PRPF8 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF8
Entrez Gene ID	101116489
Full Name	pre-mRNA processing factor 8
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004012545.3	XP_004012594.1	pre-mRNA-processing-splicing factor 8
XM_012151090.2	XP_012006480.1	pre-mRNA-processing-splicing factor 8
XM_004012545.4	XP_004012594.1	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems
Gene Ontology

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv26715	XM_012151090.2 Latest version!	Ovis aries musimon pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm09686	XM_004012545.3 Latest version!	Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm61069	XM_004012545.4 Latest version!	Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF8 ( XM_012151090.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012006480.1 Ovis aries musimon pre-mRNA processing factor 8 (PRPF8), mRNA.

Price & Availability↑

CloneID	OOv26715
Clone ID Related Accession (Same CDS sequence)	XM_012151090.2
Accession Version	XM_012151090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942649.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012151090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACACTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
CTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CACACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012006480.1
CDS	161..7168
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_012151090.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012151090.2

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACACTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
CTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CACACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_004012545.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004012594.1 Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.

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CloneID	OSm09686
Clone ID Related Accession (Same CDS sequence)	XM_004012545.3
Accession Version	XM_004012545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004012545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA AGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC TTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC TAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAT 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004012594.1
CDS	161..7168
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_004012545.3
Organism	Ovis aries(sheep)
Definition	Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004012545.3

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA AGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC TTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC TAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAT 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_004012545.4 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004012594.1 Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.

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CloneID	OSm61069
Clone ID Related Accession (Same CDS sequence)	XM_004012545.4
Accession Version	XM_004012545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004012545.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA AGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCG CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC TTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACTT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATATGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC TAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAT 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004012594.1
CDS	82..7089
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_004012545.4
Organism	Ovis aries(sheep)
Definition	Ovis aries pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004012545.4

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC ACTAGCCCCG 
CTGCCAGACT ACATGTCGGA AGAGAAGCTG CAGGAGAAAG CCCGAAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGT GCCCTCAAGT ACATGCCACA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATCCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTAT TGGACTGGTT CTATGACCAT CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA CGACATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAT 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTCGAGCTC 
CCAGAGTTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAAT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTACAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CCTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ATAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCGTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTATGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAACGC 
ATCCGCCGCG GGGCCACTGT AGACAAGACC GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCCGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA TGGTCCTTAC 
ATCACAGCAG AGGAAGCGGT GGCAGTGTAT ACCACAACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCGC CCATCCCCTT CCCCCCGCTC TCCTATAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACTTGGTGCC AGTGTATGAT GTGGAGCCGC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTATG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGTGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTCATC 
GTTGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATC 
GTACAATACT ATGGCCTGGT GATGGACTTG CTTGTGTTGG GATTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAAAT GCCAAATGAT TTCCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT ATTCTTCAGG 
TTCACAGCGG ATGAGGCTCG GGACCTTATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGCATG 
CGCCTAATGA AGCATGATGT CAACCTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCCGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGCCAAAT CCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAGTGGA ACACAGCTCT TATTGGTCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTAAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTCTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCG CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC CCGAATCAAC 
ACTCTCTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACTGACA TGATCCAGGC CCTGGGTGGC 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC TTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGGAGAT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACTGGTATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTTA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCTGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACTT TTGAAGGGAA CTTGACCACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC TCTGATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCGCAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGTGCCCTGC ATGTGAACAA CGACCGCGCG AAGGTGATCC TGAAGCCAGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACGGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCGCCATCA 
CAGCAGCGGC AGCAGATCGC TGAGATTGAG AAGCAGACGA AGGAACAGTC GCAGCTCACT 
GCAACCCAGA CTCGCACTGT CAACAAGCAT GGCGACGAAA TAATCACCTC CACCACTAGC 
AACTACGAGA CCCAGACTTT CTCTTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATATGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCATACT 
CAGCCCAACG AGTCTCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC TAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTATTGT CAGACCGTTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAT 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PRPF8 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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