LOC578189 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC578189
Entrez Gene ID	578189
Full Name	remodeling and spacing factor 1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003725608.2	XP_003725656.1	remodeling and spacing factor 1 isoform X1
XM_003725608.3	XP_003725656.2	remodeling and spacing factor 1
XM_778377.4	XP_783470.2	remodeling and spacing factor 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC578189 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC578189 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd06091	XM_003725608.2 Latest version!	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd06092	XM_778377.4 Latest version!	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd47991	XM_003725608.3 Latest version!	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC578189 ( XM_003725608.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_003725656.1 Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd06091
Clone ID Related Accession (Same CDS sequence)	XM_003725608.2
Accession Version	XM_003725608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011991989.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_003725608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AGTTGATAGT CAATCTGCAA GCAAAACTGG AAGATCTCGA TGGTGACCTG 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACAG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCGAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGACGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGTCGAGGG AAGGACATGT CAACCATCAT GGGAGACGAA 
GTTGACGAGG AGGAGGAGCA GGAGGGGGAG GAGACGGGGG AGAAGAGAGC CGAGGAGGTG 
GACGGAGAAG CTGAAGAGGG GAAGAGGCGG GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGTGAAG ACTTCAAGGT GGACAGTGAG 
AATGAGAGTG AGTCGGCTGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCAAGACGA AAGGCACCCT CGAAGAGGAG CCTTCGCTAC 
GCTGGCATGC CGCGAAGAAA GAGTGAGAGG GGGAGGAAGA GGGTGAGATA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG AGGAGGAGGG AGAGGAGAAG 
ACAAAATCGA AGAGAAGGAT TGAAAGTGAC AGTGAGGATA GTGATATTGG AAGGAGGAAA 
AAGAAGAAGA ATAGCATCGA TCTCCAGCGA TCTTCTCTGC GGAAGCTATC CACAAGGATC 
GACTATAAGA AAATGGCGGG GGAGAGCACA GAATCGGACG AAAATGATAG TAAGAGTGAA 
CGGGATCAGG ACTCAAACAA GGAGAATGAG GATGAATCTG GCGACGAAAG CAGTGATGAG 
GCTGTTAGCC AGAAAGTGAG GACTACCAAA CAACTCCCCC ATTCGCAAAT GGGACAATCT 
TACATTCCAA CCACTGTCAA TGGGGGTGCT AGTACAGGAG GCCTTCACGA CGGGGCTGCT 
TCACCGGGGG AGAGGGATGC TCATGGTGGC GCAATCGGAG GGACTGTAAA TGGAGAAGGC 
GAGGAGGAGG ACGACCTTTT ACAGGTCACC GACCTGATCG ACTATGTGAC ACAAGACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003725656.1
CDS	24..4946
Translation	MASVHKFPDFAVICSFLERYGEMLQLPDLTIPELQEAIEETKCDVPILREMIIKLMRRLIKNVNAEKWERHLVKISRYYSGMAAWEVDTLGYMQSKTETKLGLLKFLCDSQFDEPKSKFRLAVNEIEPETMRIQPIGRDKMGLVYWFQKDHDANIRVYREEQDDLDNSSWQMVSQDRDDLAGLLAVLKKGTNKLTSLELKTDDSESTTTSVTNDEDTELDIKEEIAKLRLKVSFRQETDSEGVKLETTMQAEVMKPEGTVKSETLAVKEEQMDTTDGQKEYIDDKKGVKEEPMESCDSKENLSKLKTEMETESSVQKDDCVASKLEEATKIKSEEGMVDREGLVDVKKEELGVKQEVPPCRRGSVIMKVGDGVKGYKPVSTPSGSEASVMKDDKEDGDAKAIAGESESKESNKSTDPSEKVLERAPGKGNSSKEEKEKSKDIVSDERTREDQDGEQGRPEGGSTSAVVPEEVSERAPRPASAPELTVTPPKTLAGEPMASSSTSSLIPSQASPRARTPTTSLFMRPWETAPAQPEKEMEEVEGEGTSVGVDSSRKDGQVPAQSKGGLEGKGSVAKQTQLQEDEGCKDNYSQSIPEVRKLVDVPKPKEDNPALEKSVQEKEATEKEEDRSARADPLENTNMSVTQEKGQETEVKDVVNKMEVSKDKSGPDRDAKVGKGVDLQEKDSKKSPAEAGVERSEGEGSRKRDGMKEKEKRKEAEEEDGKVATSEEDEDLLKGDGKVSGVEQHDESDGKTKCAEETDPKDQSDGKEKTGESKEEGSKVEEDVTADRPASKEMEELSEEGAKRETESTEKIDEEEKEDDGVEKDKTEAEEAVQPLRKGSRPLRPRRGKQQEEQPAETKKPKPAPKKRGRPKKKVEVSSQDEKTSQDEGFSQEDEEEKESKKKSISKELMEDEEEDEEDDEEEEEESDDDDNDDDYGRKKIQKKKGVRTAKKKRKKKRLRYTPKRKASQKAVNKIVEMEKDEDELPAKSSYAGKNKLKKADDSDWISEENDDTPCCKCGLYNHPRWILLCDKCDSGFHTACLRPPLMAIPDGNWFCPKCEHEELIVNLQAKLEDLDGDLKKKDRLARRDERKLKYKYTDVCAVNILPEDTTVVEKKKRSRRNDYEEEEDEEEEVEYEDQRNHRSHSSRSRRSQGSRSRRSQGSRRRRSRSSRHHRDRRSRPRRSQRDFVVPDFEDDYEVVSRRSQRSRKTIDYSFNDFDSAIKTAINDEVEEHKKAVDQIKSRWGVSRGKDMSTIMGDEVDEEEEQEGEETGEKRAEEVDGEAEEGKRRDRKGKKKRRLNDLDGSSEDENSEDFKVDSENESESAEDGSNFSEASEDSFLTSRRRASRRKAPSKRSLRYAGMPRRKSERGRKRVRYYSSAEDDEGSYYSGSSGGSYTGSDSEDDEGTSGGRRKAAAKVISYREYSDSDEGRLKKRKQAARTPTPSPSSSSEVEDYTQRKRKFRIDSDEEEEEEEEGEEKTKSKRRIESDSEDSDIGRRKKKKNSIDLQRSSLRKLSTRIDYKKMAGESTESDENDSKSERDQDSNKENEDESGDESSDEAVSQKVRTTKQLPHSQMGQSYIPTTVNGGASTGGLHDGAASPGERDAHGGAIGGTVNGEGEEEDDLLQVTDLIDYVTQDI

Target ORF information:

RefSeq Version	XM_003725608.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003725608.2

ORF Insert Sequence:

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AGTTGATAGT CAATCTGCAA GCAAAACTGG AAGATCTCGA TGGTGACCTG 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACAG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCGAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGACGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGTCGAGGG AAGGACATGT CAACCATCAT GGGAGACGAA 
GTTGACGAGG AGGAGGAGCA GGAGGGGGAG GAGACGGGGG AGAAGAGAGC CGAGGAGGTG 
GACGGAGAAG CTGAAGAGGG GAAGAGGCGG GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGTGAAG ACTTCAAGGT GGACAGTGAG 
AATGAGAGTG AGTCGGCTGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCAAGACGA AAGGCACCCT CGAAGAGGAG CCTTCGCTAC 
GCTGGCATGC CGCGAAGAAA GAGTGAGAGG GGGAGGAAGA GGGTGAGATA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG AGGAGGAGGG AGAGGAGAAG 
ACAAAATCGA AGAGAAGGAT TGAAAGTGAC AGTGAGGATA GTGATATTGG AAGGAGGAAA 
AAGAAGAAGA ATAGCATCGA TCTCCAGCGA TCTTCTCTGC GGAAGCTATC CACAAGGATC 
GACTATAAGA AAATGGCGGG GGAGAGCACA GAATCGGACG AAAATGATAG TAAGAGTGAA 
CGGGATCAGG ACTCAAACAA GGAGAATGAG GATGAATCTG GCGACGAAAG CAGTGATGAG 
GCTGTTAGCC AGAAAGTGAG GACTACCAAA CAACTCCCCC ATTCGCAAAT GGGACAATCT 
TACATTCCAA CCACTGTCAA TGGGGGTGCT AGTACAGGAG GCCTTCACGA CGGGGCTGCT 
TCACCGGGGG AGAGGGATGC TCATGGTGGC GCAATCGGAG GGACTGTAAA TGGAGAAGGC 
GAGGAGGAGG ACGACCTTTT ACAGGTCACC GACCTGATCG ACTATGTGAC ACAAGACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC578189 ( XM_778377.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_783470.2 Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd06092
Clone ID Related Accession (Same CDS sequence)	XM_778377.4
Accession Version	XM_778377.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	remodeling and spacing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011991989.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_778377.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AACTGATAGT CAATCTGCAA GCAAAGCTGG AAGATCTCGA TGGTGACCTC 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACAG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCGAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGACGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGTCGAGGG AAGGACATGT CAACCATCAT GGGAGACGAA 
GTTGACGAGG AGGAGGAGCA GGAGGGGGAG GAGACGGGGG AGAAGAGAGC CGAGGAGGTG 
GACGGAGAAG CTGAAGAGGG GAAGAGGCGG GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGTGAAG ACTTCAAGGT GGACAGTGAG 
AATGAGAGTG AGTCGGCTGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCAAGACGA AAGGCACCCT CGAAGAGGAG CCTTCGCTAC 
GCTGGCATGC CGCGAAGAAA GAGTGAGAGG GGGAGGAAGA GGGTGAGATA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG AGGAGGAGGG AGAGGAGAAG 
ACAAAATCGA AGAGAAGGAT TGAAAGTGAC AGTGAGGATA GTGATATTGG AAGGAGGAAA 
AAGAAGAAGA ATAGCATCGA TCTCCAGCGA TCTTCTCTGC GGAAGCTATC CACAAGGATC 
GACTATAAGA AAATGGCGGG GGAGAGCACA GAATCGGACG AAAATGATAG TAAGAGTGAA 
CGGGATCAGG ACTCAAACAA GGAGAATGAG GATGAATCTG GCGACGAAAG CAGTGATGAG 
GCTGTTAGCC AGAAAGTGAG GACTACCAAA CAACTCCCCC ATTCGCAAAT GGGACAATCT 
TACATTCCAA CCACTGTCAA TGGGGGTGCT AGTACAGGAG GCCTTCACGA CGGGGCTGCT 
TCACCGGGGG AGAGGGATGC TCATGGTGGC GCAATCGGAG GGACTGTAAA TGGAGAAGGC 
GAGGAGGAGG ACGACCTTTT ACAGGTCACC GACCTGATCG ACTATGTGAC ACAAGACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_783470.2
CDS	24..4946
Translation	MASVHKFPDFAVICSFLERYGEMLQLPDLTIPELQEAIEETKCDVPILREMIIKLMRRLIKNVNAEKWERHLVKISRYYSGMAAWEVDTLGYMQSKTETKLGLLKFLCDSQFDEPKSKFRLAVNEIEPETMRIQPIGRDKMGLVYWFQKDHDANIRVYREEQDDLDNSSWQMVSQDRDDLAGLLAVLKKGTNKLTSLELKTDDSESTTTSVTNDEDTELDIKEEIAKLRLKVSFRQETDSEGVKLETTMQAEVMKPEGTVKSETLAVKEEQMDTTDGQKEYIDDKKGVKEEPMESCDSKENLSKLKTEMETESSVQKDDCVASKLEEATKIKSEEGMVDREGLVDVKKEELGVKQEVPPCRRGSVIMKVGDGVKGYKPVSTPSGSEASVMKDDKEDGDAKAIAGESESKESNKSTDPSEKVLERAPGKGNSSKEEKEKSKDIVSDERTREDQDGEQGRPEGGSTSAVVPEEVSERAPRPASAPELTVTPPKTLAGEPMASSSTSSLIPSQASPRARTPTTSLFMRPWETAPAQPEKEMEEVEGEGTSVGVDSSRKDGQVPAQSKGGLEGKGSVAKQTQLQEDEGCKDNYSQSIPEVRKLVDVPKPKEDNPALEKSVQEKEATEKEEDRSARADPLENTNMSVTQEKGQETEVKDVVNKMEVSKDKSGPDRDAKVGKGVDLQEKDSKKSPAEAGVERSEGEGSRKRDGMKEKEKRKEAEEEDGKVATSEEDEDLLKGDGKVSGVEQHDESDGKTKCAEETDPKDQSDGKEKTGESKEEGSKVEEDVTADRPASKEMEELSEEGAKRETESTEKIDEEEKEDDGVEKDKTEAEEAVQPLRKGSRPLRPRRGKQQEEQPAETKKPKPAPKKRGRPKKKVEVSSQDEKTSQDEGFSQEDEEEKESKKKSISKELMEDEEEDEEDDEEEEEESDDDDNDDDYGRKKIQKKKGVRTAKKKRKKKRLRYTPKRKASQKAVNKIVEMEKDEDELPAKSSYAGKNKLKKADDSDWISEENDDTPCCKCGLYNHPRWILLCDKCDSGFHTACLRPPLMAIPDGNWFCPKCEHEELIVNLQAKLEDLDGDLKKKDRLARRDERKLKYKYTDVCAVNILPEDTTVVEKKKRSRRNDYEEEEDEEEEVEYEDQRNHRSHSSRSRRSQGSRSRRSQGSRRRRSRSSRHHRDRRSRPRRSQRDFVVPDFEDDYEVVSRRSQRSRKTIDYSFNDFDSAIKTAINDEVEEHKKAVDQIKSRWGVSRGKDMSTIMGDEVDEEEEQEGEETGEKRAEEVDGEAEEGKRRDRKGKKKRRLNDLDGSSEDENSEDFKVDSENESESAEDGSNFSEASEDSFLTSRRRASRRKAPSKRSLRYAGMPRRKSERGRKRVRYYSSAEDDEGSYYSGSSGGSYTGSDSEDDEGTSGGRRKAAAKVISYREYSDSDEGRLKKRKQAARTPTPSPSSSSEVEDYTQRKRKFRIDSDEEEEEEEEGEEKTKSKRRIESDSEDSDIGRRKKKKNSIDLQRSSLRKLSTRIDYKKMAGESTESDENDSKSERDQDSNKENEDESGDESSDEAVSQKVRTTKQLPHSQMGQSYIPTTVNGGASTGGLHDGAASPGERDAHGGAIGGTVNGEGEEEDDLLQVTDLIDYVTQDI

Target ORF information:

RefSeq Version	XM_778377.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_778377.4

ORF Insert Sequence:

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AACTGATAGT CAATCTGCAA GCAAAGCTGG AAGATCTCGA TGGTGACCTC 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACAG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCGAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGACGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGTCGAGGG AAGGACATGT CAACCATCAT GGGAGACGAA 
GTTGACGAGG AGGAGGAGCA GGAGGGGGAG GAGACGGGGG AGAAGAGAGC CGAGGAGGTG 
GACGGAGAAG CTGAAGAGGG GAAGAGGCGG GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGTGAAG ACTTCAAGGT GGACAGTGAG 
AATGAGAGTG AGTCGGCTGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCAAGACGA AAGGCACCCT CGAAGAGGAG CCTTCGCTAC 
GCTGGCATGC CGCGAAGAAA GAGTGAGAGG GGGAGGAAGA GGGTGAGATA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG AGGAGGAGGG AGAGGAGAAG 
ACAAAATCGA AGAGAAGGAT TGAAAGTGAC AGTGAGGATA GTGATATTGG AAGGAGGAAA 
AAGAAGAAGA ATAGCATCGA TCTCCAGCGA TCTTCTCTGC GGAAGCTATC CACAAGGATC 
GACTATAAGA AAATGGCGGG GGAGAGCACA GAATCGGACG AAAATGATAG TAAGAGTGAA 
CGGGATCAGG ACTCAAACAA GGAGAATGAG GATGAATCTG GCGACGAAAG CAGTGATGAG 
GCTGTTAGCC AGAAAGTGAG GACTACCAAA CAACTCCCCC ATTCGCAAAT GGGACAATCT 
TACATTCCAA CCACTGTCAA TGGGGGTGCT AGTACAGGAG GCCTTCACGA CGGGGCTGCT 
TCACCGGGGG AGAGGGATGC TCATGGTGGC GCAATCGGAG GGACTGTAAA TGGAGAAGGC 
GAGGAGGAGG ACGACCTTTT ACAGGTCACC GACCTGATCG ACTATGTGAC ACAAGACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC578189 ( XM_003725608.3 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_003725656.2 Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), mRNA.

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CloneID	OSd47991
Clone ID Related Accession (Same CDS sequence)	XM_003725608.3
Accession Version	XM_003725608.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	remodeling and spacing factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145597.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_003725608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AGTTGATAGT CAATCTGCAA GCAAAACTGG AAGATCTCGA TGGTGACCTG 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACGG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCAAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGATGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGCCGAGGG AAGGACATGT CCACCATCAT GGGAGACGAA 
GTCGACGAGG AGGAGGAGCA GGAGGGGGAG GAGGAGAAGG AGAAGAGAGC CGAGGAGGTG 
GATGGAGAAG CTGAAGAGGG GAAGAGGCGA GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGCGAAG ACTTCAAGGT GGACAGTGAG 
AACGAGAGTG AGTCGGCCGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCGAGACGA AAGGCACCCT CAAAGAGGAG CCTTCGCTAC 
GCCGGCATGC CGCGAAGGAA GAGTGAGAGG GGGAGGAAGA GGGTGAGGTA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG ACGAGGAGGA GGGAGGGGAG 
AAGACGAAAT CGAAGAGAAG GATTGAAAGT GACAGTGAGG ATAGTGATAT TGGAAGGAGG 
AAAAAGAAGA AGAATAGCAT CGATCTCCAG CGATCTTCTC TGCGGAAGCT ATCCACAAGG 
ATCGACTATA AGAAAATGGC GGGGGAGAGC ACAGAATCGG ACGAAAATGA TAGTAAGAGT 
GAGCGGGATC AGGACTCAAA CAAGGAGAAT GAGGACGAAT CTGGCGACGA AAGCAGTGAT 
GAGGCTGTTA GCCAGAAAGT GAGGACTACC AAACAACTCC CCCATTCGCA AATGGGACAA 
TCTTACATTC CAACCACTGT CAATGGGGGT GCTAGTACAG GAGGCCTTCA CGACGGGGCT 
GCTTCACCGG GGGAGAGGGA TGCTCATGGT GGCGCAATCG GAGGGACTGT AAATGGAGAA 
GGTGAGGAGG AGGACGACCT TTTACAGGTC ACCGACCTGA TCGACTATGT GACACAAGAC 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003725656.2
CDS	29..4954
Translation	MASVHKFPDFAVICSFLERYGEMLQLPDLTIPELQEAIEETKCDVPILREMIIKLMRRLIKNVNAEKWERHLVKISRYYSGMAAWEVDTLGYMQSKTETKLGLLKFLCDSQFDEPKSKFRLAVNEIEPETMRIQPIGRDKMGLVYWFQKDHDANIRVYREEQDDLDNSSWQMVSQDRDDLAGLLAVLKKGTNKLTSLELKTDDSESTTTSVTNDEDTELDIKEEIAKLRLKVSFRQETDSEGVKLETTMQAEVMKPEGTVKSETLAVKEEQMDTTDGQKEYIDDKKGVKEEPMESCDSKENLSKLKTEMETESSVQKDDCVASKLEEATKIKSEEGMVDREGLVDVKKEELGVKQEVPPCRRGSVIMKVGDGVKGYKPVSTPSGSEASVMKDDKEDGDAKAIAGESESKESNKSTDPSEKVLERAPGKGNSSKEEKEKSKDIVSDERTREDQDGEQGRPEGGSTSAVVPEEVSERAPRPASAPELTVTPPKTLAGEPMASSSTSSLIPSQASPRARTPTTSLFMRPWETAPAQPEKEMEEVEGEGTSVGVDSSRKDGQVPAQSKGGLEGKGSVAKQTQLQEDEGCKDNYSQSIPEVRKLVDVPKPKEDNPALEKSVQEKEATEKEEDRSARADPLENTNMSVTQEKGQETEVKDVVNKMEVSKDKSGPDRDAKVGKGVDLQEKDSKKSPAEAGVERSEGEGSRKRDGMKEKEKRKEAEEEDGKVATSEEDEDLLKGDGKVSGVEQHDESDGKTKCAEETDPKDQSDGKEKTGESKEEGSKVEEDVTADRPASKEMEELSEEGAKRETESTEKIDEEEKEDDGVEKDKTEAEEAVQPLRKGSRPLRPRRGKQQEEQPAETKKPKPAPKKRGRPKKKVEVSSQDEKTSQDEGFSQEDEEEKESKKKSISKELMEDEEEDEEDDEEEEEESDDDDNDDDYGRKKIQKKKGVRTAKKKRKKKRLRYTPKRKASQKAVNKIVEMEKDEDELPAKSSYAGKNKLKKADDSDWISEENDDTPCCKCGLYNHPRWILLCDKCDSGFHTACLRPPLMAIPDGNWFCPKCEHEELIVNLQAKLEDLDGDLKKKDRLARRDERKLKYKYTDVCAVNILPEDTTVVEKKKRSRRNDYEEEEDEEEEVEYEDQRNHRSHGSRSRRSQGSRSRRSQGSRRRRSRSSRHHQDRRSRPRRSQRDFVVPDFEDDYEVVSRRSQRSRKTIDYSFNDFDSAIKTAINDEVEEHKKAVDQIKSRWGVSRGKDMSTIMGDEVDEEEEQEGEEEKEKRAEEVDGEAEEGKRRDRKGKKKRRLNDLDGSSEDENSEDFKVDSENESESAEDGSNFSEASEDSFLTSRRRASRRKAPSKRSLRYAGMPRRKSERGRKRVRYYSSAEDDEGSYYSGSSGGSYTGSDSEDDEGTSGGRRKAAAKVISYREYSDSDEGRLKKRKQAARTPTPSPSSSSEVEDYTQRKRKFRIDSDEEEEEDEEEGGEKTKSKRRIESDSEDSDIGRRKKKKNSIDLQRSSLRKLSTRIDYKKMAGESTESDENDSKSERDQDSNKENEDESGDESSDEAVSQKVRTTKQLPHSQMGQSYIPTTVNGGASTGGLHDGAASPGERDAHGGAIGGTVNGEGEEEDDLLQVTDLIDYVTQDI

Target ORF information:

RefSeq Version	XM_003725608.3
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus remodeling and spacing factor 1 (LOC578189), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003725608.3

ORF Insert Sequence:

ATGGCGTCTG TACATAAGTT TCCCGATTTC GCCGTAATAT GCTCCTTTTT GGAGCGTTAC 
GGCGAAATGT TGCAGCTGCC CGACCTCACA ATCCCAGAAT TACAAGAAGC AATAGAAGAA 
ACAAAATGTG ATGTTCCAAT TTTGAGAGAG ATGATCATCA AATTGATGCG TCGTCTCATC 
AAAAATGTGA ACGCTGAAAA ATGGGAGAGA CATCTAGTCA AGATAAGCCG CTACTATTCA 
GGAATGGCAG CATGGGAGGT GGACACCCTA GGTTACATGC AGAGTAAAAC AGAAACAAAA 
TTAGGTCTCC TAAAGTTTCT ATGCGATAGC CAATTTGACG AGCCAAAGTC CAAGTTCAGG 
CTGGCGGTGA ATGAGATAGA GCCAGAGACC ATGAGGATAC AGCCAATCGG AAGGGATAAG 
ATGGGACTCG TCTACTGGTT TCAAAAGGAT CATGATGCTA ACATCAGAGT GTACAGAGAA 
GAACAAGATG ATTTAGACAA CTCTTCCTGG CAGATGGTCT CACAGGACCG AGATGATCTG 
GCAGGGCTTC TTGCTGTACT GAAGAAAGGC ACAAACAAAC TCACCTCGTT AGAACTCAAG 
ACAGATGACT CGGAGTCGAC CACGACGTCC GTCACCAACG ACGAGGACAC TGAGCTTGAC 
ATCAAGGAAG AAATTGCCAA GCTTCGCCTG AAGGTGTCAT TCAGGCAAGA AACAGACTCC 
GAAGGTGTGA AACTTGAAAC CACGATGCAG GCTGAAGTGA TGAAACCAGA AGGGACTGTG 
AAATCAGAGA CACTAGCAGT GAAAGAGGAG CAAATGGATA CCACCGATGG ACAGAAGGAA 
TATATTGATG ATAAGAAAGG AGTGAAAGAA GAACCAATGG AATCTTGCGA TTCAAAGGAG 
AATTTGTCTA AACTGAAAAC AGAAATGGAA ACAGAAAGTT CTGTTCAGAA AGATGACTGT 
GTTGCTTCGA AATTAGAAGA AGCTACCAAG ATCAAATCTG AAGAGGGGAT GGTTGACAGG 
GAAGGTTTGG TTGATGTGAA AAAAGAGGAG TTAGGAGTGA AACAGGAAGT ACCACCCTGC 
AGAAGAGGAT CAGTGATTAT GAAGGTAGGA GACGGTGTCA AAGGTTACAA GCCGGTTTCA 
ACTCCATCAG GGAGTGAAGC ATCAGTCATG AAGGATGATA AAGAGGATGG TGATGCGAAG 
GCCATTGCAG GAGAGAGCGA GTCTAAGGAA TCAAACAAAT CAACAGATCC TTCTGAGAAG 
GTGCTAGAAA GGGCACCTGG TAAAGGCAAT AGCAGCAAGG AGGAGAAGGA GAAATCAAAA 
GACATTGTAA GCGATGAGAG GACAAGAGAA GATCAAGATG GTGAACAGGG TAGACCTGAG 
GGCGGTTCAA CCTCTGCTGT GGTACCTGAG GAAGTCTCTG AAAGGGCACC CAGACCAGCG 
TCTGCACCTG AATTGACTGT GACACCGCCT AAGACCCTTG CAGGGGAACC GATGGCTTCC 
TCATCCACTT CATCTCTGAT CCCCAGCCAG GCATCACCAA GGGCCAGAAC GCCCACCACT 
AGCCTGTTCA TGAGACCGTG GGAGACTGCA CCAGCTCAGC CGGAGAAAGA AATGGAAGAG 
GTGGAGGGGG AGGGAACGTC AGTTGGAGTA GATTCTAGTC GGAAAGATGG TCAGGTGCCA 
GCACAGTCCA AAGGAGGATT AGAAGGGAAA GGTAGCGTGG CCAAGCAAAC GCAATTACAA 
GAAGATGAAG GATGCAAAGA CAATTACTCC CAGAGTATTC CCGAGGTTAG GAAATTGGTG 
GATGTCCCAA AACCCAAAGA GGACAACCCA GCATTAGAAA AGTCAGTTCA AGAAAAAGAG 
GCTACAGAAA AGGAGGAAGA CAGATCAGCC AGAGCAGATC CGTTGGAAAA TACAAACATG 
TCGGTTACAC AAGAGAAAGG ACAGGAAACA GAAGTCAAAG ATGTTGTGAA TAAGATGGAA 
GTGTCAAAGG ATAAATCAGG GCCCGATAGG GACGCTAAAG TAGGCAAAGG TGTTGACTTG 
CAAGAGAAGG ATTCCAAGAA GAGTCCTGCT GAAGCAGGTG TGGAGAGATC AGAAGGTGAA 
GGATCGAGGA AAAGGGATGG TATGAAAGAG AAGGAGAAGA GGAAAGAAGC AGAGGAGGAA 
GATGGTAAAG TTGCCACGAG TGAAGAAGAT GAGGATCTTT TGAAGGGAGA CGGTAAAGTG 
TCGGGTGTAG AGCAGCATGA TGAGAGTGAT GGAAAGACCA AGTGCGCAGA AGAAACTGAT 
CCAAAAGACC AATCAGACGG GAAGGAGAAG ACTGGAGAGT CGAAGGAGGA AGGTTCCAAA 
GTGGAGGAGG ACGTGACAGC AGACAGGCCC GCATCAAAGG AGATGGAAGA GCTGAGTGAA 
GAAGGAGCAA AGAGGGAAAC GGAGAGCACT GAAAAGATAG ATGAAGAAGA AAAGGAGGAT 
GACGGTGTTG AGAAGGACAA AACAGAAGCG GAGGAAGCCG TCCAGCCTCT GCGTAAAGGG 
TCTCGTCCTC TAAGACCAAG AAGAGGCAAA CAACAGGAGG AGCAACCGGC GGAGACGAAG 
AAACCTAAAC CAGCCCCAAA GAAAAGAGGA AGACCAAAAA AGAAAGTTGA AGTTTCAAGT 
CAAGATGAGA AGACAAGCCA GGATGAAGGT TTTAGCCAGG AGGATGAGGA AGAGAAGGAA 
AGCAAAAAGA AAAGCATTTC CAAAGAACTA ATGGAAGATG AGGAAGAAGA CGAAGAGGAC 
GATGAGGAAG AAGAAGAAGA AAGTGATGAC GATGACAACG ACGATGACTA CGGAAGGAAG 
AAAATTCAAA AAAAGAAAGG GGTGCGTACG GCGAAGAAGA AACGCAAGAA GAAAAGACTA 
CGGTACACTC CCAAGAGGAA AGCGTCGCAG AAAGCCGTCA ATAAGATAGT TGAAATGGAG 
AAAGATGAGG ACGAATTACC AGCGAAGAGC AGTTATGCAG GGAAGAACAA ACTGAAGAAG 
GCTGATGATA GTGACTGGAT CTCTGAAGAG AACGATGACA CCCCCTGCTG TAAATGTGGA 
CTGTACAACC ATCCCCGATG GATTCTCTTG TGTGATAAAT GCGACTCTGG TTTTCATACG 
GCTTGTCTAC GACCACCGCT CATGGCCATC CCAGACGGCA ACTGGTTCTG CCCAAAGTGT 
GAACATGAGG AGTTGATAGT CAATCTGCAA GCAAAACTGG AAGATCTCGA TGGTGACCTG 
AAAAAGAAGG ACAGACTAGC TAGAAGGGAT GAAAGAAAGC TGAAATATAA ATACACTGAT 
GTGTGTGCCG TCAATATATT GCCAGAGGAT ACAACGGTAG TAGAAAAGAA GAAGAGATCA 
CGACGTAATG ATTACGAAGA AGAAGAAGAC GAAGAGGAGG AGGTTGAATA CGAGGATCAA 
CGCAACCACA GATCCCACGG CTCGCGATCA CGTCGATCAC AGGGTTCGCG GTCGCGCCGA 
TCACAGGGCT CACGCCGTCG ACGATCGCGA AGCTCACGAC ATCATCAAGA TCGGCGAAGT 
AGACCTCGCC GATCTCAGAG AGACTTTGTG GTGCCCGATT TCGAAGATGA TTACGAGGTC 
GTGAGTCGGA GGTCGCAGAG GTCGCGTAAG ACTATTGATT ACAGCTTCAA TGACTTTGAC 
TCGGCTATCA AGACGGCCAT CAATGACGAG GTAGAGGAAC ATAAAAAAGC GGTGGACCAA 
ATCAAGTCCC GCTGGGGGGT GAGCCGAGGG AAGGACATGT CCACCATCAT GGGAGACGAA 
GTCGACGAGG AGGAGGAGCA GGAGGGGGAG GAGGAGAAGG AGAAGAGAGC CGAGGAGGTG 
GATGGAGAAG CTGAAGAGGG GAAGAGGCGA GACAGGAAAG GGAAGAAGAA GAGACGGCTC 
AACGATCTGG ACGGGTCTAG CGAGGACGAG AACAGCGAAG ACTTCAAGGT GGACAGTGAG 
AACGAGAGTG AGTCGGCCGA AGATGGCAGT AATTTCTCTG AAGCGAGTGA GGACAGCTTC 
TTGACATCGA GACGTCGCGC ATCGAGACGA AAGGCACCCT CAAAGAGGAG CCTTCGCTAC 
GCCGGCATGC CGCGAAGGAA GAGTGAGAGG GGGAGGAAGA GGGTGAGGTA CTACAGCTCA 
GCGGAAGATG ATGAAGGCAG CTATTACTCC GGAAGCTCAG GTGGCAGTTA CACCGGGAGT 
GATTCTGAGG ATGATGAGGG GACCTCTGGA GGAAGACGGA AAGCCGCGGC CAAGGTCATC 
TCCTACAGAG AATACTCCGA CAGCGACGAG GGGAGATTGA AGAAGAGGAA GCAGGCGGCC 
AGGACCCCGA CGCCTTCTCC GTCGTCATCT TCCGAGGTCG AGGATTACAC CCAACGAAAA 
AGGAAATTCC GCATCGATAG TGATGAGGAG GAGGAGGAGG ACGAGGAGGA GGGAGGGGAG 
AAGACGAAAT CGAAGAGAAG GATTGAAAGT GACAGTGAGG ATAGTGATAT TGGAAGGAGG 
AAAAAGAAGA AGAATAGCAT CGATCTCCAG CGATCTTCTC TGCGGAAGCT ATCCACAAGG 
ATCGACTATA AGAAAATGGC GGGGGAGAGC ACAGAATCGG ACGAAAATGA TAGTAAGAGT 
GAGCGGGATC AGGACTCAAA CAAGGAGAAT GAGGACGAAT CTGGCGACGA AAGCAGTGAT 
GAGGCTGTTA GCCAGAAAGT GAGGACTACC AAACAACTCC CCCATTCGCA AATGGGACAA 
TCTTACATTC CAACCACTGT CAATGGGGGT GCTAGTACAG GAGGCCTTCA CGACGGGGCT 
GCTTCACCGG GGGAGAGGGA TGCTCATGGT GGCGCAATCG GAGGGACTGT AAATGGAGAA 
GGTGAGGAGG AGGACGACCT TTTACAGGTC ACCGACCTGA TCGACTATGT GACACAAGAC 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC578189 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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