BLM cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	BLM
Entrez Gene ID	100141304
Full Name	Bloom syndrome RecQ like helicase
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 7 Map Location: 7\|7

mRNA and Protein(s)

mRNA	Protein	Name
XM_021097882.1	XP_020953541.1	Bloom syndrome protein isoform X2
NM_001123084.2	NP_001116556.1	Bloom syndrome protein
XM_021097881.1	XP_020953540.1	Bloom syndrome protein isoform X1
XM_021097885.1	XP_020953544.1	Bloom syndrome protein isoform X4
NM_001123084.1	NP_001116556.1	Bloom syndrome protein
XM_021097883.1	XP_020953542.1	Bloom syndrome protein isoform X3
XM_021097884.1	XP_020953543.1	Bloom syndrome protein isoform X4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Isolation and sequencing of pig Blm and Ubl-1/SUMO-1 genes that relate to the recombination machinery.
Matsunami K, Otsuka H, Xu H, Furidaus S, Ishimaru A, Fukuzawa M, Miyagawa S
Transplantation proceedings40(8)2773-5(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe28610	NM_001123084.2 Latest version!	Sus scrofa BLM RecQ like helicase (BLM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe222269	XM_021097881.1 Latest version!	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe222270	XM_021097882.1 Latest version!	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe222271	XM_021097883.1 Latest version!	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe222272	XM_021097885.1 Latest version!	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe222272	XM_021097884.1 Latest version!	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSe28610	NM_001123084.1	Sus scrofa Bloom syndrome RecQ like helicase (BLM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLM ( NM_001123084.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001116556.1 Sus scrofa BLM RecQ like helicase (BLM), mRNA.

Price & Availability↑

CloneID	OSe28610
Clone ID Related Accession (Same CDS sequence)	NM_001123084.2 , NM_001123084.1
Accession Version	NM_001123084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1533830400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB355635.1. On May 16, 2017 this sequence version replaced NM_001123084.1. ##Evidence-Data-START## Transcript exon combination :: AB355635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103886114, SAMEA103886115 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
GACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAGTT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTGACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCGGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116556.1
CDS	7..4287
Translation	MAAVPQNNLQEQLERHSARKLNNKLSLSKPKSSGFTFKKKAPSAKDLSITSVSVAKTPVLSDKDVNVTEAFSFSEPLSHTTDQHKRINDFKNTPAGQQTKRVSSQQPLLPDLSQVPQEVLCTSQNTPVIKESRDATFKKLEFSSSSDSCITFNDWDDMDDFDTSGNSKTFVTPLRNNFVRVSTAQKSKKSKRNFLKSQFDKINTVKTDLIPPSSESKQAYLTKKQKDDSEWLSSDVICIDDDDPNSEEHINEDTQENRSLKTHLEEKRDNCEKEKSLEETELHSVEKSSCIELNEDDYDIDYVPPSPEEGMTSTSSSSLKCSSMLKDLDTSDRRDGLGTSEDLSEPEKMTSQQPNPETSTDCEAGQINVQQQLIRVMDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGSSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFGGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001123084.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa BLM RecQ like helicase (BLM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123084.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
GACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAGTT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTGACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCGGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001123084.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001116556.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), mRNA.

Price & Availability↑

CloneID	OSe28610
Clone ID Related Accession (Same CDS sequence)	NM_001123084.2 , NM_001123084.1
Accession Version	NM_001123084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1477584000000
Organism	Sus scrofa(Pig)
Product	Bloom syndrome protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB355635.1. ##Evidence-Data-START## Transcript exon combination :: AB355635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01048372, SAMN01915391 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCTG TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
GACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAGTT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTGACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCGGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116556.1
CDS	8..4288
Translation	MAAVPQNNLQEQLERHSARKLNNKLSLSKPKSSGFTFKKKAPSAKDLSITSVSVAKTPVLSDKDVNVTEAFSFSEPLSHTTDQHKRINDFKNTPAGQQTKRVSSQQPLLPDLSQVPQEVLCTSQNTPVIKESRDATFKKLEFSSSSDSCITFNDWDDMDDFDTSGNSKTFVTPLRNNFVRVSTAQKSKKSKRNFLKSQFDKINTVKTDLIPPSSESKQAYLTKKQKDDSEWLSSDVICIDDDDPNSEEHINEDTQENRSLKTHLEEKRDNCEKEKSLEETELHSVEKSSCIELNEDDYDIDYVPPSPEEGMTSTSSSSLKCSSMLKDLDTSDRRDGLGTSEDLSEPEKMTSQQPNPETSTDCEAGQINVQQQLIRVMDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGSSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFGGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001123084.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123084.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
GACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAGTT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTGACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCGGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021097881.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020953540.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

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CloneID	OSe222269
Clone ID Related Accession (Same CDS sequence)	XM_021097881.1
Accession Version	XM_021097881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA CTGGGATCAT GGCTGCTATT CCTCAGAATA ATCTACAGGA GCAACTGGAA 
CGTCATTCAG CCAGAAAACT TAATAATAAA TTAAGTCTTT CAAAACCAAA ATCTTCAGGT 
TTCACTTTTA AAAAGAAAGC ACCTTCAGCC AAAGACCTAT CTATAACTAG TGTGTCAGTA 
GCAAAAACAC CTGTGTTGAG TGACAAAGAT GTTAATGTTA CTGAGGCCTT TTCCTTCAGT 
GAACCTTTAT CCCACACCAC AGATCAGCAC AAAAGGATCA ATGACTTTAA AAATACTCCA 
GCCGGACAGC AAACTAAGAG AGTTAGTTCA CAACAACCGT TATTACCAGA TTTGTCCCAG 
GTTCCACAGG AAGTTTTGTG TACCAGCCAG AACACACCAG TTATAAAGGA ATCCCGTGAT 
GCTACTTTCA AGAAATTAGA ATTTAGTTCT TCATCAGATT CTTGTATTAC CTTCAATGAT 
TGGGATGATA TGGATGACTT TGATACTTCT GGAAATTCAA AAACATTTGT TACACCACTC 
AGAAACAACT TCGTAAGAGT AAGCACTGCT CAGAAATCAA AAAAATCTAA GAGGAACTTT 
TTAAAATCAC AGTTTGATAA AATAAATACA GTAAAGACTG ATTTGATTCC TCCATCCTCT 
GAGAGCAAGC AGGCATATTT GACTAAGAAA CAGAAGGATG ATTCAGAATG GTTAAGTAGT 
GATGTGATTT GCATCGATGA TGACGATCCC AATTCCGAAG AGCATATAAA TGAAGATACT 
CAGGAAAATC GCTCCTTAAA AACTCATTTG GAAGAGAAAA GAGATAATTG TGAAAAGGAG 
AAAAGCTTGG AAGAAACAGA ATTACATTCA GTTGAGAAAT CTTCATGTAT TGAGCTCAAT 
GAAGATGATT ATGACATAGA TTATGTTCCA CCTTCTCCAG AAGAAGGAAT GACTTCTACC 
TCTTCATCCT CTTTAAAATG CTCTAGTATG TTGAAAGATC TTGACACCTC CGACAGAAGG 
GATGGTCTTG GCACATCAGA GGACTTGTCA GAACCTGAGA AAATGACTTC ACAGCAACCT 
AATCCAGAAA CCAGCACAAA CTGTGAAGCT GGACAGATAA ATGTACAGCA GCAGCTTATC 
CGTGTGATGG ACCATATCTG TAAATTACTT GATACCATTC CTGATGACAA GCTGAAGGCT 
TTGGATTGTG GGAATGAACT GCTGCAGCAG CGGGACATGA GAAGGAAACT CCTTGCTGAA 
GCAGATTTTA ACACATGTGA TGCCAGTGCC CTTGCCTTGC TGTGGAGATG CAGGCCTGCT 
TCACTTGGTA GCCCTGTGGC ATCTGTTTCC TCACCTTCTG CATTTCCAAA TGGCAATTCC 
TGTCCTACAG GGAATTCTGT TAAGGAGTTA GCTTTTCCAC ACTGTCTCTC AAATTCCTTC 
TCTTCTGGGG AGCATTTGCT GACTACTACC CCAGGAAAGA CAGGATTCTC AGCCACCACG 
AAGACTGTCT TTGAAAGACC TCCATTCAAT TCCCATTTAC AGAAGTCTTT TGTAAGTAGC 
AACTGGGCTG AAACCCCAAG GGTAGAAAAA AGAAATGAAA GCTCTTATTT CCCAGGAAAT 
GTTCTTACAA GCACTGCTGT GAAAGATCAG AATAAACACA CTACCTCAAT AAATGACTTG 
GAAAGAGAAA TCCAGGCATC CTGTGATATT GATAATTTTG ACATAGATGA CTTTGATGAT 
GATGACGATG ACTGGGAAAA TATGATGCAT AATTTAGCAG CCAGCAAATC TTCCACAGCT 
GTCTATCAAC CTATTAAGGA AGGTCGGCCA GTCAAATCAG TATCAGAAAG GATTTCTTCA 
GCCAAGACAG ACTCTCTTCC AGTGGCATCT ACTGCTCAAA ATAAAAACTT CTCAGAGTCA 
ATTCACAATT ACACTGACAA GTCGGAGCAA AATTTAACAT CCAGAAATCT GAAACACAAG 
CATTTCCAAA GTCTTAATTT TCCTCATACA AAAGAAATGA TGAAGATTTT TCATAAAAAA 
TTTGGCCTGC ATAATTTTAG AACTAATCAG CTTGAGGCGA TCAATGCTGC ACTGCTTGGT 
GAAGACTGTT TTATCTTAAT GCCCACTGGA GGTGGTAAAA GTTTGTGTTA CCAGCTCCCT 
GCCTGTGTTT CTTCTGGGGT CACCATTGTC ATTTCCCCCT TGAGATCACT TATAGTAGAT 
CAAGTCCAAA AGCTAACTTC CTTGGATATT CCAGCTACAT ATCTGACAGG TGATAAGACT 
GACTCCGAAG CTACAAGTAT TTACCTCCAG TTATCAAAAA AAGACCCAAT TATAAAACTT 
CTATATGTCA CTCCAGAGAA GGTCTGTGCA AGTAACAGAC TGATTTCTAC TCTGGAGAAT 
CTGTACAACA GGAAGCTCTT GGCACGTTTT GTTATTGATG AAGCACATTG TGTCAGTCAG 
TGGGGACATG ATTTTCGTCC AGACTACAAA AGAATGAATA TGCTGCGCCA GAAGTTTCCC 
TCTGTTCCAG TGATGGCGCT TACAGCCACT GCCAATCCCA GGGTGCAGAA GGACATCCTC 
ACTCAGCTGA AGATTCTCAG ACCTCAGGTG TTTACCATGA GCTTTAACAG ACATAATCTA 
AAATACTATG TGTTACCCAA AAAACCTAAA AAGGTGGCTT TTGATTGCTT AGAATGGATC 
AGAAAGCATC ACCCACATGA CTCAGGGATA ATTTACTGCC TCTCCAGGCG AGAATGTGAC 
ACAATGGCTG ACACATTACA GAAAGATGGT CTCGCTGCTC TGGCTTATCA TGCTGGTCTC 
AGTGACTCTG CCAGAGATGA AGTACAGCAC AAGTGGATTA ATCAGGATGG CTGCCAGGTT 
ATCTGTGCAA CAATTGCCTT TGGAATGGGG ATTGACAAGC CTGATGTGCG ATTTGTGATC 
CACGCATCCC TCCCTAAATC TGTGGAGGGT TACTACCAAG AATCTGGCAG AGCTGGAAGA 
GATGGGGAAA TCTCGCAGTG CCTGCTCTTT TACACCTACC ACGACGTGAC CAGACTGAAG 
AGGCTCATAC TGATGGAAAA GGATGGAAAC CATCATACGA AAGAGACTCA CTTCAATAAT 
TTGTATAGCA TGGTACATTA CTGTGAAAAT ATAGCAGAAT GCAGGAGAAT ACAGCTTTTG 
GCTTACTTTG GTGAAAATGA ATTTAATCCT AATTTTTGTA AGAAGTACCC AGATGTTTCT 
TGTGATAATT GCTGTAAAAC AAAGGATTAT AAGACAAGAG ATGTGACTGA TGATGTAAAA 
AATATTGTAA GATTTGTTCA AGAACATAGT TCATCACGAG GAGCAAGAAA TATAGGTTCT 
TCTGGGAGAT TTACTATGAA TATGCTGGTC GACATTTTCC TGGGGAGTAA AAGTGCAAAA 
CTTCAGTCAG GGATATTTGG AAAGGGATCT GCTTATTCAC GACACAATGC TGAAAGACTT 
TTTAAAAAGC TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAT 
CAACCAATTG CATATATAAT GCTGGGAGAT AAAGCCCAGG CTGTACTGAA TGGTCATTTA 
AAGGTAGACT TTATGGAAAC GGAAAATTCC AGCAGTGTGA AAAAACAAAA GGCTTTAGTG 
GCAAAGGTAT CTCAGAGGGA AGAGATGGTT AAGAAATGTC TTGGAGAACT TACAGAAGTC 
TGCAAATCTC TGGGGAAAGT TTTTGGTGTC CATTACTTTA ACATCTTCAA TACCACCACT 
CTCAAGAAGC TTGCAGAATC TTTATCTTCT GATCCTGAGG TTTTGCTTCA AATTGATGGT 
GTTACAGAAG ACAAACTGGA AAAATACGGT GCAGAAGTGA TTCCAATATT ACAGAAATAC 
TCCGAATGGA CAGTGCCAGC TGAGGACAAT TCCCCACGGA GAAGCCCCTC CAGCTGCAGA 
GGCAGTGGAA GAAACGCCCC CGAGGAGTTG GATGAGGAAA CACTCATATC TTCTCACTAC 
TTTGCAAATA AAACCAAAAA TGAAAGAAAA AGGAAAAGGA TACCAGCCTC CCAAAGGTCT 
AAGAGGAGAA AAACTAGTTT CAGTGGTTCC AAGGCAAAAG GGGGGTCCGG CACAAGCAGA 
AGGATATCTT CTAAAACTAA ACCTTCCAAC GTATTTGGAT CCCGTTCAGC TTTGCTTGGT 
TCTCAAGCAG CATCAGGAGC CAGTAGAAAA TTGGGGATTA TGGCTCCACC AAAGCCTATA 
AATAGACCGT TTCTTAAGCC TTCGTATGCA TTTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020953540.1
CDS	248..4546
Translation	MRETGIMAAIPQNNLQEQLERHSARKLNNKLSLSKPKSSGFTFKKKAPSAKDLSITSVSVAKTPVLSDKDVNVTEAFSFSEPLSHTTDQHKRINDFKNTPAGQQTKRVSSQQPLLPDLSQVPQEVLCTSQNTPVIKESRDATFKKLEFSSSSDSCITFNDWDDMDDFDTSGNSKTFVTPLRNNFVRVSTAQKSKKSKRNFLKSQFDKINTVKTDLIPPSSESKQAYLTKKQKDDSEWLSSDVICIDDDDPNSEEHINEDTQENRSLKTHLEEKRDNCEKEKSLEETELHSVEKSSCIELNEDDYDIDYVPPSPEEGMTSTSSSSLKCSSMLKDLDTSDRRDGLGTSEDLSEPEKMTSQQPNPETSTNCEAGQINVQQQLIRVMDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGNSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFSGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_021097881.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021097881.1

ORF Insert Sequence:

ATGAGGGAGA CTGGGATCAT GGCTGCTATT CCTCAGAATA ATCTACAGGA GCAACTGGAA 
CGTCATTCAG CCAGAAAACT TAATAATAAA TTAAGTCTTT CAAAACCAAA ATCTTCAGGT 
TTCACTTTTA AAAAGAAAGC ACCTTCAGCC AAAGACCTAT CTATAACTAG TGTGTCAGTA 
GCAAAAACAC CTGTGTTGAG TGACAAAGAT GTTAATGTTA CTGAGGCCTT TTCCTTCAGT 
GAACCTTTAT CCCACACCAC AGATCAGCAC AAAAGGATCA ATGACTTTAA AAATACTCCA 
GCCGGACAGC AAACTAAGAG AGTTAGTTCA CAACAACCGT TATTACCAGA TTTGTCCCAG 
GTTCCACAGG AAGTTTTGTG TACCAGCCAG AACACACCAG TTATAAAGGA ATCCCGTGAT 
GCTACTTTCA AGAAATTAGA ATTTAGTTCT TCATCAGATT CTTGTATTAC CTTCAATGAT 
TGGGATGATA TGGATGACTT TGATACTTCT GGAAATTCAA AAACATTTGT TACACCACTC 
AGAAACAACT TCGTAAGAGT AAGCACTGCT CAGAAATCAA AAAAATCTAA GAGGAACTTT 
TTAAAATCAC AGTTTGATAA AATAAATACA GTAAAGACTG ATTTGATTCC TCCATCCTCT 
GAGAGCAAGC AGGCATATTT GACTAAGAAA CAGAAGGATG ATTCAGAATG GTTAAGTAGT 
GATGTGATTT GCATCGATGA TGACGATCCC AATTCCGAAG AGCATATAAA TGAAGATACT 
CAGGAAAATC GCTCCTTAAA AACTCATTTG GAAGAGAAAA GAGATAATTG TGAAAAGGAG 
AAAAGCTTGG AAGAAACAGA ATTACATTCA GTTGAGAAAT CTTCATGTAT TGAGCTCAAT 
GAAGATGATT ATGACATAGA TTATGTTCCA CCTTCTCCAG AAGAAGGAAT GACTTCTACC 
TCTTCATCCT CTTTAAAATG CTCTAGTATG TTGAAAGATC TTGACACCTC CGACAGAAGG 
GATGGTCTTG GCACATCAGA GGACTTGTCA GAACCTGAGA AAATGACTTC ACAGCAACCT 
AATCCAGAAA CCAGCACAAA CTGTGAAGCT GGACAGATAA ATGTACAGCA GCAGCTTATC 
CGTGTGATGG ACCATATCTG TAAATTACTT GATACCATTC CTGATGACAA GCTGAAGGCT 
TTGGATTGTG GGAATGAACT GCTGCAGCAG CGGGACATGA GAAGGAAACT CCTTGCTGAA 
GCAGATTTTA ACACATGTGA TGCCAGTGCC CTTGCCTTGC TGTGGAGATG CAGGCCTGCT 
TCACTTGGTA GCCCTGTGGC ATCTGTTTCC TCACCTTCTG CATTTCCAAA TGGCAATTCC 
TGTCCTACAG GGAATTCTGT TAAGGAGTTA GCTTTTCCAC ACTGTCTCTC AAATTCCTTC 
TCTTCTGGGG AGCATTTGCT GACTACTACC CCAGGAAAGA CAGGATTCTC AGCCACCACG 
AAGACTGTCT TTGAAAGACC TCCATTCAAT TCCCATTTAC AGAAGTCTTT TGTAAGTAGC 
AACTGGGCTG AAACCCCAAG GGTAGAAAAA AGAAATGAAA GCTCTTATTT CCCAGGAAAT 
GTTCTTACAA GCACTGCTGT GAAAGATCAG AATAAACACA CTACCTCAAT AAATGACTTG 
GAAAGAGAAA TCCAGGCATC CTGTGATATT GATAATTTTG ACATAGATGA CTTTGATGAT 
GATGACGATG ACTGGGAAAA TATGATGCAT AATTTAGCAG CCAGCAAATC TTCCACAGCT 
GTCTATCAAC CTATTAAGGA AGGTCGGCCA GTCAAATCAG TATCAGAAAG GATTTCTTCA 
GCCAAGACAG ACTCTCTTCC AGTGGCATCT ACTGCTCAAA ATAAAAACTT CTCAGAGTCA 
ATTCACAATT ACACTGACAA GTCGGAGCAA AATTTAACAT CCAGAAATCT GAAACACAAG 
CATTTCCAAA GTCTTAATTT TCCTCATACA AAAGAAATGA TGAAGATTTT TCATAAAAAA 
TTTGGCCTGC ATAATTTTAG AACTAATCAG CTTGAGGCGA TCAATGCTGC ACTGCTTGGT 
GAAGACTGTT TTATCTTAAT GCCCACTGGA GGTGGTAAAA GTTTGTGTTA CCAGCTCCCT 
GCCTGTGTTT CTTCTGGGGT CACCATTGTC ATTTCCCCCT TGAGATCACT TATAGTAGAT 
CAAGTCCAAA AGCTAACTTC CTTGGATATT CCAGCTACAT ATCTGACAGG TGATAAGACT 
GACTCCGAAG CTACAAGTAT TTACCTCCAG TTATCAAAAA AAGACCCAAT TATAAAACTT 
CTATATGTCA CTCCAGAGAA GGTCTGTGCA AGTAACAGAC TGATTTCTAC TCTGGAGAAT 
CTGTACAACA GGAAGCTCTT GGCACGTTTT GTTATTGATG AAGCACATTG TGTCAGTCAG 
TGGGGACATG ATTTTCGTCC AGACTACAAA AGAATGAATA TGCTGCGCCA GAAGTTTCCC 
TCTGTTCCAG TGATGGCGCT TACAGCCACT GCCAATCCCA GGGTGCAGAA GGACATCCTC 
ACTCAGCTGA AGATTCTCAG ACCTCAGGTG TTTACCATGA GCTTTAACAG ACATAATCTA 
AAATACTATG TGTTACCCAA AAAACCTAAA AAGGTGGCTT TTGATTGCTT AGAATGGATC 
AGAAAGCATC ACCCACATGA CTCAGGGATA ATTTACTGCC TCTCCAGGCG AGAATGTGAC 
ACAATGGCTG ACACATTACA GAAAGATGGT CTCGCTGCTC TGGCTTATCA TGCTGGTCTC 
AGTGACTCTG CCAGAGATGA AGTACAGCAC AAGTGGATTA ATCAGGATGG CTGCCAGGTT 
ATCTGTGCAA CAATTGCCTT TGGAATGGGG ATTGACAAGC CTGATGTGCG ATTTGTGATC 
CACGCATCCC TCCCTAAATC TGTGGAGGGT TACTACCAAG AATCTGGCAG AGCTGGAAGA 
GATGGGGAAA TCTCGCAGTG CCTGCTCTTT TACACCTACC ACGACGTGAC CAGACTGAAG 
AGGCTCATAC TGATGGAAAA GGATGGAAAC CATCATACGA AAGAGACTCA CTTCAATAAT 
TTGTATAGCA TGGTACATTA CTGTGAAAAT ATAGCAGAAT GCAGGAGAAT ACAGCTTTTG 
GCTTACTTTG GTGAAAATGA ATTTAATCCT AATTTTTGTA AGAAGTACCC AGATGTTTCT 
TGTGATAATT GCTGTAAAAC AAAGGATTAT AAGACAAGAG ATGTGACTGA TGATGTAAAA 
AATATTGTAA GATTTGTTCA AGAACATAGT TCATCACGAG GAGCAAGAAA TATAGGTTCT 
TCTGGGAGAT TTACTATGAA TATGCTGGTC GACATTTTCC TGGGGAGTAA AAGTGCAAAA 
CTTCAGTCAG GGATATTTGG AAAGGGATCT GCTTATTCAC GACACAATGC TGAAAGACTT 
TTTAAAAAGC TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAT 
CAACCAATTG CATATATAAT GCTGGGAGAT AAAGCCCAGG CTGTACTGAA TGGTCATTTA 
AAGGTAGACT TTATGGAAAC GGAAAATTCC AGCAGTGTGA AAAAACAAAA GGCTTTAGTG 
GCAAAGGTAT CTCAGAGGGA AGAGATGGTT AAGAAATGTC TTGGAGAACT TACAGAAGTC 
TGCAAATCTC TGGGGAAAGT TTTTGGTGTC CATTACTTTA ACATCTTCAA TACCACCACT 
CTCAAGAAGC TTGCAGAATC TTTATCTTCT GATCCTGAGG TTTTGCTTCA AATTGATGGT 
GTTACAGAAG ACAAACTGGA AAAATACGGT GCAGAAGTGA TTCCAATATT ACAGAAATAC 
TCCGAATGGA CAGTGCCAGC TGAGGACAAT TCCCCACGGA GAAGCCCCTC CAGCTGCAGA 
GGCAGTGGAA GAAACGCCCC CGAGGAGTTG GATGAGGAAA CACTCATATC TTCTCACTAC 
TTTGCAAATA AAACCAAAAA TGAAAGAAAA AGGAAAAGGA TACCAGCCTC CCAAAGGTCT 
AAGAGGAGAA AAACTAGTTT CAGTGGTTCC AAGGCAAAAG GGGGGTCCGG CACAAGCAGA 
AGGATATCTT CTAAAACTAA ACCTTCCAAC GTATTTGGAT CCCGTTCAGC TTTGCTTGGT 
TCTCAAGCAG CATCAGGAGC CAGTAGAAAA TTGGGGATTA TGGCTCCACC AAAGCCTATA 
AATAGACCGT TTCTTAAGCC TTCGTATGCA TTTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021097882.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020953541.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA.

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CloneID	OSe222270
Clone ID Related Accession (Same CDS sequence)	XM_021097882.1
Accession Version	XM_021097882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTA TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
AACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAATT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTAACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCAGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020953541.1
CDS	121..4401
Translation	MAAIPQNNLQEQLERHSARKLNNKLSLSKPKSSGFTFKKKAPSAKDLSITSVSVAKTPVLSDKDVNVTEAFSFSEPLSHTTDQHKRINDFKNTPAGQQTKRVSSQQPLLPDLSQVPQEVLCTSQNTPVIKESRDATFKKLEFSSSSDSCITFNDWDDMDDFDTSGNSKTFVTPLRNNFVRVSTAQKSKKSKRNFLKSQFDKINTVKTDLIPPSSESKQAYLTKKQKDDSEWLSSDVICIDDDDPNSEEHINEDTQENRSLKTHLEEKRDNCEKEKSLEETELHSVEKSSCIELNEDDYDIDYVPPSPEEGMTSTSSSSLKCSSMLKDLDTSDRRDGLGTSEDLSEPEKMTSQQPNPETSTNCEAGQINVQQQLIRVMDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGNSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFSGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_021097882.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021097882.1

ORF Insert Sequence:

ATGGCTGCTA TTCCTCAGAA TAATCTACAG GAGCAACTGG AACGTCATTC AGCCAGAAAA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATCTTCAG GTTTCACTTT TAAAAAGAAA 
GCACCTTCAG CCAAAGACCT ATCTATAACT AGTGTGTCAG TAGCAAAAAC ACCTGTGTTG 
AGTGACAAAG ATGTTAATGT TACTGAGGCC TTTTCCTTCA GTGAACCTTT ATCCCACACC 
ACAGATCAGC ACAAAAGGAT CAATGACTTT AAAAATACTC CAGCCGGACA GCAAACTAAG 
AGAGTTAGTT CACAACAACC GTTATTACCA GATTTGTCCC AGGTTCCACA GGAAGTTTTG 
TGTACCAGCC AGAACACACC AGTTATAAAG GAATCCCGTG ATGCTACTTT CAAGAAATTA 
GAATTTAGTT CTTCATCAGA TTCTTGTATT ACCTTCAATG ATTGGGATGA TATGGATGAC 
TTTGATACTT CTGGAAATTC AAAAACATTT GTTACACCAC TCAGAAACAA CTTCGTAAGA 
GTAAGCACTG CTCAGAAATC AAAAAAATCT AAGAGGAACT TTTTAAAATC ACAGTTTGAT 
AAAATAAATA CAGTAAAGAC TGATTTGATT CCTCCATCCT CTGAGAGCAA GCAGGCATAT 
TTGACTAAGA AACAGAAGGA TGATTCAGAA TGGTTAAGTA GTGATGTGAT TTGCATCGAT 
GATGACGATC CCAATTCCGA AGAGCATATA AATGAAGATA CTCAGGAAAA TCGCTCCTTA 
AAAACTCATT TGGAAGAGAA AAGAGATAAT TGTGAAAAGG AGAAAAGCTT GGAAGAAACA 
GAATTACATT CAGTTGAGAA ATCTTCATGT ATTGAGCTCA ATGAAGATGA TTATGACATA 
GATTATGTTC CACCTTCTCC AGAAGAAGGA ATGACTTCTA CCTCTTCATC CTCTTTAAAA 
TGCTCTAGTA TGTTGAAAGA TCTTGACACC TCCGACAGAA GGGATGGTCT TGGCACATCA 
GAGGACTTGT CAGAACCTGA GAAAATGACT TCACAGCAAC CTAATCCAGA AACCAGCACA 
AACTGTGAAG CTGGACAGAT AAATGTACAG CAGCAGCTTA TCCGTGTGAT GGACCATATC 
TGTAAATTAC TTGATACCAT TCCTGATGAC AAGCTGAAGG CTTTGGATTG TGGGAATGAA 
CTGCTGCAGC AGCGGGACAT GAGAAGGAAA CTCCTTGCTG AAGCAGATTT TAACACATGT 
GATGCCAGTG CCCTTGCCTT GCTGTGGAGA TGCAGGCCTG CTTCACTTGG TAGCCCTGTG 
GCATCTGTTT CCTCACCTTC TGCATTTCCA AATGGCAATT CCTGTCCTAC AGGGAATTCT 
GTTAAGGAGT TAGCTTTTCC ACACTGTCTC TCAAATTCCT TCTCTTCTGG GGAGCATTTG 
CTGACTACTA CCCCAGGAAA GACAGGATTC TCAGCCACCA CGAAGACTGT CTTTGAAAGA 
CCTCCATTCA ATTCCCATTT ACAGAAGTCT TTTGTAAGTA GCAACTGGGC TGAAACCCCA 
AGGGTAGAAA AAAGAAATGA AAGCTCTTAT TTCCCAGGAA ATGTTCTTAC AAGCACTGCT 
GTGAAAGATC AGAATAAACA CACTACCTCA ATAAATGACT TGGAAAGAGA AATCCAGGCA 
TCCTGTGATA TTGATAATTT TGACATAGAT GACTTTGATG ATGATGACGA TGACTGGGAA 
AATATGATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGTCTATCA ACCTATTAAG 
GAAGGTCGGC CAGTCAAATC AGTATCAGAA AGGATTTCTT CAGCCAAGAC AGACTCTCTT 
CCAGTGGCAT CTACTGCTCA AAATAAAAAC TTCTCAGAGT CAATTCACAA TTACACTGAC 
AAGTCGGAGC AAAATTTAAC ATCCAGAAAT CTGAAACACA AGCATTTCCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT 
AGAACTAATC AGCTTGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCTTA 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTTCTGGG 
GTCACCATTG TCATTTCCCC CTTGAGATCA CTTATAGTAG ATCAAGTCCA AAAGCTAACT 
TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCCGA AGCTACAAGT 
ATTTACCTCC AGTTATCAAA AAAAGACCCA ATTATAAAAC TTCTATATGT CACTCCAGAG 
AAGGTCTGTG CAAGTAACAG ACTGATTTCT ACTCTGGAGA ATCTGTACAA CAGGAAGCTC 
TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT 
CCAGACTACA AAAGAATGAA TATGCTGCGC CAGAAGTTTC CCTCTGTTCC AGTGATGGCG 
CTTACAGCCA CTGCCAATCC CAGGGTGCAG AAGGACATCC TCACTCAGCT GAAGATTCTC 
AGACCTCAGG TGTTTACCAT GAGCTTTAAC AGACATAATC TAAAATACTA TGTGTTACCC 
AAAAAACCTA AAAAGGTGGC TTTTGATTGC TTAGAATGGA TCAGAAAGCA TCACCCACAT 
GACTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACAATGGC TGACACATTA 
CAGAAAGATG GTCTCGCTGC TCTGGCTTAT CATGCTGGTC TCAGTGACTC TGCCAGAGAT 
GAAGTACAGC ACAAGTGGAT TAATCAGGAT GGCTGCCAGG TTATCTGTGC AACAATTGCC 
TTTGGAATGG GGATTGACAA GCCTGATGTG CGATTTGTGA TCCACGCATC CCTCCCTAAA 
TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATCTCGCAG 
TGCCTGCTCT TTTACACCTA CCACGACGTG ACCAGACTGA AGAGGCTCAT ACTGATGGAA 
AAGGATGGAA ACCATCATAC GAAAGAGACT CACTTCAATA ATTTGTATAG CATGGTACAT 
TACTGTGAAA ATATAGCAGA ATGCAGGAGA ATACAGCTTT TGGCTTACTT TGGTGAAAAT 
GAATTTAATC CTAATTTTTG TAAGAAGTAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA 
ACAAAGGATT ATAAGACAAG AGATGTGACT GATGATGTAA AAAATATTGT AAGATTTGTT 
CAAGAACATA GTTCATCACG AGGAGCAAGA AATATAGGTT CTTCTGGGAG ATTTACTATG 
AATATGCTGG TCGACATTTT CCTGGGGAGT AAAAGTGCAA AACTTCAGTC AGGGATATTT 
GGAAAGGGAT CTGCTTATTC ACGACACAAT GCTGAAAGAC TTTTTAAAAA GCTGATACTT 
GACAAGATTT TGGATGAAGA CTTATATATC AATGCCAATG ATCAACCAAT TGCATATATA 
ATGCTGGGAG ATAAAGCCCA GGCTGTACTG AATGGTCATT TAAAGGTAGA CTTTATGGAA 
ACGGAAAATT CCAGCAGTGT GAAAAAACAA AAGGCTTTAG TGGCAAAGGT ATCTCAGAGG 
GAAGAGATGG TTAAGAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA 
GTTTTTGGTG TCCATTACTT TAACATCTTC AATACCACCA CTCTCAAGAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACAGA AGACAAACTG 
GAAAAATACG GTGCAGAAGT GATTCCAATA TTACAGAAAT ACTCCGAATG GACAGTGCCA 
GCTGAGGACA ATTCCCCACG GAGAAGCCCC TCCAGCTGCA GAGGCAGTGG AAGAAACGCC 
CCCGAGGAGT TGGATGAGGA AACACTCATA TCTTCTCACT ACTTTGCAAA TAAAACCAAA 
AATGAAAGAA AAAGGAAAAG GATACCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTAGT 
TTCAGTGGTT CCAAGGCAAA AGGGGGGTCC GGCACAAGCA GAAGGATATC TTCTAAAACT 
AAACCTTCCA ACGTATTTGG ATCCCGTTCA GCTTTGCTTG GTTCTCAAGC AGCATCAGGA 
GCCAGTAGAA AATTGGGGAT TATGGCTCCA CCAAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCGTATG CATTTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021097883.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020953542.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe222271
Clone ID Related Accession (Same CDS sequence)	XM_021097883.1
Accession Version	XM_021097883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAAG ATCTTGACAC CTCCGACAGA AGGGATGGTC TTGGCACATC AGAGGACTTG 
TCAGAACCTG AGAAAATGAC TTCACAGCAA CCTAATCCAG AAACCAGCAC AAACTGTGAA 
GCTGGACAGA TAAATGTACA GCAGCAGCTT ATCCGTGTGA TGGACCATAT CTGTAAATTA 
CTTGATACCA TTCCTGATGA CAAGCTGAAG GCTTTGGATT GTGGGAATGA ACTGCTGCAG 
CAGCGGGACA TGAGAAGGAA ACTCCTTGCT GAAGCAGATT TTAACACATG TGATGCCAGT 
GCCCTTGCCT TGCTGTGGAG ATGCAGGCCT GCTTCACTTG GTAGCCCTGT GGCATCTGTT 
TCCTCACCTT CTGCATTTCC AAATGGCAAT TCCTGTCCTA CAGGGAATTC TGTTAAGGAG 
TTAGCTTTTC CACACTGTCT CTCAAATTCC TTCTCTTCTG GGGAGCATTT GCTGACTACT 
ACCCCAGGAA AGACAGGATT CTCAGCCACC ACGAAGACTG TCTTTGAAAG ACCTCCATTC 
AATTCCCATT TACAGAAGTC TTTTGTAAGT AGCAACTGGG CTGAAACCCC AAGGGTAGAA 
AAAAGAAATG AAAGCTCTTA TTTCCCAGGA AATGTTCTTA CAAGCACTGC TGTGAAAGAT 
CAGAATAAAC ACACTACCTC AATAAATGAC TTGGAAAGAG AAATCCAGGC ATCCTGTGAT 
ATTGATAATT TTGACATAGA TGACTTTGAT GATGATGACG ATGACTGGGA AAATATGATG 
CATAATTTAG CAGCCAGCAA ATCTTCCACA GCTGTCTATC AACCTATTAA GGAAGGTCGG 
CCAGTCAAAT CAGTATCAGA AAGGATTTCT TCAGCCAAGA CAGACTCTCT TCCAGTGGCA 
TCTACTGCTC AAAATAAAAA CTTCTCAGAG TCAATTCACA ATTACACTGA CAAGTCGGAG 
CAAAATTTAA CATCCAGAAA TCTGAAACAC AAGCATTTCC AAAGTCTTAA TTTTCCTCAT 
ACAAAAGAAA TGATGAAGAT TTTTCATAAA AAATTTGGCC TGCATAATTT TAGAACTAAT 
CAGCTTGAGG CGATCAATGC TGCACTGCTT GGTGAAGACT GTTTTATCTT AATGCCCACT 
GGAGGTGGTA AAAGTTTGTG TTACCAGCTC CCTGCCTGTG TTTCTTCTGG GGTCACCATT 
GTCATTTCCC CCTTGAGATC ACTTATAGTA GATCAAGTCC AAAAGCTAAC TTCCTTGGAT 
ATTCCAGCTA CATATCTGAC AGGTGATAAG ACTGACTCCG AAGCTACAAG TATTTACCTC 
CAGTTATCAA AAAAAGACCC AATTATAAAA CTTCTATATG TCACTCCAGA GAAGGTCTGT 
GCAAGTAACA GACTGATTTC TACTCTGGAG AATCTGTACA ACAGGAAGCT CTTGGCACGT 
TTTGTTATTG ATGAAGCACA TTGTGTCAGT CAGTGGGGAC ATGATTTTCG TCCAGACTAC 
AAAAGAATGA ATATGCTGCG CCAGAAGTTT CCCTCTGTTC CAGTGATGGC GCTTACAGCC 
ACTGCCAATC CCAGGGTGCA GAAGGACATC CTCACTCAGC TGAAGATTCT CAGACCTCAG 
GTGTTTACCA TGAGCTTTAA CAGACATAAT CTAAAATACT ATGTGTTACC CAAAAAACCT 
AAAAAGGTGG CTTTTGATTG CTTAGAATGG ATCAGAAAGC ATCACCCACA TGACTCAGGG 
ATAATTTACT GCCTCTCCAG GCGAGAATGT GACACAATGG CTGACACATT ACAGAAAGAT 
GGTCTCGCTG CTCTGGCTTA TCATGCTGGT CTCAGTGACT CTGCCAGAGA TGAAGTACAG 
CACAAGTGGA TTAATCAGGA TGGCTGCCAG GTTATCTGTG CAACAATTGC CTTTGGAATG 
GGGATTGACA AGCCTGATGT GCGATTTGTG ATCCACGCAT CCCTCCCTAA ATCTGTGGAG 
GGTTACTACC AAGAATCTGG CAGAGCTGGA AGAGATGGGG AAATCTCGCA GTGCCTGCTC 
TTTTACACCT ACCACGACGT GACCAGACTG AAGAGGCTCA TACTGATGGA AAAGGATGGA 
AACCATCATA CGAAAGAGAC TCACTTCAAT AATTTGTATA GCATGGTACA TTACTGTGAA 
AATATAGCAG AATGCAGGAG AATACAGCTT TTGGCTTACT TTGGTGAAAA TGAATTTAAT 
CCTAATTTTT GTAAGAAGTA CCCAGATGTT TCTTGTGATA ATTGCTGTAA AACAAAGGAT 
TATAAGACAA GAGATGTGAC TGATGATGTA AAAAATATTG TAAGATTTGT TCAAGAACAT 
AGTTCATCAC GAGGAGCAAG AAATATAGGT TCTTCTGGGA GATTTACTAT GAATATGCTG 
GTCGACATTT TCCTGGGGAG TAAAAGTGCA AAACTTCAGT CAGGGATATT TGGAAAGGGA 
TCTGCTTATT CACGACACAA TGCTGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT 
TTGGATGAAG ACTTATATAT CAATGCCAAT GATCAACCAA TTGCATATAT AATGCTGGGA 
GATAAAGCCC AGGCTGTACT GAATGGTCAT TTAAAGGTAG ACTTTATGGA AACGGAAAAT 
TCCAGCAGTG TGAAAAAACA AAAGGCTTTA GTGGCAAAGG TATCTCAGAG GGAAGAGATG 
GTTAAGAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT 
GTCCATTACT TTAACATCTT CAATACCACC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT 
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACAG AAGACAAACT GGAAAAATAC 
GGTGCAGAAG TGATTCCAAT ATTACAGAAA TACTCCGAAT GGACAGTGCC AGCTGAGGAC 
AATTCCCCAC GGAGAAGCCC CTCCAGCTGC AGAGGCAGTG GAAGAAACGC CCCCGAGGAG 
TTGGATGAGG AAACACTCAT ATCTTCTCAC TACTTTGCAA ATAAAACCAA AAATGAAAGA 
AAAAGGAAAA GGATACCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTAG TTTCAGTGGT 
TCCAAGGCAA AAGGGGGGTC CGGCACAAGC AGAAGGATAT CTTCTAAAAC TAAACCTTCC 
AACGTATTTG GATCCCGTTC AGCTTTGCTT GGTTCTCAAG CAGCATCAGG AGCCAGTAGA 
AAATTGGGGA TTATGGCTCC ACCAAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCGTAT 
GCATTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020953542.1
CDS	721..4032
Translation	MLKDLDTSDRRDGLGTSEDLSEPEKMTSQQPNPETSTNCEAGQINVQQQLIRVMDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGNSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFSGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_021097883.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021097883.1

ORF Insert Sequence:

ATGTTGAAAG ATCTTGACAC CTCCGACAGA AGGGATGGTC TTGGCACATC AGAGGACTTG 
TCAGAACCTG AGAAAATGAC TTCACAGCAA CCTAATCCAG AAACCAGCAC AAACTGTGAA 
GCTGGACAGA TAAATGTACA GCAGCAGCTT ATCCGTGTGA TGGACCATAT CTGTAAATTA 
CTTGATACCA TTCCTGATGA CAAGCTGAAG GCTTTGGATT GTGGGAATGA ACTGCTGCAG 
CAGCGGGACA TGAGAAGGAA ACTCCTTGCT GAAGCAGATT TTAACACATG TGATGCCAGT 
GCCCTTGCCT TGCTGTGGAG ATGCAGGCCT GCTTCACTTG GTAGCCCTGT GGCATCTGTT 
TCCTCACCTT CTGCATTTCC AAATGGCAAT TCCTGTCCTA CAGGGAATTC TGTTAAGGAG 
TTAGCTTTTC CACACTGTCT CTCAAATTCC TTCTCTTCTG GGGAGCATTT GCTGACTACT 
ACCCCAGGAA AGACAGGATT CTCAGCCACC ACGAAGACTG TCTTTGAAAG ACCTCCATTC 
AATTCCCATT TACAGAAGTC TTTTGTAAGT AGCAACTGGG CTGAAACCCC AAGGGTAGAA 
AAAAGAAATG AAAGCTCTTA TTTCCCAGGA AATGTTCTTA CAAGCACTGC TGTGAAAGAT 
CAGAATAAAC ACACTACCTC AATAAATGAC TTGGAAAGAG AAATCCAGGC ATCCTGTGAT 
ATTGATAATT TTGACATAGA TGACTTTGAT GATGATGACG ATGACTGGGA AAATATGATG 
CATAATTTAG CAGCCAGCAA ATCTTCCACA GCTGTCTATC AACCTATTAA GGAAGGTCGG 
CCAGTCAAAT CAGTATCAGA AAGGATTTCT TCAGCCAAGA CAGACTCTCT TCCAGTGGCA 
TCTACTGCTC AAAATAAAAA CTTCTCAGAG TCAATTCACA ATTACACTGA CAAGTCGGAG 
CAAAATTTAA CATCCAGAAA TCTGAAACAC AAGCATTTCC AAAGTCTTAA TTTTCCTCAT 
ACAAAAGAAA TGATGAAGAT TTTTCATAAA AAATTTGGCC TGCATAATTT TAGAACTAAT 
CAGCTTGAGG CGATCAATGC TGCACTGCTT GGTGAAGACT GTTTTATCTT AATGCCCACT 
GGAGGTGGTA AAAGTTTGTG TTACCAGCTC CCTGCCTGTG TTTCTTCTGG GGTCACCATT 
GTCATTTCCC CCTTGAGATC ACTTATAGTA GATCAAGTCC AAAAGCTAAC TTCCTTGGAT 
ATTCCAGCTA CATATCTGAC AGGTGATAAG ACTGACTCCG AAGCTACAAG TATTTACCTC 
CAGTTATCAA AAAAAGACCC AATTATAAAA CTTCTATATG TCACTCCAGA GAAGGTCTGT 
GCAAGTAACA GACTGATTTC TACTCTGGAG AATCTGTACA ACAGGAAGCT CTTGGCACGT 
TTTGTTATTG ATGAAGCACA TTGTGTCAGT CAGTGGGGAC ATGATTTTCG TCCAGACTAC 
AAAAGAATGA ATATGCTGCG CCAGAAGTTT CCCTCTGTTC CAGTGATGGC GCTTACAGCC 
ACTGCCAATC CCAGGGTGCA GAAGGACATC CTCACTCAGC TGAAGATTCT CAGACCTCAG 
GTGTTTACCA TGAGCTTTAA CAGACATAAT CTAAAATACT ATGTGTTACC CAAAAAACCT 
AAAAAGGTGG CTTTTGATTG CTTAGAATGG ATCAGAAAGC ATCACCCACA TGACTCAGGG 
ATAATTTACT GCCTCTCCAG GCGAGAATGT GACACAATGG CTGACACATT ACAGAAAGAT 
GGTCTCGCTG CTCTGGCTTA TCATGCTGGT CTCAGTGACT CTGCCAGAGA TGAAGTACAG 
CACAAGTGGA TTAATCAGGA TGGCTGCCAG GTTATCTGTG CAACAATTGC CTTTGGAATG 
GGGATTGACA AGCCTGATGT GCGATTTGTG ATCCACGCAT CCCTCCCTAA ATCTGTGGAG 
GGTTACTACC AAGAATCTGG CAGAGCTGGA AGAGATGGGG AAATCTCGCA GTGCCTGCTC 
TTTTACACCT ACCACGACGT GACCAGACTG AAGAGGCTCA TACTGATGGA AAAGGATGGA 
AACCATCATA CGAAAGAGAC TCACTTCAAT AATTTGTATA GCATGGTACA TTACTGTGAA 
AATATAGCAG AATGCAGGAG AATACAGCTT TTGGCTTACT TTGGTGAAAA TGAATTTAAT 
CCTAATTTTT GTAAGAAGTA CCCAGATGTT TCTTGTGATA ATTGCTGTAA AACAAAGGAT 
TATAAGACAA GAGATGTGAC TGATGATGTA AAAAATATTG TAAGATTTGT TCAAGAACAT 
AGTTCATCAC GAGGAGCAAG AAATATAGGT TCTTCTGGGA GATTTACTAT GAATATGCTG 
GTCGACATTT TCCTGGGGAG TAAAAGTGCA AAACTTCAGT CAGGGATATT TGGAAAGGGA 
TCTGCTTATT CACGACACAA TGCTGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT 
TTGGATGAAG ACTTATATAT CAATGCCAAT GATCAACCAA TTGCATATAT AATGCTGGGA 
GATAAAGCCC AGGCTGTACT GAATGGTCAT TTAAAGGTAG ACTTTATGGA AACGGAAAAT 
TCCAGCAGTG TGAAAAAACA AAAGGCTTTA GTGGCAAAGG TATCTCAGAG GGAAGAGATG 
GTTAAGAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT 
GTCCATTACT TTAACATCTT CAATACCACC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT 
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACAG AAGACAAACT GGAAAAATAC 
GGTGCAGAAG TGATTCCAAT ATTACAGAAA TACTCCGAAT GGACAGTGCC AGCTGAGGAC 
AATTCCCCAC GGAGAAGCCC CTCCAGCTGC AGAGGCAGTG GAAGAAACGC CCCCGAGGAG 
TTGGATGAGG AAACACTCAT ATCTTCTCAC TACTTTGCAA ATAAAACCAA AAATGAAAGA 
AAAAGGAAAA GGATACCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTAG TTTCAGTGGT 
TCCAAGGCAA AAGGGGGGTC CGGCACAAGC AGAAGGATAT CTTCTAAAAC TAAACCTTCC 
AACGTATTTG GATCCCGTTC AGCTTTGCTT GGTTCTCAAG CAGCATCAGG AGCCAGTAGA 
AAATTGGGGA TTATGGCTCC ACCAAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCGTAT 
GCATTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021097885.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020953544.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe222272
Clone ID Related Accession (Same CDS sequence)	XM_021097885.1 , XM_021097884.1
Accession Version	XM_021097885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA TCTGTAAATT ACTTGATACC ATTCCTGATG ACAAGCTGAA GGCTTTGGAT 
TGTGGGAATG AACTGCTGCA GCAGCGGGAC ATGAGAAGGA AACTCCTTGC TGAAGCAGAT 
TTTAACACAT GTGATGCCAG TGCCCTTGCC TTGCTGTGGA GATGCAGGCC TGCTTCACTT 
GGTAGCCCTG TGGCATCTGT TTCCTCACCT TCTGCATTTC CAAATGGCAA TTCCTGTCCT 
ACAGGGAATT CTGTTAAGGA GTTAGCTTTT CCACACTGTC TCTCAAATTC CTTCTCTTCT 
GGGGAGCATT TGCTGACTAC TACCCCAGGA AAGACAGGAT TCTCAGCCAC CACGAAGACT 
GTCTTTGAAA GACCTCCATT CAATTCCCAT TTACAGAAGT CTTTTGTAAG TAGCAACTGG 
GCTGAAACCC CAAGGGTAGA AAAAAGAAAT GAAAGCTCTT ATTTCCCAGG AAATGTTCTT 
ACAAGCACTG CTGTGAAAGA TCAGAATAAA CACACTACCT CAATAAATGA CTTGGAAAGA 
GAAATCCAGG CATCCTGTGA TATTGATAAT TTTGACATAG ATGACTTTGA TGATGATGAC 
GATGACTGGG AAAATATGAT GCATAATTTA GCAGCCAGCA AATCTTCCAC AGCTGTCTAT 
CAACCTATTA AGGAAGGTCG GCCAGTCAAA TCAGTATCAG AAAGGATTTC TTCAGCCAAG 
ACAGACTCTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTTCTCAGA GTCAATTCAC 
AATTACACTG ACAAGTCGGA GCAAAATTTA ACATCCAGAA ATCTGAAACA CAAGCATTTC 
CAAAGTCTTA ATTTTCCTCA TACAAAAGAA ATGATGAAGA TTTTTCATAA AAAATTTGGC 
CTGCATAATT TTAGAACTAA TCAGCTTGAG GCGATCAATG CTGCACTGCT TGGTGAAGAC 
TGTTTTATCT TAATGCCCAC TGGAGGTGGT AAAAGTTTGT GTTACCAGCT CCCTGCCTGT 
GTTTCTTCTG GGGTCACCAT TGTCATTTCC CCCTTGAGAT CACTTATAGT AGATCAAGTC 
CAAAAGCTAA CTTCCTTGGA TATTCCAGCT ACATATCTGA CAGGTGATAA GACTGACTCC 
GAAGCTACAA GTATTTACCT CCAGTTATCA AAAAAAGACC CAATTATAAA ACTTCTATAT 
GTCACTCCAG AGAAGGTCTG TGCAAGTAAC AGACTGATTT CTACTCTGGA GAATCTGTAC 
AACAGGAAGC TCTTGGCACG TTTTGTTATT GATGAAGCAC ATTGTGTCAG TCAGTGGGGA 
CATGATTTTC GTCCAGACTA CAAAAGAATG AATATGCTGC GCCAGAAGTT TCCCTCTGTT 
CCAGTGATGG CGCTTACAGC CACTGCCAAT CCCAGGGTGC AGAAGGACAT CCTCACTCAG 
CTGAAGATTC TCAGACCTCA GGTGTTTACC ATGAGCTTTA ACAGACATAA TCTAAAATAC 
TATGTGTTAC CCAAAAAACC TAAAAAGGTG GCTTTTGATT GCTTAGAATG GATCAGAAAG 
CATCACCCAC ATGACTCAGG GATAATTTAC TGCCTCTCCA GGCGAGAATG TGACACAATG 
GCTGACACAT TACAGAAAGA TGGTCTCGCT GCTCTGGCTT ATCATGCTGG TCTCAGTGAC 
TCTGCCAGAG ATGAAGTACA GCACAAGTGG ATTAATCAGG ATGGCTGCCA GGTTATCTGT 
GCAACAATTG CCTTTGGAAT GGGGATTGAC AAGCCTGATG TGCGATTTGT GATCCACGCA 
TCCCTCCCTA AATCTGTGGA GGGTTACTAC CAAGAATCTG GCAGAGCTGG AAGAGATGGG 
GAAATCTCGC AGTGCCTGCT CTTTTACACC TACCACGACG TGACCAGACT GAAGAGGCTC 
ATACTGATGG AAAAGGATGG AAACCATCAT ACGAAAGAGA CTCACTTCAA TAATTTGTAT 
AGCATGGTAC ATTACTGTGA AAATATAGCA GAATGCAGGA GAATACAGCT TTTGGCTTAC 
TTTGGTGAAA ATGAATTTAA TCCTAATTTT TGTAAGAAGT ACCCAGATGT TTCTTGTGAT 
AATTGCTGTA AAACAAAGGA TTATAAGACA AGAGATGTGA CTGATGATGT AAAAAATATT 
GTAAGATTTG TTCAAGAACA TAGTTCATCA CGAGGAGCAA GAAATATAGG TTCTTCTGGG 
AGATTTACTA TGAATATGCT GGTCGACATT TTCCTGGGGA GTAAAAGTGC AAAACTTCAG 
TCAGGGATAT TTGGAAAGGG ATCTGCTTAT TCACGACACA ATGCTGAAAG ACTTTTTAAA 
AAGCTGATAC TTGACAAGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAACCA 
ATTGCATATA TAATGCTGGG AGATAAAGCC CAGGCTGTAC TGAATGGTCA TTTAAAGGTA 
GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAGGCTTT AGTGGCAAAG 
GTATCTCAGA GGGAAGAGAT GGTTAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA 
TCTCTGGGGA AAGTTTTTGG TGTCCATTAC TTTAACATCT TCAATACCAC CACTCTCAAG 
AAGCTTGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACA 
GAAGACAAAC TGGAAAAATA CGGTGCAGAA GTGATTCCAA TATTACAGAA ATACTCCGAA 
TGGACAGTGC CAGCTGAGGA CAATTCCCCA CGGAGAAGCC CCTCCAGCTG CAGAGGCAGT 
GGAAGAAACG CCCCCGAGGA GTTGGATGAG GAAACACTCA TATCTTCTCA CTACTTTGCA 
AATAAAACCA AAAATGAAAG AAAAAGGAAA AGGATACCAG CCTCCCAAAG GTCTAAGAGG 
AGAAAAACTA GTTTCAGTGG TTCCAAGGCA AAAGGGGGGT CCGGCACAAG CAGAAGGATA 
TCTTCTAAAA CTAAACCTTC CAACGTATTT GGATCCCGTT CAGCTTTGCT TGGTTCTCAA 
GCAGCATCAG GAGCCAGTAG AAAATTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA 
CCGTTTCTTA AGCCTTCGTA TGCATTTTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020953544.1
CDS	1078..4230
Translation	MDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGNSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFSGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_021097885.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021097885.1

ORF Insert Sequence:

ATGGACCATA TCTGTAAATT ACTTGATACC ATTCCTGATG ACAAGCTGAA GGCTTTGGAT 
TGTGGGAATG AACTGCTGCA GCAGCGGGAC ATGAGAAGGA AACTCCTTGC TGAAGCAGAT 
TTTAACACAT GTGATGCCAG TGCCCTTGCC TTGCTGTGGA GATGCAGGCC TGCTTCACTT 
GGTAGCCCTG TGGCATCTGT TTCCTCACCT TCTGCATTTC CAAATGGCAA TTCCTGTCCT 
ACAGGGAATT CTGTTAAGGA GTTAGCTTTT CCACACTGTC TCTCAAATTC CTTCTCTTCT 
GGGGAGCATT TGCTGACTAC TACCCCAGGA AAGACAGGAT TCTCAGCCAC CACGAAGACT 
GTCTTTGAAA GACCTCCATT CAATTCCCAT TTACAGAAGT CTTTTGTAAG TAGCAACTGG 
GCTGAAACCC CAAGGGTAGA AAAAAGAAAT GAAAGCTCTT ATTTCCCAGG AAATGTTCTT 
ACAAGCACTG CTGTGAAAGA TCAGAATAAA CACACTACCT CAATAAATGA CTTGGAAAGA 
GAAATCCAGG CATCCTGTGA TATTGATAAT TTTGACATAG ATGACTTTGA TGATGATGAC 
GATGACTGGG AAAATATGAT GCATAATTTA GCAGCCAGCA AATCTTCCAC AGCTGTCTAT 
CAACCTATTA AGGAAGGTCG GCCAGTCAAA TCAGTATCAG AAAGGATTTC TTCAGCCAAG 
ACAGACTCTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTTCTCAGA GTCAATTCAC 
AATTACACTG ACAAGTCGGA GCAAAATTTA ACATCCAGAA ATCTGAAACA CAAGCATTTC 
CAAAGTCTTA ATTTTCCTCA TACAAAAGAA ATGATGAAGA TTTTTCATAA AAAATTTGGC 
CTGCATAATT TTAGAACTAA TCAGCTTGAG GCGATCAATG CTGCACTGCT TGGTGAAGAC 
TGTTTTATCT TAATGCCCAC TGGAGGTGGT AAAAGTTTGT GTTACCAGCT CCCTGCCTGT 
GTTTCTTCTG GGGTCACCAT TGTCATTTCC CCCTTGAGAT CACTTATAGT AGATCAAGTC 
CAAAAGCTAA CTTCCTTGGA TATTCCAGCT ACATATCTGA CAGGTGATAA GACTGACTCC 
GAAGCTACAA GTATTTACCT CCAGTTATCA AAAAAAGACC CAATTATAAA ACTTCTATAT 
GTCACTCCAG AGAAGGTCTG TGCAAGTAAC AGACTGATTT CTACTCTGGA GAATCTGTAC 
AACAGGAAGC TCTTGGCACG TTTTGTTATT GATGAAGCAC ATTGTGTCAG TCAGTGGGGA 
CATGATTTTC GTCCAGACTA CAAAAGAATG AATATGCTGC GCCAGAAGTT TCCCTCTGTT 
CCAGTGATGG CGCTTACAGC CACTGCCAAT CCCAGGGTGC AGAAGGACAT CCTCACTCAG 
CTGAAGATTC TCAGACCTCA GGTGTTTACC ATGAGCTTTA ACAGACATAA TCTAAAATAC 
TATGTGTTAC CCAAAAAACC TAAAAAGGTG GCTTTTGATT GCTTAGAATG GATCAGAAAG 
CATCACCCAC ATGACTCAGG GATAATTTAC TGCCTCTCCA GGCGAGAATG TGACACAATG 
GCTGACACAT TACAGAAAGA TGGTCTCGCT GCTCTGGCTT ATCATGCTGG TCTCAGTGAC 
TCTGCCAGAG ATGAAGTACA GCACAAGTGG ATTAATCAGG ATGGCTGCCA GGTTATCTGT 
GCAACAATTG CCTTTGGAAT GGGGATTGAC AAGCCTGATG TGCGATTTGT GATCCACGCA 
TCCCTCCCTA AATCTGTGGA GGGTTACTAC CAAGAATCTG GCAGAGCTGG AAGAGATGGG 
GAAATCTCGC AGTGCCTGCT CTTTTACACC TACCACGACG TGACCAGACT GAAGAGGCTC 
ATACTGATGG AAAAGGATGG AAACCATCAT ACGAAAGAGA CTCACTTCAA TAATTTGTAT 
AGCATGGTAC ATTACTGTGA AAATATAGCA GAATGCAGGA GAATACAGCT TTTGGCTTAC 
TTTGGTGAAA ATGAATTTAA TCCTAATTTT TGTAAGAAGT ACCCAGATGT TTCTTGTGAT 
AATTGCTGTA AAACAAAGGA TTATAAGACA AGAGATGTGA CTGATGATGT AAAAAATATT 
GTAAGATTTG TTCAAGAACA TAGTTCATCA CGAGGAGCAA GAAATATAGG TTCTTCTGGG 
AGATTTACTA TGAATATGCT GGTCGACATT TTCCTGGGGA GTAAAAGTGC AAAACTTCAG 
TCAGGGATAT TTGGAAAGGG ATCTGCTTAT TCACGACACA ATGCTGAAAG ACTTTTTAAA 
AAGCTGATAC TTGACAAGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAACCA 
ATTGCATATA TAATGCTGGG AGATAAAGCC CAGGCTGTAC TGAATGGTCA TTTAAAGGTA 
GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAGGCTTT AGTGGCAAAG 
GTATCTCAGA GGGAAGAGAT GGTTAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA 
TCTCTGGGGA AAGTTTTTGG TGTCCATTAC TTTAACATCT TCAATACCAC CACTCTCAAG 
AAGCTTGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACA 
GAAGACAAAC TGGAAAAATA CGGTGCAGAA GTGATTCCAA TATTACAGAA ATACTCCGAA 
TGGACAGTGC CAGCTGAGGA CAATTCCCCA CGGAGAAGCC CCTCCAGCTG CAGAGGCAGT 
GGAAGAAACG CCCCCGAGGA GTTGGATGAG GAAACACTCA TATCTTCTCA CTACTTTGCA 
AATAAAACCA AAAATGAAAG AAAAAGGAAA AGGATACCAG CCTCCCAAAG GTCTAAGAGG 
AGAAAAACTA GTTTCAGTGG TTCCAAGGCA AAAGGGGGGT CCGGCACAAG CAGAAGGATA 
TCTTCTAAAA CTAAACCTTC CAACGTATTT GGATCCCGTT CAGCTTTGCT TGGTTCTCAA 
GCAGCATCAG GAGCCAGTAG AAAATTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA 
CCGTTTCTTA AGCCTTCGTA TGCATTTTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021097884.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020953543.1 Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X4, mRNA.

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CloneID	OSe222272
Clone ID Related Accession (Same CDS sequence)	XM_021097885.1 , XM_021097884.1
Accession Version	XM_021097884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	Bloom syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA TCTGTAAATT ACTTGATACC ATTCCTGATG ACAAGCTGAA GGCTTTGGAT 
TGTGGGAATG AACTGCTGCA GCAGCGGGAC ATGAGAAGGA AACTCCTTGC TGAAGCAGAT 
TTTAACACAT GTGATGCCAG TGCCCTTGCC TTGCTGTGGA GATGCAGGCC TGCTTCACTT 
GGTAGCCCTG TGGCATCTGT TTCCTCACCT TCTGCATTTC CAAATGGCAA TTCCTGTCCT 
ACAGGGAATT CTGTTAAGGA GTTAGCTTTT CCACACTGTC TCTCAAATTC CTTCTCTTCT 
GGGGAGCATT TGCTGACTAC TACCCCAGGA AAGACAGGAT TCTCAGCCAC CACGAAGACT 
GTCTTTGAAA GACCTCCATT CAATTCCCAT TTACAGAAGT CTTTTGTAAG TAGCAACTGG 
GCTGAAACCC CAAGGGTAGA AAAAAGAAAT GAAAGCTCTT ATTTCCCAGG AAATGTTCTT 
ACAAGCACTG CTGTGAAAGA TCAGAATAAA CACACTACCT CAATAAATGA CTTGGAAAGA 
GAAATCCAGG CATCCTGTGA TATTGATAAT TTTGACATAG ATGACTTTGA TGATGATGAC 
GATGACTGGG AAAATATGAT GCATAATTTA GCAGCCAGCA AATCTTCCAC AGCTGTCTAT 
CAACCTATTA AGGAAGGTCG GCCAGTCAAA TCAGTATCAG AAAGGATTTC TTCAGCCAAG 
ACAGACTCTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTTCTCAGA GTCAATTCAC 
AATTACACTG ACAAGTCGGA GCAAAATTTA ACATCCAGAA ATCTGAAACA CAAGCATTTC 
CAAAGTCTTA ATTTTCCTCA TACAAAAGAA ATGATGAAGA TTTTTCATAA AAAATTTGGC 
CTGCATAATT TTAGAACTAA TCAGCTTGAG GCGATCAATG CTGCACTGCT TGGTGAAGAC 
TGTTTTATCT TAATGCCCAC TGGAGGTGGT AAAAGTTTGT GTTACCAGCT CCCTGCCTGT 
GTTTCTTCTG GGGTCACCAT TGTCATTTCC CCCTTGAGAT CACTTATAGT AGATCAAGTC 
CAAAAGCTAA CTTCCTTGGA TATTCCAGCT ACATATCTGA CAGGTGATAA GACTGACTCC 
GAAGCTACAA GTATTTACCT CCAGTTATCA AAAAAAGACC CAATTATAAA ACTTCTATAT 
GTCACTCCAG AGAAGGTCTG TGCAAGTAAC AGACTGATTT CTACTCTGGA GAATCTGTAC 
AACAGGAAGC TCTTGGCACG TTTTGTTATT GATGAAGCAC ATTGTGTCAG TCAGTGGGGA 
CATGATTTTC GTCCAGACTA CAAAAGAATG AATATGCTGC GCCAGAAGTT TCCCTCTGTT 
CCAGTGATGG CGCTTACAGC CACTGCCAAT CCCAGGGTGC AGAAGGACAT CCTCACTCAG 
CTGAAGATTC TCAGACCTCA GGTGTTTACC ATGAGCTTTA ACAGACATAA TCTAAAATAC 
TATGTGTTAC CCAAAAAACC TAAAAAGGTG GCTTTTGATT GCTTAGAATG GATCAGAAAG 
CATCACCCAC ATGACTCAGG GATAATTTAC TGCCTCTCCA GGCGAGAATG TGACACAATG 
GCTGACACAT TACAGAAAGA TGGTCTCGCT GCTCTGGCTT ATCATGCTGG TCTCAGTGAC 
TCTGCCAGAG ATGAAGTACA GCACAAGTGG ATTAATCAGG ATGGCTGCCA GGTTATCTGT 
GCAACAATTG CCTTTGGAAT GGGGATTGAC AAGCCTGATG TGCGATTTGT GATCCACGCA 
TCCCTCCCTA AATCTGTGGA GGGTTACTAC CAAGAATCTG GCAGAGCTGG AAGAGATGGG 
GAAATCTCGC AGTGCCTGCT CTTTTACACC TACCACGACG TGACCAGACT GAAGAGGCTC 
ATACTGATGG AAAAGGATGG AAACCATCAT ACGAAAGAGA CTCACTTCAA TAATTTGTAT 
AGCATGGTAC ATTACTGTGA AAATATAGCA GAATGCAGGA GAATACAGCT TTTGGCTTAC 
TTTGGTGAAA ATGAATTTAA TCCTAATTTT TGTAAGAAGT ACCCAGATGT TTCTTGTGAT 
AATTGCTGTA AAACAAAGGA TTATAAGACA AGAGATGTGA CTGATGATGT AAAAAATATT 
GTAAGATTTG TTCAAGAACA TAGTTCATCA CGAGGAGCAA GAAATATAGG TTCTTCTGGG 
AGATTTACTA TGAATATGCT GGTCGACATT TTCCTGGGGA GTAAAAGTGC AAAACTTCAG 
TCAGGGATAT TTGGAAAGGG ATCTGCTTAT TCACGACACA ATGCTGAAAG ACTTTTTAAA 
AAGCTGATAC TTGACAAGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAACCA 
ATTGCATATA TAATGCTGGG AGATAAAGCC CAGGCTGTAC TGAATGGTCA TTTAAAGGTA 
GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAGGCTTT AGTGGCAAAG 
GTATCTCAGA GGGAAGAGAT GGTTAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA 
TCTCTGGGGA AAGTTTTTGG TGTCCATTAC TTTAACATCT TCAATACCAC CACTCTCAAG 
AAGCTTGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACA 
GAAGACAAAC TGGAAAAATA CGGTGCAGAA GTGATTCCAA TATTACAGAA ATACTCCGAA 
TGGACAGTGC CAGCTGAGGA CAATTCCCCA CGGAGAAGCC CCTCCAGCTG CAGAGGCAGT 
GGAAGAAACG CCCCCGAGGA GTTGGATGAG GAAACACTCA TATCTTCTCA CTACTTTGCA 
AATAAAACCA AAAATGAAAG AAAAAGGAAA AGGATACCAG CCTCCCAAAG GTCTAAGAGG 
AGAAAAACTA GTTTCAGTGG TTCCAAGGCA AAAGGGGGGT CCGGCACAAG CAGAAGGATA 
TCTTCTAAAA CTAAACCTTC CAACGTATTT GGATCCCGTT CAGCTTTGCT TGGTTCTCAA 
GCAGCATCAG GAGCCAGTAG AAAATTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA 
CCGTTTCTTA AGCCTTCGTA TGCATTTTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020953543.1
CDS	1272..4424
Translation	MDHICKLLDTIPDDKLKALDCGNELLQQRDMRRKLLAEADFNTCDASALALLWRCRPASLGSPVASVSSPSAFPNGNSCPTGNSVKELAFPHCLSNSFSSGEHLLTTTPGKTGFSATTKTVFERPPFNSHLQKSFVSSNWAETPRVEKRNESSYFPGNVLTSTAVKDQNKHTTSINDLEREIQASCDIDNFDIDDFDDDDDDWENMMHNLAASKSSTAVYQPIKEGRPVKSVSERISSAKTDSLPVASTAQNKNFSESIHNYTDKSEQNLTSRNLKHKHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSSGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYNRKLLARFVIDEAHCVSQWGHDFRPDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFTMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPHDSGIIYCLSRRECDTMADTLQKDGLAALAYHAGLSDSARDEVQHKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISQCLLFYTYHDVTRLKRLILMEKDGNHHTKETHFNNLYSMVHYCENIAECRRIQLLAYFGENEFNPNFCKKYPDVSCDNCCKTKDYKTRDVTDDVKNIVRFVQEHSSSRGARNIGSSGRFTMNMLVDIFLGSKSAKLQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYIMLGDKAQAVLNGHLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTTTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPILQKYSEWTVPAEDNSPRRSPSSCRGSGRNAPEELDEETLISSHYFANKTKNERKRKRIPASQRSKRRKTSFSGSKAKGGSGTSRRISSKTKPSNVFGSRSALLGSQAASGASRKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_021097884.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Bloom syndrome RecQ like helicase (BLM), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021097884.1

ORF Insert Sequence:

ATGGACCATA TCTGTAAATT ACTTGATACC ATTCCTGATG ACAAGCTGAA GGCTTTGGAT 
TGTGGGAATG AACTGCTGCA GCAGCGGGAC ATGAGAAGGA AACTCCTTGC TGAAGCAGAT 
TTTAACACAT GTGATGCCAG TGCCCTTGCC TTGCTGTGGA GATGCAGGCC TGCTTCACTT 
GGTAGCCCTG TGGCATCTGT TTCCTCACCT TCTGCATTTC CAAATGGCAA TTCCTGTCCT 
ACAGGGAATT CTGTTAAGGA GTTAGCTTTT CCACACTGTC TCTCAAATTC CTTCTCTTCT 
GGGGAGCATT TGCTGACTAC TACCCCAGGA AAGACAGGAT TCTCAGCCAC CACGAAGACT 
GTCTTTGAAA GACCTCCATT CAATTCCCAT TTACAGAAGT CTTTTGTAAG TAGCAACTGG 
GCTGAAACCC CAAGGGTAGA AAAAAGAAAT GAAAGCTCTT ATTTCCCAGG AAATGTTCTT 
ACAAGCACTG CTGTGAAAGA TCAGAATAAA CACACTACCT CAATAAATGA CTTGGAAAGA 
GAAATCCAGG CATCCTGTGA TATTGATAAT TTTGACATAG ATGACTTTGA TGATGATGAC 
GATGACTGGG AAAATATGAT GCATAATTTA GCAGCCAGCA AATCTTCCAC AGCTGTCTAT 
CAACCTATTA AGGAAGGTCG GCCAGTCAAA TCAGTATCAG AAAGGATTTC TTCAGCCAAG 
ACAGACTCTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTTCTCAGA GTCAATTCAC 
AATTACACTG ACAAGTCGGA GCAAAATTTA ACATCCAGAA ATCTGAAACA CAAGCATTTC 
CAAAGTCTTA ATTTTCCTCA TACAAAAGAA ATGATGAAGA TTTTTCATAA AAAATTTGGC 
CTGCATAATT TTAGAACTAA TCAGCTTGAG GCGATCAATG CTGCACTGCT TGGTGAAGAC 
TGTTTTATCT TAATGCCCAC TGGAGGTGGT AAAAGTTTGT GTTACCAGCT CCCTGCCTGT 
GTTTCTTCTG GGGTCACCAT TGTCATTTCC CCCTTGAGAT CACTTATAGT AGATCAAGTC 
CAAAAGCTAA CTTCCTTGGA TATTCCAGCT ACATATCTGA CAGGTGATAA GACTGACTCC 
GAAGCTACAA GTATTTACCT CCAGTTATCA AAAAAAGACC CAATTATAAA ACTTCTATAT 
GTCACTCCAG AGAAGGTCTG TGCAAGTAAC AGACTGATTT CTACTCTGGA GAATCTGTAC 
AACAGGAAGC TCTTGGCACG TTTTGTTATT GATGAAGCAC ATTGTGTCAG TCAGTGGGGA 
CATGATTTTC GTCCAGACTA CAAAAGAATG AATATGCTGC GCCAGAAGTT TCCCTCTGTT 
CCAGTGATGG CGCTTACAGC CACTGCCAAT CCCAGGGTGC AGAAGGACAT CCTCACTCAG 
CTGAAGATTC TCAGACCTCA GGTGTTTACC ATGAGCTTTA ACAGACATAA TCTAAAATAC 
TATGTGTTAC CCAAAAAACC TAAAAAGGTG GCTTTTGATT GCTTAGAATG GATCAGAAAG 
CATCACCCAC ATGACTCAGG GATAATTTAC TGCCTCTCCA GGCGAGAATG TGACACAATG 
GCTGACACAT TACAGAAAGA TGGTCTCGCT GCTCTGGCTT ATCATGCTGG TCTCAGTGAC 
TCTGCCAGAG ATGAAGTACA GCACAAGTGG ATTAATCAGG ATGGCTGCCA GGTTATCTGT 
GCAACAATTG CCTTTGGAAT GGGGATTGAC AAGCCTGATG TGCGATTTGT GATCCACGCA 
TCCCTCCCTA AATCTGTGGA GGGTTACTAC CAAGAATCTG GCAGAGCTGG AAGAGATGGG 
GAAATCTCGC AGTGCCTGCT CTTTTACACC TACCACGACG TGACCAGACT GAAGAGGCTC 
ATACTGATGG AAAAGGATGG AAACCATCAT ACGAAAGAGA CTCACTTCAA TAATTTGTAT 
AGCATGGTAC ATTACTGTGA AAATATAGCA GAATGCAGGA GAATACAGCT TTTGGCTTAC 
TTTGGTGAAA ATGAATTTAA TCCTAATTTT TGTAAGAAGT ACCCAGATGT TTCTTGTGAT 
AATTGCTGTA AAACAAAGGA TTATAAGACA AGAGATGTGA CTGATGATGT AAAAAATATT 
GTAAGATTTG TTCAAGAACA TAGTTCATCA CGAGGAGCAA GAAATATAGG TTCTTCTGGG 
AGATTTACTA TGAATATGCT GGTCGACATT TTCCTGGGGA GTAAAAGTGC AAAACTTCAG 
TCAGGGATAT TTGGAAAGGG ATCTGCTTAT TCACGACACA ATGCTGAAAG ACTTTTTAAA 
AAGCTGATAC TTGACAAGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAACCA 
ATTGCATATA TAATGCTGGG AGATAAAGCC CAGGCTGTAC TGAATGGTCA TTTAAAGGTA 
GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAGGCTTT AGTGGCAAAG 
GTATCTCAGA GGGAAGAGAT GGTTAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA 
TCTCTGGGGA AAGTTTTTGG TGTCCATTAC TTTAACATCT TCAATACCAC CACTCTCAAG 
AAGCTTGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACA 
GAAGACAAAC TGGAAAAATA CGGTGCAGAA GTGATTCCAA TATTACAGAA ATACTCCGAA 
TGGACAGTGC CAGCTGAGGA CAATTCCCCA CGGAGAAGCC CCTCCAGCTG CAGAGGCAGT 
GGAAGAAACG CCCCCGAGGA GTTGGATGAG GAAACACTCA TATCTTCTCA CTACTTTGCA 
AATAAAACCA AAAATGAAAG AAAAAGGAAA AGGATACCAG CCTCCCAAAG GTCTAAGAGG 
AGAAAAACTA GTTTCAGTGG TTCCAAGGCA AAAGGGGGGT CCGGCACAAG CAGAAGGATA 
TCTTCTAAAA CTAAACCTTC CAACGTATTT GGATCCCGTT CAGCTTTGCT TGGTTCTCAA 
GCAGCATCAG GAGCCAGTAG AAAATTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA 
CCGTTTCTTA AGCCTTCGTA TGCATTTTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Isolation and sequencing of pig Blm and Ubl-1/SUMO-1 genes that relate to the recombination machinery.
Transplantation proceedings40(8)2773-5(2008 Oct)
Matsunami K,Otsuka H,Xu H,Furidaus S,Ishimaru A,Fukuzawa M,Miyagawa S

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BLM cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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