CHD1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD1
Entrez Gene ID	102981775
Full Name	chromodomain helicase DNA binding protein 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007113512.1	XP_007113574.1	chromodomain-helicase-DNA-binding protein 1-like isoform X2
XM_028493102.1	XP_028348903.1	chromodomain-helicase-DNA-binding protein 1 isoform X1
XM_028493101.1	XP_028348902.1	chromodomain-helicase-DNA-binding protein 1 isoform X1
XM_024129743.1	XP_023985511.1	chromodomain-helicase-DNA-binding protein 1
XM_024129700.1	XP_023985468.1	chromodomain-helicase-DNA-binding protein 1
XM_007113511.1	XP_007113573.1	chromodomain-helicase-DNA-binding protein 1-like isoform X1
XM_024129700.2	XP_023985468.1	chromodomain-helicase-DNA-binding protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following CHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh121956	XM_007113511.1 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh121957	XM_007113512.1 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh284722	XM_024129743.1 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh284722	XM_024129700.2 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh331091	XM_028493101.1 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh331091	XM_028493102.1 Latest version!	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OPh284722	XM_024129700.1	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD1 ( XM_007113511.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007113573.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh121956
Clone ID Related Accession (Same CDS sequence)	XM_007113511.1
Accession Version	XM_007113511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714501.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAACGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007113573.1
CDS	142..5271
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_007113511.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007113511.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAACGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_007113512.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007113574.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

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CloneID	OPh121957
Clone ID Related Accession (Same CDS sequence)	XM_007113512.1
Accession Version	XM_007113512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714501.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAACGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCATTGAG TGAATCCAAG TCTGATAGCA AGGAAAGATC TAAGAAATCT 
TCAGTGTCAG ATGCTCCAGT TCATATCACT GCAAGTGGTG AACCAGTTCC CATATCTGAA 
GAATCTGAAG AGCTAGATCA GAAGACATTC AGCATATGTA AAGAAAGAAT GAGGCCTGTC 
AAAGCAGCTT TGAAGCAACT TGATAGGCCC GAGAAAGGCC TTTCAGAAAG AGAACAACTG 
GAACATACTA GACAGTGCTT AATAAAAATT GGAGACCATA TCACGGAATG TCTGAAAGAG 
TATACAAATC CTGAGCAAAT CAAGCAGTGG AGAAAAAACC TGTGGATTTT TGTATCTAAG 
TTTACTGAGT TTGATGCAAG AAAATTACAT AAATTATATA AGCATGCTAT TAAAAAACGG 
CAGGAATCTC AGCAAAATAA TGACCAGAAC AGCAACTTGA ATACTCAAGT GATTAGAAAT 
CCAGATGTGG AAAGATTAAA AGAGAATACA AATCATGATG ATAGCAGCAG GGACAGTTAT 
TCATCTGATA GGCACTTATC TCAATACCAT GATCATCATA AAGACCGACA TCAGGGAGAT 
TCTTATAAAA AGAGTGACTC TAGGAAAAGA CCCTACTCTT CTTTTAGTAA TGGTAAAGAT 
CATCGTGATT GGGATCACTA CAAGCAAGAC AGCAGATACT ATAGTGATAG AGAGAAACAC 
AGAAAACTGG ATGATCACAG GAGTAGAGAC CACAGGTCAA ATTTGGAAGG AAGTTTAAAA 
GACAGAACTC ATTCTGATCA TCGTTCTCAT TCAGATCATC GGTTGCATTC AGACCATCGG 
TCAAGTTCTG AATATACACA CCATAAATCT TCCAGGGATT ATAGGTATCA CTCAGATTGG 
CAATTGGACC ATAGAGCTTC CAGTAGTGGC TCTAGATCAC CACTAGATCA GAGGTCTCCT 
TATGGCTCCA GATCTCCATT TGAACACTCA GTTGAACACA GAAGTACACC TGAGCATACC 
TGGAGTAGTC GGAAAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007113574.1
CDS	142..5142
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGALSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_007113512.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007113512.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAACGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCATTGAG TGAATCCAAG TCTGATAGCA AGGAAAGATC TAAGAAATCT 
TCAGTGTCAG ATGCTCCAGT TCATATCACT GCAAGTGGTG AACCAGTTCC CATATCTGAA 
GAATCTGAAG AGCTAGATCA GAAGACATTC AGCATATGTA AAGAAAGAAT GAGGCCTGTC 
AAAGCAGCTT TGAAGCAACT TGATAGGCCC GAGAAAGGCC TTTCAGAAAG AGAACAACTG 
GAACATACTA GACAGTGCTT AATAAAAATT GGAGACCATA TCACGGAATG TCTGAAAGAG 
TATACAAATC CTGAGCAAAT CAAGCAGTGG AGAAAAAACC TGTGGATTTT TGTATCTAAG 
TTTACTGAGT TTGATGCAAG AAAATTACAT AAATTATATA AGCATGCTAT TAAAAAACGG 
CAGGAATCTC AGCAAAATAA TGACCAGAAC AGCAACTTGA ATACTCAAGT GATTAGAAAT 
CCAGATGTGG AAAGATTAAA AGAGAATACA AATCATGATG ATAGCAGCAG GGACAGTTAT 
TCATCTGATA GGCACTTATC TCAATACCAT GATCATCATA AAGACCGACA TCAGGGAGAT 
TCTTATAAAA AGAGTGACTC TAGGAAAAGA CCCTACTCTT CTTTTAGTAA TGGTAAAGAT 
CATCGTGATT GGGATCACTA CAAGCAAGAC AGCAGATACT ATAGTGATAG AGAGAAACAC 
AGAAAACTGG ATGATCACAG GAGTAGAGAC CACAGGTCAA ATTTGGAAGG AAGTTTAAAA 
GACAGAACTC ATTCTGATCA TCGTTCTCAT TCAGATCATC GGTTGCATTC AGACCATCGG 
TCAAGTTCTG AATATACACA CCATAAATCT TCCAGGGATT ATAGGTATCA CTCAGATTGG 
CAATTGGACC ATAGAGCTTC CAGTAGTGGC TCTAGATCAC CACTAGATCA GAGGTCTCCT 
TATGGCTCCA GATCTCCATT TGAACACTCA GTTGAACACA GAAGTACACC TGAGCATACC 
TGGAGTAGTC GGAAAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_024129743.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023985511.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh284722
Clone ID Related Accession (Same CDS sequence)	XM_024129743.1 , XM_024129700.1 , XM_024129700.2
Accession Version	XM_024129743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873548.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023985511.1
CDS	166..5295
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_024129743.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024129743.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_024129700.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023985468.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

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CloneID	OPh284722
Clone ID Related Accession (Same CDS sequence)	XM_024129743.1 , XM_024129700.1 , XM_024129700.2
Accession Version	XM_024129700.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873548.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023985468.1
CDS	557..5686
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_024129700.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024129700.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_024129700.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023985468.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X3, mRNA.

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CloneID	OPh284722
Clone ID Related Accession (Same CDS sequence)	XM_024129743.1 , XM_024129700.1 , XM_024129700.2
Accession Version	XM_024129700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_024129700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023985468.1
CDS	492..5621
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_024129700.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024129700.2

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGTTGAGT GAATCCAAGT CTGATAGCAA GGAAAGATCT 
AAGAAATCTT CAGTGTCAGA TGCTCCAGTT CATATCACTG CAAGTGGTGA ACCAGTTCCC 
ATATCTGAAG AATCTGAAGA GCTAGATCAG AAGACATTCA GCATATGTAA AGAAAGAATG 
AGGCCTGTCA AAGCAGCTTT GAAGCAACTT GATAGGCCCG AGAAAGGCCT TTCAGAAAGA 
GAACAACTGG AACATACTAG ACAGTGCTTA ATAAAAATTG GAGACCATAT CACGGAATGT 
CTGAAAGAGT ATACAAATCC TGAGCAAATC AAGCAGTGGA GAAAAAACCT GTGGATTTTT 
GTATCTAAGT TTACTGAGTT TGATGCAAGA AAATTACATA AATTATATAA GCATGCTATT 
AAAAAACGGC AGGAATCTCA GCAAAATAAT GACCAGAACA GCAACTTGAA TACTCAAGTG 
ATTAGAAATC CAGATGTGGA AAGATTAAAA GAGAATACAA ATCATGATGA TAGCAGCAGG 
GACAGTTATT CATCTGATAG GCACTTATCT CAATACCATG ATCATCATAA AGACCGACAT 
CAGGGAGATT CTTATAAAAA GAGTGACTCT AGGAAAAGAC CCTACTCTTC TTTTAGTAAT 
GGTAAAGATC ATCGTGATTG GGATCACTAC AAGCAAGACA GCAGATACTA TAGTGATAGA 
GAGAAACACA GAAAACTGGA TGATCACAGG AGTAGAGACC ACAGGTCAAA TTTGGAAGGA 
AGTTTAAAAG ACAGAACTCA TTCTGATCAT CGTTCTCATT CAGATCATCG GTTGCATTCA 
GACCATCGGT CAAGTTCTGA ATATACACAC CATAAATCTT CCAGGGATTA TAGGTATCAC 
TCAGATTGGC AATTGGACCA TAGAGCTTCC AGTAGTGGCT CTAGATCACC ACTAGATCAG 
AGGTCTCCTT ATGGCTCCAG ATCTCCATTT GAACACTCAG TTGAACACAG AAGTACACCT 
GAGCATACCT GGAGTAGTCG GAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_028493102.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028348903.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

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CloneID	OPh331091
Clone ID Related Accession (Same CDS sequence)	XM_028493102.1 , XM_028493101.1
Accession Version	XM_028493102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGGATGAG ATCACTTCTG TGAAACATCC AAATAAAAAA 
GTTAAAACAG AAAGAGACAG TGAAGAAAAA CCTGAGCCAG ATGTTTGTAT AAAGAAGGAA 
CCAGAAGAAA AGAGGGAAGC GAAAGAAAAG GAAAATAAAA GAGAACTTAA AAGGGAGATA 
AAAGAAAAAG AGGATAAGAA AGATATAAAG GAAAAAGATT TTAAAGAGAA AAGAGAAAAC 
AAAGTAAAAG AAGCTATACA GAAAGAAAAA GACATAAAGG AAGAAAAGTT GAGTGAATCC 
AAGTCTGATA GCAAGGAAAG ATCTAAGAAA TCTTCAGTGT CAGATGCTCC AGTTCATATC 
ACTGCAAGTG GTGAACCAGT TCCCATATCT GAAGAATCTG AAGAGCTAGA TCAGAAGACA 
TTCAGCATAT GTAAAGAAAG AATGAGGCCT GTCAAAGCAG CTTTGAAGCA ACTTGATAGG 
CCCGAGAAAG GCCTTTCAGA AAGAGAACAA CTGGAACATA CTAGACAGTG CTTAATAAAA 
ATTGGAGACC ATATCACGGA ATGTCTGAAA GAGTATACAA ATCCTGAGCA AATCAAGCAG 
TGGAGAAAAA ACCTGTGGAT TTTTGTATCT AAGTTTACTG AGTTTGATGC AAGAAAATTA 
CATAAATTAT ATAAGCATGC TATTAAAAAA CGGCAGGAAT CTCAGCAAAA TAATGACCAG 
AACAGCAACT TGAATACTCA AGTGATTAGA AATCCAGATG TGGAAAGATT AAAAGAGAAT 
ACAAATCATG ATGATAGCAG CAGGGACAGT TATTCATCTG ATAGGCACTT ATCTCAATAC 
CATGATCATC ATAAAGACCG ACATCAGGGA GATTCTTATA AAAAGAGTGA CTCTAGGAAA 
AGACCCTACT CTTCTTTTAG TAATGGTAAA GATCATCGTG ATTGGGATCA CTACAAGCAA 
GACAGCAGAT ACTATAGTGA TAGAGAGAAA CACAGAAAAC TGGATGATCA CAGGAGTAGA 
GACCACAGGT CAAATTTGGA AGGAAGTTTA AAAGACAGAA CTCATTCTGA TCATCGTTCT 
CATTCAGATC ATCGGTTGCA TTCAGACCAT CGGTCAAGTT CTGAATATAC ACACCATAAA 
TCTTCCAGGG ATTATAGGTA TCACTCAGAT TGGCAATTGG ACCATAGAGC TTCCAGTAGT 
GGCTCTAGAT CACCACTAGA TCAGAGGTCT CCTTATGGCT CCAGATCTCC ATTTGAACAC 
TCAGTTGAAC ACAGAAGTAC ACCTGAGCAT ACCTGGAGTA GTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028348903.1
CDS	166..5559
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKDEITSVKHPNKKVKTERDSEEKPEPDVCIKKEPEEKREAKEKENKRELKREIKEKEDKKDIKEKDFKEKRENKVKEAIQKEKDIKEEKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_028493102.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028493102.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGGATGAG ATCACTTCTG TGAAACATCC AAATAAAAAA 
GTTAAAACAG AAAGAGACAG TGAAGAAAAA CCTGAGCCAG ATGTTTGTAT AAAGAAGGAA 
CCAGAAGAAA AGAGGGAAGC GAAAGAAAAG GAAAATAAAA GAGAACTTAA AAGGGAGATA 
AAAGAAAAAG AGGATAAGAA AGATATAAAG GAAAAAGATT TTAAAGAGAA AAGAGAAAAC 
AAAGTAAAAG AAGCTATACA GAAAGAAAAA GACATAAAGG AAGAAAAGTT GAGTGAATCC 
AAGTCTGATA GCAAGGAAAG ATCTAAGAAA TCTTCAGTGT CAGATGCTCC AGTTCATATC 
ACTGCAAGTG GTGAACCAGT TCCCATATCT GAAGAATCTG AAGAGCTAGA TCAGAAGACA 
TTCAGCATAT GTAAAGAAAG AATGAGGCCT GTCAAAGCAG CTTTGAAGCA ACTTGATAGG 
CCCGAGAAAG GCCTTTCAGA AAGAGAACAA CTGGAACATA CTAGACAGTG CTTAATAAAA 
ATTGGAGACC ATATCACGGA ATGTCTGAAA GAGTATACAA ATCCTGAGCA AATCAAGCAG 
TGGAGAAAAA ACCTGTGGAT TTTTGTATCT AAGTTTACTG AGTTTGATGC AAGAAAATTA 
CATAAATTAT ATAAGCATGC TATTAAAAAA CGGCAGGAAT CTCAGCAAAA TAATGACCAG 
AACAGCAACT TGAATACTCA AGTGATTAGA AATCCAGATG TGGAAAGATT AAAAGAGAAT 
ACAAATCATG ATGATAGCAG CAGGGACAGT TATTCATCTG ATAGGCACTT ATCTCAATAC 
CATGATCATC ATAAAGACCG ACATCAGGGA GATTCTTATA AAAAGAGTGA CTCTAGGAAA 
AGACCCTACT CTTCTTTTAG TAATGGTAAA GATCATCGTG ATTGGGATCA CTACAAGCAA 
GACAGCAGAT ACTATAGTGA TAGAGAGAAA CACAGAAAAC TGGATGATCA CAGGAGTAGA 
GACCACAGGT CAAATTTGGA AGGAAGTTTA AAAGACAGAA CTCATTCTGA TCATCGTTCT 
CATTCAGATC ATCGGTTGCA TTCAGACCAT CGGTCAAGTT CTGAATATAC ACACCATAAA 
TCTTCCAGGG ATTATAGGTA TCACTCAGAT TGGCAATTGG ACCATAGAGC TTCCAGTAGT 
GGCTCTAGAT CACCACTAGA TCAGAGGTCT CCTTATGGCT CCAGATCTCC ATTTGAACAC 
TCAGTTGAAC ACAGAAGTAC ACCTGAGCAT ACCTGGAGTA GTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD1 ( XM_028493101.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028348902.1 Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

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CloneID	OPh331091
Clone ID Related Accession (Same CDS sequence)	XM_028493102.1 , XM_028493101.1
Accession Version	XM_028493101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGGATGAG ATCACTTCTG TGAAACATCC AAATAAAAAA 
GTTAAAACAG AAAGAGACAG TGAAGAAAAA CCTGAGCCAG ATGTTTGTAT AAAGAAGGAA 
CCAGAAGAAA AGAGGGAAGC GAAAGAAAAG GAAAATAAAA GAGAACTTAA AAGGGAGATA 
AAAGAAAAAG AGGATAAGAA AGATATAAAG GAAAAAGATT TTAAAGAGAA AAGAGAAAAC 
AAAGTAAAAG AAGCTATACA GAAAGAAAAA GACATAAAGG AAGAAAAGTT GAGTGAATCC 
AAGTCTGATA GCAAGGAAAG ATCTAAGAAA TCTTCAGTGT CAGATGCTCC AGTTCATATC 
ACTGCAAGTG GTGAACCAGT TCCCATATCT GAAGAATCTG AAGAGCTAGA TCAGAAGACA 
TTCAGCATAT GTAAAGAAAG AATGAGGCCT GTCAAAGCAG CTTTGAAGCA ACTTGATAGG 
CCCGAGAAAG GCCTTTCAGA AAGAGAACAA CTGGAACATA CTAGACAGTG CTTAATAAAA 
ATTGGAGACC ATATCACGGA ATGTCTGAAA GAGTATACAA ATCCTGAGCA AATCAAGCAG 
TGGAGAAAAA ACCTGTGGAT TTTTGTATCT AAGTTTACTG AGTTTGATGC AAGAAAATTA 
CATAAATTAT ATAAGCATGC TATTAAAAAA CGGCAGGAAT CTCAGCAAAA TAATGACCAG 
AACAGCAACT TGAATACTCA AGTGATTAGA AATCCAGATG TGGAAAGATT AAAAGAGAAT 
ACAAATCATG ATGATAGCAG CAGGGACAGT TATTCATCTG ATAGGCACTT ATCTCAATAC 
CATGATCATC ATAAAGACCG ACATCAGGGA GATTCTTATA AAAAGAGTGA CTCTAGGAAA 
AGACCCTACT CTTCTTTTAG TAATGGTAAA GATCATCGTG ATTGGGATCA CTACAAGCAA 
GACAGCAGAT ACTATAGTGA TAGAGAGAAA CACAGAAAAC TGGATGATCA CAGGAGTAGA 
GACCACAGGT CAAATTTGGA AGGAAGTTTA AAAGACAGAA CTCATTCTGA TCATCGTTCT 
CATTCAGATC ATCGGTTGCA TTCAGACCAT CGGTCAAGTT CTGAATATAC ACACCATAAA 
TCTTCCAGGG ATTATAGGTA TCACTCAGAT TGGCAATTGG ACCATAGAGC TTCCAGTAGT 
GGCTCTAGAT CACCACTAGA TCAGAGGTCT CCTTATGGCT CCAGATCTCC ATTTGAACAC 
TCAGTTGAAC ACAGAAGTAC ACCTGAGCAT ACCTGGAGTA GTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028348902.1
CDS	495..5888
Translation	MNGHSDEESVRNSSGESSHSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDNSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLKKQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEERDKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKIIGQKKRQIDSSEEEEEDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSVSERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDNSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYIIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSKDLARKEAQRLSGAGGSKRRKVRAKKNKAMKSIKMKEEIKSDSSPLPSEKSDEDDDKDEITSVKHPNKKVKTERDSEEKPEPDVCIKKEPEEKREAKEKENKRELKREIKEKEDKKDIKEKDFKEKRENKVKEAIQKEKDIKEEKLSESKSDSKERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNNDQNSNLNTQVIRNPDVERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRTHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQLDHRASSSGSRSPLDQRSPYGSRSPFEHSVEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	XM_028493101.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon chromodomain helicase DNA binding protein 1 (CHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028493101.1

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAACAGTA GTGGAGAGTC AAGCCATTCG 
GATGATGATT CTGGGTCAGC GTCAGGCTCT GGATCTGGTT CAAGTTCTGG AAGCAGTAGT 
GATGGAAGCA GTAGCCAGTC AGGTAGCAGT GACTCTGACT CTGGATCCGA ATCGGGCAGT 
CAGTCAGAGT CTGAGTCAGA CAATTCCCGA GAAAATAAAG TTCAAGCAAA ACCACCGAAA 
GTTGATGGAG CTGAGTTTTG GAAATCTAGC CCTAGTATTC TGGCTGTTCA GAGATCTGCA 
ATGCTCAAGA AGCAGCAACA GCAGCAACAT CAAGCTTCAT CTAATAGTGG ATCAGAAGAG 
GATTCTTCTA GCAGTGAAGA TTCCGATGAC TCATCGAGTG AGGTCAAAAG GAAAAAGCAT 
AAAGATGAAG ATTGGCAGAT GTCTGGGTCA GGATCTCCAT CTCAGTCTGG TTCAGATTCA 
GAATCTGAAG AAGAAAGAGA TAAAAGTAGT TGTGATGAAA CAGAATCTGA TTATGAGCCA 
AAAAACAAAG TTAAAAGTAG AAAACCTCAA AATAGATCTA AGTCAAAAAA TGGAAAAAAG 
ATTATTGGAC AAAAAAAGAG ACAGATTGAT TCATCTGAGG AGGAGGAGGA GGATGAAGAA 
GACTATGATA ATGATAAAAG AAGCTCTCGT CGACAAGCAA CTGTCAATGT TAGCTATAAG 
GAAGATGAAG AAATGAAAAC AGATTCTGAT GACCTACTTG AAGTCTGTGG AGAGGATGTT 
CCTCAACCCG AGGAAGAGGA ATTTGAAACA ATAGAACGGT TTATGGATTG TCGGATTGGG 
AGAAAAGGAG CTACTGGTGC TACTACAACC ATCTATGCAG TTGAAGCAGA TGGTGACCCA 
AATGCAGGAT TTGAAAAAAA CAAGGAACCA GGAGAGATCC AGTATTTAAT TAAATGGAAA 
GGATGGTCAC ATATCCACAA TACTTGGGAG ACAGAAGAAA CCCTTAAGCA GCAGAATGTT 
AGAGGAATGA AAAAATTAGA TAATTATAAG AAAAAAGATC AGGAAACAAA AAGATGGTTG 
AAAAATGCTT CTCCAGAAGA TGTGGAATAT TATAATTGCC AGCAAGAACT TACAGATGAT 
CTCCATAAGC AGTATCAAAT AGTGGAGCGA ATAATTGCTC ATTCCAATCA AAAGTCAGCT 
GCTGGCTATC CTGATTATTA TTGCAAATGG CAGGGCCTTC CATACTCAGA GTGCAGCTGG 
GAAGATGGAG CTCTCATTTC CAAAAAGTTT CAGGCGTGCA TAGATGAGTA TTTCAGCAGA 
AATCAATCAA AAACTACTCC TTTTAAAGAC TGCAAAGTTT TAAAACAAAG GCCAAGATTT 
GTAGCCCTAA AGAAGCAGCC ATCCTATATT GGAGGACATG AGGGATTAGA ATTAAGAGAT 
TATCAACTGA ATGGCTTAAA TTGGCTTGCT CACTCTTGGT GCAAAGGAAA TAGTTGCATA 
CTTGCTGATG AAATGGGCCT TGGAAAAACA ATACAGACGA TCTCATTTCT GAACTATTTG 
TTTCATGAAC ACCAATTATA TGGACCTTTT CTATTAGTAG TACCACTCTC CACTCTCACT 
TCCTGGCAAA GGGAGATTCA AACATGGGCT TCTCAAATGA ATGCTGTGGT TTATTTAGGT 
GACATCAACA GCAGAAACAT GATAAGAACT CATGAATGGA TGCATCCCCA GACCAAACGG 
TTAAAATTTA ATATACTGTT AACAACTTAT GAAATCTTAT TGAAGGATAA GGCATTCCTT 
GGAGGTCTAA ATTGGGCATT TATAGGTGTA GACGAAGCAC ATCGATTAAA GAATGATGAT 
TCTCTTCTGT ATAAAACTTT AATAGATTTT AAGTCCAATC ACCGTCTCCT TATCACTGGA 
ACTCCTCTGC AAAACTCCCT CAAAGAGCTC TGGTCTTTGC TACATTTCAT TATGCCAGAA 
AAGTTTTCTT CCTGGGAAGA TTTTGAAGAA GAACATGGCA AAGGCAGAGA ATATGGTTAT 
GCAAGCCTCC ACAAGGAGCT TGAGCCATTT CTGTTACGCA GGGTTAAAAA AGATGTGGAA 
AAATCTCTTC CTGCCAAGGT TGAGCAGATT TTAAGAATGG AAATGAGTGC TTTACAGAAA 
CAATACTACA AATGGATATT AACTAGGAAT TACAAAGCCC TCAGCAAAGG TTCCAAGGGC 
AGTACCTCAG GCTTTTTGAA TATTATGATG GAGCTAAAGA AATGTTGTAA CCATTGCTAC 
CTCATTAAAC CACCAGATAA TAATGAATTC TATAATAAAC AGGAGGCCTT ACAACACTTA 
ATCCGTAGTA GCGGAAAATT AATTCTTCTT GACAAACTGT TAATTCGCCT AAGAGAGCGA 
GGCAATAGAG TTCTTATTTT TTCTCAAATG GTGCGGATGT TAGATATACT TGCAGAATAT 
TTGAAGTATC GTCAATTCCC CTTTCAAAGA TTAGATGGAT CAATAAAAGG GGAGCTGAGG 
AAACAAGCTC TAGATCATTT TAATGCTGAA GGATCAGAGG ATTTCTGCTT TTTGCTTTCC 
ACAAGAGCTG GAGGTTTAGG AATTAACTTA GCCTCAGCTG ACACTGTTGT TATATTTGAT 
TCTGATTGGA ATCCACAAAA TGATCTTCAG GCACAGGCTA GAGCTCATCG AATTGGGCAA 
AAGAAACAGG TGAACATTTA TCGTCTAGTT ACAAAGGGAT CAGTTGAAGA GGATATTCTT 
GAAAGGGCCA AAAAGAAGAT GGTTTTAGAT CATCTTGTGA TTCAAAGAAT GGACACAACT 
GGGAAGACAG TATTACACAC AGGTTCTGCT CCCTCAAGTT CTACTCCTTT CAATAAAGAA 
GAGTTATCAG CCATTTTAAA GTTTGGTGCT GAGGAACTTT TTAAGGAGCC CGAGGGAGAA 
GAACAAGAGC CCCAGGAAAT GGATATAGAT GAAATCTTGA AGAGGGCTGA AACTCATGAA 
AATGAACCAG GACCTTTAAC TGTAGGAGAT GAATTACTTT CTCAGTTCAA GGTTGCCAAC 
TTTTCAAATA TGGATGAGGA TGACATTGAG TTGGAACCTG AAAGAAATTC AAAGAATTGG 
GAAGAAATCA TTCCTGAAGA TCAACGAAGA CGATTAGAAG AGGAAGAAAG ACAAAAGGAA 
CTTGAAGAAA TTTATATGCT CCCCAGAATG AGAAACTGTG CAAAACAGAT TAGTTTCAAT 
GGAAGTGAAG GGAGGCGCAG TAGAAGTAGG AGATACTCTG GATCTGATAG TGATTCAGTC 
TCAGAAAGGA AAAGGCCAAA GAAACGTGGA AGACCACGAA CTATCCCTCG GGAAAATATT 
AAAGGATTTA GTGATGCAGA AATTCGGCGG TTTATCAAGA GTTACAAGAA ATTTGGTGGC 
CCTCTGGAAA GATTAGATGC AATTGCTCGA GATGCTGAAT TAGTTGATAA GTCAGAGACA 
GACCTCAGAC GACTAGGAGA ATTGGTACAT AATGGTTGCA TTAAAGCTTT AAAGGACAAT 
TCTTCAGGAA CAGAACGAAC AGGTGGTAGA CTTGGAAAAG TAAAGGGTCC GACATTCCGA 
ATATCAGGAG TACAGGTGAA TGCCAAGCTA GTCATCTCCC ATGAAGAAGA ATTAATACCG 
TTGCACAAAT CCATTCCTTC AGATCCAGAA GAAAGAAAGC AGTATATCAT CCCATGCCAC 
ACGAAGGCAG CTCATTTTGA TATAGACTGG GGCAAAGAAG ATGATTCCAA TTTGTTAATT 
GGTATCTACG AATACGGATA TGGAAGCTGG GAAATGATTA AAATGGATCC TGACCTCAGT 
CTGACACACA AGATTCTTCC AGATGATCCT GATAAAAAAC CACAAGCAAA ACAGTTACAG 
ACACGTGCGG ACTACCTCAT CAAATTACTT AGTAAAGATC TTGCAAGAAA AGAAGCTCAG 
AGGCTTTCTG GTGCAGGAGG TTCAAAAAGG AGGAAAGTAA GAGCTAAGAA GAATAAAGCA 
ATGAAGTCTA TAAAAATGAA AGAGGAAATA AAGAGCGATT CTTCTCCCCT GCCCTCAGAA 
AAGTCTGATG AAGATGATGA TAAGGATGAG ATCACTTCTG TGAAACATCC AAATAAAAAA 
GTTAAAACAG AAAGAGACAG TGAAGAAAAA CCTGAGCCAG ATGTTTGTAT AAAGAAGGAA 
CCAGAAGAAA AGAGGGAAGC GAAAGAAAAG GAAAATAAAA GAGAACTTAA AAGGGAGATA 
AAAGAAAAAG AGGATAAGAA AGATATAAAG GAAAAAGATT TTAAAGAGAA AAGAGAAAAC 
AAAGTAAAAG AAGCTATACA GAAAGAAAAA GACATAAAGG AAGAAAAGTT GAGTGAATCC 
AAGTCTGATA GCAAGGAAAG ATCTAAGAAA TCTTCAGTGT CAGATGCTCC AGTTCATATC 
ACTGCAAGTG GTGAACCAGT TCCCATATCT GAAGAATCTG AAGAGCTAGA TCAGAAGACA 
TTCAGCATAT GTAAAGAAAG AATGAGGCCT GTCAAAGCAG CTTTGAAGCA ACTTGATAGG 
CCCGAGAAAG GCCTTTCAGA AAGAGAACAA CTGGAACATA CTAGACAGTG CTTAATAAAA 
ATTGGAGACC ATATCACGGA ATGTCTGAAA GAGTATACAA ATCCTGAGCA AATCAAGCAG 
TGGAGAAAAA ACCTGTGGAT TTTTGTATCT AAGTTTACTG AGTTTGATGC AAGAAAATTA 
CATAAATTAT ATAAGCATGC TATTAAAAAA CGGCAGGAAT CTCAGCAAAA TAATGACCAG 
AACAGCAACT TGAATACTCA AGTGATTAGA AATCCAGATG TGGAAAGATT AAAAGAGAAT 
ACAAATCATG ATGATAGCAG CAGGGACAGT TATTCATCTG ATAGGCACTT ATCTCAATAC 
CATGATCATC ATAAAGACCG ACATCAGGGA GATTCTTATA AAAAGAGTGA CTCTAGGAAA 
AGACCCTACT CTTCTTTTAG TAATGGTAAA GATCATCGTG ATTGGGATCA CTACAAGCAA 
GACAGCAGAT ACTATAGTGA TAGAGAGAAA CACAGAAAAC TGGATGATCA CAGGAGTAGA 
GACCACAGGT CAAATTTGGA AGGAAGTTTA AAAGACAGAA CTCATTCTGA TCATCGTTCT 
CATTCAGATC ATCGGTTGCA TTCAGACCAT CGGTCAAGTT CTGAATATAC ACACCATAAA 
TCTTCCAGGG ATTATAGGTA TCACTCAGAT TGGCAATTGG ACCATAGAGC TTCCAGTAGT 
GGCTCTAGAT CACCACTAGA TCAGAGGTCT CCTTATGGCT CCAGATCTCC ATTTGAACAC 
TCAGTTGAAC ACAGAAGTAC ACCTGAGCAT ACCTGGAGTA GTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CHD1 cDNA ORF clone, Physeter catodon(sperm whale)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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