SMCHD1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCHD1
Entrez Gene ID	102990901
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007126742.1	XP_007126804.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
XM_007126742.2	XP_007126804.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
XM_007126744.1	XP_007126806.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
XM_007126742.3	XP_007126804.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1
XM_007126743.1	XP_007126805.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
XM_007126744.2	XP_007126806.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
XM_007126743.2	XP_007126805.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SMCHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh124538	XM_007126742.3 Latest version!	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh124539	XM_007126743.2 Latest version!	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh124540	XM_007126744.2 Latest version!	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OPh124538	XM_007126742.1	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh124538	XM_007126742.2	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh124540	XM_007126744.1	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh124539	XM_007126743.1	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCHD1 ( XM_007126742.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126804.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

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CloneID	OPh124538
Clone ID Related Accession (Same CDS sequence)	XM_007126742.1 , XM_007126742.2 , XM_007126742.3
Accession Version	XM_007126742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006724189.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126804.1
CDS	118..6111
Translation	MAAAGGGGPSVGTGEGGGDDGYRTVYLFDRREKESELGDRALQVTERSDFAAFRASVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGVRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126742.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126742.1

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126742.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126804.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

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CloneID	OPh124538
Clone ID Related Accession (Same CDS sequence)	XM_007126742.1 , XM_007126742.2 , XM_007126742.3
Accession Version	XM_007126742.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873555.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007126742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126804.1
CDS	179..6172
Translation	MAAAGGGGPSVGTGEGGGDDGYRTVYLFDRREKESELGDRALQVTERSDFAAFRASVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGVRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126742.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126742.2

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126742.3 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126804.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

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CloneID	OPh124538
Clone ID Related Accession (Same CDS sequence)	XM_007126742.1 , XM_007126742.2 , XM_007126742.3
Accession Version	XM_007126742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041232.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007126742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126804.1
CDS	332..6325
Translation	MAAAGGGGPSVGTGEGGGDDGYRTVYLFDRREKESELGDRALQVTERSDFAAFRASVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGVRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126742.3
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126742.3

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAACTGA CCTTTATGGA TCTTGAGCTA AAATTGAAAG ATAAGAACAC TCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TTTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACTTATTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTCGTCAAGA CAATCAAGAC ACTTCCTCTC 
TTTTATGGAA GCATTGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAAGCAC TGTATGATGA AATAAAAACT 
GTGCCAATTG CAAAGCTGGA TAGGACAGTT GCTGAGAAAG CTGTGAAAAA ATATGTAGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGACGAATTA 
TTGCCAAATG AGATTAGGCC TGCTGGAACC CCCATTGGTG CGTTAAGAAT TGAAATATTG 
AATAAAAAGG GGGAAGCAAT GCAAAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCCTGGTTG AGCTCAAAGT TATACTACAC TCTCCAAGTG GAAATAAAGA AATCATTTCA 
CATATTAGTC AACATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACATT GAAATTACAA GTTGTACTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCACTACC ATCTAAAGCA ATTAAATTTT CTGTCAAAGA GGGTAAACCA 
GAGAAATTTT CATTTGGTCT TTTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATC 
CCTCTGGAGT TTCAGGATGA ATTTGGTCAT ACTAGTCAAC TATCAACTGA TATTCAGCCA 
GTTTTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAATGG ACCAAATTGT 
GTCATTCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTTCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTG 
CCAGGTCCCC CTCATCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATAT AACAGCACAA 
CCAAAATTGA TTGTTCATTG TAAGTTTTCA GGTGCTCCAA ACCTTCCAGT CTACGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTG TCAGGACCAG CAATTCAAGT TCAGAATATC 
AAAAAAGACC AGATGCTTAA AGCAAAAATT GAAATACCTA GTTGTAAAGA TGTGGCACCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATATTCAGT 
GTAGAAGGAC AAAAGGCAAT CCAGATCAAA CATCAGGATG AGGTTAATTG GATAGCAGGA 
GATATTATGC ATAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAGCAC 
TTACTACAGG GTCTGCTTCC TGATGTACAA GTACCAACAT CTGTAAAAGA TATGCGCTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAATTAAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAGTATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TCTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAAC 
TCACATTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTGAG AGGCATCAAA 
TTTATTCCAG GTCCCCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGGCCA CCAGCTAAAC TTCTCCTTGT AGACTGGCCA 
GAGTTAAAGG AGTCCATTCC AGTGATTAAC GGAAGAGAAT TACAGAATCC TCTCATTGTT 
CAACTTTGTG ATCAATGGGA TAATCCAGCA CTTGTGCCAC AGGTTAAAAT AAGCCTTATA 
AAAGCTAACA ATTTAAAGCT CACTCCTTCA AACCAACAGC ATAAAACCGA TGAAAGGGGC 
AGGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAACATAT TATGCAGGTT 
AAAGCCATTT ATAACAAGAA TACCATAGAA GGACCCATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGGGGTATTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TTCCATGAAA ATGTGGAAAC TGTCAAACAG TGGGAACCGA 
CCACCAGCAA ATGCAGAAAC ATTTAGTTGT AATAAAATAA AGGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATC CTTAACAGTG AGCAGATTAT AGTGGATGTT 
CTACCTAATC AACCTGTGAA GTTAGTTCCT GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTG GTCAAAGATT TATATCTTCG TATCACGGAT 
GACTACAACA ATCATACTGG AATTGATTTG GTTGGCACTG TAATGGCTAC CATCAAAGGT 
TCTAATGAGG AAGATGACAC CCCACTCTTT ATTGGGAAAG TTAGAATACT TGAATTTCCC 
TTTGTGAAAG GTTCAGCTGA AATCACGAGT CTAGTTCTGG CAGAAAATAG TCCTGGAAAG 
GATAGCACTG AGTATTTTAT TGTGTTTGAG CCTCGGCTAC CAGGCTTATC AAGAACATTA 
GAATCATATA TCCTACCATT TATGTTTTAC AATGATGTTA AGAAGCAGCA ACAAATGGCA 
GCACTTACAA AAGAAAAGGA CCAATTATCT AAGTCTATTA TAATGTATAG AAGTTTATTT 
GATGCCAGCA AACAGCTTCT TGATGAGATG AAGTGCCAAG TTGAAGAAGC AAAATTAAAA 
GAGGCCCAGT TGCGAAATGA ACTAAAAACA CACAATATTG ACATTCCTAC AACACAACAG 
ATGCCACACA TTGAAGCACT TTTGAAGAGA AAGATATCAG AACAGGAAGA ACTAAAGAAA 
AAACCCAGAA GATCATGTAC TCTTCCAAAC TATACTAAAG GCAGTGGAGA TGTTTTGGGA 
AAGATTGCAC ACCTGGCACA AATTGAAGAT GATAGAGCCG CAATGGTTAT TTCTTGGCAC 
CTGGCAAGTG ACATGGACTG TGTGGTCACC CTGACCACTG ATGCTGCACG TCGTATCTAT 
GACGAAACCC AAGGTCGTCA GCAGGTGTTG CCCCTTGATT CTATTTATAA GAAGACTCTT 
CCAGATTGGA AAAGACCTCT GCCTCATTAC CGAAATGGAA AATTGTATTT TAAACCCATT 
GGAGATCCCA TCTTTGCCCG AGATTTGTTA ACATTTCCAG ATAACGTAGA ACATTGTGAA 
ACAGTATTTG GTATGCTGTT AGGAGACACC ATTATTTTGG ATAATTTGGA TGCTGCCAAT 
CATTATAGAA AAGAGGTTGT TAAAATTACA CACTGTCCTA CACTGCTGAC CAGAGATGGA 
GATCGCATTC GAAGTAATGG AAAGTTTGGG GGCCTTCAGA ATAAAGCCCC TCCAATGGAT 
AAACTTCGGG GAATGGTCTT TGGAGCTCCA GTACCAAAAC AGTGCCTGAT CTTAGGGGAA 
CAAATAGATC TTCTTCAGCA GTACCGCACT GCTGTGTGCA GGTTGGGCAG TGTGAACAAG 
GACCTTAACG GGCAGTTGGA ATACCTTCAC ACTCCAGATA TGAAGAAGAA AAAACAGGAA 
CTTGATGAAC AAGAGAAGAA TCTCAAACTG ATAGAGCAAA AACTAGGTAT GACTCCCACA 
CGTAAGTGTA ATGACTCACT GCGTCATCCA ATGACAGACT GTCCAGTTCC TCCTAAGAGA 
ATGAGAAGAG AAGCTACAAG ACAAAATAGG ATTATAACCA AAACAGATGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126743.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126805.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

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CloneID	OPh124539
Clone ID Related Accession (Same CDS sequence)	XM_007126743.1 , XM_007126743.2
Accession Version	XM_007126743.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006724189.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACTTA TTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTCGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATTGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAAG CACTGTATGA TGAAATAAAA ACTGTGCCAA TTGCAAAGCT GGATAGGACA 
GTTGCTGAGA AAGCTGTGAA AAAATATGTA GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGACGAA TTATTGCCAA ATGAGATTAG GCCTGCTGGA 
ACCCCCATTG GTGCGTTAAG AATTGAAATA TTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCCTGG TTGAGCTCAA AGTTATACTA 
CACTCTCCAA GTGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAC TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCATCTAAA 
GCAATTAAAT TTTCTGTCAA AGAGGGTAAA CCAGAGAAAT TTTCATTTGG TCTTTTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AACTATCAAC TGATATTCAG CCAGTTTTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA TGGACCAAAT TGTGTCATTC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTTCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTGCCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TATAACAGCA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TCAGGTGCTC CAAACCTTCC AGTCTACGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTGTCAGGAC CAGCAATTCA AGTTCAGAAT ATCAAAAAAG ACCAGATGCT TAAAGCAAAA 
ATTGAAATAC CTAGTTGTAA AGATGTGGCA CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGAGATATTA TGCATAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAG CACTTACTAC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTAAA AGATATGCGC TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTCTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AACTCACATT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT GAGAGGCATC AAATTTATTC CAGGTCCCCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGG 
CCACCAGCTA AACTTCTCCT TGTAGACTGG CCAGAGTTAA AGGAGTCCAT TCCAGTGATT 
AACGGAAGAG AATTACAGAA TCCTCTCATT GTTCAACTTT GTGATCAATG GGATAATCCA 
GCACTTGTGC CACAGGTTAA AATAAGCCTT ATAAAAGCTA ACAATTTAAA GCTCACTCCT 
TCAAACCAAC AGCATAAAAC CGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATTATGCAG GTTAAAGCCA TTTATAACAA GAATACCATA 
GAAGGACCCA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTTCCATG 
AAAATGTGGA AACTGTCAAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAGGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATCCTTAACA GTGAGCAGAT TATAGTGGAT GTTCTACCTA ATCAACCTGT GAAGTTAGTT 
CCTGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTGGTCAAAG ATTTATATCT TCGTATCACG GATGACTACA ACAATCATAC TGGAATTGAT 
TTGGTTGGCA CTGTAATGGC TACCATCAAA GGTTCTAATG AGGAAGATGA CACCCCACTC 
TTTATTGGGA AAGTTAGAAT ACTTGAATTT CCCTTTGTGA AAGGTTCAGC TGAAATCACG 
AGTCTAGTTC TGGCAGAAAA TAGTCCTGGA AAGGATAGCA CTGAGTATTT TATTGTGTTT 
GAGCCTCGGC TACCAGGCTT ATCAAGAACA TTAGAATCAT ATATCCTACC ATTTATGTTT 
TACAATGATG TTAAGAAGCA GCAACAAATG GCAGCACTTA CAAAAGAAAA GGACCAATTA 
TCTAAGTCTA TTATAATGTA TAGAAGTTTA TTTGATGCCA GCAAACAGCT TCTTGATGAG 
ATGAAGTGCC AAGTTGAAGA AGCAAAATTA AAAGAGGCCC AGTTGCGAAA TGAACTAAAA 
ACACACAATA TTGACATTCC TACAACACAA CAGATGCCAC ACATTGAAGC ACTTTTGAAG 
AGAAAGATAT CAGAACAGGA AGAACTAAAG AAAAAACCCA GAAGATCATG TACTCTTCCA 
AACTATACTA AAGGCAGTGG AGATGTTTTG GGAAAGATTG CACACCTGGC ACAAATTGAA 
GATGATAGAG CCGCAATGGT TATTTCTTGG CACCTGGCAA GTGACATGGA CTGTGTGGTC 
ACCCTGACCA CTGATGCTGC ACGTCGTATC TATGACGAAA CCCAAGGTCG TCAGCAGGTG 
TTGCCCCTTG ATTCTATTTA TAAGAAGACT CTTCCAGATT GGAAAAGACC TCTGCCTCAT 
TACCGAAATG GAAAATTGTA TTTTAAACCC ATTGGAGATC CCATCTTTGC CCGAGATTTG 
TTAACATTTC CAGATAACGT AGAACATTGT GAAACAGTAT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAATTT GGATGCTGCC AATCATTATA GAAAAGAGGT TGTTAAAATT 
ACACACTGTC CTACACTGCT GACCAGAGAT GGAGATCGCA TTCGAAGTAA TGGAAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCTCCAATG GATAAACTTC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AACAGTGCCT GATCTTAGGG GAACAAATAG ATCTTCTTCA GCAGTACCGC 
ACTGCTGTGT GCAGGTTGGG CAGTGTGAAC AAGGACCTTA ACGGGCAGTT GGAATACCTT 
CACACTCCAG ATATGAAGAA GAAAAAACAG GAACTTGATG AACAAGAGAA GAATCTCAAA 
CTGATAGAGC AAAAACTAGG TATGACTCCC ACACGTAAGT GTAATGACTC ACTGCGTCAT 
CCAATGACAG ACTGTCCAGT TCCTCCTAAG AGAATGAGAA GAGAAGCTAC AAGACAAAAT 
AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126805.1
CDS	118..6024
Translation	MAAAGGGGPSVGTGEGGGDDGYRTVYLFDRREKESELGDRALQVTERSDFAAFRASVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGVRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126743.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126743.1

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACTTA TTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTCGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATTGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAAG CACTGTATGA TGAAATAAAA ACTGTGCCAA TTGCAAAGCT GGATAGGACA 
GTTGCTGAGA AAGCTGTGAA AAAATATGTA GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGACGAA TTATTGCCAA ATGAGATTAG GCCTGCTGGA 
ACCCCCATTG GTGCGTTAAG AATTGAAATA TTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCCTGG TTGAGCTCAA AGTTATACTA 
CACTCTCCAA GTGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAC TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCATCTAAA 
GCAATTAAAT TTTCTGTCAA AGAGGGTAAA CCAGAGAAAT TTTCATTTGG TCTTTTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AACTATCAAC TGATATTCAG CCAGTTTTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA TGGACCAAAT TGTGTCATTC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTTCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTGCCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TATAACAGCA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TCAGGTGCTC CAAACCTTCC AGTCTACGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTGTCAGGAC CAGCAATTCA AGTTCAGAAT ATCAAAAAAG ACCAGATGCT TAAAGCAAAA 
ATTGAAATAC CTAGTTGTAA AGATGTGGCA CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGAGATATTA TGCATAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAG CACTTACTAC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTAAA AGATATGCGC TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTCTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AACTCACATT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT GAGAGGCATC AAATTTATTC CAGGTCCCCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGG 
CCACCAGCTA AACTTCTCCT TGTAGACTGG CCAGAGTTAA AGGAGTCCAT TCCAGTGATT 
AACGGAAGAG AATTACAGAA TCCTCTCATT GTTCAACTTT GTGATCAATG GGATAATCCA 
GCACTTGTGC CACAGGTTAA AATAAGCCTT ATAAAAGCTA ACAATTTAAA GCTCACTCCT 
TCAAACCAAC AGCATAAAAC CGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATTATGCAG GTTAAAGCCA TTTATAACAA GAATACCATA 
GAAGGACCCA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTTCCATG 
AAAATGTGGA AACTGTCAAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAGGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATCCTTAACA GTGAGCAGAT TATAGTGGAT GTTCTACCTA ATCAACCTGT GAAGTTAGTT 
CCTGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTGGTCAAAG ATTTATATCT TCGTATCACG GATGACTACA ACAATCATAC TGGAATTGAT 
TTGGTTGGCA CTGTAATGGC TACCATCAAA GGTTCTAATG AGGAAGATGA CACCCCACTC 
TTTATTGGGA AAGTTAGAAT ACTTGAATTT CCCTTTGTGA AAGGTTCAGC TGAAATCACG 
AGTCTAGTTC TGGCAGAAAA TAGTCCTGGA AAGGATAGCA CTGAGTATTT TATTGTGTTT 
GAGCCTCGGC TACCAGGCTT ATCAAGAACA TTAGAATCAT ATATCCTACC ATTTATGTTT 
TACAATGATG TTAAGAAGCA GCAACAAATG GCAGCACTTA CAAAAGAAAA GGACCAATTA 
TCTAAGTCTA TTATAATGTA TAGAAGTTTA TTTGATGCCA GCAAACAGCT TCTTGATGAG 
ATGAAGTGCC AAGTTGAAGA AGCAAAATTA AAAGAGGCCC AGTTGCGAAA TGAACTAAAA 
ACACACAATA TTGACATTCC TACAACACAA CAGATGCCAC ACATTGAAGC ACTTTTGAAG 
AGAAAGATAT CAGAACAGGA AGAACTAAAG AAAAAACCCA GAAGATCATG TACTCTTCCA 
AACTATACTA AAGGCAGTGG AGATGTTTTG GGAAAGATTG CACACCTGGC ACAAATTGAA 
GATGATAGAG CCGCAATGGT TATTTCTTGG CACCTGGCAA GTGACATGGA CTGTGTGGTC 
ACCCTGACCA CTGATGCTGC ACGTCGTATC TATGACGAAA CCCAAGGTCG TCAGCAGGTG 
TTGCCCCTTG ATTCTATTTA TAAGAAGACT CTTCCAGATT GGAAAAGACC TCTGCCTCAT 
TACCGAAATG GAAAATTGTA TTTTAAACCC ATTGGAGATC CCATCTTTGC CCGAGATTTG 
TTAACATTTC CAGATAACGT AGAACATTGT GAAACAGTAT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAATTT GGATGCTGCC AATCATTATA GAAAAGAGGT TGTTAAAATT 
ACACACTGTC CTACACTGCT GACCAGAGAT GGAGATCGCA TTCGAAGTAA TGGAAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCTCCAATG GATAAACTTC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AACAGTGCCT GATCTTAGGG GAACAAATAG ATCTTCTTCA GCAGTACCGC 
ACTGCTGTGT GCAGGTTGGG CAGTGTGAAC AAGGACCTTA ACGGGCAGTT GGAATACCTT 
CACACTCCAG ATATGAAGAA GAAAAAACAG GAACTTGATG AACAAGAGAA GAATCTCAAA 
CTGATAGAGC AAAAACTAGG TATGACTCCC ACACGTAAGT GTAATGACTC ACTGCGTCAT 
CCAATGACAG ACTGTCCAGT TCCTCCTAAG AGAATGAGAA GAGAAGCTAC AAGACAAAAT 
AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126743.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126805.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

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CloneID	OPh124539
Clone ID Related Accession (Same CDS sequence)	XM_007126743.1 , XM_007126743.2
Accession Version	XM_007126743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873555.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007126743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACTTA TTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTCGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATTGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAAG CACTGTATGA TGAAATAAAA ACTGTGCCAA TTGCAAAGCT GGATAGGACA 
GTTGCTGAGA AAGCTGTGAA AAAATATGTA GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGACGAA TTATTGCCAA ATGAGATTAG GCCTGCTGGA 
ACCCCCATTG GTGCGTTAAG AATTGAAATA TTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCCTGG TTGAGCTCAA AGTTATACTA 
CACTCTCCAA GTGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAC TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCATCTAAA 
GCAATTAAAT TTTCTGTCAA AGAGGGTAAA CCAGAGAAAT TTTCATTTGG TCTTTTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AACTATCAAC TGATATTCAG CCAGTTTTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA TGGACCAAAT TGTGTCATTC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTTCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTGCCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TATAACAGCA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TCAGGTGCTC CAAACCTTCC AGTCTACGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTGTCAGGAC CAGCAATTCA AGTTCAGAAT ATCAAAAAAG ACCAGATGCT TAAAGCAAAA 
ATTGAAATAC CTAGTTGTAA AGATGTGGCA CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGAGATATTA TGCATAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAG CACTTACTAC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTAAA AGATATGCGC TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTCTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AACTCACATT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT GAGAGGCATC AAATTTATTC CAGGTCCCCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGG 
CCACCAGCTA AACTTCTCCT TGTAGACTGG CCAGAGTTAA AGGAGTCCAT TCCAGTGATT 
AACGGAAGAG AATTACAGAA TCCTCTCATT GTTCAACTTT GTGATCAATG GGATAATCCA 
GCACTTGTGC CACAGGTTAA AATAAGCCTT ATAAAAGCTA ACAATTTAAA GCTCACTCCT 
TCAAACCAAC AGCATAAAAC CGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATTATGCAG GTTAAAGCCA TTTATAACAA GAATACCATA 
GAAGGACCCA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTTCCATG 
AAAATGTGGA AACTGTCAAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAGGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATCCTTAACA GTGAGCAGAT TATAGTGGAT GTTCTACCTA ATCAACCTGT GAAGTTAGTT 
CCTGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTGGTCAAAG ATTTATATCT TCGTATCACG GATGACTACA ACAATCATAC TGGAATTGAT 
TTGGTTGGCA CTGTAATGGC TACCATCAAA GGTTCTAATG AGGAAGATGA CACCCCACTC 
TTTATTGGGA AAGTTAGAAT ACTTGAATTT CCCTTTGTGA AAGGTTCAGC TGAAATCACG 
AGTCTAGTTC TGGCAGAAAA TAGTCCTGGA AAGGATAGCA CTGAGTATTT TATTGTGTTT 
GAGCCTCGGC TACCAGGCTT ATCAAGAACA TTAGAATCAT ATATCCTACC ATTTATGTTT 
TACAATGATG TTAAGAAGCA GCAACAAATG GCAGCACTTA CAAAAGAAAA GGACCAATTA 
TCTAAGTCTA TTATAATGTA TAGAAGTTTA TTTGATGCCA GCAAACAGCT TCTTGATGAG 
ATGAAGTGCC AAGTTGAAGA AGCAAAATTA AAAGAGGCCC AGTTGCGAAA TGAACTAAAA 
ACACACAATA TTGACATTCC TACAACACAA CAGATGCCAC ACATTGAAGC ACTTTTGAAG 
AGAAAGATAT CAGAACAGGA AGAACTAAAG AAAAAACCCA GAAGATCATG TACTCTTCCA 
AACTATACTA AAGGCAGTGG AGATGTTTTG GGAAAGATTG CACACCTGGC ACAAATTGAA 
GATGATAGAG CCGCAATGGT TATTTCTTGG CACCTGGCAA GTGACATGGA CTGTGTGGTC 
ACCCTGACCA CTGATGCTGC ACGTCGTATC TATGACGAAA CCCAAGGTCG TCAGCAGGTG 
TTGCCCCTTG ATTCTATTTA TAAGAAGACT CTTCCAGATT GGAAAAGACC TCTGCCTCAT 
TACCGAAATG GAAAATTGTA TTTTAAACCC ATTGGAGATC CCATCTTTGC CCGAGATTTG 
TTAACATTTC CAGATAACGT AGAACATTGT GAAACAGTAT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAATTT GGATGCTGCC AATCATTATA GAAAAGAGGT TGTTAAAATT 
ACACACTGTC CTACACTGCT GACCAGAGAT GGAGATCGCA TTCGAAGTAA TGGAAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCTCCAATG GATAAACTTC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AACAGTGCCT GATCTTAGGG GAACAAATAG ATCTTCTTCA GCAGTACCGC 
ACTGCTGTGT GCAGGTTGGG CAGTGTGAAC AAGGACCTTA ACGGGCAGTT GGAATACCTT 
CACACTCCAG ATATGAAGAA GAAAAAACAG GAACTTGATG AACAAGAGAA GAATCTCAAA 
CTGATAGAGC AAAAACTAGG TATGACTCCC ACACGTAAGT GTAATGACTC ACTGCGTCAT 
CCAATGACAG ACTGTCCAGT TCCTCCTAAG AGAATGAGAA GAGAAGCTAC AAGACAAAAT 
AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126805.1
CDS	179..6085
Translation	MAAAGGGGPSVGTGEGGGDDGYRTVYLFDRREKESELGDRALQVTERSDFAAFRASVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGVRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126743.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126743.2

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACCG GAGAGGGCGG CGGAGACGAC 
GGCTACCGGA CGGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCGGAGCT CGGGGACCGG 
GCGCTGCAGG TTACGGAGCG CTCGGACTTC GCGGCGTTCC GCGCCTCAGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA AAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAATTACC 
TGTGATAATT TTGATGAAAC TGTTAAAGAT GGAGTCACTC TGTACCTGCT ACAGTCAGTC 
AATCAGTTAC TACTAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
TTAGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTAATTGA TAATTCATTG TCTGCCACTT CTCGTAACAC TGGTGTTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCAG GATATGTTCG ACCACTACCA 
GTGCCACGCA GTTTAAATAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAATCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA GGATGTTCAT 
GAACTTGTAC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAATAAAGA GGCGATATAT 
AGCGGATATA TTAGAAACAG AAAGCCGTCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AAGGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGGG TACAACCAGA ACACATACAG TACTTGAAAA ATTATTTCCT TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGCC CAAAAGGGAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGACAC CTAAGATTAT CAACCTAAGG GAGATACAAG ATGACATGCA GACGTTGTAT 
ATAAATACAG CAGCCGATAG TTTTGAGTTC AAATCTCATG TTGAAGGAGA TGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTTTTA TATGATAGAG AAACGTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ACTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGACC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACATCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCGATT 
GAGTGCTACA ATAGGATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACTTA TTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTCGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATTGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAAG CACTGTATGA TGAAATAAAA ACTGTGCCAA TTGCAAAGCT GGATAGGACA 
GTTGCTGAGA AAGCTGTGAA AAAATATGTA GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGACGAA TTATTGCCAA ATGAGATTAG GCCTGCTGGA 
ACCCCCATTG GTGCGTTAAG AATTGAAATA TTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCCTGG TTGAGCTCAA AGTTATACTA 
CACTCTCCAA GTGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAC TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCATCTAAA 
GCAATTAAAT TTTCTGTCAA AGAGGGTAAA CCAGAGAAAT TTTCATTTGG TCTTTTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AACTATCAAC TGATATTCAG CCAGTTTTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA TGGACCAAAT TGTGTCATTC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTTCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTGCCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TATAACAGCA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TCAGGTGCTC CAAACCTTCC AGTCTACGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTGTCAGGAC CAGCAATTCA AGTTCAGAAT ATCAAAAAAG ACCAGATGCT TAAAGCAAAA 
ATTGAAATAC CTAGTTGTAA AGATGTGGCA CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGAGATATTA TGCATAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAG CACTTACTAC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTAAA AGATATGCGC TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTCTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AACTCACATT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT GAGAGGCATC AAATTTATTC CAGGTCCCCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGG 
CCACCAGCTA AACTTCTCCT TGTAGACTGG CCAGAGTTAA AGGAGTCCAT TCCAGTGATT 
AACGGAAGAG AATTACAGAA TCCTCTCATT GTTCAACTTT GTGATCAATG GGATAATCCA 
GCACTTGTGC CACAGGTTAA AATAAGCCTT ATAAAAGCTA ACAATTTAAA GCTCACTCCT 
TCAAACCAAC AGCATAAAAC CGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATTATGCAG GTTAAAGCCA TTTATAACAA GAATACCATA 
GAAGGACCCA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTTCCATG 
AAAATGTGGA AACTGTCAAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAGGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATCCTTAACA GTGAGCAGAT TATAGTGGAT GTTCTACCTA ATCAACCTGT GAAGTTAGTT 
CCTGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTGGTCAAAG ATTTATATCT TCGTATCACG GATGACTACA ACAATCATAC TGGAATTGAT 
TTGGTTGGCA CTGTAATGGC TACCATCAAA GGTTCTAATG AGGAAGATGA CACCCCACTC 
TTTATTGGGA AAGTTAGAAT ACTTGAATTT CCCTTTGTGA AAGGTTCAGC TGAAATCACG 
AGTCTAGTTC TGGCAGAAAA TAGTCCTGGA AAGGATAGCA CTGAGTATTT TATTGTGTTT 
GAGCCTCGGC TACCAGGCTT ATCAAGAACA TTAGAATCAT ATATCCTACC ATTTATGTTT 
TACAATGATG TTAAGAAGCA GCAACAAATG GCAGCACTTA CAAAAGAAAA GGACCAATTA 
TCTAAGTCTA TTATAATGTA TAGAAGTTTA TTTGATGCCA GCAAACAGCT TCTTGATGAG 
ATGAAGTGCC AAGTTGAAGA AGCAAAATTA AAAGAGGCCC AGTTGCGAAA TGAACTAAAA 
ACACACAATA TTGACATTCC TACAACACAA CAGATGCCAC ACATTGAAGC ACTTTTGAAG 
AGAAAGATAT CAGAACAGGA AGAACTAAAG AAAAAACCCA GAAGATCATG TACTCTTCCA 
AACTATACTA AAGGCAGTGG AGATGTTTTG GGAAAGATTG CACACCTGGC ACAAATTGAA 
GATGATAGAG CCGCAATGGT TATTTCTTGG CACCTGGCAA GTGACATGGA CTGTGTGGTC 
ACCCTGACCA CTGATGCTGC ACGTCGTATC TATGACGAAA CCCAAGGTCG TCAGCAGGTG 
TTGCCCCTTG ATTCTATTTA TAAGAAGACT CTTCCAGATT GGAAAAGACC TCTGCCTCAT 
TACCGAAATG GAAAATTGTA TTTTAAACCC ATTGGAGATC CCATCTTTGC CCGAGATTTG 
TTAACATTTC CAGATAACGT AGAACATTGT GAAACAGTAT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAATTT GGATGCTGCC AATCATTATA GAAAAGAGGT TGTTAAAATT 
ACACACTGTC CTACACTGCT GACCAGAGAT GGAGATCGCA TTCGAAGTAA TGGAAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCTCCAATG GATAAACTTC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AACAGTGCCT GATCTTAGGG GAACAAATAG ATCTTCTTCA GCAGTACCGC 
ACTGCTGTGT GCAGGTTGGG CAGTGTGAAC AAGGACCTTA ACGGGCAGTT GGAATACCTT 
CACACTCCAG ATATGAAGAA GAAAAAACAG GAACTTGATG AACAAGAGAA GAATCTCAAA 
CTGATAGAGC AAAAACTAGG TATGACTCCC ACACGTAAGT GTAATGACTC ACTGCGTCAT 
CCAATGACAG ACTGTCCAGT TCCTCCTAAG AGAATGAGAA GAGAAGCTAC AAGACAAAAT 
AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126744.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126806.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

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CloneID	OPh124540
Clone ID Related Accession (Same CDS sequence)	XM_007126744.1 , XM_007126744.2
Accession Version	XM_007126744.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006724189.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT TACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGACCAC TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAGG CTGTTTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAGGATG TTCATGAACT TGTACTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCGA TATATAGCGG ATATATTAGA 
AACAGAAAGC CGTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCTCAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCTTCTTT GGACAAGACA GTTAGCGCAT 
ATTTATCATT ACTATATTCA TGGCCCAAAA GGGAATGAAA AACGTACATC AAAAGAAGTT 
GGACCTTTCA ACAACATTGA TATTGAAATT TCTATGTTTG AAAAGGGGAA GACACCTAAG 
ATTATCAACC TAAGGGAGAT ACAAGATGAC ATGCAGACGT TGTATATAAA TACAGCAGCC 
GATAGTTTTG AGTTCAAATC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGA 
TATCACCCTT TTTTATATGA TAGAGAAACG TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA TGATGACTGT TTCATACTTG AGAAAGCAGC AAGAGGGAAA 
AGACCTATTT TTGAATGTTT TTGGAATGGA CGACTAATAC CATATACATC TGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CGATTGAGTG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ACTGACCTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACTCTTT TTACAAGGAT TTTAAATGGA 
CAGGAGCAGC GAATGAAAAT TGACAGAGAA TTTGCTTTAT GGCTGAAGGA CTGTCATGAA 
AAGTATGACA AACAAATAAA ATTTACACTT TTTAAAGGAA TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAGGGCCC CTGGGCGACT TATTCAGCAA TAGAATGGGA TGGAAAAATA 
TACAAAGCAG GACAGCTCGT CAAGACAATC AAGACACTTC CTCTCTTTTA TGGAAGCATT 
GTAAAATTTT TTCTTTATGG TGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA AGCACTGTAT GATGAAATAA AAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTG AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGACG AATTATTGCC AAATGAGATT 
AGGCCTGCTG GAACCCCCAT TGGTGCGTTA AGAATTGAAA TATTGAATAA AAAGGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGTCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATAC TACACTCTCC AAGTGGAAAT AAAGAAATCA TTTCACATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAAAAGTT GGGGAATTAT 
ACATTGAAAT TACAAGTTGT ACTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA ATTTTCTGTC AAAGAGGGTA AACCAGAGAA ATTTTCATTT 
GGTCTTTTGG ATCCTCCTTT TCGTGTTGGA GTCCCATTCA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACTAG TCAACTATCA ACTGATATTC AGCCAGTTTT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AATGGACCAA ATTGTGTCAT TCGAGGTGTT 
ACAGCTAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT TTAATCTGAA GGTTATTCTT 
CCGGGTTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTGCCAGG TCCCCCTCAT 
CGATTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTAG AAGTTTTGGA TGAATCAGAC AATATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTACG TTGTGGATTG CAGTAGTTCT 
GGAACCAGTA TTTTGTCAGG ACCAGCAATT CAAGTTCAGA ATATCAAAAA AGACCAGATG 
CTTAAAGCAA AAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA TTACAGATAT TCAGTGTAGA AGGACAAAAG 
GCAATCCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CAGGAGATAT TATGCATAAC 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCC ATATAACATC AGCTTTAGCA 
GAGAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AGCACTTACT ACAGGGTCTG 
CTTCCTGATG TACAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCA TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATCTAA AATGTGAATT AAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAATAG TTATAATAGT TACAGATCAG TATGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTCTAT CTGCCTTGGG AATTGCTGGG ATTGGACTTG ATAACTCACA TTTGAAAACT 
ACTTTTCAGG AAAATACACA AAGTATAAGT GTGAGAGGCA TCAAATTTAT TCCAGGTCCC 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAG 
CTAATTTCTG GGCCACCAGC TAAACTTCTC CTTGTAGACT GGCCAGAGTT AAAGGAGTCC 
ATTCCAGTGA TTAACGGAAG AGAATTACAG AATCCTCTCA TTGTTCAACT TTGTGATCAA 
TGGGATAATC CAGCACTTGT GCCACAGGTT AAAATAAGCC TTATAAAAGC TAACAATTTA 
AAGCTCACTC CTTCAAACCA ACAGCATAAA ACCGATGAAA GGGGCAGGGC TAATTTGGGA 
GTGTTCAGTG TTTATGCCCC TAGAGGAGAA CATATTATGC AGGTTAAAGC CATTTATAAC 
AAGAATACCA TAGAAGGACC CATAATTAAG TTAATGATTC TTCCAGACCC TGAAAAACCC 
ATTCGTCTCA ATGTTAAATA TGACAAAGAT GCTTCCTTTT TAGCAGGGGG TATTTTCACT 
GATTTTATGA TTACTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAATAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAACTGTCA AACAGTGGGA ACCGACCACC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAGGAT AATGACAAGG AAGATGGCTG CTTCTACTTC 
AGGGATAAAG CAATTCCTAA CAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAGCAA ATATCCTTAA CAGTGAGCAG ATTATAGTGG ATGTTCTACC TAATCAACCT 
GTGAAGTTAG TTCCTGAAAT TCAGCCAGCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAA AGATTTATAT CTTCGTATCA CGGATGACTA CAACAATCAT 
ACTGGAATTG ATTTGGTTGG CACTGTAATG GCTACCATCA AAGGTTCTAA TGAGGAAGAT 
GACACCCCAC TCTTTATTGG GAAAGTTAGA ATACTTGAAT TTCCCTTTGT GAAAGGTTCA 
GCTGAAATCA CGAGTCTAGT TCTGGCAGAA AATAGTCCTG GAAAGGATAG CACTGAGTAT 
TTTATTGTGT TTGAGCCTCG GCTACCAGGC TTATCAAGAA CATTAGAATC ATATATCCTA 
CCATTTATGT TTTACAATGA TGTTAAGAAG CAGCAACAAA TGGCAGCACT TACAAAAGAA 
AAGGACCAAT TATCTAAGTC TATTATAATG TATAGAAGTT TATTTGATGC CAGCAAACAG 
CTTCTTGATG AGATGAAGTG CCAAGTTGAA GAAGCAAAAT TAAAAGAGGC CCAGTTGCGA 
AATGAACTAA AAACACACAA TATTGACATT CCTACAACAC AACAGATGCC ACACATTGAA 
GCACTTTTGA AGAGAAAGAT ATCAGAACAG GAAGAACTAA AGAAAAAACC CAGAAGATCA 
TGTACTCTTC CAAACTATAC TAAAGGCAGT GGAGATGTTT TGGGAAAGAT TGCACACCTG 
GCACAAATTG AAGATGATAG AGCCGCAATG GTTATTTCTT GGCACCTGGC AAGTGACATG 
GACTGTGTGG TCACCCTGAC CACTGATGCT GCACGTCGTA TCTATGACGA AACCCAAGGT 
CGTCAGCAGG TGTTGCCCCT TGATTCTATT TATAAGAAGA CTCTTCCAGA TTGGAAAAGA 
CCTCTGCCTC ATTACCGAAA TGGAAAATTG TATTTTAAAC CCATTGGAGA TCCCATCTTT 
GCCCGAGATT TGTTAACATT TCCAGATAAC GTAGAACATT GTGAAACAGT ATTTGGTATG 
CTGTTAGGAG ACACCATTAT TTTGGATAAT TTGGATGCTG CCAATCATTA TAGAAAAGAG 
GTTGTTAAAA TTACACACTG TCCTACACTG CTGACCAGAG ATGGAGATCG CATTCGAAGT 
AATGGAAAGT TTGGGGGCCT TCAGAATAAA GCCCCTCCAA TGGATAAACT TCGGGGAATG 
GTCTTTGGAG CTCCAGTACC AAAACAGTGC CTGATCTTAG GGGAACAAAT AGATCTTCTT 
CAGCAGTACC GCACTGCTGT GTGCAGGTTG GGCAGTGTGA ACAAGGACCT TAACGGGCAG 
TTGGAATACC TTCACACTCC AGATATGAAG AAGAAAAAAC AGGAACTTGA TGAACAAGAG 
AAGAATCTCA AACTGATAGA GCAAAAACTA GGTATGACTC CCACACGTAA GTGTAATGAC 
TCACTGCGTC ATCCAATGAC AGACTGTCCA GTTCCTCCTA AGAGAATGAG AAGAGAAGCT 
ACAAGACAAA ATAGGATTAT AACCAAAACA GATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126806.1
CDS	223..5661
Translation	MTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126744.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126744.1

ORF Insert Sequence:

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT TACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGACCAC TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAGG CTGTTTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAGGATG TTCATGAACT TGTACTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCGA TATATAGCGG ATATATTAGA 
AACAGAAAGC CGTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCTCAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCTTCTTT GGACAAGACA GTTAGCGCAT 
ATTTATCATT ACTATATTCA TGGCCCAAAA GGGAATGAAA AACGTACATC AAAAGAAGTT 
GGACCTTTCA ACAACATTGA TATTGAAATT TCTATGTTTG AAAAGGGGAA GACACCTAAG 
ATTATCAACC TAAGGGAGAT ACAAGATGAC ATGCAGACGT TGTATATAAA TACAGCAGCC 
GATAGTTTTG AGTTCAAATC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGA 
TATCACCCTT TTTTATATGA TAGAGAAACG TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA TGATGACTGT TTCATACTTG AGAAAGCAGC AAGAGGGAAA 
AGACCTATTT TTGAATGTTT TTGGAATGGA CGACTAATAC CATATACATC TGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CGATTGAGTG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ACTGACCTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACTCTTT TTACAAGGAT TTTAAATGGA 
CAGGAGCAGC GAATGAAAAT TGACAGAGAA TTTGCTTTAT GGCTGAAGGA CTGTCATGAA 
AAGTATGACA AACAAATAAA ATTTACACTT TTTAAAGGAA TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAGGGCCC CTGGGCGACT TATTCAGCAA TAGAATGGGA TGGAAAAATA 
TACAAAGCAG GACAGCTCGT CAAGACAATC AAGACACTTC CTCTCTTTTA TGGAAGCATT 
GTAAAATTTT TTCTTTATGG TGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA AGCACTGTAT GATGAAATAA AAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTG AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGACG AATTATTGCC AAATGAGATT 
AGGCCTGCTG GAACCCCCAT TGGTGCGTTA AGAATTGAAA TATTGAATAA AAAGGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGTCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATAC TACACTCTCC AAGTGGAAAT AAAGAAATCA TTTCACATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAAAAGTT GGGGAATTAT 
ACATTGAAAT TACAAGTTGT ACTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA ATTTTCTGTC AAAGAGGGTA AACCAGAGAA ATTTTCATTT 
GGTCTTTTGG ATCCTCCTTT TCGTGTTGGA GTCCCATTCA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACTAG TCAACTATCA ACTGATATTC AGCCAGTTTT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AATGGACCAA ATTGTGTCAT TCGAGGTGTT 
ACAGCTAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT TTAATCTGAA GGTTATTCTT 
CCGGGTTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTGCCAGG TCCCCCTCAT 
CGATTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTAG AAGTTTTGGA TGAATCAGAC AATATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTACG TTGTGGATTG CAGTAGTTCT 
GGAACCAGTA TTTTGTCAGG ACCAGCAATT CAAGTTCAGA ATATCAAAAA AGACCAGATG 
CTTAAAGCAA AAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA TTACAGATAT TCAGTGTAGA AGGACAAAAG 
GCAATCCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CAGGAGATAT TATGCATAAC 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCC ATATAACATC AGCTTTAGCA 
GAGAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AGCACTTACT ACAGGGTCTG 
CTTCCTGATG TACAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCA TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATCTAA AATGTGAATT AAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAATAG TTATAATAGT TACAGATCAG TATGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTCTAT CTGCCTTGGG AATTGCTGGG ATTGGACTTG ATAACTCACA TTTGAAAACT 
ACTTTTCAGG AAAATACACA AAGTATAAGT GTGAGAGGCA TCAAATTTAT TCCAGGTCCC 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAG 
CTAATTTCTG GGCCACCAGC TAAACTTCTC CTTGTAGACT GGCCAGAGTT AAAGGAGTCC 
ATTCCAGTGA TTAACGGAAG AGAATTACAG AATCCTCTCA TTGTTCAACT TTGTGATCAA 
TGGGATAATC CAGCACTTGT GCCACAGGTT AAAATAAGCC TTATAAAAGC TAACAATTTA 
AAGCTCACTC CTTCAAACCA ACAGCATAAA ACCGATGAAA GGGGCAGGGC TAATTTGGGA 
GTGTTCAGTG TTTATGCCCC TAGAGGAGAA CATATTATGC AGGTTAAAGC CATTTATAAC 
AAGAATACCA TAGAAGGACC CATAATTAAG TTAATGATTC TTCCAGACCC TGAAAAACCC 
ATTCGTCTCA ATGTTAAATA TGACAAAGAT GCTTCCTTTT TAGCAGGGGG TATTTTCACT 
GATTTTATGA TTACTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAATAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAACTGTCA AACAGTGGGA ACCGACCACC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAGGAT AATGACAAGG AAGATGGCTG CTTCTACTTC 
AGGGATAAAG CAATTCCTAA CAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAGCAA ATATCCTTAA CAGTGAGCAG ATTATAGTGG ATGTTCTACC TAATCAACCT 
GTGAAGTTAG TTCCTGAAAT TCAGCCAGCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAA AGATTTATAT CTTCGTATCA CGGATGACTA CAACAATCAT 
ACTGGAATTG ATTTGGTTGG CACTGTAATG GCTACCATCA AAGGTTCTAA TGAGGAAGAT 
GACACCCCAC TCTTTATTGG GAAAGTTAGA ATACTTGAAT TTCCCTTTGT GAAAGGTTCA 
GCTGAAATCA CGAGTCTAGT TCTGGCAGAA AATAGTCCTG GAAAGGATAG CACTGAGTAT 
TTTATTGTGT TTGAGCCTCG GCTACCAGGC TTATCAAGAA CATTAGAATC ATATATCCTA 
CCATTTATGT TTTACAATGA TGTTAAGAAG CAGCAACAAA TGGCAGCACT TACAAAAGAA 
AAGGACCAAT TATCTAAGTC TATTATAATG TATAGAAGTT TATTTGATGC CAGCAAACAG 
CTTCTTGATG AGATGAAGTG CCAAGTTGAA GAAGCAAAAT TAAAAGAGGC CCAGTTGCGA 
AATGAACTAA AAACACACAA TATTGACATT CCTACAACAC AACAGATGCC ACACATTGAA 
GCACTTTTGA AGAGAAAGAT ATCAGAACAG GAAGAACTAA AGAAAAAACC CAGAAGATCA 
TGTACTCTTC CAAACTATAC TAAAGGCAGT GGAGATGTTT TGGGAAAGAT TGCACACCTG 
GCACAAATTG AAGATGATAG AGCCGCAATG GTTATTTCTT GGCACCTGGC AAGTGACATG 
GACTGTGTGG TCACCCTGAC CACTGATGCT GCACGTCGTA TCTATGACGA AACCCAAGGT 
CGTCAGCAGG TGTTGCCCCT TGATTCTATT TATAAGAAGA CTCTTCCAGA TTGGAAAAGA 
CCTCTGCCTC ATTACCGAAA TGGAAAATTG TATTTTAAAC CCATTGGAGA TCCCATCTTT 
GCCCGAGATT TGTTAACATT TCCAGATAAC GTAGAACATT GTGAAACAGT ATTTGGTATG 
CTGTTAGGAG ACACCATTAT TTTGGATAAT TTGGATGCTG CCAATCATTA TAGAAAAGAG 
GTTGTTAAAA TTACACACTG TCCTACACTG CTGACCAGAG ATGGAGATCG CATTCGAAGT 
AATGGAAAGT TTGGGGGCCT TCAGAATAAA GCCCCTCCAA TGGATAAACT TCGGGGAATG 
GTCTTTGGAG CTCCAGTACC AAAACAGTGC CTGATCTTAG GGGAACAAAT AGATCTTCTT 
CAGCAGTACC GCACTGCTGT GTGCAGGTTG GGCAGTGTGA ACAAGGACCT TAACGGGCAG 
TTGGAATACC TTCACACTCC AGATATGAAG AAGAAAAAAC AGGAACTTGA TGAACAAGAG 
AAGAATCTCA AACTGATAGA GCAAAAACTA GGTATGACTC CCACACGTAA GTGTAATGAC 
TCACTGCGTC ATCCAATGAC AGACTGTCCA GTTCCTCCTA AGAGAATGAG AAGAGAAGCT 
ACAAGACAAA ATAGGATTAT AACCAAAACA GATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_007126744.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007126806.1 Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

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CloneID	OPh124540
Clone ID Related Accession (Same CDS sequence)	XM_007126744.1 , XM_007126744.2
Accession Version	XM_007126744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873555.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007126744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT TACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGACCAC TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAGG CTGTTTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAGGATG TTCATGAACT TGTACTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCGA TATATAGCGG ATATATTAGA 
AACAGAAAGC CGTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCTCAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCTTCTTT GGACAAGACA GTTAGCGCAT 
ATTTATCATT ACTATATTCA TGGCCCAAAA GGGAATGAAA AACGTACATC AAAAGAAGTT 
GGACCTTTCA ACAACATTGA TATTGAAATT TCTATGTTTG AAAAGGGGAA GACACCTAAG 
ATTATCAACC TAAGGGAGAT ACAAGATGAC ATGCAGACGT TGTATATAAA TACAGCAGCC 
GATAGTTTTG AGTTCAAATC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGA 
TATCACCCTT TTTTATATGA TAGAGAAACG TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA TGATGACTGT TTCATACTTG AGAAAGCAGC AAGAGGGAAA 
AGACCTATTT TTGAATGTTT TTGGAATGGA CGACTAATAC CATATACATC TGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CGATTGAGTG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ACTGACCTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACTCTTT TTACAAGGAT TTTAAATGGA 
CAGGAGCAGC GAATGAAAAT TGACAGAGAA TTTGCTTTAT GGCTGAAGGA CTGTCATGAA 
AAGTATGACA AACAAATAAA ATTTACACTT TTTAAAGGAA TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAGGGCCC CTGGGCGACT TATTCAGCAA TAGAATGGGA TGGAAAAATA 
TACAAAGCAG GACAGCTCGT CAAGACAATC AAGACACTTC CTCTCTTTTA TGGAAGCATT 
GTAAAATTTT TTCTTTATGG TGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA AGCACTGTAT GATGAAATAA AAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTG AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGACG AATTATTGCC AAATGAGATT 
AGGCCTGCTG GAACCCCCAT TGGTGCGTTA AGAATTGAAA TATTGAATAA AAAGGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGTCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATAC TACACTCTCC AAGTGGAAAT AAAGAAATCA TTTCACATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAAAAGTT GGGGAATTAT 
ACATTGAAAT TACAAGTTGT ACTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA ATTTTCTGTC AAAGAGGGTA AACCAGAGAA ATTTTCATTT 
GGTCTTTTGG ATCCTCCTTT TCGTGTTGGA GTCCCATTCA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACTAG TCAACTATCA ACTGATATTC AGCCAGTTTT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AATGGACCAA ATTGTGTCAT TCGAGGTGTT 
ACAGCTAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT TTAATCTGAA GGTTATTCTT 
CCGGGTTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTGCCAGG TCCCCCTCAT 
CGATTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTAG AAGTTTTGGA TGAATCAGAC AATATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTACG TTGTGGATTG CAGTAGTTCT 
GGAACCAGTA TTTTGTCAGG ACCAGCAATT CAAGTTCAGA ATATCAAAAA AGACCAGATG 
CTTAAAGCAA AAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA TTACAGATAT TCAGTGTAGA AGGACAAAAG 
GCAATCCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CAGGAGATAT TATGCATAAC 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCC ATATAACATC AGCTTTAGCA 
GAGAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AGCACTTACT ACAGGGTCTG 
CTTCCTGATG TACAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCA TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATCTAA AATGTGAATT AAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAATAG TTATAATAGT TACAGATCAG TATGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTCTAT CTGCCTTGGG AATTGCTGGG ATTGGACTTG ATAACTCACA TTTGAAAACT 
ACTTTTCAGG AAAATACACA AAGTATAAGT GTGAGAGGCA TCAAATTTAT TCCAGGTCCC 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAG 
CTAATTTCTG GGCCACCAGC TAAACTTCTC CTTGTAGACT GGCCAGAGTT AAAGGAGTCC 
ATTCCAGTGA TTAACGGAAG AGAATTACAG AATCCTCTCA TTGTTCAACT TTGTGATCAA 
TGGGATAATC CAGCACTTGT GCCACAGGTT AAAATAAGCC TTATAAAAGC TAACAATTTA 
AAGCTCACTC CTTCAAACCA ACAGCATAAA ACCGATGAAA GGGGCAGGGC TAATTTGGGA 
GTGTTCAGTG TTTATGCCCC TAGAGGAGAA CATATTATGC AGGTTAAAGC CATTTATAAC 
AAGAATACCA TAGAAGGACC CATAATTAAG TTAATGATTC TTCCAGACCC TGAAAAACCC 
ATTCGTCTCA ATGTTAAATA TGACAAAGAT GCTTCCTTTT TAGCAGGGGG TATTTTCACT 
GATTTTATGA TTACTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAATAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAACTGTCA AACAGTGGGA ACCGACCACC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAGGAT AATGACAAGG AAGATGGCTG CTTCTACTTC 
AGGGATAAAG CAATTCCTAA CAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAGCAA ATATCCTTAA CAGTGAGCAG ATTATAGTGG ATGTTCTACC TAATCAACCT 
GTGAAGTTAG TTCCTGAAAT TCAGCCAGCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAA AGATTTATAT CTTCGTATCA CGGATGACTA CAACAATCAT 
ACTGGAATTG ATTTGGTTGG CACTGTAATG GCTACCATCA AAGGTTCTAA TGAGGAAGAT 
GACACCCCAC TCTTTATTGG GAAAGTTAGA ATACTTGAAT TTCCCTTTGT GAAAGGTTCA 
GCTGAAATCA CGAGTCTAGT TCTGGCAGAA AATAGTCCTG GAAAGGATAG CACTGAGTAT 
TTTATTGTGT TTGAGCCTCG GCTACCAGGC TTATCAAGAA CATTAGAATC ATATATCCTA 
CCATTTATGT TTTACAATGA TGTTAAGAAG CAGCAACAAA TGGCAGCACT TACAAAAGAA 
AAGGACCAAT TATCTAAGTC TATTATAATG TATAGAAGTT TATTTGATGC CAGCAAACAG 
CTTCTTGATG AGATGAAGTG CCAAGTTGAA GAAGCAAAAT TAAAAGAGGC CCAGTTGCGA 
AATGAACTAA AAACACACAA TATTGACATT CCTACAACAC AACAGATGCC ACACATTGAA 
GCACTTTTGA AGAGAAAGAT ATCAGAACAG GAAGAACTAA AGAAAAAACC CAGAAGATCA 
TGTACTCTTC CAAACTATAC TAAAGGCAGT GGAGATGTTT TGGGAAAGAT TGCACACCTG 
GCACAAATTG AAGATGATAG AGCCGCAATG GTTATTTCTT GGCACCTGGC AAGTGACATG 
GACTGTGTGG TCACCCTGAC CACTGATGCT GCACGTCGTA TCTATGACGA AACCCAAGGT 
CGTCAGCAGG TGTTGCCCCT TGATTCTATT TATAAGAAGA CTCTTCCAGA TTGGAAAAGA 
CCTCTGCCTC ATTACCGAAA TGGAAAATTG TATTTTAAAC CCATTGGAGA TCCCATCTTT 
GCCCGAGATT TGTTAACATT TCCAGATAAC GTAGAACATT GTGAAACAGT ATTTGGTATG 
CTGTTAGGAG ACACCATTAT TTTGGATAAT TTGGATGCTG CCAATCATTA TAGAAAAGAG 
GTTGTTAAAA TTACACACTG TCCTACACTG CTGACCAGAG ATGGAGATCG CATTCGAAGT 
AATGGAAAGT TTGGGGGCCT TCAGAATAAA GCCCCTCCAA TGGATAAACT TCGGGGAATG 
GTCTTTGGAG CTCCAGTACC AAAACAGTGC CTGATCTTAG GGGAACAAAT AGATCTTCTT 
CAGCAGTACC GCACTGCTGT GTGCAGGTTG GGCAGTGTGA ACAAGGACCT TAACGGGCAG 
TTGGAATACC TTCACACTCC AGATATGAAG AAGAAAAAAC AGGAACTTGA TGAACAAGAG 
AAGAATCTCA AACTGATAGA GCAAAAACTA GGTATGACTC CCACACGTAA GTGTAATGAC 
TCACTGCGTC ATCCAATGAC AGACTGTCCA GTTCCTCCTA AGAGAATGAG AAGAGAAGCT 
ACAAGACAAA ATAGGATTAT AACCAAAACA GATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007126806.1
CDS	223..5661
Translation	MTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPLPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLISEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKTPKIINLREIQDDMQTLYINTAADSFEFKSHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSPSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLSTDIQPVFEASGLSLHYEEITNGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILSGPAIQVQNIKKDQMLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDNSHLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLVDWPELKESIPVINGRELQNPLIVQLCDQWDNPALVPQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHIMQVKAIYNKNTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWKLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLYLRITDDYNNHTGIDLVGTVMATIKGSNEEDDTPLFIGKVRILEFPFVKGSAEITSLVLAENSPGKDSTEYFIVFEPRLPGLSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKSIIMYRSLFDASKQLLDEMKCQVEEAKLKEAQLRNELKTHNIDIPTTQQMPHIEALLKRKISEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPIFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRTAVCRLGSVNKDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPMTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_007126744.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007126744.2

ORF Insert Sequence:

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT TACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGACCAC TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAGG CTGTTTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAGGATG TTCATGAACT TGTACTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCGA TATATAGCGG ATATATTAGA 
AACAGAAAGC CGTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCTCAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCTTCTTT GGACAAGACA GTTAGCGCAT 
ATTTATCATT ACTATATTCA TGGCCCAAAA GGGAATGAAA AACGTACATC AAAAGAAGTT 
GGACCTTTCA ACAACATTGA TATTGAAATT TCTATGTTTG AAAAGGGGAA GACACCTAAG 
ATTATCAACC TAAGGGAGAT ACAAGATGAC ATGCAGACGT TGTATATAAA TACAGCAGCC 
GATAGTTTTG AGTTCAAATC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGA 
TATCACCCTT TTTTATATGA TAGAGAAACG TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA TGATGACTGT TTCATACTTG AGAAAGCAGC AAGAGGGAAA 
AGACCTATTT TTGAATGTTT TTGGAATGGA CGACTAATAC CATATACATC TGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CGATTGAGTG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ACTGACCTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACTCTTT TTACAAGGAT TTTAAATGGA 
CAGGAGCAGC GAATGAAAAT TGACAGAGAA TTTGCTTTAT GGCTGAAGGA CTGTCATGAA 
AAGTATGACA AACAAATAAA ATTTACACTT TTTAAAGGAA TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAGGGCCC CTGGGCGACT TATTCAGCAA TAGAATGGGA TGGAAAAATA 
TACAAAGCAG GACAGCTCGT CAAGACAATC AAGACACTTC CTCTCTTTTA TGGAAGCATT 
GTAAAATTTT TTCTTTATGG TGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA AGCACTGTAT GATGAAATAA AAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTG AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGACG AATTATTGCC AAATGAGATT 
AGGCCTGCTG GAACCCCCAT TGGTGCGTTA AGAATTGAAA TATTGAATAA AAAGGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGTCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATAC TACACTCTCC AAGTGGAAAT AAAGAAATCA TTTCACATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAAAAGTT GGGGAATTAT 
ACATTGAAAT TACAAGTTGT ACTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA ATTTTCTGTC AAAGAGGGTA AACCAGAGAA ATTTTCATTT 
GGTCTTTTGG ATCCTCCTTT TCGTGTTGGA GTCCCATTCA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACTAG TCAACTATCA ACTGATATTC AGCCAGTTTT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AATGGACCAA ATTGTGTCAT TCGAGGTGTT 
ACAGCTAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT TTAATCTGAA GGTTATTCTT 
CCGGGTTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTGCCAGG TCCCCCTCAT 
CGATTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTAG AAGTTTTGGA TGAATCAGAC AATATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTACG TTGTGGATTG CAGTAGTTCT 
GGAACCAGTA TTTTGTCAGG ACCAGCAATT CAAGTTCAGA ATATCAAAAA AGACCAGATG 
CTTAAAGCAA AAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA TTACAGATAT TCAGTGTAGA AGGACAAAAG 
GCAATCCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CAGGAGATAT TATGCATAAC 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCC ATATAACATC AGCTTTAGCA 
GAGAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AGCACTTACT ACAGGGTCTG 
CTTCCTGATG TACAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCA TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATCTAA AATGTGAATT AAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAATAG TTATAATAGT TACAGATCAG TATGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTCTAT CTGCCTTGGG AATTGCTGGG ATTGGACTTG ATAACTCACA TTTGAAAACT 
ACTTTTCAGG AAAATACACA AAGTATAAGT GTGAGAGGCA TCAAATTTAT TCCAGGTCCC 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAG 
CTAATTTCTG GGCCACCAGC TAAACTTCTC CTTGTAGACT GGCCAGAGTT AAAGGAGTCC 
ATTCCAGTGA TTAACGGAAG AGAATTACAG AATCCTCTCA TTGTTCAACT TTGTGATCAA 
TGGGATAATC CAGCACTTGT GCCACAGGTT AAAATAAGCC TTATAAAAGC TAACAATTTA 
AAGCTCACTC CTTCAAACCA ACAGCATAAA ACCGATGAAA GGGGCAGGGC TAATTTGGGA 
GTGTTCAGTG TTTATGCCCC TAGAGGAGAA CATATTATGC AGGTTAAAGC CATTTATAAC 
AAGAATACCA TAGAAGGACC CATAATTAAG TTAATGATTC TTCCAGACCC TGAAAAACCC 
ATTCGTCTCA ATGTTAAATA TGACAAAGAT GCTTCCTTTT TAGCAGGGGG TATTTTCACT 
GATTTTATGA TTACTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAATAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAACTGTCA AACAGTGGGA ACCGACCACC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAGGAT AATGACAAGG AAGATGGCTG CTTCTACTTC 
AGGGATAAAG CAATTCCTAA CAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAGCAA ATATCCTTAA CAGTGAGCAG ATTATAGTGG ATGTTCTACC TAATCAACCT 
GTGAAGTTAG TTCCTGAAAT TCAGCCAGCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAA AGATTTATAT CTTCGTATCA CGGATGACTA CAACAATCAT 
ACTGGAATTG ATTTGGTTGG CACTGTAATG GCTACCATCA AAGGTTCTAA TGAGGAAGAT 
GACACCCCAC TCTTTATTGG GAAAGTTAGA ATACTTGAAT TTCCCTTTGT GAAAGGTTCA 
GCTGAAATCA CGAGTCTAGT TCTGGCAGAA AATAGTCCTG GAAAGGATAG CACTGAGTAT 
TTTATTGTGT TTGAGCCTCG GCTACCAGGC TTATCAAGAA CATTAGAATC ATATATCCTA 
CCATTTATGT TTTACAATGA TGTTAAGAAG CAGCAACAAA TGGCAGCACT TACAAAAGAA 
AAGGACCAAT TATCTAAGTC TATTATAATG TATAGAAGTT TATTTGATGC CAGCAAACAG 
CTTCTTGATG AGATGAAGTG CCAAGTTGAA GAAGCAAAAT TAAAAGAGGC CCAGTTGCGA 
AATGAACTAA AAACACACAA TATTGACATT CCTACAACAC AACAGATGCC ACACATTGAA 
GCACTTTTGA AGAGAAAGAT ATCAGAACAG GAAGAACTAA AGAAAAAACC CAGAAGATCA 
TGTACTCTTC CAAACTATAC TAAAGGCAGT GGAGATGTTT TGGGAAAGAT TGCACACCTG 
GCACAAATTG AAGATGATAG AGCCGCAATG GTTATTTCTT GGCACCTGGC AAGTGACATG 
GACTGTGTGG TCACCCTGAC CACTGATGCT GCACGTCGTA TCTATGACGA AACCCAAGGT 
CGTCAGCAGG TGTTGCCCCT TGATTCTATT TATAAGAAGA CTCTTCCAGA TTGGAAAAGA 
CCTCTGCCTC ATTACCGAAA TGGAAAATTG TATTTTAAAC CCATTGGAGA TCCCATCTTT 
GCCCGAGATT TGTTAACATT TCCAGATAAC GTAGAACATT GTGAAACAGT ATTTGGTATG 
CTGTTAGGAG ACACCATTAT TTTGGATAAT TTGGATGCTG CCAATCATTA TAGAAAAGAG 
GTTGTTAAAA TTACACACTG TCCTACACTG CTGACCAGAG ATGGAGATCG CATTCGAAGT 
AATGGAAAGT TTGGGGGCCT TCAGAATAAA GCCCCTCCAA TGGATAAACT TCGGGGAATG 
GTCTTTGGAG CTCCAGTACC AAAACAGTGC CTGATCTTAG GGGAACAAAT AGATCTTCTT 
CAGCAGTACC GCACTGCTGT GTGCAGGTTG GGCAGTGTGA ACAAGGACCT TAACGGGCAG 
TTGGAATACC TTCACACTCC AGATATGAAG AAGAAAAAAC AGGAACTTGA TGAACAAGAG 
AAGAATCTCA AACTGATAGA GCAAAAACTA GGTATGACTC CCACACGTAA GTGTAATGAC 
TCACTGCGTC ATCCAATGAC AGACTGTCCA GTTCCTCCTA AGAGAATGAG AAGAGAAGCT 
ACAAGACAAA ATAGGATTAT AACCAAAACA GATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SMCHD1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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