HECW1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	102979386
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007124963.1	XP_007125025.1	E3 ubiquitin-protein ligase HECW1
XM_007124963.2	XP_007125025.1	E3 ubiquitin-protein ligase HECW1

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh122976	XM_007124963.1 Latest version!	Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPh304051	XM_007124963.2 Latest version!	Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_007124963.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007125025.1 Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Price & Availability↑

CloneID	OPh122976
Clone ID Related Accession (Same CDS sequence)	XM_007124963.1
Accession Version	XM_007124963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006724094.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CATCCAGAAA CTCCCAGAAC CGACGCCGGT GCAAGGAGCC CCTCCGCCAC 
AGCTACAACC CGGGCCAGTT CCACAGCATG GCCATTAGGA CCGGCCCCCA TGGCGCTGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACTCTC 
ATGGTCAGCA GCTCCTGCTA CAGCATAGGG CACTCGCAGG ACCTGGTGAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATCGA TGAGGTCTTG 
TCGGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCACTG GTTCTCATCG GGGCCAGATC 
CTCTGGAAGA TCGATGCCAG CTCTTACTTT GTGGACCCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCCTTGCGA GCCACCACCC CCAGTGTCAC CGTCAAAAAC 
TCAGCAGCTC CTATTTTTAA AAGTATTGGC TCTGATGAGA CTGTACAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGGAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCACGG ACAGGAGAGG CGATCCAGGA TTGCCGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAGAGACT CCTGGAAAGA CATGCCATAG GGGACAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGACGATGAG GAGATTTCCC TGAGTACCGA ACCTGAGTCT 
GCAGAAATTC AGGACAGCCC CATGAATAGC CTGGTGGCAA GCAGCCGTGG GGAGCCCCCT 
TGCACTAATG CACCAGAGCC CTCGGGGACC TCAAGGCCGG AGCAGCTGAG CCAGGGCAGC 
GCGGTGGGCC CTGCAGGGAA CGAGAGCCTG GAGCTTGCCA GTCCGGTTGA GGCCGCCGCA 
GGTACCACGG AGGCAGGAGA TGATGGCACG GTCTGTGTGA GGCCTGAGGG GGCTGGGGAG 
CTTCTGGCCC CCTGGCCAGA GGACACGGAG CCCGCCCTGA GCGCGGAAGA ACTGGCCGAG 
CGGCTGGACC TGGGCGGGGA GGCGGCCCTA CCCGGGCTGC TGGAGGACGG TCGAGCCCCG 
GGCAGCCCCG GGCAGCCAGT GGCCAAGGAG GTGGCGACAG AGAGCCGGGC TGGGGGGCAG 
GAGGAGGAAG AGGGGCAGGT GGAGGAGGAG GAGGGGGACG TGTCTGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCTTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCCTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGGG AGCCCGAGAC GCCTCGGACC 
CACTACATCC GCATCCACAC GCTGCTGCAC AGCCTGCCCT CCGCGCGGGG CGGCGGCGCG 
GACGAGGACG GCGCGGAGGA GGAGGCCACC CTCAAGGATG CCTCGGAGAA GGAGGCGCTC 
AGCGAGGTGG ACACGGTCGC CGCCGAGCCT CCTCCCCTGG AAGAGGATGG AGAGGCCCCC 
GAGGGGGCCA CTCCAGGCAC AGCGCCCCCG GGCCACTCGG GCTTGGCCAA CGGCTCGGTC 
CCAGATGGCG ACACGCTCCC CAGCACGGGG AGCGAGAGCG ACTCCAGCCC GCGGCAGGGC 
GGGGACCACA GCTGCGAGGG CTGCGACGCG TCCTGCTGCA GCCCCTCGTG CTACAGCTCC 
TCGTGCTACA GCACCTCGTG CTACAGCAGC TCCTGCTACA GCCCCTCCTG CTACAACGGC 
AGCAGGTTCG CCAGCCACAC CCGCTTCTCC TCCGTGGACA GCGCCAAGAT CTCCGAGAGC 
ACCGTCTTCT CCTCCCAGGA TGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCGGTG 
CCGGACTCGG TGCAGAGCCC GGAGCTGGAG CCGGAGTCGG CGAACGGCGC GGGGTGGCAA 
GACGAGTCGG CTGCCCCCAC CGGGAGCGCG GCCAGAACCG TGGAAGGTCT GGAGTCCCCC 
GTGGCAGGGC CGAGCAGTCG GAGAGAAGGT GAATGTCCTA TACTCCATAA TTCCCAGCCA 
GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA CATCATCACT ACCCAACAAT CGATGAGCCT 
CTTCCACCAA ACTGGGAAGC TCGCGTTGAC AGCCACGGGC GGGTCTTTTA TGTGGACCAC 
GTGAACCGCA CGACCACCTG GCAGCGTCCC ACATCGGCAG CCACCCCCGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGAAA CTCAACAGGC GGTACCAAAA TATTCAGCGA 
ACCATTGCTA CAGAGAGGCC TGAAGAAGAT TCTGGCAGCC AAAGTTGTGA GCAAGTCCCA 
GCAGGAGGCA GCGGAGGAGG TGGCGGGAGT GATTCAGAGG CTGAAGCTTC TCAGTCGAGC 
ATAGATCTGA GGAGAGATGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCTC CAGCTGTCAA GTTCATCACC AACCCTGAGT TCTTCACTGT ACTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TGAAGCACAT GATCCTCAAG 
GTCCGGCGGG ACGCGCGCAA CTTTGAGCGC TACCAACACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TTGCGGACGC TCGGCTGGAG TTGCCCCGGG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AACCACCTGA CACATCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCACTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGTTTG GCGAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACCGAGGG TAATCATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ATGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTACAGCTT CTCTCCTCGC TGTTCACCCT GCTCGTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCCT ACAGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TCATTCGGCG AGACCACCTG TTGGAGGGAA CCTTCAATCA GGTTATGGCC 
TATTCCCGGA AGGAGCTCCA GCGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC GCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTCGA GTACTCCGCC AATGACACCT ACACCGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCATCTGGAA TGGTTCAGGT TTAGTGGTCG AATCCTAGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTGGAC GCTTTCTTCA CAAGGCCCTT CTATAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATC TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TCGACCTCAC ATTCACGGTT 
AATGAAGAAG TTTTTGGCCA GGTAACGGAG AGGGAGTTAA AGTCTGGAGG AGCGAACACC 
CAAGTGACAG AAAAGAACAA GAAAGAGTAC ATCGAGAGGA TGGTGAGGTG GCGGGTGGAG 
CGCGGAGCGG TGCAGCAGAC CGAGGCCCTG GTCCGGGGCT TCTACGAGGT GGTGGACTCG 
AGGCTGGTCT CCGTGTTCGA TGCCAGGGAG CTGGAGCTGG TGATCGCGGG CACGGCGGAG 
ATCGACCTGG CGGACTGGAG GAACAACGCC GAGTACCGGG GAGGTTATCA CGACGGGCAT 
CTCGTGATCC GCTGGTTCTG GGCTGCGGTG GAACGTTTCA ACAATGAGCA GAGACTCAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTTGC AGCCCTTCGA 
GGAAGCAACG GGCTTCGGCG TTTTTGCATA GAGAAATGGG GGAAAATTAC ATCTCTCCCC 
AGGGCACACA CGTGCTTCAA TCGATTGGAT CTTCCACCTT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACGGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007125025.1
CDS	58..4791
Translation	MASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSCYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVTGSHRGQILWKIDASSYFVDPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSRIAGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNSLVASSRGEPPCTNAPEPSGTSRPEQLSQGSAVGPAGNESLELASPVEAAAGTTEAGDDGTVCVRPEGAGELLAPWPEDTEPALSAEELAERLDLGGEAALPGLLEDGRAPGSPGQPVAKEVATESRAGGQEEEEGQVEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSARGGGADEDGAEEEATLKDASEKEALSEVDTVAAEPPPLEEDGEAPEGATPGTAPPGHSGLANGSVPDGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDDEEEENSAFESVPDSVQSPELEPESANGAGWQDESAAPTGSAARTVEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARVDSHGRVFYVDHVNRTTTWQRPTSAATPDGMRRSGSIQQMEKLNRRYQNIQRTIATERPEEDSGSQSCEQVPAGGSGGGGGSDSEAEASQSSIDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADARLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGAVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNAEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007124963.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007124963.1

ORF Insert Sequence:

ATGGCATCTC CATCCAGAAA CTCCCAGAAC CGACGCCGGT GCAAGGAGCC CCTCCGCCAC 
AGCTACAACC CGGGCCAGTT CCACAGCATG GCCATTAGGA CCGGCCCCCA TGGCGCTGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACTCTC 
ATGGTCAGCA GCTCCTGCTA CAGCATAGGG CACTCGCAGG ACCTGGTGAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATCGA TGAGGTCTTG 
TCGGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCACTG GTTCTCATCG GGGCCAGATC 
CTCTGGAAGA TCGATGCCAG CTCTTACTTT GTGGACCCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCCTTGCGA GCCACCACCC CCAGTGTCAC CGTCAAAAAC 
TCAGCAGCTC CTATTTTTAA AAGTATTGGC TCTGATGAGA CTGTACAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGGAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCACGG ACAGGAGAGG CGATCCAGGA TTGCCGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAGAGACT CCTGGAAAGA CATGCCATAG GGGACAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGACGATGAG GAGATTTCCC TGAGTACCGA ACCTGAGTCT 
GCAGAAATTC AGGACAGCCC CATGAATAGC CTGGTGGCAA GCAGCCGTGG GGAGCCCCCT 
TGCACTAATG CACCAGAGCC CTCGGGGACC TCAAGGCCGG AGCAGCTGAG CCAGGGCAGC 
GCGGTGGGCC CTGCAGGGAA CGAGAGCCTG GAGCTTGCCA GTCCGGTTGA GGCCGCCGCA 
GGTACCACGG AGGCAGGAGA TGATGGCACG GTCTGTGTGA GGCCTGAGGG GGCTGGGGAG 
CTTCTGGCCC CCTGGCCAGA GGACACGGAG CCCGCCCTGA GCGCGGAAGA ACTGGCCGAG 
CGGCTGGACC TGGGCGGGGA GGCGGCCCTA CCCGGGCTGC TGGAGGACGG TCGAGCCCCG 
GGCAGCCCCG GGCAGCCAGT GGCCAAGGAG GTGGCGACAG AGAGCCGGGC TGGGGGGCAG 
GAGGAGGAAG AGGGGCAGGT GGAGGAGGAG GAGGGGGACG TGTCTGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCTTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCCTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGGG AGCCCGAGAC GCCTCGGACC 
CACTACATCC GCATCCACAC GCTGCTGCAC AGCCTGCCCT CCGCGCGGGG CGGCGGCGCG 
GACGAGGACG GCGCGGAGGA GGAGGCCACC CTCAAGGATG CCTCGGAGAA GGAGGCGCTC 
AGCGAGGTGG ACACGGTCGC CGCCGAGCCT CCTCCCCTGG AAGAGGATGG AGAGGCCCCC 
GAGGGGGCCA CTCCAGGCAC AGCGCCCCCG GGCCACTCGG GCTTGGCCAA CGGCTCGGTC 
CCAGATGGCG ACACGCTCCC CAGCACGGGG AGCGAGAGCG ACTCCAGCCC GCGGCAGGGC 
GGGGACCACA GCTGCGAGGG CTGCGACGCG TCCTGCTGCA GCCCCTCGTG CTACAGCTCC 
TCGTGCTACA GCACCTCGTG CTACAGCAGC TCCTGCTACA GCCCCTCCTG CTACAACGGC 
AGCAGGTTCG CCAGCCACAC CCGCTTCTCC TCCGTGGACA GCGCCAAGAT CTCCGAGAGC 
ACCGTCTTCT CCTCCCAGGA TGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCGGTG 
CCGGACTCGG TGCAGAGCCC GGAGCTGGAG CCGGAGTCGG CGAACGGCGC GGGGTGGCAA 
GACGAGTCGG CTGCCCCCAC CGGGAGCGCG GCCAGAACCG TGGAAGGTCT GGAGTCCCCC 
GTGGCAGGGC CGAGCAGTCG GAGAGAAGGT GAATGTCCTA TACTCCATAA TTCCCAGCCA 
GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA CATCATCACT ACCCAACAAT CGATGAGCCT 
CTTCCACCAA ACTGGGAAGC TCGCGTTGAC AGCCACGGGC GGGTCTTTTA TGTGGACCAC 
GTGAACCGCA CGACCACCTG GCAGCGTCCC ACATCGGCAG CCACCCCCGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGAAA CTCAACAGGC GGTACCAAAA TATTCAGCGA 
ACCATTGCTA CAGAGAGGCC TGAAGAAGAT TCTGGCAGCC AAAGTTGTGA GCAAGTCCCA 
GCAGGAGGCA GCGGAGGAGG TGGCGGGAGT GATTCAGAGG CTGAAGCTTC TCAGTCGAGC 
ATAGATCTGA GGAGAGATGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCTC CAGCTGTCAA GTTCATCACC AACCCTGAGT TCTTCACTGT ACTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TGAAGCACAT GATCCTCAAG 
GTCCGGCGGG ACGCGCGCAA CTTTGAGCGC TACCAACACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TTGCGGACGC TCGGCTGGAG TTGCCCCGGG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AACCACCTGA CACATCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCACTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGTTTG GCGAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACCGAGGG TAATCATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ATGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTACAGCTT CTCTCCTCGC TGTTCACCCT GCTCGTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCCT ACAGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TCATTCGGCG AGACCACCTG TTGGAGGGAA CCTTCAATCA GGTTATGGCC 
TATTCCCGGA AGGAGCTCCA GCGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC GCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTCGA GTACTCCGCC AATGACACCT ACACCGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCATCTGGAA TGGTTCAGGT TTAGTGGTCG AATCCTAGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTGGAC GCTTTCTTCA CAAGGCCCTT CTATAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATC TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TCGACCTCAC ATTCACGGTT 
AATGAAGAAG TTTTTGGCCA GGTAACGGAG AGGGAGTTAA AGTCTGGAGG AGCGAACACC 
CAAGTGACAG AAAAGAACAA GAAAGAGTAC ATCGAGAGGA TGGTGAGGTG GCGGGTGGAG 
CGCGGAGCGG TGCAGCAGAC CGAGGCCCTG GTCCGGGGCT TCTACGAGGT GGTGGACTCG 
AGGCTGGTCT CCGTGTTCGA TGCCAGGGAG CTGGAGCTGG TGATCGCGGG CACGGCGGAG 
ATCGACCTGG CGGACTGGAG GAACAACGCC GAGTACCGGG GAGGTTATCA CGACGGGCAT 
CTCGTGATCC GCTGGTTCTG GGCTGCGGTG GAACGTTTCA ACAATGAGCA GAGACTCAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTTGC AGCCCTTCGA 
GGAAGCAACG GGCTTCGGCG TTTTTGCATA GAGAAATGGG GGAAAATTAC ATCTCTCCCC 
AGGGCACACA CGTGCTTCAA TCGATTGGAT CTTCCACCTT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACGGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_007124963.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007125025.1 Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Price & Availability↑

CloneID	OPh304051
Clone ID Related Accession (Same CDS sequence)	XM_007124963.2
Accession Version	XM_007124963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041218.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007124963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CATCCAGAAA CTCCCAGAAC CGACGCCGGT GCAAGGAGCC CCTCCGCCAC 
AGCTACAACC CGGGCCAGTT CCACAGCATG GCCATTAGGA CCGGCCCCCA TGGCGCTGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACTCTC 
ATGGTCAGCA GCTCCTGCTA CAGCATAGGG CACTCGCAGG ACCTGGTGAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATCGA TGAGGTCTTG 
TCGGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCACTG GTTCTCATCG GGGCCAGATC 
CTCTGGAAGA TCGATGCCAG CTCTTACTTT GTGGACCCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCCTTGCGA GCCACCACCC CCAGTGTCAC CGTCAAAAAC 
TCAGCAGCTC CTATTTTTAA AAGTATTGGC TCTGATGAGA CCGTACAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGGAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCACGG ACAGGAGAGG CGATCCAGGA TTGCCGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAGAGACT CCTGGAAAGA CATGCCATAG GGGACAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGACGATGAG GAGATTTCCC TGAGTACCGA ACCTGAGTCT 
GCAGAAATTC AGGACAGCCC CATGAATAGC CTGGTGGCAA GCAGCCGTGG GGAGCCCCCT 
TGCACTAATG CACCAGAGCC CTCGGGGACC TCAAGGCCGG AGCAGCTGAG CCAGGGCAGC 
GCGGTGGGCC CTGCAGGGAA CGAGAGCCTG GAGCTTGCCA GTCCGGTTGA GGCCGCCGCA 
GGTACCACGG AGGCAGGAGA TGATGGCACG GTCTGTGTGA GGCCTGAGGG GGCTGGGGAG 
CTTCTGGCCC CCTGGCCAGA GGACACGGAG CCCGCCCTGA GCGCGGAAGA ACTGGCCGAG 
CGGCTGGACC TGGGCGGGGA GGCGGCCCTA CCCGGGCTGC TGGAGGACGG TCGAGCCCCG 
GGCAGCCCCG GGCAGCCAGT GGCCAAGGAG GTGGCGACAG AGAGCCGGGC TGGGGGGCAG 
GAGGAGGAAG AGGGGCAGGT GGAGGAGGAG GAGGGGGACG TGTCTGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCTTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCCTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGGG AGCCCGAGAC GCCTCGGACC 
CACTACATCC GCATCCACAC GCTGCTGCAC AGCCTGCCCT CCGCGCGGGG CGGCGGCGCG 
GACGAGGACG GCGCGGAGGA GGAGGCCACC CTCAAGGATG CCTCGGAGAA GGAGGCGCTC 
AGCGAGGTGG ACACGGTCGC CGCCGAGCCT CCTCCCCTGG AAGAGGATGG AGAGGCCCCC 
GAGGGGGCCA CTCCAGGCAC AGCGCCCCCG GGCCACTCGG GCTTGGCCAA CGGCTCGGTC 
CCAGATGGCG ACACGCTCCC CAGCACGGGG AGCGAGAGCG ACTCCAGCCC GCGGCAGGGC 
GGGGACCACA GCTGCGAGGG CTGCGACGCG TCCTGCTGCA GCCCCTCGTG CTACAGCTCC 
TCGTGCTACA GCACCTCGTG CTACAGCAGC TCCTGCTACA GCCCCTCCTG CTACAACGGC 
AGCAGGTTCG CCAGCCACAC CCGCTTCTCC TCCGTGGACA GCGCCAAGAT CTCCGAGAGC 
ACCGTCTTCT CCTCCCAGGA TGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCGGTG 
CCGGACTCGG TGCAGAGCCC GGAGCTGGAG CCGGAGTCGG CGAACGGCGC GGGGTGGCAA 
GACGAGTCGG CTGCCCCCAC CGGGAGCGCG GCCAGAACCG TGGAAGGTCT GGAGTCCCCC 
GTGGCAGGGC CGAGCAGTCG GAGAGAAGGT GAATGTCCTA TACTCCATAA TTCCCAGCCA 
GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA CATCATCACT ACCCAACAAT CGATGAGCCT 
CTTCCACCAA ACTGGGAAGC TCGCGTTGAC AGCCACGGGC GGGTCTTTTA TGTGGACCAC 
GTGAACCGCA CGACCACCTG GCAGCGTCCC ACATCGGCAG CCACCCCCGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGAAA CTCAACAGGC GGTACCAAAA TATTCAGCGA 
ACCATTGCTA CAGAGAGGCC TGAAGAAGAT TCTGGCAGCC AAAGTTGTGA GCAAGTCCCA 
GCAGGAGGCA GCGGAGGAGG TGGCGGGAGT GATTCAGAGG CTGAAGCTTC TCAGTCGAGC 
ATAGATCTGA GGAGAGATGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCTC CAGCTGTCAA GTTCATCACC AACCCTGAGT TCTTCACTGT ACTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TGAAGCACAT GATCCTCAAG 
GTCCGGCGGG ACGCGCGCAA CTTTGAGCGC TACCAACACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TTGCGGACGC TCGGCTGGAG TTGCCCCGGG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AACCACCTGA CACATCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCACTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGTTTG GCGAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACCGAGGG TAATCATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ATGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTACAGCTT CTCTCCTCGC TGTTCACCCT GCTCGTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCCT ACAGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TCATTCGGCG AGACCACCTG TTGGAGGGAA CCTTCAATCA GGTTATGGCC 
TATTCCCGGA AGGAGCTCCA GCGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC GCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTCGA GTACTCCGCC AATGACACCT ACACCGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCATCTGGAA TGGTTCAGGT TTAGTGGTCG AATCCTAGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTGGAC GCTTTCTTCA CAAGGCCCTT CTATAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATC TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TCGACCTCAC ATTCACGGTT 
AATGAAGAAG TTTTTGGCCA GGTAACGGAG AGGGAGTTAA AGTCTGGAGG AGCGAACACC 
CAAGTGACAG AAAAGAACAA GAAAGAGTAC ATCGAGAGGA TGGTGAGGTG GCGGGTGGAG 
CGCGGAGCGG TGCAGCAGAC CGAGGCCCTG GTCCGGGGCT TCTACGAGGT GGTGGACTCG 
AGGCTGGTCT CCGTGTTCGA TGCCAGGGAG CTGGAGCTGG TGATCGCGGG CACGGCGGAG 
ATCGACCTGG CGGACTGGAG GAACAACGCC GAGTACCGGG GAGGTTATCA CGACGGGCAT 
CTCGTGATCC GCTGGTTCTG GGCTGCGGTG GAACGTTTCA ACAATGAGCA GAGACTCAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTTGC AGCCCTTCGA 
GGAAGCAACG GGCTTCGGCG TTTTTGCATA GAGAAATGGG GGAAAATTAC ATCTCTCCCC 
AGGGCACACA CGTGCTTCAA TCGATTGGAT CTTCCACCTT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACGGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007125025.1
CDS	31..4764
Translation	MASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSCYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVTGSHRGQILWKIDASSYFVDPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSRIAGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNSLVASSRGEPPCTNAPEPSGTSRPEQLSQGSAVGPAGNESLELASPVEAAAGTTEAGDDGTVCVRPEGAGELLAPWPEDTEPALSAEELAERLDLGGEAALPGLLEDGRAPGSPGQPVAKEVATESRAGGQEEEEGQVEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSARGGGADEDGAEEEATLKDASEKEALSEVDTVAAEPPPLEEDGEAPEGATPGTAPPGHSGLANGSVPDGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDDEEEENSAFESVPDSVQSPELEPESANGAGWQDESAAPTGSAARTVEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARVDSHGRVFYVDHVNRTTTWQRPTSAATPDGMRRSGSIQQMEKLNRRYQNIQRTIATERPEEDSGSQSCEQVPAGGSGGGGGSDSEAEASQSSIDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADARLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGAVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNAEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007124963.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007124963.2

ORF Insert Sequence:

ATGGCATCTC CATCCAGAAA CTCCCAGAAC CGACGCCGGT GCAAGGAGCC CCTCCGCCAC 
AGCTACAACC CGGGCCAGTT CCACAGCATG GCCATTAGGA CCGGCCCCCA TGGCGCTGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACTCTC 
ATGGTCAGCA GCTCCTGCTA CAGCATAGGG CACTCGCAGG ACCTGGTGAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATCGA TGAGGTCTTG 
TCGGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCACTG GTTCTCATCG GGGCCAGATC 
CTCTGGAAGA TCGATGCCAG CTCTTACTTT GTGGACCCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCCTTGCGA GCCACCACCC CCAGTGTCAC CGTCAAAAAC 
TCAGCAGCTC CTATTTTTAA AAGTATTGGC TCTGATGAGA CCGTACAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGGAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCACGG ACAGGAGAGG CGATCCAGGA TTGCCGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAGAGACT CCTGGAAAGA CATGCCATAG GGGACAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGACGATGAG GAGATTTCCC TGAGTACCGA ACCTGAGTCT 
GCAGAAATTC AGGACAGCCC CATGAATAGC CTGGTGGCAA GCAGCCGTGG GGAGCCCCCT 
TGCACTAATG CACCAGAGCC CTCGGGGACC TCAAGGCCGG AGCAGCTGAG CCAGGGCAGC 
GCGGTGGGCC CTGCAGGGAA CGAGAGCCTG GAGCTTGCCA GTCCGGTTGA GGCCGCCGCA 
GGTACCACGG AGGCAGGAGA TGATGGCACG GTCTGTGTGA GGCCTGAGGG GGCTGGGGAG 
CTTCTGGCCC CCTGGCCAGA GGACACGGAG CCCGCCCTGA GCGCGGAAGA ACTGGCCGAG 
CGGCTGGACC TGGGCGGGGA GGCGGCCCTA CCCGGGCTGC TGGAGGACGG TCGAGCCCCG 
GGCAGCCCCG GGCAGCCAGT GGCCAAGGAG GTGGCGACAG AGAGCCGGGC TGGGGGGCAG 
GAGGAGGAAG AGGGGCAGGT GGAGGAGGAG GAGGGGGACG TGTCTGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCTTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCCTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGGG AGCCCGAGAC GCCTCGGACC 
CACTACATCC GCATCCACAC GCTGCTGCAC AGCCTGCCCT CCGCGCGGGG CGGCGGCGCG 
GACGAGGACG GCGCGGAGGA GGAGGCCACC CTCAAGGATG CCTCGGAGAA GGAGGCGCTC 
AGCGAGGTGG ACACGGTCGC CGCCGAGCCT CCTCCCCTGG AAGAGGATGG AGAGGCCCCC 
GAGGGGGCCA CTCCAGGCAC AGCGCCCCCG GGCCACTCGG GCTTGGCCAA CGGCTCGGTC 
CCAGATGGCG ACACGCTCCC CAGCACGGGG AGCGAGAGCG ACTCCAGCCC GCGGCAGGGC 
GGGGACCACA GCTGCGAGGG CTGCGACGCG TCCTGCTGCA GCCCCTCGTG CTACAGCTCC 
TCGTGCTACA GCACCTCGTG CTACAGCAGC TCCTGCTACA GCCCCTCCTG CTACAACGGC 
AGCAGGTTCG CCAGCCACAC CCGCTTCTCC TCCGTGGACA GCGCCAAGAT CTCCGAGAGC 
ACCGTCTTCT CCTCCCAGGA TGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCGGTG 
CCGGACTCGG TGCAGAGCCC GGAGCTGGAG CCGGAGTCGG CGAACGGCGC GGGGTGGCAA 
GACGAGTCGG CTGCCCCCAC CGGGAGCGCG GCCAGAACCG TGGAAGGTCT GGAGTCCCCC 
GTGGCAGGGC CGAGCAGTCG GAGAGAAGGT GAATGTCCTA TACTCCATAA TTCCCAGCCA 
GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA CATCATCACT ACCCAACAAT CGATGAGCCT 
CTTCCACCAA ACTGGGAAGC TCGCGTTGAC AGCCACGGGC GGGTCTTTTA TGTGGACCAC 
GTGAACCGCA CGACCACCTG GCAGCGTCCC ACATCGGCAG CCACCCCCGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGAAA CTCAACAGGC GGTACCAAAA TATTCAGCGA 
ACCATTGCTA CAGAGAGGCC TGAAGAAGAT TCTGGCAGCC AAAGTTGTGA GCAAGTCCCA 
GCAGGAGGCA GCGGAGGAGG TGGCGGGAGT GATTCAGAGG CTGAAGCTTC TCAGTCGAGC 
ATAGATCTGA GGAGAGATGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCTC CAGCTGTCAA GTTCATCACC AACCCTGAGT TCTTCACTGT ACTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TGAAGCACAT GATCCTCAAG 
GTCCGGCGGG ACGCGCGCAA CTTTGAGCGC TACCAACACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TTGCGGACGC TCGGCTGGAG TTGCCCCGGG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AACCACCTGA CACATCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCACTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGTTTG GCGAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACCGAGGG TAATCATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ATGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTACAGCTT CTCTCCTCGC TGTTCACCCT GCTCGTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCCT ACAGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TCATTCGGCG AGACCACCTG TTGGAGGGAA CCTTCAATCA GGTTATGGCC 
TATTCCCGGA AGGAGCTCCA GCGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC GCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTCGA GTACTCCGCC AATGACACCT ACACCGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCATCTGGAA TGGTTCAGGT TTAGTGGTCG AATCCTAGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTGGAC GCTTTCTTCA CAAGGCCCTT CTATAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATC TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TCGACCTCAC ATTCACGGTT 
AATGAAGAAG TTTTTGGCCA GGTAACGGAG AGGGAGTTAA AGTCTGGAGG AGCGAACACC 
CAAGTGACAG AAAAGAACAA GAAAGAGTAC ATCGAGAGGA TGGTGAGGTG GCGGGTGGAG 
CGCGGAGCGG TGCAGCAGAC CGAGGCCCTG GTCCGGGGCT TCTACGAGGT GGTGGACTCG 
AGGCTGGTCT CCGTGTTCGA TGCCAGGGAG CTGGAGCTGG TGATCGCGGG CACGGCGGAG 
ATCGACCTGG CGGACTGGAG GAACAACGCC GAGTACCGGG GAGGTTATCA CGACGGGCAT 
CTCGTGATCC GCTGGTTCTG GGCTGCGGTG GAACGTTTCA ACAATGAGCA GAGACTCAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTTGC AGCCCTTCGA 
GGAAGCAACG GGCTTCGGCG TTTTTGCATA GAGAAATGGG GGAAAATTAC ATCTCTCCCC 
AGGGCACACA CGTGCTTCAA TCGATTGGAT CTTCCACCTT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACGGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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