PHATRDRAFT_43159 cDNA ORF clone, Phaeodactylum tricornutum CCAP 1055/1

Gene
Clones
gRNAs

Summary

Gene Symbol	PHATRDRAFT_43159
Entrez Gene ID	7196914
Full Name	predicted protein
Gene Type	protein-coding
Organism	Phaeodactylum tricornutum CCAP 1055/1

mRNA and Protein(s)

mRNA	Protein	Name
XM_002176894.1	XP_002176930.1	predicted protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Phaeodactylum genome reveals the evolutionary history of diatom genomes.
Bowler C, Allen AE, Badger JH, Grimwood J, Jabbari K, Kuo A, Maheswari U, Martens C, Maumus F, Otillar RP, Rayko E, Salamov A, Vandepoele K, Beszteri B, Gruber A, Heijde M, Katinka M, Mock T, Valentin K, Verret F, Berges JA, Brownlee C, Cadoret JP, Chiovitti A, Choi CJ, Coesel S, De Martino A, Detter JC, Durkin C, Falciatore A, Fournet J, Haruta M, Huysman MJ, Jenkins BD, Jiroutova K, Jorgensen RE, Joubert Y, Kaplan A, Kröger N, Kroth PG, La Roche J, Lindquist E, Lommer M, Martin-Jézéquel V, Lopez PJ, Lucas S, Mangogna M, McGinnis K, Medlin LK, Montsant A, Oudot-Le Secq MP, Napoli C, Obornik M, Parker MS, Petit JL, Porcel BM, Poulsen N, Robison M, Rychlewski L, Rynearson TA, Schmutz J, Shapiro H, Siaut M, Stanley M, Sussman MR, Taylor AR, Vardi A, von Dassow P, Vyverman W, Willis A, Wyrwicz LS, Rokhsar DS, Weissenbach J, Armbrust EV, Green BR, Van de Peer Y, Grigoriev IV
Nature456(7219)239-44(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHATRDRAFT_43159 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHATRDRAFT_43159 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPb06288	XM_002176894.1 Latest version!	Phaeodactylum tricornutum CCAP 1055/1 predicted protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHATRDRAFT_43159 ( XM_002176894.1 ) cDNA ORF clone, Phaeodactylum tricornutum CCAP 1055/1 -> XP_002176930.1 Phaeodactylum tricornutum CCAP 1055/1 predicted protein partial mRNA.

Price & Availability↑

CloneID	OPb06288
Clone ID Related Accession (Same CDS sequence)	XM_002176894.1
Accession Version	XM_002176894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Phaeodactylum tricornutum CCAP 1055/1
Product	predicted protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_011669). COMPLETENESS: incomplete on the 5' end.

ATGGACGCAT GCCAGACGCT CTGGGCTTTC CCTTTGCAGC CCACATTGGC GAAAGCGTGG 
GTGTGGGTGT GGGGGTCTGG ACCGCAACCG CAACCTCCTG GAGAGAATCT CTGTGGGTTC 
CCGTTGCGCC GTGCCCACGT CCTTGCGTGT TGGACGTTGT TTCCCGGTAT GGTAGCTCGA 
AGCATTGCGA CTTTCCACTA TTACTACCAA CCACCAACTA CTTCTGCTAC TCGTACGAGC 
ATTAGCATTA GCACTAACGT TGCGCACCGG ACGACCGCTA CGGATACGTT TCGTTACGTT 
ACGTTAATTT TCTACCACCG CCACCACCAC CACTACGAAC GATACGATAC GATACAATTT 
CATACAATAC AATCCACTAC GATCGTGACA ATCCCTCCCG TAAGTCGTGT CATGGAGTCG 
ACGGCAGCCA ACGAGACGTC CGTGAAAGTC GTCGTACAGG ACCAACACGA AGAAGTGCTC 
GAACCACTCG AGTCATTGGA AGTGGACCTT GACAACGATG AGTATGCCGA CATTATCGAC 
GGCGATGATT CGGTCCAGCC AGAATCCTTC CCGCCTCCGA CACCAACAAC ACGCCAGGCA 
CTGAATGATT TCGAACCCAC CAACAGAGAG TCGGACGATG ACAACAACCA CAACAACAAT 
ACCGAAAGCC ATACTCGTAC CGATCACCAC AATGCAAAGG CGGGTGCTTC AGAGACGGAA 
CCGGCCGGAG AAGATGATCG GCAGCTCTCG GCATACGAAC GCTTACGACT GGAACGTATT 
CGCCGCAATC AACAAAAATT GGCCCAACTC GGACTCGAAG GAAGAACCAG CGCCGCCGGA 
GGCTGGGGTC CACGTTCGCG TCCCAAAAAG CCCAAACTCT TGCGGAAAAA GGCCGACGAA 
CCCGCACAGC AACGATCCAG TCTATCGCGA CGCACCAAAA CTGAACCCAA AAATTACGCC 
GAACCGCCAA ACTCCGTATC GGCCTTGTTG CGCAAACAAG GCGAAGAAGC GGCCGCTGCG 
AAAAAGGATC AACCCTTGGA GAAGAAAAAA ACCAAGGATC TGAGTGAACG GATGGAAAGA 
TTTGTGCACT TGGAGTTCAA GCGCATCCGT GGTCACAAAA CACTCGTACA CAAACAGGCG 
AAACGAGACC TCCGCCAGGC CGAACGGGAA CTGAAGTACT GGAAGTCCAT CGCGAATAAG 
CAAAAACGCC GAGAAATGCG CAAAGCGGAA AATAGACAAA GTCTCAAAAC GAAACAGGAA 
GAAGAACGAC GGCTCTTGGG TGGAACGATC AAGGAAGTTT TACAACAAAT CGACGCGCGG 
ATGCCCGAAC TCGTCCAGGC GGCTGCGGAA TACGACGACA AATATGAGCA CAAACCGAAC 
GGGACCCGCA AATTGGTTGC CGATCAAGTC TTTAACGCTG TGTTTTTTTT TTTTGCGCAG 
TCCGCAGTAT CGCTCTTGAA CACGACATTA CTGGACCGGG CTCCACGAGA GCCAGCACCG 
ACCCGTCGAA GTCGTAGATC CACTGCCGAG GAAATCGTCA CACCCCTAAA GTTAGCACAG 
AATCAATTTA CTGCCAATTC TCCGGAAGAA GAGGATACGT ACCGAAACGT TGCCGACAAA 
GAAGCAATAG CCCCGGAGGA CGATGAAACC TCTAACGGAA GAGATCGGCC TGGTACGAAA 
CGGAAACGAA ATGCTCGGAA TGTTGGTGGC TGGGTTTCTC CTCAATTTGC GTTCGAGATT 
GATAGATCAT GGCTTGACCA GGACAACACA AAAGCGCACT TTGATTTGTA TTCATACGTA 
CCACAAACTG GGGATACTGT GTTATACTAT CCAGCCGGGC ATCGCGCATA TGTTAAAGAC 
TATCCCGATA TCCTGGGAAA AAAGACGAGG CAGATTACTA GACTTCCTCT TTGGGAACGC 
GCGGTCATTG AGCGGGACAA AATAGACAGA GGCGAGGGGC CCAAAACCGG TGACAGTTCC 
TTGAATGTTT GGTGGACGGA TGAGTGGTTG GAGGGCATCG AAGGTGACCT GGGAAAATAT 
CCAATACTAT GTCGAGTTCA CGCCACAAAT GCTGAATTTC CGCCGGATCG ATTCACTAAA 
GTAATAAAAA AAGGGGAGAT TTCTTGGAAA GATCCTAAAG AGCAAACCAA AAGAACGAAA 
GAATCATCAA GAACACCTCT ACGACTGGCC GTCACACTAC GGCCGTTGGC ACCGGTTGTT 
CCTCCTCCTA AGGAATCCAG CGGCGATGCC TTGGCCAGCA GTACTCAACC AGATCTGCTC 
CCATCACGTT TCAAGGATGA CCAACCGTTG GCTCTTCCTC CATTCTTCAC AGTTGTGACG 
TTCCCTTCGC AGCAGAAGCC GTTTATAGTC CCTTTTGCAT GGGCTTACTC GATTTCCAAT 
TCGCTTTGCC TGGATCAAAG CGTAGTAGAA AAAGGAAGGG AGGCTTCAAA GTTGCACGTC 
CGTTCCTTTG ATACCCTATC CGACACTTCA TATTCTCGAT TGGATGACAG AATTGAGGCT 
CTGCAAGTAT TCTTAAAAAA ATGCACCAGA GGACCACGCG GAAAATTGAG TTTGCCGACC 
GATTGTTTAG AATCAGCAGG GATGCTGTCG CTAGCAGAAA CAAATGTGTT GCTTGATTTC 
TTCGACCATT TTGTAAAAGA CAGACAGTCT GATGTTTCTG ATACCCAAGC CGTGGTAGGT 
TTTTTTAACT TGGTCAGGTC CACATTGCCT TTATGGAACG GCGTCTCTGT TGTCCACAGC 
CCGGACGAGA AGAAAGACAG GAAGATCTCA CCGTGGGACC TCTCCCTATG GAAGAAACGA 
ATGATGATTC CAGATTATTC GCCAGATAAT TTGTCAGATG CGTCGTTGTC GATGGACGAT 
CGACTGCGGG ATCGGATAGA ACGCGTAATC GACGACATAG CAAAGAATGA CTCAAATGCA 
GAACTTTTCC GGTCTCCTGT AACCGAAGAA GATGCTCCCT CTTATCCCTG CGCCGTACCT 
CTTTCAATTT CTCTTGACAA AATTATTCGT CGCTTGAAAG GGGGAAAATC TGGCAGTCAA 
AGGTGCTATT ATCGGAATAC GGATTCACTT CTGTCGGATG TAAGTTCGCT TTTGGAGAAT 
TGTCTGCTGT ACAACAGTCC AGAATCATCA CTCGTTGAAA CGGCGGTGGC GCTCGTTTCA 
AAAATAAAGG ATGAAATTTC TCGGGTTGCG AAAGACCATT TTAAAGAAGT GATACAAGCA 
AAAAAGGCGG ACGAGGAAAG AAAAAGTCGT ATACTTAGGC ATTGTGAGGC TGTTGATCCT 
GCTGAAAACT GCGGCATCAC GGATAGTCTG AAGGAGCTGT ATGTCATGAG CTCCATAAGG 
AAGCCTTACA AAGATCAAAT CGATTTAGAG TGGCTGCAAC AAACGACAGA GATCGTTGAG 
AATCGAGACA CCGGCGTTTC TACGCCACAT CGAAGCTGGG TGCCGCAAGC TGGCGATGAT 
ATTCTATACT GCCCTTCACT ACATTCAGAA TTTGTCAAAG CTCACTTTAG CTCACTTGAG 
CCCTATCAGT GCATCGTGCA CAATCTGCAA GACTACAACC TAAAAAACAA GAATGAAGCC 
GCTGACGACT CGACCGCTTT AACGAAAGAA GGATTCAGTG ACAAATTTCT AGAAGGATGG 
GTGAAAGGTC GCGTGATTTG GGTACGCAGC TCGTTCCCAA AATATTCAAC CAAGAAGGAT 
GGCGTGGAAG CAAAAACGTT TCCTTCGAAT TCGTCGATTT TGGTGGCGGG GATCAAATTG 
GAGCCTGATT TTGATGACAC CAAGCCTCGG ATTATCTGTT GGAGGCCTTG CATATTTGAT 
TATTCTGTCT GCAAGATTTG TGGTCTATCT ACACAATCGT TTTTCAAGCC TTGCGACGGG 
AACGATGAAG CTTTTGAAGA ATCAAAGCTG TCGCTTTCGC TGATTGAGCT GCAAACGATA 
GATCGGTGTT TCAACTTTCT CAAGAAACGC TGCATTCAAG GAACCGAACC AAGCTACATC 
GACCATCAGT TGACTAGAGC AAATGTTCGA CAAGGCTATC TCCCTCCGTC TGGCCGGTCC 
TCTGCAAAAT CACTTCCCGC TTTTGAGCTT GCTTACAAGG ACGCGCAAAA GGCGAAGGTG 
AAAGGCACAA GAGGGGCAAA AGTATCTGTG TGCCCTGGGA TTCCGGAGGC ATTGATACGG 
CATCTCTTAG ATTTTGGATT TCTTGCTCCT TTTGTACTCT CGTCTGGGAA AAGCAACAAG 
GAAAAGTTAC ACAATGACGG CATGATATCG CCTTGGCCAA AAATGTCTCT AGAATTGATA 
CACTCTCGGG TCAAAAATGG TTTTTATCGC CACCGGGCTG CAATTCTGAA CGATGTCATA 
GAAGCATACT CTTCGGTGGT TTTCCTAGTT CTAGTTGAAG CAGGCAAAAG AAAGCGCTCT 
CTTCTAAGTA TAAGGCGTAT CGCTCGAGCT CTGGCAGCTT CTAAAAGTCA TAGCAATGGA 
GAGGAAGAAA CAGTGATGGT AGATACCAAG ACCATTGATC AAGAAGAAAT TGATCTACTC 
AAACGGATCC GAAAAATTCG AGAGCTATAC GCAACAGCTC TTGTGGTGGT TTCCGATACA 
ATTCACAGCG AAAGGATTTT TGGGTTGGTC GACCGACCAT CTCTGAAGCA TATACCAAAA 
TCTACTGAAC CGGTACTGGT TCAGAGCCCC GAGCAGATAC AGGCTAGGAA GCGGTTGGAA 
TATATCCTAT TGGCTTTGAG TCGAGACCAG CTGCAAAACA CTTTCCCGTC CATCGAATCC 
AACGGGACAA CTTTTCCGAC GATAAAAGTC AAGTTGTCTT TCGGCGGAAA GCTGCTGATG 
ACCAAAATGC GTGTCAGTGT GGATAAGTTT GCCGTGGGCT TTTTGGGTAC CAAAGAAGTG 
AATTTGAAGA TCAGGCTGAA TGGTCGGCAG TTCACGATGG AGAGAGCCGC TCCACAGCTT 
TTGACAGGAA GCTCGGCTCT TAATGACCTG TCAACAAACG ATACAAACAC CCGAGATTCA 
ACGAAAGTAG CGAAAGGCAG AGAATTTAAA CACGTTTTGT GCGATTCTGT TTGTTTCGAC 
CACGAAGACT ACGAAGGAAA AGATAGCCTC GTGAAACTTT TTTTCGGAAG ACCTGGTAGA 
CTGCATCCTT GTGCTAGATG TCAAGCTTAC CGTCGATCTC TACTCAGTTG TCGTGTACGG 
CGTTCACATT CAAATGTGGA TTTCGATTGG GTCGAAAATT TCAGCACTAC CGGTGGACTG 
GAAAAGCTGT TAAAGCAGAT GAACCCCGAA TGGCCCGAAT GGGAAGTCTG TGAGGTTGGA 
AAAAGCTCTA CAGACAACAC TTTAGCATCG GAAAAACCTC TACCGTCTCC TTTAGAAGAA 
ATCGAGAAAG AAGATGGAAA TGATAACGAC GACGGGGATG CCGATGAAGA CGGGGATCCC 
GATGTCGAAG ATGGAACCCA AGAGGTCGAT GAAGAAGAGG AGGAAGACGA GTTGGCGTTG 
GAGTTGGCAG ACCCCCAGCA AATGATTGAG AAAGCTGAGA AGACACTGTG CCTTGCAAAA 
TCTGTTCAGC AAGAGGCTGA CGTATACTAC AAAGCTCCGG TTCGTTTGAG CAAAGCATTT 
GTCGAAACCG CCTTTCCGAT CGACCCAACG GACGGTCACT GCGTATATTG CATTGTGTGT 
GGACTGAGCG GCGACCTGCT ATGCTGTGAC GGATGCGCTA ACGTTGTCCA CGGCAAATGT 
ATTGGATTGA CTTCGTTTCC AGAGGGAGAT TGGTTTTGCG AAGAGTGTCT TCACAAGAAG 
TTGATACAGA ATGGCCTAGT AACCAATCGA CGCCGGGGCA GTAGTGACGC TGCTCCTATC 
AAAGGCAATG GAGACTTAAG GTTAGCAGAG GAGAACGGAC ATTGTCCAAA AACCGCTGTG 
AAGTTTGACG CATTTGGTCG ATTCGAGTTC GACGATGAAC ATGCAGAGAA AGTGTCGACG 
CTTCTAGACG AGCTACGTGC TGGACGGCCC GAGCAACCGC GAAAACGAAA ACCTGCACCT 
GGTCAGTCAT TAGGGTCAAA CAAAATAGCC GGAATACAGC GAAGAGCAGA ATTTTTGCAA 
CAACCTATTG TCTTGGGAGG TGAACTCTCC GGTCTTGTGC CGATGGCACG GAAGTTTTTG 
GGTGCGGTGG GGATTGAAAC TGCAGATGCC TTCCTTTCCA TGCGGACCAC TGACCTTGCC 
AAACGGTTCG CAACATGGCG TCGCAAGACT GGAATGGCAA AATTAAATGG AAGTGGGGAG 
ACGGCATATG TCAGTTCATG GAAAAGCGCG TGTCGGAAAC TTTGTGGCGA TATTGAACAC 
AAGATGGTGT ACCTATCCGA TCCAGGAGAT GACGACGACG ACGGGTCTTA TTCTAAACGA 
AAAGTGCGAC GCCGAAAGAA GGGCAAGACT CGCCCGAACA CAACAGGTAA TCGACGGCGT 
CGCCTTGATC TACAAAGCCT TACGGATCCG TTAGAGGCTT TAAGCACCGT CAACCAACAG 
TTTTTGGCCA CCATTGATAT CTCAACCGCG AAAGAATTTC TCACAACTCG TACGGGGGAC 
ATTGCGGAAG CACTCGTCAA GTGGCGAATA AAAGAAGGTC TTCCGCGCTT GAAAGGAAAC 
GGAGAAGTAG CAACAATCAG TGCGGCAAAG ACATCCTGTC GTAAAATTGC CAGAGAAATG 
GGCTGGACAA AGGTAGCGGA CATTGAATCT GCAGGTCGGG CAGTATTTCA GAGGAAGGAA 
ATACGTGAAG AGTCCAACCC GAGAAAGCGA AAACTCTCCA CCCCACAAGT TGCGACTCGA 
CGGCAAGCGA GTAAAGCTAG TGATGATGTT GAGTCCGACG TGGAAACCGA AAATGAAGCT 
GACGCTCCTA GCCAAGGAGA AAAAAACGGC TTATCAAAGG AGAAGCCGTT GGCGAAACGT 
CGGAAGCGAA ACACCGTCGA ACCAGTCGTT AACACGTCAG TCGCGGTCGT CACTTGCGAA 
AGCACAGCCC GACGCCCTGG TATGCGGACC TCTCGAACCC CCGACCGTTT CGGTGTCGTT 
GCTGCGCCTC CGCAGAAGCG ACGCCGAAAG GTTTCACCCA AAGACGCCGA AGAGCCCAGT 
GAAGTTTTCG ATCTCGAATT CTCCGCTGCC ACCAATGTGC AAAGCTTGCT TTTGAAGCAA 
GTTCCCGGTA GGAGAAGACG TTCCAGGTCC CGAAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002176930.1
CDS	1..7242
Translation	MDACQTLWAFPLQPTLAKAWVWVWGSGPQPQPPGENLCGFPLRRAHVLACWTLFPGMVARSIATFHYYYQPPTTSATRTSISISTNVAHRTTATDTFRYVTLIFYHRHHHHYERYDTIQFHTIQSTTIVTIPPVSRVMESTAANETSVKVVVQDQHEEVLEPLESLEVDLDNDEYADIIDGDDSVQPESFPPPTPTTRQALNDFEPTNRESDDDNNHNNNTESHTRTDHHNAKAGASETEPAGEDDRQLSAYERLRLERIRRNQQKLAQLGLEGRTSAAGGWGPRSRPKKPKLLRKKADEPAQQRSSLSRRTKTEPKNYAEPPNSVSALLRKQGEEAAAAKKDQPLEKKKTKDLSERMERFVHLEFKRIRGHKTLVHKQAKRDLRQAERELKYWKSIANKQKRREMRKAENRQSLKTKQEEERRLLGGTIKEVLQQIDARMPELVQAAAEYDDKYEHKPNGTRKLVADQVFNAVFFFFAQSAVSLLNTTLLDRAPREPAPTRRSRRSTAEEIVTPLKLAQNQFTANSPEEEDTYRNVADKEAIAPEDDETSNGRDRPGTKRKRNARNVGGWVSPQFAFEIDRSWLDQDNTKAHFDLYSYVPQTGDTVLYYPAGHRAYVKDYPDILGKKTRQITRLPLWERAVIERDKIDRGEGPKTGDSSLNVWWTDEWLEGIEGDLGKYPILCRVHATNAEFPPDRFTKVIKKGEISWKDPKEQTKRTKESSRTPLRLAVTLRPLAPVVPPPKESSGDALASSTQPDLLPSRFKDDQPLALPPFFTVVTFPSQQKPFIVPFAWAYSISNSLCLDQSVVEKGREASKLHVRSFDTLSDTSYSRLDDRIEALQVFLKKCTRGPRGKLSLPTDCLESAGMLSLAETNVLLDFFDHFVKDRQSDVSDTQAVVGFFNLVRSTLPLWNGVSVVHSPDEKKDRKISPWDLSLWKKRMMIPDYSPDNLSDASLSMDDRLRDRIERVIDDIAKNDSNAELFRSPVTEEDAPSYPCAVPLSISLDKIIRRLKGGKSGSQRCYYRNTDSLLSDVSSLLENCLLYNSPESSLVETAVALVSKIKDEISRVAKDHFKEVIQAKKADEERKSRILRHCEAVDPAENCGITDSLKELYVMSSIRKPYKDQIDLEWLQQTTEIVENRDTGVSTPHRSWVPQAGDDILYCPSLHSEFVKAHFSSLEPYQCIVHNLQDYNLKNKNEAADDSTALTKEGFSDKFLEGWVKGRVIWVRSSFPKYSTKKDGVEAKTFPSNSSILVAGIKLEPDFDDTKPRIICWRPCIFDYSVCKICGLSTQSFFKPCDGNDEAFEESKLSLSLIELQTIDRCFNFLKKRCIQGTEPSYIDHQLTRANVRQGYLPPSGRSSAKSLPAFELAYKDAQKAKVKGTRGAKVSVCPGIPEALIRHLLDFGFLAPFVLSSGKSNKEKLHNDGMISPWPKMSLELIHSRVKNGFYRHRAAILNDVIEAYSSVVFLVLVEAGKRKRSLLSIRRIARALAASKSHSNGEEETVMVDTKTIDQEEIDLLKRIRKIRELYATALVVVSDTIHSERIFGLVDRPSLKHIPKSTEPVLVQSPEQIQARKRLEYILLALSRDQLQNTFPSIESNGTTFPTIKVKLSFGGKLLMTKMRVSVDKFAVGFLGTKEVNLKIRLNGRQFTMERAAPQLLTGSSALNDLSTNDTNTRDSTKVAKGREFKHVLCDSVCFDHEDYEGKDSLVKLFFGRPGRLHPCARCQAYRRSLLSCRVRRSHSNVDFDWVENFSTTGGLEKLLKQMNPEWPEWEVCEVGKSSTDNTLASEKPLPSPLEEIEKEDGNDNDDGDADEDGDPDVEDGTQEVDEEEEEDELALELADPQQMIEKAEKTLCLAKSVQQEADVYYKAPVRLSKAFVETAFPIDPTDGHCVYCIVCGLSGDLLCCDGCANVVHGKCIGLTSFPEGDWFCEECLHKKLIQNGLVTNRRRGSSDAAPIKGNGDLRLAEENGHCPKTAVKFDAFGRFEFDDEHAEKVSTLLDELRAGRPEQPRKRKPAPGQSLGSNKIAGIQRRAEFLQQPIVLGGELSGLVPMARKFLGAVGIETADAFLSMRTTDLAKRFATWRRKTGMAKLNGSGETAYVSSWKSACRKLCGDIEHKMVYLSDPGDDDDDGSYSKRKVRRRKKGKTRPNTTGNRRRRLDLQSLTDPLEALSTVNQQFLATIDISTAKEFLTTRTGDIAEALVKWRIKEGLPRLKGNGEVATISAAKTSCRKIAREMGWTKVADIESAGRAVFQRKEIREESNPRKRKLSTPQVATRRQASKASDDVESDVETENEADAPSQGEKNGLSKEKPLAKRRKRNTVEPVVNTSVAVVTCESTARRPGMRTSRTPDRFGVVAAPPQKRRRKVSPKDAEEPSEVFDLEFSAATNVQSLLLKQVPGRRRRSRSRKK

Target ORF information:

RefSeq Version	XM_002176894.1
Organism	Phaeodactylum tricornutum CCAP 1055/1
Definition	Phaeodactylum tricornutum CCAP 1055/1 predicted protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002176894.1

ORF Insert Sequence:

ATGGACGCAT GCCAGACGCT CTGGGCTTTC CCTTTGCAGC CCACATTGGC GAAAGCGTGG 
GTGTGGGTGT GGGGGTCTGG ACCGCAACCG CAACCTCCTG GAGAGAATCT CTGTGGGTTC 
CCGTTGCGCC GTGCCCACGT CCTTGCGTGT TGGACGTTGT TTCCCGGTAT GGTAGCTCGA 
AGCATTGCGA CTTTCCACTA TTACTACCAA CCACCAACTA CTTCTGCTAC TCGTACGAGC 
ATTAGCATTA GCACTAACGT TGCGCACCGG ACGACCGCTA CGGATACGTT TCGTTACGTT 
ACGTTAATTT TCTACCACCG CCACCACCAC CACTACGAAC GATACGATAC GATACAATTT 
CATACAATAC AATCCACTAC GATCGTGACA ATCCCTCCCG TAAGTCGTGT CATGGAGTCG 
ACGGCAGCCA ACGAGACGTC CGTGAAAGTC GTCGTACAGG ACCAACACGA AGAAGTGCTC 
GAACCACTCG AGTCATTGGA AGTGGACCTT GACAACGATG AGTATGCCGA CATTATCGAC 
GGCGATGATT CGGTCCAGCC AGAATCCTTC CCGCCTCCGA CACCAACAAC ACGCCAGGCA 
CTGAATGATT TCGAACCCAC CAACAGAGAG TCGGACGATG ACAACAACCA CAACAACAAT 
ACCGAAAGCC ATACTCGTAC CGATCACCAC AATGCAAAGG CGGGTGCTTC AGAGACGGAA 
CCGGCCGGAG AAGATGATCG GCAGCTCTCG GCATACGAAC GCTTACGACT GGAACGTATT 
CGCCGCAATC AACAAAAATT GGCCCAACTC GGACTCGAAG GAAGAACCAG CGCCGCCGGA 
GGCTGGGGTC CACGTTCGCG TCCCAAAAAG CCCAAACTCT TGCGGAAAAA GGCCGACGAA 
CCCGCACAGC AACGATCCAG TCTATCGCGA CGCACCAAAA CTGAACCCAA AAATTACGCC 
GAACCGCCAA ACTCCGTATC GGCCTTGTTG CGCAAACAAG GCGAAGAAGC GGCCGCTGCG 
AAAAAGGATC AACCCTTGGA GAAGAAAAAA ACCAAGGATC TGAGTGAACG GATGGAAAGA 
TTTGTGCACT TGGAGTTCAA GCGCATCCGT GGTCACAAAA CACTCGTACA CAAACAGGCG 
AAACGAGACC TCCGCCAGGC CGAACGGGAA CTGAAGTACT GGAAGTCCAT CGCGAATAAG 
CAAAAACGCC GAGAAATGCG CAAAGCGGAA AATAGACAAA GTCTCAAAAC GAAACAGGAA 
GAAGAACGAC GGCTCTTGGG TGGAACGATC AAGGAAGTTT TACAACAAAT CGACGCGCGG 
ATGCCCGAAC TCGTCCAGGC GGCTGCGGAA TACGACGACA AATATGAGCA CAAACCGAAC 
GGGACCCGCA AATTGGTTGC CGATCAAGTC TTTAACGCTG TGTTTTTTTT TTTTGCGCAG 
TCCGCAGTAT CGCTCTTGAA CACGACATTA CTGGACCGGG CTCCACGAGA GCCAGCACCG 
ACCCGTCGAA GTCGTAGATC CACTGCCGAG GAAATCGTCA CACCCCTAAA GTTAGCACAG 
AATCAATTTA CTGCCAATTC TCCGGAAGAA GAGGATACGT ACCGAAACGT TGCCGACAAA 
GAAGCAATAG CCCCGGAGGA CGATGAAACC TCTAACGGAA GAGATCGGCC TGGTACGAAA 
CGGAAACGAA ATGCTCGGAA TGTTGGTGGC TGGGTTTCTC CTCAATTTGC GTTCGAGATT 
GATAGATCAT GGCTTGACCA GGACAACACA AAAGCGCACT TTGATTTGTA TTCATACGTA 
CCACAAACTG GGGATACTGT GTTATACTAT CCAGCCGGGC ATCGCGCATA TGTTAAAGAC 
TATCCCGATA TCCTGGGAAA AAAGACGAGG CAGATTACTA GACTTCCTCT TTGGGAACGC 
GCGGTCATTG AGCGGGACAA AATAGACAGA GGCGAGGGGC CCAAAACCGG TGACAGTTCC 
TTGAATGTTT GGTGGACGGA TGAGTGGTTG GAGGGCATCG AAGGTGACCT GGGAAAATAT 
CCAATACTAT GTCGAGTTCA CGCCACAAAT GCTGAATTTC CGCCGGATCG ATTCACTAAA 
GTAATAAAAA AAGGGGAGAT TTCTTGGAAA GATCCTAAAG AGCAAACCAA AAGAACGAAA 
GAATCATCAA GAACACCTCT ACGACTGGCC GTCACACTAC GGCCGTTGGC ACCGGTTGTT 
CCTCCTCCTA AGGAATCCAG CGGCGATGCC TTGGCCAGCA GTACTCAACC AGATCTGCTC 
CCATCACGTT TCAAGGATGA CCAACCGTTG GCTCTTCCTC CATTCTTCAC AGTTGTGACG 
TTCCCTTCGC AGCAGAAGCC GTTTATAGTC CCTTTTGCAT GGGCTTACTC GATTTCCAAT 
TCGCTTTGCC TGGATCAAAG CGTAGTAGAA AAAGGAAGGG AGGCTTCAAA GTTGCACGTC 
CGTTCCTTTG ATACCCTATC CGACACTTCA TATTCTCGAT TGGATGACAG AATTGAGGCT 
CTGCAAGTAT TCTTAAAAAA ATGCACCAGA GGACCACGCG GAAAATTGAG TTTGCCGACC 
GATTGTTTAG AATCAGCAGG GATGCTGTCG CTAGCAGAAA CAAATGTGTT GCTTGATTTC 
TTCGACCATT TTGTAAAAGA CAGACAGTCT GATGTTTCTG ATACCCAAGC CGTGGTAGGT 
TTTTTTAACT TGGTCAGGTC CACATTGCCT TTATGGAACG GCGTCTCTGT TGTCCACAGC 
CCGGACGAGA AGAAAGACAG GAAGATCTCA CCGTGGGACC TCTCCCTATG GAAGAAACGA 
ATGATGATTC CAGATTATTC GCCAGATAAT TTGTCAGATG CGTCGTTGTC GATGGACGAT 
CGACTGCGGG ATCGGATAGA ACGCGTAATC GACGACATAG CAAAGAATGA CTCAAATGCA 
GAACTTTTCC GGTCTCCTGT AACCGAAGAA GATGCTCCCT CTTATCCCTG CGCCGTACCT 
CTTTCAATTT CTCTTGACAA AATTATTCGT CGCTTGAAAG GGGGAAAATC TGGCAGTCAA 
AGGTGCTATT ATCGGAATAC GGATTCACTT CTGTCGGATG TAAGTTCGCT TTTGGAGAAT 
TGTCTGCTGT ACAACAGTCC AGAATCATCA CTCGTTGAAA CGGCGGTGGC GCTCGTTTCA 
AAAATAAAGG ATGAAATTTC TCGGGTTGCG AAAGACCATT TTAAAGAAGT GATACAAGCA 
AAAAAGGCGG ACGAGGAAAG AAAAAGTCGT ATACTTAGGC ATTGTGAGGC TGTTGATCCT 
GCTGAAAACT GCGGCATCAC GGATAGTCTG AAGGAGCTGT ATGTCATGAG CTCCATAAGG 
AAGCCTTACA AAGATCAAAT CGATTTAGAG TGGCTGCAAC AAACGACAGA GATCGTTGAG 
AATCGAGACA CCGGCGTTTC TACGCCACAT CGAAGCTGGG TGCCGCAAGC TGGCGATGAT 
ATTCTATACT GCCCTTCACT ACATTCAGAA TTTGTCAAAG CTCACTTTAG CTCACTTGAG 
CCCTATCAGT GCATCGTGCA CAATCTGCAA GACTACAACC TAAAAAACAA GAATGAAGCC 
GCTGACGACT CGACCGCTTT AACGAAAGAA GGATTCAGTG ACAAATTTCT AGAAGGATGG 
GTGAAAGGTC GCGTGATTTG GGTACGCAGC TCGTTCCCAA AATATTCAAC CAAGAAGGAT 
GGCGTGGAAG CAAAAACGTT TCCTTCGAAT TCGTCGATTT TGGTGGCGGG GATCAAATTG 
GAGCCTGATT TTGATGACAC CAAGCCTCGG ATTATCTGTT GGAGGCCTTG CATATTTGAT 
TATTCTGTCT GCAAGATTTG TGGTCTATCT ACACAATCGT TTTTCAAGCC TTGCGACGGG 
AACGATGAAG CTTTTGAAGA ATCAAAGCTG TCGCTTTCGC TGATTGAGCT GCAAACGATA 
GATCGGTGTT TCAACTTTCT CAAGAAACGC TGCATTCAAG GAACCGAACC AAGCTACATC 
GACCATCAGT TGACTAGAGC AAATGTTCGA CAAGGCTATC TCCCTCCGTC TGGCCGGTCC 
TCTGCAAAAT CACTTCCCGC TTTTGAGCTT GCTTACAAGG ACGCGCAAAA GGCGAAGGTG 
AAAGGCACAA GAGGGGCAAA AGTATCTGTG TGCCCTGGGA TTCCGGAGGC ATTGATACGG 
CATCTCTTAG ATTTTGGATT TCTTGCTCCT TTTGTACTCT CGTCTGGGAA AAGCAACAAG 
GAAAAGTTAC ACAATGACGG CATGATATCG CCTTGGCCAA AAATGTCTCT AGAATTGATA 
CACTCTCGGG TCAAAAATGG TTTTTATCGC CACCGGGCTG CAATTCTGAA CGATGTCATA 
GAAGCATACT CTTCGGTGGT TTTCCTAGTT CTAGTTGAAG CAGGCAAAAG AAAGCGCTCT 
CTTCTAAGTA TAAGGCGTAT CGCTCGAGCT CTGGCAGCTT CTAAAAGTCA TAGCAATGGA 
GAGGAAGAAA CAGTGATGGT AGATACCAAG ACCATTGATC AAGAAGAAAT TGATCTACTC 
AAACGGATCC GAAAAATTCG AGAGCTATAC GCAACAGCTC TTGTGGTGGT TTCCGATACA 
ATTCACAGCG AAAGGATTTT TGGGTTGGTC GACCGACCAT CTCTGAAGCA TATACCAAAA 
TCTACTGAAC CGGTACTGGT TCAGAGCCCC GAGCAGATAC AGGCTAGGAA GCGGTTGGAA 
TATATCCTAT TGGCTTTGAG TCGAGACCAG CTGCAAAACA CTTTCCCGTC CATCGAATCC 
AACGGGACAA CTTTTCCGAC GATAAAAGTC AAGTTGTCTT TCGGCGGAAA GCTGCTGATG 
ACCAAAATGC GTGTCAGTGT GGATAAGTTT GCCGTGGGCT TTTTGGGTAC CAAAGAAGTG 
AATTTGAAGA TCAGGCTGAA TGGTCGGCAG TTCACGATGG AGAGAGCCGC TCCACAGCTT 
TTGACAGGAA GCTCGGCTCT TAATGACCTG TCAACAAACG ATACAAACAC CCGAGATTCA 
ACGAAAGTAG CGAAAGGCAG AGAATTTAAA CACGTTTTGT GCGATTCTGT TTGTTTCGAC 
CACGAAGACT ACGAAGGAAA AGATAGCCTC GTGAAACTTT TTTTCGGAAG ACCTGGTAGA 
CTGCATCCTT GTGCTAGATG TCAAGCTTAC CGTCGATCTC TACTCAGTTG TCGTGTACGG 
CGTTCACATT CAAATGTGGA TTTCGATTGG GTCGAAAATT TCAGCACTAC CGGTGGACTG 
GAAAAGCTGT TAAAGCAGAT GAACCCCGAA TGGCCCGAAT GGGAAGTCTG TGAGGTTGGA 
AAAAGCTCTA CAGACAACAC TTTAGCATCG GAAAAACCTC TACCGTCTCC TTTAGAAGAA 
ATCGAGAAAG AAGATGGAAA TGATAACGAC GACGGGGATG CCGATGAAGA CGGGGATCCC 
GATGTCGAAG ATGGAACCCA AGAGGTCGAT GAAGAAGAGG AGGAAGACGA GTTGGCGTTG 
GAGTTGGCAG ACCCCCAGCA AATGATTGAG AAAGCTGAGA AGACACTGTG CCTTGCAAAA 
TCTGTTCAGC AAGAGGCTGA CGTATACTAC AAAGCTCCGG TTCGTTTGAG CAAAGCATTT 
GTCGAAACCG CCTTTCCGAT CGACCCAACG GACGGTCACT GCGTATATTG CATTGTGTGT 
GGACTGAGCG GCGACCTGCT ATGCTGTGAC GGATGCGCTA ACGTTGTCCA CGGCAAATGT 
ATTGGATTGA CTTCGTTTCC AGAGGGAGAT TGGTTTTGCG AAGAGTGTCT TCACAAGAAG 
TTGATACAGA ATGGCCTAGT AACCAATCGA CGCCGGGGCA GTAGTGACGC TGCTCCTATC 
AAAGGCAATG GAGACTTAAG GTTAGCAGAG GAGAACGGAC ATTGTCCAAA AACCGCTGTG 
AAGTTTGACG CATTTGGTCG ATTCGAGTTC GACGATGAAC ATGCAGAGAA AGTGTCGACG 
CTTCTAGACG AGCTACGTGC TGGACGGCCC GAGCAACCGC GAAAACGAAA ACCTGCACCT 
GGTCAGTCAT TAGGGTCAAA CAAAATAGCC GGAATACAGC GAAGAGCAGA ATTTTTGCAA 
CAACCTATTG TCTTGGGAGG TGAACTCTCC GGTCTTGTGC CGATGGCACG GAAGTTTTTG 
GGTGCGGTGG GGATTGAAAC TGCAGATGCC TTCCTTTCCA TGCGGACCAC TGACCTTGCC 
AAACGGTTCG CAACATGGCG TCGCAAGACT GGAATGGCAA AATTAAATGG AAGTGGGGAG 
ACGGCATATG TCAGTTCATG GAAAAGCGCG TGTCGGAAAC TTTGTGGCGA TATTGAACAC 
AAGATGGTGT ACCTATCCGA TCCAGGAGAT GACGACGACG ACGGGTCTTA TTCTAAACGA 
AAAGTGCGAC GCCGAAAGAA GGGCAAGACT CGCCCGAACA CAACAGGTAA TCGACGGCGT 
CGCCTTGATC TACAAAGCCT TACGGATCCG TTAGAGGCTT TAAGCACCGT CAACCAACAG 
TTTTTGGCCA CCATTGATAT CTCAACCGCG AAAGAATTTC TCACAACTCG TACGGGGGAC 
ATTGCGGAAG CACTCGTCAA GTGGCGAATA AAAGAAGGTC TTCCGCGCTT GAAAGGAAAC 
GGAGAAGTAG CAACAATCAG TGCGGCAAAG ACATCCTGTC GTAAAATTGC CAGAGAAATG 
GGCTGGACAA AGGTAGCGGA CATTGAATCT GCAGGTCGGG CAGTATTTCA GAGGAAGGAA 
ATACGTGAAG AGTCCAACCC GAGAAAGCGA AAACTCTCCA CCCCACAAGT TGCGACTCGA 
CGGCAAGCGA GTAAAGCTAG TGATGATGTT GAGTCCGACG TGGAAACCGA AAATGAAGCT 
GACGCTCCTA GCCAAGGAGA AAAAAACGGC TTATCAAAGG AGAAGCCGTT GGCGAAACGT 
CGGAAGCGAA ACACCGTCGA ACCAGTCGTT AACACGTCAG TCGCGGTCGT CACTTGCGAA 
AGCACAGCCC GACGCCCTGG TATGCGGACC TCTCGAACCC CCGACCGTTT CGGTGTCGTT 
GCTGCGCCTC CGCAGAAGCG ACGCCGAAAG GTTTCACCCA AAGACGCCGA AGAGCCCAGT 
GAAGTTTTCG ATCTCGAATT CTCCGCTGCC ACCAATGTGC AAAGCTTGCT TTTGAAGCAA 
GTTCCCGGTA GGAGAAGACG TTCCAGGTCC CGAAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Phaeodactylum genome reveals the evolutionary history of diatom genomes.
Nature456(7219)239-44(2008 Nov)
Bowler C,Allen AE,Badger JH,Grimwood J,Jabbari K,Kuo A,Maheswari U,Martens C,Maumus F,Otillar RP,Rayko E,Salamov A,Vandepoele K,Beszteri B,Gruber A,Heijde M,Katinka M,Mock T,Valentin K,Verret F,Berges JA,Brownlee C,Cadoret JP,Chiovitti A,Choi CJ,Coesel S,De Martino A,Detter JC,Durkin C,Falciatore A,Fournet J,Haruta M,Huysman MJ,Jenkins BD,Jiroutova K,Jorgensen RE,Joubert Y,Kaplan A,Kröger N,Kroth PG,La Roche J,Lindquist E,Lommer M,Martin-Jézéquel V,Lopez PJ,Lucas S,Mangogna M,McGinnis K,Medlin LK,Montsant A,Oudot-Le Secq MP,Napoli C,Obornik M,Parker MS,Petit JL,Porcel BM,Poulsen N,Robison M,Rychlewski L,Rynearson TA,Schmutz J,Shapiro H,Siaut M,Stanley M,Sussman MR,Taylor AR,Vardi A,von Dassow P,Vyverman W,Willis A,Wyrwicz LS,Rokhsar DS,Weissenbach J,Armbrust EV,Green BR,Van de Peer Y,Grigoriev IV

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PHATRDRAFT_43159 cDNA ORF clone, Phaeodactylum tricornutum CCAP 1055/1

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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