LOC101122774 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101122774
Entrez Gene ID	101122774
Full Name	voltage-dependent N-type calcium channel subunit alpha-1B
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012161173.2	XP_012016563.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_012161174.2	XP_012016564.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_027966016.1	XP_027821817.1	voltage-dependent N-type calcium channel subunit alpha-1B
XM_015093793.1	XP_014949279.1	LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_012161172.2	XP_012016562.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101122774 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101122774 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv33135	XM_012161172.2 Latest version!	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOv33136	XM_012161173.2 Latest version!	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv33137	XM_012161174.2 Latest version!	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm76322	XM_027966016.1 Latest version!	Ovis aries calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101122774 ( XM_012161172.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012016562.1 Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv33135
Clone ID Related Accession (Same CDS sequence)	XM_012161172.2
Accession Version	XM_012161172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942949.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012161172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC 
CGCCTCTTCC GGGCCGCCCG GCTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATC 
CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ACGTGTGCCT GCTCATCGCC 
ATGCTGTTCT TCATCTATGC CATCATCGGC ATGCAGGTGT TCGGAAACAT CGCTCTGGAC 
GACGACACCA GCATCAACCG TCACAATAAC TTCCGCACGT TTCTTCAGGC CCTGATGTTG 
CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT 
CGGGCCTGTG ACGAGCTCGC CAACGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC 
TTCGTCTCCT TCATCTTCCT CTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC 
ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC 
GAATTCATCC GGGTCTGGGC TGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT 
GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG 
ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCACTGGA GATCAAGCTG 
GCTCCAGCTG GCATGAAGCA GCATCAGTGC GACGCAGAGC TGAGGAAGGA GATTTCCTCT 
GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCTGAC 
GAGATGACGG TGGGGAAGGT GTATGCAGCT CTGATGATAT TCGACTTCTA CAAGCAGAAC 
AAAACCACCA GAGACCAGAT GCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTA 
TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA 
GCCCGTGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA 
ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTA 
CCCTGCGAGG TGAGGACACC CCTGGAGCAT GGCCACTCCA CAGAGATCCC GGTGGTGCAG 
ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG CCCTGAGAGG CCCTGACGGG 
GAGCCCCAAC CCGGGCTGGA GAGCCAGGGC CGAGCGGCCT CCATGCCCCG CCTGGCAGCA 
GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC 
CAGAGGCCCC ACATGGCTCA TCTGTGCAAC GCGGCCCTGG ACCGTGCTCC AGCCAGCCAG 
GCCGCCCCCC ACCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG 
GAGAAGGGGC CCAGCCTGTC TGCAGACACA GATGGCGCAC CCTACAGCAG CGCGGGGCCG 
GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG 
CGGGGCCGGT CCCAGGAGCG AAGGCAGCCT TCCTCCTCTT CCTCCGAGAA GCAGCGCTTC 
TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCACAGC CCAAGCCCTC TCTCAGCAGC 
CATCCCACGT CGCCCACTGC CGGGCAGGAG CCAGGACCCC CGAGACAGGG TAGTGGCTCC 
GTGAACGGGA GCCCCCTGCT GTCAACATCT GGTGCTAGCA CCCCTGGCCG CGGCGGGCGG 
AGGCAGCTCC CCCAGACCCC GCTGACCCCC CGTCCCAGCA TCACCTATAA AACGGCTAAC 
TCCTCGCCTG TCCACTTTGC CGGGGCTCAG ACCAGCCTCC CTGCCTTTTC CCCCGGCCGG 
CTTAGCCGTG GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCC 
CTGGCCCCCA GCTCCCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGCGAG 
GCTGCAGCCC GCACCCAGCC TGAGGACACG CTCACCTTCG AGGAGGCTGT GGCCACCAAC 
TCAGGCCGCT CCTCCCGGAC TTCCTATGTG TCCTCCCTGA CCTCGCAGTC CCACCCCCTC 
CGCCGGGTGC CCAACGGCTA CCACTGCACT CTGGGGCTCA GCTCGGGCGG GGGCGGCCGG 
GGGCGGCACA GCTACCACCA CCCTGACCAA GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012016562.1
CDS	1..7005
Translation	MVRFGDELGGRYGGPGGGERARGGGAGVAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFALHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDADPVGDFPCGREAPARLCEGDTECREYWAGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFLPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAAKRSRNDLIHAEEGEGRSADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRLVKAQSFYWAVLCVVALNTLCVAIVHYQQPQRLTTALYFAEFVFLGLFLAEMALKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATSRHLRPDMKTHLDRPLVVEPGRDGARGPAGGKARPEGGEAGEAADPPRRHHRHRDRDRDKAAAPAGEPDRADAPKAEGGDAGAREERARPRRSRSKEAAGPREARSERGRGPGPDGGRRHHRRGSPEEAAEREPRRHRAHRHAPEPSREGAPGSKGERRARHRGGPRAGPREAESGEEPARRHRARHRAPPALEEAEKEAEAEDRDKEPRNHQPRESHCDLEASVVTGVGPVHALPSTCLEKVEEQPEDADNQRNVTRMGSQPSDLSTAVHIPVTLTGPPGETTAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVAMQYFEMVILVVIALSSIALAAEDPVQTDSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLRNVLNILIVYVLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDRVMSECSLEKNERACIDFAISARPLTRHMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSSRACDELANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNDDMTVHFTSTLMALIRTALEIKLAPAGMKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMHQAPGGLAQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAVQTQEGGIKESVSWGTQRTQEVPCEVRTPLEHGHSTEIPVVQTGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPPPDASPMKRSISTLAPQRPHMAHLCNAALDRAPASQAAPHHHHRCHRRRDRKQKSLEKGPSLSADTDGAPYSSAGPGPPPPPGDGAAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPQPKPSLSSHPTSPTAGQEPGPPRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPSSRIGSDPYLGQRLDSEAAARTQPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGGGRGRHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_012161172.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012161172.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC 
CGCCTCTTCC GGGCCGCCCG GCTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATC 
CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ACGTGTGCCT GCTCATCGCC 
ATGCTGTTCT TCATCTATGC CATCATCGGC ATGCAGGTGT TCGGAAACAT CGCTCTGGAC 
GACGACACCA GCATCAACCG TCACAATAAC TTCCGCACGT TTCTTCAGGC CCTGATGTTG 
CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT 
CGGGCCTGTG ACGAGCTCGC CAACGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC 
TTCGTCTCCT TCATCTTCCT CTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC 
ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC 
GAATTCATCC GGGTCTGGGC TGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT 
GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG 
ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCACTGGA GATCAAGCTG 
GCTCCAGCTG GCATGAAGCA GCATCAGTGC GACGCAGAGC TGAGGAAGGA GATTTCCTCT 
GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCTGAC 
GAGATGACGG TGGGGAAGGT GTATGCAGCT CTGATGATAT TCGACTTCTA CAAGCAGAAC 
AAAACCACCA GAGACCAGAT GCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTA 
TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA 
GCCCGTGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA 
ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTA 
CCCTGCGAGG TGAGGACACC CCTGGAGCAT GGCCACTCCA CAGAGATCCC GGTGGTGCAG 
ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG CCCTGAGAGG CCCTGACGGG 
GAGCCCCAAC CCGGGCTGGA GAGCCAGGGC CGAGCGGCCT CCATGCCCCG CCTGGCAGCA 
GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC 
CAGAGGCCCC ACATGGCTCA TCTGTGCAAC GCGGCCCTGG ACCGTGCTCC AGCCAGCCAG 
GCCGCCCCCC ACCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG 
GAGAAGGGGC CCAGCCTGTC TGCAGACACA GATGGCGCAC CCTACAGCAG CGCGGGGCCG 
GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG 
CGGGGCCGGT CCCAGGAGCG AAGGCAGCCT TCCTCCTCTT CCTCCGAGAA GCAGCGCTTC 
TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCACAGC CCAAGCCCTC TCTCAGCAGC 
CATCCCACGT CGCCCACTGC CGGGCAGGAG CCAGGACCCC CGAGACAGGG TAGTGGCTCC 
GTGAACGGGA GCCCCCTGCT GTCAACATCT GGTGCTAGCA CCCCTGGCCG CGGCGGGCGG 
AGGCAGCTCC CCCAGACCCC GCTGACCCCC CGTCCCAGCA TCACCTATAA AACGGCTAAC 
TCCTCGCCTG TCCACTTTGC CGGGGCTCAG ACCAGCCTCC CTGCCTTTTC CCCCGGCCGG 
CTTAGCCGTG GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCC 
CTGGCCCCCA GCTCCCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGCGAG 
GCTGCAGCCC GCACCCAGCC TGAGGACACG CTCACCTTCG AGGAGGCTGT GGCCACCAAC 
TCAGGCCGCT CCTCCCGGAC TTCCTATGTG TCCTCCCTGA CCTCGCAGTC CCACCCCCTC 
CGCCGGGTGC CCAACGGCTA CCACTGCACT CTGGGGCTCA GCTCGGGCGG GGGCGGCCGG 
GGGCGGCACA GCTACCACCA CCCTGACCAA GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101122774 ( XM_012161173.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012016563.1 Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X3, mRNA.

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CloneID	OOv33136
Clone ID Related Accession (Same CDS sequence)	XM_012161173.2
Accession Version	XM_012161173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942949.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012161173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGAAC AACTTCATCA ACCTCAGCTT CCTCCGCCTC 
TTCCGGGCCG CCCGGCTCAT CAAGCTGCTC CGCCAAGGCT ACACCATCCG CATCCTGCTC 
TGGACCTTCG TCCAGTCCTT CAAGGCCCTG CCCTACGTGT GCCTGCTCAT CGCCATGCTG 
TTCTTCATCT ATGCCATCAT CGGCATGCAG GTGTTCGGAA ACATCGCTCT GGACGACGAC 
ACCAGCATCA ACCGTCACAA TAACTTCCGC ACGTTTCTTC AGGCCCTGAT GTTGCTCTTC 
AGGAGTGCCA CCGGGGAGGC CTGGCATGAG ATCATGCTGT CCTGCCTCAG CAGTCGGGCC 
TGTGACGAGC TCGCCAACGC CACGGAGTGT GGGAGTGACT TCGCCTACTT CTACTTCGTC 
TCCTTCATCT TCCTCTGCTC CTTTCTGATG TTGAACCTCT TTGTGGCTGT GATCATGGAC 
AATTTTGAGT ACCTTACGCG GGACTCTTCC ATCCTAGGGC CTCACCACCT GGACGAATTC 
ATCCGGGTCT GGGCTGAGTA CGACCCGGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCGCCC CTGGGGCTGG GGAAGAAATG CCCTGCTCGT 
GTTGCGTACA AGCGCCTGGT TCGTATGAAC ATGCCCATCT CCAATGACGA CATGACCGTC 
CACTTCACAT CCACGCTGAT GGCCCTCATC CGGACTGCAC TGGAGATCAA GCTGGCTCCA 
GCTGGCATGA AGCAGCATCA GTGCGACGCA GAGCTGAGGA AGGAGATTTC CTCTGTGTGG 
GCCAATCTGC CCCAGAAGAC CCTGGACTTA CTGGTGCCAC CCCATAAACC TGACGAGATG 
ACGGTGGGGA AGGTGTATGC AGCTCTGATG ATATTCGACT TCTACAAGCA GAACAAAACC 
ACCAGAGACC AGATGCACCA AGCTCCTGGA GGCCTGGCCC AGATGGGCCC CGTATCCCTG 
TTCCACCCCC TGAAGGCCAC GCTGGAGCAG ACTCAGCCGG CTGTGCTCCG AGGAGCCCGT 
GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC CTCAGCAACG GTGGGGCAGT ACAAACCCAA 
GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG GGCACTCAGA GGACCCAGGA GGTACCCTGC 
GAGGTGAGGA CACCCCTGGA GCATGGCCAC TCCACAGAGA TCCCGGTGGT GCAGACAGGA 
AAACCGGCTG TGGACGTCCA GATGCAGAGC ATGGCCCTGA GAGGCCCTGA CGGGGAGCCC 
CAACCCGGGC TGGAGAGCCA GGGCCGAGCG GCCTCCATGC CCCGCCTGGC AGCAGAGACT 
CAGCCACCCC CAGATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC ACCCCAGAGG 
CCCCACATGG CTCATCTGTG CAACGCGGCC CTGGACCGTG CTCCAGCCAG CCAGGCCGCC 
CCCCACCACC ACCACCGCTG CCATCGCCGC AGGGACCGGA AGCAGAAGTC CCTGGAGAAG 
GGGCCCAGCC TGTCTGCAGA CACAGATGGC GCACCCTACA GCAGCGCGGG GCCGGGCCCC 
CCGCCGCCCC CAGGGGATGG GGCAGCAGGC TGCCGGCGGG AGCGCAGGCA GGAGCGGGGC 
CGGTCCCAGG AGCGAAGGCA GCCTTCCTCC TCTTCCTCCG AGAAGCAGCG CTTCTACTCC 
TGTGACCGCT TTGGGGGCCG TGAGCCCCCA CAGCCCAAGC CCTCTCTCAG CAGCCATCCC 
ACGTCGCCCA CTGCCGGGCA GGAGCCAGGA CCCCCGAGAC AGGGTAGTGG CTCCGTGAAC 
GGGAGCCCCC TGCTGTCAAC ATCTGGTGCT AGCACCCCTG GCCGCGGCGG GCGGAGGCAG 
CTCCCCCAGA CCCCGCTGAC CCCCCGTCCC AGCATCACCT ATAAAACGGC TAACTCCTCG 
CCTGTCCACT TTGCCGGGGC TCAGACCAGC CTCCCTGCCT TTTCCCCCGG CCGGCTTAGC 
CGTGGCCTTT CGGAACACAA CGCCCTGCTC CAGAGAGACC CCCTCAGCCA GCCCCTGGCC 
CCCAGCTCCC GGATTGGCTC CGACCCTTAC CTGGGGCAGC GTCTGGACAG CGAGGCTGCA 
GCCCGCACCC AGCCTGAGGA CACGCTCACC TTCGAGGAGG CTGTGGCCAC CAACTCAGGC 
CGCTCCTCCC GGACTTCCTA TGTGTCCTCC CTGACCTCGC AGTCCCACCC CCTCCGCCGG 
GTGCCCAACG GCTACCACTG CACTCTGGGG CTCAGCTCGG GCGGGGGCGG CCGGGGGCGG 
CACAGCTACC ACCACCCTGA CCAAGACCAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012016563.1
CDS	1..6999
Translation	MVRFGDELGGRYGGPGGGERARGGGAGVAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFALHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDADPVGDFPCGREAPARLCEGDTECREYWAGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFLPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAAKRSRNDLIHAEEGEGRSADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRLVKAQSFYWAVLCVVALNTLCVAIVHYQQPQRLTTALYFAEFVFLGLFLAEMALKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATSRHLRPDMKTHLDRPLVVEPGRDGARGPAGGKARPEGGEAGEAADPPRRHHRHRDRDRDKAAAPAGEPDRADAPKAEGGDAGAREERARPRRSRSKEAAGPREARSERGRGPGPDGGRRHHRRGSPEEAAEREPRRHRAHRHAPEPSREGAPGSKGERRARHRGGPRAGPREAESGEEPARRHRARHRAPPALEEAEKEAEAEDRDKEPRNHQPRESHCDLEASVVTGVGPVHALPSTCLEKVEEQPEDADNQRNVTRMGSQPSDLSTAVHIPVTLTGPPGETTAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVAMQYFEMVILVVIALSSIALAAEDPVQTDSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLRNVLNILIVYVLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDRVMSECSLEKNERACIDFAISARPLTRHMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSSRACDELANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNDDMTVHFTSTLMALIRTALEIKLAPAGMKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMHQAPGGLAQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAVQTQEGGIKESVSWGTQRTQEVPCEVRTPLEHGHSTEIPVVQTGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPPPDASPMKRSISTLAPQRPHMAHLCNAALDRAPASQAAPHHHHRCHRRRDRKQKSLEKGPSLSADTDGAPYSSAGPGPPPPPGDGAAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPQPKPSLSSHPTSPTAGQEPGPPRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPSSRIGSDPYLGQRLDSEAAARTQPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGGGRGRHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_012161173.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012161173.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGAAC AACTTCATCA ACCTCAGCTT CCTCCGCCTC 
TTCCGGGCCG CCCGGCTCAT CAAGCTGCTC CGCCAAGGCT ACACCATCCG CATCCTGCTC 
TGGACCTTCG TCCAGTCCTT CAAGGCCCTG CCCTACGTGT GCCTGCTCAT CGCCATGCTG 
TTCTTCATCT ATGCCATCAT CGGCATGCAG GTGTTCGGAA ACATCGCTCT GGACGACGAC 
ACCAGCATCA ACCGTCACAA TAACTTCCGC ACGTTTCTTC AGGCCCTGAT GTTGCTCTTC 
AGGAGTGCCA CCGGGGAGGC CTGGCATGAG ATCATGCTGT CCTGCCTCAG CAGTCGGGCC 
TGTGACGAGC TCGCCAACGC CACGGAGTGT GGGAGTGACT TCGCCTACTT CTACTTCGTC 
TCCTTCATCT TCCTCTGCTC CTTTCTGATG TTGAACCTCT TTGTGGCTGT GATCATGGAC 
AATTTTGAGT ACCTTACGCG GGACTCTTCC ATCCTAGGGC CTCACCACCT GGACGAATTC 
ATCCGGGTCT GGGCTGAGTA CGACCCGGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCGCCC CTGGGGCTGG GGAAGAAATG CCCTGCTCGT 
GTTGCGTACA AGCGCCTGGT TCGTATGAAC ATGCCCATCT CCAATGACGA CATGACCGTC 
CACTTCACAT CCACGCTGAT GGCCCTCATC CGGACTGCAC TGGAGATCAA GCTGGCTCCA 
GCTGGCATGA AGCAGCATCA GTGCGACGCA GAGCTGAGGA AGGAGATTTC CTCTGTGTGG 
GCCAATCTGC CCCAGAAGAC CCTGGACTTA CTGGTGCCAC CCCATAAACC TGACGAGATG 
ACGGTGGGGA AGGTGTATGC AGCTCTGATG ATATTCGACT TCTACAAGCA GAACAAAACC 
ACCAGAGACC AGATGCACCA AGCTCCTGGA GGCCTGGCCC AGATGGGCCC CGTATCCCTG 
TTCCACCCCC TGAAGGCCAC GCTGGAGCAG ACTCAGCCGG CTGTGCTCCG AGGAGCCCGT 
GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC CTCAGCAACG GTGGGGCAGT ACAAACCCAA 
GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG GGCACTCAGA GGACCCAGGA GGTACCCTGC 
GAGGTGAGGA CACCCCTGGA GCATGGCCAC TCCACAGAGA TCCCGGTGGT GCAGACAGGA 
AAACCGGCTG TGGACGTCCA GATGCAGAGC ATGGCCCTGA GAGGCCCTGA CGGGGAGCCC 
CAACCCGGGC TGGAGAGCCA GGGCCGAGCG GCCTCCATGC CCCGCCTGGC AGCAGAGACT 
CAGCCACCCC CAGATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC ACCCCAGAGG 
CCCCACATGG CTCATCTGTG CAACGCGGCC CTGGACCGTG CTCCAGCCAG CCAGGCCGCC 
CCCCACCACC ACCACCGCTG CCATCGCCGC AGGGACCGGA AGCAGAAGTC CCTGGAGAAG 
GGGCCCAGCC TGTCTGCAGA CACAGATGGC GCACCCTACA GCAGCGCGGG GCCGGGCCCC 
CCGCCGCCCC CAGGGGATGG GGCAGCAGGC TGCCGGCGGG AGCGCAGGCA GGAGCGGGGC 
CGGTCCCAGG AGCGAAGGCA GCCTTCCTCC TCTTCCTCCG AGAAGCAGCG CTTCTACTCC 
TGTGACCGCT TTGGGGGCCG TGAGCCCCCA CAGCCCAAGC CCTCTCTCAG CAGCCATCCC 
ACGTCGCCCA CTGCCGGGCA GGAGCCAGGA CCCCCGAGAC AGGGTAGTGG CTCCGTGAAC 
GGGAGCCCCC TGCTGTCAAC ATCTGGTGCT AGCACCCCTG GCCGCGGCGG GCGGAGGCAG 
CTCCCCCAGA CCCCGCTGAC CCCCCGTCCC AGCATCACCT ATAAAACGGC TAACTCCTCG 
CCTGTCCACT TTGCCGGGGC TCAGACCAGC CTCCCTGCCT TTTCCCCCGG CCGGCTTAGC 
CGTGGCCTTT CGGAACACAA CGCCCTGCTC CAGAGAGACC CCCTCAGCCA GCCCCTGGCC 
CCCAGCTCCC GGATTGGCTC CGACCCTTAC CTGGGGCAGC GTCTGGACAG CGAGGCTGCA 
GCCCGCACCC AGCCTGAGGA CACGCTCACC TTCGAGGAGG CTGTGGCCAC CAACTCAGGC 
CGCTCCTCCC GGACTTCCTA TGTGTCCTCC CTGACCTCGC AGTCCCACCC CCTCCGCCGG 
GTGCCCAACG GCTACCACTG CACTCTGGGG CTCAGCTCGG GCGGGGGCGG CCGGGGGCGG 
CACAGCTACC ACCACCCTGA CCAAGACCAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101122774 ( XM_012161174.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012016564.1 Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X4, mRNA.

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CloneID	OOv33137
Clone ID Related Accession (Same CDS sequence)	XM_012161174.2
Accession Version	XM_012161174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942949.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012161174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC 
CGCCTCTTCC GGGCCGCCCG GCTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATC 
CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ACGTGTGCCT GCTCATCGCC 
ATGCTGTTCT TCATCTATGC CATCATCGGC ATGCAGGTGT TCGGAAACAT CGCTCTGGAC 
GACGACACCA GCATCAACCG TCACAATAAC TTCCGCACGT TTCTTCAGGC CCTGATGTTG 
CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT 
CGGGCCTGTG ACGAGCTCGC CAACGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC 
TTCGTCTCCT TCATCTTCCT CTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC 
ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC 
GAATTCATCC GGGTCTGGGC TGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT 
GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG 
ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCACTGGA GATCAAGCTG 
GCTCCAGCTG GCATGAAGCA GCATCAGTGC GACGCAGAGC TGAGGAAGGA GATTTCCTCT 
GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCTGAC 
GAGATGACGG TGGGGAAGGT GTATGCAGCT CTGATGATAT TCGACTTCTA CAAGCAGAAC 
AAAACCACCA GAGACCAGAT GCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTA 
TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA 
GCCCGTGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA 
ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTA 
CCCTGCGAGG TGAGGACACC CCTGGAGCAT GGCCACTCCA CAGAGATCCC GGTGGTGCAG 
ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG CCCTGAGAGG CCCTGACGGG 
GAGCCCCAAC CCGGGCTGGA GAGCCAGGGC CGAGCGGCCT CCATGCCCCG CCTGGCAGCA 
GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC 
CAGAGGCCCC ACATGGCTCA TCTGTGCAAC GCGGCCCTGG ACCGTGCTCC AGCCAGCCAG 
GCCGCCCCCC ACCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG 
GAGAAGGGGC CCAGCCTGTC TGCAGACACA GATGGCGCAC CCTACAGCAG CGCGGGGCCG 
GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG 
CGGGGCCGGT CCCAGGAGCG AAGGCAGCCT TCCTCCTCTT CCTCCGAGAA GCAGCGCTTC 
TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCACAGC CCAAGCCCTC TCTCAGCAGC 
CATCCCACGT CGCCCACTGC CGGGCAGGAG CCAGGACCCC CGAGACAGGT ATCCTCCCTG 
CCTTTTCCCC CGGCCGGCTT AGCCGTGGCC TTTCGGAACA CAACGCCCTG CTCCAGAGAG 
ACCCCCTCAG CCAGCCCCTG GCCCCCAGCT CCCGGATTGG CTCCGACCCT TACCTGGGGC 
AGCGTCTGGA CAGCGAGGCT GCAGCCCGCA CCCAGCCTGA GGACACGCTC ACCTTCGAGG 
AGGCTGTGGC CACCAACTCA GGCCGCTCCT CCCGGACTTC CTATGTGTCC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012016564.1
CDS	131..6847
Translation	MVRFGDELGGRYGGPGGGERARGGGAGVAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFALHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDADPVGDFPCGREAPARLCEGDTECREYWAGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFLPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAAKRSRNDLIHAEEGEGRSADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRLVKAQSFYWAVLCVVALNTLCVAIVHYQQPQRLTTALYFAEFVFLGLFLAEMALKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATSRHLRPDMKTHLDRPLVVEPGRDGARGPAGGKARPEGGEAGEAADPPRRHHRHRDRDRDKAAAPAGEPDRADAPKAEGGDAGAREERARPRRSRSKEAAGPREARSERGRGPGPDGGRRHHRRGSPEEAAEREPRRHRAHRHAPEPSREGAPGSKGERRARHRGGPRAGPREAESGEEPARRHRARHRAPPALEEAEKEAEAEDRDKEPRNHQPRESHCDLEASVVTGVGPVHALPSTCLEKVEEQPEDADNQRNVTRMGSQPSDLSTAVHIPVTLTGPPGETTAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVAMQYFEMVILVVIALSSIALAAEDPVQTDSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLRNVLNILIVYVLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDRVMSECSLEKNERACIDFAISARPLTRHMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSSRACDELANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNDDMTVHFTSTLMALIRTALEIKLAPAGMKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMHQAPGGLAQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAVQTQEGGIKESVSWGTQRTQEVPCEVRTPLEHGHSTEIPVVQTGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPPPDASPMKRSISTLAPQRPHMAHLCNAALDRAPASQAAPHHHHRCHRRRDRKQKSLEKGPSLSADTDGAPYSSAGPGPPPPPGDGAAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPQPKPSLSSHPTSPTAGQEPGPPRQVSSLPFPPAGLAVAFRNTTPCSRETPSASPWPPAPGLAPTLTWGSVWTARLQPAPSLRTRSPSRRLWPPTQAAPPGLPMCPP

Target ORF information:

RefSeq Version	XM_012161174.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon voltage-dependent N-type calcium channel subunit alpha-1B (LOC101122774), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012161174.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC 
AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG 
CTCAAAGCTG TGTTCGACTG CGTGGTGAAC TCCCTGAGGA ACGTCCTCAA CATCCTGATC 
GTCTACGTGC TCTTCATGTT CATATTTGCT GTCATCGCGG TGCAGCTCTT CAAGGGGAAG 
TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG 
GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC 
TATGACAACG TGCTCTGGGC CCTGCTGACT CTGTTCACTG TGTCCACCGG CGAAGGATGG 
CCCATGGTGC TGAAGCACTC GGTGGACGCC ACCTACGAGG AGCAGGGCCC GAGCCCCGGC 
TTCCGCATGG AGCTGTCCAT CTTCTACGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT 
GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAGAGTCATG 
TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCGTGCATCG ACTTCGCCAT CAGTGCCAGA 
CCGCTGACGC GGCACATGCC TCAGAACAGG CAGTCGTTCC AGTACAAGAC GTGGACGTTC 
GTGGTCTCGC CGCCCTTCGA GTACTTCATC ATGGCCATGA TAGCCCTCAA CACGGTGGTG 
CTGATGATGA AGTTCTACGA CGCGCCCTAC GAGTACGAGC TGATGCTGAA GTGTCTGAAC 
ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGATCATCGC CTTTGGAGTG 
CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC 
CGCCTCTTCC GGGCCGCCCG GCTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATC 
CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ACGTGTGCCT GCTCATCGCC 
ATGCTGTTCT TCATCTATGC CATCATCGGC ATGCAGGTGT TCGGAAACAT CGCTCTGGAC 
GACGACACCA GCATCAACCG TCACAATAAC TTCCGCACGT TTCTTCAGGC CCTGATGTTG 
CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT 
CGGGCCTGTG ACGAGCTCGC CAACGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC 
TTCGTCTCCT TCATCTTCCT CTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC 
ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC 
GAATTCATCC GGGTCTGGGC TGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT 
GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG 
ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCACTGGA GATCAAGCTG 
GCTCCAGCTG GCATGAAGCA GCATCAGTGC GACGCAGAGC TGAGGAAGGA GATTTCCTCT 
GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCTGAC 
GAGATGACGG TGGGGAAGGT GTATGCAGCT CTGATGATAT TCGACTTCTA CAAGCAGAAC 
AAAACCACCA GAGACCAGAT GCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTA 
TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA 
GCCCGTGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA 
ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTA 
CCCTGCGAGG TGAGGACACC CCTGGAGCAT GGCCACTCCA CAGAGATCCC GGTGGTGCAG 
ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG CCCTGAGAGG CCCTGACGGG 
GAGCCCCAAC CCGGGCTGGA GAGCCAGGGC CGAGCGGCCT CCATGCCCCG CCTGGCAGCA 
GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC 
CAGAGGCCCC ACATGGCTCA TCTGTGCAAC GCGGCCCTGG ACCGTGCTCC AGCCAGCCAG 
GCCGCCCCCC ACCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG 
GAGAAGGGGC CCAGCCTGTC TGCAGACACA GATGGCGCAC CCTACAGCAG CGCGGGGCCG 
GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG 
CGGGGCCGGT CCCAGGAGCG AAGGCAGCCT TCCTCCTCTT CCTCCGAGAA GCAGCGCTTC 
TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCACAGC CCAAGCCCTC TCTCAGCAGC 
CATCCCACGT CGCCCACTGC CGGGCAGGAG CCAGGACCCC CGAGACAGGT ATCCTCCCTG 
CCTTTTCCCC CGGCCGGCTT AGCCGTGGCC TTTCGGAACA CAACGCCCTG CTCCAGAGAG 
ACCCCCTCAG CCAGCCCCTG GCCCCCAGCT CCCGGATTGG CTCCGACCCT TACCTGGGGC 
AGCGTCTGGA CAGCGAGGCT GCAGCCCGCA CCCAGCCTGA GGACACGCTC ACCTTCGAGG 
AGGCTGTGGC CACCAACTCA GGCCGCTCCT CCCGGACTTC CTATGTGTCC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101122774 ( XM_027966016.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027821817.1 Ovis aries calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

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CloneID	OSm76322
Clone ID Related Accession (Same CDS sequence)	XM_027966016.1
Accession Version	XM_027966016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	voltage-dependent N-type calcium channel subunit alpha-1B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC 
ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG 
CGGCTGCCCA AGCTCAAAGC TGTGTTCGAC TGCGTGGTGA ACTCCCTGAG GAACGTCCTC 
AACATCCTGA TCGTCTACGT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTGCAGCTC 
TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG 
GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCGCGGCA GTGGAAGAAA 
TACGACTTCC ACTATGACAA CGTGCTCTGG GCCCTGCTGA CTCTGTTCAC TGTGTCCACC 
GGCGAAGGAT GGCCCATGGT GCTGAAGCAC TCGGTGGACG CCACCTACGA GGAGCAGGGC 
CCGAGCCCCG GCTTCCGCAT GGAGCTGTCC ATCTTCTACG TGGTCTACTT CGTGGTCTTC 
CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG 
GACAGAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCGTGCAT CGACTTCGCC 
ATCAGTGCCA GACCGCTGAC GCGGCACATG CCTCAGAACA GGCAGTCGTT CCAGTACAAG 
ACGTGGACGT TCGTGGTCTC GCCGCCCTTC GAGTACTTCA TCATGGCCAT GATAGCCCTC 
AACACGGTGG TGCTGATGAT GAAGTTCTAC GACGCGCCCT ACGAGTACGA GCTGATGCTG 
AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGATCATC 
GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAC 
CTCAGCTTCC TCCGCCTCTT CCGGGCCGCC CGGCTCATCA AGCTGCTCCG CCAAGGCTAC 
ACCATCCGCA TCCTGCTCTG GACCTTCGTC CAGTCCTTCA AGGCCCTGCC CTACGTGTGC 
CTGCTCATCG CCATGCTGTT CTTCATCTAT GCCATCATCG GCATGCAGGT GTTCGGAAAC 
ATCGCTCTGG ACGACGACAC CAGCATCAAC CGTCACAATA ACTTTCGCAC GTTTCTTCAG 
GCCCTGATGT TGCTCTTCAG GAGTGCCACC GGGGAGGCCT GGCATGAGAT CATGCTGTCC 
TGCCTCAGCA GTCGGGCCTG TGACGAGCTC GCCAACGCCA CGGAGTGTGG GAGTGACTTC 
GCCTACTTCT ACTTCGTCTC CTTCATCTTC CTCTGCTCCT TTCTGATGTT GAACCTCTTT 
GTGGCTGTGA TCATGGACAA TTTTGAGTAC CTTACGCGGG ACTCTTCCAT CCTAGGGCCT 
CACCACCTGG ACGAATTCAT CCGGGTCTGG GCTGAGTACG ACCCGGCTGC GTGTGGGCGC 
ATCAGTTACA ATGACATGTT TGAGATGCTG AAACACATGT CCCCGCCCCT GGGGCTGGGG 
AAGAAATGCC CTGCTCGTGT TGCGTACAAG CGCCTGGTTC GTATGAACAT GCCCATCTCC 
AATGACGACA TGACCGTCCA CTTCACATCC ACGCTGATGG CCCTCATCCG GACTGCACTG 
GAGATCAAGC TGGCTCCAGC TGGCACGAAG CAGCATCAGT GCGACGCAGA GCTGAGGAAG 
GAGATTTCCT CTGTGTGGGC CAATCTGCCC CAGAAGACCC TGGACTTACT GGTGCCACCC 
CATAAACCTG ACGAGATGAC GGTGGGGAAG GTGTATGCAG CTCTGATGAT ATTCGACTTC 
TACAAGCAGA ACAAAACCAC CAGAGACCAG ATGCACCAAG CTCCTGGAGG CCTGGCCCAG 
ATGGGCCCCG TGTCCCTGTT CCACCCCCTG AAGGCCACGC TGGAGCAGAC TCAGCCGGCT 
GTGCTCCGAG GAGCCCGTGT TTTCCTTCGG CAGAAGAGCT CGGCCTCCCT CAGCAACGGT 
GGGGCAGTAC AAACCCAAGA GGGCGGCATC AAGGAGTCTG TCTCCTGGGG CACTCAGAGG 
ACCCAGGAGG TACCCTGCGA GGTGAGGACA CCCCTGGAGC ATGGCCACTC CACAGAGATC 
CCGGTGGTGC AGACAGGAAA ACCGGCTGTG GACGTCCAGA TGCAGAGCAT GGCCCTGAGA 
GGCCCTGACG GGGAGCCCCA ACCCGGGCTG GAGAGCCAGG GCCGAGCGGC CTCCATGCCC 
CGCCTGGCAG CAGAGACTCA GCCACCCCCA GATGCCAGCC CCATGAAGCG CTCCATCTCC 
ACACTGGCAC CCCAGAGGCC CCACATGGCT CATCTGTGCA ACGCGGCCCT GGACCGTGCT 
CCAGCCAGCC AGGCCGCCCC CCACCACCAC CACCGCTGCC ATCGCCGCAG GGACCGGAAG 
CAGAAGTCCC TGGAGAAGGG GCCCAGCCTG TCTGCAGACA CAGATGGCGC ACCCTACAGC 
AGCGCGGGGC CGGGCCCCCC GCCGCCCCCA GGGGATGGGG CAGCAGGCTG CCGGCGGGAG 
CGCAGGCAGG AGCGGGGCCG GTCCCAGGAG CGAAGGCAGC CTTCCTCCTC TTCCTCCGAG 
AAGCAGCGCT TCTACTCCTG TGACCGCTTT GGGGGCCGTG AGCCCCCACA GCCCAAGCCC 
TCTCTCAGCA GCCATCCCAC GTCGCCCACT GCCGGGCAGG AGCCAGGACC CCCGAGACAG 
GGTAGTGGCT CCGTGAACGG GAGCCCCCTG CTGTCAACAT CTGGTGCTAG CACCCCTGGC 
CGCGGCGGGC GGAGGCAGCT CCCCCAGACC CCGCTGACCC CCCGTCCCAG CATCACCTAT 
AAAACGGCTA ACTCCTCGCC TGTCCACTTT GCCGGGGCTC AGACCAGCCT CCCTGCCTTT 
TCCCCCGGCC GGCTTAGCCG TGGCCTTTCG GAACACAACG CCCTGCTCCA GAGAGACCCC 
CTCAGCCAGC CCCTGGCCCC CAGCTCCCGG ATTGGCTCCG ACCCTTACCT GGGGCAGCGT 
CTGGACAGCG AGGCTGCAGC CCGCACCCAG CCTGAGGACA CGCTCACCTT CGAGGAGGCT 
GTGGCCACCA ACTCAGGCCG CTCCTCCCGG ACTTCCTATG TGTCCTCCCT GACCTCGCAG 
TCCCACCCCC TCCGCCGGGT GCCCAACGGC TACCACTGCA CTCTGGGGCT CAGCTCGGGC 
GGGGGCGGCC GGGGGCGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027821817.1
CDS	1..7017
Translation	MVRFGDELGGRYGGPGGGERARGGGAGVAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFALHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDADPVGDFPCGREAPARLCEGDTECREYWAGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFLPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAAKRSRNDLIHAEEGEGRSADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRLVKAQSFYWAVLCVVALNTLCVAIVHYQQPQRLTTALYFAEFVFLGLFLAEMALKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATSRHLRPDMKTHLDRPLVVEPGRDGARGPAGGKARPEGGEAGEAADPPRRHHRHRDRDRDKAAAPAGEPDRADAPKAEGGDAGAREERARPRRSRSKEAAGPREARSERGRGPGPDGGRRHHRRGSPEEAAEREPRRHRAHRHAPEPSREGAPGSKGERRARHRGGPRAGPREAESGEEPARRHRARHRAPPALEEAEKEAEAEDRDKEPRNHQPRESHCDLEASVVTGVGPVHALPSTCLEKVEEQPEDADNQRNVTRMGSQPSDLSTAVHIPVTLTGPPGETTAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVAMQYFEMVILVVIALSSIALAAEDPVQTDSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLRNVLNILIVYVLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDRVMSECSLEKNERACIDFAISARPLTRHMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSSRACDELANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNDDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMHQAPGGLAQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAVQTQEGGIKESVSWGTQRTQEVPCEVRTPLEHGHSTEIPVVQTGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPPPDASPMKRSISTLAPQRPHMAHLCNAALDRAPASQAAPHHHHRCHRRRDRKQKSLEKGPSLSADTDGAPYSSAGPGPPPPPGDGAAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPQPKPSLSSHPTSPTAGQEPGPPRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPSSRIGSDPYLGQRLDSEAAARTQPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGGGRGRHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_027966016.1
Organism	Ovis aries(sheep)
Definition	Ovis aries calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966016.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGGGAGCGG 
GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC 
CAGCGGGTCC TCTACAAGCA GTCGATCGCA CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG 
ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCGC TGGAGCAGCA CCTCCCAGAC 
GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC 
TTCTGCTTCG AGGCGGGGAT CAAGATCGTC GCGCTGGGCT TTGCCTTACA CAAGGGCTCC 
TATCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC 
ACAGCTGGCA CTGACTTTGA CCTGCGAACG CTGCGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTCCCGCTCC TGCAGATCGG GCTGCTGCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT 
GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA 
GACCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC 
ACCGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC 
CTGTTCGCTA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC 
TACAACACCA ACGACGCGGC CGGCAACACC TGGAACTGGC TCTACTTTCT GCCCCTGATT 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC 
AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG 
ATCGAGCGGG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA AGTCATGCTG 
GCCGAGGAGG ACAAGAACGC GGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC 
GCCAAGAGGA GCCGAAACGA CCTGATCCAT GCGGAGGAGG GAGAGGGCCG CTCTGCAGAC 
CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGTCTCA AGAGCGGGAA GACGGAGAGC 
TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG 
GCCCAGAGCT TCTACTGGGC GGTGCTGTGC GTGGTGGCCC TGAACACGCT GTGCGTGGCC 
ATCGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCTC TGTACTTTGC AGAGTTTGTC 
TTCCTGGGCC TCTTCCTCGC AGAGATGGCC CTGAAGATGT ACGGCCTGGG GCCCAGGAGC 
TACTTCCGGT CGTCCTTCAA CTGCTTCGAT TTTGGGGTCA TCGTGGGGAG CATTTTCGAA 
GTGGTCTGGG CGGCCATCAA GCCAGGGACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG 
CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTCA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC 
GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT TCAGGATGAG 
ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACTGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AGTCACAAGG TGGAGTCAGC 
AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG 
CTGAATGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG 
GACGAAGAGG AGATGGAGGA AGCGGCCAAT CAGAAGCTTG CTCTGCAGAA GGCGAAAGAA 
GTGGCAGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG 
CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG 
CCAGGACGGG ACGGGGCGCG GGGGCCCGCG GGCGGCAAGG CCCGGCCCGA GGGCGGGGAG 
GCCGGCGAGG CTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACCGGGAC 
AAGGCCGCAG CCCCGGCGGG GGAGCCGGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG 
GACGCGGGCG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA 
GGCCCCCGCG AGGCCCGGAG CGAGCGGGGC CGCGGGCCGG GCCCGGACGG CGGCCGGCGC 
CACCACCGGC GGGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG 
CACCGGCACG CGCCGGAGCC GAGCCGGGAG GGCGCGCCCG GGAGCAAAGG CGAGCGGCGC 
GCGCGGCACC GCGGCGGCCC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCT 
GCCCGGCGGC ACCGAGCGCG ACACAGGGCG CCGCCCGCGC TCGAGGAGGC GGAGAAGGAG 
GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGGAACCACC AGCCCAGGGA GTCGCACTGT 
GACCTGGAGG CCAGTGTGGT GACTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACCTGT 
CTAGAGAAGG TGGAAGAACA GCCTGAGGAT GCAGACAATC AGCGGAACGT CACTCGCATG 
GGTAGTCAGC CCTCGGATCT GAGCACCGCT GTGCATATTC CAGTGACGCT GACCGGGCCT 
CCCGGCGAGA CCACGGCTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA 
AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGCGGCC CCCGACCCAT CGTCCCGTAC 
AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTTCGCC GCTTCTGCCA CTACATCGTG 
GCCATGCAGT ACTTTGAGAT GGTCATCCTC GTGGTCATCG CCCTGAGTAG CATTGCCCTG 
GCCGCTGAAG ACCCCGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC 
TACATCTTCA CAGGCGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA CTTGGGACTG 
CTGCTGCACC CTGGCGCCTA CTTCCGGGAT CTGTGGAACA TCCTGGACTT CATTGTGGTC 
AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC 
ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG 
CGGCTGCCCA AGCTCAAAGC TGTGTTCGAC TGCGTGGTGA ACTCCCTGAG GAACGTCCTC 
AACATCCTGA TCGTCTACGT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTGCAGCTC 
TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG 
GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCGCGGCA GTGGAAGAAA 
TACGACTTCC ACTATGACAA CGTGCTCTGG GCCCTGCTGA CTCTGTTCAC TGTGTCCACC 
GGCGAAGGAT GGCCCATGGT GCTGAAGCAC TCGGTGGACG CCACCTACGA GGAGCAGGGC 
CCGAGCCCCG GCTTCCGCAT GGAGCTGTCC ATCTTCTACG TGGTCTACTT CGTGGTCTTC 
CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG 
GACAGAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCGTGCAT CGACTTCGCC 
ATCAGTGCCA GACCGCTGAC GCGGCACATG CCTCAGAACA GGCAGTCGTT CCAGTACAAG 
ACGTGGACGT TCGTGGTCTC GCCGCCCTTC GAGTACTTCA TCATGGCCAT GATAGCCCTC 
AACACGGTGG TGCTGATGAT GAAGTTCTAC GACGCGCCCT ACGAGTACGA GCTGATGCTG 
AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGATCATC 
GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAC 
CTCAGCTTCC TCCGCCTCTT CCGGGCCGCC CGGCTCATCA AGCTGCTCCG CCAAGGCTAC 
ACCATCCGCA TCCTGCTCTG GACCTTCGTC CAGTCCTTCA AGGCCCTGCC CTACGTGTGC 
CTGCTCATCG CCATGCTGTT CTTCATCTAT GCCATCATCG GCATGCAGGT GTTCGGAAAC 
ATCGCTCTGG ACGACGACAC CAGCATCAAC CGTCACAATA ACTTTCGCAC GTTTCTTCAG 
GCCCTGATGT TGCTCTTCAG GAGTGCCACC GGGGAGGCCT GGCATGAGAT CATGCTGTCC 
TGCCTCAGCA GTCGGGCCTG TGACGAGCTC GCCAACGCCA CGGAGTGTGG GAGTGACTTC 
GCCTACTTCT ACTTCGTCTC CTTCATCTTC CTCTGCTCCT TTCTGATGTT GAACCTCTTT 
GTGGCTGTGA TCATGGACAA TTTTGAGTAC CTTACGCGGG ACTCTTCCAT CCTAGGGCCT 
CACCACCTGG ACGAATTCAT CCGGGTCTGG GCTGAGTACG ACCCGGCTGC GTGTGGGCGC 
ATCAGTTACA ATGACATGTT TGAGATGCTG AAACACATGT CCCCGCCCCT GGGGCTGGGG 
AAGAAATGCC CTGCTCGTGT TGCGTACAAG CGCCTGGTTC GTATGAACAT GCCCATCTCC 
AATGACGACA TGACCGTCCA CTTCACATCC ACGCTGATGG CCCTCATCCG GACTGCACTG 
GAGATCAAGC TGGCTCCAGC TGGCACGAAG CAGCATCAGT GCGACGCAGA GCTGAGGAAG 
GAGATTTCCT CTGTGTGGGC CAATCTGCCC CAGAAGACCC TGGACTTACT GGTGCCACCC 
CATAAACCTG ACGAGATGAC GGTGGGGAAG GTGTATGCAG CTCTGATGAT ATTCGACTTC 
TACAAGCAGA ACAAAACCAC CAGAGACCAG ATGCACCAAG CTCCTGGAGG CCTGGCCCAG 
ATGGGCCCCG TGTCCCTGTT CCACCCCCTG AAGGCCACGC TGGAGCAGAC TCAGCCGGCT 
GTGCTCCGAG GAGCCCGTGT TTTCCTTCGG CAGAAGAGCT CGGCCTCCCT CAGCAACGGT 
GGGGCAGTAC AAACCCAAGA GGGCGGCATC AAGGAGTCTG TCTCCTGGGG CACTCAGAGG 
ACCCAGGAGG TACCCTGCGA GGTGAGGACA CCCCTGGAGC ATGGCCACTC CACAGAGATC 
CCGGTGGTGC AGACAGGAAA ACCGGCTGTG GACGTCCAGA TGCAGAGCAT GGCCCTGAGA 
GGCCCTGACG GGGAGCCCCA ACCCGGGCTG GAGAGCCAGG GCCGAGCGGC CTCCATGCCC 
CGCCTGGCAG CAGAGACTCA GCCACCCCCA GATGCCAGCC CCATGAAGCG CTCCATCTCC 
ACACTGGCAC CCCAGAGGCC CCACATGGCT CATCTGTGCA ACGCGGCCCT GGACCGTGCT 
CCAGCCAGCC AGGCCGCCCC CCACCACCAC CACCGCTGCC ATCGCCGCAG GGACCGGAAG 
CAGAAGTCCC TGGAGAAGGG GCCCAGCCTG TCTGCAGACA CAGATGGCGC ACCCTACAGC 
AGCGCGGGGC CGGGCCCCCC GCCGCCCCCA GGGGATGGGG CAGCAGGCTG CCGGCGGGAG 
CGCAGGCAGG AGCGGGGCCG GTCCCAGGAG CGAAGGCAGC CTTCCTCCTC TTCCTCCGAG 
AAGCAGCGCT TCTACTCCTG TGACCGCTTT GGGGGCCGTG AGCCCCCACA GCCCAAGCCC 
TCTCTCAGCA GCCATCCCAC GTCGCCCACT GCCGGGCAGG AGCCAGGACC CCCGAGACAG 
GGTAGTGGCT CCGTGAACGG GAGCCCCCTG CTGTCAACAT CTGGTGCTAG CACCCCTGGC 
CGCGGCGGGC GGAGGCAGCT CCCCCAGACC CCGCTGACCC CCCGTCCCAG CATCACCTAT 
AAAACGGCTA ACTCCTCGCC TGTCCACTTT GCCGGGGCTC AGACCAGCCT CCCTGCCTTT 
TCCCCCGGCC GGCTTAGCCG TGGCCTTTCG GAACACAACG CCCTGCTCCA GAGAGACCCC 
CTCAGCCAGC CCCTGGCCCC CAGCTCCCGG ATTGGCTCCG ACCCTTACCT GGGGCAGCGT 
CTGGACAGCG AGGCTGCAGC CCGCACCCAG CCTGAGGACA CGCTCACCTT CGAGGAGGCT 
GTGGCCACCA ACTCAGGCCG CTCCTCCCGG ACTTCCTATG TGTCCTCCCT GACCTCGCAG 
TCCCACCCCC TCCGCCGGGT GCCCAACGGC TACCACTGCA CTCTGGGGCT CAGCTCGGGC 
GGGGGCGGCC GGGGGCGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101122774 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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