LOC105440495 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105440495
Entrez Gene ID	105440495
Full Name	uncharacterized LOC105440495
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011670713.2	XP_011669015.2	uncharacterized protein LOC105440495 isoform X3
XM_011670712.1	XP_011669014.1	uncharacterized protein LOC105440495 isoform X1
XM_011670712.2	XP_011669014.2	uncharacterized protein LOC105440495 isoform X1
XM_011670713.1	XP_011669015.1	uncharacterized protein LOC105440495 isoform X2
XM_030988681.1	XP_030844541.1	uncharacterized protein LOC105440495 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105440495 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105440495 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd54861	XM_011670712.2 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd54862	XM_030988681.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd54863	XM_011670713.2 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd36200	XM_011670712.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd36201	XM_011670713.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105440495 ( XM_011670712.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011669014.2 Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd54861
Clone ID Related Accession (Same CDS sequence)	XM_011670712.2
Accession Version	XM_011670712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC105440495 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145602.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011670712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGATTT ATTTGCTCTT GCAGCACCTC AATCTCAATC GGAAGAATGG 
AGCGAGTGGT CACATTGCTC CAGTTCATGT GGACACGGCA CCAAGACTAG ACATCGAGCA 
TGCACCGGAC CTAACACCAA CACCTGCCTC TACGGTGTCG AGTACGATGA TGTTAAATGC 
GCACTTCCGA AGTGCTCTGA CGAGTGGGAT GGAGCCGCAG TAGAGGTGGG AGAGGAGACG 
CCCTTGTCAC AATGGAGAGA TTGGTCTGGA TGGAGCGTGT GTTCGGTCTC CTGCGGGAGC 
GGTCTCAGTC ACCGGGATCG CTCCTGCCTC CAGGATCCAG ACAAGGGGCC TGGTTGCGGC 
ACTGCGCAGC GAGAGGAGAC GCGGACCTGC CGGCTAGACG AATGCAACAA TGTCGGAGGT 
GGTGGTTGGA AATCGGAAGA AGACGCTGCG AGGCGGACCG ACCACGGCCA ACATCATCAC 
CACACCAAAC GAATCAAATG CCATCTGGAT GAAAGTCGGG CTCATCTTCC CATGGAACTT 
TTTTGGACCA GCCCATCAGG CGAGAAAATC ACACATGCGA TGACACATGA GAAAAACTCT 
AAGTACCACA TAGAGGGAAC CACTTTGGTT GTGACCGAGG ATGAACCAAC TGAAGGGAGC 
TACCACTGCA TTGTCACTTA TGGTGGAAGC AGAGGCACAA ATGACAAAGG GAAAACGGAC 
CCGATGGGTG CTTGTCTTTC CCACCCGTGC CATCACAAGG GTGTGTGCCT GGACCAGCCC 
AGTGGACAAT ACAACGTCTC GTACCGCTGC ATGTGCCCGG CTGGATATGA GGGCATGACA 
TGCCACATGG CTCCTTATTT GAAGGGTGAC TTCATAGTCT TTTCTTTAAT GGGTTGGTTC 
ATCGTATCAG CCACTGTGAT AATAGTCGTC ATGTACTTGG GCTGCAGTAA AAAGATGAAG 
AAGCAGAACA TGAAACGAAA GTTGCAACTC GATGATGCTC CAGCAGAACA GCTACTAGCG 
AGGATTTTGC CTCAAATTGA AACTATGACC TATCCCGATA GCGAAACTGA CGAAGATACC 
AAGCCACTCT TGAGCTCCCG AACGCCTCAA TTTGGCAAGG AGCTATCAGC GGAGAAAAGT 
ATAACAACAC CAGAGTCAAC AGAGAAGCTT GAAGAATCGG GAATAGTGAG GTTTAAATCC 
TTCGTCAATA AGGTGACAGA AACGACTAGA AAAAAAGCTG AGGGATTCAC CTTCCCGACA 
CTTAAGCAGA TGAAGGAGAA TATAACAACA CCCGAGTCAA CAGAGGAGCT TGAACAATCG 
GGAAAGGTGA GGTTTAAATC CTTTGTCAAT AAGATGGCAG AAACGACTAG AAACAAGGAT 
CAGGGATTCA CTTTCCCGAC ATCTAAGCAG ATGATGTCGT TGGCTGACGA AATCAAAGCG 
CAGAGAAATA TGAATAATTC AAATACCCTG AATTCAAGCA CTAGCGAAGG ACAACATTCT 
GACTCAAACC CCACGCTTAG CAGTTCTTGT GAAAGTCTTT ATGAACGGGA CCACGCGGAA 
GGGAATGGTC GCTTGGTGAA CTATAACATG AATAATGGTC ACTACAGTTC TTGTGAAAGT 
CTTCCAGGAA AGAACAACGC TGAAGGATGT AGTCGGCTGG TGAACAACAA CATGAATAAC 
TGCTCCTTGG ACTACAGCAA TGTTTCATCA TGCGCTCCGT GCAGTGACCT CAATTCGTCA 
TCGATCGTCG GGAACGACGA TGACTCCCTC ATGGAGATGT CGACCGACGA TTTCCGTGAT 
TTTCAAGTCG CCAATGCTTT TTCGGTGTTC AAAGGGTCGC CATCGACGCG GGAAAAACAA 
GCAGTGATCA ACTCGTCGTT GGTGTATGGA AATTCAAACT CGACCCTTGT GTGTAATTCA 
AAGTCGTTGC TGGACGAGAT GCCACGTGCC TCTGAAAAAC ATCCCATGTT CCACTTTGAC 
CCCACAGCTA CAAATTTGAC GGGCCCATCT GACTGCAGTC CTGAGCGACA GAACTACGGT 
AATATCGGTT GTGGATTAGT TACAGCTACA AGTCCGCGGC GATGCGAACG ATGGCCAATG 
CGTACGCCCC CATCGAGAGG AAATGGAGTT CGAGTACGAC TTCCACCATC GCCGTCGCTC 
TCGGTCAGCC CGTCTCGGCT GTCGCTGCCT CCAATGGGGC AGAGAGGATC AAGGATGTTT 
GGGTCAACCG ATGAGCATGC CATCCTGGAA CAGTATCGTA AGCGTAGGAT GCCTCACCTT 
AAGCATGTCG GGTCCCCTGT GTCTTTTGAA ACGAGTCCGC AAGAATCGCT GGATGCTGCT 
AGGGACAGAA CTACATCTGG AACATCGTTG CCATGCTTCC ATCGTGGCCG CTCACATGGT 
ACTGACTCTA TTGCGGCATG TTTCAGAGAT AGATCGAGAA CAAGCCCTAG ATCGAAGAAG 
CATCAGGAGT ATCACGTTGC CACTGGGAAG TACAGTCCTA CCGATCTGAC ATCGATAGAT 
ACTCTGACAG ACGAGACACG TAGCAGACGA AGACTGCCTA CAAACAGGAG TTTGTTTAGA 
GAACGGGCGT CTGACAGTTC GGTACTGCTC CGTGATTCAA CGATGCATGC CAGTCGAGAT 
ATGCACCAAC CGTTCTTATT GGACGAAAGT TGTACAACGT CATCAGACTA TGTGAGCGTA 
GCCAATCAGT GTTCAGCAAG CACGAATGCT ATGACGTTTG GTTTCCCCGA ATACACTAAT 
ACAATGACTC GCATGTCTTC GTCTCCAAAT AGTAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011669014.2
CDS	92..4393
Translation	MTNRERMRLDRMEYQRLGSRTASINGRRDSGPFYRVSSTNRDDELRIPRRSDFETRQRESGLEVANNNEDRPDYASVDIPVWSRDGETISSMNLPKISQHEELGFRLFQEGTNSPNPTRTYSKDDPQSETPDDNIRSDYRESFAEYSEFVSADYDEIEDKIWDYHFNPDDGADAIYDTGVSVDTWGEAKSTKSKRDRSIIDAMNRRLVKQKDSMSASVTSERIMPELGMPQTEQAFRALQQDIINEERNEPESQEDITPRYSAESPDVRPNVPRINQGFRSSTDNERYGRSNYLRSAIGHKVARARASRSAKEESDFETSASEPIEYSYDPSSDLQEADEFTGDVDGRSLDAEKFRKLQEEILEEEEVGKSQRNSGGNSRDGLSDNRDDIKGIRNGPVGHEDRLGGSTLSRNDLPPNDHLEWRWGQNDDITLDESYLEHIGQAENNDDVTLIDESYLEHIGEAENNGAATADRPSAPEPELTSIYDDEAEGRREQAYDPVSGRNNDLFALAAPQSQSEEWSEWSHCSSSCGHGTKTRHRACTGPNTNTCLYGVEYDDVKCALPKCSDEWDGAAVEVGEETPLSQWRDWSGWSVCSVSCGSGLSHRDRSCLQDPDKGPGCGTAQREETRTCRLDECNNVGGGGWKSEEDAARRTDHGQHHHHTKRIKCHLDESRAHLPMELFWTSPSGEKITHAMTHEKNSKYHIEGTTLVVTEDEPTEGSYHCIVTYGGSRGTNDKGKTDPMGACLSHPCHHKGVCLDQPSGQYNVSYRCMCPAGYEGMTCHMAPYLKGDFIVFSLMGWFIVSATVIIVVMYLGCSKKMKKQNMKRKLQLDDAPAEQLLARILPQIETMTYPDSETDEDTKPLLSSRTPQFGKELSAEKSITTPESTEKLEESGIVRFKSFVNKVTETTRKKAEGFTFPTLKQMKENITTPESTEELEQSGKVRFKSFVNKMAETTRNKDQGFTFPTSKQMMSLADEIKAQRNMNNSNTLNSSTSEGQHSDSNPTLSSSCESLYERDHAEGNGRLVNYNMNNGHYSSCESLPGKNNAEGCSRLVNNNMNNCSLDYSNVSSCAPCSDLNSSSIVGNDDDSLMEMSTDDFRDFQVANAFSVFKGSPSTREKQAVINSSLVYGNSNSTLVCNSKSLLDEMPRASEKHPMFHFDPTATNLTGPSDCSPERQNYGNIGCGLVTATSPRRCERWPMRTPPSRGNGVRVRLPPSPSLSVSPSRLSLPPMGQRGSRMFGSTDEHAILEQYRKRRMPHLKHVGSPVSFETSPQESLDAARDRTTSGTSLPCFHRGRSHGTDSIAACFRDRSRTSPRSKKHQEYHVATGKYSPTDLTSIDTLTDETRSRRRLPTNRSLFRERASDSSVLLRDSTMHASRDMHQPFLLDESCTTSSDYVSVANQCSASTNAMTFGFPEYTNTMTRMSSSPNSNR

Target ORF information:

RefSeq Version	XM_011670712.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011670712.2

ORF Insert Sequence:

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGATTT ATTTGCTCTT GCAGCACCTC AATCTCAATC GGAAGAATGG 
AGCGAGTGGT CACATTGCTC CAGTTCATGT GGACACGGCA CCAAGACTAG ACATCGAGCA 
TGCACCGGAC CTAACACCAA CACCTGCCTC TACGGTGTCG AGTACGATGA TGTTAAATGC 
GCACTTCCGA AGTGCTCTGA CGAGTGGGAT GGAGCCGCAG TAGAGGTGGG AGAGGAGACG 
CCCTTGTCAC AATGGAGAGA TTGGTCTGGA TGGAGCGTGT GTTCGGTCTC CTGCGGGAGC 
GGTCTCAGTC ACCGGGATCG CTCCTGCCTC CAGGATCCAG ACAAGGGGCC TGGTTGCGGC 
ACTGCGCAGC GAGAGGAGAC GCGGACCTGC CGGCTAGACG AATGCAACAA TGTCGGAGGT 
GGTGGTTGGA AATCGGAAGA AGACGCTGCG AGGCGGACCG ACCACGGCCA ACATCATCAC 
CACACCAAAC GAATCAAATG CCATCTGGAT GAAAGTCGGG CTCATCTTCC CATGGAACTT 
TTTTGGACCA GCCCATCAGG CGAGAAAATC ACACATGCGA TGACACATGA GAAAAACTCT 
AAGTACCACA TAGAGGGAAC CACTTTGGTT GTGACCGAGG ATGAACCAAC TGAAGGGAGC 
TACCACTGCA TTGTCACTTA TGGTGGAAGC AGAGGCACAA ATGACAAAGG GAAAACGGAC 
CCGATGGGTG CTTGTCTTTC CCACCCGTGC CATCACAAGG GTGTGTGCCT GGACCAGCCC 
AGTGGACAAT ACAACGTCTC GTACCGCTGC ATGTGCCCGG CTGGATATGA GGGCATGACA 
TGCCACATGG CTCCTTATTT GAAGGGTGAC TTCATAGTCT TTTCTTTAAT GGGTTGGTTC 
ATCGTATCAG CCACTGTGAT AATAGTCGTC ATGTACTTGG GCTGCAGTAA AAAGATGAAG 
AAGCAGAACA TGAAACGAAA GTTGCAACTC GATGATGCTC CAGCAGAACA GCTACTAGCG 
AGGATTTTGC CTCAAATTGA AACTATGACC TATCCCGATA GCGAAACTGA CGAAGATACC 
AAGCCACTCT TGAGCTCCCG AACGCCTCAA TTTGGCAAGG AGCTATCAGC GGAGAAAAGT 
ATAACAACAC CAGAGTCAAC AGAGAAGCTT GAAGAATCGG GAATAGTGAG GTTTAAATCC 
TTCGTCAATA AGGTGACAGA AACGACTAGA AAAAAAGCTG AGGGATTCAC CTTCCCGACA 
CTTAAGCAGA TGAAGGAGAA TATAACAACA CCCGAGTCAA CAGAGGAGCT TGAACAATCG 
GGAAAGGTGA GGTTTAAATC CTTTGTCAAT AAGATGGCAG AAACGACTAG AAACAAGGAT 
CAGGGATTCA CTTTCCCGAC ATCTAAGCAG ATGATGTCGT TGGCTGACGA AATCAAAGCG 
CAGAGAAATA TGAATAATTC AAATACCCTG AATTCAAGCA CTAGCGAAGG ACAACATTCT 
GACTCAAACC CCACGCTTAG CAGTTCTTGT GAAAGTCTTT ATGAACGGGA CCACGCGGAA 
GGGAATGGTC GCTTGGTGAA CTATAACATG AATAATGGTC ACTACAGTTC TTGTGAAAGT 
CTTCCAGGAA AGAACAACGC TGAAGGATGT AGTCGGCTGG TGAACAACAA CATGAATAAC 
TGCTCCTTGG ACTACAGCAA TGTTTCATCA TGCGCTCCGT GCAGTGACCT CAATTCGTCA 
TCGATCGTCG GGAACGACGA TGACTCCCTC ATGGAGATGT CGACCGACGA TTTCCGTGAT 
TTTCAAGTCG CCAATGCTTT TTCGGTGTTC AAAGGGTCGC CATCGACGCG GGAAAAACAA 
GCAGTGATCA ACTCGTCGTT GGTGTATGGA AATTCAAACT CGACCCTTGT GTGTAATTCA 
AAGTCGTTGC TGGACGAGAT GCCACGTGCC TCTGAAAAAC ATCCCATGTT CCACTTTGAC 
CCCACAGCTA CAAATTTGAC GGGCCCATCT GACTGCAGTC CTGAGCGACA GAACTACGGT 
AATATCGGTT GTGGATTAGT TACAGCTACA AGTCCGCGGC GATGCGAACG ATGGCCAATG 
CGTACGCCCC CATCGAGAGG AAATGGAGTT CGAGTACGAC TTCCACCATC GCCGTCGCTC 
TCGGTCAGCC CGTCTCGGCT GTCGCTGCCT CCAATGGGGC AGAGAGGATC AAGGATGTTT 
GGGTCAACCG ATGAGCATGC CATCCTGGAA CAGTATCGTA AGCGTAGGAT GCCTCACCTT 
AAGCATGTCG GGTCCCCTGT GTCTTTTGAA ACGAGTCCGC AAGAATCGCT GGATGCTGCT 
AGGGACAGAA CTACATCTGG AACATCGTTG CCATGCTTCC ATCGTGGCCG CTCACATGGT 
ACTGACTCTA TTGCGGCATG TTTCAGAGAT AGATCGAGAA CAAGCCCTAG ATCGAAGAAG 
CATCAGGAGT ATCACGTTGC CACTGGGAAG TACAGTCCTA CCGATCTGAC ATCGATAGAT 
ACTCTGACAG ACGAGACACG TAGCAGACGA AGACTGCCTA CAAACAGGAG TTTGTTTAGA 
GAACGGGCGT CTGACAGTTC GGTACTGCTC CGTGATTCAA CGATGCATGC CAGTCGAGAT 
ATGCACCAAC CGTTCTTATT GGACGAAAGT TGTACAACGT CATCAGACTA TGTGAGCGTA 
GCCAATCAGT GTTCAGCAAG CACGAATGCT ATGACGTTTG GTTTCCCCGA ATACACTAAT 
ACAATGACTC GCATGTCTTC GTCTCCAAAT AGTAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105440495 ( XM_030988681.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030844541.1 Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd54862
Clone ID Related Accession (Same CDS sequence)	XM_030988681.1
Accession Version	XM_030988681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC105440495 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145602.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGATTT ATTTGCTCTT GCAGCACCTC AATCTCAATC GGAAGAATGG 
AGCGAGTGGT CACATTGCTC CAGTTCATGT GGACACGGCA CCAAGACTAG ACATCGAGCA 
TGCACCGGAC CTAACACCAA CACCTGCCTC TACGGTGTCG AGTACGATGA TGTTAAATGC 
GCACTTCCGA AGTGCTCTGA CGAGTGGGAT GGAGCCGCAG TAGAGGTGGG AGAGGAGACG 
CCCTTGTCAC AATGGAGAGA TTGGTCTGGA TGGAGCGTGT GTTCGGTCTC CTGCGGGAGC 
GGTCTCAGTC ACCGGGATCG CTCCTGCCTC CAGGATCCAG ACAAGGGGCC TGGTTGCGGC 
ACTGCGCAGC GAGAGGAGAC GCGGACCTGC CGGCTAGACG AATGCAACAA TGGTGGTGGT 
TGGAAATCGG AAGAAGACGC TGCGAGGCGG ACCGACCACG GCCAACATCA TCACCACACC 
AAACGAATCA AATGCCATCT GGATGAAAGT CGGGCTCATC TTCCCATGGA ACTTTTTTGG 
ACCAGCCCAT CAGGCGAGAA AATCACACAT GCGATGACAC ATGAGAAAAA CTCTAAGTAC 
CACATAGAGG GAACCACTTT GGTTGTGACC GAGGATGAAC CAACTGAAGG GAGCTACCAC 
TGCATTGTCA CTTATGGTGG AAGCAGAGGC ACAAATGACA AAGGGAAAAC GGACCCGATG 
GGTGCTTGTC TTTCCCACCC GTGCCATCAC AAGGGTGTGT GCCTGGACCA GCCCAGTGGA 
CAATACAACG TCTCGTACCG CTGCATGTGC CCGGCTGGAT ATGAGGGCAT GACATGCCAC 
ATGGCTCCTT ATTTGAAGGG TGACTTCATA GTCTTTTCTT TAATGGGTTG GTTCATCGTA 
TCAGCCACTG TGATAATAGT CGTCATGTAC TTGGGCTGCA GTAAAAAGAT GAAGAAGCAG 
AACATGAAAC GAAAGTTGCA ACTCGATGAT GCTCCAGCAG AACAGCTACT AGCGAGGATT 
TTGCCTCAAA TTGAAACTAT GACCTATCCC GATAGCGAAA CTGACGAAGA TACCAAGCCA 
CTCTTGAGCT CCCGAACGCC TCAATTTGGC AAGGAGCTAT CAGCGGAGAA AAGTATAACA 
ACACCAGAGT CAACAGAGAA GCTTGAAGAA TCGGGAATAG TGAGGTTTAA ATCCTTCGTC 
AATAAGGTGA CAGAAACGAC TAGAAAAAAA GCTGAGGGAT TCACCTTCCC GACACTTAAG 
CAGATGAAGG AGAATATAAC AACACCCGAG TCAACAGAGG AGCTTGAACA ATCGGGAAAG 
GTGAGGTTTA AATCCTTTGT CAATAAGATG GCAGAAACGA CTAGAAACAA GGATCAGGGA 
TTCACTTTCC CGACATCTAA GCAGATGATG TCGTTGGCTG ACGAAATCAA AGCGCAGAGA 
AATATGAATA ATTCAAATAC CCTGAATTCA AGCACTAGCG AAGGACAACA TTCTGACTCA 
AACCCCACGC TTAGCAGTTC TTGTGAAAGT CTTTATGAAC GGGACCACGC GGAAGGGAAT 
GGTCGCTTGG TGAACTATAA CATGAATAAT GGTCACTACA GTTCTTGTGA AAGTCTTCCA 
GGAAAGAACA ACGCTGAAGG ATGTAGTCGG CTGGTGAACA ACAACATGAA TAACTGCTCC 
TTGGACTACA GCAATGTTTC ATCATGCGCT CCGTGCAGTG ACCTCAATTC GTCATCGATC 
GTCGGGAACG ACGATGACTC CCTCATGGAG ATGTCGACCG ACGATTTCCG TGATTTTCAA 
GTCGCCAATG CTTTTTCGGT GTTCAAAGGG TCGCCATCGA CGCGGGAAAA ACAAGCAGTG 
ATCAACTCGT CGTTGGTGTA TGGAAATTCA AACTCGACCC TTGTGTGTAA TTCAAAGTCG 
TTGCTGGACG AGATGCCACG TGCCTCTGAA AAACATCCCA TGTTCCACTT TGACCCCACA 
GCTACAAATT TGACGGGCCC ATCTGACTGC AGTCCTGAGC GACAGAACTA CGGTAATATC 
GGTTGTGGAT TAGTTACAGC TACAAGTCCG CGGCGATGCG AACGATGGCC AATGCGTACG 
CCCCCATCGA GAGGAAATGG AGTTCGAGTA CGACTTCCAC CATCGCCGTC GCTCTCGGTC 
AGCCCGTCTC GGCTGTCGCT GCCTCCAATG GGGCAGAGAG GATCAAGGAT GTTTGGGTCA 
ACCGATGAGC ATGCCATCCT GGAACAGTAT CGTAAGCGTA GGATGCCTCA CCTTAAGCAT 
GTCGGGTCCC CTGTGTCTTT TGAAACGAGT CCGCAAGAAT CGCTGGATGC TGCTAGGGAC 
AGAACTACAT CTGGAACATC GTTGCCATGC TTCCATCGTG GCCGCTCACA TGGTACTGAC 
TCTATTGCGG CATGTTTCAG AGATAGATCG AGAACAAGCC CTAGATCGAA GAAGCATCAG 
GAGTATCACG TTGCCACTGG GAAGTACAGT CCTACCGATC TGACATCGAT AGATACTCTG 
ACAGACGAGA CACGTAGCAG ACGAAGACTG CCTACAAACA GGAGTTTGTT TAGAGAACGG 
GCGTCTGACA GTTCGGTACT GCTCCGTGAT TCAACGATGC ATGCCAGTCG AGATATGCAC 
CAACCGTTCT TATTGGACGA AAGTTGTACA ACGTCATCAG ACTATGTGAG CGTAGCCAAT 
CAGTGTTCAG CAAGCACGAA TGCTATGACG TTTGGTTTCC CCGAATACAC TAATACAATG 
ACTCGCATGT CTTCGTCTCC AAATAGTAAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030844541.1
CDS	92..4387
Translation	MTNRERMRLDRMEYQRLGSRTASINGRRDSGPFYRVSSTNRDDELRIPRRSDFETRQRESGLEVANNNEDRPDYASVDIPVWSRDGETISSMNLPKISQHEELGFRLFQEGTNSPNPTRTYSKDDPQSETPDDNIRSDYRESFAEYSEFVSADYDEIEDKIWDYHFNPDDGADAIYDTGVSVDTWGEAKSTKSKRDRSIIDAMNRRLVKQKDSMSASVTSERIMPELGMPQTEQAFRALQQDIINEERNEPESQEDITPRYSAESPDVRPNVPRINQGFRSSTDNERYGRSNYLRSAIGHKVARARASRSAKEESDFETSASEPIEYSYDPSSDLQEADEFTGDVDGRSLDAEKFRKLQEEILEEEEVGKSQRNSGGNSRDGLSDNRDDIKGIRNGPVGHEDRLGGSTLSRNDLPPNDHLEWRWGQNDDITLDESYLEHIGQAENNDDVTLIDESYLEHIGEAENNGAATADRPSAPEPELTSIYDDEAEGRREQAYDPVSGRNNDLFALAAPQSQSEEWSEWSHCSSSCGHGTKTRHRACTGPNTNTCLYGVEYDDVKCALPKCSDEWDGAAVEVGEETPLSQWRDWSGWSVCSVSCGSGLSHRDRSCLQDPDKGPGCGTAQREETRTCRLDECNNGGGWKSEEDAARRTDHGQHHHHTKRIKCHLDESRAHLPMELFWTSPSGEKITHAMTHEKNSKYHIEGTTLVVTEDEPTEGSYHCIVTYGGSRGTNDKGKTDPMGACLSHPCHHKGVCLDQPSGQYNVSYRCMCPAGYEGMTCHMAPYLKGDFIVFSLMGWFIVSATVIIVVMYLGCSKKMKKQNMKRKLQLDDAPAEQLLARILPQIETMTYPDSETDEDTKPLLSSRTPQFGKELSAEKSITTPESTEKLEESGIVRFKSFVNKVTETTRKKAEGFTFPTLKQMKENITTPESTEELEQSGKVRFKSFVNKMAETTRNKDQGFTFPTSKQMMSLADEIKAQRNMNNSNTLNSSTSEGQHSDSNPTLSSSCESLYERDHAEGNGRLVNYNMNNGHYSSCESLPGKNNAEGCSRLVNNNMNNCSLDYSNVSSCAPCSDLNSSSIVGNDDDSLMEMSTDDFRDFQVANAFSVFKGSPSTREKQAVINSSLVYGNSNSTLVCNSKSLLDEMPRASEKHPMFHFDPTATNLTGPSDCSPERQNYGNIGCGLVTATSPRRCERWPMRTPPSRGNGVRVRLPPSPSLSVSPSRLSLPPMGQRGSRMFGSTDEHAILEQYRKRRMPHLKHVGSPVSFETSPQESLDAARDRTTSGTSLPCFHRGRSHGTDSIAACFRDRSRTSPRSKKHQEYHVATGKYSPTDLTSIDTLTDETRSRRRLPTNRSLFRERASDSSVLLRDSTMHASRDMHQPFLLDESCTTSSDYVSVANQCSASTNAMTFGFPEYTNTMTRMSSSPNSNR

Target ORF information:

RefSeq Version	XM_030988681.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030988681.1

ORF Insert Sequence:

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGATTT ATTTGCTCTT GCAGCACCTC AATCTCAATC GGAAGAATGG 
AGCGAGTGGT CACATTGCTC CAGTTCATGT GGACACGGCA CCAAGACTAG ACATCGAGCA 
TGCACCGGAC CTAACACCAA CACCTGCCTC TACGGTGTCG AGTACGATGA TGTTAAATGC 
GCACTTCCGA AGTGCTCTGA CGAGTGGGAT GGAGCCGCAG TAGAGGTGGG AGAGGAGACG 
CCCTTGTCAC AATGGAGAGA TTGGTCTGGA TGGAGCGTGT GTTCGGTCTC CTGCGGGAGC 
GGTCTCAGTC ACCGGGATCG CTCCTGCCTC CAGGATCCAG ACAAGGGGCC TGGTTGCGGC 
ACTGCGCAGC GAGAGGAGAC GCGGACCTGC CGGCTAGACG AATGCAACAA TGGTGGTGGT 
TGGAAATCGG AAGAAGACGC TGCGAGGCGG ACCGACCACG GCCAACATCA TCACCACACC 
AAACGAATCA AATGCCATCT GGATGAAAGT CGGGCTCATC TTCCCATGGA ACTTTTTTGG 
ACCAGCCCAT CAGGCGAGAA AATCACACAT GCGATGACAC ATGAGAAAAA CTCTAAGTAC 
CACATAGAGG GAACCACTTT GGTTGTGACC GAGGATGAAC CAACTGAAGG GAGCTACCAC 
TGCATTGTCA CTTATGGTGG AAGCAGAGGC ACAAATGACA AAGGGAAAAC GGACCCGATG 
GGTGCTTGTC TTTCCCACCC GTGCCATCAC AAGGGTGTGT GCCTGGACCA GCCCAGTGGA 
CAATACAACG TCTCGTACCG CTGCATGTGC CCGGCTGGAT ATGAGGGCAT GACATGCCAC 
ATGGCTCCTT ATTTGAAGGG TGACTTCATA GTCTTTTCTT TAATGGGTTG GTTCATCGTA 
TCAGCCACTG TGATAATAGT CGTCATGTAC TTGGGCTGCA GTAAAAAGAT GAAGAAGCAG 
AACATGAAAC GAAAGTTGCA ACTCGATGAT GCTCCAGCAG AACAGCTACT AGCGAGGATT 
TTGCCTCAAA TTGAAACTAT GACCTATCCC GATAGCGAAA CTGACGAAGA TACCAAGCCA 
CTCTTGAGCT CCCGAACGCC TCAATTTGGC AAGGAGCTAT CAGCGGAGAA AAGTATAACA 
ACACCAGAGT CAACAGAGAA GCTTGAAGAA TCGGGAATAG TGAGGTTTAA ATCCTTCGTC 
AATAAGGTGA CAGAAACGAC TAGAAAAAAA GCTGAGGGAT TCACCTTCCC GACACTTAAG 
CAGATGAAGG AGAATATAAC AACACCCGAG TCAACAGAGG AGCTTGAACA ATCGGGAAAG 
GTGAGGTTTA AATCCTTTGT CAATAAGATG GCAGAAACGA CTAGAAACAA GGATCAGGGA 
TTCACTTTCC CGACATCTAA GCAGATGATG TCGTTGGCTG ACGAAATCAA AGCGCAGAGA 
AATATGAATA ATTCAAATAC CCTGAATTCA AGCACTAGCG AAGGACAACA TTCTGACTCA 
AACCCCACGC TTAGCAGTTC TTGTGAAAGT CTTTATGAAC GGGACCACGC GGAAGGGAAT 
GGTCGCTTGG TGAACTATAA CATGAATAAT GGTCACTACA GTTCTTGTGA AAGTCTTCCA 
GGAAAGAACA ACGCTGAAGG ATGTAGTCGG CTGGTGAACA ACAACATGAA TAACTGCTCC 
TTGGACTACA GCAATGTTTC ATCATGCGCT CCGTGCAGTG ACCTCAATTC GTCATCGATC 
GTCGGGAACG ACGATGACTC CCTCATGGAG ATGTCGACCG ACGATTTCCG TGATTTTCAA 
GTCGCCAATG CTTTTTCGGT GTTCAAAGGG TCGCCATCGA CGCGGGAAAA ACAAGCAGTG 
ATCAACTCGT CGTTGGTGTA TGGAAATTCA AACTCGACCC TTGTGTGTAA TTCAAAGTCG 
TTGCTGGACG AGATGCCACG TGCCTCTGAA AAACATCCCA TGTTCCACTT TGACCCCACA 
GCTACAAATT TGACGGGCCC ATCTGACTGC AGTCCTGAGC GACAGAACTA CGGTAATATC 
GGTTGTGGAT TAGTTACAGC TACAAGTCCG CGGCGATGCG AACGATGGCC AATGCGTACG 
CCCCCATCGA GAGGAAATGG AGTTCGAGTA CGACTTCCAC CATCGCCGTC GCTCTCGGTC 
AGCCCGTCTC GGCTGTCGCT GCCTCCAATG GGGCAGAGAG GATCAAGGAT GTTTGGGTCA 
ACCGATGAGC ATGCCATCCT GGAACAGTAT CGTAAGCGTA GGATGCCTCA CCTTAAGCAT 
GTCGGGTCCC CTGTGTCTTT TGAAACGAGT CCGCAAGAAT CGCTGGATGC TGCTAGGGAC 
AGAACTACAT CTGGAACATC GTTGCCATGC TTCCATCGTG GCCGCTCACA TGGTACTGAC 
TCTATTGCGG CATGTTTCAG AGATAGATCG AGAACAAGCC CTAGATCGAA GAAGCATCAG 
GAGTATCACG TTGCCACTGG GAAGTACAGT CCTACCGATC TGACATCGAT AGATACTCTG 
ACAGACGAGA CACGTAGCAG ACGAAGACTG CCTACAAACA GGAGTTTGTT TAGAGAACGG 
GCGTCTGACA GTTCGGTACT GCTCCGTGAT TCAACGATGC ATGCCAGTCG AGATATGCAC 
CAACCGTTCT TATTGGACGA AAGTTGTACA ACGTCATCAG ACTATGTGAG CGTAGCCAAT 
CAGTGTTCAG CAAGCACGAA TGCTATGACG TTTGGTTTCC CCGAATACAC TAATACAATG 
ACTCGCATGT CTTCGTCTCC AAATAGTAAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105440495 ( XM_011670713.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011669015.2 Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X3, mRNA.

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CloneID	OSd54863
Clone ID Related Accession (Same CDS sequence)	XM_011670713.2
Accession Version	XM_011670713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC105440495 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145602.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011670713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGCACC TCAATCTCAA TCGGAAGAAT GGAGCGAGTG GTCACATTGC 
TCCAGTTCAT GTGGACACGG CACCAAGACT AGACATCGAG CATGCACCGG ACCTAACACC 
AACACCTGCC TCTACGGTGT CGAGTACGAT GATGTTAAAT GCGCACTTCC GAAGTGCTCT 
GACGAGTGGG ATGGAGCCGC AGTAGAGGTG GGAGAGGAGA CGCCCTTGTC ACAATGGAGA 
GATTGGTCTG GATGGAGCGT GTGTTCGGTC TCCTGCGGGA GCGGTCTCAG TCACCGGGAT 
CGCTCCTGCC TCCAGGATCC AGACAAGGGG CCTGGTTGCG GCACTGCGCA GCGAGAGGAG 
ACGCGGACCT GCCGGCTAGA CGAATGCAAC AATGTCGGAG GTGGTGGTTG GAAATCGGAA 
GAAGACGCTG CGAGGCGGAC CGACCACGGC CAACATCATC ACCACACCAA ACGAATCAAA 
TGCCATCTGG ATGAAAGTCG GGCTCATCTT CCCATGGAAC TTTTTTGGAC CAGCCCATCA 
GGCGAGAAAA TCACACATGC GATGACACAT GAGAAAAACT CTAAGTACCA CATAGAGGGA 
ACCACTTTGG TTGTGACCGA GGATGAACCA ACTGAAGGGA GCTACCACTG CATTGTCACT 
TATGGTGGAA GCAGAGGCAC AAATGACAAA GGGAAAACGG ACCCGATGGG TGCTTGTCTT 
TCCCACCCGT GCCATCACAA GGGTGTGTGC CTGGACCAGC CCAGTGGACA ATACAACGTC 
TCGTACCGCT GCATGTGCCC GGCTGGATAT GAGGGCATGA CATGCCACAT GGCTCCTTAT 
TTGAAGGGTG ACTTCATAGT CTTTTCTTTA ATGGGTTGGT TCATCGTATC AGCCACTGTG 
ATAATAGTCG TCATGTACTT GGGCTGCAGT AAAAAGATGA AGAAGCAGAA CATGAAACGA 
AAGTTGCAAC TCGATGATGC TCCAGCAGAA CAGCTACTAG CGAGGATTTT GCCTCAAATT 
GAAACTATGA CCTATCCCGA TAGCGAAACT GACGAAGATA CCAAGCCACT CTTGAGCTCC 
CGAACGCCTC AATTTGGCAA GGAGCTATCA GCGGAGAAAA GTATAACAAC ACCAGAGTCA 
ACAGAGAAGC TTGAAGAATC GGGAATAGTG AGGTTTAAAT CCTTCGTCAA TAAGGTGACA 
GAAACGACTA GAAAAAAAGC TGAGGGATTC ACCTTCCCGA CACTTAAGCA GATGAAGGAG 
AATATAACAA CACCCGAGTC AACAGAGGAG CTTGAACAAT CGGGAAAGGT GAGGTTTAAA 
TCCTTTGTCA ATAAGATGGC AGAAACGACT AGAAACAAGG ATCAGGGATT CACTTTCCCG 
ACATCTAAGC AGATGATGTC GTTGGCTGAC GAAATCAAAG CGCAGAGAAA TATGAATAAT 
TCAAATACCC TGAATTCAAG CACTAGCGAA GGACAACATT CTGACTCAAA CCCCACGCTT 
AGCAGTTCTT GTGAAAGTCT TTATGAACGG GACCACGCGG AAGGGAATGG TCGCTTGGTG 
AACTATAACA TGAATAATGG TCACTACAGT TCTTGTGAAA GTCTTCCAGG AAAGAACAAC 
GCTGAAGGAT GTAGTCGGCT GGTGAACAAC AACATGAATA ACTGCTCCTT GGACTACAGC 
AATGTTTCAT CATGCGCTCC GTGCAGTGAC CTCAATTCGT CATCGATCGT CGGGAACGAC 
GATGACTCCC TCATGGAGAT GTCGACCGAC GATTTCCGTG ATTTTCAAGT CGCCAATGCT 
TTTTCGGTGT TCAAAGGGTC GCCATCGACG CGGGAAAAAC AAGCAGTGAT CAACTCGTCG 
TTGGTGTATG GAAATTCAAA CTCGACCCTT GTGTGTAATT CAAAGTCGTT GCTGGACGAG 
ATGCCACGTG CCTCTGAAAA ACATCCCATG TTCCACTTTG ACCCCACAGC TACAAATTTG 
ACGGGCCCAT CTGACTGCAG TCCTGAGCGA CAGAACTACG GTAATATCGG TTGTGGATTA 
GTTACAGCTA CAAGTCCGCG GCGATGCGAA CGATGGCCAA TGCGTACGCC CCCATCGAGA 
GGAAATGGAG TTCGAGTACG ACTTCCACCA TCGCCGTCGC TCTCGGTCAG CCCGTCTCGG 
CTGTCGCTGC CTCCAATGGG GCAGAGAGGA TCAAGGATGT TTGGGTCAAC CGATGAGCAT 
GCCATCCTGG AACAGTATCG TAAGCGTAGG ATGCCTCACC TTAAGCATGT CGGGTCCCCT 
GTGTCTTTTG AAACGAGTCC GCAAGAATCG CTGGATGCTG CTAGGGACAG AACTACATCT 
GGAACATCGT TGCCATGCTT CCATCGTGGC CGCTCACATG GTACTGACTC TATTGCGGCA 
TGTTTCAGAG ATAGATCGAG AACAAGCCCT AGATCGAAGA AGCATCAGGA GTATCACGTT 
GCCACTGGGA AGTACAGTCC TACCGATCTG ACATCGATAG ATACTCTGAC AGACGAGACA 
CGTAGCAGAC GAAGACTGCC TACAAACAGG AGTTTGTTTA GAGAACGGGC GTCTGACAGT 
TCGGTACTGC TCCGTGATTC AACGATGCAT GCCAGTCGAG ATATGCACCA ACCGTTCTTA 
TTGGACGAAA GTTGTACAAC GTCATCAGAC TATGTGAGCG TAGCCAATCA GTGTTCAGCA 
AGCACGAATG CTATGACGTT TGGTTTCCCC GAATACACTA ATACAATGAC TCGCATGTCT 
TCGTCTCCAA ATAGTAACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011669015.2
CDS	92..4375
Translation	MTNRERMRLDRMEYQRLGSRTASINGRRDSGPFYRVSSTNRDDELRIPRRSDFETRQRESGLEVANNNEDRPDYASVDIPVWSRDGETISSMNLPKISQHEELGFRLFQEGTNSPNPTRTYSKDDPQSETPDDNIRSDYRESFAEYSEFVSADYDEIEDKIWDYHFNPDDGADAIYDTGVSVDTWGEAKSTKSKRDRSIIDAMNRRLVKQKDSMSASVTSERIMPELGMPQTEQAFRALQQDIINEERNEPESQEDITPRYSAESPDVRPNVPRINQGFRSSTDNERYGRSNYLRSAIGHKVARARASRSAKEESDFETSASEPIEYSYDPSSDLQEADEFTGDVDGRSLDAEKFRKLQEEILEEEEVGKSQRNSGGNSRDGLSDNRDDIKGIRNGPVGHEDRLGGSTLSRNDLPPNDHLEWRWGQNDDITLDESYLEHIGQAENNDDVTLIDESYLEHIGEAENNGAATADRPSAPEPELTSIYDDEAEGRREQAYDPVSGRNNAPQSQSEEWSEWSHCSSSCGHGTKTRHRACTGPNTNTCLYGVEYDDVKCALPKCSDEWDGAAVEVGEETPLSQWRDWSGWSVCSVSCGSGLSHRDRSCLQDPDKGPGCGTAQREETRTCRLDECNNVGGGGWKSEEDAARRTDHGQHHHHTKRIKCHLDESRAHLPMELFWTSPSGEKITHAMTHEKNSKYHIEGTTLVVTEDEPTEGSYHCIVTYGGSRGTNDKGKTDPMGACLSHPCHHKGVCLDQPSGQYNVSYRCMCPAGYEGMTCHMAPYLKGDFIVFSLMGWFIVSATVIIVVMYLGCSKKMKKQNMKRKLQLDDAPAEQLLARILPQIETMTYPDSETDEDTKPLLSSRTPQFGKELSAEKSITTPESTEKLEESGIVRFKSFVNKVTETTRKKAEGFTFPTLKQMKENITTPESTEELEQSGKVRFKSFVNKMAETTRNKDQGFTFPTSKQMMSLADEIKAQRNMNNSNTLNSSTSEGQHSDSNPTLSSSCESLYERDHAEGNGRLVNYNMNNGHYSSCESLPGKNNAEGCSRLVNNNMNNCSLDYSNVSSCAPCSDLNSSSIVGNDDDSLMEMSTDDFRDFQVANAFSVFKGSPSTREKQAVINSSLVYGNSNSTLVCNSKSLLDEMPRASEKHPMFHFDPTATNLTGPSDCSPERQNYGNIGCGLVTATSPRRCERWPMRTPPSRGNGVRVRLPPSPSLSVSPSRLSLPPMGQRGSRMFGSTDEHAILEQYRKRRMPHLKHVGSPVSFETSPQESLDAARDRTTSGTSLPCFHRGRSHGTDSIAACFRDRSRTSPRSKKHQEYHVATGKYSPTDLTSIDTLTDETRSRRRLPTNRSLFRERASDSSVLLRDSTMHASRDMHQPFLLDESCTTSSDYVSVANQCSASTNAMTFGFPEYTNTMTRMSSSPNSNR

Target ORF information:

RefSeq Version	XM_011670713.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011670713.2

ORF Insert Sequence:

ATGACTAATC GTGAAAGAAT GAGGCTTGAC CGGATGGAGT ATCAACGTCT TGGGTCCCGA 
ACAGCATCGA TTAATGGTAG GCGTGACAGT GGCCCCTTTT ACAGAGTGTC TTCTACAAAC 
AGAGATGATG AACTTCGGAT ACCAAGGCGT TCGGACTTTG AGACTAGACA AAGGGAGAGC 
GGTCTTGAGG TGGCCAACAA CAACGAAGAT CGTCCGGATT ACGCTTCTGT GGACATCCCT 
GTGTGGTCCA GAGATGGCGA AACTATTTCC AGTATGAATT TACCCAAAAT ATCACAGCAT 
GAAGAACTTG GTTTTAGGTT ATTCCAAGAA GGAACGAACA GCCCAAACCC GACACGAACA 
TACTCCAAAG ATGATCCGCA GAGTGAAACA CCGGATGACA ACATCAGATC TGACTATAGG 
GAATCTTTTG CGGAATACAG TGAATTTGTG TCCGCAGACT ATGATGAAAT TGAAGACAAA 
ATTTGGGATT ATCACTTTAA TCCCGATGAT GGTGCAGATG CAATTTATGA TACCGGGGTT 
TCTGTGGATA CGTGGGGAGA AGCAAAATCA ACCAAGAGTA AACGAGATCG GAGCATTATA 
GACGCAATGA ATCGTCGTCT TGTAAAACAG AAGGACTCTA TGTCGGCATC AGTTACTTCA 
GAGAGGATTA TGCCTGAACT TGGTATGCCT CAAACTGAAC AAGCATTTCG AGCTCTGCAG 
CAGGATATCA TCAACGAAGA GCGAAACGAA CCAGAAAGCC AAGAAGACAT CACTCCGCGC 
TATTCTGCAG AGTCCCCAGA TGTCCGTCCG AACGTACCAC GGATCAATCA GGGTTTCCGG 
TCATCTACTG ATAACGAACG GTATGGTCGT TCAAATTATT TAAGAAGTGC TATAGGTCAC 
AAGGTTGCGC GGGCCAGAGC GTCGAGATCG GCGAAAGAAG AAAGTGACTT TGAAACGTCA 
GCATCGGAAC CAATAGAGTA CAGTTACGAC CCATCAAGTG ATCTGCAGGA AGCTGATGAA 
TTCACGGGGG ATGTAGACGG CAGGTCTTTA GACGCGGAAA AATTTCGTAA GCTGCAGGAG 
GAAATACTCG AGGAAGAGGA AGTAGGAAAG AGTCAACGAA ACAGTGGAGG AAATTCACGT 
GATGGTCTGT CTGATAATCG TGACGACATT AAAGGCATTC GAAATGGTCC GGTAGGGCAC 
GAAGACCGAC TCGGAGGCAG CACTCTTAGC AGAAATGACT TACCACCAAA TGATCATCTA 
GAATGGAGAT GGGGGCAAAA TGATGATATA ACTCTAGATG AGTCTTATCT AGAACACATT 
GGCCAGGCTG AAAACAATGA TGATGTAACT CTTATAGATG AATCTTATCT AGAACACATT 
GGCGAGGCTG AAAACAATGG TGCAGCGACT GCAGATAGGC CCTCTGCACC GGAGCCAGAA 
CTGACTTCTA TTTATGATGA TGAGGCAGAA GGGAGAAGGG AACAGGCTTA TGATCCGGTA 
TCAGGAAGAA ATAATGCACC TCAATCTCAA TCGGAAGAAT GGAGCGAGTG GTCACATTGC 
TCCAGTTCAT GTGGACACGG CACCAAGACT AGACATCGAG CATGCACCGG ACCTAACACC 
AACACCTGCC TCTACGGTGT CGAGTACGAT GATGTTAAAT GCGCACTTCC GAAGTGCTCT 
GACGAGTGGG ATGGAGCCGC AGTAGAGGTG GGAGAGGAGA CGCCCTTGTC ACAATGGAGA 
GATTGGTCTG GATGGAGCGT GTGTTCGGTC TCCTGCGGGA GCGGTCTCAG TCACCGGGAT 
CGCTCCTGCC TCCAGGATCC AGACAAGGGG CCTGGTTGCG GCACTGCGCA GCGAGAGGAG 
ACGCGGACCT GCCGGCTAGA CGAATGCAAC AATGTCGGAG GTGGTGGTTG GAAATCGGAA 
GAAGACGCTG CGAGGCGGAC CGACCACGGC CAACATCATC ACCACACCAA ACGAATCAAA 
TGCCATCTGG ATGAAAGTCG GGCTCATCTT CCCATGGAAC TTTTTTGGAC CAGCCCATCA 
GGCGAGAAAA TCACACATGC GATGACACAT GAGAAAAACT CTAAGTACCA CATAGAGGGA 
ACCACTTTGG TTGTGACCGA GGATGAACCA ACTGAAGGGA GCTACCACTG CATTGTCACT 
TATGGTGGAA GCAGAGGCAC AAATGACAAA GGGAAAACGG ACCCGATGGG TGCTTGTCTT 
TCCCACCCGT GCCATCACAA GGGTGTGTGC CTGGACCAGC CCAGTGGACA ATACAACGTC 
TCGTACCGCT GCATGTGCCC GGCTGGATAT GAGGGCATGA CATGCCACAT GGCTCCTTAT 
TTGAAGGGTG ACTTCATAGT CTTTTCTTTA ATGGGTTGGT TCATCGTATC AGCCACTGTG 
ATAATAGTCG TCATGTACTT GGGCTGCAGT AAAAAGATGA AGAAGCAGAA CATGAAACGA 
AAGTTGCAAC TCGATGATGC TCCAGCAGAA CAGCTACTAG CGAGGATTTT GCCTCAAATT 
GAAACTATGA CCTATCCCGA TAGCGAAACT GACGAAGATA CCAAGCCACT CTTGAGCTCC 
CGAACGCCTC AATTTGGCAA GGAGCTATCA GCGGAGAAAA GTATAACAAC ACCAGAGTCA 
ACAGAGAAGC TTGAAGAATC GGGAATAGTG AGGTTTAAAT CCTTCGTCAA TAAGGTGACA 
GAAACGACTA GAAAAAAAGC TGAGGGATTC ACCTTCCCGA CACTTAAGCA GATGAAGGAG 
AATATAACAA CACCCGAGTC AACAGAGGAG CTTGAACAAT CGGGAAAGGT GAGGTTTAAA 
TCCTTTGTCA ATAAGATGGC AGAAACGACT AGAAACAAGG ATCAGGGATT CACTTTCCCG 
ACATCTAAGC AGATGATGTC GTTGGCTGAC GAAATCAAAG CGCAGAGAAA TATGAATAAT 
TCAAATACCC TGAATTCAAG CACTAGCGAA GGACAACATT CTGACTCAAA CCCCACGCTT 
AGCAGTTCTT GTGAAAGTCT TTATGAACGG GACCACGCGG AAGGGAATGG TCGCTTGGTG 
AACTATAACA TGAATAATGG TCACTACAGT TCTTGTGAAA GTCTTCCAGG AAAGAACAAC 
GCTGAAGGAT GTAGTCGGCT GGTGAACAAC AACATGAATA ACTGCTCCTT GGACTACAGC 
AATGTTTCAT CATGCGCTCC GTGCAGTGAC CTCAATTCGT CATCGATCGT CGGGAACGAC 
GATGACTCCC TCATGGAGAT GTCGACCGAC GATTTCCGTG ATTTTCAAGT CGCCAATGCT 
TTTTCGGTGT TCAAAGGGTC GCCATCGACG CGGGAAAAAC AAGCAGTGAT CAACTCGTCG 
TTGGTGTATG GAAATTCAAA CTCGACCCTT GTGTGTAATT CAAAGTCGTT GCTGGACGAG 
ATGCCACGTG CCTCTGAAAA ACATCCCATG TTCCACTTTG ACCCCACAGC TACAAATTTG 
ACGGGCCCAT CTGACTGCAG TCCTGAGCGA CAGAACTACG GTAATATCGG TTGTGGATTA 
GTTACAGCTA CAAGTCCGCG GCGATGCGAA CGATGGCCAA TGCGTACGCC CCCATCGAGA 
GGAAATGGAG TTCGAGTACG ACTTCCACCA TCGCCGTCGC TCTCGGTCAG CCCGTCTCGG 
CTGTCGCTGC CTCCAATGGG GCAGAGAGGA TCAAGGATGT TTGGGTCAAC CGATGAGCAT 
GCCATCCTGG AACAGTATCG TAAGCGTAGG ATGCCTCACC TTAAGCATGT CGGGTCCCCT 
GTGTCTTTTG AAACGAGTCC GCAAGAATCG CTGGATGCTG CTAGGGACAG AACTACATCT 
GGAACATCGT TGCCATGCTT CCATCGTGGC CGCTCACATG GTACTGACTC TATTGCGGCA 
TGTTTCAGAG ATAGATCGAG AACAAGCCCT AGATCGAAGA AGCATCAGGA GTATCACGTT 
GCCACTGGGA AGTACAGTCC TACCGATCTG ACATCGATAG ATACTCTGAC AGACGAGACA 
CGTAGCAGAC GAAGACTGCC TACAAACAGG AGTTTGTTTA GAGAACGGGC GTCTGACAGT 
TCGGTACTGC TCCGTGATTC AACGATGCAT GCCAGTCGAG ATATGCACCA ACCGTTCTTA 
TTGGACGAAA GTTGTACAAC GTCATCAGAC TATGTGAGCG TAGCCAATCA GTGTTCAGCA 
AGCACGAATG CTATGACGTT TGGTTTCCCC GAATACACTA ATACAATGAC TCGCATGTCT 
TCGTCTCCAA ATAGTAACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105440495 ( XM_011670712.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011669014.1 Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.

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CloneID	OSd36200
Clone ID Related Accession (Same CDS sequence)	XM_011670712.1
Accession Version	XM_011670712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC105440495 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011978459.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAG TAAGAAAAGG CACTCGCGCG TTACGAAGGA AGCGCTTACC TGCCCATTCT 
TACATACCGA ATATTGAACA CCAAAGTACA GTGTCTCGTC CAGTTCGTGA CCTTCTGTTG 
TCTTCAAGGC AATCACCCAG GCAATTTCCT CAACGACGGC AGAACCGGCA AGTAAACAGT 
GCTCAAGCAC CTGCATATCA GTCTCCCCGT CAACAGAGTC GGGGTGCTGT GCGACAGCAG 
ACGGCTTCAG GAGCTCATCG AGGATATAGA CAACAAACAT CTGCTGGAAG AACTGCTCAC 
CAAACCAGTC TCCAACCACT GCAGTCCCAG GAACGTCCAC CCAACCAGCC GGCTAATCCT 
TACTATTGGC AAAGAAATTT CACCAACCCT AGTGAATATC CGCTACAAAA TCGCCCGCCA 
TCTATGGATG ACATCTACCG ACAGCGGATT CAGTCAGAGG TTAAAACGCT ACAACGTTTG 
CAGTCAAGTC GGCACCACAA AGAAAGAGAA CATCACGATA GAATTAATAA ACATGAGATC 
ATGAAGAAAC TACGGGACCT ACGCACAGAT CACCTAAGCA GGCTAAGTCG TCTCACCCCT 
GGAGAAAGTC ACTCACATGG ATCCTCATCG CCTCACCCGA GGAAACCGCC AAAGATAATC 
CAAAACTACT ACCCATTTGT ACCTCATGGA CGGGTGCAAG AAGACCATGG AGCGGCTGCT 
CCACAAAGGA GCACCAGACA AAAAACAACA AGTGAATCGA CAAAGATTAT CCCAAACTAC 
TACCCATTTG TACCCCATGG AGCGGCTGCT CCACATATTG GCACCCCGCA TAGAACAACA 
ACTGAATCGA CAAGCTTGGC TACGCCGACA ACAACGGCCA CGCCGACCAC AAAGGCTACG 
CCGACAACAA CGACTGCGAG CTCTCCTGTT GGTGCAAGAC CGATGACTAA CCGTGAAAGA 
ATGAGGCTTG ACCGGATGGA GTATCAACGT CTTGGGTCCC GAACAGCATC GATCAATGGT 
AGGCGTGACA GTGGCCCCTT TTACAGAGTG TCTTCTACAA ACAGAGATGA TGAACTTCGG 
ATACCAAGGC GTTCGGACTT TGAGACTAGA CAAAGGGAGA GCGGTCTTGA GGTGGCCAAC 
AACAACGAAG ATCGTCCGGA TTACGCTTCT GTGGACATCC CTGTGTGGTC CAGAGATGGC 
GAAACTATTT CCAGTATGAA TTTACCCAAA ATATCACAGC ATGAAGAACT TGGTTTTAGG 
TTATTACAAG AAGGAACGAA CAGCCCAAAC CCGACACGAA CATACTCCAA AGATGATCCG 
CAGAGTGAAA CACCGGATGA CAACATCAGA TCTGACTATA GGGAATCTTT TGCGGAATAC 
AGTGAATTTG TGTCCGCAGA CTATGATGAA ATTGAAGACA AAATTTGGGA TTATCACTTT 
AATCCCGATG ATGGTGCAGA TGCAATTTAT GATACCGGGG TTTCTGTGGA TACGTGGGGA 
GAAGCAAAAT CAACCAAGAG TAAACGAGAT CGGAGCATTA TAGACGCAAT GAATCGTCGT 
CTTGTAAAAC AGAAGGACTC TATGTCGGCA TCAGTTACTT CAGAGAGGAT TATGCCTGAA 
CTTGGTATGC CTCAAACTGA ACAAGCATTT CGAGCTCTGC AGCAGGATAT CATCAACGAA 
GAGCGAAACG AACCAGAAAG CCAAGAAGAC ATCACTCCGC GCTATTCTGC AAAGTCCCCA 
GATGTCCGTC CGAACGTACC ACAGATCAAT CAGGGTTTCC GGTCATCTAC TGATAACGAA 
CGGTATGGTC GTTCAAATTA TTTAAGAAGT GCTATAGGTC ACAAGGTTGC GCGGGCCAGA 
GCGTCGAGAT CGGCGAAAGA AGAAAGTGAC TTTGAGACGT CAGCATCGGA ACCAATAGAG 
TACAGTTACG ACCCATCAAG TGATCTGCAG GAAGCTGATG AATTCACGGG GGATGTAGAC 
GGCAGGTCTT TAGACGCGGA AAAATTTCGT AAGCTGCAGG AGGAAATACT CGAGGAAGAG 
GAAGTAGGAA AGAGTCAACG AAACAGTGGA GGAAATTCAC GTGATGGTCT GTCTGATAAT 
CGTGACGACA TTAAAGGCAT TCGAAACGGT CCGGTAGGGC ACGAAGACCG ACTCGGAGGC 
AGCACTCTAA GCAGAAATGA CTTACCACCA AATGATCATC TAGAATGGAG ATGGGGGCAA 
AATGATGATA TAACTCTAGA TGAGTCTTAT CTAGAACACA TTGGCCAGGC TGAAAACAAT 
GATGATGTAA CTCTTATAGA TGAATCTTAT CTAGAACACA TTGGCGAGGC TGAAAACAAT 
GGTGCAGCGA CTGCAGATAG GCCCTCTGCA CCGGAGCCAG AACTGACTTC TATTTATGAT 
GATGAGGCAG AAGGGAGAAT GGAACAGGCT TATGATCCGG TATCAGGAAG AAATAATGAT 
TTATTTGCTC TTGCAGCACC TCAATCTCAA TCGGAAGAAT GGAGCGAGTG GTCACATTGC 
TCCAGTTCAT GTGGACACGG CACCAAGACT AGACATCGAG CATGCACCGG ACCTAACACC 
AACACATGCC TCTACGGTGT CGAGTACGAT GATGTTAAAT GCGCACTTCC TAAGTGCTCT 
GACGAGTGGG ATGGAGCCGC AGTAGAGGTG GGAGAGGAGA CGCCCTTGTC ACAATGGAGA 
GATTGGTCTG GATGGAGCGT GTGTTCGGTC TCCTGCGGGA GCGGTCTCAG TCACCGGGAT 
CGCTCCTGCC TCCAGGATCC AGACAAGGGG CCTGGTTGCG GCACTGCGCA GCGAGAGGAG 
ACGCGGACCT GCCGGCTAGA CGAATGCAAC AATGTCGGAG GTGGTGGTTG GAAATCGGAA 
GAAGACGCTG CGAGGCGGAC CGACCACGGC CAACATCATC ACCACACCAA ACGAATCAAA 
TGCCATCTGG ATGAAAGTCG GGCTCATCTT CCTATGGAAC TTTTTTGGAC CAGCCCATCA 
GGCGAGAAAA TCACACATGC GATGACACAT GAGAAAAACT CTAAGTACCA CATAGAGGGA 
ACCACTTTGG TTGTGACCGA GGATGAACCA ACTGAAGGGA GCTACCACTG CATTGTCACT 
TATGGTGGAA GCAGAGGCAC AAATGACAAA GGGAAAACGG ACCCGATGGG TGCTTGTCTT 
TCCCACCCGT GCCATCACAA GGGTGTGTGC CTGGACCAGC CCAGTGGACA ATACAACGTC 
TCGTACCGCT GCATGTGCCC GGCTGGATAT GAGGGCATGA CATGCCACAT GGCTCCTTAT 
TTGAAGGGTG ACTTCATAGT CTTTTCTTTA ATGGGTTGGT TCATCGTATC AGCCACTGTG 
ATAATAGTCG TCATGTACTT GGGCTGCAGT AAAAAGATGA AGAAGCAGAA CATGAAACGA 
AAGTTGCAAC TCGATGATGC TCCAGCAGAA CAGCTACTAG CGAGGATTTT GCCTCAAATT 
GAAACTATGA CCTATCCCGA TAGCGAAACT GACGAAGATA CCAAGCCACT CTTGAGCTCC 
CGAACGCCTC AATTTGGCAA GGAGCTATCA GCGGAGAAAA CTATAACAAC ACCAGAGTCA 
ACAGAGAAGC TTGAAGAATC GGGAATAGTG AGGTTTAAAT CCTTCGTCAA TAAGGTGACA 
GAAACGACTA GAAAAAAAGC TGAGGGATTC ACCTTCCCGA CACTTAAGCA GATGAAGGAG 
AATATAACAA CACCCGAGTC AACAGAGGAG CTTGAACAAT CGGGAAAGGT GAGGTTTAAA 
TCCTTTGTCA ATAAGATGGC AGAAACGACT AGAAACAAGG ATCAGGGATT CACTTTCCCG 
ACATCTAAGC AGATGATGTC GTTGGCTGAC GAAATCAAAG CGCAGAGAAA TATGAATAAT 
TCAAATACCC TGAATTCAAG CACTAGCGAA GGACAATATT CTGACTCAAA CACCACGCTT 
AGCAGTTCTT GTGAAAGTCT TTATGAACGG GACCACGCGG AAGGGAATGG TCGCTTGGTG 
AACTATGACA TGAATAATGG TCACTACAGT TCTTGTGAAA GTCTTCCAGG AAAGAACAAC 
GCTGAAGGAT GTAGTCGGCT GGTGAACAAC AACATGAATA ACTGCTCCTT GGACTACAGC 
AATGTTTCAT CATGCGCTCC GTGCAGTGAC CTCAATTCGT CATCGATCGT CGGGAACGAC 
GATGACTCCC TCATGGAGAT GTCGACCGAC GATTTCCGTG ATTTTCAAGT CGCCAATGCT 
TTTTCGGTGT TCAAAGGGTC GCCATCGACG CGGGAAAAAC AAGCAGTGAT CAACTCGTCG 
TTGGTGTATG GAAATTCAAA CTCGACCCTT GTGTGTAATT CAAAGTCGTT GCTGGACCAG 
ATGCCAGGTG CCTCTGAAAA ACATCACATG TTCCACTTTG ACCCCACAGC TACAGATTTG 
ACGGGCCCAT CTGACTGCAG TCCTGAGCGA CAGAACTACG GTAATATCGG TTGTGGATTA 
GTTACAGCTA CAAGTCCGCG GCGGTGCGAA CGACGATGGC CAATGCGTAC GCCCCCATCG 
AGAGGAAATG GAGTTCGAGT ACGACTTCCA CCATCGCCGT CGCTCTCGGT CAGCCCGTCT 
CGGCTGTCGC TGCCTCCAAT GGGGCAGAGA GGATCAAGGA TGTTTGGGTC AACCGATGAG 
CATGCCATCC TGGAACAGTA TCGTAAGCGT AGGATGCCTC ACCTTAAGCA TGTCGGGTCC 
CCTGTGTCTT TTGAAACGAG TCCGCAAGAA TCGCTGGATG CTGCTAGGGA CAGAACTACA 
TCTGGAACAT CGTTGCCATG CTTCCATCGT GGCCGCTCAC ATGGTACTGA CTCTATTGCG 
GCATGTTTCA GAGATAGATC GAGAACAAGC CCTAGATCGA AGAAGCATCA GGAGTATCAC 
GTTGCCACTG GGAAGTACAG TCCTACCGAT CTGACATCGA TAGATACTCT GACAGACGAG 
ACACGTAGCA GACGAAGACT GCCTACAAAC AGGAGTTTGT TTAGAGAACG GGCGTCTGAC 
AGTTCGGTAC TGCTCCGTGA TTCAACGATG CATGCCAGTC GAGATATGCA CCAACCGTTC 
TTATTGGACG AAAGTTGTAC AACGTCATCA GACTATGTGA GCGTAGCCAA TCAGTGTTCA 
GCAAGCACGA ATGCTATGAC GTTTGGTTTC CCCGAATACA CTAATACAAT GACTCGCATG 
TCTTCGTCTC CAAATAGTAA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011669014.1
CDS	143..5389
Translation	MRAVRKGTRALRRKRLPAHSYIPNIEHQSTVSRPVRDLLLSSRQSPRQFPQRRQNRQVNSAQAPAYQSPRQQSRGAVRQQTASGAHRGYRQQTSAGRTAHQTSLQPLQSQERPPNQPANPYYWQRNFTNPSEYPLQNRPPSMDDIYRQRIQSEVKTLQRLQSSRHHKEREHHDRINKHEIMKKLRDLRTDHLSRLSRLTPGESHSHGSSSPHPRKPPKIIQNYYPFVPHGRVQEDHGAAAPQRSTRQKTTSESTKIIPNYYPFVPHGAAAPHIGTPHRTTTESTSLATPTTTATPTTKATPTTTTASSPVGARPMTNRERMRLDRMEYQRLGSRTASINGRRDSGPFYRVSSTNRDDELRIPRRSDFETRQRESGLEVANNNEDRPDYASVDIPVWSRDGETISSMNLPKISQHEELGFRLLQEGTNSPNPTRTYSKDDPQSETPDDNIRSDYRESFAEYSEFVSADYDEIEDKIWDYHFNPDDGADAIYDTGVSVDTWGEAKSTKSKRDRSIIDAMNRRLVKQKDSMSASVTSERIMPELGMPQTEQAFRALQQDIINEERNEPESQEDITPRYSAKSPDVRPNVPQINQGFRSSTDNERYGRSNYLRSAIGHKVARARASRSAKEESDFETSASEPIEYSYDPSSDLQEADEFTGDVDGRSLDAEKFRKLQEEILEEEEVGKSQRNSGGNSRDGLSDNRDDIKGIRNGPVGHEDRLGGSTLSRNDLPPNDHLEWRWGQNDDITLDESYLEHIGQAENNDDVTLIDESYLEHIGEAENNGAATADRPSAPEPELTSIYDDEAEGRMEQAYDPVSGRNNDLFALAAPQSQSEEWSEWSHCSSSCGHGTKTRHRACTGPNTNTCLYGVEYDDVKCALPKCSDEWDGAAVEVGEETPLSQWRDWSGWSVCSVSCGSGLSHRDRSCLQDPDKGPGCGTAQREETRTCRLDECNNVGGGGWKSEEDAARRTDHGQHHHHTKRIKCHLDESRAHLPMELFWTSPSGEKITHAMTHEKNSKYHIEGTTLVVTEDEPTEGSYHCIVTYGGSRGTNDKGKTDPMGACLSHPCHHKGVCLDQPSGQYNVSYRCMCPAGYEGMTCHMAPYLKGDFIVFSLMGWFIVSATVIIVVMYLGCSKKMKKQNMKRKLQLDDAPAEQLLARILPQIETMTYPDSETDEDTKPLLSSRTPQFGKELSAEKTITTPESTEKLEESGIVRFKSFVNKVTETTRKKAEGFTFPTLKQMKENITTPESTEELEQSGKVRFKSFVNKMAETTRNKDQGFTFPTSKQMMSLADEIKAQRNMNNSNTLNSSTSEGQYSDSNTTLSSSCESLYERDHAEGNGRLVNYDMNNGHYSSCESLPGKNNAEGCSRLVNNNMNNCSLDYSNVSSCAPCSDLNSSSIVGNDDDSLMEMSTDDFRDFQVANAFSVFKGSPSTREKQAVINSSLVYGNSNSTLVCNSKSLLDQMPGASEKHHMFHFDPTATDLTGPSDCSPERQNYGNIGCGLVTATSPRRCERRWPMRTPPSRGNGVRVRLPPSPSLSVSPSRLSLPPMGQRGSRMFGSTDEHAILEQYRKRRMPHLKHVGSPVSFETSPQESLDAARDRTTSGTSLPCFHRGRSHGTDSIAACFRDRSRTSPRSKKHQEYHVATGKYSPTDLTSIDTLTDETRSRRRLPTNRSLFRERASDSSVLLRDSTMHASRDMHQPFLLDESCTTSSDYVSVANQCSASTNAMTFGFPEYTNTMTRMSSSPNSNR

Target ORF information:

RefSeq Version	XM_011670712.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011670712.1

ORF Insert Sequence:

ATGAGAGCAG TAAGAAAAGG CACTCGCGCG TTACGAAGGA AGCGCTTACC TGCCCATTCT 
TACATACCGA ATATTGAACA CCAAAGTACA GTGTCTCGTC CAGTTCGTGA CCTTCTGTTG 
TCTTCAAGGC AATCACCCAG GCAATTTCCT CAACGACGGC AGAACCGGCA AGTAAACAGT 
GCTCAAGCAC CTGCATATCA GTCTCCCCGT CAACAGAGTC GGGGTGCTGT GCGACAGCAG 
ACGGCTTCAG GAGCTCATCG AGGATATAGA CAACAAACAT CTGCTGGAAG AACTGCTCAC 
CAAACCAGTC TCCAACCACT GCAGTCCCAG GAACGTCCAC CCAACCAGCC GGCTAATCCT 
TACTATTGGC AAAGAAATTT CACCAACCCT AGTGAATATC CGCTACAAAA TCGCCCGCCA 
TCTATGGATG ACATCTACCG ACAGCGGATT CAGTCAGAGG TTAAAACGCT ACAACGTTTG 
CAGTCAAGTC GGCACCACAA AGAAAGAGAA CATCACGATA GAATTAATAA ACATGAGATC 
ATGAAGAAAC TACGGGACCT ACGCACAGAT CACCTAAGCA GGCTAAGTCG TCTCACCCCT 
GGAGAAAGTC ACTCACATGG ATCCTCATCG CCTCACCCGA GGAAACCGCC AAAGATAATC 
CAAAACTACT ACCCATTTGT ACCTCATGGA CGGGTGCAAG AAGACCATGG AGCGGCTGCT 
CCACAAAGGA GCACCAGACA AAAAACAACA AGTGAATCGA CAAAGATTAT CCCAAACTAC 
TACCCATTTG TACCCCATGG AGCGGCTGCT CCACATATTG GCACCCCGCA TAGAACAACA 
ACTGAATCGA CAAGCTTGGC TACGCCGACA ACAACGGCCA CGCCGACCAC AAAGGCTACG 
CCGACAACAA CGACTGCGAG CTCTCCTGTT GGTGCAAGAC CGATGACTAA CCGTGAAAGA 
ATGAGGCTTG ACCGGATGGA GTATCAACGT CTTGGGTCCC GAACAGCATC GATCAATGGT 
AGGCGTGACA GTGGCCCCTT TTACAGAGTG TCTTCTACAA ACAGAGATGA TGAACTTCGG 
ATACCAAGGC GTTCGGACTT TGAGACTAGA CAAAGGGAGA GCGGTCTTGA GGTGGCCAAC 
AACAACGAAG ATCGTCCGGA TTACGCTTCT GTGGACATCC CTGTGTGGTC CAGAGATGGC 
GAAACTATTT CCAGTATGAA TTTACCCAAA ATATCACAGC ATGAAGAACT TGGTTTTAGG 
TTATTACAAG AAGGAACGAA CAGCCCAAAC CCGACACGAA CATACTCCAA AGATGATCCG 
CAGAGTGAAA CACCGGATGA CAACATCAGA TCTGACTATA GGGAATCTTT TGCGGAATAC 
AGTGAATTTG TGTCCGCAGA CTATGATGAA ATTGAAGACA AAATTTGGGA TTATCACTTT 
AATCCCGATG ATGGTGCAGA TGCAATTTAT GATACCGGGG TTTCTGTGGA TACGTGGGGA 
GAAGCAAAAT CAACCAAGAG TAAACGAGAT CGGAGCATTA TAGACGCAAT GAATCGTCGT 
CTTGTAAAAC AGAAGGACTC TATGTCGGCA TCAGTTACTT CAGAGAGGAT TATGCCTGAA 
CTTGGTATGC CTCAAACTGA ACAAGCATTT CGAGCTCTGC AGCAGGATAT CATCAACGAA 
GAGCGAAACG AACCAGAAAG CCAAGAAGAC ATCACTCCGC GCTATTCTGC AAAGTCCCCA 
GATGTCCGTC CGAACGTACC ACAGATCAAT CAGGGTTTCC GGTCATCTAC TGATAACGAA 
CGGTATGGTC GTTCAAATTA TTTAAGAAGT GCTATAGGTC ACAAGGTTGC GCGGGCCAGA 
GCGTCGAGAT CGGCGAAAGA AGAAAGTGAC TTTGAGACGT CAGCATCGGA ACCAATAGAG 
TACAGTTACG ACCCATCAAG TGATCTGCAG GAAGCTGATG AATTCACGGG GGATGTAGAC 
GGCAGGTCTT TAGACGCGGA AAAATTTCGT AAGCTGCAGG AGGAAATACT CGAGGAAGAG 
GAAGTAGGAA AGAGTCAACG AAACAGTGGA GGAAATTCAC GTGATGGTCT GTCTGATAAT 
CGTGACGACA TTAAAGGCAT TCGAAACGGT CCGGTAGGGC ACGAAGACCG ACTCGGAGGC 
AGCACTCTAA GCAGAAATGA CTTACCACCA AATGATCATC TAGAATGGAG ATGGGGGCAA 
AATGATGATA TAACTCTAGA TGAGTCTTAT CTAGAACACA TTGGCCAGGC TGAAAACAAT 
GATGATGTAA CTCTTATAGA TGAATCTTAT CTAGAACACA TTGGCGAGGC TGAAAACAAT 
GGTGCAGCGA CTGCAGATAG GCCCTCTGCA CCGGAGCCAG AACTGACTTC TATTTATGAT 
GATGAGGCAG AAGGGAGAAT GGAACAGGCT TATGATCCGG TATCAGGAAG AAATAATGAT 
TTATTTGCTC TTGCAGCACC TCAATCTCAA TCGGAAGAAT GGAGCGAGTG GTCACATTGC 
TCCAGTTCAT GTGGACACGG CACCAAGACT AGACATCGAG CATGCACCGG ACCTAACACC 
AACACATGCC TCTACGGTGT CGAGTACGAT GATGTTAAAT GCGCACTTCC TAAGTGCTCT 
GACGAGTGGG ATGGAGCCGC AGTAGAGGTG GGAGAGGAGA CGCCCTTGTC ACAATGGAGA 
GATTGGTCTG GATGGAGCGT GTGTTCGGTC TCCTGCGGGA GCGGTCTCAG TCACCGGGAT 
CGCTCCTGCC TCCAGGATCC AGACAAGGGG CCTGGTTGCG GCACTGCGCA GCGAGAGGAG 
ACGCGGACCT GCCGGCTAGA CGAATGCAAC AATGTCGGAG GTGGTGGTTG GAAATCGGAA 
GAAGACGCTG CGAGGCGGAC CGACCACGGC CAACATCATC ACCACACCAA ACGAATCAAA 
TGCCATCTGG ATGAAAGTCG GGCTCATCTT CCTATGGAAC TTTTTTGGAC CAGCCCATCA 
GGCGAGAAAA TCACACATGC GATGACACAT GAGAAAAACT CTAAGTACCA CATAGAGGGA 
ACCACTTTGG TTGTGACCGA GGATGAACCA ACTGAAGGGA GCTACCACTG CATTGTCACT 
TATGGTGGAA GCAGAGGCAC AAATGACAAA GGGAAAACGG ACCCGATGGG TGCTTGTCTT 
TCCCACCCGT GCCATCACAA GGGTGTGTGC CTGGACCAGC CCAGTGGACA ATACAACGTC 
TCGTACCGCT GCATGTGCCC GGCTGGATAT GAGGGCATGA CATGCCACAT GGCTCCTTAT 
TTGAAGGGTG ACTTCATAGT CTTTTCTTTA ATGGGTTGGT TCATCGTATC AGCCACTGTG 
ATAATAGTCG TCATGTACTT GGGCTGCAGT AAAAAGATGA AGAAGCAGAA CATGAAACGA 
AAGTTGCAAC TCGATGATGC TCCAGCAGAA CAGCTACTAG CGAGGATTTT GCCTCAAATT 
GAAACTATGA CCTATCCCGA TAGCGAAACT GACGAAGATA CCAAGCCACT CTTGAGCTCC 
CGAACGCCTC AATTTGGCAA GGAGCTATCA GCGGAGAAAA CTATAACAAC ACCAGAGTCA 
ACAGAGAAGC TTGAAGAATC GGGAATAGTG AGGTTTAAAT CCTTCGTCAA TAAGGTGACA 
GAAACGACTA GAAAAAAAGC TGAGGGATTC ACCTTCCCGA CACTTAAGCA GATGAAGGAG 
AATATAACAA CACCCGAGTC AACAGAGGAG CTTGAACAAT CGGGAAAGGT GAGGTTTAAA 
TCCTTTGTCA ATAAGATGGC AGAAACGACT AGAAACAAGG ATCAGGGATT CACTTTCCCG 
ACATCTAAGC AGATGATGTC GTTGGCTGAC GAAATCAAAG CGCAGAGAAA TATGAATAAT 
TCAAATACCC TGAATTCAAG CACTAGCGAA GGACAATATT CTGACTCAAA CACCACGCTT 
AGCAGTTCTT GTGAAAGTCT TTATGAACGG GACCACGCGG AAGGGAATGG TCGCTTGGTG 
AACTATGACA TGAATAATGG TCACTACAGT TCTTGTGAAA GTCTTCCAGG AAAGAACAAC 
GCTGAAGGAT GTAGTCGGCT GGTGAACAAC AACATGAATA ACTGCTCCTT GGACTACAGC 
AATGTTTCAT CATGCGCTCC GTGCAGTGAC CTCAATTCGT CATCGATCGT CGGGAACGAC 
GATGACTCCC TCATGGAGAT GTCGACCGAC GATTTCCGTG ATTTTCAAGT CGCCAATGCT 
TTTTCGGTGT TCAAAGGGTC GCCATCGACG CGGGAAAAAC AAGCAGTGAT CAACTCGTCG 
TTGGTGTATG GAAATTCAAA CTCGACCCTT GTGTGTAATT CAAAGTCGTT GCTGGACCAG 
ATGCCAGGTG CCTCTGAAAA ACATCACATG TTCCACTTTG ACCCCACAGC TACAGATTTG 
ACGGGCCCAT CTGACTGCAG TCCTGAGCGA CAGAACTACG GTAATATCGG TTGTGGATTA 
GTTACAGCTA CAAGTCCGCG GCGGTGCGAA CGACGATGGC CAATGCGTAC GCCCCCATCG 
AGAGGAAATG GAGTTCGAGT ACGACTTCCA CCATCGCCGT CGCTCTCGGT CAGCCCGTCT 
CGGCTGTCGC TGCCTCCAAT GGGGCAGAGA GGATCAAGGA TGTTTGGGTC AACCGATGAG 
CATGCCATCC TGGAACAGTA TCGTAAGCGT AGGATGCCTC ACCTTAAGCA TGTCGGGTCC 
CCTGTGTCTT TTGAAACGAG TCCGCAAGAA TCGCTGGATG CTGCTAGGGA CAGAACTACA 
TCTGGAACAT CGTTGCCATG CTTCCATCGT GGCCGCTCAC ATGGTACTGA CTCTATTGCG 
GCATGTTTCA GAGATAGATC GAGAACAAGC CCTAGATCGA AGAAGCATCA GGAGTATCAC 
GTTGCCACTG GGAAGTACAG TCCTACCGAT CTGACATCGA TAGATACTCT GACAGACGAG 
ACACGTAGCA GACGAAGACT GCCTACAAAC AGGAGTTTGT TTAGAGAACG GGCGTCTGAC 
AGTTCGGTAC TGCTCCGTGA TTCAACGATG CATGCCAGTC GAGATATGCA CCAACCGTTC 
TTATTGGACG AAAGTTGTAC AACGTCATCA GACTATGTGA GCGTAGCCAA TCAGTGTTCA 
GCAAGCACGA ATGCTATGAC GTTTGGTTTC CCCGAATACA CTAATACAAT GACTCGCATG 
TCTTCGTCTC CAAATAGTAA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105440495 ( XM_011670713.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011669015.1 Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.

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CloneID	OSd36201
Clone ID Related Accession (Same CDS sequence)	XM_011670713.1
Accession Version	XM_011670713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC105440495 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011978459.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAG TAAGAAAAGG CACTCGCGCG TTACGAAGGA AGCGCTTACC TGCCCATTCT 
TACATACCGA ATATTGAACA CCAAAGTACA GTGTCTCGTC CAGTTCGTGA CCTTCTGTTG 
TCTTCAAGGC AATCACCCAG GCAATTTCCT CAACGACGGC AGAACCGGCA AGTAAACAGT 
GCTCAAGCAC CTGCATATCA GTCTCCCCGT CAACAGAGTC GGGGTGCTGT GCGACAGCAG 
ACGGCTTCAG GAGCTCATCG AGGATATAGA CAACAAACAT CTGCTGGAAG AACTGCTCAC 
CAAACCAGTC TCCAACCACT GCAGTCCCAG GAACGTCCAC CCAACCAGCC GGCTAATCCT 
TACTATTGGC AAAGAAATTT CACCAACCCT AGTGAATATC CGCTACAAAA TCGCCCGCCA 
TCTATGGATG ACATCTACCG ACAGCGGATT CAGTCAGAGG TTAAAACGCT ACAACGTTTG 
CAGTCAAGTC GGCACCACAA AGAAAGAGAA CATCACGATA GAATTAATAA ACATGAGATC 
ATGAAGAAAC TACGGGACCT ACGCACAGAT CACCTAAGCA GGCTAAGTCG TCTCACCCCT 
GGAGAAAGTC ACTCACATGG ATCCTCATCG CCTCACCCGA GGAAACCGCC AAAGATAATC 
CAAAACTACT ACCCATTTGT ACCTCATGGA CGGGTGCAAG AAGACCATGG AGCGGCTGCT 
CCACAAAGGA GCACCAGACA AAAAACAACA AGTGAATCGA CAAAGATTAT CCCAAACTAC 
TACCCATTTG TACCCCATGG AGCGGCTGCT CCACATATTG GCACCCCGCA TAGAACAACA 
ACTGAATCGA CAAGCTTGGC TACGCCGACA ACAACGGCCA CGCCGACCAC AAAGGCTACG 
CCGACAACAA CGACTGCGAG CTCTCCTGTT GGTGCAAGAC CGATGACTAA CCGTGAAAGA 
ATGAGGCTTG ACCGGATGGA GTATCAACGT CTTGGGTCCC GAACAGCATC GATCAATGGT 
AGGCGTGACA GTGGCCCCTT TTACAGAGTG TCTTCTACAA ACAGAGATGA TGAACTTCGG 
ATACCAAGGC GTTCGGACTT TGAGACTAGA CAAAGGGAGA GCGGTCTTGA GGTGGCCAAC 
AACAACGAAG ATCGTCCGGA TTACGCTTCT GTGGACATCC CTGTGTGGTC CAGAGATGGC 
GAAACTATTT CCAGTATGAA TTTACCCAAA ATATCACAGC ATGAAGAACT TGGTTTTAGG 
TTATTACAAG AAGGAACGAA CAGCCCAAAC CCGACACGAA CATACTCCAA AGATGATCCG 
CAGAGTGAAA CACCGGATGA CAACATCAGA TCTGACTATA GGGAATCTTT TGCGGAATAC 
AGTGAATTTG TGTCCGCAGA CTATGATGAA ATTGAAGACA AAATTTGGGA TTATCACTTT 
AATCCCGATG ATGGTGCAGA TGCAATTTAT GATACCGGGG TTTCTGTGGA TACGTGGGGA 
GAAGCAAAAT CAACCAAGAG TAAACGAGAT CGGAGCATTA TAGACGCAAT GAATCGTCGT 
CTTGTAAAAC AGAAGGACTC TATGTCGGCA TCAGTTACTT CAGAGAGGAT TATGCCTGAA 
CTTGGTATGC CTCAAACTGA ACAAGCATTT CGAGCTCTGC AGCAGGATAT CATCAACGAA 
GAGCGAAACG AACCAGAAAG CCAAGAAGAC ATCACTCCGC GCTATTCTGC AAAGTCCCCA 
GATGTCCGTC CGAACGTACC ACAGATCAAT CAGGGTTTCC GGTCATCTAC TGATAACGAA 
CGGTATGGTC GTTCAAATTA TTTAAGAAGT GCTATAGGTC ACAAGGTTGC GCGGGCCAGA 
GCGTCGAGAT CGGCGAAAGA AGAAAGTGAC TTTGAGACGT CAGCATCGGA ACCAATAGAG 
TACAGTTACG ACCCATCAAG TGATCTGCAG GAAGCTGATG AATTCACGGG GGATGTAGAC 
GGCAGGTCTT TAGACGCGGA AAAATTTCGT AAGCTGCAGG AGGAAATACT CGAGGAAGAG 
GAAGTAGGAA AGAGTCAACG AAACAGTGGA GGAAATTCAC GTGATGGTCT GTCTGATAAT 
CGTGACGACA TTAAAGGCAT TCGAAACGGT CCGGTAGGGC ACGAAGACCG ACTCGGAGGC 
AGCACTCTAA GCAGAAATGA CTTACCACCA AATGATCATC TAGAATGGAG ATGGGGGCAA 
AATGATGATA TAACTCTAGA TGAGTCTTAT CTAGAACACA TTGGCCAGGC TGAAAACAAT 
GATGATGTAA CTCTTATAGA TGAATCTTAT CTAGAACACA TTGGCGAGGC TGAAAACAAT 
GGTGCAGCGA CTGCAGATAG GCCCTCTGCA CCGGAGCCAG AACTGACTTC TATTTATGAT 
GATGAGGCAG AAGGGAGAAT GGAACAGGCT TATGATCCGG TATCAGGAAG AAATAATGCA 
CCTCAATCTC AATCGGAAGA ATGGAGCGAG TGGTCACATT GCTCCAGTTC ATGTGGACAC 
GGCACCAAGA CTAGACATCG AGCATGCACC GGACCTAACA CCAACACATG CCTCTACGGT 
GTCGAGTACG ATGATGTTAA ATGCGCACTT CCTAAGTGCT CTGACGAGTG GGATGGAGCC 
GCAGTAGAGG TGGGAGAGGA GACGCCCTTG TCACAATGGA GAGATTGGTC TGGATGGAGC 
GTGTGTTCGG TCTCCTGCGG GAGCGGTCTC AGTCACCGGG ATCGCTCCTG CCTCCAGGAT 
CCAGACAAGG GGCCTGGTTG CGGCACTGCG CAGCGAGAGG AGACGCGGAC CTGCCGGCTA 
GACGAATGCA ACAATGTCGG AGGTGGTGGT TGGAAATCGG AAGAAGACGC TGCGAGGCGG 
ACCGACCACG GCCAACATCA TCACCACACC AAACGAATCA AATGCCATCT GGATGAAAGT 
CGGGCTCATC TTCCTATGGA ACTTTTTTGG ACCAGCCCAT CAGGCGAGAA AATCACACAT 
GCGATGACAC ATGAGAAAAA CTCTAAGTAC CACATAGAGG GAACCACTTT GGTTGTGACC 
GAGGATGAAC CAACTGAAGG GAGCTACCAC TGCATTGTCA CTTATGGTGG AAGCAGAGGC 
ACAAATGACA AAGGGAAAAC GGACCCGATG GGTGCTTGTC TTTCCCACCC GTGCCATCAC 
AAGGGTGTGT GCCTGGACCA GCCCAGTGGA CAATACAACG TCTCGTACCG CTGCATGTGC 
CCGGCTGGAT ATGAGGGCAT GACATGCCAC ATGGCTCCTT ATTTGAAGGG TGACTTCATA 
GTCTTTTCTT TAATGGGTTG GTTCATCGTA TCAGCCACTG TGATAATAGT CGTCATGTAC 
TTGGGCTGCA GTAAAAAGAT GAAGAAGCAG AACATGAAAC GAAAGTTGCA ACTCGATGAT 
GCTCCAGCAG AACAGCTACT AGCGAGGATT TTGCCTCAAA TTGAAACTAT GACCTATCCC 
GATAGCGAAA CTGACGAAGA TACCAAGCCA CTCTTGAGCT CCCGAACGCC TCAATTTGGC 
AAGGAGCTAT CAGCGGAGAA AACTATAACA ACACCAGAGT CAACAGAGAA GCTTGAAGAA 
TCGGGAATAG TGAGGTTTAA ATCCTTCGTC AATAAGGTGA CAGAAACGAC TAGAAAAAAA 
GCTGAGGGAT TCACCTTCCC GACACTTAAG CAGATGAAGG AGAATATAAC AACACCCGAG 
TCAACAGAGG AGCTTGAACA ATCGGGAAAG GTGAGGTTTA AATCCTTTGT CAATAAGATG 
GCAGAAACGA CTAGAAACAA GGATCAGGGA TTCACTTTCC CGACATCTAA GCAGATGATG 
TCGTTGGCTG ACGAAATCAA AGCGCAGAGA AATATGAATA ATTCAAATAC CCTGAATTCA 
AGCACTAGCG AAGGACAATA TTCTGACTCA AACACCACGC TTAGCAGTTC TTGTGAAAGT 
CTTTATGAAC GGGACCACGC GGAAGGGAAT GGTCGCTTGG TGAACTATGA CATGAATAAT 
GGTCACTACA GTTCTTGTGA AAGTCTTCCA GGAAAGAACA ACGCTGAAGG ATGTAGTCGG 
CTGGTGAACA ACAACATGAA TAACTGCTCC TTGGACTACA GCAATGTTTC ATCATGCGCT 
CCGTGCAGTG ACCTCAATTC GTCATCGATC GTCGGGAACG ACGATGACTC CCTCATGGAG 
ATGTCGACCG ACGATTTCCG TGATTTTCAA GTCGCCAATG CTTTTTCGGT GTTCAAAGGG 
TCGCCATCGA CGCGGGAAAA ACAAGCAGTG ATCAACTCGT CGTTGGTGTA TGGAAATTCA 
AACTCGACCC TTGTGTGTAA TTCAAAGTCG TTGCTGGACC AGATGCCAGG TGCCTCTGAA 
AAACATCACA TGTTCCACTT TGACCCCACA GCTACAGATT TGACGGGCCC ATCTGACTGC 
AGTCCTGAGC GACAGAACTA CGGTAATATC GGTTGTGGAT TAGTTACAGC TACAAGTCCG 
CGGCGGTGCG AACGACGATG GCCAATGCGT ACGCCCCCAT CGAGAGGAAA TGGAGTTCGA 
GTACGACTTC CACCATCGCC GTCGCTCTCG GTCAGCCCGT CTCGGCTGTC GCTGCCTCCA 
ATGGGGCAGA GAGGATCAAG GATGTTTGGG TCAACCGATG AGCATGCCAT CCTGGAACAG 
TATCGTAAGC GTAGGATGCC TCACCTTAAG CATGTCGGGT CCCCTGTGTC TTTTGAAACG 
AGTCCGCAAG AATCGCTGGA TGCTGCTAGG GACAGAACTA CATCTGGAAC ATCGTTGCCA 
TGCTTCCATC GTGGCCGCTC ACATGGTACT GACTCTATTG CGGCATGTTT CAGAGATAGA 
TCGAGAACAA GCCCTAGATC GAAGAAGCAT CAGGAGTATC ACGTTGCCAC TGGGAAGTAC 
AGTCCTACCG ATCTGACATC GATAGATACT CTGACAGACG AGACACGTAG CAGACGAAGA 
CTGCCTACAA ACAGGAGTTT GTTTAGAGAA CGGGCGTCTG ACAGTTCGGT ACTGCTCCGT 
GATTCAACGA TGCATGCCAG TCGAGATATG CACCAACCGT TCTTATTGGA CGAAAGTTGT 
ACAACGTCAT CAGACTATGT GAGCGTAGCC AATCAGTGTT CAGCAAGCAC GAATGCTATG 
ACGTTTGGTT TCCCCGAATA CACTAATACA ATGACTCGCA TGTCTTCGTC TCCAAATAGT 
AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011669015.1
CDS	143..5371
Translation	MRAVRKGTRALRRKRLPAHSYIPNIEHQSTVSRPVRDLLLSSRQSPRQFPQRRQNRQVNSAQAPAYQSPRQQSRGAVRQQTASGAHRGYRQQTSAGRTAHQTSLQPLQSQERPPNQPANPYYWQRNFTNPSEYPLQNRPPSMDDIYRQRIQSEVKTLQRLQSSRHHKEREHHDRINKHEIMKKLRDLRTDHLSRLSRLTPGESHSHGSSSPHPRKPPKIIQNYYPFVPHGRVQEDHGAAAPQRSTRQKTTSESTKIIPNYYPFVPHGAAAPHIGTPHRTTTESTSLATPTTTATPTTKATPTTTTASSPVGARPMTNRERMRLDRMEYQRLGSRTASINGRRDSGPFYRVSSTNRDDELRIPRRSDFETRQRESGLEVANNNEDRPDYASVDIPVWSRDGETISSMNLPKISQHEELGFRLLQEGTNSPNPTRTYSKDDPQSETPDDNIRSDYRESFAEYSEFVSADYDEIEDKIWDYHFNPDDGADAIYDTGVSVDTWGEAKSTKSKRDRSIIDAMNRRLVKQKDSMSASVTSERIMPELGMPQTEQAFRALQQDIINEERNEPESQEDITPRYSAKSPDVRPNVPQINQGFRSSTDNERYGRSNYLRSAIGHKVARARASRSAKEESDFETSASEPIEYSYDPSSDLQEADEFTGDVDGRSLDAEKFRKLQEEILEEEEVGKSQRNSGGNSRDGLSDNRDDIKGIRNGPVGHEDRLGGSTLSRNDLPPNDHLEWRWGQNDDITLDESYLEHIGQAENNDDVTLIDESYLEHIGEAENNGAATADRPSAPEPELTSIYDDEAEGRMEQAYDPVSGRNNAPQSQSEEWSEWSHCSSSCGHGTKTRHRACTGPNTNTCLYGVEYDDVKCALPKCSDEWDGAAVEVGEETPLSQWRDWSGWSVCSVSCGSGLSHRDRSCLQDPDKGPGCGTAQREETRTCRLDECNNVGGGGWKSEEDAARRTDHGQHHHHTKRIKCHLDESRAHLPMELFWTSPSGEKITHAMTHEKNSKYHIEGTTLVVTEDEPTEGSYHCIVTYGGSRGTNDKGKTDPMGACLSHPCHHKGVCLDQPSGQYNVSYRCMCPAGYEGMTCHMAPYLKGDFIVFSLMGWFIVSATVIIVVMYLGCSKKMKKQNMKRKLQLDDAPAEQLLARILPQIETMTYPDSETDEDTKPLLSSRTPQFGKELSAEKTITTPESTEKLEESGIVRFKSFVNKVTETTRKKAEGFTFPTLKQMKENITTPESTEELEQSGKVRFKSFVNKMAETTRNKDQGFTFPTSKQMMSLADEIKAQRNMNNSNTLNSSTSEGQYSDSNTTLSSSCESLYERDHAEGNGRLVNYDMNNGHYSSCESLPGKNNAEGCSRLVNNNMNNCSLDYSNVSSCAPCSDLNSSSIVGNDDDSLMEMSTDDFRDFQVANAFSVFKGSPSTREKQAVINSSLVYGNSNSTLVCNSKSLLDQMPGASEKHHMFHFDPTATDLTGPSDCSPERQNYGNIGCGLVTATSPRRCERRWPMRTPPSRGNGVRVRLPPSPSLSVSPSRLSLPPMGQRGSRMFGSTDEHAILEQYRKRRMPHLKHVGSPVSFETSPQESLDAARDRTTSGTSLPCFHRGRSHGTDSIAACFRDRSRTSPRSKKHQEYHVATGKYSPTDLTSIDTLTDETRSRRRLPTNRSLFRERASDSSVLLRDSTMHASRDMHQPFLLDESCTTSSDYVSVANQCSASTNAMTFGFPEYTNTMTRMSSSPNSNR

Target ORF information:

RefSeq Version	XM_011670713.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC105440495 (LOC105440495), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011670713.1

ORF Insert Sequence:

ATGAGAGCAG TAAGAAAAGG CACTCGCGCG TTACGAAGGA AGCGCTTACC TGCCCATTCT 
TACATACCGA ATATTGAACA CCAAAGTACA GTGTCTCGTC CAGTTCGTGA CCTTCTGTTG 
TCTTCAAGGC AATCACCCAG GCAATTTCCT CAACGACGGC AGAACCGGCA AGTAAACAGT 
GCTCAAGCAC CTGCATATCA GTCTCCCCGT CAACAGAGTC GGGGTGCTGT GCGACAGCAG 
ACGGCTTCAG GAGCTCATCG AGGATATAGA CAACAAACAT CTGCTGGAAG AACTGCTCAC 
CAAACCAGTC TCCAACCACT GCAGTCCCAG GAACGTCCAC CCAACCAGCC GGCTAATCCT 
TACTATTGGC AAAGAAATTT CACCAACCCT AGTGAATATC CGCTACAAAA TCGCCCGCCA 
TCTATGGATG ACATCTACCG ACAGCGGATT CAGTCAGAGG TTAAAACGCT ACAACGTTTG 
CAGTCAAGTC GGCACCACAA AGAAAGAGAA CATCACGATA GAATTAATAA ACATGAGATC 
ATGAAGAAAC TACGGGACCT ACGCACAGAT CACCTAAGCA GGCTAAGTCG TCTCACCCCT 
GGAGAAAGTC ACTCACATGG ATCCTCATCG CCTCACCCGA GGAAACCGCC AAAGATAATC 
CAAAACTACT ACCCATTTGT ACCTCATGGA CGGGTGCAAG AAGACCATGG AGCGGCTGCT 
CCACAAAGGA GCACCAGACA AAAAACAACA AGTGAATCGA CAAAGATTAT CCCAAACTAC 
TACCCATTTG TACCCCATGG AGCGGCTGCT CCACATATTG GCACCCCGCA TAGAACAACA 
ACTGAATCGA CAAGCTTGGC TACGCCGACA ACAACGGCCA CGCCGACCAC AAAGGCTACG 
CCGACAACAA CGACTGCGAG CTCTCCTGTT GGTGCAAGAC CGATGACTAA CCGTGAAAGA 
ATGAGGCTTG ACCGGATGGA GTATCAACGT CTTGGGTCCC GAACAGCATC GATCAATGGT 
AGGCGTGACA GTGGCCCCTT TTACAGAGTG TCTTCTACAA ACAGAGATGA TGAACTTCGG 
ATACCAAGGC GTTCGGACTT TGAGACTAGA CAAAGGGAGA GCGGTCTTGA GGTGGCCAAC 
AACAACGAAG ATCGTCCGGA TTACGCTTCT GTGGACATCC CTGTGTGGTC CAGAGATGGC 
GAAACTATTT CCAGTATGAA TTTACCCAAA ATATCACAGC ATGAAGAACT TGGTTTTAGG 
TTATTACAAG AAGGAACGAA CAGCCCAAAC CCGACACGAA CATACTCCAA AGATGATCCG 
CAGAGTGAAA CACCGGATGA CAACATCAGA TCTGACTATA GGGAATCTTT TGCGGAATAC 
AGTGAATTTG TGTCCGCAGA CTATGATGAA ATTGAAGACA AAATTTGGGA TTATCACTTT 
AATCCCGATG ATGGTGCAGA TGCAATTTAT GATACCGGGG TTTCTGTGGA TACGTGGGGA 
GAAGCAAAAT CAACCAAGAG TAAACGAGAT CGGAGCATTA TAGACGCAAT GAATCGTCGT 
CTTGTAAAAC AGAAGGACTC TATGTCGGCA TCAGTTACTT CAGAGAGGAT TATGCCTGAA 
CTTGGTATGC CTCAAACTGA ACAAGCATTT CGAGCTCTGC AGCAGGATAT CATCAACGAA 
GAGCGAAACG AACCAGAAAG CCAAGAAGAC ATCACTCCGC GCTATTCTGC AAAGTCCCCA 
GATGTCCGTC CGAACGTACC ACAGATCAAT CAGGGTTTCC GGTCATCTAC TGATAACGAA 
CGGTATGGTC GTTCAAATTA TTTAAGAAGT GCTATAGGTC ACAAGGTTGC GCGGGCCAGA 
GCGTCGAGAT CGGCGAAAGA AGAAAGTGAC TTTGAGACGT CAGCATCGGA ACCAATAGAG 
TACAGTTACG ACCCATCAAG TGATCTGCAG GAAGCTGATG AATTCACGGG GGATGTAGAC 
GGCAGGTCTT TAGACGCGGA AAAATTTCGT AAGCTGCAGG AGGAAATACT CGAGGAAGAG 
GAAGTAGGAA AGAGTCAACG AAACAGTGGA GGAAATTCAC GTGATGGTCT GTCTGATAAT 
CGTGACGACA TTAAAGGCAT TCGAAACGGT CCGGTAGGGC ACGAAGACCG ACTCGGAGGC 
AGCACTCTAA GCAGAAATGA CTTACCACCA AATGATCATC TAGAATGGAG ATGGGGGCAA 
AATGATGATA TAACTCTAGA TGAGTCTTAT CTAGAACACA TTGGCCAGGC TGAAAACAAT 
GATGATGTAA CTCTTATAGA TGAATCTTAT CTAGAACACA TTGGCGAGGC TGAAAACAAT 
GGTGCAGCGA CTGCAGATAG GCCCTCTGCA CCGGAGCCAG AACTGACTTC TATTTATGAT 
GATGAGGCAG AAGGGAGAAT GGAACAGGCT TATGATCCGG TATCAGGAAG AAATAATGCA 
CCTCAATCTC AATCGGAAGA ATGGAGCGAG TGGTCACATT GCTCCAGTTC ATGTGGACAC 
GGCACCAAGA CTAGACATCG AGCATGCACC GGACCTAACA CCAACACATG CCTCTACGGT 
GTCGAGTACG ATGATGTTAA ATGCGCACTT CCTAAGTGCT CTGACGAGTG GGATGGAGCC 
GCAGTAGAGG TGGGAGAGGA GACGCCCTTG TCACAATGGA GAGATTGGTC TGGATGGAGC 
GTGTGTTCGG TCTCCTGCGG GAGCGGTCTC AGTCACCGGG ATCGCTCCTG CCTCCAGGAT 
CCAGACAAGG GGCCTGGTTG CGGCACTGCG CAGCGAGAGG AGACGCGGAC CTGCCGGCTA 
GACGAATGCA ACAATGTCGG AGGTGGTGGT TGGAAATCGG AAGAAGACGC TGCGAGGCGG 
ACCGACCACG GCCAACATCA TCACCACACC AAACGAATCA AATGCCATCT GGATGAAAGT 
CGGGCTCATC TTCCTATGGA ACTTTTTTGG ACCAGCCCAT CAGGCGAGAA AATCACACAT 
GCGATGACAC ATGAGAAAAA CTCTAAGTAC CACATAGAGG GAACCACTTT GGTTGTGACC 
GAGGATGAAC CAACTGAAGG GAGCTACCAC TGCATTGTCA CTTATGGTGG AAGCAGAGGC 
ACAAATGACA AAGGGAAAAC GGACCCGATG GGTGCTTGTC TTTCCCACCC GTGCCATCAC 
AAGGGTGTGT GCCTGGACCA GCCCAGTGGA CAATACAACG TCTCGTACCG CTGCATGTGC 
CCGGCTGGAT ATGAGGGCAT GACATGCCAC ATGGCTCCTT ATTTGAAGGG TGACTTCATA 
GTCTTTTCTT TAATGGGTTG GTTCATCGTA TCAGCCACTG TGATAATAGT CGTCATGTAC 
TTGGGCTGCA GTAAAAAGAT GAAGAAGCAG AACATGAAAC GAAAGTTGCA ACTCGATGAT 
GCTCCAGCAG AACAGCTACT AGCGAGGATT TTGCCTCAAA TTGAAACTAT GACCTATCCC 
GATAGCGAAA CTGACGAAGA TACCAAGCCA CTCTTGAGCT CCCGAACGCC TCAATTTGGC 
AAGGAGCTAT CAGCGGAGAA AACTATAACA ACACCAGAGT CAACAGAGAA GCTTGAAGAA 
TCGGGAATAG TGAGGTTTAA ATCCTTCGTC AATAAGGTGA CAGAAACGAC TAGAAAAAAA 
GCTGAGGGAT TCACCTTCCC GACACTTAAG CAGATGAAGG AGAATATAAC AACACCCGAG 
TCAACAGAGG AGCTTGAACA ATCGGGAAAG GTGAGGTTTA AATCCTTTGT CAATAAGATG 
GCAGAAACGA CTAGAAACAA GGATCAGGGA TTCACTTTCC CGACATCTAA GCAGATGATG 
TCGTTGGCTG ACGAAATCAA AGCGCAGAGA AATATGAATA ATTCAAATAC CCTGAATTCA 
AGCACTAGCG AAGGACAATA TTCTGACTCA AACACCACGC TTAGCAGTTC TTGTGAAAGT 
CTTTATGAAC GGGACCACGC GGAAGGGAAT GGTCGCTTGG TGAACTATGA CATGAATAAT 
GGTCACTACA GTTCTTGTGA AAGTCTTCCA GGAAAGAACA ACGCTGAAGG ATGTAGTCGG 
CTGGTGAACA ACAACATGAA TAACTGCTCC TTGGACTACA GCAATGTTTC ATCATGCGCT 
CCGTGCAGTG ACCTCAATTC GTCATCGATC GTCGGGAACG ACGATGACTC CCTCATGGAG 
ATGTCGACCG ACGATTTCCG TGATTTTCAA GTCGCCAATG CTTTTTCGGT GTTCAAAGGG 
TCGCCATCGA CGCGGGAAAA ACAAGCAGTG ATCAACTCGT CGTTGGTGTA TGGAAATTCA 
AACTCGACCC TTGTGTGTAA TTCAAAGTCG TTGCTGGACC AGATGCCAGG TGCCTCTGAA 
AAACATCACA TGTTCCACTT TGACCCCACA GCTACAGATT TGACGGGCCC ATCTGACTGC 
AGTCCTGAGC GACAGAACTA CGGTAATATC GGTTGTGGAT TAGTTACAGC TACAAGTCCG 
CGGCGGTGCG AACGACGATG GCCAATGCGT ACGCCCCCAT CGAGAGGAAA TGGAGTTCGA 
GTACGACTTC CACCATCGCC GTCGCTCTCG GTCAGCCCGT CTCGGCTGTC GCTGCCTCCA 
ATGGGGCAGA GAGGATCAAG GATGTTTGGG TCAACCGATG AGCATGCCAT CCTGGAACAG 
TATCGTAAGC GTAGGATGCC TCACCTTAAG CATGTCGGGT CCCCTGTGTC TTTTGAAACG 
AGTCCGCAAG AATCGCTGGA TGCTGCTAGG GACAGAACTA CATCTGGAAC ATCGTTGCCA 
TGCTTCCATC GTGGCCGCTC ACATGGTACT GACTCTATTG CGGCATGTTT CAGAGATAGA 
TCGAGAACAA GCCCTAGATC GAAGAAGCAT CAGGAGTATC ACGTTGCCAC TGGGAAGTAC 
AGTCCTACCG ATCTGACATC GATAGATACT CTGACAGACG AGACACGTAG CAGACGAAGA 
CTGCCTACAA ACAGGAGTTT GTTTAGAGAA CGGGCGTCTG ACAGTTCGGT ACTGCTCCGT 
GATTCAACGA TGCATGCCAG TCGAGATATG CACCAACCGT TCTTATTGGA CGAAAGTTGT 
ACAACGTCAT CAGACTATGT GAGCGTAGCC AATCAGTGTT CAGCAAGCAC GAATGCTATG 
ACGTTTGGTT TCCCCGAATA CACTAATACA ATGACTCGCA TGTCTTCGTC TCCAAATAGT 
AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC105440495 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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