCAGACGCA CCCTCCCGGC 
CACGCCAAGG CCCCGGCGCA GGCCCCAGCG GAGCCCACGC CCACCTACGA GACGGGCCAG 
CGCAAGCGTC TCATCTCCTC CGTGGACGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354671.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354719.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OSe07069
Clone ID Related Accession (Same CDS sequence)	XM_003354671.4 , XM_003354671.3
Accession Version	XM_003354671.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003354671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGTCTTTTG AGCGGTCGGT GCAGCTCCTC 
GACCAGATCC CGTCATATGA CACGCACAAG ATCGCCGTCC TGTACGTGGG AGAAGGCCAG 
AGCAACAGTG AGCTTGCCAT CCTGTCCAAC GAGCACGGCT CCTACAGGTA CACGGAGTTC 
CTGACGGGGC TGGGCAAGCT CATCGAGCTC AAGGACTGCC AGCCGGACAA GGTGTACCTG 
GGTGGCTTGG ACGTGTGTGG CGAGGACGGC CAGTTCACCT ACTGCTGGCA CGACGACATC 
ATGCAAGCCG TCTTCCACAT CGCCACCCTG ATGCCCACCA AGGACGTGGA CAAGCACCGC 
TGTGACAAGA AGCGCCACCT GGGCAATGAT TTCGTGTCCA TCGTCTACAA CGACTCGGGC 
GAGGACTTCA AGCTGGGCAC CATCAAAGGC CAGTTCAACT TTGTCCACGT GATCATCACG 
CCCCTGGACT ACGAGTGCAA CCTGGTGTCG CTGCAGTGCA GGAAAGACAT GGAGGGCCTC 
GTGGACACCA GTGTGGCCAA GATTGTGTCT GACCGAAACC TGCCCTTCGT GGCCCGCCAG 
ATGGCCCTGC ATGCGAACAT GGCCTCGCAG GTGCACCATA GCCGATCCAA CCCCACGGAC 
ATCTACCCCT CCAAGTGGAT CGCCAGGCTC CGCCACATCA AGCGGCTGCG CCAACGGATC 
CGAGAGGAGG CCCACTACTC GAACGCCAGC CTGCCCCAGA TGCAGACGCA CCCTCCCGGC 
CACGCCAAGG CCCCGGCGCA GGCCCCAGCG GAGCCCACGC CCACCTACGA GACGGGCCAG 
CGCAAGCGTC TCATCTCCTC CGTGGACGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354719.1
CDS	151..5598
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354671.3
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354671.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGTCTTTTG AGCGGTCGGT GCAGCTCCTC 
GACCAGATCC CGTCATATGA CACGCACAAG ATCGCCGTCC TGTACGTGGG AGAAGGCCAG 
AGCAACAGTG AGCTTGCCAT CCTGTCCAAC GAGCACGGCT CCTACAGGTA CACGGAGTTC 
CTGACGGGGC TGGGCAAGCT CATCGAGCTC AAGGACTGCC AGCCGGACAA GGTGTACCTG 
GGTGGCTTGG ACGTGTGTGG CGAGGACGGC CAGTTCACCT ACTGCTGGCA CGACGACATC 
ATGCAAGCCG TCTTCCACAT CGCCACCCTG ATGCCCACCA AGGACGTGGA CAAGCACCGC 
TGTGACAAGA AGCGCCACCT GGGCAATGAT TTCGTGTCCA TCGTCTACAA CGACTCGGGC 
GAGGACTTCA AGCTGGGCAC CATCAAAGGC CAGTTCAACT TTGTCCACGT GATCATCACG 
CCCCTGGACT ACGAGTGCAA CCTGGTGTCG CTGCAGTGCA GGAAAGACAT GGAGGGCCTC 
GTGGACACCA GTGTGGCCAA GATTGTGTCT GACCGAAACC TGCCCTTCGT GGCCCGCCAG 
ATGGCCCTGC ATGCGAACAT GGCCTCGCAG GTGCACCATA GCCGATCCAA CCCCACGGAC 
ATCTACCCCT CCAAGTGGAT CGCCAGGCTC CGCCACATCA AGCGGCTGCG CCAACGGATC 
CGAGAGGAGG CCCACTACTC GAACGCCAGC CTGCCCCAGA TGCAGACGCA CCCTCCCGGC 
CACGCCAAGG CCCCGGCGCA GGCCCCAGCG GAGCCCACGC CCACCTACGA GACGGGCCAG 
CGCAAGCGTC TCATCTCCTC CGTGGACGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354670.4 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354718.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X14, mRNA.

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CloneID	OSe07070
Clone ID Related Accession (Same CDS sequence)	XM_003354670.4 , XM_003354670.3
Accession Version	XM_003354670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003354670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGTCTTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC GTCATATGAC 
ACGCACAAGA TCGCCGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGTGA GCTTGCCATC 
CTGTCCAACG AGCACGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GTGGCTTGGA CGTGTGTGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGATT TCGTGTCCAT CGTCTACAAC GACTCGGGCG AGGACTTCAA GCTGGGCACC 
ATCAAAGGCC AGTTCAACTT TGTCCACGTG ATCATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCGC TGCAGTGCAG GAAAGACATG GAGGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA TGCGAACATG 
GCCTCGCAGG TGCACCATAG CCGATCCAAC CCCACGGACA TCTACCCCTC CAAGTGGATC 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAACGGATCC GAGAGGAGGC CCACTACTCG 
AACGCCAGCC TGCCCCAGAT GCAGACGCAC CCTCCCGGCC ACGCCAAGGC CCCGGCGCAG 
GCCCCAGCGG AGCCCACGCC CACCTACGAG ACGGGCCAGC GCAAGCGTCT CATCTCCTCC 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354718.1
CDS	155..5401
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354670.4
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354670.4

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGTCTTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC GTCATATGAC 
ACGCACAAGA TCGCCGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGTGA GCTTGCCATC 
CTGTCCAACG AGCACGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GTGGCTTGGA CGTGTGTGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGATT TCGTGTCCAT CGTCTACAAC GACTCGGGCG AGGACTTCAA GCTGGGCACC 
ATCAAAGGCC AGTTCAACTT TGTCCACGTG ATCATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCGC TGCAGTGCAG GAAAGACATG GAGGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA TGCGAACATG 
GCCTCGCAGG TGCACCATAG CCGATCCAAC CCCACGGACA TCTACCCCTC CAAGTGGATC 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAACGGATCC GAGAGGAGGC CCACTACTCG 
AACGCCAGCC TGCCCCAGAT GCAGACGCAC CCTCCCGGCC ACGCCAAGGC CCCGGCGCAG 
GCCCCAGCGG AGCCCACGCC CACCTACGAG ACGGGCCAGC GCAAGCGTCT CATCTCCTCC 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003354670.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003354718.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

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CloneID	OSe07070
Clone ID Related Accession (Same CDS sequence)	XM_003354670.4 , XM_003354670.3
Accession Version	XM_003354670.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003354670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGTCTTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC GTCATATGAC 
ACGCACAAGA TCGCCGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGTGA GCTTGCCATC 
CTGTCCAACG AGCACGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GTGGCTTGGA CGTGTGTGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGATT TCGTGTCCAT CGTCTACAAC GACTCGGGCG AGGACTTCAA GCTGGGCACC 
ATCAAAGGCC AGTTCAACTT TGTCCACGTG ATCATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCGC TGCAGTGCAG GAAAGACATG GAGGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA TGCGAACATG 
GCCTCGCAGG TGCACCATAG CCGATCCAAC CCCACGGACA TCTACCCCTC CAAGTGGATC 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAACGGATCC GAGAGGAGGC CCACTACTCG 
AACGCCAGCC TGCCCCAGAT GCAGACGCAC CCTCCCGGCC ACGCCAAGGC CCCGGCGCAG 
GCCCCAGCGG AGCCCACGCC CACCTACGAG ACGGGCCAGC GCAAGCGTCT CATCTCCTCC 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003354718.1
CDS	151..5397
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003354670.3
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003354670.3

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGTCTTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC GTCATATGAC 
ACGCACAAGA TCGCCGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGTGA GCTTGCCATC 
CTGTCCAACG AGCACGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GTGGCTTGGA CGTGTGTGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGATT TCGTGTCCAT CGTCTACAAC GACTCGGGCG AGGACTTCAA GCTGGGCACC 
ATCAAAGGCC AGTTCAACTT TGTCCACGTG ATCATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCGC TGCAGTGCAG GAAAGACATG GAGGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA TGCGAACATG 
GCCTCGCAGG TGCACCATAG CCGATCCAAC CCCACGGACA TCTACCCCTC CAAGTGGATC 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAACGGATCC GAGAGGAGGC CCACTACTCG 
AACGCCAGCC TGCCCCAGAT GCAGACGCAC CCTCCCGGCC ACGCCAAGGC CCCGGCGCAG 
GCCCCAGCGG AGCCCACGCC CACCTACGAG ACGGGCCAGC GCAAGCGTCT CATCTCCTCC 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086773.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942432.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe245919
Clone ID Related Accession (Same CDS sequence)	XM_021086773.1
Accession Version	XM_021086773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942432.1
CDS	135..5510
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086773.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086773.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGGATC CAGACATCGC TCACGAGCGC CAGCTTGGGG 
TCTGCAGATG AGAACTCGAT GGCCCAGGCT GACGACAGCC TGAAGAACCT GCACCTGGAG 
CTCACAGAAA CCTGTCTAGA CATGATGGCC AGATACGTGT TCTCCAACTT CACGGCCGTG 
CCCAAGAGGT CTCCCGTCGG GGAGTTCCTT CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTGGTCAC CGTGACGACA AGCGTGGGGA CCGGGACGCG GTCACTCCTG 
GGCTTGGACT CAGGAGAGCT GCAGGGCGGC CCAGAGTTGA GCTCCGACCC CGGTGTGCAC 
GTGAGACAGA TAAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTGC 
CGCGGGGCCC GGGACCGGGT CCGCTCCATG TCTGGGGGCC ACGGCCTTCG TGTTGGCACC 
TTGGATGCTC CGGCCTCACA CTTCCCAGGC AGCCCCTCTT CCCCGGGCTC AGAGACTGCC 
CCAGCCGGCA AGCCCGAAAA GGCCTCGGCT GGCTCCCAGC TCCCTGCGCA GAAGGAGAAA 
ACAAACCTGG CGGCCTACGT CCCCCTGTTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGA 
AGACCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGGGATAC GGCGCTGTAC AAGTCCCTGT CGGTGCCGGC GGCCGGCTCG 
GCAAAGCCTC CCCCGCCCCC GCGCTCTAAC ACAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GTCTTTTGAG CGGTCGGTGC AGCTCCTCGA CCAGATCCCG 
TCATATGACA CGCACAAGAT CGCCGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGTGAG 
CTTGCCATCC TGTCCAACGA GCACGGCTCC TACAGGTACA CGGAGTTCCT GACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG TGGCTTGGAC 
GTGTGTGGCG AGGACGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCCGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGATTT CGTGTCCATC GTCTACAACG ACTCGGGCGA GGACTTCAAG 
CTGGGCACCA TCAAAGGCCA GTTCAACTTT GTCCACGTGA TCATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCGCT GCAGTGCAGG AAAGACATGG AGGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAT 
GCGAACATGG CCTCGCAGGT GCACCATAGC CGATCCAACC CCACGGACAT CTACCCCTCC 
AAGTGGATCG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AACGGATCCG AGAGGAGGCC 
CACTACTCGA ACGCCAGCCT GCCCCAGATG CAGACGCACC CTCCCGGCCA CGCCAAGGCC 
CCGGCGCAGG CCCCAGCGGA GCCCACGCCC ACCTACGAGA CGGGCCAGCG CAAGCGTCTC 
ATCTCCTCCG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086774.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942433.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OSe245920
Clone ID Related Accession (Same CDS sequence)	XM_021086774.1
Accession Version	XM_021086774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TGTGAAGCGG GCTGTGGGCC GGGGGCTTCC TCGGGCCACG GCCCCCATCA GAGCCACGGC 
AGCAAGAGCG GCGTGGGGGG CCCCGCCAGG CGCGAGGTTG GCGGACACTC CTGTGCTTTC 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942433.1
CDS	135..5504
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRCEAGCGPGASSGHGPHQSHGSKSGVGGPARREVGGHSCAFRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086774.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086774.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TGTGAAGCGG GCTGTGGGCC GGGGGCTTCC TCGGGCCACG GCCCCCATCA GAGCCACGGC 
AGCAAGAGCG GCGTGGGGGG CCCCGCCAGG CGCGAGGTTG GCGGACACTC CTGTGCTTTC 
AGGTCTCCCG TCGGGGAGTT CCTTCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTGG TCACCGTGAC GACAAGCGTG GGGACCGGGA CGCGGTCACT CCTGGGCTTG 
GACTCAGGAG AGCTGCAGGG CGGCCCAGAG TTGAGCTCCG ACCCCGGTGT GCACGTGAGA 
CAGATAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTGCCGCGGG 
GCCCGGGACC GGGTCCGCTC CATGTCTGGG GGCCACGGCC TTCGTGTTGG CACCTTGGAT 
GCTCCGGCCT CACACTTCCC AGGCAGCCCC TCTTCCCCGG GCTCAGAGAC TGCCCCAGCC 
GGCAAGCCCG AAAAGGCCTC GGCTGGCTCC CAGCTCCCTG CGCAGAAGGA GAAAACAAAC 
CTGGCGGCCT ACGTCCCCCT GTTGACCCAG GGCTGGGCGG AGATCTTGGT CCGAAGACCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAACA TGCCCCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGGG ATACGGCGCT GTACAAGTCC CTGTCGGTGC CGGCGGCCGG CTCGGCAAAG 
CCTCCCCCGC CCCCGCGCTC TAACACAGAC TCTGCGGTGG TTCTGGAGGA GGGAGGTCCA 
GGCGAGCCTG GTTGGCCAGG GGGGGTCCCC GAGTTGGAAG ACTTGGAGGC GCCGCTAGAC 
ACAGGTCAGC GCTGCTGCCG CGCCGAAGCT TTCAGCAGGT CGTCCTCAAC CTCCAGCCAG 
GAGGAGAAGC TGTTCCACGC AGAGGACTTG GCAGCCGGGG GCATCCCCAT CGAGCGGGCC 
GTCTCCTCGG AGGGCTCCCG GTCCTCTGTG GACCTCACCT TCCAGCCCTC ACAGCCCCTG 
AGCAAGTCCA GCTCATCGCC TGAACTGCAG ACCCTGCAGG ATGTCCTTGG GGACCTTGGG 
GACAAGGCCG ATGCCGGCCG ACTGAGCCCT GAGGCCAAGG CCCGGTCGCA GTCAGGGATC 
CTGGATGGGG AAGGCGCTGC CTGGCCAGCT CCGGGCGAAG AGGGCCGGGG CCGGGGTCCT 
TCCCAGACCG AGGGCCCCTT ACCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCT 
CGAGGCTACA CCATCTCTGA TTCGGCCCCG TCGCGCAGGG GCAAGCGGGT GGAGAGGGAT 
GCCTTCAAGG GCAGAGCCAG GACCCCCAAC ACGGAGAAGG TGCCGGGCAT CAACCCCAGC 
TTCGTGTTCC TGCAGCTCTA CCACTCCCCT TTCTTCGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTGCCCA GCGAGTCTTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCGTCATAT 
GACACGCACA AGATCGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAACAG TGAGCTTGCC 
ATCCTGTCCA ACGAGCACGG CTCCTACAGG TACACGGAGT TCCTGACGGG GCTGGGCAAG 
CTCATCGAGC TCAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGTGGCTT GGACGTGTGT 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ATTTCGTGTC CATCGTCTAC AACGACTCGG GCGAGGACTT CAAGCTGGGC 
ACCATCAAAG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CGCCCCTGGA CTACGAGTGC 
AACCTGGTGT CGCTGCAGTG CAGGAAAGAC ATGGAGGGCC TCGTGGACAC CAGTGTGGCC 
AAGATTGTGT CTGACCGAAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCATGCGAAC 
ATGGCCTCGC AGGTGCACCA TAGCCGATCC AACCCCACGG ACATCTACCC CTCCAAGTGG 
ATCGCCAGGC TCCGCCACAT CAAGCGGCTG CGCCAACGGA TCCGAGAGGA GGCCCACTAC 
TCGAACGCCA GCCTGCCCCA GATGCAGACG CACCCTCCCG GCCACGCCAA GGCCCCGGCG 
CAGGCCCCAG CGGAGCCCAC GCCCACCTAC GAGACGGGCC AGCGCAAGCG TCTCATCTCC 
TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086775.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942434.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OSe245921
Clone ID Related Accession (Same CDS sequence)	XM_021086775.1
Accession Version	XM_021086775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGAGCAACA GTGAGCTTGC CATCCTGTCC 
AACGAGCACG GCTCCTACAG GTACACGGAG TTCCTGACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGTGGCT TGGACGTGTG TGGCGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GATTTCGTGT CCATCGTCTA CAACGACTCG GGCGAGGACT TCAAGCTGGG CACCATCAAA 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCGCTGCAGT GCAGGAAAGA CATGGAGGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCATGCGAA CATGGCCTCG 
CAGGTGCACC ATAGCCGATC CAACCCCACG GACATCTACC CCTCCAAGTG GATCGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAACGG ATCCGAGAGG AGGCCCACTA CTCGAACGCC 
AGCCTGCCCC AGATGCAGAC GCACCCTCCC GGCCACGCCA AGGCCCCGGC GCAGGCCCCA 
GCGGAGCCCA CGCCCACCTA CGAGACGGGC CAGCGCAAGC GTCTCATCTC CTCCGTGGAC 
GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942434.1
CDS	135..5495
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086775.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086775.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCCGCT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAGGTCCAGG CGAGCCTGGT TGGCCAGGGG GGGTCCCCGA GTTGGAAGAC 
TTGGAGGCGC CGCTAGACAC AGGTCAGCGC TGCTGCCGCG CCGAAGCTTT CAGCAGGTCG 
TCCTCAACCT CCAGCCAGGA GGAGAAGCTG TTCCACGCAG AGGACTTGGC AGCCGGGGGC 
ATCCCCATCG AGCGGGCCGT CTCCTCGGAG GGCTCCCGGT CCTCTGTGGA CCTCACCTTC 
CAGCCCTCAC AGCCCCTGAG CAAGTCCAGC TCATCGCCTG AACTGCAGAC CCTGCAGGAT 
GTCCTTGGGG ACCTTGGGGA CAAGGCCGAT GCCGGCCGAC TGAGCCCTGA GGCCAAGGCC 
CGGTCGCAGT CAGGGATCCT GGATGGGGAA GGCGCTGCCT GGCCAGCTCC GGGCGAAGAG 
GGCCGGGGCC GGGGTCCTTC CCAGACCGAG GGCCCCTTAC CTTCCAGCTG CCCCCGCTCC 
CCCAGTGGCC TGCGGCCTCG AGGCTACACC ATCTCTGATT CGGCCCCGTC GCGCAGGGGC 
AAGCGGGTGG AGAGGGATGC CTTCAAGGGC AGAGCCAGGA CCCCCAACAC GGAGAAGGTG 
CCGGGCATCA ACCCCAGCTT CGTGTTCCTG CAGCTCTACC ACTCCCCTTT CTTCGGTGAC 
GAGTCCAACA AGCCCATCCT TCTGCCCAGC GAGAGCAACA GTGAGCTTGC CATCCTGTCC 
AACGAGCACG GCTCCTACAG GTACACGGAG TTCCTGACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGTGGCT TGGACGTGTG TGGCGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GATTTCGTGT CCATCGTCTA CAACGACTCG GGCGAGGACT TCAAGCTGGG CACCATCAAA 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCGCTGCAGT GCAGGAAAGA CATGGAGGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCATGCGAA CATGGCCTCG 
CAGGTGCACC ATAGCCGATC CAACCCCACG GACATCTACC CCTCCAAGTG GATCGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAACGG ATCCGAGAGG AGGCCCACTA CTCGAACGCC 
AGCCTGCCCC AGATGCAGAC GCACCCTCCC GGCCACGCCA AGGCCCCGGC GCAGGCCCCA 
GCGGAGCCCA CGCCCACCTA CGAGACGGGC CAGCGCAAGC GTCTCATCTC CTCCGTGGAC 
GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086776.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942435.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

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CloneID	OSe245922
Clone ID Related Accession (Same CDS sequence)	XM_021086776.1
Accession Version	XM_021086776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGAGCAAC AGTGAGCTTG CCATCCTGTC CAACGAGCAC 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGTGGC TTGGACGTGT GTGGCGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA TGATTTCGTG 
TCCATCGTCT ACAACGACTC GGGCGAGGAC TTCAAGCTGG GCACCATCAA AGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCGCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCATGCGA ACATGGCCTC GCAGGTGCAC 
CATAGCCGAT CCAACCCCAC GGACATCTAC CCCTCCAAGT GGATCGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAACG GATCCGAGAG GAGGCCCACT ACTCGAACGC CAGCCTGCCC 
CAGATGCAGA CGCACCCTCC CGGCCACGCC AAGGCCCCGG CGCAGGCCCC AGCGGAGCCC 
ACGCCCACCT ACGAGACGGG CCAGCGCAAG CGTCTCATCT CCTCCGTGGA CGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942435.1
CDS	135..5426
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086776.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086776.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGGCCCCCCA AACAAGGCTT GAATAACTCC 
CCACCCGTGA AAGAATTCAA GGAGAGCCTT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTTTCAG AGCACGCGGT CCGCAGCAGG ATCCAGACAT CGCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC GATGGCCCAG GCTGACGACA GCCTGAAGAA CCTGCACCTG 
GAGCTCACAG AAACCTGTCT AGACATGATG GCCAGATACG TGTTCTCCAA CTTCACGGCC 
GTGCCCAAGA GGTCTCCCGT CGGGGAGTTC CTTCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTGGT CACCGTGACG ACAAGCGTGG GGACCGGGAC GCGGTCACTC 
CTGGGCTTGG ACTCAGGAGA GCTGCAGGGC GGCCCAGAGT TGAGCTCCGA CCCCGGTGTG 
CACGTGAGAC AGATAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TGCCGCGGGG CCCGGGACCG GGTCCGCTCC ATGTCTGGGG GCCACGGCCT TCGTGTTGGC 
ACCTTGGATG CTCCGGCCTC ACACTTCCCA GGCAGCCCCT CTTCCCCGGG CTCAGAGACT 
GCCCCAGCCG GCAAGCCCGA AAAGGCCTCG GCTGGCTCCC AGCTCCCTGC GCAGAAGGAG 
AAAACAAACC TGGCGGCCTA CGTCCCCCTG TTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGAAGACCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAACAT GCCCCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGGGA TACGGCGCTG TACAAGTCCC TGTCGGTGCC GGCGGCCGGC 
TCGGCAAAGC CTCCCCCGCC CCCGCGCTCT AACACAGACT CTGCGGTGGT TCTGGAGGAG 
GGAGGTCCAG GCGAGCCTGG TTGGCCAGGG GGGGTCCCCG AGTTGGAAGA CTTGGAGGCG 
CCGCTAGACA CAGGTCAGCG CTGCTGCCGC GCCGAAGCTT TCAGCAGGTC GTCCTCAACC 
TCCAGCCAGG AGGAGAAGCT GTTCCACGCA GAGGACTTGG CAGCCGGGGG CATCCCCATC 
GAGCGGGCCG TCTCCTCGGA GGGCTCCCGG TCCTCTGTGG ACCTCACCTT CCAGCCCTCA 
CAGCCCCTGA GCAAGTCCAG CTCATCGCCT GAACTGCAGA CCCTGCAGGA TGTCCTTGGG 
GACCTTGGGG ACAAGGCCGA TGCCGGCCGA CTGAGCCCTG AGGCCAAGGC CCGGTCGCAG 
TCAGGGATCC TGGATGGGGA AGGCGCTGCC TGGCCAGCTC CGGGCGAAGA GGGCCGGGGC 
CGGGGTCCTT CCCAGACCGA GGGCCCCTTA CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCTC GAGGCTACAC CATCTCTGAT TCGGCCCCGT CGCGCAGGGG CAAGCGGGTG 
GAGAGGGATG CCTTCAAGGG CAGAGCCAGG ACCCCCAACA CGGAGAAGGT GCCGGGCATC 
AACCCCAGCT TCGTGTTCCT GCAGCTCTAC CACTCCCCTT TCTTCGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTGCCCAG CGAGAGCAAC AGTGAGCTTG CCATCCTGTC CAACGAGCAC 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGTGGC TTGGACGTGT GTGGCGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA TGATTTCGTG 
TCCATCGTCT ACAACGACTC GGGCGAGGAC TTCAAGCTGG GCACCATCAA AGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCGCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCATGCGA ACATGGCCTC GCAGGTGCAC 
CATAGCCGAT CCAACCCCAC GGACATCTAC CCCTCCAAGT GGATCGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAACG GATCCGAGAG GAGGCCCACT ACTCGAACGC CAGCCTGCCC 
CAGATGCAGA CGCACCCTCC CGGCCACGCC AAGGCCCCGG CGCAGGCCCC AGCGGAGCCC 
ACGCCCACCT ACGAGACGGG CCAGCGCAAG CGTCTCATCT CCTCCGTGGA CGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086777.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942436.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.

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CloneID	OSe245923
Clone ID Related Accession (Same CDS sequence)	XM_021086777.1
Accession Version	XM_021086777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GAGCAACAGT GAGCTTGCCA TCCTGTCCAA CGAGCACGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGG CTGGGCAAGC TCATCGAGCT CAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGTGGCTTG GACGTGTGTG GCGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGTGACAAG AAGCGCCACC TGGGCAATGA TTTCGTGTCC 
ATCGTCTACA ACGACTCGGG CGAGGACTTC AAGCTGGGCA CCATCAAAGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC GCCCCTGGAC TACGAGTGCA ACCTGGTGTC GCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT CGTGGACACC AGTGTGGCCA AGATTGTGTC TGACCGAAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CATGCGAACA TGGCCTCGCA GGTGCACCAT 
AGCCGATCCA ACCCCACGGA CATCTACCCC TCCAAGTGGA TCGCCAGGCT CCGCCACATC 
AAGCGGCTGC GCCAACGGAT CCGAGAGGAG GCCCACTACT CGAACGCCAG CCTGCCCCAG 
ATGCAGACGC ACCCTCCCGG CCACGCCAAG GCCCCGGCGC AGGCCCCAGC GGAGCCCACG 
CCCACCTACG AGACGGGCCA GCGCAAGCGT CTCATCTCCT CCGTGGACGA CTTCACCGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942436.1
CDS	135..5363
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086777.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086777.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGTGGCC TCTTTCTCCT CCCTGTGCCA GTCCCGCTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
GGTCCAGGCG AGCCTGGTTG GCCAGGGGGG GTCCCCGAGT TGGAAGACTT GGAGGCGCCG 
CTAGACACAG GTCAGCGCTG CTGCCGCGCC GAAGCTTTCA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGCTGTT CCACGCAGAG GACTTGGCAG CCGGGGGCAT CCCCATCGAG 
CGGGCCGTCT CCTCGGAGGG CTCCCGGTCC TCTGTGGACC TCACCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCCAGCTC ATCGCCTGAA CTGCAGACCC TGCAGGATGT CCTTGGGGAC 
CTTGGGGACA AGGCCGATGC CGGCCGACTG AGCCCTGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ATGGGGAAGG CGCTGCCTGG CCAGCTCCGG GCGAAGAGGG CCGGGGCCGG 
GGTCCTTCCC AGACCGAGGG CCCCTTACCT TCCAGCTGCC CCCGCTCCCC CAGTGGCCTG 
CGGCCTCGAG GCTACACCAT CTCTGATTCG GCCCCGTCGC GCAGGGGCAA GCGGGTGGAG 
AGGGATGCCT TCAAGGGCAG AGCCAGGACC CCCAACACGG AGAAGGTGCC GGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCCCCTTTCT TCGGTGACGA GTCCAACAAG 
CCCATCCTTC TGCCCAGCGA GAGCAACAGT GAGCTTGCCA TCCTGTCCAA CGAGCACGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGG CTGGGCAAGC TCATCGAGCT CAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGTGGCTTG GACGTGTGTG GCGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGTGACAAG AAGCGCCACC TGGGCAATGA TTTCGTGTCC 
ATCGTCTACA ACGACTCGGG CGAGGACTTC AAGCTGGGCA CCATCAAAGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC GCCCCTGGAC TACGAGTGCA ACCTGGTGTC GCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT CGTGGACACC AGTGTGGCCA AGATTGTGTC TGACCGAAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CATGCGAACA TGGCCTCGCA GGTGCACCAT 
AGCCGATCCA ACCCCACGGA CATCTACCCC TCCAAGTGGA TCGCCAGGCT CCGCCACATC 
AAGCGGCTGC GCCAACGGAT CCGAGAGGAG GCCCACTACT CGAACGCCAG CCTGCCCCAG 
ATGCAGACGC ACCCTCCCGG CCACGCCAAG GCCCCGGCGC AGGCCCCAGC GGAGCCCACG 
CCCACCTACG AGACGGGCCA GCGCAAGCGT CTCATCTCCT CCGTGGACGA CTTCACCGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021086778.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942437.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.

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CloneID	OSe245924
Clone ID Related Accession (Same CDS sequence)	XM_021086778.1
Accession Version	XM_021086778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGAGCAACAG TGAGCTTGCC ATCCTGTCCA ACGAGCACGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG GCTGGGCAAG CTCATCGAGC TCAAGGACTG CCAGCCGGAC 
AAGGTGTACC TGGGTGGCTT GGACGTGTGT GGCGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCACC GCTGTGACAA GAAGCGCCAC CTGGGCAATG ATTTCGTGTC CATCGTCTAC 
AACGACTCGG GCGAGGACTT CAAGCTGGGC ACCATCAAAG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CGCCCCTGGA CTACGAGTGC AACCTGGTGT CGCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TCGTGGACAC CAGTGTGGCC AAGATTGTGT CTGACCGAAA CCTGCCCTTC 
GTGGCCCGCC AGATGGCCCT GCATGCGAAC ATGGCCTCGC AGGTGCACCA TAGCCGATCC 
AACCCCACGG ACATCTACCC CTCCAAGTGG ATCGCCAGGC TCCGCCACAT CAAGCGGCTG 
CGCCAACGGA TCCGAGAGGA GGCCCACTAC TCGAACGCCA GCCTGCCCCA GATGCAGACG 
CACCCTCCCG GCCACGCCAA GGCCCCGGCG CAGGCCCCAG CGGAGCCCAC GCCCACCTAC 
GAGACGGGCC AGCGCAAGCG TCTCATCTCC TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942437.1
CDS	135..5294
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021086778.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086778.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGG TGATTGCCCG CTCCCTCTCG 
CCACCCCCCG AGCTGGAGGA GAGGGACGTG GCGGCGTACT CGGCCTCCTT GGAGGACGTG 
AAGACAGCGG TCCTGGGGCT CCTGGTCATC CTGCAGACCA AGCTCTACGC CCTGCCCGCC 
AGCCACGCCA TGCGCGTGTA TGAGACGCTC GTCAGCCACC TCCAGCTCCA CTACAAGCAC 
AGTTACACCC TGCCCATCGC CAGCAGCATC CGGCTGCAGG CCTTCGACTT CCTGCTGATG 
CTGCGGGCCG ATTCCTTGCA CCGCCTCGGG CTGCCCAACA AGGACGGGGT TGTGAGGTTC 
AGCCCCTACT GCCTCTGTGA CTACGTGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGT 
GGTCCCCTGT CACCTCCCAC CGGGCCACCT GGCCCAGTGC CCACAGGCCC CACCGTGCGG 
CTGGGCTCGC TGCCCTACTC CCTGCTCTTC CGTGTCCTTC TGCAGTGTTT GAAGCAGGAG 
ACGGACTGGA AGGTGCTCCG GCTGGTGCTC AGCAAGCTGC CCGAGTCGCT CCGCTACAAG 
GTCCTCACCT TCACGTCGCC CTGCAGCGTG GACCAGCTGT CGGCCGCCCT CTGCTCCATG 
CTTTCAGGCC CCAAGACCCT TGAGCGGCTC CGAGGCACCC CGGAAGGGTT TTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACGGCATTAA TCTCGTACCA TAACTACTTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC GCTGGCCGTC TGCAGCGTGG AGATGCCGGA TGTCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTC ACGCACATCT CGGCCACGGC CAGCATGGCC 
GTCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGGCTGC CTCACCTCTA CCGGAACTTT 
GCGGCGGAGC AGTATGCCAG CGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTCAATCAGT ACATTGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCT TACATCACGA AGGGCCTGCG CTCCAACGTT 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTTA GAGCTCGGAG TACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACATCG CTCACGAGCG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCGA TGGCCCAGGC TGACGACAGC CTGAAGAACC TGCACCTGGA GCTCACAGAA 
ACCTGTCTAG ACATGATGGC CAGATACGTG TTCTCCAACT TCACGGCCGT GCCCAAGAGG 
TCTCCCGTCG GGGAGTTCCT TCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTGGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACGC GGTCACTCCT GGGCTTGGAC 
TCAGGAGAGC TGCAGGGCGG CCCAGAGTTG AGCTCCGACC CCGGTGTGCA CGTGAGACAG 
ATAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTG CCGCGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCTGGGGGC CACGGCCTTC GTGTTGGCAC CTTGGATGCT 
CCGGCCTCAC ACTTCCCAGG CAGCCCCTCT TCCCCGGGCT CAGAGACTGC CCCAGCCGGC 
AAGCCCGAAA AGGCCTCGGC TGGCTCCCAG CTCCCTGCGC AGAAGGAGAA AACAAACCTG 
GCGGCCTACG TCCCCCTGTT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG AAGACCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGGGATA CGGCGCTGTA CAAGTCCCTG TCGGTGCCGG CGGCCGGCTC GGCAAAGCCT 
CCCCCGCCCC CGCGCTCTAA CACAGACTCT GCGGTGGTTC TGGAGGAGGG AGGTCCAGGC 
GAGCCTGGTT GGCCAGGGGG GGTCCCCGAG TTGGAAGACT TGGAGGCGCC GCTAGACACA 
GGTCAGCGCT GCTGCCGCGC CGAAGCTTTC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGCTGT TCCACGCAGA GGACTTGGCA GCCGGGGGCA TCCCCATCGA GCGGGCCGTC 
TCCTCGGAGG GCTCCCGGTC CTCTGTGGAC CTCACCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCCAGCT CATCGCCTGA ACTGCAGACC CTGCAGGATG TCCTTGGGGA CCTTGGGGAC 
AAGGCCGATG CCGGCCGACT GAGCCCTGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GATGGGGAAG GCGCTGCCTG GCCAGCTCCG GGCGAAGAGG GCCGGGGCCG GGGTCCTTCC 
CAGACCGAGG GCCCCTTACC TTCCAGCTGC CCCCGCTCCC CCAGTGGCCT GCGGCCTCGA 
GGCTACACCA TCTCTGATTC GGCCCCGTCG CGCAGGGGCA AGCGGGTGGA GAGGGATGCC 
TTCAAGGGCA GAGCCAGGAC CCCCAACACG GAGAAGGTGC CGGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCCCCTTTC TTCGGTGACG AGTCCAACAA GCCCATCCTT 
CTGCCCAGCG AGAGCAACAG TGAGCTTGCC ATCCTGTCCA ACGAGCACGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG GCTGGGCAAG CTCATCGAGC TCAAGGACTG CCAGCCGGAC 
AAGGTGTACC TGGGTGGCTT GGACGTGTGT GGCGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCACC GCTGTGACAA GAAGCGCCAC CTGGGCAATG ATTTCGTGTC CATCGTCTAC 
AACGACTCGG GCGAGGACTT CAAGCTGGGC ACCATCAAAG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CGCCCCTGGA CTACGAGTGC AACCTGGTGT CGCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TCGTGGACAC CAGTGTGGCC AAGATTGTGT CTGACCGAAA CCTGCCCTTC 
GTGGCCCGCC AGATGGCCCT GCATGCGAAC ATGGCCTCGC AGGTGCACCA TAGCCGATCC 
AACCCCACGG ACATCTACCC CTCCAAGTGG ATCGCCAGGC TCCGCCACAT CAAGCGGCTG 
CGCCAACGGA TCCGAGAGGA GGCCCACTAC TCGAACGCCA GCCTGCCCCA GATGCAGACG 
CACCCTCCCG GCCACGCCAA GGCCCCGGCG CAGGCCCCAG CGGAGCCCAC GCCCACCTAC 
GAGACGGGCC AGCGCAAGCG TCTCATCTCC TCCGTGGACG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013987819.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013843273.1 Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OSe33953
Clone ID Related Accession (Same CDS sequence)	XM_013987819.1
Accession Version	XM_013987819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	tuberin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534457.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGA CCAAGCTCTA CGCCCTGCCC 
GCCAGCCACG CCATGCGCGT GTATGAGACG CTCGTCAGCC ACCTCCAGCT CCACTACAAG 
CACAGTTACA CCCTGCCCAT CGCCAGCAGC ATCCGGCTGC AGGCCTTCGA CTTCCTGCTG 
ATGCTGCGGG CCGATTCCTT GCACCGCCTC GGGCTGCCCA ACAAGGACGG GGTTGTGAGG 
TTCAGCCCCT ACTGCCTCTG TGACTACGTG GAGCCGGAGA GAGGCTCTGA GAAGAAGGCC 
AGTGGTCCCC TGTCACCTCC CACCGGGCCA CCTGGCCCAG TGCCCACAGG CCCCACCGTG 
CGGCTGGGCT CGCTGCCCTA CTCCCTGCTC TTCCGTGTCC TTCTGCAGTG TTTGAAGCAG 
GAGACGGACT GGAAGGTGCT CCGGCTGGTG CTCAGCAAGC TGCCCGAGTC GCTCCGCTAC 
AAGGTCCTCA CCTTCACGTC GCCCTGCAGC GTGGACCAGC TGTCGGCCGC CCTCTGCTCC 
ATGCTTTCAG GCCCCAAGAC CCTTGAGCGG CTCCGAGGCA CCCCGGAAGG GTTTTCCAGA 
ACCGACCTGC ATCTCGCTGT GGTTCCCGTG CTGACGGCAT TAATCTCGTA CCATAACTAC 
TTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT CATCTACCGC 
TGCGCCAGCC AGTGTGTGGT GGCGCTGGCC GTCTGCAGCG TGGAGATGCC GGATGTCATC 
ATCAAGGCGC TGCCTGTCCT GGTGGTGAAG CTCACGCACA TCTCGGCCAC GGCCAGCATG 
GCCGTCCCGC TCCTCGAGTT CCTGTCCACT CTGGCCAGGC TGCCTCACCT CTACCGGAAC 
TTTGCGGCGG AGCAGTATGC CAGCGTGTTC GCCATCTCCC TGCCGTACAC CAACCCCTCC 
AAGTTCAATC AGTACATTGT GTGCCTGGCC CATCACGTCA TAGCCATGTG GTTCATTAGG 
TGCCGCCTGC CCTTCCGGAA AGATTTTGTT CCTTACATCA CGAAGGGCCT GCGCTCCAAC 
GTTCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGCT TTAGAGCTCG GAGTACCAGT 
CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGGCCCC CCAAACAAGG CTTGAATAAC 
TCCCCACCCG TGAAAGAATT CAAGGAGAGC CTTGCAGCCG ATGCCTTCCG GTGCCGCAGC 
ATCAGTGTTT CAGAGCACGC GGTCCGCAGC AGGATCCAGA CATCGCTCAC GAGCGCCAGC 
TTGGGGTCTG CAGATGAGAA CTCGATGGCC CAGGCTGACG ACAGCCTGAA GAACCTGCAC 
CTGGAGCTCA CAGAAACCTG TCTAGACATG ATGGCCAGAT ACGTGTTCTC CAACTTCACG 
GCCGTGCCCA AGAGGTCTCC CGTCGGGGAG TTCCTTCTGG CTGGTGGCAG GACCAAAACC 
TGGCTGGTTG GGAACAAGCT GGTCACCGTG ACGACAAGCG TGGGGACCGG GACGCGGTCA 
CTCCTGGGCT TGGACTCAGG AGAGCTGCAG GGCGGCCCAG AGTTGAGCTC CGACCCCGGT 
GTGCACGTGA GACAGATAAA GGAGGCGCCG GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG 
GTGTGCCGCG GGGCCCGGGA CCGGGTCCGC TCCATGTCTG GGGGCCACGG CCTTCGTGTT 
GGCACCTTGG ATGCTCCGGC CTCACACTTC CCAGGCAGCC CCTCTTCCCC GGGCTCAGAG 
ACTGCCCCAG CCGGCAAGCC CGAAAAGGCC TCGGCTGGCT CCCAGCTCCC TGCGCAGAAG 
GAGAAAACAA ACCTGGCGGC CTACGTCCCC CTGTTGACCC AGGGCTGGGC GGAGATCTTG 
GTCCGAAGAC CCACAGGGAA CACCAGCTGG CTGATGAGCC TGGAGAACCC GCTCAGCCCC 
TTCTCCTCGG ACATCAACAA CATGCCCCTG CAGGAGCTGT CCAATGCCCT CATGGCCGCC 
GAGCGCTTCA AGGAGCGCCG GGATACGGCG CTGTACAAGT CCCTGTCGGT GCCGGCGGCC 
GGCTCGGCAA AGCCTCCCCC GCCCCCGCGC TCTAACACAG TGGCCTCTTT CTCCTCCCTG 
TGCCAGTCCC GCTGCCAAGG AAAGCTGCAC AGGAGCATTT CCTGGGCAGA CTCTGCGGTG 
GTTCTGGAGG AGGGAGGTCC AGGCGAGCCT GGTTGGCCAG GGGGGGTCCC CGAGTTGGAA 
GACTTGGAGG CGCCGCTAGA CACAGGTCAG CGCTGCTGCC GCGCCGAAGC TTTCAGCAGG 
TCGTCCTCAA CCTCCAGCCA GGAGGAGAAG CTGTTCCACG CAGAGGACTT GGCAGCCGGG 
GGCATCCCCA TCGAGCGGGC CGTCTCCTCG GAGGGCTCCC GGTCCTCTGT GGACCTCACC 
TTCCAGCCCT CACAGCCCCT GAGCAAGTCC AGCTCATCGC CTGAACTGCA GACCCTGCAG 
GATGTCCTTG GGGACCTTGG GGACAAGGCC GATGCCGGCC GACTGAGCCC TGAGGCCAAG 
GCCCGGTCGC AGTCAGGGAT CCTGGATGGG GAAGGCGCTG CCTGGCCAGC TCCGGGCGAA 
GAGGGCCGGG GCCGGGGTCC TTCCCAGACC GAGGGCCCCT TACCTTCCAG CTGCCCCCGC 
TCCCCCAGTG GCCTGCGGCC TCGAGGCTAC ACCATCTCTG ATTCGGCCCC GTCGCGCAGG 
GGCAAGCGGG TGGAGAGGGA TGCCTTCAAG GGCAGAGCCA GGACCCCCAA CACGGAGAAG 
GTGCCGGGCA TCAACCCCAG CTTCGTGTTC CTGCAGCTCT ACCACTCCCC TTTCTTCGGT 
GACGAGTCCA ACAAGCCCAT CCTTCTGCCC AGCGAGTCTT TTGAGCGGTC GGTGCAGCTC 
CTCGACCAGA TCCCGTCATA TGACACGCAC AAGATCGCCG TCCTGTACGT GGGAGAAGGC 
CAGAGCAACA GTGAGCTTGC CATCCTGTCC AACGAGCACG GCTCCTACAG GTACACGGAG 
TTCCTGACGG GGCTGGGCAA GCTCATCGAG CTCAAGGACT GCCAGCCGGA CAAGGTGTAC 
CTGGGTGGCT TGGACGTGTG TGGCGAGGAC GGCCAGTTCA CCTACTGCTG GCACGACGAC 
ATCATGCAAG CCGTCTTCCA CATCGCCACC CTGATGCCCA CCAAGGACGT GGACAAGCAC 
CGCTGTGACA AGAAGCGCCA CCTGGGCAAT GATTTCGTGT CCATCGTCTA CAACGACTCG 
GGCGAGGACT TCAAGCTGGG CACCATCAAA GGCCAGTTCA ACTTTGTCCA CGTGATCATC 
ACGCCCCTGG ACTACGAGTG CAACCTGGTG TCGCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTCGTGGACA CCAGTGTGGC CAAGATTGTG TCTGACCGAA ACCTGCCCTT CGTGGCCCGC 
CAGATGGCCC TGCATGCGAA CATGGCCTCG CAGGTGCACC ATAGCCGATC CAACCCCACG 
GACATCTACC CCTCCAAGTG GATCGCCAGG CTCCGCCACA TCAAGCGGCT GCGCCAACGG 
ATCCGAGAGG AGGCCCACTA CTCGAACGCC AGCCTGCCCC AGATGCAGAC GCACCCTCCC 
GGCCACGCCA AGGCCCCGGC GCAGGCCCCA GCGGAGCCCA CGCCCACCTA CGAGACGGGC 
CAGCGCAAGC GTCTCATCTC CTCCGTGGAC GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013843273.1
CDS	134..5464
Translation	MAKPTGKDSGLKEKFKILLGLGTSRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRMIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGILRALFFKAIKDYPSHEDLHERLEVFKALTDNGRHINYLEEDLAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRLMEDRAFMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDVLLNIIERLLQQLQSLDSPELTAIVHDLLTTVEELCDQNEFHGSQERYFELVERYADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNEARSAVRIKVLDVLSFVLLINKQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKTKLYALPASHAMRVYETLVSHLQLHYKHSYTLPIASSIRLQAFDFLLMLRADSLHRLGLPNKDGVVRFSPYCLCDYVEPERGSEKKASGPLSPPTGPPGPVPTGPTVRLGSLPYSLLFRVLLQCLKQETDWKVLRLVLSKLPESLRYKVLTFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESLAADAFRCRSISVSEHAVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPGVHVRQIKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGTLDAPASHFPGSPSSPGSETAPAGKPEKASAGSQLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEPGWPGGVPELEDLEAPLDTGQRCCRAEAFSRSSSTSSQEEKLFHAEDLAAGGIPIERAVSSEGSRSSVDLTFQPSQPLSKSSSSPELQTLQDVLGDLGDKADAGRLSPEAKARSQSGILDGEGAAWPAPGEEGRGRGPSQTEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKGRARTPNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPSESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSNASLPQMQTHPPGHAKAPAQAPAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013987819.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013987819.1

ORF Insert Sequence:

ATGGCCAAGC CGACTGGCAA AGATTCAGGC CTGAAGGAGA AGTTCAAGAT TCTCCTGGGA 
CTGGGCACTT CAAGGCCAAA CCCCCGGTCC GCAGAGGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGACAGA TCTGCGAGGT TGCCAGAACC AAAAAGTTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CCGTCTCAGA CTTGCTGCAG CCAGAGCGGC CGCCAGAGGC CCGGCACGCA 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGGG ACCGCTTGGG CATCCTCAGG 
GCCCTCTTCT TTAAGGCCAT CAAGGATTAC CCCTCTCACG AAGACCTCCA CGAGAGGCTG 
GAGGTTTTCA AGGCGCTCAC GGACAACGGG AGGCACATCA ACTACTTGGA AGAAGACCTG 
GCCGAGTTTG TCCTGCAGTG GATGGACGTC GGCCTGACCT CCGAGTTCCT TCTGGTGCTC 
GTGAACTTGG TGAAGTTCAA CAGCTGTTAC CTTGACGAGT ACATCGCATC CATGGTCCAC 
ATGATCTGCC TGCTCTGCGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT CTCTCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCGG AGAGCCTCCC CCTCTTCATT 
GTCACACTTT GCCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCGTGCTG GAAGCTGATG 
CGTAATCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCCTCATG 
GAGGACAGGG CCTTCATGGA AGACGCCCCG CTGCTGCGGG GAGCCGTGTT CTTTGTGGGC 
ATGGCTCTGT GGGGAGCCCA CCGGCTCTAT TCGCTCAGGA ACTCCCCGAC GTCGGTGTTG 
CCGTCCTTCT ATGAGGCCAT GACCTGTCCG AACGAGGTGG TGTCCTACGA GATCGTCCTG 
TCCATAACCC GACTCATCAA GAAGTACCGG CGGGAGCTCC AGGCCGTGAC CTGGGACGTC 
CTGCTGAACA TCATTGAACG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG TCCGGAACTC 
ACAGCCATCG TCCACGACCT GCTGACCACA GTGGAGGAGC TGTGTGATCA GAACGAGTTC 
CATGGCTCTC AGGAGAGATA CTTCGAGCTC GTTGAGAGAT ATGCGGACCA GAGGCCTGAG 
TCCTCACTCC TAAATTTAAT AACCTACAGG GCCCAGTCCA TCCATCCGGC CAAGGATGGC 
TGGATTCACA ACCTGCAGCT GCTGATGGAG CGATTCTTCA GGAATGAGGC GCGCAGCGCT 
GTGCGGATCA AGGTACTGGA CGTCCTGTCC TTCGTGTTGC TCATCAACAA ACAGTTCTAC 
GAGGAGGAGC TCATCAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGACAGA 
GACCACCAGG TCCGAAAGCT TGCCACCCAG CTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATC ATTGAGAAGA CCAAGCTCTA CGCCCTGCCC 
GCCAGCCACG CCATGCGCGT GTATGAGACG CTCGTCAGCC ACCTCCAGCT CCACTACAAG 
CACAGTTACA CCCTGCCCAT CGCCAGCAGC ATCCGGCTGC AGGCCTTCGA CTTCCTGCTG 
ATGCTGCGGG CCGATTCCTT GCACCGCCTC GGGCTGCCCA ACAAGGACGG GGTTGTGAGG 
TTCAGCCCCT ACTGCCTCTG TGACTACGTG GAGCCGGAGA GAGGCTCTGA GAAGAAGGCC 
AGTGGTCCCC TGTCACCTCC CACCGGGCCA CCTGGCCCAG TGCCCACAGG CCCCACCGTG 
CGGCTGGGCT CGCTGCCCTA CTCCCTGCTC TTCCGTGTCC TTCTGCAGTG TTTGAAGCAG 
GAGACGGACT GGAAGGTGCT CCGGCTGGTG CTCAGCAAGC TGCCCGAGTC GCTCCGCTAC 
AAGGTCCTCA CCTTCACGTC GCCCTGCAGC GTGGACCAGC TGTCGGCCGC CCTCTGCTCC 
ATGCTTTCAG GCCCCAAGAC CCTTGAGCGG CTCCGAGGCA CCCCGGAAGG GTTTTCCAGA 
ACCGACCTGC ATCTCGCTGT GGTTCCCGTG CTGACGGCAT TAATCTCGTA CCATAACTAC 
TTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT CATCTACCGC 
TGCGCCAGCC AGTGTGTGGT GGCGCTGGCC GTCTGCAGCG TGGAGATGCC GGATGTCATC 
ATCAAGGCGC TGCCTGTCCT GGTGGTGAAG CTCACGCACA TCTCGGCCAC GGCCAGCATG 
GCCGTCCCGC TCCTCGAGTT CCTGTCCACT CTGGCCAGGC TGCCTCACCT CTACCGGAAC 
TTTGCGGCGG AGCAGTATGC CAGCGTGTTC GCCATCTCCC TGCCGTACAC CAACCCCTCC 
AAGTTCAATC AGTACATTGT GTGCCTGGCC CATCACGTCA TAGCCATGTG GTTCATTAGG 
TGCCGCCTGC CCTTCCGGAA AGATTTTGTT CCTTACATCA CGAAGGGCCT GCGCTCCAAC 
GTTCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGCT TTAGAGCTCG GAGTACCAGT 
CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGGCCCC CCAAACAAGG CTTGAATAAC 
TCCCCACCCG TGAAAGAATT CAAGGAGAGC CTTGCAGCCG ATGCCTTCCG GTGCCGCAGC 
ATCAGTGTTT CAGAGCACGC GGTCCGCAGC AGGATCCAGA CATCGCTCAC GAGCGCCAGC 
TTGGGGTCTG CAGATGAGAA CTCGATGGCC CAGGCTGACG ACAGCCTGAA GAACCTGCAC 
CTGGAGCTCA CAGAAACCTG TCTAGACATG ATGGCCAGAT ACGTGTTCTC CAACTTCACG 
GCCGTGCCCA AGAGGTCTCC CGTCGGGGAG TTCCTTCTGG CTGGTGGCAG GACCAAAACC 
TGGCTGGTTG GGAACAAGCT GGTCACCGTG ACGACAAGCG TGGGGACCGG GACGCGGTCA 
CTCCTGGGCT TGGACTCAGG AGAGCTGCAG GGCGGCCCAG AGTTGAGCTC CGACCCCGGT 
GTGCACGTGA GACAGATAAA GGAGGCGCCG GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG 
GTGTGCCGCG GGGCCCGGGA CCGGGTCCGC TCCATGTCTG GGGGCCACGG CCTTCGTGTT 
GGCACCTTGG ATGCTCCGGC CTCACACTTC CCAGGCAGCC CCTCTTCCCC GGGCTCAGAG 
ACTGCCCCAG CCGGCAAGCC CGAAAAGGCC TCGGCTGGCT CCCAGCTCCC TGCGCAGAAG 
GAGAAAACAA ACCTGGCGGC CTACGTCCCC CTGTTGACCC AGGGCTGGGC GGAGATCTTG 
GTCCGAAGAC CCACAGGGAA CACCAGCTGG CTGATGAGCC TGGAGAACCC GCTCAGCCCC 
TTCTCCTCGG ACATCAACAA CATGCCCCTG CAGGAGCTGT CCAATGCCCT CATGGCCGCC 
GAGCGCTTCA AGGAGCGCCG GGATACGGCG CTGTACAAGT CCCTGTCGGT GCCGGCGGCC 
GGCTCGGCAA AGCCTCCCCC GCCCCCGCGC TCTAACACAG TGGCCTCTTT CTCCTCCCTG 
TGCCAGTCCC GCTGCCAAGG AAAGCTGCAC AGGAGCATTT CCTGGGCAGA CTCTGCGGTG 
GTTCTGGAGG AGGGAGGTCC AGGCGAGCCT GGTTGGCCAG GGGGGGTCCC CGAGTTGGAA 
GACTTGGAGG CGCCGCTAGA CACAGGTCAG CGCTGCTGCC GCGCCGAAGC TTTCAGCAGG 
TCGTCCTCAA CCTCCAGCCA GGAGGAGAAG CTGTTCCACG CAGAGGACTT GGCAGCCGGG 
GGCATCCCCA TCGAGCGGGC CGTCTCCTCG GAGGGCTCCC GGTCCTCTGT GGACCTCACC 
TTCCAGCCCT CACAGCCCCT GAGCAAGTCC AGCTCATCGC CTGAACTGCA GACCCTGCAG 
GATGTCCTTG GGGACCTTGG GGACAAGGCC GATGCCGGCC GACTGAGCCC TGAGGCCAAG 
GCCCGGTCGC AGTCAGGGAT CCTGGATGGG GAAGGCGCTG CCTGGCCAGC TCCGGGCGAA 
GAGGGCCGGG GCCGGGGTCC TTCCCAGACC GAGGGCCCCT TACCTTCCAG CTGCCCCCGC 
TCCCCCAGTG GCCTGCGGCC TCGAGGCTAC ACCATCTCTG ATTCGGCCCC GTCGCGCAGG 
GGCAAGCGGG TGGAGAGGGA TGCCTTCAAG GGCAGAGCCA GGACCCCCAA CACGGAGAAG 
GTGCCGGGCA TCAACCCCAG CTTCGTGTTC CTGCAGCTCT ACCACTCCCC TTTCTTCGGT 
GACGAGTCCA ACAAGCCCAT CCTTCTGCCC AGCGAGTCTT TTGAGCGGTC GGTGCAGCTC 
CTCGACCAGA TCCCGTCATA TGACACGCAC AAGATCGCCG TCCTGTACGT GGGAGAAGGC 
CAGAGCAACA GTGAGCTTGC CATCCTGTCC AACGAGCACG GCTCCTACAG GTACACGGAG 
TTCCTGACGG GGCTGGGCAA GCTCATCGAG CTCAAGGACT GCCAGCCGGA CAAGGTGTAC 
CTGGGTGGCT TGGACGTGTG TGGCGAGGAC GGCCAGTTCA CCTACTGCTG GCACGACGAC 
ATCATGCAAG CCGTCTTCCA CATCGCCACC CTGATGCCCA CCAAGGACGT GGACAAGCAC 
CGCTGTGACA AGAAGCGCCA CCTGGGCAAT GATTTCGTGT CCATCGTCTA CAACGACTCG 
GGCGAGGACT TCAAGCTGGG CACCATCAAA GGCCAGTTCA ACTTTGTCCA CGTGATCATC 
ACGCCCCTGG ACTACGAGTG CAACCTGGTG TCGCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTCGTGGACA CCAGTGTGGC CAAGATTGTG TCTGACCGAA ACCTGCCCTT CGTGGCCCGC 
CAGATGGCCC TGCATGCGAA CATGGCCTCG CAGGTGCACC ATAGCCGATC CAACCCCACG 
GACATCTACC CCTCCAAGTG GATCGCCAGG CTCCGCCACA TCAAGCGGCT GCGCCAACGG 
ATCCGAGAGG AGGCCCACTA CTCGAACGCC AGCCTGCCCC AGATGCAGAC GCACCCTCCC 
GGCCACGCCA AGGCCCCGGC GCAGGCCCCA GCGGAGCCCA CGCCCACCTA CGAGACGGGC 
CAGCGCAAGC GTCTCATCTC CTCCGTGGAC GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	100505408
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 3 Map Location: 3\|3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

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