AHCTF1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	AHCTF1
Entrez Gene ID	105292577
Full Name	AT-hook containing transcription factor 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011360830.1	XP_011359132.1	protein ELYS isoform X1
XM_023530994.1	XP_023386762.1	protein ELYS isoform X1
XM_023530995.1	XP_023386763.1	protein ELYS isoform X2
XM_011360832.1	XP_011359134.1	protein ELYS isoform X2
XM_011360830.2	XP_011359132.1	protein ELYS isoform X1

Pathways from BioSystems
Gene Ontology

The following AHCTF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AHCTF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt47305	XM_023530994.1 Latest version!	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt47305	XM_011360830.2 Latest version!	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt47306	XM_011360832.1 Latest version!	Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt69723	XM_023530995.1 Latest version!	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OPt47305	XM_011360830.1	Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AHCTF1 ( XM_011360830.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011359132.1 Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt47305
Clone ID Related Accession (Same CDS sequence)	XM_011360830.1 , XM_023530994.1 , XM_011360830.2
Accession Version	XM_011360830.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	protein ELYS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888841.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011359132.1
CDS	385..7185
Translation	MHDLTAQVTSGLLHFPEVTVQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGVNEEPPIVLAVKEFSWQKRTGLLIGLEEAEGSILCLYDLGVSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRENVMREGRHLCFQLVGPSGTAVSTLSYVHRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPRNCCYLWAVQSTQDGEGDVLSLHLLQLAFGDRKCSASGQTLYEGLEYCEERYTLDLTGGMFPLRGQTNNTKLLGCQSIEKFRSHGDREEGTSEVLSPDTSVSVFTWQVNIYGQGKPSIYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFAFWSLDSVVSRTSPHYILDILVHERSLSRGVPPSYPPPEQFFYPSTYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGPSLNEAIPDGYNRCLVAGLLSPRLMDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCIKSWITEEQPNSAANLRFVLEWTWNKVIFTKEEFDRLCIPLFDGSRRFIDPQTIQSIQQCSLLLSNLNTVLSCFAAEAQDITERGAVNLSNKYMVSQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTGRRQKCEHLSRGKWNSDCLMIDGMVSQLGDRVEKLWKRDKGGTGKYPPTSLHALLDIYLLDNITEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHSDYESGLDLLFHPATTKPVSWQHSKIIQAFLSQGEHRQALRYIQTMKPAVSSDSDIVLLLTVLLFNRCMVEAWSLLRQHSSRSNIEELLKHTYDVCQEMGLMEDLLKLPFTDTEQECLVRFLQSSTSFQNQEFLLVHHLQRANYIPALKLNQTLKINAMNDRDPRLRERSVTRNSILDQYGKILPRVQRKLAIERAKPYCLSKSSVLREVSRPKPLSAVPKQAVIGTVLTRSTFISNVLSRIGEVWTSSEPKNNILTYNSPRVEVPSPIVYSFPDLELPEAFVGTPIGKASQRVSRLLDLVVHPVPQLSQCLGFIQQTTPTRSPLYLTSSSLPESPQLKGSRPNTSRASELNLLETPLVVKKAKTLAMSVASSGFAEFTPQSILRSGLRSTPLASPSVSPGRSLTPPLRLKETRISFMEEGMTTKWIAGAANDSKTKTFVTTPFYKCGVLAETEWPKNKDKTPSFYLNSPEMDHREMDVRSQDTHSQSLEKLDVSKENSNASSVSEQTTSEYQDAPSPGEFEMCVASRPENSSTELITNLTEEPEKDSDKDVFKFEVTPLDLEKQMGTLEDAETKALLAAEKVLSELNHSNPIQGVEASVCVPSIHEGKILTLKSQVPVSDKASVEACTSTVRAATADVLGDSGSSGLITSESPVISKHRLDQKIALNIKEVHELEAGALKASVDLPEEKPPISDDPPDTQEIHVIEHEKLEVQDSEQEARSLSFHEYPSGTLKLQYNFETIEQQFCDLPENKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASDMLAKAANIAAEEKHVYAGENDNCGHVAKLPSIMTSDQESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSETVEQSIHENIPLISQKVVSSTKLVKSTFKTVQETSTVTLNVSQSDDMVSSRTRTRARVQSQDVRSVSVQQEESADVATPETPGLSVKKKTRKKADISEASESAFSDVKEISQNRQIPQNSITPRRGKRKKEINQDTLESINSVERELQVTPGRELRRLRSSQLLEPLAKETSFRKEVKLSSVTKQTPKRIKKSVENQESVEIINDLKVSKVADPSRSTRKLRRAGLEASEDKGHGQDGKSSEQPPVQLSSKRVKKGDVSASDVIENSKLDSSQLTLQAEFDMPATPRKRGRPRKLHPSQDVGSEAVKEERSPKKSKALSMPRRSARNTPAKSESTGDGKPALEESVFVPNELATVMSSKKKITKKTETQGQKRSCHSIPEKGIDEEMTDEPNDQKEKMLATPLTRSSRRTRTRPSKAGLMPDLSEPKDESLLSPVPKVPRKEKGKKREASEQLKELVSDLSSQFISPPALRTRRKNISNTPILVDELEDDAQSETAGKRKVKGTRTEKSKQASRNIEEESSWSPPPVKIKLISPLASPVAGARHKPRKTAEVTGKALGRNRKKLSSFPKQILRRKML

Target ORF information:

RefSeq Version	XM_011360830.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360830.1

ORF Insert Sequence:

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHCTF1 ( XM_023530994.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023386762.1 Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

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CloneID	OPt47305
Clone ID Related Accession (Same CDS sequence)	XM_011360830.1 , XM_023530994.1 , XM_011360830.2
Accession Version	XM_023530994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	protein ELYS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888841.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023386762.1
CDS	27..6827
Translation	MHDLTAQVTSGLLHFPEVTVQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGVNEEPPIVLAVKEFSWQKRTGLLIGLEEAEGSILCLYDLGVSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRENVMREGRHLCFQLVGPSGTAVSTLSYVHRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPRNCCYLWAVQSTQDGEGDVLSLHLLQLAFGDRKCSASGQTLYEGLEYCEERYTLDLTGGMFPLRGQTNNTKLLGCQSIEKFRSHGDREEGTSEVLSPDTSVSVFTWQVNIYGQGKPSIYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFAFWSLDSVVSRTSPHYILDILVHERSLSRGVPPSYPPPEQFFYPSTYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGPSLNEAIPDGYNRCLVAGLLSPRLMDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCIKSWITEEQPNSAANLRFVLEWTWNKVIFTKEEFDRLCIPLFDGSRRFIDPQTIQSIQQCSLLLSNLNTVLSCFAAEAQDITERGAVNLSNKYMVSQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTGRRQKCEHLSRGKWNSDCLMIDGMVSQLGDRVEKLWKRDKGGTGKYPPTSLHALLDIYLLDNITEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHSDYESGLDLLFHPATTKPVSWQHSKIIQAFLSQGEHRQALRYIQTMKPAVSSDSDIVLLLTVLLFNRCMVEAWSLLRQHSSRSNIEELLKHTYDVCQEMGLMEDLLKLPFTDTEQECLVRFLQSSTSFQNQEFLLVHHLQRANYIPALKLNQTLKINAMNDRDPRLRERSVTRNSILDQYGKILPRVQRKLAIERAKPYCLSKSSVLREVSRPKPLSAVPKQAVIGTVLTRSTFISNVLSRIGEVWTSSEPKNNILTYNSPRVEVPSPIVYSFPDLELPEAFVGTPIGKASQRVSRLLDLVVHPVPQLSQCLGFIQQTTPTRSPLYLTSSSLPESPQLKGSRPNTSRASELNLLETPLVVKKAKTLAMSVASSGFAEFTPQSILRSGLRSTPLASPSVSPGRSLTPPLRLKETRISFMEEGMTTKWIAGAANDSKTKTFVTTPFYKCGVLAETEWPKNKDKTPSFYLNSPEMDHREMDVRSQDTHSQSLEKLDVSKENSNASSVSEQTTSEYQDAPSPGEFEMCVASRPENSSTELITNLTEEPEKDSDKDVFKFEVTPLDLEKQMGTLEDAETKALLAAEKVLSELNHSNPIQGVEASVCVPSIHEGKILTLKSQVPVSDKASVEACTSTVRAATADVLGDSGSSGLITSESPVISKHRLDQKIALNIKEVHELEAGALKASVDLPEEKPPISDDPPDTQEIHVIEHEKLEVQDSEQEARSLSFHEYPSGTLKLQYNFETIEQQFCDLPENKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASDMLAKAANIAAEEKHVYAGENDNCGHVAKLPSIMTSDQESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSETVEQSIHENIPLISQKVVSSTKLVKSTFKTVQETSTVTLNVSQSDDMVSSRTRTRARVQSQDVRSVSVQQEESADVATPETPGLSVKKKTRKKADISEASESAFSDVKEISQNRQIPQNSITPRRGKRKKEINQDTLESINSVERELQVTPGRELRRLRSSQLLEPLAKETSFRKEVKLSSVTKQTPKRIKKSVENQESVEIINDLKVSKVADPSRSTRKLRRAGLEASEDKGHGQDGKSSEQPPVQLSSKRVKKGDVSASDVIENSKLDSSQLTLQAEFDMPATPRKRGRPRKLHPSQDVGSEAVKEERSPKKSKALSMPRRSARNTPAKSESTGDGKPALEESVFVPNELATVMSSKKKITKKTETQGQKRSCHSIPEKGIDEEMTDEPNDQKEKMLATPLTRSSRRTRTRPSKAGLMPDLSEPKDESLLSPVPKVPRKEKGKKREASEQLKELVSDLSSQFISPPALRTRRKNISNTPILVDELEDDAQSETAGKRKVKGTRTEKSKQASRNIEEESSWSPPPVKIKLISPLASPVAGARHKPRKTAEVTGKALGRNRKKLSSFPKQILRRKML

Target ORF information:

RefSeq Version	XM_023530994.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023530994.1

ORF Insert Sequence:

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHCTF1 ( XM_011360830.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011359132.1 Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.

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CloneID	OPt47305
Clone ID Related Accession (Same CDS sequence)	XM_011360830.1 , XM_023530994.1 , XM_011360830.2
Accession Version	XM_011360830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	protein ELYS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888841.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011359132.1
CDS	398..7198
Translation	MHDLTAQVTSGLLHFPEVTVQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGVNEEPPIVLAVKEFSWQKRTGLLIGLEEAEGSILCLYDLGVSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRENVMREGRHLCFQLVGPSGTAVSTLSYVHRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPRNCCYLWAVQSTQDGEGDVLSLHLLQLAFGDRKCSASGQTLYEGLEYCEERYTLDLTGGMFPLRGQTNNTKLLGCQSIEKFRSHGDREEGTSEVLSPDTSVSVFTWQVNIYGQGKPSIYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFAFWSLDSVVSRTSPHYILDILVHERSLSRGVPPSYPPPEQFFYPSTYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGPSLNEAIPDGYNRCLVAGLLSPRLMDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCIKSWITEEQPNSAANLRFVLEWTWNKVIFTKEEFDRLCIPLFDGSRRFIDPQTIQSIQQCSLLLSNLNTVLSCFAAEAQDITERGAVNLSNKYMVSQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTGRRQKCEHLSRGKWNSDCLMIDGMVSQLGDRVEKLWKRDKGGTGKYPPTSLHALLDIYLLDNITEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHSDYESGLDLLFHPATTKPVSWQHSKIIQAFLSQGEHRQALRYIQTMKPAVSSDSDIVLLLTVLLFNRCMVEAWSLLRQHSSRSNIEELLKHTYDVCQEMGLMEDLLKLPFTDTEQECLVRFLQSSTSFQNQEFLLVHHLQRANYIPALKLNQTLKINAMNDRDPRLRERSVTRNSILDQYGKILPRVQRKLAIERAKPYCLSKSSVLREVSRPKPLSAVPKQAVIGTVLTRSTFISNVLSRIGEVWTSSEPKNNILTYNSPRVEVPSPIVYSFPDLELPEAFVGTPIGKASQRVSRLLDLVVHPVPQLSQCLGFIQQTTPTRSPLYLTSSSLPESPQLKGSRPNTSRASELNLLETPLVVKKAKTLAMSVASSGFAEFTPQSILRSGLRSTPLASPSVSPGRSLTPPLRLKETRISFMEEGMTTKWIAGAANDSKTKTFVTTPFYKCGVLAETEWPKNKDKTPSFYLNSPEMDHREMDVRSQDTHSQSLEKLDVSKENSNASSVSEQTTSEYQDAPSPGEFEMCVASRPENSSTELITNLTEEPEKDSDKDVFKFEVTPLDLEKQMGTLEDAETKALLAAEKVLSELNHSNPIQGVEASVCVPSIHEGKILTLKSQVPVSDKASVEACTSTVRAATADVLGDSGSSGLITSESPVISKHRLDQKIALNIKEVHELEAGALKASVDLPEEKPPISDDPPDTQEIHVIEHEKLEVQDSEQEARSLSFHEYPSGTLKLQYNFETIEQQFCDLPENKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASDMLAKAANIAAEEKHVYAGENDNCGHVAKLPSIMTSDQESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSETVEQSIHENIPLISQKVVSSTKLVKSTFKTVQETSTVTLNVSQSDDMVSSRTRTRARVQSQDVRSVSVQQEESADVATPETPGLSVKKKTRKKADISEASESAFSDVKEISQNRQIPQNSITPRRGKRKKEINQDTLESINSVERELQVTPGRELRRLRSSQLLEPLAKETSFRKEVKLSSVTKQTPKRIKKSVENQESVEIINDLKVSKVADPSRSTRKLRRAGLEASEDKGHGQDGKSSEQPPVQLSSKRVKKGDVSASDVIENSKLDSSQLTLQAEFDMPATPRKRGRPRKLHPSQDVGSEAVKEERSPKKSKALSMPRRSARNTPAKSESTGDGKPALEESVFVPNELATVMSSKKKITKKTETQGQKRSCHSIPEKGIDEEMTDEPNDQKEKMLATPLTRSSRRTRTRPSKAGLMPDLSEPKDESLLSPVPKVPRKEKGKKREASEQLKELVSDLSSQFISPPALRTRRKNISNTPILVDELEDDAQSETAGKRKVKGTRTEKSKQASRNIEEESSWSPPPVKIKLISPLASPVAGARHKPRKTAEVTGKALGRNRKKLSSFPKQILRRKML

Target ORF information:

RefSeq Version	XM_011360830.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360830.2

ORF Insert Sequence:

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAGAGA GGCTTCTGAA 
CAGCTGAAAG AATTGGTTTC AGATTTATCT TCTCAATTTA TTTCACCTCC CGCTCTAAGG 
ACCAGGCGGA AAAACATATC TAATACACCC ATTCTTGTAG ATGAACTGGA AGATGATGCT 
CAATCAGAAA CTGCGGGAAA GCGAAAAGTG AAAGGAACAA GGACTGAAAA ATCAAAGCAA 
GCAAGCAGAA ACATAGAAGA AGAAAGTTCT TGGTCACCTC CTCCAGTGAA AATTAAGCTG 
ATTTCACCCT TGGCAAGCCC GGTTGCTGGA GCAAGGCACA AGCCAAGGAA AACTGCAGAA 
GTGACAGGAA AAGCTCTTGG AAGGAACAGA AAGAAACTGT CTTCCTTTCC AAAACAGATT 
CTACGCAGAA AAATGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHCTF1 ( XM_011360832.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011359134.1 Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.

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CloneID	OPt47306
Clone ID Related Accession (Same CDS sequence)	XM_011360832.1
Accession Version	XM_011360832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	protein ELYS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888841.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAAGAG AGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011359134.1
CDS	386..6805
Translation	MHDLTAQVTSGLLHFPEVTVQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGVNEEPPIVLAVKEFSWQKRTGLLIGLEEAEGSILCLYDLGVSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRENVMREGRHLCFQLVGPSGTAVSTLSYVHRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPRNCCYLWAVQSTQDGEGDVLSLHLLQLAFGDRKCSASGQTLYEGLEYCEERYTLDLTGGMFPLRGQTNNTKLLGCQSIEKFRSHGDREEGTSEVLSPDTSVSVFTWQVNIYGQGKPSIYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFAFWSLDSVVSRTSPHYILDILVHERSLSRGVPPSYPPPEQFFYPSTYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGPSLNEAIPDGYNRCLVAGLLSPRLMDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCIKSWITEEQPNSAANLRFVLEWTWNKVIFTKEEFDRLCIPLFDGSRRFIDPQTIQSIQQCSLLLSNLNTVLSCFAAEAQDITERGAVNLSNKYMVSQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTGRRQKCEHLSRGKWNSDCLMIDGMVSQLGDRVEKLWKRDKGGTGKYPPTSLHALLDIYLLDNITEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHSDYESGLDLLFHPATTKPVSWQHSKIIQAFLSQGEHRQALRYIQTMKPAVSSDSDIVLLLTVLLFNRCMVEAWSLLRQHSSRSNIEELLKHTYDVCQEMGLMEDLLKLPFTDTEQECLVRFLQSSTSFQNQEFLLVHHLQRANYIPALKLNQTLKINAMNDRDPRLRERSVTRNSILDQYGKILPRVQRKLAIERAKPYCLSKSSVLREVSRPKPLSAVPKQAVIGTVLTRSTFISNVLSRIGEVWTSSEPKNNILTYNSPRVEVPSPIVYSFPDLELPEAFVGTPIGKASQRVSRLLDLVVHPVPQLSQCLGFIQQTTPTRSPLYLTSSSLPESPQLKGSRPNTSRASELNLLETPLVVKKAKTLAMSVASSGFAEFTPQSILRSGLRSTPLASPSVSPGRSLTPPLRLKETRISFMEEGMTTKWIAGAANDSKTKTFVTTPFYKCGVLAETEWPKNKDKTPSFYLNSPEMDHREMDVRSQDTHSQSLEKLDVSKENSNASSVSEQTTSEYQDAPSPGEFEMCVASRPENSSTELITNLTEEPEKDSDKDVFKFEVTPLDLEKQMGTLEDAETKALLAAEKVLSELNHSNPIQGVEASVCVPSIHEGKILTLKSQVPVSDKASVEACTSTVRAATADVLGDSGSSGLITSESPVISKHRLDQKIALNIKEVHELEAGALKASVDLPEEKPPISDDPPDTQEIHVIEHEKLEVQDSEQEARSLSFHEYPSGTLKLQYNFETIEQQFCDLPENKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASDMLAKAANIAAEEKHVYAGENDNCGHVAKLPSIMTSDQESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSETVEQSIHENIPLISQKVVSSTKLVKSTFKTVQETSTVTLNVSQSDDMVSSRTRTRARVQSQDVRSVSVQQEESADVATPETPGLSVKKKTRKKADISEASESAFSDVKEISQNRQIPQNSITPRRGKRKKEINQDTLESINSVERELQVTPGRELRRLRSSQLLEPLAKETSFRKEVKLSSVTKQTPKRIKKSVENQESVEIINDLKVSKVADPSRSTRKLRRAGLEASEDKGHGQDGKSSEQPPVQLSSKRVKKGDVSASDVIENSKLDSSQLTLQAEFDMPATPRKRGRPRKLHPSQDVGSEAVKEERSPKKSKALSMPRRSARNTPAKSESTGDGKPALEESVFVPNELATVMSSKKKITKKTETQGQKRSCHSIPEKGIDEEMTDEPNDQKEKMLATPLTRSSRRTRTRPSKAGLMPDLSEPKDESLLSPVPKVPRKEKGKKKRGF

Target ORF information:

RefSeq Version	XM_011360832.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus AT hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360832.1

ORF Insert Sequence:

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGGTA AAAAAAAGAG AGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHCTF1 ( XM_023530995.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023386763.1 Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X3, mRNA.

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CloneID	OPt69723
Clone ID Related Accession (Same CDS sequence)	XM_023530995.1
Accession Version	XM_023530995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	protein ELYS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888841.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023386763.1
CDS	27..6431
Translation	MHDLTAQVTSGLLHFPEVTVQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGVNEEPPIVLAVKEFSWQKRTGLLIGLEEAEGSILCLYDLGVSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRENVMREGRHLCFQLVGPSGTAVSTLSYVHRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPRNCCYLWAVQSTQDGEGDVLSLHLLQLAFGDRKCSASGQTLYEGLEYCEERYTLDLTGGMFPLRGQTNNTKLLGCQSIEKFRSHGDREEGTSEVLSPDTSVSVFTWQVNIYGQGKPSIYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFAFWSLDSVVSRTSPHYILDILVHERSLSRGVPPSYPPPEQFFYPSTYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGPSLNEAIPDGYNRCLVAGLLSPRLMDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCIKSWITEEQPNSAANLRFVLEWTWNKVIFTKEEFDRLCIPLFDGSRRFIDPQTIQSIQQCSLLLSNLNTVLSCFAAEAQDITERGAVNLSNKYMVSQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTGRRQKCEHLSRGKWNSDCLMIDGMVSQLGDRVEKLWKRDKGGTGKYPPTSLHALLDIYLLDNITEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHSDYESGLDLLFHPATTKPVSWQHSKIIQAFLSQGEHRQALRYIQTMKPAVSSDSDIVLLLTVLLFNRCMVEAWSLLRQHSSRSNIEELLKHTYDVCQEMGLMEDLLKLPFTDTEQECLVRFLQSSTSFQNQEFLLVHHLQRANYIPALKLNQTLKINAMNDRDPRLRERSVTRNSILDQYGKILPRVQRKLAIERAKPYCLSKSSVLREVSRPKPLSAVPKQAVIGTVLTRSTFISNVLSRIGEVWTSSEPKNNILTYNSPRVEVPSPIVYSFPDLELPEAFVGTPIGKASQRVSRLLDLVVHPVPQLSQCLGFIQQTTPTRSPLYLTSSSLPESPQLKGSRPNTSRASELNLLETPLVVKKAKTLAMSVASSGFAEFTPQSILRSGLRSTPLASPSVSPGRSLTPPLRLKETRISFMEEGMTTKWIAGAANDSKTKTFVTTPFYKCGVLAETEWPKNKDKTPSFYLNSPEMDHREMDVRSQDTHSQSLEKLDVSKENSNASSVSEQTTSEYQDAPSPGEFEMCVASRPENSSTELITNLTEEPEKDSDKDVFKFEVTPLDLEKQMGTLEDAETKALLAAEKVLSELNHSNPIQGVEASVCVPSIHEGKILTLKSQVPVSDKASVEACTSTVRAATADVLGDSGSSGLITSESPVISKHRLDQKIALNIKEVHELEAGALKASVDLPEEKPPISDDPPDTQEIHVIEHEKLEVQDSEQEARSLSFHEYPSGTLKLQYNFETIEQQFCDLPENKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASDMLAKAANIAAEEKHVYAGENDNCGHVAKLPSIMTSDQESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSETVEQSIHENIPLISQKVVSSTKLVKSTFKTVQETSTVTLNVSQSDDMVSSRTRTRARVQSQDVRSVSVQQEESADVATPETPGLSVKKKTRKKADISEASESAFSDVKEISQNRQIPQNSITPRRGKRKKEINQDTLESINSVERELQVTPGRELRRLRSSQLLEPLAKETSFRKEVKLSSVTKQTPKRIKKSVENQESVEIINDLKVSKVADPSRSTRKLRRAGLEASEDKGHGQDGKSSEQPPVQLSSKRVKKGDVSASDVIENSKLDSSQLTLQAEFDMPATPRKRGRPRKLHPSQDVGSEAVKEERSPKKSKALSMPRRSARNTPAKSESTGDGKPALEESVFVPNELATVMSSKKKITKKTETQGQKRSCHSIPEKGIDEEMTDEPNDQKEKMLATPLTRSSRRTRTRPSKAGLMPDLSEPKDESLLSPVPKVPRKEKDV

Target ORF information:

RefSeq Version	XM_023530995.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023530995.1

ORF Insert Sequence:

ATGCACGACT TAACAGCACA AGTGACTAGT GGTCTTCTGC ACTTCCCGGA GGTGACTGTT 
CAAGCCCTTG GAGAAGATGA AATAACATTG GAATCTGTGC TTCGTGGAAA GTTTGCTGCA 
GGGAAAAATG GACTTGCTTG CTTGGCTTGT GGTCCACAAC TTGAAGTAGT AAACTCTCTA 
ACAGGAGAGC GATTGTCCGC ATATAGGTTC AGTGGAGTGA ATGAAGAGCC ACCTATAGTC 
CTAGCTGTAA AAGAATTCTC TTGGCAGAAA AGGACTGGAT TGTTAATCGG ATTGGAGGAA 
GCCGAAGGAA GCATTCTCTG TCTTTATGAT CTTGGTGTAT CGAGAGTAGT TAAAGCAGTT 
GTTCTTCCTG GAAGGGTTAC AGCAATTGAA CCTATAATTA ATCATGGAGG AGCCAGTGCA 
AGCACTCAGC ATTTACACCC AAGTCTACGC TGGCTTTTTG GTGTGGCAGC TGTGGTAACT 
GATGTTGGAC AGATCCTTCT TATTGACCTG TGTCTGGATG ACTTGTCATG CAGTCAGAAT 
GAAGTAGAGG CGTCAGACCT CGAAGTCATC ACTGGCATCC CAGCTGAAGT CCCACACATC 
AGAGAAAATG TCATGCGAGA AGGGCGCCAC CTGTGTTTCC AGTTGGTGGG CCCATCAGGG 
ACAGCTGTTT CAACTCTTAG TTATGTACAC AGGACAAATC AGCTTGCTGT AGGTTTTTCT 
GATGGCTATC TAGCACTTTG GAATATGAAA AGCATGAAAA GAGAATACTA CACACAATTG 
GAAGGTGGGA GAGTTCCTGT ATATGCTGTC ACTTTCCAGG AACCTGAGAA TGATCCTCGT 
AATTGCTGTT ACTTGTGGGC TGTTCAGTCT ACACAAGATG GGGAAGGTGA TGTTTTGAGT 
TTACATCTGC TTCAGCTGGC CTTTGGTGAT AGAAAGTGTT CGGCATCAGG ACAAACCTTA 
TATGAGGGGT TAGAGTACTG TGAAGAAAGA TACACACTGG ACCTTACAGG TGGCATGTTT 
CCTTTGAGGG GACAGACTAA TAATACCAAG TTGTTGGGAT GCCAGAGTAT AGAGAAATTT 
CGATCTCATG GTGACAGGGA GGAAGGCACA AGTGAAGTTC TGTCTCCTGA CACCAGTGTT 
TCAGTCTTTA CCTGGCAAGT GAATATATAT GGACAAGGAA AGCCTTCTAT ATATTTGGGG 
CTTTTTGACA TAAATCGTTG GTATCATGCA CAAATGCCAG ATTCGTTAAG GTCGGGAGAA 
TATCTACATA ATTGCTCTTA TTTTGCATTT TGGTCACTGG ATTCTGTTGT AAGTAGGACT 
TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT 
CCTTCATATC CACCTCCTGA GCAGTTTTTT TACCCAAGTA CCTATAATTT TGATGCCACT 
TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAGAA GGAGACTTTG 
ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGCCA TTCCCGATGG TTATAATCGA 
TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATGGATA TTCAGCCTTC CAGTTTAAGT 
CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC ATACAAGTTC CCTGGGACTT 
TTGACTGGAT GCATCAAAAG TTGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTG 
CGCTTTGTTC TTGAATGGAC ATGGAATAAA GTGATTTTCA CAAAAGAGGA ATTTGACAGA 
CTGTGTATAC CATTATTTGA TGGTTCGCGT CGTTTCATTG ATCCACAAAC TATACAGTCT 
ATTCAACAGT GCAGCTTGCT TCTTAGCAAC CTTAATACAG TCTTAAGCTG TTTTGCTGCA 
GAGGCCCAAG ATATCACTGA AAGAGGTGCT GTGAACTTAA GCAATAAGTA TATGGTTTCC 
CAGCTCATCT GTCAATATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG GCTTTTACCA 
GAGGGCTTAG ATGATGCTGT GCAGTTATCA AGGTTATGCT ACAACTACCC TGTAATTCAG 
AACTACTACA CCGGTCGTCG ACAGAAGTGT GAGCATTTAT CAAGAGGGAA GTGGAACTCT 
GATTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTCGA GAAGTTGTGG 
AAACGGGATA AAGGAGGCAC AGGAAAATAT CCTCCTACTA GTCTGCATGC ATTACTTGAC 
ATTTACCTAC TAGACAACAT TACTGAAGCA AGCAAACATT CTATTACCAT ATATTTGCTA 
CTTGATATTA TGTATTCCTT CCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT 
GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GGTTTTGGCT GATAGATCAT 
AGTGACTATG AGAGTGGTTT GGATCTTCTC TTTCACCCAG CTACTACAAA ACCTGTGTCA 
TGGCAGCATT CAAAGATTAT TCAAGCCTTC TTGAGTCAGG GTGAGCACAG ACAAGCCCTC 
AGATATATTC AAACAATGAA GCCAGCAGTG TCCAGTGATA GTGATATTGT CCTTCTCCTC 
ACTGTTTTGC TTTTTAATAG GTGCATGGTT GAGGCCTGGA GTTTACTGCG ACAGCATTCC 
AGTAGGTCGA ACATAGAGGA GTTACTAAAG CACACGTATG ACGTCTGTCA GGAAATGGGC 
TTGATGGAGG ATTTACTGAA GTTACCATTT ACAGACACGG AGCAGGAATG TTTGGTGAGA 
TTTTTGCAGT CCAGCACAAG TTTTCAGAAT CAGGAATTCC TTTTAGTTCA CCATTTGCAG 
CGTGCCAATT ACATCCCTGC CTTGAAGCTG AACCAAACTC TGAAGATTAA TGCTATGAAT 
GATCGTGATC CTCGTTTGCG GGAGAGATCA GTGACTCGAA ATTCTATATT AGATCAGTAT 
GGGAAAATCC TTCCAAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCTAA GCCTTACTGT 
CTGTCAAAAT CATCAGTCCT TCGAGAAGTT TCTCGGCCCA AACCATTATC AGCAGTGCCA 
AAGCAAGCTG TAATAGGGAC TGTGTTAACA AGATCCACTT TCATCAGTAA TGTGTTATCT 
AGAATTGGAG AGGTTTGGAC AAGCAGTGAA CCTAAAAATA ACATCTTAAC CTACAATAGT 
CCTAGGGTAG AAGTACCATC TCCTATAGTA TATTCTTTCC CGGATCTAGA GCTGCCTGAG 
GCATTTGTTG GAACACCCAT TGGAAAAGCA TCACAAAGAG TTTCTAGACT CCTGGATTTG 
GTGGTTCATC CTGTTCCCCA GCTTTCTCAA TGTTTGGGGT TTATTCAGCA AACCACCCCC 
ACAAGATCTC CTTTGTACCT AACATCCAGT TCATTGCCAG AAAGTCCACA ATTAAAAGGA 
TCACGTCCAA ATACCTCCAG GGCTTCAGAA TTAAATTTAC TTGAAACTCC TCTTGTAGTT 
AAGAAAGCTA AAACTTTGGC CATGTCAGTT GCTTCCTCTG GATTTGCTGA GTTCACTCCT 
CAATCCATCC TAAGGTCTGG TCTTCGATCA ACACCTTTAG CATCTCCTTC TGTGTCACCT 
GGAAGGTCTC TTACTCCTCC TCTACGACTT AAAGAAACTA GAATTTCATT CATGGAAGAA 
GGCATGACCA CAAAATGGAT TGCTGGAGCT GCAAATGATA GCAAAACAAA AACCTTTGTT 
ACTACCCCTT TCTATAAATG TGGAGTTCTA GCAGAAACTG AATGGCCAAA GAACAAAGAT 
AAGACACCAT CTTTTTACCT GAATAGCCCT GAAATGGACC ATCGAGAAAT GGATGTGAGG 
TCACAGGATA CACATTCACA AAGTTTGGAG AAACTGGATG TGAGCAAAGA AAATAGCAAT 
GCTTCATCCG TATCTGAGCA GACTACCTCA GAGTATCAGG ATGCACCGTC ACCAGGAGAG 
TTTGAAATGT GCGTGGCCTC TAGGCCAGAG AACTCTTCCA CTGAACTAAT TACTAATTTA 
ACTGAAGAAC CTGAGAAGGA TAGTGATAAA GATGTGTTTA AATTCGAAGT AACTCCTCTA 
GACCTGGAGA AACAAATGGG CACTTTAGAA GATGCAGAAA CAAAGGCTCT CTTAGCTGCA 
GAGAAAGTGC TTTCAGAATT AAATCACTCA AACCCTATTC AAGGAGTTGA AGCTTCTGTT 
TGTGTACCAT CAATCCATGA AGGGAAAATT CTCACCCTGA AGTCCCAGGT ACCAGTTTCA 
GATAAAGCAT CAGTCGAAGC CTGCACCTCT ACAGTTAGAG CAGCTACGGC CGATGTCCTT 
GGTGATAGTG GAAGCTCTGG GCTCATTACC TCTGAAAGTC CTGTTATCTC CAAACATAGG 
CTTGACCAGA AAATAGCTTT GAACATAAAA GAAGTTCATG AACTAGAAGC TGGTGCACTA 
AAAGCAAGTG TTGACTTACC AGAAGAAAAG CCTCCAATTT CTGATGATCC GCCTGATACT 
CAAGAAATTC ATGTGATCGA ACATGAAAAG CTTGAAGTTC AAGATTCAGA ACAAGAGGCT 
AGGAGTCTTT CGTTTCATGA GTATCCCTCA GGAACTCTTA AGCTTCAATA TAATTTTGAG 
ACCATAGAGC AACAGTTCTG TGACTTGCCT GAAAACAAAG ACTCTGCTGA GTGTGACATT 
GCTGAAGTAG ACGGAGAACT TTTTGTGGCT CAGAGCAACT TTACCTTGAT ATTGGAAGGT 
GAAGAAGGAG AAGTTGAGAC AGGCGATTCT GCAGCATCTG ATATGTTAGC TAAAGCAGCT 
AACATAGCAG CTGAGGAAAA ACATGTATAC GCTGGGGAAA ATGATAATTG TGGGCATGTT 
GCTAAATTGC CATCGATCAT GACTAGTGAT CAAGAGTCCC AGAAGGTAGA GACATTACCA 
TATGTGCCTG AACCAATTAA AGTGGCAATT GCAGAAAATT TACTAGATGT AATTAAAGAC 
ACACGAAGTA AAGAAATTAC TTCAGAGACA GTGGAACAGT CCATTCATGA AAACATACCT 
CTAATAAGCC AGAAAGTAGT GTCTTCCACC AAGTTAGTCA AATCTACTTT TAAGACTGTT 
CAGGAAACAA GTACGGTGAC TTTAAATGTC AGTCAGTCTG ATGACATGGT TTCCTCCAGA 
ACTCGCACAA GAGCACGTGT CCAAAGCCAA GATGTCAGAT CAGTATCAGT GCAACAGGAA 
GAGTCAGCAG ATGTTGCTAC TCCTGAAACG CCAGGGCTTT CAGTTAAGAA GAAAACTAGG 
AAAAAAGCAG ACATTTCTGA GGCTTCCGAA AGTGCCTTTT CTGACGTCAA AGAAATATCT 
CAGAATCGGC AAATACCTCA AAATTCTATT ACTCCTAGGA GAGGAAAGAG AAAGAAAGAA 
ATTAATCAAG ACACATTAGA AAGTATTAAT TCTGTCGAAC GAGAATTACA GGTTACTCCA 
GGTAGGGAAT TAAGAAGGTT GAGATCATCG CAGCTGTTGG AACCGTTAGC TAAAGAAACT 
TCTTTTAGAA AAGAAGTTAA GCTTTCATCT GTTACGAAAC AGACTCCTAA AAGAATTAAA 
AAATCTGTAG AAAATCAGGA AAGTGTTGAA ATTATAAATG ATCTAAAAGT TAGTAAAGTA 
GCAGATCCTA GCAGAAGCAC CAGAAAATTG AGACGTGCTG GTTTAGAAGC TTCTGAAGAT 
AAAGGACATG GACAAGATGG GAAGTCCAGT GAACAACCGC CTGTTCAGCT TAGTAGTAAA 
AGAGTTAAAA AGGGAGATGT GAGTGCATCA GATGTTATAG AAAACTCTAA ACTTGATTCA 
TCACAGTTGA CTCTTCAAGC AGAATTTGAT ATGCCCGCTA CACCTAGGAA ACGTGGTAGA 
CCAAGAAAAC TTCATCCATC ACAGGATGTA GGATCCGAGG CTGTTAAGGA AGAGAGAAGT 
CCCAAGAAGA GCAAAGCTCT CAGCATGCCA AGGAGATCTG CAAGAAACAC CCCAGCTAAA 
AGTGAAAGTA CTGGTGATGG AAAACCAGCT TTAGAAGAAT CCGTTTTCGT GCCAAATGAA 
CTTGCTACAG TGATGAGCTC TAAGAAAAAG ATTACAAAAA AGACTGAAAC TCAAGGCCAG 
AAACGTTCCT GTCACTCAAT ACCAGAAAAA GGCATAGATG AAGAAATGAC AGACGAACCA 
AATGACCAAA AAGAAAAAAT GCTTGCCACT CCTTTGACTA GATCTTCCCG TCGCACAAGG 
ACTAGACCCA GCAAGGCTGG CTTGATGCCT GACCTTTCTG AACCAAAAGA CGAGTCTTTA 
CTTTCTCCTG TGCCAAAAGT CCCAAGGAAA GAGAAAGATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AHCTF1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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