LOC18104229 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18104229
Entrez Gene ID	18104229
Full Name	F5A9.22 family protein
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024584131.1	XP_024439899.1	THO complex subunit 2 isoform X1
XM_024584132.1	XP_024439900.1	THO complex subunit 2 isoform X2
XM_006375980.1	XP_006376042.1	F5A9.22 family protein
XM_024584134.1	XP_024439902.1	THO complex subunit 2 isoform X4
XM_024584133.1	XP_024439901.1	THO complex subunit 2 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18104229 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18104229 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa28640	XM_006375980.1 Latest version!	Populus trichocarpa F5A9.22 family protein (POPTR_0013s08280g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa442340	XM_024584131.1 Latest version!	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa442341	XM_024584132.1 Latest version!	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa442342	XM_024584133.1 Latest version!	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa442343	XM_024584134.1 Latest version!	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18104229 ( XM_006375980.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006376042.1 Populus trichocarpa F5A9.22 family protein (POPTR_0013s08280g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa28640
Clone ID Related Accession (Same CDS sequence)	XM_006375980.1
Accession Version	XM_006375980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	F5A9.22 family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008479).

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAGTGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA ACAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGAGAAG AAATGGTAAC TGTTATTCAG CCTTTTATGT GCTTCTTATA 
CTGTGCATAT TGGTAACTAG AATCAAAAGT GATGAAAGGG AGGATCTCAA GGTTTTGGCT 
ACAGGTGTTG CTGCAGCTTT GGCTGCTAGA AAGCCTTCAT GGGTTACAGA TGAAGAATTT 
GGCATGGGAT ATCTTGACAT AAAGCCTCCA TCTGTTGCAT CAAAGTCTTT ATCTGGAAAT 
GTAGCAGCAG CACAAAACAG TTCTGCCCTT AATGTTTCTC AAGGTGAACC TGCTGATGGA 
AGAGCACTTG TAACTGGAAG CCAACATGGA GATCCTGGGA ATTCAAACAG AGATCCGATA 
TCCAGAGCAA AACATGCTGA TGGGAGGTCA GATAGAACAG AAAATATCTC ACATTTGAAA 
TCTGATCTAG GACATCAGAA ATCAAAAGGT GCATCAAGAT CAGCAGAAAA TCAGAAGGGG 
ATGGATGACT CCACAAATAG AACATTGGAG GATAGCACTG TCAGAGTTGC TGCAAAGAAC 
TTGGCGGAGT CTGAGTTGAA AGTTTCCACA AAGCGGCCAG TGTCTAAAAC ACCAAAGCAA 
GATGTTGTGA AAGATGACAA TAAATCTGGA AAAGGTGTTG GCCGAACTCT ATCCTCATCC 
ACAAGTGACA AAGACATCCA GGTTCATTTA TCAGAGGGAA GACAAGGGGG TGCTTCTAAT 
GTTTCTTCTG TGCTCACATC GAATGAATCA AAGCCAGATA GTGGAGGTAA CAAACCTATG 
CTAAAAGATG AAGCAACTGA AGTTGCTGAT GTGCAGAAGC CACCTTCTCG ACTTGTTCAT 
TCTCCTAGGC ATGATAACTC TGTTGCTGCT TCAAAGTCTA GTGATAAATT GCAAAAGAGA 
GCAAGTCCTG CTGAAGAACC TGACAGATTA AGTAAAAGGC AAAAAGGGGA TGTCGAATTG 
AGAGATTTAG AGGGTGAAGT TAAATTCTCT GAAAGAGAGA GGTCCACCGA TACTCGATCT 
GCGGATCTTG ATAAAGTGGG AAATGATGAA CATAATCTAT ATAGGAGCGT AGATAAACCT 
TTGGATAGGT CAAAAGATAA AGGTAATGAT AGATATGACA GGGACCATAG GGAAAGATCA 
GAGCGTCCTG ATAAATCCCG TGGAGATGAT AGTTTAGCTG ACAGATCGAG AGATAAATCA 
ATGGAAAGAT ATGGAAGAGA GCTTTCGGTT GAGAGAGGGC AGGATAGGGT CGCTGATAGG 
AGTTTTGACA GACTAGCTGA CAAAGCCAAA GATGACAGAA GCAAATTACG TTACAATGAT 
ACCTCTGCAG AAAAATCTCA GGTTGATGAT CGTTTTCATG GGCAAAACTT GCCTCCGCCA 
CCACCTTTAC CTCCTCATAT GGTTCCCCAA TCAGTTACTT CTGGTAGAAG AGATGAAGAT 
GCTGACAGAA GGTTTGGAAC TACCAGACAT GTTCAGAGGC TTTCTCCTAG GCATGATGAG 
AAAGAACGAA GACGATCAGA AGAAAATTCC TTGGTTTCAC AGGATGATGC AAAACGTAGA 
AAAGAGGATG ATGTTCGTGA GAGAAAGCGA GAAGAGCGAG AGGGGCTGTC AATCAAGGTG 
GAAGAGAGGG AAAGAGAGAG GGAGAGGGAG AAAACCAACC TTCCGAAGGA GGAAATGGAC 
TCCAGTGCAA CTGCCAAGAG ACGAAAACTT AAAAGAGATC ATCTGCCGAC AGGGGAAGCT 
GGTGAATATT CTCCTGTTGC CCCACCACCT CCACCCCTTG GTATTGGCAT TTCACATTCA 
TATGATGGTA GGGAGAGGGG AGATAGGAAG GGGGCCATGA ATCAGCGTGC AAGTTACTTG 
GAGGAACCTC TCATGAGGAT TCATGGTAAA GATGTAGTTG GCAAGATGGG CCGCCGTGAT 
ACTGATCCTA TGTATGATCG GGAATGGGAT GAGGACAAGA GGCAGAGAGC TGAACAAAAG 
CGGAGGCATC GCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006376042.1
CDS	123..5540
Translation	MSTTLPPMECLHVTEEFLLELKSGNRSFRLPHPVPILRFLYELSWTLVRGELPFQKCKAALDSVEFVDKMSAVGLGSNFADIITQMAQDLTMSGEYRSRLIKLAKWLVESALVPLRFFQERCEEEFLWEAEMIKIKAQDLKGKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCRGSEDTAENTSAATIGIIKSLIGHFDLDPNRVFDIVLECFELQPDSNVFLELIPIFPKSHASQILGFKFQYYQRMELNSPVPFGLFKLTALLVKEEFIDLDSICAHLLPKDDEAFEHYNTFSSKRLDAAYKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMETEAVAEQFSDLEKNQTLGLLTGFLSVDDWYHAHILFKRLSPLNPVAHTQICGGLFRLIEKTISSAYNIIRQPHVQNCGSPAVAGIDAMDVTSSSGHVSLIDLPKEFFQMLVTVGPYLYRDTLLLQKVCRVLRGYYMSALELVDSGDGALNGESLIPRNRVLRLHLREGRSMVEEALGACLLPSLQLVPANPAAGQEIWEVMSLLPYEVRYRLYGEWEKDDERNPLVLAARQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMITPVVDAFKYLTQLEYDILEYVVIERLAQGGRDKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGQGIELVLLQELVQQMANVQYTENLTEEQLDAMAGSETLRYQATSFGVTRNNKALFKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVINADAPYIKMVSEQFDRCHGTLLQYVEFLCGAVTPPSAYAQLIPSLDDLVHLYHLDPEVAFLIYRPVMRLFKCEGSLEVFWPLDSSETVTTTSANLEPEAREYSGRVILDLGSLHKPVMWSDLLETLKTMLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQQAALKALEELSDNSSSAITKRKKEKERIQESLDRLTSELHKHEENVSSVRRRLSREKDKWLTSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVHTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEAGRLGRFLYETLKIAYYWKSDETIYERECGNMPGFAVYYRFPNSQRVTYGQFIKRRNGNCYSAFYVLLILCILVTRIKSDEREDLKVLATGVAAALAARKPSWVTDEEFGMGYLDIKPPSVASKSLSGNVAAAQNSSALNVSQGEPADGRALVTGSQHGDPGNSNRDPISRAKHADGRSDRTENISHLKSDLGHQKSKGASRSAENQKGMDDSTNRTLEDSTVRVAAKNLAESELKVSTKRPVSKTPKQDVVKDDNKSGKGVGRTLSSSTSDKDIQVHLSEGRQGGASNVSSVLTSNESKPDSGGNKPMLKDEATEVADVQKPPSRLVHSPRHDNSVAASKSSDKLQKRASPAEEPDRLSKRQKGDVELRDLEGEVKFSERERSTDTRSADLDKVGNDEHNLYRSVDKPLDRSKDKGNDRYDRDHRERSERPDKSRGDDSLADRSRDKSMERYGRELSVERGQDRVADRSFDRLADKAKDDRSKLRYNDTSAEKSQVDDRFHGQNLPPPPPLPPHMVPQSVTSGRRDEDADRRFGTTRHVQRLSPRHDEKERRRSEENSLVSQDDAKRRKEDDVRERKREEREGLSIKVEEREREREREKTNLPKEEMDSSATAKRRKLKRDHLPTGEAGEYSPVAPPPPPLGIGISHSYDGRERGDRKGAMNQRASYLEEPLMRIHGKDVVGKMGRRDTDPMYDREWDEDKRQRAEQKRRHRK

Target ORF information:

RefSeq Version	XM_006375980.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa F5A9.22 family protein (POPTR_0013s08280g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006375980.1

ORF Insert Sequence:

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAGTGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA ACAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGAGAAG AAATGGTAAC TGTTATTCAG CCTTTTATGT GCTTCTTATA 
CTGTGCATAT TGGTAACTAG AATCAAAAGT GATGAAAGGG AGGATCTCAA GGTTTTGGCT 
ACAGGTGTTG CTGCAGCTTT GGCTGCTAGA AAGCCTTCAT GGGTTACAGA TGAAGAATTT 
GGCATGGGAT ATCTTGACAT AAAGCCTCCA TCTGTTGCAT CAAAGTCTTT ATCTGGAAAT 
GTAGCAGCAG CACAAAACAG TTCTGCCCTT AATGTTTCTC AAGGTGAACC TGCTGATGGA 
AGAGCACTTG TAACTGGAAG CCAACATGGA GATCCTGGGA ATTCAAACAG AGATCCGATA 
TCCAGAGCAA AACATGCTGA TGGGAGGTCA GATAGAACAG AAAATATCTC ACATTTGAAA 
TCTGATCTAG GACATCAGAA ATCAAAAGGT GCATCAAGAT CAGCAGAAAA TCAGAAGGGG 
ATGGATGACT CCACAAATAG AACATTGGAG GATAGCACTG TCAGAGTTGC TGCAAAGAAC 
TTGGCGGAGT CTGAGTTGAA AGTTTCCACA AAGCGGCCAG TGTCTAAAAC ACCAAAGCAA 
GATGTTGTGA AAGATGACAA TAAATCTGGA AAAGGTGTTG GCCGAACTCT ATCCTCATCC 
ACAAGTGACA AAGACATCCA GGTTCATTTA TCAGAGGGAA GACAAGGGGG TGCTTCTAAT 
GTTTCTTCTG TGCTCACATC GAATGAATCA AAGCCAGATA GTGGAGGTAA CAAACCTATG 
CTAAAAGATG AAGCAACTGA AGTTGCTGAT GTGCAGAAGC CACCTTCTCG ACTTGTTCAT 
TCTCCTAGGC ATGATAACTC TGTTGCTGCT TCAAAGTCTA GTGATAAATT GCAAAAGAGA 
GCAAGTCCTG CTGAAGAACC TGACAGATTA AGTAAAAGGC AAAAAGGGGA TGTCGAATTG 
AGAGATTTAG AGGGTGAAGT TAAATTCTCT GAAAGAGAGA GGTCCACCGA TACTCGATCT 
GCGGATCTTG ATAAAGTGGG AAATGATGAA CATAATCTAT ATAGGAGCGT AGATAAACCT 
TTGGATAGGT CAAAAGATAA AGGTAATGAT AGATATGACA GGGACCATAG GGAAAGATCA 
GAGCGTCCTG ATAAATCCCG TGGAGATGAT AGTTTAGCTG ACAGATCGAG AGATAAATCA 
ATGGAAAGAT ATGGAAGAGA GCTTTCGGTT GAGAGAGGGC AGGATAGGGT CGCTGATAGG 
AGTTTTGACA GACTAGCTGA CAAAGCCAAA GATGACAGAA GCAAATTACG TTACAATGAT 
ACCTCTGCAG AAAAATCTCA GGTTGATGAT CGTTTTCATG GGCAAAACTT GCCTCCGCCA 
CCACCTTTAC CTCCTCATAT GGTTCCCCAA TCAGTTACTT CTGGTAGAAG AGATGAAGAT 
GCTGACAGAA GGTTTGGAAC TACCAGACAT GTTCAGAGGC TTTCTCCTAG GCATGATGAG 
AAAGAACGAA GACGATCAGA AGAAAATTCC TTGGTTTCAC AGGATGATGC AAAACGTAGA 
AAAGAGGATG ATGTTCGTGA GAGAAAGCGA GAAGAGCGAG AGGGGCTGTC AATCAAGGTG 
GAAGAGAGGG AAAGAGAGAG GGAGAGGGAG AAAACCAACC TTCCGAAGGA GGAAATGGAC 
TCCAGTGCAA CTGCCAAGAG ACGAAAACTT AAAAGAGATC ATCTGCCGAC AGGGGAAGCT 
GGTGAATATT CTCCTGTTGC CCCACCACCT CCACCCCTTG GTATTGGCAT TTCACATTCA 
TATGATGGTA GGGAGAGGGG AGATAGGAAG GGGGCCATGA ATCAGCGTGC AAGTTACTTG 
GAGGAACCTC TCATGAGGAT TCATGGTAAA GATGTAGTTG GCAAGATGGG CCGCCGTGAT 
ACTGATCCTA TGTATGATCG GGAATGGGAT GAGGACAAGA GGCAGAGAGC TGAACAAAAG 
CGGAGGCATC GCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18104229 ( XM_024584131.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024439899.1 Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X1, mRNA.

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CloneID	OPa442340
Clone ID Related Accession (Same CDS sequence)	XM_024584131.1
Accession Version	XM_024584131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	THO complex subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037297.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGGTA ATGCTCTTTC AGTGTCTGAA AAAGTCTCTA CTATGGTTAC AAGAACTTCT 
GATTCTTATG GTGTAGAATC AAAGCCAGAT AGTGGAGGTA ACAAACCTAT GCTAAAAGAT 
GAAGCAACTG AAGTTGCTGA TGTGCAGAAG CCACCTTCTC GACTTGTTCA TTCTCCTAGG 
CATGATAACT CTGTTGCTGC TTCAAAGTCT AGTGATAAAT TGCAAAAGAG AGCAAGTCCT 
GCTGAAGAAC CTGACAGATT AAGTAAAAGG CAAAAAGGGG ATGTCGAATT GAGAGATTTA 
GAGGGTGAAG TTAAATTCTC TGAAAGAGAG AGGTCCACCG ATACTCGATC TGCGGATCTT 
GATAAAGTGG GAAATGATGA ACATAATCTA TATAGGAGCG TAGATAAACC TTTGGATAGG 
TCAAAAGATA AAGGTAATGA TAGATATGAC AGGGACCATA GGGAAAGATC AGAGCGTCCT 
GATAAATCCC GTGGAGATGA TAGTTTAGCT GACAGATCGA GAGATAAATC AATGGAAAGA 
TATGGAAGAG AGCTTTCGGT TGAGAGAGGG CAGGATAGGG TCGCTGATAG GAGTTTTGAC 
AGACTAGCTG ACAAAGCCAA AGATGACAGA AGCAAATTAC GTTACAATGA TACCTCTGCA 
GAAAAATCTC AGGTTGATGA TCGTTTTCAT GGGCAAAACT TGCCTCCGCC ACCACCTTTA 
CCTCCTCATA TGGTTCCCCA ATCAGTTACT TCTGGTAGAA GAGATGAAGA TGCTGACAGA 
AGGTTTGGAA CTACCAGACA TGTTCAGAGG CTTTCTCCTA GGCATGATGA GAAAGAACGA 
AGACGATCAG AAGAAAATTC CTTGGTTTCA CAGGATGATG CAAAACGTAG AAAAGAGGAT 
GATGTTCGTG AGAGAAAGCG AGAAGAGCGA GAGGGGCTGT CAATCAAGGT GGAAGAGAGG 
GAAAGAGAGA GGGAGAGGGA GAAAACCAAC CTTCCGAAGG AGGAAATGGA CTCCAGTGCA 
ACTGCCAAGA GACGAAAACT TAAAAGAGAT CATCTGCCGA CAGGGGAAGC TGGTGAATAT 
TCTCCTGTTG CCCCACCACC TCCACCCCTT GGTATTGGCA TTTCACATTC ATATGATGGT 
AGGGAGAGGG GAGATAGGAA GGGGGCCATG AATCAGCGTG CAAGTTACTT GGAGGAACCT 
CTCATGAGGA TTCATGGTAA AGATGTAGTT GGCAAGATGG GCCGCCGTGA TACTGATCCT 
ATGTATGATC GGGAATGGGA TGAGGACAAG AGGCAGAGAG CTGAACAAAA GCGGAGGCAT 
CGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024439899.1
CDS	165..5813
Translation	MSTTLPPMECLHVTEEFLLELKSGNRSFRLPHPVPILRFLYELSWTLVRGELPFQKCKAALDSVEFVDKMSAVGLGSNFADIITQMAQDLTMSGEYRSRLIKLAKWLVESALVPLRFFQERCEEEFLWEAEMIKIKAQDLKGKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCRGSEDTAENTSAATIGIIKSLIGHFDLDPNRVFDIVLECFELQPDSNVFLELIPIFPKSHASQILGFKFQYYQRMELNSPVPFGLFKLTALLVKEEFIDLDSICAHLLPKDDEAFEHYNTFSSKRLDAAYKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMETEAVAEQFSDLEKNQTLGLLTGFLSVDDWYHAHILFKRLSPLNPVAHTQICGGLFRLIEKTISSAYNIIRQPHVQNCGSPAVAGIDAMDVTSSSGHVSLIDLPKEFFQMLVTVGPYLYRDTLLLQKVCRVLRGYYMSALELVDSGDGALNGESLIPRNRVLRLHLREGRSMVEEALGACLLPSLQLVPANPAAGQEIWEVMSLLPYEVRYRLYGEWEKDDERNPLVLAARQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMITPVVDAFKYLTQLEYDILEYVVIERLAQGGRDKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGQGIELVLLQELVQQMANVQYTENLTEEQLDAMAGSETLRYQATSFGVTRNNKALFKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVINADAPYIKMVSEQFDRCHGTLLQYVEFLCGAVTPPSAYAQLIPSLDDLVHLYHLDPEVAFLIYRPVMRLFKCEGSLEVFWPLDSNETVTTTSANLEPEAREYSGRVILDLGSLHKPVMWSDLLETLKTMLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQQAALKALEELSDNSSSAITKRKKEKERIQESLDRLTSELHKHEENVSSVRRRLSREKDKWLTSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVHTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEAGRLGRFLYETLKIAYYWKSDETIYERECGNMPGFAVYYRFPNSQRVTYGQFIKVHWKWSQRMSRLLIQCLESSEYMEIRNALILLTKISGVFPVTKRSGINLEKRVTRIKSDEREDLKVLATGVAAALAARKPSWVTDEEFGMGYLDIKPPSVASKSLSGNVAAAQNSSALNVSQGEPADGRALVTGSQHGDPGNSNRDPISRAKHADGRSDRTENISHLKSDLGHQKSKGGSSTNGSNAQSAVSSAAVPIGASRSAENQKGMDDSTNRTLEDSTVRVAAKNLAESELKVSTKRPVSKTPKQDVVKDDNKSGKGVGRTLSSSTSDKDIQVHLSEGRQGGASNVSSVLTSNGNALSVSEKVSTMVTRTSDSYGVESKPDSGGNKPMLKDEATEVADVQKPPSRLVHSPRHDNSVAASKSSDKLQKRASPAEEPDRLSKRQKGDVELRDLEGEVKFSERERSTDTRSADLDKVGNDEHNLYRSVDKPLDRSKDKGNDRYDRDHRERSERPDKSRGDDSLADRSRDKSMERYGRELSVERGQDRVADRSFDRLADKAKDDRSKLRYNDTSAEKSQVDDRFHGQNLPPPPPLPPHMVPQSVTSGRRDEDADRRFGTTRHVQRLSPRHDEKERRRSEENSLVSQDDAKRRKEDDVRERKREEREGLSIKVEEREREREREKTNLPKEEMDSSATAKRRKLKRDHLPTGEAGEYSPVAPPPPPLGIGISHSYDGRERGDRKGAMNQRASYLEEPLMRIHGKDVVGKMGRRDTDPMYDREWDEDKRQRAEQKRRHRK

Target ORF information:

RefSeq Version	XM_024584131.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024584131.1

ORF Insert Sequence:

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGGTA ATGCTCTTTC AGTGTCTGAA AAAGTCTCTA CTATGGTTAC AAGAACTTCT 
GATTCTTATG GTGTAGAATC AAAGCCAGAT AGTGGAGGTA ACAAACCTAT GCTAAAAGAT 
GAAGCAACTG AAGTTGCTGA TGTGCAGAAG CCACCTTCTC GACTTGTTCA TTCTCCTAGG 
CATGATAACT CTGTTGCTGC TTCAAAGTCT AGTGATAAAT TGCAAAAGAG AGCAAGTCCT 
GCTGAAGAAC CTGACAGATT AAGTAAAAGG CAAAAAGGGG ATGTCGAATT GAGAGATTTA 
GAGGGTGAAG TTAAATTCTC TGAAAGAGAG AGGTCCACCG ATACTCGATC TGCGGATCTT 
GATAAAGTGG GAAATGATGA ACATAATCTA TATAGGAGCG TAGATAAACC TTTGGATAGG 
TCAAAAGATA AAGGTAATGA TAGATATGAC AGGGACCATA GGGAAAGATC AGAGCGTCCT 
GATAAATCCC GTGGAGATGA TAGTTTAGCT GACAGATCGA GAGATAAATC AATGGAAAGA 
TATGGAAGAG AGCTTTCGGT TGAGAGAGGG CAGGATAGGG TCGCTGATAG GAGTTTTGAC 
AGACTAGCTG ACAAAGCCAA AGATGACAGA AGCAAATTAC GTTACAATGA TACCTCTGCA 
GAAAAATCTC AGGTTGATGA TCGTTTTCAT GGGCAAAACT TGCCTCCGCC ACCACCTTTA 
CCTCCTCATA TGGTTCCCCA ATCAGTTACT TCTGGTAGAA GAGATGAAGA TGCTGACAGA 
AGGTTTGGAA CTACCAGACA TGTTCAGAGG CTTTCTCCTA GGCATGATGA GAAAGAACGA 
AGACGATCAG AAGAAAATTC CTTGGTTTCA CAGGATGATG CAAAACGTAG AAAAGAGGAT 
GATGTTCGTG AGAGAAAGCG AGAAGAGCGA GAGGGGCTGT CAATCAAGGT GGAAGAGAGG 
GAAAGAGAGA GGGAGAGGGA GAAAACCAAC CTTCCGAAGG AGGAAATGGA CTCCAGTGCA 
ACTGCCAAGA GACGAAAACT TAAAAGAGAT CATCTGCCGA CAGGGGAAGC TGGTGAATAT 
TCTCCTGTTG CCCCACCACC TCCACCCCTT GGTATTGGCA TTTCACATTC ATATGATGGT 
AGGGAGAGGG GAGATAGGAA GGGGGCCATG AATCAGCGTG CAAGTTACTT GGAGGAACCT 
CTCATGAGGA TTCATGGTAA AGATGTAGTT GGCAAGATGG GCCGCCGTGA TACTGATCCT 
ATGTATGATC GGGAATGGGA TGAGGACAAG AGGCAGAGAG CTGAACAAAA GCGGAGGCAT 
CGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18104229 ( XM_024584132.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024439900.1 Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa442341
Clone ID Related Accession (Same CDS sequence)	XM_024584132.1
Accession Version	XM_024584132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	THO complex subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037297.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTATTATCA GCGAATGGAA 
TTAAATAGCC CTGTACCATT TGGGCTCTTT AAGCTTACAG CCTTACTAGT GAAGGAAGAG 
TTCATTGATC TTGATAGTAT ATGTGCACAT TTACTCCCTA AGGATGATGA GGCTTTTGAG 
CATTATAACA CGTTTTCTTC CAAACGATTG GATGCGGCCT ACAAAATTGG AAAAATAAAT 
CTGGCTGCTA CTGGAAAGGA CCTCATGGAT GATGAGAAGC AAGGAGATGT GACAGTAGAT 
CTTTTTGCTG CCTTAGACAT GGAAACTGAG GCAGTAGCTG AACAATTCTC TGATCTTGAA 
AAAAATCAAA CTTTGGGCTT GCTGACTGGT TTCCTTTCTG TGGATGACTG GTATCATGCC 
CATATACTTT TCAAACGTCT CTCTCCTCTA AATCCTGTTG CACATACTCA AATATGTGGT 
GGCTTGTTTA GGCTCATTGA AAAGACGATC TCATCAGCAT ATAATATTAT TCGTCAACCA 
CATGTTCAAA ATTGTGGGTC TCCAGCAGTT GCTGGTATTG ATGCCATGGA TGTGACAAGT 
TCTTCAGGCC ATGTCTCTTT AATTGATCTT CCCAAAGAAT TTTTTCAGAT GCTTGTAACA 
GTTGGACCTT ATCTTTACCG TGATACGTTA TTGCTGCAGA AGGTTTGTAG AGTGTTAAGA 
GGTTACTATA TGTCTGCACT TGAGCTTGTA GATAGTGGTG ATGGAGCTCT AAATGGTGAA 
TCTCTTATAC CTAGAAATCG AGTTCTTCGT TTGCACCTAA GGGAAGGTAG GTCAATGGTT 
GAGGAAGCCC TGGGAGCATG TTTACTTCCT TCTTTACAGT TGGTACCTGC AAATCCAGCA 
GCTGGGCAGG AGATCTGGGA AGTAATGAGT CTCCTTCCAT ATGAGGTGCG ATATCGTTTA 
TATGGTGAAT GGGAAAAAGA TGATGAGCGG AATCCATTGG TTTTGGCTGC AAGGCAAACA 
GCCAAGTTGG ATACTAGAAG GATATTGAAA AGGCTTGCGA AGGAAAATTT GAAGCAGCTG 
GGTAGGATGG TTGCAAAATT AGCGCATGCT AACCCGATGA CTGTCCTTCG AACTATTGTT 
CATCAGATTG AGGCATACAG GGATATGATC ACTCCTGTTG TGGATGCTTT CAAGTATTTG 
ACACAGCTTG AGTATGATAT CTTGGAATAT GTTGTAATTG AGCGGTTAGC ACAAGGTGGT 
CGTGATAAGC TCAAGGATGA TGGCCTTAAT TTGTCAGACT GGCTTCAATC ACTGGCTTCA 
TTCTGGGGTC ACCTGTGTAA GAAGTACCCA TCTATGGAAT TGAGAGGCCT TTTCCAGTAT 
TTAGTGAACC AGTTGAAAAA GGGCCAGGGG ATTGAGCTTG TTCTTCTGCA GGAGCTTGTT 
CAACAAATGG CAAATGTTCA ATACACAGAA AACTTAACAG AGGAACAGTT GGATGCCATG 
GCAGGGAGTG AGACACTTCG GTATCAAGCT ACTTCTTTTG GAGTAACACG GAATAACAAG 
GCGTTGTTCA AATCTACTAA CAGGCTTCGA GACTCCTTGC TTCCTAAGGA TGAACCAAAG 
CTAGCGATCC CCCTTTTGTT ACTTATTGCT CAACATCGAT CTGTGGTTGT CATAAATGCA 
GATGCTCCTT ACATTAAAAT GGTCAGTGAG CAGTTTGATA GGTGTCATGG AACTCTTCTT 
CAATATGTGG AATTCCTCTG TGGTGCTGTG ACACCACCCT CTGCATATGC TCAGTTAATT 
CCCTCTCTTG ATGATCTTGT CCACCTGTAT CATCTTGATC CTGAGGTTGC ATTTTTAATA 
TACCGCCCAG TCATGAGACT CTTCAAGTGT GAGGGTAGTT TAGAGGTTTT CTGGCCCTTA 
GACAGCAATG AAACAGTAAC TACTACAAGT GCAAACCTGG AGCCTGAAGC TAGAGAATAT 
TCTGGCAGAG TGATTCTGGA TCTTGGCTCT CTCCATAAGC CTGTAATGTG GTCAGATCTT 
CTTGAGACTC TGAAAACCAT GTTGCCTTCA AAAGCCTGGA ATAGCCTCTC ACCTGATCTC 
TATGCCACTT TCTGGGGACT CACACTTTAT GATCTATATG TCCCTAGAAA CCGTTACGAG 
TCTGAAATTG CCAAGCAGCA AGCTGCTCTT AAAGCGCTGG AAGAGCTTTC TGATAATTCT 
AGTTCAGCAA TTACTAAAAG GAAGAAAGAG AAGGAAAGGA TTCAAGAATC TCTCGATCGT 
CTGACTAGTG AGCTCCATAA ACATGAAGAA AATGTTTCTT CTGTTCGTAG ACGACTATCT 
CGTGAGAAGG ACAAATGGCT GACTTCTTGT CCTGATACTT TGAAAATCAA CATGGAGTTC 
CTTCAGCGCT GTATTTTTCC CCGTTGTACT TTCAGCATGC CGGATGCTGT ATATTGTGCC 
ATGTTTGTAC ATACTCTTCA TTCACTTGGC ACACCATTTT TCAACACTGT AAACCACATC 
GATGTTCTGA TATGTAAAAC ATTACAACCT ATGATATGCT GCTGTACTGA ATATGAAGCA 
GGTAGACTTG GGAGGTTTTT GTATGAAACA CTCAAGATTG CTTACTACTG GAAGAGTGAT 
GAAACTATAT ATGAACGTGA GTGTGGAAAT ATGCCTGGAT TTGCTGTGTA TTATAGATTC 
CCAAACAGCC AACGGGTTAC ATATGGTCAG TTCATTAAGG TACACTGGAA ATGGAGTCAG 
AGAATGTCTC GGCTATTGAT TCAGTGTCTG GAATCATCTG AGTACATGGA AATTCGAAAT 
GCTCTTATTT TGTTGACAAA AATTTCCGGT GTTTTTCCAG TTACTAAAAG GAGTGGTATT 
AACCTTGAAA AACGAGTAAC TAGAATCAAA AGTGATGAAA GGGAGGATCT CAAGGTTTTG 
GCTACAGGTG TTGCTGCAGC TTTGGCTGCT AGAAAGCCTT CATGGGTTAC AGATGAAGAA 
TTTGGCATGG GATATCTTGA CATAAAGCCT CCATCTGTTG CATCAAAGTC TTTATCTGGA 
AATGTAGCAG CAGCACAAAA CAGTTCTGCC CTTAATGTTT CTCAAGGTGA ACCTGCTGAT 
GGAAGAGCAC TTGTAACTGG AAGCCAACAT GGAGATCCTG GGAATTCAAA CAGAGATCCG 
ATATCCAGAG CAAAACATGC TGATGGGAGG TCAGATAGAA CAGAAAATAT CTCACATTTG 
AAATCTGATC TAGGACATCA GAAATCAAAA GGTGGTTCAT CAACCAATGG GTCAAATGCT 
CAGTCAGCTG TTTCCTCGGC TGCTGTTCCT ATAGGTGCAT CAAGATCAGC AGAAAATCAG 
AAGGGGATGG ATGACTCCAC AAATAGAACA TTGGAGGATA GCACTGTCAG AGTTGCTGCA 
AAGAACTTGG CGGAGTCTGA GTTGAAAGTT TCCACAAAGC GGCCAGTGTC TAAAACACCA 
AAGCAAGATG TTGTGAAAGA TGACAATAAA TCTGGAAAAG GTGTTGGCCG AACTCTATCC 
TCATCCACAA GTGACAAAGA CATCCAGGTT CATTTATCAG AGGGAAGACA AGGGGGTGCT 
TCTAATGTTT CTTCTGTGCT CACATCGAAT GGTAATGCTC TTTCAGTGTC TGAAAAAGTC 
TCTACTATGG TTACAAGAAC TTCTGATTCT TATGGTGTAG AATCAAAGCC AGATAGTGGA 
GGTAACAAAC CTATGCTAAA AGATGAAGCA ACTGAAGTTG CTGATGTGCA GAAGCCACCT 
TCTCGACTTG TTCATTCTCC TAGGCATGAT AACTCTGTTG CTGCTTCAAA GTCTAGTGAT 
AAATTGCAAA AGAGAGCAAG TCCTGCTGAA GAACCTGACA GATTAAGTAA AAGGCAAAAA 
GGGGATGTCG AATTGAGAGA TTTAGAGGGT GAAGTTAAAT TCTCTGAAAG AGAGAGGTCC 
ACCGATACTC GATCTGCGGA TCTTGATAAA GTGGGAAATG ATGAACATAA TCTATATAGG 
AGCGTAGATA AACCTTTGGA TAGGTCAAAA GATAAAGGTA ATGATAGATA TGACAGGGAC 
CATAGGGAAA GATCAGAGCG TCCTGATAAA TCCCGTGGAG ATGATAGTTT AGCTGACAGA 
TCGAGAGATA AATCAATGGA AAGATATGGA AGAGAGCTTT CGGTTGAGAG AGGGCAGGAT 
AGGGTCGCTG ATAGGAGTTT TGACAGACTA GCTGACAAAG CCAAAGATGA CAGAAGCAAA 
TTACGTTACA ATGATACCTC TGCAGAAAAA TCTCAGGTTG ATGATCGTTT TCATGGGCAA 
AACTTGCCTC CGCCACCACC TTTACCTCCT CATATGGTTC CCCAATCAGT TACTTCTGGT 
AGAAGAGATG AAGATGCTGA CAGAAGGTTT GGAACTACCA GACATGTTCA GAGGCTTTCT 
CCTAGGCATG ATGAGAAAGA ACGAAGACGA TCAGAAGAAA ATTCCTTGGT TTCACAGGAT 
GATGCAAAAC GTAGAAAAGA GGATGATGTT CGTGAGAGAA AGCGAGAAGA GCGAGAGGGG 
CTGTCAATCA AGGTGGAAGA GAGGGAAAGA GAGAGGGAGA GGGAGAAAAC CAACCTTCCG 
AAGGAGGAAA TGGACTCCAG TGCAACTGCC AAGAGACGAA AACTTAAAAG AGATCATCTG 
CCGACAGGGG AAGCTGGTGA ATATTCTCCT GTTGCCCCAC CACCTCCACC CCTTGGTATT 
GGCATTTCAC ATTCATATGA TGGTAGGGAG AGGGGAGATA GGAAGGGGGC CATGAATCAG 
CGTGCAAGTT ACTTGGAGGA ACCTCTCATG AGGATTCATG GTAAAGATGT AGTTGGCAAG 
ATGGGCCGCC GTGATACTGA TCCTATGTAT GATCGGGAAT GGGATGAGGA CAAGAGGCAG 
AGAGCTGAAC AAAAGCGGAG GCATCGCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024439900.1
CDS	165..5777
Translation	MSTTLPPMECLHVTEEFLLELKSGNRSFRLPHPVPILRFLYELSWTLVRGELPFQKCKAALDSVEFVDKMSAVGLGSNFADIITQMAQDLTMSGEYRSRLIKLAKWLVESALVPLRFFQERCEEEFLWEAEMIKIKAQDLKGKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCRGSEDTAENTSAATIGIIKSLIGHFDLDPNRVFDIVLECFELQPDSNVFLELIPIFPKYYQRMELNSPVPFGLFKLTALLVKEEFIDLDSICAHLLPKDDEAFEHYNTFSSKRLDAAYKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMETEAVAEQFSDLEKNQTLGLLTGFLSVDDWYHAHILFKRLSPLNPVAHTQICGGLFRLIEKTISSAYNIIRQPHVQNCGSPAVAGIDAMDVTSSSGHVSLIDLPKEFFQMLVTVGPYLYRDTLLLQKVCRVLRGYYMSALELVDSGDGALNGESLIPRNRVLRLHLREGRSMVEEALGACLLPSLQLVPANPAAGQEIWEVMSLLPYEVRYRLYGEWEKDDERNPLVLAARQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMITPVVDAFKYLTQLEYDILEYVVIERLAQGGRDKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGQGIELVLLQELVQQMANVQYTENLTEEQLDAMAGSETLRYQATSFGVTRNNKALFKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVINADAPYIKMVSEQFDRCHGTLLQYVEFLCGAVTPPSAYAQLIPSLDDLVHLYHLDPEVAFLIYRPVMRLFKCEGSLEVFWPLDSNETVTTTSANLEPEAREYSGRVILDLGSLHKPVMWSDLLETLKTMLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQQAALKALEELSDNSSSAITKRKKEKERIQESLDRLTSELHKHEENVSSVRRRLSREKDKWLTSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVHTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEAGRLGRFLYETLKIAYYWKSDETIYERECGNMPGFAVYYRFPNSQRVTYGQFIKVHWKWSQRMSRLLIQCLESSEYMEIRNALILLTKISGVFPVTKRSGINLEKRVTRIKSDEREDLKVLATGVAAALAARKPSWVTDEEFGMGYLDIKPPSVASKSLSGNVAAAQNSSALNVSQGEPADGRALVTGSQHGDPGNSNRDPISRAKHADGRSDRTENISHLKSDLGHQKSKGGSSTNGSNAQSAVSSAAVPIGASRSAENQKGMDDSTNRTLEDSTVRVAAKNLAESELKVSTKRPVSKTPKQDVVKDDNKSGKGVGRTLSSSTSDKDIQVHLSEGRQGGASNVSSVLTSNGNALSVSEKVSTMVTRTSDSYGVESKPDSGGNKPMLKDEATEVADVQKPPSRLVHSPRHDNSVAASKSSDKLQKRASPAEEPDRLSKRQKGDVELRDLEGEVKFSERERSTDTRSADLDKVGNDEHNLYRSVDKPLDRSKDKGNDRYDRDHRERSERPDKSRGDDSLADRSRDKSMERYGRELSVERGQDRVADRSFDRLADKAKDDRSKLRYNDTSAEKSQVDDRFHGQNLPPPPPLPPHMVPQSVTSGRRDEDADRRFGTTRHVQRLSPRHDEKERRRSEENSLVSQDDAKRRKEDDVRERKREEREGLSIKVEEREREREREKTNLPKEEMDSSATAKRRKLKRDHLPTGEAGEYSPVAPPPPPLGIGISHSYDGRERGDRKGAMNQRASYLEEPLMRIHGKDVVGKMGRRDTDPMYDREWDEDKRQRAEQKRRHRK

Target ORF information:

RefSeq Version	XM_024584132.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024584132.1

ORF Insert Sequence:

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTATTATCA GCGAATGGAA 
TTAAATAGCC CTGTACCATT TGGGCTCTTT AAGCTTACAG CCTTACTAGT GAAGGAAGAG 
TTCATTGATC TTGATAGTAT ATGTGCACAT TTACTCCCTA AGGATGATGA GGCTTTTGAG 
CATTATAACA CGTTTTCTTC CAAACGATTG GATGCGGCCT ACAAAATTGG AAAAATAAAT 
CTGGCTGCTA CTGGAAAGGA CCTCATGGAT GATGAGAAGC AAGGAGATGT GACAGTAGAT 
CTTTTTGCTG CCTTAGACAT GGAAACTGAG GCAGTAGCTG AACAATTCTC TGATCTTGAA 
AAAAATCAAA CTTTGGGCTT GCTGACTGGT TTCCTTTCTG TGGATGACTG GTATCATGCC 
CATATACTTT TCAAACGTCT CTCTCCTCTA AATCCTGTTG CACATACTCA AATATGTGGT 
GGCTTGTTTA GGCTCATTGA AAAGACGATC TCATCAGCAT ATAATATTAT TCGTCAACCA 
CATGTTCAAA ATTGTGGGTC TCCAGCAGTT GCTGGTATTG ATGCCATGGA TGTGACAAGT 
TCTTCAGGCC ATGTCTCTTT AATTGATCTT CCCAAAGAAT TTTTTCAGAT GCTTGTAACA 
GTTGGACCTT ATCTTTACCG TGATACGTTA TTGCTGCAGA AGGTTTGTAG AGTGTTAAGA 
GGTTACTATA TGTCTGCACT TGAGCTTGTA GATAGTGGTG ATGGAGCTCT AAATGGTGAA 
TCTCTTATAC CTAGAAATCG AGTTCTTCGT TTGCACCTAA GGGAAGGTAG GTCAATGGTT 
GAGGAAGCCC TGGGAGCATG TTTACTTCCT TCTTTACAGT TGGTACCTGC AAATCCAGCA 
GCTGGGCAGG AGATCTGGGA AGTAATGAGT CTCCTTCCAT ATGAGGTGCG ATATCGTTTA 
TATGGTGAAT GGGAAAAAGA TGATGAGCGG AATCCATTGG TTTTGGCTGC AAGGCAAACA 
GCCAAGTTGG ATACTAGAAG GATATTGAAA AGGCTTGCGA AGGAAAATTT GAAGCAGCTG 
GGTAGGATGG TTGCAAAATT AGCGCATGCT AACCCGATGA CTGTCCTTCG AACTATTGTT 
CATCAGATTG AGGCATACAG GGATATGATC ACTCCTGTTG TGGATGCTTT CAAGTATTTG 
ACACAGCTTG AGTATGATAT CTTGGAATAT GTTGTAATTG AGCGGTTAGC ACAAGGTGGT 
CGTGATAAGC TCAAGGATGA TGGCCTTAAT TTGTCAGACT GGCTTCAATC ACTGGCTTCA 
TTCTGGGGTC ACCTGTGTAA GAAGTACCCA TCTATGGAAT TGAGAGGCCT TTTCCAGTAT 
TTAGTGAACC AGTTGAAAAA GGGCCAGGGG ATTGAGCTTG TTCTTCTGCA GGAGCTTGTT 
CAACAAATGG CAAATGTTCA ATACACAGAA AACTTAACAG AGGAACAGTT GGATGCCATG 
GCAGGGAGTG AGACACTTCG GTATCAAGCT ACTTCTTTTG GAGTAACACG GAATAACAAG 
GCGTTGTTCA AATCTACTAA CAGGCTTCGA GACTCCTTGC TTCCTAAGGA TGAACCAAAG 
CTAGCGATCC CCCTTTTGTT ACTTATTGCT CAACATCGAT CTGTGGTTGT CATAAATGCA 
GATGCTCCTT ACATTAAAAT GGTCAGTGAG CAGTTTGATA GGTGTCATGG AACTCTTCTT 
CAATATGTGG AATTCCTCTG TGGTGCTGTG ACACCACCCT CTGCATATGC TCAGTTAATT 
CCCTCTCTTG ATGATCTTGT CCACCTGTAT CATCTTGATC CTGAGGTTGC ATTTTTAATA 
TACCGCCCAG TCATGAGACT CTTCAAGTGT GAGGGTAGTT TAGAGGTTTT CTGGCCCTTA 
GACAGCAATG AAACAGTAAC TACTACAAGT GCAAACCTGG AGCCTGAAGC TAGAGAATAT 
TCTGGCAGAG TGATTCTGGA TCTTGGCTCT CTCCATAAGC CTGTAATGTG GTCAGATCTT 
CTTGAGACTC TGAAAACCAT GTTGCCTTCA AAAGCCTGGA ATAGCCTCTC ACCTGATCTC 
TATGCCACTT TCTGGGGACT CACACTTTAT GATCTATATG TCCCTAGAAA CCGTTACGAG 
TCTGAAATTG CCAAGCAGCA AGCTGCTCTT AAAGCGCTGG AAGAGCTTTC TGATAATTCT 
AGTTCAGCAA TTACTAAAAG GAAGAAAGAG AAGGAAAGGA TTCAAGAATC TCTCGATCGT 
CTGACTAGTG AGCTCCATAA ACATGAAGAA AATGTTTCTT CTGTTCGTAG ACGACTATCT 
CGTGAGAAGG ACAAATGGCT GACTTCTTGT CCTGATACTT TGAAAATCAA CATGGAGTTC 
CTTCAGCGCT GTATTTTTCC CCGTTGTACT TTCAGCATGC CGGATGCTGT ATATTGTGCC 
ATGTTTGTAC ATACTCTTCA TTCACTTGGC ACACCATTTT TCAACACTGT AAACCACATC 
GATGTTCTGA TATGTAAAAC ATTACAACCT ATGATATGCT GCTGTACTGA ATATGAAGCA 
GGTAGACTTG GGAGGTTTTT GTATGAAACA CTCAAGATTG CTTACTACTG GAAGAGTGAT 
GAAACTATAT ATGAACGTGA GTGTGGAAAT ATGCCTGGAT TTGCTGTGTA TTATAGATTC 
CCAAACAGCC AACGGGTTAC ATATGGTCAG TTCATTAAGG TACACTGGAA ATGGAGTCAG 
AGAATGTCTC GGCTATTGAT TCAGTGTCTG GAATCATCTG AGTACATGGA AATTCGAAAT 
GCTCTTATTT TGTTGACAAA AATTTCCGGT GTTTTTCCAG TTACTAAAAG GAGTGGTATT 
AACCTTGAAA AACGAGTAAC TAGAATCAAA AGTGATGAAA GGGAGGATCT CAAGGTTTTG 
GCTACAGGTG TTGCTGCAGC TTTGGCTGCT AGAAAGCCTT CATGGGTTAC AGATGAAGAA 
TTTGGCATGG GATATCTTGA CATAAAGCCT CCATCTGTTG CATCAAAGTC TTTATCTGGA 
AATGTAGCAG CAGCACAAAA CAGTTCTGCC CTTAATGTTT CTCAAGGTGA ACCTGCTGAT 
GGAAGAGCAC TTGTAACTGG AAGCCAACAT GGAGATCCTG GGAATTCAAA CAGAGATCCG 
ATATCCAGAG CAAAACATGC TGATGGGAGG TCAGATAGAA CAGAAAATAT CTCACATTTG 
AAATCTGATC TAGGACATCA GAAATCAAAA GGTGGTTCAT CAACCAATGG GTCAAATGCT 
CAGTCAGCTG TTTCCTCGGC TGCTGTTCCT ATAGGTGCAT CAAGATCAGC AGAAAATCAG 
AAGGGGATGG ATGACTCCAC AAATAGAACA TTGGAGGATA GCACTGTCAG AGTTGCTGCA 
AAGAACTTGG CGGAGTCTGA GTTGAAAGTT TCCACAAAGC GGCCAGTGTC TAAAACACCA 
AAGCAAGATG TTGTGAAAGA TGACAATAAA TCTGGAAAAG GTGTTGGCCG AACTCTATCC 
TCATCCACAA GTGACAAAGA CATCCAGGTT CATTTATCAG AGGGAAGACA AGGGGGTGCT 
TCTAATGTTT CTTCTGTGCT CACATCGAAT GGTAATGCTC TTTCAGTGTC TGAAAAAGTC 
TCTACTATGG TTACAAGAAC TTCTGATTCT TATGGTGTAG AATCAAAGCC AGATAGTGGA 
GGTAACAAAC CTATGCTAAA AGATGAAGCA ACTGAAGTTG CTGATGTGCA GAAGCCACCT 
TCTCGACTTG TTCATTCTCC TAGGCATGAT AACTCTGTTG CTGCTTCAAA GTCTAGTGAT 
AAATTGCAAA AGAGAGCAAG TCCTGCTGAA GAACCTGACA GATTAAGTAA AAGGCAAAAA 
GGGGATGTCG AATTGAGAGA TTTAGAGGGT GAAGTTAAAT TCTCTGAAAG AGAGAGGTCC 
ACCGATACTC GATCTGCGGA TCTTGATAAA GTGGGAAATG ATGAACATAA TCTATATAGG 
AGCGTAGATA AACCTTTGGA TAGGTCAAAA GATAAAGGTA ATGATAGATA TGACAGGGAC 
CATAGGGAAA GATCAGAGCG TCCTGATAAA TCCCGTGGAG ATGATAGTTT AGCTGACAGA 
TCGAGAGATA AATCAATGGA AAGATATGGA AGAGAGCTTT CGGTTGAGAG AGGGCAGGAT 
AGGGTCGCTG ATAGGAGTTT TGACAGACTA GCTGACAAAG CCAAAGATGA CAGAAGCAAA 
TTACGTTACA ATGATACCTC TGCAGAAAAA TCTCAGGTTG ATGATCGTTT TCATGGGCAA 
AACTTGCCTC CGCCACCACC TTTACCTCCT CATATGGTTC CCCAATCAGT TACTTCTGGT 
AGAAGAGATG AAGATGCTGA CAGAAGGTTT GGAACTACCA GACATGTTCA GAGGCTTTCT 
CCTAGGCATG ATGAGAAAGA ACGAAGACGA TCAGAAGAAA ATTCCTTGGT TTCACAGGAT 
GATGCAAAAC GTAGAAAAGA GGATGATGTT CGTGAGAGAA AGCGAGAAGA GCGAGAGGGG 
CTGTCAATCA AGGTGGAAGA GAGGGAAAGA GAGAGGGAGA GGGAGAAAAC CAACCTTCCG 
AAGGAGGAAA TGGACTCCAG TGCAACTGCC AAGAGACGAA AACTTAAAAG AGATCATCTG 
CCGACAGGGG AAGCTGGTGA ATATTCTCCT GTTGCCCCAC CACCTCCACC CCTTGGTATT 
GGCATTTCAC ATTCATATGA TGGTAGGGAG AGGGGAGATA GGAAGGGGGC CATGAATCAG 
CGTGCAAGTT ACTTGGAGGA ACCTCTCATG AGGATTCATG GTAAAGATGT AGTTGGCAAG 
ATGGGCCGCC GTGATACTGA TCCTATGTAT GATCGGGAAT GGGATGAGGA CAAGAGGCAG 
AGAGCTGAAC AAAAGCGGAG GCATCGCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18104229 ( XM_024584133.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024439901.1 Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPa442342
Clone ID Related Accession (Same CDS sequence)	XM_024584133.1
Accession Version	XM_024584133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	THO complex subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037297.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGAAT CAAAGCCAGA TAGTGGAGGT AACAAACCTA TGCTAAAAGA TGAAGCAACT 
GAAGTTGCTG ATGTGCAGAA GCCACCTTCT CGACTTGTTC ATTCTCCTAG GCATGATAAC 
TCTGTTGCTG CTTCAAAGTC TAGTGATAAA TTGCAAAAGA GAGCAAGTCC TGCTGAAGAA 
CCTGACAGAT TAAGTAAAAG GCAAAAAGGG GATGTCGAAT TGAGAGATTT AGAGGGTGAA 
GTTAAATTCT CTGAAAGAGA GAGGTCCACC GATACTCGAT CTGCGGATCT TGATAAAGTG 
GGAAATGATG AACATAATCT ATATAGGAGC GTAGATAAAC CTTTGGATAG GTCAAAAGAT 
AAAGGTAATG ATAGATATGA CAGGGACCAT AGGGAAAGAT CAGAGCGTCC TGATAAATCC 
CGTGGAGATG ATAGTTTAGC TGACAGATCG AGAGATAAAT CAATGGAAAG ATATGGAAGA 
GAGCTTTCGG TTGAGAGAGG GCAGGATAGG GTCGCTGATA GGAGTTTTGA CAGACTAGCT 
GACAAAGCCA AAGATGACAG AAGCAAATTA CGTTACAATG ATACCTCTGC AGAAAAATCT 
CAGGTTGATG ATCGTTTTCA TGGGCAAAAC TTGCCTCCGC CACCACCTTT ACCTCCTCAT 
ATGGTTCCCC AATCAGTTAC TTCTGGTAGA AGAGATGAAG ATGCTGACAG AAGGTTTGGA 
ACTACCAGAC ATGTTCAGAG GCTTTCTCCT AGGCATGATG AGAAAGAACG AAGACGATCA 
GAAGAAAATT CCTTGGTTTC ACAGGATGAT GCAAAACGTA GAAAAGAGGA TGATGTTCGT 
GAGAGAAAGC GAGAAGAGCG AGAGGGGCTG TCAATCAAGG TGGAAGAGAG GGAAAGAGAG 
AGGGAGAGGG AGAAAACCAA CCTTCCGAAG GAGGAAATGG ACTCCAGTGC AACTGCCAAG 
AGACGAAAAC TTAAAAGAGA TCATCTGCCG ACAGGGGAAG CTGGTGAATA TTCTCCTGTT 
GCCCCACCAC CTCCACCCCT TGGTATTGGC ATTTCACATT CATATGATGG TAGGGAGAGG 
GGAGATAGGA AGGGGGCCAT GAATCAGCGT GCAAGTTACT TGGAGGAACC TCTCATGAGG 
ATTCATGGTA AAGATGTAGT TGGCAAGATG GGCCGCCGTG ATACTGATCC TATGTATGAT 
CGGGAATGGG ATGAGGACAA GAGGCAGAGA GCTGAACAAA AGCGGAGGCA TCGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024439901.1
CDS	165..5744
Translation	MSTTLPPMECLHVTEEFLLELKSGNRSFRLPHPVPILRFLYELSWTLVRGELPFQKCKAALDSVEFVDKMSAVGLGSNFADIITQMAQDLTMSGEYRSRLIKLAKWLVESALVPLRFFQERCEEEFLWEAEMIKIKAQDLKGKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCRGSEDTAENTSAATIGIIKSLIGHFDLDPNRVFDIVLECFELQPDSNVFLELIPIFPKSHASQILGFKFQYYQRMELNSPVPFGLFKLTALLVKEEFIDLDSICAHLLPKDDEAFEHYNTFSSKRLDAAYKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMETEAVAEQFSDLEKNQTLGLLTGFLSVDDWYHAHILFKRLSPLNPVAHTQICGGLFRLIEKTISSAYNIIRQPHVQNCGSPAVAGIDAMDVTSSSGHVSLIDLPKEFFQMLVTVGPYLYRDTLLLQKVCRVLRGYYMSALELVDSGDGALNGESLIPRNRVLRLHLREGRSMVEEALGACLLPSLQLVPANPAAGQEIWEVMSLLPYEVRYRLYGEWEKDDERNPLVLAARQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMITPVVDAFKYLTQLEYDILEYVVIERLAQGGRDKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGQGIELVLLQELVQQMANVQYTENLTEEQLDAMAGSETLRYQATSFGVTRNNKALFKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVINADAPYIKMVSEQFDRCHGTLLQYVEFLCGAVTPPSAYAQLIPSLDDLVHLYHLDPEVAFLIYRPVMRLFKCEGSLEVFWPLDSNETVTTTSANLEPEAREYSGRVILDLGSLHKPVMWSDLLETLKTMLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQQAALKALEELSDNSSSAITKRKKEKERIQESLDRLTSELHKHEENVSSVRRRLSREKDKWLTSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVHTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEAGRLGRFLYETLKIAYYWKSDETIYERECGNMPGFAVYYRFPNSQRVTYGQFIKVHWKWSQRMSRLLIQCLESSEYMEIRNALILLTKISGVFPVTKRSGINLEKRVTRIKSDEREDLKVLATGVAAALAARKPSWVTDEEFGMGYLDIKPPSVASKSLSGNVAAAQNSSALNVSQGEPADGRALVTGSQHGDPGNSNRDPISRAKHADGRSDRTENISHLKSDLGHQKSKGGSSTNGSNAQSAVSSAAVPIGASRSAENQKGMDDSTNRTLEDSTVRVAAKNLAESELKVSTKRPVSKTPKQDVVKDDNKSGKGVGRTLSSSTSDKDIQVHLSEGRQGGASNVSSVLTSNESKPDSGGNKPMLKDEATEVADVQKPPSRLVHSPRHDNSVAASKSSDKLQKRASPAEEPDRLSKRQKGDVELRDLEGEVKFSERERSTDTRSADLDKVGNDEHNLYRSVDKPLDRSKDKGNDRYDRDHRERSERPDKSRGDDSLADRSRDKSMERYGRELSVERGQDRVADRSFDRLADKAKDDRSKLRYNDTSAEKSQVDDRFHGQNLPPPPPLPPHMVPQSVTSGRRDEDADRRFGTTRHVQRLSPRHDEKERRRSEENSLVSQDDAKRRKEDDVRERKREEREGLSIKVEEREREREREKTNLPKEEMDSSATAKRRKLKRDHLPTGEAGEYSPVAPPPPPLGIGISHSYDGRERGDRKGAMNQRASYLEEPLMRIHGKDVVGKMGRRDTDPMYDREWDEDKRQRAEQKRRHRK

Target ORF information:

RefSeq Version	XM_024584133.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024584133.1

ORF Insert Sequence:

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGAAT CAAAGCCAGA TAGTGGAGGT AACAAACCTA TGCTAAAAGA TGAAGCAACT 
GAAGTTGCTG ATGTGCAGAA GCCACCTTCT CGACTTGTTC ATTCTCCTAG GCATGATAAC 
TCTGTTGCTG CTTCAAAGTC TAGTGATAAA TTGCAAAAGA GAGCAAGTCC TGCTGAAGAA 
CCTGACAGAT TAAGTAAAAG GCAAAAAGGG GATGTCGAAT TGAGAGATTT AGAGGGTGAA 
GTTAAATTCT CTGAAAGAGA GAGGTCCACC GATACTCGAT CTGCGGATCT TGATAAAGTG 
GGAAATGATG AACATAATCT ATATAGGAGC GTAGATAAAC CTTTGGATAG GTCAAAAGAT 
AAAGGTAATG ATAGATATGA CAGGGACCAT AGGGAAAGAT CAGAGCGTCC TGATAAATCC 
CGTGGAGATG ATAGTTTAGC TGACAGATCG AGAGATAAAT CAATGGAAAG ATATGGAAGA 
GAGCTTTCGG TTGAGAGAGG GCAGGATAGG GTCGCTGATA GGAGTTTTGA CAGACTAGCT 
GACAAAGCCA AAGATGACAG AAGCAAATTA CGTTACAATG ATACCTCTGC AGAAAAATCT 
CAGGTTGATG ATCGTTTTCA TGGGCAAAAC TTGCCTCCGC CACCACCTTT ACCTCCTCAT 
ATGGTTCCCC AATCAGTTAC TTCTGGTAGA AGAGATGAAG ATGCTGACAG AAGGTTTGGA 
ACTACCAGAC ATGTTCAGAG GCTTTCTCCT AGGCATGATG AGAAAGAACG AAGACGATCA 
GAAGAAAATT CCTTGGTTTC ACAGGATGAT GCAAAACGTA GAAAAGAGGA TGATGTTCGT 
GAGAGAAAGC GAGAAGAGCG AGAGGGGCTG TCAATCAAGG TGGAAGAGAG GGAAAGAGAG 
AGGGAGAGGG AGAAAACCAA CCTTCCGAAG GAGGAAATGG ACTCCAGTGC AACTGCCAAG 
AGACGAAAAC TTAAAAGAGA TCATCTGCCG ACAGGGGAAG CTGGTGAATA TTCTCCTGTT 
GCCCCACCAC CTCCACCCCT TGGTATTGGC ATTTCACATT CATATGATGG TAGGGAGAGG 
GGAGATAGGA AGGGGGCCAT GAATCAGCGT GCAAGTTACT TGGAGGAACC TCTCATGAGG 
ATTCATGGTA AAGATGTAGT TGGCAAGATG GGCCGCCGTG ATACTGATCC TATGTATGAT 
CGGGAATGGG ATGAGGACAA GAGGCAGAGA GCTGAACAAA AGCGGAGGCA TCGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18104229 ( XM_024584134.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024439902.1 Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X6, mRNA.

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CloneID	OPa442343
Clone ID Related Accession (Same CDS sequence)	XM_024584134.1
Accession Version	XM_024584134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	THO complex subunit 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037297.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGATA GTGGAGGTAA CAAACCTATG CTAAAAGATG AAGCAACTGA AGTTGCTGAT 
GTGCAGAAGC CACCTTCTCG ACTTGTTCAT TCTCCTAGGC ATGATAACTC TGTTGCTGCT 
TCAAAGTCTA GTGATAAATT GCAAAAGAGA GCAAGTCCTG CTGAAGAACC TGACAGATTA 
AGTAAAAGGC AAAAAGGGGA TGTCGAATTG AGAGATTTAG AGGGTGAAGT TAAATTCTCT 
GAAAGAGAGA GGTCCACCGA TACTCGATCT GCGGATCTTG ATAAAGTGGG AAATGATGAA 
CATAATCTAT ATAGGAGCGT AGATAAACCT TTGGATAGGT CAAAAGATAA AGGTAATGAT 
AGATATGACA GGGACCATAG GGAAAGATCA GAGCGTCCTG ATAAATCCCG TGGAGATGAT 
AGTTTAGCTG ACAGATCGAG AGATAAATCA ATGGAAAGAT ATGGAAGAGA GCTTTCGGTT 
GAGAGAGGGC AGGATAGGGT CGCTGATAGG AGTTTTGACA GACTAGCTGA CAAAGCCAAA 
GATGACAGAA GCAAATTACG TTACAATGAT ACCTCTGCAG AAAAATCTCA GGTTGATGAT 
CGTTTTCATG GGCAAAACTT GCCTCCGCCA CCACCTTTAC CTCCTCATAT GGTTCCCCAA 
TCAGTTACTT CTGGTAGAAG AGATGAAGAT GCTGACAGAA GGTTTGGAAC TACCAGACAT 
GTTCAGAGGC TTTCTCCTAG GCATGATGAG AAAGAACGAA GACGATCAGA AGAAAATTCC 
TTGGTTTCAC AGGATGATGC AAAACGTAGA AAAGAGGATG ATGTTCGTGA GAGAAAGCGA 
GAAGAGCGAG AGGGGCTGTC AATCAAGGTG GAAGAGAGGG AAAGAGAGAG GGAGAGGGAG 
AAAACCAACC TTCCGAAGGA GGAAATGGAC TCCAGTGCAA CTGCCAAGAG ACGAAAACTT 
AAAAGAGATC ATCTGCCGAC AGGGGAAGCT GGTGAATATT CTCCTGTTGC CCCACCACCT 
CCACCCCTTG GTATTGGCAT TTCACATTCA TATGATGGTA GGGAGAGGGG AGATAGGAAG 
GGGGCCATGA ATCAGCGTGC AAGTTACTTG GAGGAACCTC TCATGAGGAT TCATGGTAAA 
GATGTAGTTG GCAAGATGGG CCGCCGTGAT ACTGATCCTA TGTATGATCG GGAATGGGAT 
GAGGACAAGA GGCAGAGAGC TGAACAAAAG CGGAGGCATC GCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024439902.1
CDS	165..5732
Translation	MSTTLPPMECLHVTEEFLLELKSGNRSFRLPHPVPILRFLYELSWTLVRGELPFQKCKAALDSVEFVDKMSAVGLGSNFADIITQMAQDLTMSGEYRSRLIKLAKWLVESALVPLRFFQERCEEEFLWEAEMIKIKAQDLKGKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCRGSEDTAENTSAATIGIIKSLIGHFDLDPNRVFDIVLECFELQPDSNVFLELIPIFPKSHASQILGFKFQYYQRMELNSPVPFGLFKLTALLVKEEFIDLDSICAHLLPKDDEAFEHYNTFSSKRLDAAYKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMETEAVAEQFSDLEKNQTLGLLTGFLSVDDWYHAHILFKRLSPLNPVAHTQICGGLFRLIEKTISSAYNIIRQPHVQNCGSPAVAGIDAMDVTSSSGHVSLIDLPKEFFQMLVTVGPYLYRDTLLLQKVCRVLRGYYMSALELVDSGDGALNGESLIPRNRVLRLHLREGRSMVEEALGACLLPSLQLVPANPAAGQEIWEVMSLLPYEVRYRLYGEWEKDDERNPLVLAARQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMITPVVDAFKYLTQLEYDILEYVVIERLAQGGRDKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGQGIELVLLQELVQQMANVQYTENLTEEQLDAMAGSETLRYQATSFGVTRNNKALFKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVINADAPYIKMVSEQFDRCHGTLLQYVEFLCGAVTPPSAYAQLIPSLDDLVHLYHLDPEVAFLIYRPVMRLFKCEGSLEVFWPLDSNETVTTTSANLEPEAREYSGRVILDLGSLHKPVMWSDLLETLKTMLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQQAALKALEELSDNSSSAITKRKKEKERIQESLDRLTSELHKHEENVSSVRRRLSREKDKWLTSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVHTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEAGRLGRFLYETLKIAYYWKSDETIYERECGNMPGFAVYYRFPNSQRVTYGQFIKVHWKWSQRMSRLLIQCLESSEYMEIRNALILLTKISGVFPVTKRSGINLEKRVTRIKSDEREDLKVLATGVAAALAARKPSWVTDEEFGMGYLDIKPPSVASKSLSGNVAAAQNSSALNVSQGEPADGRALVTGSQHGDPGNSNRDPISRAKHADGRSDRTENISHLKSDLGHQKSKGGSSTNGSNAQSAVSSAAVPIGASRSAENQKGMDDSTNRTLEDSTVRVAAKNLAESELKVSTKRPVSKTPKQDVVKDDNKSGKGVGRTLSSSTSDKDIQVHLSEGRQGGASNVSSVLTSNDSGGNKPMLKDEATEVADVQKPPSRLVHSPRHDNSVAASKSSDKLQKRASPAEEPDRLSKRQKGDVELRDLEGEVKFSERERSTDTRSADLDKVGNDEHNLYRSVDKPLDRSKDKGNDRYDRDHRERSERPDKSRGDDSLADRSRDKSMERYGRELSVERGQDRVADRSFDRLADKAKDDRSKLRYNDTSAEKSQVDDRFHGQNLPPPPPLPPHMVPQSVTSGRRDEDADRRFGTTRHVQRLSPRHDEKERRRSEENSLVSQDDAKRRKEDDVRERKREEREGLSIKVEEREREREREKTNLPKEEMDSSATAKRRKLKRDHLPTGEAGEYSPVAPPPPPLGIGISHSYDGRERGDRKGAMNQRASYLEEPLMRIHGKDVVGKMGRRDTDPMYDREWDEDKRQRAEQKRRHRK

Target ORF information:

RefSeq Version	XM_024584134.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa THO complex subunit 2 (LOC18104229), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024584134.1

ORF Insert Sequence:

ATGTCAACAA CTCTCCCTCC CATGGAGTGT TTACACGTAA CTGAAGAATT TCTCCTCGAA 
TTAAAGAGCG GAAACCGTAG CTTCCGTCTC CCTCACCCTG TACCTATTCT TCGATTTCTC 
TACGAGCTAT CCTGGACCTT GGTTCGAGGA GAATTGCCGT TTCAGAAGTG CAAAGCTGCT 
TTGGATTCTG TGGAGTTTGT TGATAAAATG TCTGCTGTAG GGTTGGGTTC GAATTTCGCT 
GATATCATTA CTCAAATGGC TCAAGATCTT ACAATGTCTG GAGAGTACCG ATCTCGTCTC 
ATAAAGCTGG CAAAATGGCT AGTTGAATCT GCGTTGGTCC CATTGAGGTT TTTCCAAGAG 
CGTTGTGAGG AGGAATTTTT ATGGGAAGCT GAAATGATAA AGATAAAGGC TCAAGACTTG 
AAGGGCAAAG AGGTCAGGGT AAATACTCGT CTTCTTTATC AGCAAACAAA ATTTAACCTT 
CTCCGTGAAG AGAGTGAGGG CTATGCCAAA CTGGTTACTC TTCTCTGTCG AGGTTCTGAA 
GATACAGCTG AAAATACATC AGCAGCGACA ATAGGCATCA TTAAGTCATT GATCGGTCAT 
TTTGATCTGG ACCCAAATCG TGTTTTCGAC ATTGTTTTAG AGTGTTTTGA ACTTCAGCCT 
GATAGCAATG TCTTTTTGGA ACTGATTCCC ATATTTCCTA AGTCGCATGC ATCACAAATT 
CTTGGTTTTA AGTTTCAGTA TTATCAGCGA ATGGAATTAA ATAGCCCTGT ACCATTTGGG 
CTCTTTAAGC TTACAGCCTT ACTAGTGAAG GAAGAGTTCA TTGATCTTGA TAGTATATGT 
GCACATTTAC TCCCTAAGGA TGATGAGGCT TTTGAGCATT ATAACACGTT TTCTTCCAAA 
CGATTGGATG CGGCCTACAA AATTGGAAAA ATAAATCTGG CTGCTACTGG AAAGGACCTC 
ATGGATGATG AGAAGCAAGG AGATGTGACA GTAGATCTTT TTGCTGCCTT AGACATGGAA 
ACTGAGGCAG TAGCTGAACA ATTCTCTGAT CTTGAAAAAA ATCAAACTTT GGGCTTGCTG 
ACTGGTTTCC TTTCTGTGGA TGACTGGTAT CATGCCCATA TACTTTTCAA ACGTCTCTCT 
CCTCTAAATC CTGTTGCACA TACTCAAATA TGTGGTGGCT TGTTTAGGCT CATTGAAAAG 
ACGATCTCAT CAGCATATAA TATTATTCGT CAACCACATG TTCAAAATTG TGGGTCTCCA 
GCAGTTGCTG GTATTGATGC CATGGATGTG ACAAGTTCTT CAGGCCATGT CTCTTTAATT 
GATCTTCCCA AAGAATTTTT TCAGATGCTT GTAACAGTTG GACCTTATCT TTACCGTGAT 
ACGTTATTGC TGCAGAAGGT TTGTAGAGTG TTAAGAGGTT ACTATATGTC TGCACTTGAG 
CTTGTAGATA GTGGTGATGG AGCTCTAAAT GGTGAATCTC TTATACCTAG AAATCGAGTT 
CTTCGTTTGC ACCTAAGGGA AGGTAGGTCA ATGGTTGAGG AAGCCCTGGG AGCATGTTTA 
CTTCCTTCTT TACAGTTGGT ACCTGCAAAT CCAGCAGCTG GGCAGGAGAT CTGGGAAGTA 
ATGAGTCTCC TTCCATATGA GGTGCGATAT CGTTTATATG GTGAATGGGA AAAAGATGAT 
GAGCGGAATC CATTGGTTTT GGCTGCAAGG CAAACAGCCA AGTTGGATAC TAGAAGGATA 
TTGAAAAGGC TTGCGAAGGA AAATTTGAAG CAGCTGGGTA GGATGGTTGC AAAATTAGCG 
CATGCTAACC CGATGACTGT CCTTCGAACT ATTGTTCATC AGATTGAGGC ATACAGGGAT 
ATGATCACTC CTGTTGTGGA TGCTTTCAAG TATTTGACAC AGCTTGAGTA TGATATCTTG 
GAATATGTTG TAATTGAGCG GTTAGCACAA GGTGGTCGTG ATAAGCTCAA GGATGATGGC 
CTTAATTTGT CAGACTGGCT TCAATCACTG GCTTCATTCT GGGGTCACCT GTGTAAGAAG 
TACCCATCTA TGGAATTGAG AGGCCTTTTC CAGTATTTAG TGAACCAGTT GAAAAAGGGC 
CAGGGGATTG AGCTTGTTCT TCTGCAGGAG CTTGTTCAAC AAATGGCAAA TGTTCAATAC 
ACAGAAAACT TAACAGAGGA ACAGTTGGAT GCCATGGCAG GGAGTGAGAC ACTTCGGTAT 
CAAGCTACTT CTTTTGGAGT AACACGGAAT AACAAGGCGT TGTTCAAATC TACTAACAGG 
CTTCGAGACT CCTTGCTTCC TAAGGATGAA CCAAAGCTAG CGATCCCCCT TTTGTTACTT 
ATTGCTCAAC ATCGATCTGT GGTTGTCATA AATGCAGATG CTCCTTACAT TAAAATGGTC 
AGTGAGCAGT TTGATAGGTG TCATGGAACT CTTCTTCAAT ATGTGGAATT CCTCTGTGGT 
GCTGTGACAC CACCCTCTGC ATATGCTCAG TTAATTCCCT CTCTTGATGA TCTTGTCCAC 
CTGTATCATC TTGATCCTGA GGTTGCATTT TTAATATACC GCCCAGTCAT GAGACTCTTC 
AAGTGTGAGG GTAGTTTAGA GGTTTTCTGG CCCTTAGACA GCAATGAAAC AGTAACTACT 
ACAAGTGCAA ACCTGGAGCC TGAAGCTAGA GAATATTCTG GCAGAGTGAT TCTGGATCTT 
GGCTCTCTCC ATAAGCCTGT AATGTGGTCA GATCTTCTTG AGACTCTGAA AACCATGTTG 
CCTTCAAAAG CCTGGAATAG CCTCTCACCT GATCTCTATG CCACTTTCTG GGGACTCACA 
CTTTATGATC TATATGTCCC TAGAAACCGT TACGAGTCTG AAATTGCCAA GCAGCAAGCT 
GCTCTTAAAG CGCTGGAAGA GCTTTCTGAT AATTCTAGTT CAGCAATTAC TAAAAGGAAG 
AAAGAGAAGG AAAGGATTCA AGAATCTCTC GATCGTCTGA CTAGTGAGCT CCATAAACAT 
GAAGAAAATG TTTCTTCTGT TCGTAGACGA CTATCTCGTG AGAAGGACAA ATGGCTGACT 
TCTTGTCCTG ATACTTTGAA AATCAACATG GAGTTCCTTC AGCGCTGTAT TTTTCCCCGT 
TGTACTTTCA GCATGCCGGA TGCTGTATAT TGTGCCATGT TTGTACATAC TCTTCATTCA 
CTTGGCACAC CATTTTTCAA CACTGTAAAC CACATCGATG TTCTGATATG TAAAACATTA 
CAACCTATGA TATGCTGCTG TACTGAATAT GAAGCAGGTA GACTTGGGAG GTTTTTGTAT 
GAAACACTCA AGATTGCTTA CTACTGGAAG AGTGATGAAA CTATATATGA ACGTGAGTGT 
GGAAATATGC CTGGATTTGC TGTGTATTAT AGATTCCCAA ACAGCCAACG GGTTACATAT 
GGTCAGTTCA TTAAGGTACA CTGGAAATGG AGTCAGAGAA TGTCTCGGCT ATTGATTCAG 
TGTCTGGAAT CATCTGAGTA CATGGAAATT CGAAATGCTC TTATTTTGTT GACAAAAATT 
TCCGGTGTTT TTCCAGTTAC TAAAAGGAGT GGTATTAACC TTGAAAAACG AGTAACTAGA 
ATCAAAAGTG ATGAAAGGGA GGATCTCAAG GTTTTGGCTA CAGGTGTTGC TGCAGCTTTG 
GCTGCTAGAA AGCCTTCATG GGTTACAGAT GAAGAATTTG GCATGGGATA TCTTGACATA 
AAGCCTCCAT CTGTTGCATC AAAGTCTTTA TCTGGAAATG TAGCAGCAGC ACAAAACAGT 
TCTGCCCTTA ATGTTTCTCA AGGTGAACCT GCTGATGGAA GAGCACTTGT AACTGGAAGC 
CAACATGGAG ATCCTGGGAA TTCAAACAGA GATCCGATAT CCAGAGCAAA ACATGCTGAT 
GGGAGGTCAG ATAGAACAGA AAATATCTCA CATTTGAAAT CTGATCTAGG ACATCAGAAA 
TCAAAAGGTG GTTCATCAAC CAATGGGTCA AATGCTCAGT CAGCTGTTTC CTCGGCTGCT 
GTTCCTATAG GTGCATCAAG ATCAGCAGAA AATCAGAAGG GGATGGATGA CTCCACAAAT 
AGAACATTGG AGGATAGCAC TGTCAGAGTT GCTGCAAAGA ACTTGGCGGA GTCTGAGTTG 
AAAGTTTCCA CAAAGCGGCC AGTGTCTAAA ACACCAAAGC AAGATGTTGT GAAAGATGAC 
AATAAATCTG GAAAAGGTGT TGGCCGAACT CTATCCTCAT CCACAAGTGA CAAAGACATC 
CAGGTTCATT TATCAGAGGG AAGACAAGGG GGTGCTTCTA ATGTTTCTTC TGTGCTCACA 
TCGAATGATA GTGGAGGTAA CAAACCTATG CTAAAAGATG AAGCAACTGA AGTTGCTGAT 
GTGCAGAAGC CACCTTCTCG ACTTGTTCAT TCTCCTAGGC ATGATAACTC TGTTGCTGCT 
TCAAAGTCTA GTGATAAATT GCAAAAGAGA GCAAGTCCTG CTGAAGAACC TGACAGATTA 
AGTAAAAGGC AAAAAGGGGA TGTCGAATTG AGAGATTTAG AGGGTGAAGT TAAATTCTCT 
GAAAGAGAGA GGTCCACCGA TACTCGATCT GCGGATCTTG ATAAAGTGGG AAATGATGAA 
CATAATCTAT ATAGGAGCGT AGATAAACCT TTGGATAGGT CAAAAGATAA AGGTAATGAT 
AGATATGACA GGGACCATAG GGAAAGATCA GAGCGTCCTG ATAAATCCCG TGGAGATGAT 
AGTTTAGCTG ACAGATCGAG AGATAAATCA ATGGAAAGAT ATGGAAGAGA GCTTTCGGTT 
GAGAGAGGGC AGGATAGGGT CGCTGATAGG AGTTTTGACA GACTAGCTGA CAAAGCCAAA 
GATGACAGAA GCAAATTACG TTACAATGAT ACCTCTGCAG AAAAATCTCA GGTTGATGAT 
CGTTTTCATG GGCAAAACTT GCCTCCGCCA CCACCTTTAC CTCCTCATAT GGTTCCCCAA 
TCAGTTACTT CTGGTAGAAG AGATGAAGAT GCTGACAGAA GGTTTGGAAC TACCAGACAT 
GTTCAGAGGC TTTCTCCTAG GCATGATGAG AAAGAACGAA GACGATCAGA AGAAAATTCC 
TTGGTTTCAC AGGATGATGC AAAACGTAGA AAAGAGGATG ATGTTCGTGA GAGAAAGCGA 
GAAGAGCGAG AGGGGCTGTC AATCAAGGTG GAAGAGAGGG AAAGAGAGAG GGAGAGGGAG 
AAAACCAACC TTCCGAAGGA GGAAATGGAC TCCAGTGCAA CTGCCAAGAG ACGAAAACTT 
AAAAGAGATC ATCTGCCGAC AGGGGAAGCT GGTGAATATT CTCCTGTTGC CCCACCACCT 
CCACCCCTTG GTATTGGCAT TTCACATTCA TATGATGGTA GGGAGAGGGG AGATAGGAAG 
GGGGCCATGA ATCAGCGTGC AAGTTACTTG GAGGAACCTC TCATGAGGAT TCATGGTAAA 
GATGTAGTTG GCAAGATGGG CCGCCGTGAT ACTGATCCTA TGTATGATCG GGAATGGGAT 
GAGGACAAGA GGCAGAGAGC TGAACAAAAG CGGAGGCATC GCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC18104229 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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