HCFC1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012164809.2	XP_012020199.1	host cell factor 1 isoform X2
XM_012164816.2	XP_012020206.1	host cell factor 1 isoform X9
XM_027963612.1	XP_027819413.1	host cell factor 1 isoform X3
XM_012164814.2	XP_012020204.1	host cell factor 1 isoform X7
XM_027963613.1	XP_027819414.1	host cell factor 1 isoform X4
XM_027963615.1	XP_027819416.1	host cell factor 1 isoform X6
XM_015104974.1	XP_014960460.1	host cell factor 1 isoform X1
XM_012164811.2	XP_012020201.1	host cell factor 1 isoform X4
XM_012164813.2	XP_012020203.1	host cell factor 1 isoform X6
XM_027963611.1	XP_027819412.1	host cell factor 1 isoform X2
XM_012164812.2	XP_012020202.1	host cell factor 1 isoform X5
XM_012164810.2	XP_012020200.1	host cell factor 1 isoform X3
XM_012164815.2	XP_012020205.1	host cell factor 1 isoform X8
XM_027963610.1	XP_027819411.1	host cell factor 1 isoform X1
XM_027963614.1	XP_027819415.1	host cell factor 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm56074	XM_015104974.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OOv35414	XM_012164816.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm85946	XM_027963615.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv35407	XM_012164809.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35408	XM_012164810.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35409	XM_012164811.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35410	XM_012164812.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35411	XM_012164813.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35412	XM_012164814.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv35413	XM_012164815.2 Latest version!	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm85941	XM_027963610.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm85942	XM_027963611.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm85943	XM_027963612.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm85944	XM_027963613.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm85945	XM_027963614.1 Latest version!	Ovis aries host cell factor C1 (HCFC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

HCFC1 ( XM_015104974.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014960460.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm56074
Clone ID Related Accession (Same CDS sequence)	XM_015104974.1
Accession Version	XM_015104974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639036.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CCACTGCCGT GCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014960460.1
CDS	430..1749
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAATAVQR

Target ORF information:

RefSeq Version	XM_015104974.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015104974.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CCACTGCCGT GCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164816.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020206.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OOv35414
Clone ID Related Accession (Same CDS sequence)	XM_012164816.2
Accession Version	XM_012164816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG 
CTCCAGGCCT CTCCAGGGCC GCGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC 
TCCGCGCCCC TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG TCCTGAGCTC 
CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC 
TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCAC CCACCCAGTC CGAAGTAGAC 
CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG 
GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC AGCCGCGCAG 
GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CTGTGCTCCA GGCTGCACAG 
CAGGCTGTCA TGGGCACTGG GGAGCCTATG GATACTTCAG AGGCGGCCGC GGCAGTGACG 
CAGGCAGAGC TGAGCCACCT GTCGGCTGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC 
ATTGTGCTAA CGCAACAGGA GCTGGCCGCC CTGGTCCAGC AGCAGCAGCT CCAGGAGGCG 
CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCTT TGGCCCCTGC CGACAGCCTC 
AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC 
GTGGGCCTAT TGCCCCCCAC GGCCACCGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC 
CCCCAGCCGG TCGTCGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACAGAA 
GTGGCCAACG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA 
GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TTATTAAGGG CACCAATGTG 
ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC 
ACCATGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG 
TTCCGCGTCG CCGGGATCAA TGCCTGTGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT 
AAAACATGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA 
GACGGTGCTC ACCTCACCTG GGAGCCGCCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC 
TCAGTTTACC TGGCCATCCA GAGCGCACAG GCCGGCGGCG AGACCAAGAG CTCGACCCCA 
GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC 
TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATC 
GCCGCCCGCA ACGAGAAGGG CTATGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC 
AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG 
AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020206.1
CDS	999..6977
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164816.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164816.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG 
CTCCAGGCCT CTCCAGGGCC GCGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC 
TCCGCGCCCC TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG TCCTGAGCTC 
CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC 
TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCAC CCACCCAGTC CGAAGTAGAC 
CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG 
GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC AGCCGCGCAG 
GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CTGTGCTCCA GGCTGCACAG 
CAGGCTGTCA TGGGCACTGG GGAGCCTATG GATACTTCAG AGGCGGCCGC GGCAGTGACG 
CAGGCAGAGC TGAGCCACCT GTCGGCTGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC 
ATTGTGCTAA CGCAACAGGA GCTGGCCGCC CTGGTCCAGC AGCAGCAGCT CCAGGAGGCG 
CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCTT TGGCCCCTGC CGACAGCCTC 
AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC 
GTGGGCCTAT TGCCCCCCAC GGCCACCGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC 
CCCCAGCCGG TCGTCGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACAGAA 
GTGGCCAACG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA 
GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TTATTAAGGG CACCAATGTG 
ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC 
ACCATGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG 
TTCCGCGTCG CCGGGATCAA TGCCTGTGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT 
AAAACATGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA 
GACGGTGCTC ACCTCACCTG GGAGCCGCCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC 
TCAGTTTACC TGGCCATCCA GAGCGCACAG GCCGGCGGCG AGACCAAGAG CTCGACCCCA 
GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC 
TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATC 
GCCGCCCGCA ACGAGAAGGG CTATGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC 
AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG 
AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963615.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819416.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X6, mRNA.

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CloneID	OSm85946
Clone ID Related Accession (Same CDS sequence)	XM_027963615.1
Accession Version	XM_027963615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG 
CTCCAGGCCT CTCCAGGGCC GCGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC 
TCCGCGCCCC TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG TCCTGAGCTC 
CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC 
TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCAC CCACCCAGTC CGAAGTAGAC 
CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG 
GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC AGCCGCGCAG 
GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CTGTGCTCCA GGCTGCACAG 
CAGGCTGTCA TGGGCACTGG GGAGCCTATG GATACTTCAG AGGCGGCCGC GGCAGTGACG 
CAGGCAGAGC TGAGCCACCT GTCGGCTGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC 
ATTGTGCTAA CGCAACAGGA GCTGGCCGCC CTGGTCCAGC AGCAGCAGCT CCAGGAGGCG 
CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCTT TGGCCCCTGC CGACAGCCTC 
AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC 
GTGGGCCTAT TGCCCCCCAC GGCCACCGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC 
CCCCAGCCGG TCGTCGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACAGAA 
GTGGCCAACG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA 
GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TTATTAAGGG CACCAATGTG 
ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC 
ACCATGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG 
TTCCGCGTCG CCGGGATCAA TGCCTGTGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT 
AAAACATGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA 
GACGGTGCTC ACCTCACCTG GGAGCCGCCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC 
TCAGTTTACC TGGCCATCCA GAGCGCACAG GCCGGCGGCG AGACCAAGAG CTCGACCCCA 
GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC 
TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATC 
GCCGCCCGCA ACGAGAAGGG CTATGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC 
AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG 
AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819416.1
CDS	1246..7224
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963615.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963615.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG 
CTCCAGGCCT CTCCAGGGCC GCGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC 
TCCGCGCCCC TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG TCCTGAGCTC 
CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC 
TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCAC CCACCCAGTC CGAAGTAGAC 
CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG 
GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC AGCCGCGCAG 
GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CTGTGCTCCA GGCTGCACAG 
CAGGCTGTCA TGGGCACTGG GGAGCCTATG GATACTTCAG AGGCGGCCGC GGCAGTGACG 
CAGGCAGAGC TGAGCCACCT GTCGGCTGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC 
ATTGTGCTAA CGCAACAGGA GCTGGCCGCC CTGGTCCAGC AGCAGCAGCT CCAGGAGGCG 
CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCTT TGGCCCCTGC CGACAGCCTC 
AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC 
GTGGGCCTAT TGCCCCCCAC GGCCACCGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC 
CCCCAGCCGG TCGTCGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACAGAA 
GTGGCCAACG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA 
GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TTATTAAGGG CACCAATGTG 
ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC 
ACCATGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG 
TTCCGCGTCG CCGGGATCAA TGCCTGTGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT 
AAAACATGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA 
GACGGTGCTC ACCTCACCTG GGAGCCGCCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC 
TCAGTTTACC TGGCCATCCA GAGCGCACAG GCCGGCGGCG AGACCAAGAG CTCGACCCCA 
GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC 
TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATC 
GCCGCCCGCA ACGAGAAGGG CTATGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC 
AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG 
AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164809.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020199.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X2, mRNA.

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CloneID	OOv35407
Clone ID Related Accession (Same CDS sequence)	XM_012164809.2
Accession Version	XM_012164809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA 
AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTTATTAAGG GCACCAATGT GATGGTGACA 
CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCATGCCC 
GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC 
GCCGGGATCA ATGCCTGTGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACATGT 
CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT 
CACCTCACCT GGGAGCCGCC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTTTAC 
CTGGCCATCC AGAGCGCACA GGCCGGCGGC GAGACCAAGA GCTCGACCCC AGCGCAGCTG 
GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC 
AACGAGAAGG GCTATGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC 
AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG 
CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020199.1
CDS	1001..7150
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164809.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164809.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA 
AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTTATTAAGG GCACCAATGT GATGGTGACA 
CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCATGCCC 
GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC 
GCCGGGATCA ATGCCTGTGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACATGT 
CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT 
CACCTCACCT GGGAGCCGCC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTTTAC 
CTGGCCATCC AGAGCGCACA GGCCGGCGGC GAGACCAAGA GCTCGACCCC AGCGCAGCTG 
GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC 
AACGAGAAGG GCTATGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC 
AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG 
CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164810.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020200.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X3, mRNA.

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CloneID	OOv35408
Clone ID Related Accession (Same CDS sequence)	XM_012164810.2
Accession Version	XM_012164810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020200.1
CDS	1000..7146
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164810.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164810.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164811.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020201.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X4, mRNA.

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CloneID	OOv35409
Clone ID Related Accession (Same CDS sequence)	XM_012164811.2
Accession Version	XM_012164811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG 
ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT 
GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA 
GGGCCGCGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCCTGGTG 
GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGTCCTG AGCTCCAGGC CGCCGTGGAT 
CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC 
ACCGTGGTGG TCCAGCCACC GCCACCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC 
CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC 
CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCAGCCG CGCAGGCTGC AGCCACAGAG 
GAGGCCCAGG CCCTGGCCAT CCAGGCTGTG CTCCAGGCTG CACAGCAGGC TGTCATGGCA 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020201.1
CDS	1002..7148
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164811.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164811.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG 
ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT 
GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA 
GGGCCGCGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCCTGGTG 
GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGTCCTG AGCTCCAGGC CGCCGTGGAT 
CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC 
ACCGTGGTGG TCCAGCCACC GCCACCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC 
CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC 
CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCAGCCG CGCAGGCTGC AGCCACAGAG 
GAGGCCCAGG CCCTGGCCAT CCAGGCTGTG CTCCAGGCTG CACAGCAGGC TGTCATGGCA 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164812.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020202.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X5, mRNA.

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CloneID	OOv35410
Clone ID Related Accession (Same CDS sequence)	XM_012164812.2
Accession Version	XM_012164812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020202.1
CDS	1002..7148
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164812.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164812.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164813.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020203.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X6, mRNA.

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CloneID	OOv35411
Clone ID Related Accession (Same CDS sequence)	XM_012164813.2
Accession Version	XM_012164813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT 
GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCGCGCCAGC AGCTTCCACC ACGGCAGCTC 
CTGCAGCCTG CCTCCGCGCC CCTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG 
AGTCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG 
GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC ACCCACCCAG 
TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC 
ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA 
GCAGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCTGTGCTC 
CAGGCTGCAC AGCAGGCTGT CATGGCAGGC ACTGGGGAGC CTATGGATAC TTCAGAGGCG 
GCCGCGGCAG TGACGCAGGC AGAGCTGAGC CACCTGTCGG CTGAGGGCCA GGAAGGCCAG 
GCGACCACTA TCCCCATTGT GCTAACGCAA CAGGAGCTGG CCGCCCTGGT CCAGCAGCAG 
CAGCTCCAGG AGGCGCAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCTTTGGCC 
CCTGCCGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG 
GCTGTGCCCA GCACCGTGGG CCTATTGCCC CCCACGGCCA CCGAGAGCCT GGCCCCGTCC 
AACACATTTG TGGCCCCCCA GCCGGTCGTC GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG 
GCCACCCTGA CAGAAGTGGC CAACGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA 
CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTTATT 
AAGGGCACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT 
GATGATGACT CGGGCACCAT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA 
GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GTGGCCGGGG GCCCTTCAGT 
GAGATCTCAG CCTTTAAAAC ATGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA 
ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CGCCCTCTGT GACCTCCGGC 
AAGATCATCG AGTACTCAGT TTACCTGGCC ATCCAGAGCG CACAGGCCGG CGGCGAGACC 
AAGAGCTCGA CCCCAGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC 
TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC 
ATCATCTTCC GCATCGCCGC CCGCAACGAG AAGGGCTATG GCCCAGCCAC CCAAGTCAGG 
TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG 
TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020203.1
CDS	1000..7113
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164813.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164813.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT 
GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCGCGCCAGC AGCTTCCACC ACGGCAGCTC 
CTGCAGCCTG CCTCCGCGCC CCTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG 
AGTCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG 
GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC ACCCACCCAG 
TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC 
ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA 
GCAGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCTGTGCTC 
CAGGCTGCAC AGCAGGCTGT CATGGCAGGC ACTGGGGAGC CTATGGATAC TTCAGAGGCG 
GCCGCGGCAG TGACGCAGGC AGAGCTGAGC CACCTGTCGG CTGAGGGCCA GGAAGGCCAG 
GCGACCACTA TCCCCATTGT GCTAACGCAA CAGGAGCTGG CCGCCCTGGT CCAGCAGCAG 
CAGCTCCAGG AGGCGCAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCTTTGGCC 
CCTGCCGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG 
GCTGTGCCCA GCACCGTGGG CCTATTGCCC CCCACGGCCA CCGAGAGCCT GGCCCCGTCC 
AACACATTTG TGGCCCCCCA GCCGGTCGTC GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG 
GCCACCCTGA CAGAAGTGGC CAACGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA 
CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTTATT 
AAGGGCACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT 
GATGATGACT CGGGCACCAT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA 
GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GTGGCCGGGG GCCCTTCAGT 
GAGATCTCAG CCTTTAAAAC ATGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA 
ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CGCCCTCTGT GACCTCCGGC 
AAGATCATCG AGTACTCAGT TTACCTGGCC ATCCAGAGCG CACAGGCCGG CGGCGAGACC 
AAGAGCTCGA CCCCAGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC 
TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC 
ATCATCTTCC GCATCGCCGC CCGCAACGAG AAGGGCTATG GCCCAGCCAC CCAAGTCAGG 
TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG 
TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164814.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020204.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X7, mRNA.

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CloneID	OOv35412
Clone ID Related Accession (Same CDS sequence)	XM_012164814.2
Accession Version	XM_012164814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCTATGG ATACTTCAGA GGCGGCCGCG GCAGTGACGC AGGCAGAGCT GAGCCACCTG 
TCGGCTGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TTGTGCTAAC GCAACAGGAG 
CTGGCCGCCC TGGTCCAGCA GCAGCAGCTC CAGGAGGCGC AGGCCCAGCA GCAGCAGCAC 
CACCTGCCCA CGGAAGCTTT GGCCCCTGCC GACAGCCTCA ATGACCCGAC CATCGAGAGC 
AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTATT GCCCCCCACG 
GCCACCGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTCGTGGCC 
AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACAGAAG TGGCCAACGG CATTGAGTCC 
CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC 
CAGTGGTTTG ACGTGGGGGT TATTAAGGGC ACCAATGTGA TGGTGACACA CTATTTCCTG 
CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCATGCCCGA CTACAACCAG 
CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT 
GCCTGTGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACATGTCT GCCTGGCTTC 
CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG 
GAGCCGCCCT CTGTGACCTC CGGCAAGATC ATCGAGTACT CAGTTTACCT GGCCATCCAG 
AGCGCACAGG CCGGCGGCGA GACCAAGAGC TCGACCCCAG CGCAGCTGGC CTTCATGCGG 
GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATCGACTACA CCACCAAGCC CGCCATCATC TTCCGCATCG CCGCCCGCAA CGAGAAGGGC 
TATGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG 
AAGGCAGACG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020204.1
CDS	1001..7018
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164814.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164814.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCTATGG ATACTTCAGA GGCGGCCGCG GCAGTGACGC AGGCAGAGCT GAGCCACCTG 
TCGGCTGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TTGTGCTAAC GCAACAGGAG 
CTGGCCGCCC TGGTCCAGCA GCAGCAGCTC CAGGAGGCGC AGGCCCAGCA GCAGCAGCAC 
CACCTGCCCA CGGAAGCTTT GGCCCCTGCC GACAGCCTCA ATGACCCGAC CATCGAGAGC 
AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTATT GCCCCCCACG 
GCCACCGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTCGTGGCC 
AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACAGAAG TGGCCAACGG CATTGAGTCC 
CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC 
CAGTGGTTTG ACGTGGGGGT TATTAAGGGC ACCAATGTGA TGGTGACACA CTATTTCCTG 
CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCATGCCCGA CTACAACCAG 
CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT 
GCCTGTGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACATGTCT GCCTGGCTTC 
CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG 
GAGCCGCCCT CTGTGACCTC CGGCAAGATC ATCGAGTACT CAGTTTACCT GGCCATCCAG 
AGCGCACAGG CCGGCGGCGA GACCAAGAGC TCGACCCCAG CGCAGCTGGC CTTCATGCGG 
GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATCGACTACA CCACCAAGCC CGCCATCATC TTCCGCATCG CCGCCCGCAA CGAGAAGGGC 
TATGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG 
AAGGCAGACG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_012164815.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012020205.1 Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X8, mRNA.

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CloneID	OOv35413
Clone ID Related Accession (Same CDS sequence)	XM_012164815.2
Accession Version	XM_012164815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	host cell factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGG CACTGGGGAG 
CCTATGGATA CTTCAGAGGC GGCCGCGGCA GTGACGCAGG CAGAGCTGAG CCACCTGTCG 
GCTGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATTG TGCTAACGCA ACAGGAGCTG 
GCCGCCCTGG TCCAGCAGCA GCAGCTCCAG GAGGCGCAGG CCCAGCAGCA GCAGCACCAC 
CTGCCCACGG AAGCTTTGGC CCCTGCCGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC 
TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGG GCCTATTGCC CCCCACGGCC 
ACCGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCCC AGCCGGTCGT CGTGGCCAGC 
CCCGCGAAGC TGCAGGCCGC GGCCACCCTG ACAGAAGTGG CCAACGGCAT TGAGTCCCTG 
GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG 
TGGTTTGACG TGGGGGTTAT TAAGGGCACC AATGTGATGG TGACACACTA TTTCCTGCCA 
CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCA TGCCCGACTA CAACCAGCTA 
AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC 
TGTGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CATGTCTGCC TGGCTTCCCG 
GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG 
CCGCCCTCTG TGACCTCCGG CAAGATCATC GAGTACTCAG TTTACCTGGC CATCCAGAGC 
GCACAGGCCG GCGGCGAGAC CAAGAGCTCG ACCCCAGCGC AGCTGGCCTT CATGCGGGTG 
TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATC 
GACTACACCA CCAAGCCCGC CATCATCTTC CGCATCGCCG CCCGCAACGA GAAGGGCTAT 
GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG 
CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG 
GCAGACGGTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012020205.1
CDS	1000..7014
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_012164815.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon host cell factor C1 (HCFC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164815.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGG CACTGGGGAG 
CCTATGGATA CTTCAGAGGC GGCCGCGGCA GTGACGCAGG CAGAGCTGAG CCACCTGTCG 
GCTGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATTG TGCTAACGCA ACAGGAGCTG 
GCCGCCCTGG TCCAGCAGCA GCAGCTCCAG GAGGCGCAGG CCCAGCAGCA GCAGCACCAC 
CTGCCCACGG AAGCTTTGGC CCCTGCCGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC 
TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGG GCCTATTGCC CCCCACGGCC 
ACCGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCCC AGCCGGTCGT CGTGGCCAGC 
CCCGCGAAGC TGCAGGCCGC GGCCACCCTG ACAGAAGTGG CCAACGGCAT TGAGTCCCTG 
GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG 
TGGTTTGACG TGGGGGTTAT TAAGGGCACC AATGTGATGG TGACACACTA TTTCCTGCCA 
CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCA TGCCCGACTA CAACCAGCTA 
AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC 
TGTGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CATGTCTGCC TGGCTTCCCG 
GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG 
CCGCCCTCTG TGACCTCCGG CAAGATCATC GAGTACTCAG TTTACCTGGC CATCCAGAGC 
GCACAGGCCG GCGGCGAGAC CAAGAGCTCG ACCCCAGCGC AGCTGGCCTT CATGCGGGTG 
TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATC 
GACTACACCA CCAAGCCCGC CATCATCTTC CGCATCGCCG CCCGCAACGA GAAGGGCTAT 
GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG 
CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG 
GCAGACGGTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963610.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819411.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.

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CloneID	OSm85941
Clone ID Related Accession (Same CDS sequence)	XM_027963610.1
Accession Version	XM_027963610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA 
AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTTATTAAGG GCACCAATGT GATGGTGACA 
CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCATGCCC 
GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC 
GCCGGGATCA ATGCCTGTGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACATGT 
CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT 
CACCTCACCT GGGAGCCGCC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTTTAC 
CTGGCCATCC AGAGCGCACA GGCCGGCGGC GAGACCAAGA GCTCGACCCC AGCGCAGCTG 
GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC 
AACGAGAAGG GCTATGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC 
AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG 
CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819411.1
CDS	1248..7397
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963610.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963610.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCTAT GGATACTTCA GAGGCGGCCG CGGCAGTGAC GCAGGCAGAG 
CTGAGCCACC TGTCGGCTGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATTGTGCTA 
ACGCAACAGG AGCTGGCCGC CCTGGTCCAG CAGCAGCAGC TCCAGGAGGC GCAGGCCCAG 
CAGCAGCAGC ACCACCTGCC CACGGAAGCT TTGGCCCCTG CCGACAGCCT CAATGACCCG 
ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTA 
TTGCCCCCCA CGGCCACCGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG 
GTCGTCGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACAGA AGTGGCCAAC 
GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA 
AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTTATTAAGG GCACCAATGT GATGGTGACA 
CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCATGCCC 
GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC 
GCCGGGATCA ATGCCTGTGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACATGT 
CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT 
CACCTCACCT GGGAGCCGCC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTTTAC 
CTGGCCATCC AGAGCGCACA GGCCGGCGGC GAGACCAAGA GCTCGACCCC AGCGCAGCTG 
GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC 
AACGAGAAGG GCTATGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC 
AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG 
CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963611.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819412.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X2, mRNA.

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CloneID	OSm85942
Clone ID Related Accession (Same CDS sequence)	XM_027963611.1
Accession Version	XM_027963611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819412.1
CDS	1248..7394
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963611.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963611.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT 
CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT 
CCAGGGCCGC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCCTG 
GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGTC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG 
GCCACCGTGG TGGTCCAGCC ACCGCCACCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC 
CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC 
ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CCGCGCAGGC TGCAGCCACA 
GAGGAGGCCC AGGCCCTGGC CATCCAGGCT GTGCTCCAGG CTGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963612.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819413.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X3, mRNA.

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CloneID	OSm85943
Clone ID Related Accession (Same CDS sequence)	XM_027963612.1
Accession Version	XM_027963612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG 
ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT 
GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA 
GGGCCGCGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCCTGGTG 
GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGTCCTG AGCTCCAGGC CGCCGTGGAT 
CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC 
ACCGTGGTGG TCCAGCCACC GCCACCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC 
CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC 
CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCAGCCG CGCAGGCTGC AGCCACAGAG 
GAGGCCCAGG CCCTGGCCAT CCAGGCTGTG CTCCAGGCTG CACAGCAGGC TGTCATGGCA 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819413.1
CDS	1248..7394
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963612.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963612.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG 
ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT 
GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA 
GGGCCGCGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCCTGGTG 
GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGTCCTG AGCTCCAGGC CGCCGTGGAT 
CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC 
ACCGTGGTGG TCCAGCCACC GCCACCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC 
CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC 
CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCAGCCG CGCAGGCTGC AGCCACAGAG 
GAGGCCCAGG CCCTGGCCAT CCAGGCTGTG CTCCAGGCTG CACAGCAGGC TGTCATGGCA 
GGCACTGGGG AGCCTATGGA TACTTCAGAG GCGGCCGCGG CAGTGACGCA GGCAGAGCTG 
AGCCACCTGT CGGCTGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT TGTGCTAACG 
CAACAGGAGC TGGCCGCCCT GGTCCAGCAG CAGCAGCTCC AGGAGGCGCA GGCCCAGCAG 
CAGCAGCACC ACCTGCCCAC GGAAGCTTTG GCCCCTGCCG ACAGCCTCAA TGACCCGACC 
ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTATTG 
CCCCCCACGG CCACCGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC 
GTCGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACAGAAGT GGCCAACGGC 
ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG 
AAGGAGAACC AGTGGTTTGA CGTGGGGGTT ATTAAGGGCA CCAATGTGAT GGTGACACAC 
TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CATGCCCGAC 
TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC 
GGGATCAATG CCTGTGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACATGTCTG 
CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC 
CTCACCTGGG AGCCGCCCTC TGTGACCTCC GGCAAGATCA TCGAGTACTC AGTTTACCTG 
GCCATCCAGA GCGCACAGGC CGGCGGCGAG ACCAAGAGCT CGACCCCAGC GCAGCTGGCC 
TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC 
AACGCCCACA TCGACTACAC CACCAAGCCC GCCATCATCT TCCGCATCGC CGCCCGCAAC 
GAGAAGGGCT ATGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC 
TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA 
AAGAAATCGA AGGCAGACGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963613.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819414.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X4, mRNA.

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CloneID	OSm85944
Clone ID Related Accession (Same CDS sequence)	XM_027963613.1
Accession Version	XM_027963613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT 
GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCGCGCCAGC AGCTTCCACC ACGGCAGCTC 
CTGCAGCCTG CCTCCGCGCC CCTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG 
AGTCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG 
GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC ACCCACCCAG 
TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC 
ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA 
GCAGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCTGTGCTC 
CAGGCTGCAC AGCAGGCTGT CATGGCAGGC ACTGGGGAGC CTATGGATAC TTCAGAGGCG 
GCCGCGGCAG TGACGCAGGC AGAGCTGAGC CACCTGTCGG CTGAGGGCCA GGAAGGCCAG 
GCGACCACTA TCCCCATTGT GCTAACGCAA CAGGAGCTGG CCGCCCTGGT CCAGCAGCAG 
CAGCTCCAGG AGGCGCAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCTTTGGCC 
CCTGCCGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG 
GCTGTGCCCA GCACCGTGGG CCTATTGCCC CCCACGGCCA CCGAGAGCCT GGCCCCGTCC 
AACACATTTG TGGCCCCCCA GCCGGTCGTC GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG 
GCCACCCTGA CAGAAGTGGC CAACGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA 
CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTTATT 
AAGGGCACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT 
GATGATGACT CGGGCACCAT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA 
GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GTGGCCGGGG GCCCTTCAGT 
GAGATCTCAG CCTTTAAAAC ATGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA 
ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CGCCCTCTGT GACCTCCGGC 
AAGATCATCG AGTACTCAGT TTACCTGGCC ATCCAGAGCG CACAGGCCGG CGGCGAGACC 
AAGAGCTCGA CCCCAGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC 
TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC 
ATCATCTTCC GCATCGCCGC CCGCAACGAG AAGGGCTATG GCCCAGCCAC CCAAGTCAGG 
TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG 
TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819414.1
CDS	1247..7360
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSVTETAPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963613.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963613.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTGTGAC TAGCAGTGCC GGCATCAAGT CGCCCATCAC CATTATCACC 
ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA 
ATCGCTACAG GCCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG TGCACCTGGC 
CAGCCAGGCA CCATCCTGCG CACCGTGCCT ATGGGGGGTG TCCGCCTGGT TACCCCCGTT 
ACTGTCTCTG CTGTCAAGCC GGCCGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC 
ACGACCCTGG GCACGGTGAC AGGCACCGTC TCTACCAGCC TGGCCGGAGC CGGGGGCCAT 
AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA 
AGTCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCGG CGCAGACGAC GCTAACGGCG 
GCCGCTGGGC TCACCACGCC CACCATCACC ATGCAGCCTG TCTCCCAGCC TACTCAGGTG 
ACTCTGATAA CGGCACCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC 
ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC 
CAGGGTGACG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGTGAGACC 
CATGAGACGG GCACCACCAA CACAGCCACC ACCACCGTCG TCGCTAATCT CGGGGGGCAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG 
ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT 
GAAACTCACG AGACTGGCAC CACCAGCACA GCCACCACCG CCATGTCTGG CATCGGAGGT 
GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG 
GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCCCAGGTTT CCATCAAGCC TGCGTGCCAA 
ACCCGTCAGA CCAGCGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC 
TCAGCCGGCC CGCTCCTCAG ACCAAGTGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT 
CTCTTACAGC TGGGGCCGCT GAGTGCCCAG GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT 
GCCGGCCTGG GCCCGCTGGT GTCCATGGGG CGCCAGGTGG AGGTGCATCA CACGCACACG 
ACCAACACAG CCACTGTGGC CCGCTCTGCC ATGGGTGCCG GGGAGCCCCA TGAGCTGCTG 
GGGGCCCCCA CACTTGTGTA CGAGAGTTCA GCCAGTGCCT CTGTGACTGC CGCGGCCTTG 
GAAGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG 
ACCCACGAGA CGGGCACCAC CCACACACCC ACCACAGCCA CATCCGGAGG GGCTGCAGGC 
CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT 
TCCACTGGCA CGACCATGTC TGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC 
CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC 
AGCGCTTCGT TGCTCACTCC TTTCCCAACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG 
ACGCACGAGA CAGGCACTAC GCACACAGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC 
CAAGACCCCC CACCGCCTGC TGGTGACCAG GGAGAGGTGG AGAGCACCCA GGGAGACAGT 
GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACACGGGCC 
GTGACCACCG TGACGCAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT 
GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCGCGCCAGC AGCTTCCACC ACGGCAGCTC 
CTGCAGCCTG CCTCCGCGCC CCTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG 
AGTCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG 
GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC ACCCACCCAG 
TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC 
ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA 
GCAGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCTGTGCTC 
CAGGCTGCAC AGCAGGCTGT CATGGCAGGC ACTGGGGAGC CTATGGATAC TTCAGAGGCG 
GCCGCGGCAG TGACGCAGGC AGAGCTGAGC CACCTGTCGG CTGAGGGCCA GGAAGGCCAG 
GCGACCACTA TCCCCATTGT GCTAACGCAA CAGGAGCTGG CCGCCCTGGT CCAGCAGCAG 
CAGCTCCAGG AGGCGCAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCTTTGGCC 
CCTGCCGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG 
GCTGTGCCCA GCACCGTGGG CCTATTGCCC CCCACGGCCA CCGAGAGCCT GGCCCCGTCC 
AACACATTTG TGGCCCCCCA GCCGGTCGTC GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG 
GCCACCCTGA CAGAAGTGGC CAACGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA 
CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTTATT 
AAGGGCACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT 
GATGATGACT CGGGCACCAT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA 
GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GTGGCCGGGG GCCCTTCAGT 
GAGATCTCAG CCTTTAAAAC ATGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA 
ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CGCCCTCTGT GACCTCCGGC 
AAGATCATCG AGTACTCAGT TTACCTGGCC ATCCAGAGCG CACAGGCCGG CGGCGAGACC 
AAGAGCTCGA CCCCAGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC 
TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC 
ATCATCTTCC GCATCGCCGC CCGCAACGAG AAGGGCTATG GCCCAGCCAC CCAAGTCAGG 
TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG 
TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_027963614.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027819415.1 Ovis aries host cell factor C1 (HCFC1), transcript variant X5, mRNA.

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CloneID	OSm85945
Clone ID Related Accession (Same CDS sequence)	XM_027963614.1
Accession Version	XM_027963614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	host cell factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCTATGG ATACTTCAGA GGCGGCCGCG GCAGTGACGC AGGCAGAGCT GAGCCACCTG 
TCGGCTGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TTGTGCTAAC GCAACAGGAG 
CTGGCCGCCC TGGTCCAGCA GCAGCAGCTC CAGGAGGCGC AGGCCCAGCA GCAGCAGCAC 
CACCTGCCCA CGGAAGCTTT GGCCCCTGCC GACAGCCTCA ATGACCCGAC CATCGAGAGC 
AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTATT GCCCCCCACG 
GCCACCGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTCGTGGCC 
AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACAGAAG TGGCCAACGG CATTGAGTCC 
CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC 
CAGTGGTTTG ACGTGGGGGT TATTAAGGGC ACCAATGTGA TGGTGACACA CTATTTCCTG 
CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCATGCCCGA CTACAACCAG 
CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT 
GCCTGTGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACATGTCT GCCTGGCTTC 
CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG 
GAGCCGCCCT CTGTGACCTC CGGCAAGATC ATCGAGTACT CAGTTTACCT GGCCATCCAG 
AGCGCACAGG CCGGCGGCGA GACCAAGAGC TCGACCCCAG CGCAGCTGGC CTTCATGCGG 
GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATCGACTACA CCACCAAGCC CGCCATCATC TTCCGCATCG CCGCCCGCAA CGAGAAGGGC 
TATGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG 
AAGGCAGACG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027819415.1
CDS	1248..7265
Translation	MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPAAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGRGLPRCAGEKAGVTSSAGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAAGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEATAALVTSTVGQPNGSVVRVCSNPPCETHETGTTSTATTAMSGIGGGPRRDIRLACAATTIPTVVRVSVTAGASEGAQVSIKPACQTRQTSATSTTMTVMATGAPCSAGPLLRPSVALEAAGRGATLLQLGPLSAQVRPGGEERSLAGLGPLVSMGRQVEVHHTHTTNTATVARSAMGAGEPHELLGAPTLVYESSASASVTAAALEALLCPSATVTQVCSNPPCETHETGTTHTPTTATSGGAAGQPEGGQQPPASRPCETHQTASTGTTMSVSLGALLPDAAPSHRTLESSLEVAVPPAVAPQASASLLTPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPPAGDQGEVESTQGDSVNIASSSPITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQASPGPRQQLPPRQLLQPASAPLVGDSAEVLSASQSPELQAAVDLSSTGDPSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELSHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQQHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVGLLPPTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPEDAVPTDDDSGTMPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSAQAGGETKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_027963614.1
Organism	Ovis aries(sheep)
Definition	Ovis aries host cell factor C1 (HCFC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027963614.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCAGTGC TCTTACAGCC TCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG GGCTGTGGCT 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC 
GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCGGCTG TGAGAGGGGA CATCCCTCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG 
GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG 
AAGAGACTGA AAGCAAAGAC ACCCAAAAAT GGGCCTCCTC CGTGTCCTCG GCTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA 
GAGTCTCACA CTGCAGTGGT CTACACCGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCCTGGATAT CGAGACTTTG 
ACGTGGAATA AGCCCAGTCT CAGTGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC 
ACTACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACCCTGGC TTGTCTCAAC 
CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCAGG 
GCCCGTGCCG GCCACTGTGC AGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT 
GACGGCTACC GAAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA 
ACGGAAAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG 
GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG 
CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT 
CAAGGTGTCC CGGCTGTGCT GAAAGTGACC GGTCCTCAGG CTACCACGGG AACCCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC 
GCAGGCGTGC GGATGGTTGT GCCTACACAG AGTGCCCAGG GGACGGTCAT TGGCAGCAGC 
CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT 
CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CTACCATCGT CAAAACCGTG 
GCCGTGACGC CAGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCACCAGTC 
ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACCTCC 
GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CTGTACACAA GTCCGGGACC 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGAGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG 
GCATCTACAG GCCCGGTGAC TCAGATTATC CAGACCAAAG GACCCCTGCC AGCCGGGACC 
ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG 
GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC 
ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG 
GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG TGTGACTAGC 
AGTGCCGGCA TCAAGTCGCC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT 
GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACAGGCCA CGGGCAGCAA 
GGAGTGACCC AGGTGGTGCT GAAGGGTGCA CCTGGCCAGC CAGGCACCAT CCTGCGCACC 
GTGCCTATGG GGGGTGTCCG CCTGGTTACC CCCGTTACTG TCTCTGCTGT CAAGCCGGCC 
GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC 
ACCGTCTCTA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG 
CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGTC AGGTGATCAA CCCCACTGCC 
ATCACTGTGT CGGCGGCGCA GACGACGCTA ACGGCGGCCG CTGGGCTCAC CACGCCCACC 
ATCACCATGC AGCCTGTCTC CCAGCCTACT CAGGTGACTC TGATAACGGC ACCCAGCGGG 
GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA 
CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGACGTGCA GCCCGGCACC 
GTGACCCTGG TGTGCTCCAA CCCGCCGTGT GAGACCCATG AGACGGGCAC CACCAACACA 
GCCACCACCA CCGTCGTCGC TAATCTCGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTC 
GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT 
GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CTCACGAGAC TGGCACCACC 
AGCACAGCCA CCACCGCCAT GTCTGGCATC GGAGGTGGGC CGCGGCGAGA CATCCGGCTC 
GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA 
GAGGGAGCCC AGGTTTCCAT CAAGCCTGCG TGCCAAACCC GTCAGACCAG CGCAACCAGC 
ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCAG CCGGCCCGCT CCTCAGACCA 
AGTGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TACAGCTGGG GCCGCTGAGT 
GCCCAGGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCC 
ATGGGGCGCC AGGTGGAGGT GCATCACACG CACACGACCA ACACAGCCAC TGTGGCCCGC 
TCTGCCATGG GTGCCGGGGA GCCCCATGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG 
AGTTCAGCCA GTGCCTCTGT GACTGCCGCG GCCTTGGAAG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC 
ACACCCACCA CAGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT 
CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCTGTC 
AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG 
GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCAGCG CTTCGTTGCT CACTCCTTTC 
CCAACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC 
ACAGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCTGGT 
GACCAGGGAG AGGTGGAGAG CACCCAGGGA GACAGTGTGA ATATCGCCAG TTCCAGTCCC 
ATCACGACAA CAGTGTCCTC CACGCTGACA CGGGCCGTGA CCACCGTGAC GCAGTCCACG 
CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCGCGC 
CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCCTGGT GGGGGACTCC 
GCCGAGGTCC TGTCAGCCTC CCAGAGTCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT 
ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG 
GTCCAGCCAC CGCCACCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG 
ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG 
GCCCTGGCCA TCCAGGCTGT GCTCCAGGCT GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCTATGG ATACTTCAGA GGCGGCCGCG GCAGTGACGC AGGCAGAGCT GAGCCACCTG 
TCGGCTGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TTGTGCTAAC GCAACAGGAG 
CTGGCCGCCC TGGTCCAGCA GCAGCAGCTC CAGGAGGCGC AGGCCCAGCA GCAGCAGCAC 
CACCTGCCCA CGGAAGCTTT GGCCCCTGCC GACAGCCTCA ATGACCCGAC CATCGAGAGC 
AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTATT GCCCCCCACG 
GCCACCGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTCGTGGCC 
AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACAGAAG TGGCCAACGG CATTGAGTCC 
CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC 
CAGTGGTTTG ACGTGGGGGT TATTAAGGGC ACCAATGTGA TGGTGACACA CTATTTCCTG 
CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCATGCCCGA CTACAACCAG 
CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT 
GCCTGTGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACATGTCT GCCTGGCTTC 
CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG 
GAGCCGCCCT CTGTGACCTC CGGCAAGATC ATCGAGTACT CAGTTTACCT GGCCATCCAG 
AGCGCACAGG CCGGCGGCGA GACCAAGAGC TCGACCCCAG CGCAGCTGGC CTTCATGCGG 
GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATCGACTACA CCACCAAGCC CGCCATCATC TTCCGCATCG CCGCCCGCAA CGAGAAGGGC 
TATGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG 
AAGGCAGACG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HCFC1
Entrez Gene ID	101121918
Full Name	host cell factor C1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HCFC1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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