TCF20 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCF20
Entrez Gene ID	101114510
Full Name	transcription factor 20
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012153484.1	XP_012008874.1	transcription factor 20 isoform X1
XM_012153485.2	XP_012008875.1	transcription factor 20 isoform X4
XM_015088670.1	XP_014944156.1	transcription factor 20 isoform X2
XM_004007026.3	XP_004007075.3	transcription factor 20 isoform X2
XM_004007026.2	XP_004007075.2	LOW QUALITY PROTEIN: transcription factor 20 isoform X1
XM_015088671.1	XP_014944157.1	transcription factor 20 isoform X2
XM_012153483.2	XP_012008873.1	transcription factor 20 isoform X3
XM_027968172.1	XP_027823973.1	transcription factor 20 isoform X1

Pathways from BioSystems
Gene Ontology

The following TCF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv28227	XM_012153483.2 Latest version!	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv28228	XM_012153484.1 Latest version!	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv28229	XM_012153485.2 Latest version!	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm35456	XM_004007026.2 Latest version!	Ovis aries transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm78788	XM_004007026.3 Latest version!	Ovis aries transcription factor 20 (TCF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv28226	XM_015088670.1 Latest version!	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv28226	XM_015088671.1 Latest version!	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm78787	XM_027968172.1 Latest version!	Ovis aries transcription factor 20 (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCF20 ( XM_012153483.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012008873.1 Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.

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CloneID	OOv28227
Clone ID Related Accession (Same CDS sequence)	XM_012153483.2
Accession Version	XM_012153483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	transcription factor 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012153483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA CGGTATCGCC TGGACAGGAG CGTGAGGGCG CGGGCAAGGA GGGCTGCTGG 
CCTGCTGCTG CGCTGCTGAG CAGTATGCAG TCCTTCCGGG AGCAAAGCAG TTACCACGGG 
AACCAGCAGA GCTACCCCCA GGAGGTGCAC GGCTCATCCC GGATAGAGGA GTTCAGCCCT 
CGTCAGGCCC AGATGTTCCA GAATTTTGGG GGTGCTGGTG GTGGCAGCGG CGGCAGCAGC 
AGCGGTGGTG GACGACGAGC AACGGCGGCG GCGGCGGCCG CGATGGCCAG CGAGACCTCT 
GGCCATCAGG GCTACCAGGG CTTCAGGAAG GAGGCTGGAG ACTTCTACTA CATGGCAGGC 
AACAAGGACC CCGTGTCTGC AGGAACCCCG CAGCCTCCTC CGCGAAGGCC TTCCGGGCCG 
GTGCAGAGCT ACGGACCCCC CCAGGGGAGC AGCTTTGGCA ATCAGTATGG CAGTGAGGGT 
CACGTGGGCC AGTTTCAAGC ACAGCACTCT GCCCTTGGCG GTGTGTCTCA TTACTCACAG 
GATTACACAG GGCCGTTCTC TCCGGGGAGC GCTCAGTACC AGCAGCAGCC TTCCAGCCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA GTGCAGCAGC TGCGACAGCA GCTCTACCAG 
TCCCATCAGC CCCTGCCGCA GGCCGCCAGC CAGCCGGCGT CCGGTGCCTC CCACCTGCAG 
CCCATGCAGC GGCCCTCAGC TCTGCCGGCC TCGGCTGCCG GCTACCAGCT GAGAGTGGGG 
CAGTTTGGCC AGCACTACCA GTCTTCTGCC ACTGCCGCTG CCTCCTCCTT CCCATCGCCG 
CAGCGTTTCA GCCAGTCCGG GCAGAGCTAT GATGGCAGTT ACAGTGTGAA CGCTGGATCA 
CAGTATGAAG GGCATAACGT GGGCTCCAAC GCACAGGCTT ACGGAACACA ATCCAATTAT 
AGCTATCAGC CTCAATCTAT GAAGAATTTC GAACAGGCCA AGATTCCACC AGGGACCCAG 
CAGGGTCAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CACCCCCCTC AGCACGTGAT GCAGTATTCC AACGCCGCCA CGAAGCTACC CCTGCAAAGT 
CAGGTCGGGC AGTACAGCCA GCCCGAGGTT CCCGTGAGGT CCCCCATGCA GTTTCACCAG 
AACTTCAGCC CCATCTCTAA CCCTTCCCCA GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT 
AGCTCTACCC CATCTCCTCT CACGCAGAGC GGGGAGAACC TCCAGTGTGG GCAAGGCAAT 
GTGCCAATGG GCTCCAGAAA CAGAATCCTG CAGTTAATGC CTCAGCTCAG CCCAACCCCG 
TCAATGATGC CCAGTCCCAA CTCTCACGCT GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG 
GTGCCTGAAA AGCGGCTGAC CGATCCCGGC TTGAGTAGTT TGAGTGCCCT GAGTACTCAG 
GTGGCCAACC TTCCTAATAC GGTTCAGCAC ATGCTACTTT CCGACGCCCT GACCCCTCAG 
AAGAAGACCT CCAAGAGGCC TTCCTCGTCA TCCAAGAAAA CAGATAGCTG CCCAAACTCG 
GAAGGCTCCT CACAGGCCGA GGAACAGCTG AAGTCCCCAA TGGCAGAGTC GCTGGATGGA 
GGCTGCTCCA GCAGTTCTGA GGATCAAGGC GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC 
ACAAGCTCGG ACACCACCTA CAAGGGCGGG GCCTCGGAAA AGGCAGGCTC CTCACCAGCA 
CAGGGCACTC AGAACGAAGC CCCCCGACTC AGCACCAGTC CCGGAGCCCG AGAAGAGACC 
GCCTCGCCGG GTGCCAAGGA CACACCGCTG TCATCTGAGG GCAACCCGAA AGCCAACGAG 
AAGGCAGTCG GGGTGATTGT CTCCCGGGAA GCCATGACAG GCCGGGTGGA AAAGCCTGGG 
GGGCAGGACA AGGGCTCCCA GGAGGAGGAT CCTGCAGCCA CACAGAGGCC GCCCAGCACC 
GGGGGGGCAA AGGAAGCCAG CCACGCATCA CTTCCACAGC CAGAGCCGCT GGGAGGAGGC 
AGTAAAGGAA ACAAGAGCGG AGACAGTAAC TCCAACCACA ACGGGGAAGG GAATGGCCAG 
ACCGGGCACC CCGCAGGGGG CTCTGGTTTC ACAGGCAGGA CTGAGCCCAG CAAATCTCCT 
GGAAGCCTGC GCTATAGTTA CAAAGACAGT TTTGGGTCAG CCGTACCGAG AAACATGAGT 
GGCTTTGCTC AGTATCCTAC AGGACAAGAT AAAGGGGACT TCACCAGCCA CGGGGAGCGA 
AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC CTGCAGGAGG TGCTTCAGGG TTACCACCAC 
CACCCCGACA GGAGGTACTC CCGGAGTTCT CAGGAGCACC AGGCTATGGC TGGCAGCCTC 
GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG AGTCAGACCA ATGAGTTGGC TAGCAGGGGC 
CTTCTGAACA AAAGCATTGG GTCCCTGTTA GAAAACCCCC ACTGGGGTCC ATGGGAAAGG 
AAGTCAAGTG GCACAGCTCC TGAGATGAAA CAGATCAATT TGGCTGACTA TCCAATTCCC 
AGAAAGTTTG AAATAGAGCC TCCGTCATCA GCCCATGAGC CCGGGGGTTC CCTCTCTGAG 
AGAAGATCAG TCATCTGTGA CATTTCTCCA CTAAGACAGA TTGTCAGAGA CCCAGGGGCT 
CACTCGCTAG GACACATGGG CGCCGACACC AGACTCGGGA GGAATGAGCG TCTCAATCTC 
AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT GGGCTGGTGT CCATGGAAAC AAAGCTGAAA 
TCCCAGAGTG GGCAGATAAA AGAGGAGGAC TTTGAACAGT CCAAGTCCCA AGCTAGTTTC 
AACAACAAGA AATCAGGAGA CCACTGCCAC CCTGCTAGCA TCAAGCACGA GTCCTACCGA 
GGCAACGCCA GCCCCGGAGC GGCGACCCAC GACTCCATCT CAGACTACGG CCCGCAGGAC 
AGCAGACCCA CACCAATGCG GCGAGTCCCT GGCAGAGTTG GCGGTCGGGA GGGCATGAGG 
GGTCGGTCCC CTTCTCAGTA TCATGACTTT TCAGAAAAGT TGAAGATGTC CCCTGGGAGG 
AGCAGAGGCC CAGGGGGCGA CCCTCATCAC ATGAACCCAC ACATGACCTT CTCCGAGAGG 
GCCAACAGGA GTTCTTTGCA CGCCCCCTTT TCTCCCAATT CAGAAAGCCT GGCCTCTGCT 
TATCACACAA ACACACGTGC TCATGCTTAT GGGGACCCCA GTGCAGGTTT GAATTCCCAG 
CTCCATTACA AGAGACAGAT GTACCAACAG CAGCAAGAGG AATATAAGGA CTGGGGCGGC 
AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT CAGCACAGGC AGGAGGGGCC CCGCAAGAGT 
CCACGGCAGC AGCAGTTTCT TGACCGAGTC CGGAGCCCCC TGAAGAATGA CAAAGATGGC 
ATGATGTACG GCCCACCGGT GGGGACGCAC CATGACCCCA GCGGCCAGGA CGGCGGGCGC 
TGCCTCATGT CCAGTGATGG TCTTTCTAAC AAAGGCATTG AACTGAAGCA CAGCTCCCAG 
AAGTTACAGC AAGAGTCTTG CTGGGATCTC TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC 
GGCCCCCCAG GAATGTCCAG TCAGAAAAGG TACGGACCAC CCCACGAGAC CGACGGACAT 
GGGCTCACAG AGTCTACGCA GTCATCCAAA CCGAGTAACG TTATGCTGAG ACTTCCAGGT 
CAAGAGGATC ATTCTTCTCA AAACCCCTTA ATCATGAGGA GGCGTGTCCG TTCTTTTATC 
TCTCCCATTC CCAGTAAGAG ACAGTCACAG GACGTGAAGA ACAGCAACTC TGAAGATAAA 
GGGCGCCTCC TTCACCCGTC AAAAGAAGGC ACTGACAAAG CGTACAATTC CTATGCCCAT 
CTTTCTCACA GTCAGGAGAT CAAGTCCATC CCTAAGAGGG AGTCCTCCAA GGACCTTCCG 
AGTCCAGATA GTAGGAACTG CCCTGCGGTT ACCCTTACAA GTCCTGCTAA GACCAAAATC 
CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA CTGGAAGCTA TCGTCCAGAA GATCACATCC 
CCAAACATTA GGAGGAGTGC GTCCTCGAAC AGCGCAGAGG CCGGGGTAGA CACGGTTACT 
CTTGACGACA TCCTGTCTTT GAAAAGCGGC CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG 
GACGCTGAGC TGGAGAAGAG AAAAGGTGAG GTGATAGCTG AGCTGGCCTG TCCCGTAAGC 
CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG 
GACGACAAGG TGAAGACGGA GACGCACCCT GACGCTGTCG CTGCTGGGAA GGAGCCCCCT 
GGTGCCATGG CATCCGCAAC CTCACAGAAG CCTGGGAGTA ACCAGGGGAG ACCAGACGGG 
CCCCTGGGCG GGACAGCACC TTTAATCTTT CCTGACTCGA AGAGCGCACC TCCAGTGGGC 
ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT GAAGAGAAAG AGAGCGATGC CGTGACGATT 
TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG GGTTACTTCC CCTCAGGAAA GAAGAAAGGG 
AGACCCATTG GTAGCGTGAA TAAGCAGAAG AAACAGCAGC AGCCCCCGCC GCCACCCCCG 
CAGCCCCCTC AGATACCAGA GGGCTCTGCT GATGGAGAGC CAAAGCCGAA AAAGCAGAGG 
CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC 
GCGCCCATCG TAGAGCCCCA GGAGCCGGAG ATCAAGCTGA AGTACGCCAC CCAGCCCCTG 
GACAAAACCG ACGCCAAGAA CAAGTCTTTT TTCCCTTATA TCCACGTAGT AAATAAGTGT 
GAACTTGGAG CCGTGTGTAC AATCATCAAC GCCGAGGAGG AGGAGCAGAC CAAATTGGTG 
AGGGGGAGGA AGGGCCAGCG GTCCCTGACG CCTCCGCCCA GCAGCACCGA GAGCAAGGTT 
CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC CCCGTGGTGA CAGAGTCTTC TGTGATGGGG 
CACCTGGTTT GCTGTCTGTG CGGCAAGTGG GCCAGTTACC GGAACATGGG CGACCTCTTT 
GGACCCTTTT ACCCCCAAGA TTATGCAGCC ACTCTCCCCA AGAATCCACC TCCCAAGAGG 
GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC CGGCACAAAA GCGCTTCGAA CGGCTCCAAG 
ACGGACACTG AGGAGGAGGA CGAGCAGCAG CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG 
CACCCCAGGT TTAAGCGGCG ACACCGCTCG GAAGACTGTG CCGGAGGCCC CCGGTCCCTG 
TCCAGGGGGC TCCCTTGTAA AAAAGCCACC ACTGAGGGCA GCAGCGAAAA GACTGTTTTG 
GACTCAAAGC CCTCCGTGCC CACCACTTCA GAAGGTGGCC CCGAGTTGGA GTTACAAATC 
CCTGAACTAC CTCTCGACAG CAATGAATTT TGGGTCCACG AGGGTTGTAT TCTCTGGGCC 
AATGGAATCT ACCTGGTCTG TGGCCGGCTC TATGGCCTGC AGGAAGCGCT GGAAATAGCC 
AGAGAGATGA AATGTTCCCA CTGCCAGGAG GCAGGTGCCA CCTTGGGCTG CTACAACAAA 
GGCTGCTCCT TCCGATACCA TTACCCGTGT GCCATTGACG CAGATTGTTT GCTCCACGAG 
GAGAACTTCT CGGTGAGGTG CCCCAAGCAC AAGCCTCCCC TCCCATGCCC TGTCCCCCCC 
TTGCAGAACA AGACAGCGAA AGGCAGCCTC AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012008873.1
CDS	165..6161
Translation	MKETVSPGQEREGAGKEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEDEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_012153483.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012153483.2

ORF Insert Sequence:

ATGAAAGAGA CGGTATCGCC TGGACAGGAG CGTGAGGGCG CGGGCAAGGA GGGCTGCTGG 
CCTGCTGCTG CGCTGCTGAG CAGTATGCAG TCCTTCCGGG AGCAAAGCAG TTACCACGGG 
AACCAGCAGA GCTACCCCCA GGAGGTGCAC GGCTCATCCC GGATAGAGGA GTTCAGCCCT 
CGTCAGGCCC AGATGTTCCA GAATTTTGGG GGTGCTGGTG GTGGCAGCGG CGGCAGCAGC 
AGCGGTGGTG GACGACGAGC AACGGCGGCG GCGGCGGCCG CGATGGCCAG CGAGACCTCT 
GGCCATCAGG GCTACCAGGG CTTCAGGAAG GAGGCTGGAG ACTTCTACTA CATGGCAGGC 
AACAAGGACC CCGTGTCTGC AGGAACCCCG CAGCCTCCTC CGCGAAGGCC TTCCGGGCCG 
GTGCAGAGCT ACGGACCCCC CCAGGGGAGC AGCTTTGGCA ATCAGTATGG CAGTGAGGGT 
CACGTGGGCC AGTTTCAAGC ACAGCACTCT GCCCTTGGCG GTGTGTCTCA TTACTCACAG 
GATTACACAG GGCCGTTCTC TCCGGGGAGC GCTCAGTACC AGCAGCAGCC TTCCAGCCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA GTGCAGCAGC TGCGACAGCA GCTCTACCAG 
TCCCATCAGC CCCTGCCGCA GGCCGCCAGC CAGCCGGCGT CCGGTGCCTC CCACCTGCAG 
CCCATGCAGC GGCCCTCAGC TCTGCCGGCC TCGGCTGCCG GCTACCAGCT GAGAGTGGGG 
CAGTTTGGCC AGCACTACCA GTCTTCTGCC ACTGCCGCTG CCTCCTCCTT CCCATCGCCG 
CAGCGTTTCA GCCAGTCCGG GCAGAGCTAT GATGGCAGTT ACAGTGTGAA CGCTGGATCA 
CAGTATGAAG GGCATAACGT GGGCTCCAAC GCACAGGCTT ACGGAACACA ATCCAATTAT 
AGCTATCAGC CTCAATCTAT GAAGAATTTC GAACAGGCCA AGATTCCACC AGGGACCCAG 
CAGGGTCAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CACCCCCCTC AGCACGTGAT GCAGTATTCC AACGCCGCCA CGAAGCTACC CCTGCAAAGT 
CAGGTCGGGC AGTACAGCCA GCCCGAGGTT CCCGTGAGGT CCCCCATGCA GTTTCACCAG 
AACTTCAGCC CCATCTCTAA CCCTTCCCCA GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT 
AGCTCTACCC CATCTCCTCT CACGCAGAGC GGGGAGAACC TCCAGTGTGG GCAAGGCAAT 
GTGCCAATGG GCTCCAGAAA CAGAATCCTG CAGTTAATGC CTCAGCTCAG CCCAACCCCG 
TCAATGATGC CCAGTCCCAA CTCTCACGCT GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG 
GTGCCTGAAA AGCGGCTGAC CGATCCCGGC TTGAGTAGTT TGAGTGCCCT GAGTACTCAG 
GTGGCCAACC TTCCTAATAC GGTTCAGCAC ATGCTACTTT CCGACGCCCT GACCCCTCAG 
AAGAAGACCT CCAAGAGGCC TTCCTCGTCA TCCAAGAAAA CAGATAGCTG CCCAAACTCG 
GAAGGCTCCT CACAGGCCGA GGAACAGCTG AAGTCCCCAA TGGCAGAGTC GCTGGATGGA 
GGCTGCTCCA GCAGTTCTGA GGATCAAGGC GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC 
ACAAGCTCGG ACACCACCTA CAAGGGCGGG GCCTCGGAAA AGGCAGGCTC CTCACCAGCA 
CAGGGCACTC AGAACGAAGC CCCCCGACTC AGCACCAGTC CCGGAGCCCG AGAAGAGACC 
GCCTCGCCGG GTGCCAAGGA CACACCGCTG TCATCTGAGG GCAACCCGAA AGCCAACGAG 
AAGGCAGTCG GGGTGATTGT CTCCCGGGAA GCCATGACAG GCCGGGTGGA AAAGCCTGGG 
GGGCAGGACA AGGGCTCCCA GGAGGAGGAT CCTGCAGCCA CACAGAGGCC GCCCAGCACC 
GGGGGGGCAA AGGAAGCCAG CCACGCATCA CTTCCACAGC CAGAGCCGCT GGGAGGAGGC 
AGTAAAGGAA ACAAGAGCGG AGACAGTAAC TCCAACCACA ACGGGGAAGG GAATGGCCAG 
ACCGGGCACC CCGCAGGGGG CTCTGGTTTC ACAGGCAGGA CTGAGCCCAG CAAATCTCCT 
GGAAGCCTGC GCTATAGTTA CAAAGACAGT TTTGGGTCAG CCGTACCGAG AAACATGAGT 
GGCTTTGCTC AGTATCCTAC AGGACAAGAT AAAGGGGACT TCACCAGCCA CGGGGAGCGA 
AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC CTGCAGGAGG TGCTTCAGGG TTACCACCAC 
CACCCCGACA GGAGGTACTC CCGGAGTTCT CAGGAGCACC AGGCTATGGC TGGCAGCCTC 
GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG AGTCAGACCA ATGAGTTGGC TAGCAGGGGC 
CTTCTGAACA AAAGCATTGG GTCCCTGTTA GAAAACCCCC ACTGGGGTCC ATGGGAAAGG 
AAGTCAAGTG GCACAGCTCC TGAGATGAAA CAGATCAATT TGGCTGACTA TCCAATTCCC 
AGAAAGTTTG AAATAGAGCC TCCGTCATCA GCCCATGAGC CCGGGGGTTC CCTCTCTGAG 
AGAAGATCAG TCATCTGTGA CATTTCTCCA CTAAGACAGA TTGTCAGAGA CCCAGGGGCT 
CACTCGCTAG GACACATGGG CGCCGACACC AGACTCGGGA GGAATGAGCG TCTCAATCTC 
AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT GGGCTGGTGT CCATGGAAAC AAAGCTGAAA 
TCCCAGAGTG GGCAGATAAA AGAGGAGGAC TTTGAACAGT CCAAGTCCCA AGCTAGTTTC 
AACAACAAGA AATCAGGAGA CCACTGCCAC CCTGCTAGCA TCAAGCACGA GTCCTACCGA 
GGCAACGCCA GCCCCGGAGC GGCGACCCAC GACTCCATCT CAGACTACGG CCCGCAGGAC 
AGCAGACCCA CACCAATGCG GCGAGTCCCT GGCAGAGTTG GCGGTCGGGA GGGCATGAGG 
GGTCGGTCCC CTTCTCAGTA TCATGACTTT TCAGAAAAGT TGAAGATGTC CCCTGGGAGG 
AGCAGAGGCC CAGGGGGCGA CCCTCATCAC ATGAACCCAC ACATGACCTT CTCCGAGAGG 
GCCAACAGGA GTTCTTTGCA CGCCCCCTTT TCTCCCAATT CAGAAAGCCT GGCCTCTGCT 
TATCACACAA ACACACGTGC TCATGCTTAT GGGGACCCCA GTGCAGGTTT GAATTCCCAG 
CTCCATTACA AGAGACAGAT GTACCAACAG CAGCAAGAGG AATATAAGGA CTGGGGCGGC 
AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT CAGCACAGGC AGGAGGGGCC CCGCAAGAGT 
CCACGGCAGC AGCAGTTTCT TGACCGAGTC CGGAGCCCCC TGAAGAATGA CAAAGATGGC 
ATGATGTACG GCCCACCGGT GGGGACGCAC CATGACCCCA GCGGCCAGGA CGGCGGGCGC 
TGCCTCATGT CCAGTGATGG TCTTTCTAAC AAAGGCATTG AACTGAAGCA CAGCTCCCAG 
AAGTTACAGC AAGAGTCTTG CTGGGATCTC TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC 
GGCCCCCCAG GAATGTCCAG TCAGAAAAGG TACGGACCAC CCCACGAGAC CGACGGACAT 
GGGCTCACAG AGTCTACGCA GTCATCCAAA CCGAGTAACG TTATGCTGAG ACTTCCAGGT 
CAAGAGGATC ATTCTTCTCA AAACCCCTTA ATCATGAGGA GGCGTGTCCG TTCTTTTATC 
TCTCCCATTC CCAGTAAGAG ACAGTCACAG GACGTGAAGA ACAGCAACTC TGAAGATAAA 
GGGCGCCTCC TTCACCCGTC AAAAGAAGGC ACTGACAAAG CGTACAATTC CTATGCCCAT 
CTTTCTCACA GTCAGGAGAT CAAGTCCATC CCTAAGAGGG AGTCCTCCAA GGACCTTCCG 
AGTCCAGATA GTAGGAACTG CCCTGCGGTT ACCCTTACAA GTCCTGCTAA GACCAAAATC 
CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA CTGGAAGCTA TCGTCCAGAA GATCACATCC 
CCAAACATTA GGAGGAGTGC GTCCTCGAAC AGCGCAGAGG CCGGGGTAGA CACGGTTACT 
CTTGACGACA TCCTGTCTTT GAAAAGCGGC CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG 
GACGCTGAGC TGGAGAAGAG AAAAGGTGAG GTGATAGCTG AGCTGGCCTG TCCCGTAAGC 
CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG 
GACGACAAGG TGAAGACGGA GACGCACCCT GACGCTGTCG CTGCTGGGAA GGAGCCCCCT 
GGTGCCATGG CATCCGCAAC CTCACAGAAG CCTGGGAGTA ACCAGGGGAG ACCAGACGGG 
CCCCTGGGCG GGACAGCACC TTTAATCTTT CCTGACTCGA AGAGCGCACC TCCAGTGGGC 
ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT GAAGAGAAAG AGAGCGATGC CGTGACGATT 
TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG GGTTACTTCC CCTCAGGAAA GAAGAAAGGG 
AGACCCATTG GTAGCGTGAA TAAGCAGAAG AAACAGCAGC AGCCCCCGCC GCCACCCCCG 
CAGCCCCCTC AGATACCAGA GGGCTCTGCT GATGGAGAGC CAAAGCCGAA AAAGCAGAGG 
CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC 
GCGCCCATCG TAGAGCCCCA GGAGCCGGAG ATCAAGCTGA AGTACGCCAC CCAGCCCCTG 
GACAAAACCG ACGCCAAGAA CAAGTCTTTT TTCCCTTATA TCCACGTAGT AAATAAGTGT 
GAACTTGGAG CCGTGTGTAC AATCATCAAC GCCGAGGAGG AGGAGCAGAC CAAATTGGTG 
AGGGGGAGGA AGGGCCAGCG GTCCCTGACG CCTCCGCCCA GCAGCACCGA GAGCAAGGTT 
CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC CCCGTGGTGA CAGAGTCTTC TGTGATGGGG 
CACCTGGTTT GCTGTCTGTG CGGCAAGTGG GCCAGTTACC GGAACATGGG CGACCTCTTT 
GGACCCTTTT ACCCCCAAGA TTATGCAGCC ACTCTCCCCA AGAATCCACC TCCCAAGAGG 
GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC CGGCACAAAA GCGCTTCGAA CGGCTCCAAG 
ACGGACACTG AGGAGGAGGA CGAGCAGCAG CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG 
CACCCCAGGT TTAAGCGGCG ACACCGCTCG GAAGACTGTG CCGGAGGCCC CCGGTCCCTG 
TCCAGGGGGC TCCCTTGTAA AAAAGCCACC ACTGAGGGCA GCAGCGAAAA GACTGTTTTG 
GACTCAAAGC CCTCCGTGCC CACCACTTCA GAAGGTGGCC CCGAGTTGGA GTTACAAATC 
CCTGAACTAC CTCTCGACAG CAATGAATTT TGGGTCCACG AGGGTTGTAT TCTCTGGGCC 
AATGGAATCT ACCTGGTCTG TGGCCGGCTC TATGGCCTGC AGGAAGCGCT GGAAATAGCC 
AGAGAGATGA AATGTTCCCA CTGCCAGGAG GCAGGTGCCA CCTTGGGCTG CTACAACAAA 
GGCTGCTCCT TCCGATACCA TTACCCGTGT GCCATTGACG CAGATTGTTT GCTCCACGAG 
GAGAACTTCT CGGTGAGGTG CCCCAAGCAC AAGCCTCCCC TCCCATGCCC TGTCCCCCCC 
TTGCAGAACA AGACAGCGAA AGGCAGCCTC AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_012153484.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012008874.1 Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

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CloneID	OOv28228
Clone ID Related Accession (Same CDS sequence)	XM_012153484.1
Accession Version	XM_012153484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012008874.1
CDS	159..6125
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEDEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_012153484.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012153484.1

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_012153485.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012008875.1 Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.

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CloneID	OOv28229
Clone ID Related Accession (Same CDS sequence)	XM_012153485.2
Accession Version	XM_012153485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	transcription factor 20 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012153485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012008875.1
CDS	173..6085
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEDEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_012153485.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012153485.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_004007026.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004007075.2 Ovis aries transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

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CloneID	OSm35456
Clone ID Related Accession (Same CDS sequence)	XM_004007026.2
Accession Version	XM_004007026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 3, 2015 this sequence version replaced XM_004007026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CAGCAGGGNG GGGTGGGGGA CGACGAGCAA CGGCGGCGGC GGCGGCCGCG 
ATGGCCAGCG AGACCTCTGG CCATCAGGGC TACCAGGGCT TCAGGAAGGA GGCTGGAGAC 
TTCTACTACA TGGCAGGCAA CAAGGACCCC GTGTCTGCAG GAACCCCGCA GCCTCCTCCG 
CGAAGGCCTT CCGGGCCGGT GCAGAGCTAC GGACCCCCCC AGGGGAGCAG CTTTGGCAAT 
CAGTATGGCA GTGAGGGTCA CGTGGGCCAG TTTCAAGCAC AGCACTCTGC CCTTGGCGGT 
GTGTCTCATT ACTCACAGGA TTACACAGGG CCGTTCTCTC CGGGGAGCGC TCAGTACCAG 
CAGCAGCCTA CCCAGCGGCC CCAGCAGCAG CAGCAGCAGC AGCAAGTGCA GCAGCTGCGA 
CAGCAGCTCT ACCAGTCCCA TCAGCCCCTG CCGCAGGCCG CCAGCCAGCC GGCGTCCGGT 
GCCTCCCACC TGCAGCCCAT GCAGCGGCCC TCAGCTCTGN NGGCTGCCGG CTCCCAGCTG 
AGAGTGGGGC AGTTCGGCCA GCACTACCAG TCTTCTGCCA CTGCCGCTGC CTCCTCCTTC 
CCATCGCCGC AGCGTTTCAG CCAGTCCGGG CAGAGCTACG ATGGCAGTTA CAGTGTGAAC 
GCTGGATCAC AGTATGAAGG GCATAACGTG GGCTCCAACG CACAGGCTTA CGGAACACAA 
TCCAATTATA GCTATCAGCC TCAATCTATG AAGAATTTCG AACAGGCCAA GATTCCACCA 
GGGACCCAGC AGGGTCAGCA GCAGCAGGGC GGCGGCCGGC AGCAGCGGCA GCAGCACCCC 
CCTCGGCACG TGATGCAGTA TTCCAACGCC GCCACGAAGC TACCCCTGCA AAGTCAGGTC 
GGGCAGTACA GCCAGCCCGA GGTTCCCGTG AGGTCCCCCA TGCAGTTTCA CCAGAACTTC 
AGCCCCATCT CTAACCCTTC CCCAGCTGCC TCTGTGGTTC AGTCTCCAAG CTGTAGCTCT 
ACCCCATCTC CTCTCACGCA GAGCGGGGAG AACCTCCAGT GTGGGCAAGG CAATGTGCCA 
ATGGGCTCCA GAAACAGAAT CCTGCAGTTA ATGCCTCAGC TCAGCCCAAC CCCGTCAATG 
ATGCCCAGTC CCAACTCTCA CGCTGCGGGC TTCAAAGGGT TCGGGCTGGA AGGGGTGCCT 
GAAAAGCGGC TGACCGATCC CGGCTTGAGT AGTTTGAGTG CCCTGAGTAC TCAGGTGGCC 
AACCTTCCTA ATACGGTTCA GCACATGCTA CTTTCCGACG CCCTGACCCC TCAGAAGAAG 
ACCTCCAAGA GGCCTTCCTC GTCATCCAAG AAAACAGATA GCTGCCCAAA CTCGGAAGGC 
TCCTCACAGG CCGAGGAACA GCTGAAGTCC CCAATGGCAG AGTCGCTGGA TGGAGGCTGC 
TCCAGCAGTT CTGAGGATCA AGGCGAGAGG GTGAGGCAGC TGAGCGGCCA GAGCACAAGC 
TCGGACACCA CCTACAAGGG CGGGGCCTCG GAAAAGGCAG GCTCCTCACC AGCACAGGGC 
ACTCAGAACG AAGCCCCCCG ACTCAGCACC AGTCCCGGAG CCCGAGAAGA GACCGCCTCG 
CCGGGTGCCA AGGACACACC GCTGTCATCT GAGGGCAACC CGAAAGCCAA CGAGAAGGCA 
GTCGGGGTGA TTGTCTCCCG GGAAGCCATG ACAGGCCGGG TGGAAAAGCC TGGGGGGCAG 
GACAAGGGCT CCCAGGAGGA GGATCCTGCA GCCACACAGA GGCCGCCCAG CACCGGGGGG 
GCAAAGGAAG CCAGCCACGC ATCACTTCCA CAGCCAGAGC CGCTGGGAGG AGGCAGTAAA 
GGAAACAAGA GCGGAGACAG TAACTCCAAC CACAACGGGG AAGGGAATGG CCAGACCGGG 
CACCCCGCAG GGGGCTCTGG TTTCACAGGC AGGACTGAGC CCAGCAAATC TCCTGGAAGC 
CTGCGCTATA GTTACAAAGA CAGTTTTGGG TCAGCCGTAC CGAGAAACAT GAGTGGCTTT 
GCTCAGTATC CTACAGGACA AGATAAAGGG GACTTCACCA GCCACGGGGA GCGAAAGGGT 
AGAAATGAGA AGTTCCCCAG CCTCCTGCAG GAGGTGCTTC AGGGTTACCA CCACCACCCC 
GACAGGAGGT ACTCCCGGAG TTCTCAGGAG CACCAGGCTA TGGCTGGCAG CCTCGAAGGA 
GCCACAAGGC CTAACGTCTT AGTGAGTCAG ACCAATGAGT TGGCTAGCAG GGGCCTTCTG 
AACAAAAGCA TTGGGTCCCT GTTAGAAAAC CCCCACTGGG GTCCATGGGA AAGGAAGTCA 
AGTGGCACAG CTCCTGAGAT GAAACAGATC AATTTGGCTG ACTATCCAAT TCCCAGAAAG 
TTTGAAATAG AGCCTCCGTC ATCAGCCCAT GAGCCCGGGG GTTCCCTCTC TGAGAGAAGA 
TCAGTCATCT GTGACATTTC TCCACTAAGA CAGATTGTCA GAGACCCAGG GGCTCACTCG 
CTAGGACACA TGGGCGCCGA CACCAGACTC GGGAGGAATG AGCGTCTCAA TCTCAGTTTA 
AGTCAGTCAG TCATTCTTCC AGGTGGGCTG GTGTCCATGG AAACAAAGCT GAAATCCCAG 
AGTGGGCAGA TAAAAGAGGA GGACTTTGAA CAGTCCAAGT CCCAAGCTAG TTTCAACAAC 
AAGAAATCAG GAGACCACTG CCACCCTGCT AGCATCAAGC ACGAGTCCTA CCGAGGCAAC 
GCCAGCCCCG GAGCGGCGAC CCACGACTCC ATCTCAGACT ACGGCCCGCA GGACAGCAGA 
CCCACACCAA TGCGGCGAGT CCCTGGCAGA GTTGGCGGTC GGGAGGGCAT GAGGGGTCGG 
TCCCCTTCTC AGTATCATGA CTTTTCAGAA AAGTTGAAGA TGTCCCCTGG GAGGAGCAGA 
GGCCCAGGGG GCGACCCTCA TCACATGAAC CCACACATGA CCTTCTCCGA GAGGGCCAAC 
AGGAGTTCTT TGCACGCCCC CTTTTCTCCC AATTCAGAAA GCCTGGCCTC TGCTTATCAC 
ACAAACACAC GTGCTCATGC TTATGGGGAC CCCAGTGCAG GTTTGAATTC CCAGCTCCAT 
TACAAGAGAC AGATGTACCA ACAGCAGCAA GAGGAATATA AGGACTGGGG CGGCAGTTCT 
GCTCAGGGAG TGATCGCTGC GGCTCAGCAC AGGCAGGAGG GGCCCCGCAA GAGTCCACGG 
CAGCAGCAGT TTCTTGACCG AGTCCGGAGC CCCCTGAAGA ATGACAAAGA TGGCATGATG 
TACGGCCCAC CGGTGGGGAC GCACCATGAC CCCAGCGGCC AGGACGGCGG GCGCTGCCTC 
ATGTCCAGTG ATGGTCTTTC TAACAAAGGC ATTGAACTGA AGCACAGCTC CCAGAAGTTA 
CAGCAAGAGT CTTGCTGGGA TCTCTCTCGG CAGACTTCTC CAGCCAAAAG CAGCGGCCCC 
CCAGGAATGT CCAGTCAGAA AAGGTACGGA CCACCCCACG AGACCGACGG ACATGGGCTC 
ACAGAGTCTA CGCAGTCATC CAAACCGAGT AACGTTATGC TGAGACTTCC AGGTCAAGAG 
GATCATTCTT CTCAAAACCC CTTAATCATG AGGAGGCGTG TCCGTTCTTT TATCTCTCCC 
ATTCCCAGTA AGAGACAGTC ACAGGACGTG AAGAACAGCA ACTCTGAAGA TAAAGGGCGC 
CTCCTTCACC CGTCAAAAGA AGGCACTGAC AAAGCGTACA ATTCCTATGC CCATCTTTCT 
CACAGTCAGG AGATCAAGTC CATCCCTAAG AGGGAGTCCT CCAAGGACCT TCCGAGTCCA 
GATAGTAGGA ACTGCCCTGC GGTTACCCTT ACAAGTCCTG CTAAGACCAA AATCCTGCCC 
CCGAGGAAAG GCCGGGGGTT GAAACTGGAA GCTATCGTCC AGAAGATCAC ATCCCCAAAC 
ATTAGGAGGA GTGCGTCCTC GAACAGCGCA GAGGCCGGGG TAGACACGGT TACTCTTGAC 
GACATCCTGT CTTTGAAAAG CGGCCCTCCA GAAGGTGGGA GCGGTGCTGT TCCGGACGCT 
GAGCTGGAGA AGAGAAAAGG TGAGGTGATA GCTGAGCTGG CCTGTCCCGT AAGCCAGGAG 
CTGAGCGGAG AAAAGCCTCT GGCCCGGTCT TCGGAGGAGT GGCGCGGTGG TGGGGACGAC 
AAGGTGAAGA CGGAGACGCA CCCTGACGCT GTCGCTGCTG GGAAGGAGCC CCCTGGTGCC 
ATGGCATCCG CAACCTCACA GAAGCCTGGG AGTAACCAGG GGAGACCAGA CGGGCCCCTG 
GGCGGGACAG CACCTTTAAT CTTTCCTGAC TCGAAGAGCG CACCTCCAGT GGGCACGCTG 
GCTCCCGAGG CAAACCCCAA GGCTGAAGAG AAAGAGAGCG ATGCCGTGAC GATTTCCCCC 
AAACAGGAGG GCTTCCCCCC GAAGGGTTAC TTCCCCTCAG GAAAGAAGAA AGGGAGACCC 
ATTGGTAGCG TGAATAAGCA GAAGAAACAG CAGCAGCCTC CGCCGTCACC CCCGCAGCCC 
CCTNCCGCAC CCCGGGGCTC TGCTGATGGA GAGCCAAAGC CGAAAAAGCA GAGGCAGCGG 
AGGGAGCGCA GGAAGCCCGG GGCGCAGCCC AGGAAGCGGA GGGCCAAGCA GGCCGCGCCC 
ATCGTAGAGC CCCAGGAGCC GGAGATCAAG CTGAAGTACG CCACCCAGCC CCTGGACAAA 
ACCGACGCCA AGAACAAGTC TTTTTTCCCT TATATCCACG TAGTAAATAA GTGTGAACTT 
GGAGCCGTGT GTACAATCAT CAACGCCGAG GAGGAGGAGC AGACCAAATT GGTGAGGGGG 
AGGAAGGGCC AGCGGTCCCT GACGCCTCCG CCCAGCAGCA CCGAGAGCAA GGTTCTCCCA 
GCCTCGTCCT TCGTGCTGCA GGGCCCCGTG GTGACAGAGT CTTCTGTGAT GGGGCACCTG 
GTTTGCTGTC TGTGCGGCAA GTGGGCCAGT TACCGGAACA TGGGCGACCT CTTTGGACCC 
TTTTACCCCC AAGATTATGC AGCCACTCTC CCCAAGAATC CACCTCCCAA GAGGGCCGCG 
GAAACGCAGA GCAAGGTGAA GGTCCGGCAC AAAAGCGCTT CGAACGGCTC CAAGACGGAC 
ACTGAGGAGG AGGAAGAGCA GCAGCAGCAG AAGGAGCAGA GGAGCCTGGC TGCGCACCCC 
AGGTTTAAGC GGCGACACCG CTCGGAAGAC TGTGCCGGAG GCCCCCGGTC CCTGTCCAGG 
GGGCTCCCTT GTAAAAAAGC CACCACTGAG GGCAGCAGCG AAAAGACTGT TTTGGACTCA 
AAGCCCTCCG TGCCCACCAC TTCAGAAGGT GGCCCCGAGT TGGAGTTACA AATCCCTGAA 
CTACCTCTCG ACAGCAATGA ATTTTGGGTC CACGAGGGTT GTATTCTCTG GGCCAATGGA 
ATCTACCTGG TCTGTGGCCG GCTCTATGGC CTGCAGGAAG CGCTGGAAAT AGCCAGAGAG 
ATGAAATGTT CCCACTGCCA GGAGGCAGGT GCCACCTTGG GCTGCTACAA CAAAGGCTGC 
TCCTTCCGAT ACCATTACCC GTGTGCCATT GACGCAGATT GTTTGCTCCA CGAGGAGAAC 
TTCTCGGTGA GGTGCCCCAA GCACAAGGTG AGACTGTGGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004007075.2
CDS	156..6080
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAAGnullGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPTQRPQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALnullAAGSQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQGGGRQQRQQHPPRHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPSPPQPPnullAPRGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_004007026.2
Organism	Ovis aries(sheep)
Definition	Ovis aries transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004007026.2

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CAGCAGGGNG GGGTGGGGGA CGACGAGCAA CGGCGGCGGC GGCGGCCGCG 
ATGGCCAGCG AGACCTCTGG CCATCAGGGC TACCAGGGCT TCAGGAAGGA GGCTGGAGAC 
TTCTACTACA TGGCAGGCAA CAAGGACCCC GTGTCTGCAG GAACCCCGCA GCCTCCTCCG 
CGAAGGCCTT CCGGGCCGGT GCAGAGCTAC GGACCCCCCC AGGGGAGCAG CTTTGGCAAT 
CAGTATGGCA GTGAGGGTCA CGTGGGCCAG TTTCAAGCAC AGCACTCTGC CCTTGGCGGT 
GTGTCTCATT ACTCACAGGA TTACACAGGG CCGTTCTCTC CGGGGAGCGC TCAGTACCAG 
CAGCAGCCTA CCCAGCGGCC CCAGCAGCAG CAGCAGCAGC AGCAAGTGCA GCAGCTGCGA 
CAGCAGCTCT ACCAGTCCCA TCAGCCCCTG CCGCAGGCCG CCAGCCAGCC GGCGTCCGGT 
GCCTCCCACC TGCAGCCCAT GCAGCGGCCC TCAGCTCTGN NGGCTGCCGG CTCCCAGCTG 
AGAGTGGGGC AGTTCGGCCA GCACTACCAG TCTTCTGCCA CTGCCGCTGC CTCCTCCTTC 
CCATCGCCGC AGCGTTTCAG CCAGTCCGGG CAGAGCTACG ATGGCAGTTA CAGTGTGAAC 
GCTGGATCAC AGTATGAAGG GCATAACGTG GGCTCCAACG CACAGGCTTA CGGAACACAA 
TCCAATTATA GCTATCAGCC TCAATCTATG AAGAATTTCG AACAGGCCAA GATTCCACCA 
GGGACCCAGC AGGGTCAGCA GCAGCAGGGC GGCGGCCGGC AGCAGCGGCA GCAGCACCCC 
CCTCGGCACG TGATGCAGTA TTCCAACGCC GCCACGAAGC TACCCCTGCA AAGTCAGGTC 
GGGCAGTACA GCCAGCCCGA GGTTCCCGTG AGGTCCCCCA TGCAGTTTCA CCAGAACTTC 
AGCCCCATCT CTAACCCTTC CCCAGCTGCC TCTGTGGTTC AGTCTCCAAG CTGTAGCTCT 
ACCCCATCTC CTCTCACGCA GAGCGGGGAG AACCTCCAGT GTGGGCAAGG CAATGTGCCA 
ATGGGCTCCA GAAACAGAAT CCTGCAGTTA ATGCCTCAGC TCAGCCCAAC CCCGTCAATG 
ATGCCCAGTC CCAACTCTCA CGCTGCGGGC TTCAAAGGGT TCGGGCTGGA AGGGGTGCCT 
GAAAAGCGGC TGACCGATCC CGGCTTGAGT AGTTTGAGTG CCCTGAGTAC TCAGGTGGCC 
AACCTTCCTA ATACGGTTCA GCACATGCTA CTTTCCGACG CCCTGACCCC TCAGAAGAAG 
ACCTCCAAGA GGCCTTCCTC GTCATCCAAG AAAACAGATA GCTGCCCAAA CTCGGAAGGC 
TCCTCACAGG CCGAGGAACA GCTGAAGTCC CCAATGGCAG AGTCGCTGGA TGGAGGCTGC 
TCCAGCAGTT CTGAGGATCA AGGCGAGAGG GTGAGGCAGC TGAGCGGCCA GAGCACAAGC 
TCGGACACCA CCTACAAGGG CGGGGCCTCG GAAAAGGCAG GCTCCTCACC AGCACAGGGC 
ACTCAGAACG AAGCCCCCCG ACTCAGCACC AGTCCCGGAG CCCGAGAAGA GACCGCCTCG 
CCGGGTGCCA AGGACACACC GCTGTCATCT GAGGGCAACC CGAAAGCCAA CGAGAAGGCA 
GTCGGGGTGA TTGTCTCCCG GGAAGCCATG ACAGGCCGGG TGGAAAAGCC TGGGGGGCAG 
GACAAGGGCT CCCAGGAGGA GGATCCTGCA GCCACACAGA GGCCGCCCAG CACCGGGGGG 
GCAAAGGAAG CCAGCCACGC ATCACTTCCA CAGCCAGAGC CGCTGGGAGG AGGCAGTAAA 
GGAAACAAGA GCGGAGACAG TAACTCCAAC CACAACGGGG AAGGGAATGG CCAGACCGGG 
CACCCCGCAG GGGGCTCTGG TTTCACAGGC AGGACTGAGC CCAGCAAATC TCCTGGAAGC 
CTGCGCTATA GTTACAAAGA CAGTTTTGGG TCAGCCGTAC CGAGAAACAT GAGTGGCTTT 
GCTCAGTATC CTACAGGACA AGATAAAGGG GACTTCACCA GCCACGGGGA GCGAAAGGGT 
AGAAATGAGA AGTTCCCCAG CCTCCTGCAG GAGGTGCTTC AGGGTTACCA CCACCACCCC 
GACAGGAGGT ACTCCCGGAG TTCTCAGGAG CACCAGGCTA TGGCTGGCAG CCTCGAAGGA 
GCCACAAGGC CTAACGTCTT AGTGAGTCAG ACCAATGAGT TGGCTAGCAG GGGCCTTCTG 
AACAAAAGCA TTGGGTCCCT GTTAGAAAAC CCCCACTGGG GTCCATGGGA AAGGAAGTCA 
AGTGGCACAG CTCCTGAGAT GAAACAGATC AATTTGGCTG ACTATCCAAT TCCCAGAAAG 
TTTGAAATAG AGCCTCCGTC ATCAGCCCAT GAGCCCGGGG GTTCCCTCTC TGAGAGAAGA 
TCAGTCATCT GTGACATTTC TCCACTAAGA CAGATTGTCA GAGACCCAGG GGCTCACTCG 
CTAGGACACA TGGGCGCCGA CACCAGACTC GGGAGGAATG AGCGTCTCAA TCTCAGTTTA 
AGTCAGTCAG TCATTCTTCC AGGTGGGCTG GTGTCCATGG AAACAAAGCT GAAATCCCAG 
AGTGGGCAGA TAAAAGAGGA GGACTTTGAA CAGTCCAAGT CCCAAGCTAG TTTCAACAAC 
AAGAAATCAG GAGACCACTG CCACCCTGCT AGCATCAAGC ACGAGTCCTA CCGAGGCAAC 
GCCAGCCCCG GAGCGGCGAC CCACGACTCC ATCTCAGACT ACGGCCCGCA GGACAGCAGA 
CCCACACCAA TGCGGCGAGT CCCTGGCAGA GTTGGCGGTC GGGAGGGCAT GAGGGGTCGG 
TCCCCTTCTC AGTATCATGA CTTTTCAGAA AAGTTGAAGA TGTCCCCTGG GAGGAGCAGA 
GGCCCAGGGG GCGACCCTCA TCACATGAAC CCACACATGA CCTTCTCCGA GAGGGCCAAC 
AGGAGTTCTT TGCACGCCCC CTTTTCTCCC AATTCAGAAA GCCTGGCCTC TGCTTATCAC 
ACAAACACAC GTGCTCATGC TTATGGGGAC CCCAGTGCAG GTTTGAATTC CCAGCTCCAT 
TACAAGAGAC AGATGTACCA ACAGCAGCAA GAGGAATATA AGGACTGGGG CGGCAGTTCT 
GCTCAGGGAG TGATCGCTGC GGCTCAGCAC AGGCAGGAGG GGCCCCGCAA GAGTCCACGG 
CAGCAGCAGT TTCTTGACCG AGTCCGGAGC CCCCTGAAGA ATGACAAAGA TGGCATGATG 
TACGGCCCAC CGGTGGGGAC GCACCATGAC CCCAGCGGCC AGGACGGCGG GCGCTGCCTC 
ATGTCCAGTG ATGGTCTTTC TAACAAAGGC ATTGAACTGA AGCACAGCTC CCAGAAGTTA 
CAGCAAGAGT CTTGCTGGGA TCTCTCTCGG CAGACTTCTC CAGCCAAAAG CAGCGGCCCC 
CCAGGAATGT CCAGTCAGAA AAGGTACGGA CCACCCCACG AGACCGACGG ACATGGGCTC 
ACAGAGTCTA CGCAGTCATC CAAACCGAGT AACGTTATGC TGAGACTTCC AGGTCAAGAG 
GATCATTCTT CTCAAAACCC CTTAATCATG AGGAGGCGTG TCCGTTCTTT TATCTCTCCC 
ATTCCCAGTA AGAGACAGTC ACAGGACGTG AAGAACAGCA ACTCTGAAGA TAAAGGGCGC 
CTCCTTCACC CGTCAAAAGA AGGCACTGAC AAAGCGTACA ATTCCTATGC CCATCTTTCT 
CACAGTCAGG AGATCAAGTC CATCCCTAAG AGGGAGTCCT CCAAGGACCT TCCGAGTCCA 
GATAGTAGGA ACTGCCCTGC GGTTACCCTT ACAAGTCCTG CTAAGACCAA AATCCTGCCC 
CCGAGGAAAG GCCGGGGGTT GAAACTGGAA GCTATCGTCC AGAAGATCAC ATCCCCAAAC 
ATTAGGAGGA GTGCGTCCTC GAACAGCGCA GAGGCCGGGG TAGACACGGT TACTCTTGAC 
GACATCCTGT CTTTGAAAAG CGGCCCTCCA GAAGGTGGGA GCGGTGCTGT TCCGGACGCT 
GAGCTGGAGA AGAGAAAAGG TGAGGTGATA GCTGAGCTGG CCTGTCCCGT AAGCCAGGAG 
CTGAGCGGAG AAAAGCCTCT GGCCCGGTCT TCGGAGGAGT GGCGCGGTGG TGGGGACGAC 
AAGGTGAAGA CGGAGACGCA CCCTGACGCT GTCGCTGCTG GGAAGGAGCC CCCTGGTGCC 
ATGGCATCCG CAACCTCACA GAAGCCTGGG AGTAACCAGG GGAGACCAGA CGGGCCCCTG 
GGCGGGACAG CACCTTTAAT CTTTCCTGAC TCGAAGAGCG CACCTCCAGT GGGCACGCTG 
GCTCCCGAGG CAAACCCCAA GGCTGAAGAG AAAGAGAGCG ATGCCGTGAC GATTTCCCCC 
AAACAGGAGG GCTTCCCCCC GAAGGGTTAC TTCCCCTCAG GAAAGAAGAA AGGGAGACCC 
ATTGGTAGCG TGAATAAGCA GAAGAAACAG CAGCAGCCTC CGCCGTCACC CCCGCAGCCC 
CCTNCCGCAC CCCGGGGCTC TGCTGATGGA GAGCCAAAGC CGAAAAAGCA GAGGCAGCGG 
AGGGAGCGCA GGAAGCCCGG GGCGCAGCCC AGGAAGCGGA GGGCCAAGCA GGCCGCGCCC 
ATCGTAGAGC CCCAGGAGCC GGAGATCAAG CTGAAGTACG CCACCCAGCC CCTGGACAAA 
ACCGACGCCA AGAACAAGTC TTTTTTCCCT TATATCCACG TAGTAAATAA GTGTGAACTT 
GGAGCCGTGT GTACAATCAT CAACGCCGAG GAGGAGGAGC AGACCAAATT GGTGAGGGGG 
AGGAAGGGCC AGCGGTCCCT GACGCCTCCG CCCAGCAGCA CCGAGAGCAA GGTTCTCCCA 
GCCTCGTCCT TCGTGCTGCA GGGCCCCGTG GTGACAGAGT CTTCTGTGAT GGGGCACCTG 
GTTTGCTGTC TGTGCGGCAA GTGGGCCAGT TACCGGAACA TGGGCGACCT CTTTGGACCC 
TTTTACCCCC AAGATTATGC AGCCACTCTC CCCAAGAATC CACCTCCCAA GAGGGCCGCG 
GAAACGCAGA GCAAGGTGAA GGTCCGGCAC AAAAGCGCTT CGAACGGCTC CAAGACGGAC 
ACTGAGGAGG AGGAAGAGCA GCAGCAGCAG AAGGAGCAGA GGAGCCTGGC TGCGCACCCC 
AGGTTTAAGC GGCGACACCG CTCGGAAGAC TGTGCCGGAG GCCCCCGGTC CCTGTCCAGG 
GGGCTCCCTT GTAAAAAAGC CACCACTGAG GGCAGCAGCG AAAAGACTGT TTTGGACTCA 
AAGCCCTCCG TGCCCACCAC TTCAGAAGGT GGCCCCGAGT TGGAGTTACA AATCCCTGAA 
CTACCTCTCG ACAGCAATGA ATTTTGGGTC CACGAGGGTT GTATTCTCTG GGCCAATGGA 
ATCTACCTGG TCTGTGGCCG GCTCTATGGC CTGCAGGAAG CGCTGGAAAT AGCCAGAGAG 
ATGAAATGTT CCCACTGCCA GGAGGCAGGT GCCACCTTGG GCTGCTACAA CAAAGGCTGC 
TCCTTCCGAT ACCATTACCC GTGTGCCATT GACGCAGATT GTTTGCTCCA CGAGGAGAAC 
TTCTCGGTGA GGTGCCCCAA GCACAAGGTG AGACTGTGGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_004007026.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004007075.3 Ovis aries transcription factor 20 (TCF20), transcript variant X2, mRNA.

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CloneID	OSm78788
Clone ID Related Accession (Same CDS sequence)	XM_004007026.3
Accession Version	XM_004007026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004007026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CCGCGATGGC CAGCGAGACC TCTGGCCATC AGGGCTACCA GGGCTTCAGG 
AAGGAGGCTG GAGACTTCTA CTACATGGCA GGCAACAAGG ACCCCGTGTC TGCAGGAACC 
CCGCAGCCTC CTCCACGAAG GCCTTCCGGG CCGGTGCAGA GCTACGGACC CCCCCAGGGG 
AGCAGCTTTG GCAATCAGTA TGGCAGTGAG GGTCACGTGG GCCAGTTTCA AGCACAGCAC 
TCTGCCCTTG GCGGTGTGTC TCATTACTCA CAGGATTACA CAGGGCCGTT CTCTCCGGGG 
AGCGCTCAGT ACCAGCAGCA GCCTTCCAGC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGACA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCCGCC 
AGCCAGCCGG CGTCCGGTGC CTCCCACCTG CAGCCCATGC AGCGGCCCTC AGCTCTGCCG 
GCCTCGGCTG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACTGCCG CCGCCTCCTC CTTCCCATCG CCGCAGCGTT TCAGCCAGTC CGGGCAGAGC 
TACGATGGCA GTTACAGTGT GAACGCTGGA TCACAGTATG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTACGGAAC ACAATCCAAT TATAGCTATC AGCCTCAGTC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGTC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCCGCCA CGAAGCTACC CCTGCAAAGT CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CATCTCCTCT CACGCAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCAATGATGC CCAGTCCCAA CTCTCACGCT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACTCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGATAGCTG CCCAAACTCG GAAGGCTCCT CACAGGCCGA GGAACAGCTG 
AAGTCCCCAA TGGCAGAGTC GCTGGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGCACTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CCGGAGCCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCGCTG 
TCATCTGAGG GCAACCCGAA AGCCAACGAG AAGGCAGTCG GGGTGATTGT CTCCCGGGAA 
GCCATGACAG GCCGGGTGGA AAAGCCTGGG GGGCAGGACA AGGGCTCCCA GGAGGAGGAT 
CCTGCAGCCA CACAGAGGCC GCCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCATCA 
CTTCCACAGC CAGAGCCGCT GGGAGGAGGC AGTAAAGGAA ACAAGAGCGG AGACAGTAAC 
TCCAACCACA ACGGGGAAGG GAATGGCCAG ACCGGGCACC CCGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CCGTACCGAG AAACATGAGT GGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TACTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCTATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG 
AGTCAGACCA ATGAGTTGGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGTCC ATGGGAAAGG AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCTGACTA TCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCGTCATCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCAGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTCGGGA GGAATGAGCG TCTCAATCTC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAGGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCAGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCCTACCGA GGCAACGCCA GCCCCGGAGC GGCAACCCAC 
GACTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCT 
GGCAGAGTTG GCGGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAACCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA CGCCCCCTTT 
TCTCCCAATT CAGAAAGCCT GGCCTCTGCT TATCACACAA ACACACGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGCGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCACAGGC AGGAGGGGCC CCGCAAGAGT CCACGGCAGC AGCAGTTTCT TGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTACG GCCCACCGGT GGGGACGCAC 
CATGACCCCA GCGGCCAGGA CGGCGGGCGC TGCCTCATGT CCAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CAGCTCCCAG AAGTTACAGC AAGAGTCTTG CTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAG TCAGAAAAGG 
TACGGACCAC CCCACGAGAC CGACGGACAT GGGCTCACAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GACGTGAAGA ACAGCAACTC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCG AGTCCAGATA GTAGGAACTG CCCTGCGGTT 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGTGC GTCCTCGAAC 
AGCGCAGAGG CCGGGGTAGA CACGGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG GACGCTGAGC TGGAGAAGAG AAAAGGTGAG 
GTGATAGCTG AGCTGGCCTG TCCCGTAAGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACGCTGTCG CTGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCGCAAC CTCACAGAAG 
CCTGGGAGTA ACCAGGGGAG ACCAGACGGG CCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCGA AGAGCGCACC TCCAGTGGGC ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGCGTGAA TAAGCAGAAG 
AAACAGCAGC AGCCCCCGCC GCCACCCCCG CAGCCCCCTC AGATACCAGA GGGCTCTGCT 
GATGGAGAGC CAAAGCCGAA AAAGCAGAGG CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG 
CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC GCGCCCATCG TAGAGCCCCA GGAGCCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GACAAAACCG ACGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGAGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACTGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGTGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGACG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004007075.3
CDS	40..6000
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_004007026.3
Organism	Ovis aries(sheep)
Definition	Ovis aries transcription factor 20 (TCF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004007026.3

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CCGCGATGGC CAGCGAGACC TCTGGCCATC AGGGCTACCA GGGCTTCAGG 
AAGGAGGCTG GAGACTTCTA CTACATGGCA GGCAACAAGG ACCCCGTGTC TGCAGGAACC 
CCGCAGCCTC CTCCACGAAG GCCTTCCGGG CCGGTGCAGA GCTACGGACC CCCCCAGGGG 
AGCAGCTTTG GCAATCAGTA TGGCAGTGAG GGTCACGTGG GCCAGTTTCA AGCACAGCAC 
TCTGCCCTTG GCGGTGTGTC TCATTACTCA CAGGATTACA CAGGGCCGTT CTCTCCGGGG 
AGCGCTCAGT ACCAGCAGCA GCCTTCCAGC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGACA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCCGCC 
AGCCAGCCGG CGTCCGGTGC CTCCCACCTG CAGCCCATGC AGCGGCCCTC AGCTCTGCCG 
GCCTCGGCTG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACTGCCG CCGCCTCCTC CTTCCCATCG CCGCAGCGTT TCAGCCAGTC CGGGCAGAGC 
TACGATGGCA GTTACAGTGT GAACGCTGGA TCACAGTATG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTACGGAAC ACAATCCAAT TATAGCTATC AGCCTCAGTC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGTC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCCGCCA CGAAGCTACC CCTGCAAAGT CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CATCTCCTCT CACGCAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCAATGATGC CCAGTCCCAA CTCTCACGCT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACTCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGATAGCTG CCCAAACTCG GAAGGCTCCT CACAGGCCGA GGAACAGCTG 
AAGTCCCCAA TGGCAGAGTC GCTGGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGCACTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CCGGAGCCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCGCTG 
TCATCTGAGG GCAACCCGAA AGCCAACGAG AAGGCAGTCG GGGTGATTGT CTCCCGGGAA 
GCCATGACAG GCCGGGTGGA AAAGCCTGGG GGGCAGGACA AGGGCTCCCA GGAGGAGGAT 
CCTGCAGCCA CACAGAGGCC GCCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCATCA 
CTTCCACAGC CAGAGCCGCT GGGAGGAGGC AGTAAAGGAA ACAAGAGCGG AGACAGTAAC 
TCCAACCACA ACGGGGAAGG GAATGGCCAG ACCGGGCACC CCGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CCGTACCGAG AAACATGAGT GGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TACTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCTATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG 
AGTCAGACCA ATGAGTTGGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGTCC ATGGGAAAGG AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCTGACTA TCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCGTCATCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCAGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTCGGGA GGAATGAGCG TCTCAATCTC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAGGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCAGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCCTACCGA GGCAACGCCA GCCCCGGAGC GGCAACCCAC 
GACTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCT 
GGCAGAGTTG GCGGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAACCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA CGCCCCCTTT 
TCTCCCAATT CAGAAAGCCT GGCCTCTGCT TATCACACAA ACACACGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGCGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCACAGGC AGGAGGGGCC CCGCAAGAGT CCACGGCAGC AGCAGTTTCT TGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTACG GCCCACCGGT GGGGACGCAC 
CATGACCCCA GCGGCCAGGA CGGCGGGCGC TGCCTCATGT CCAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CAGCTCCCAG AAGTTACAGC AAGAGTCTTG CTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAG TCAGAAAAGG 
TACGGACCAC CCCACGAGAC CGACGGACAT GGGCTCACAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GACGTGAAGA ACAGCAACTC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCG AGTCCAGATA GTAGGAACTG CCCTGCGGTT 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGTGC GTCCTCGAAC 
AGCGCAGAGG CCGGGGTAGA CACGGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG GACGCTGAGC TGGAGAAGAG AAAAGGTGAG 
GTGATAGCTG AGCTGGCCTG TCCCGTAAGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACGCTGTCG CTGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCGCAAC CTCACAGAAG 
CCTGGGAGTA ACCAGGGGAG ACCAGACGGG CCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCGA AGAGCGCACC TCCAGTGGGC ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGCGTGAA TAAGCAGAAG 
AAACAGCAGC AGCCCCCGCC GCCACCCCCG CAGCCCCCTC AGATACCAGA GGGCTCTGCT 
GATGGAGAGC CAAAGCCGAA AAAGCAGAGG CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG 
CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC GCGCCCATCG TAGAGCCCCA GGAGCCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GACAAAACCG ACGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGAGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACTGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGTGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGACG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015088670.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944156.1 Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.

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CloneID	OOv28226
Clone ID Related Accession (Same CDS sequence)	XM_015088670.1 , XM_015088671.1
Accession Version	XM_015088670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944156.1
CDS	159..6191
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEDEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015088670.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088670.1

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015088671.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944157.1 Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.

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CloneID	OOv28226
Clone ID Related Accession (Same CDS sequence)	XM_015088670.1 , XM_015088671.1
Accession Version	XM_015088671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944157.1
CDS	159..6191
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEDEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015088671.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088671.1

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CGGCCGCGAT GGCCAGCGAG ACCTCTGGCC ATCAGGGCTA CCAGGGCTTC 
AGGAAGGAGG CTGGAGACTT CTACTACATG GCAGGCAACA AGGACCCCGT GTCTGCAGGA 
ACCCCGCAGC CTCCTCCGCG AAGGCCTTCC GGGCCGGTGC AGAGCTACGG ACCCCCCCAG 
GGGAGCAGCT TTGGCAATCA GTATGGCAGT GAGGGTCACG TGGGCCAGTT TCAAGCACAG 
CACTCTGCCC TTGGCGGTGT GTCTCATTAC TCACAGGATT ACACAGGGCC GTTCTCTCCG 
GGGAGCGCTC AGTACCAGCA GCAGCCTTCC AGCCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAAGTGC AGCAGCTGCG ACAGCAGCTC TACCAGTCCC ATCAGCCCCT GCCGCAGGCC 
GCCAGCCAGC CGGCGTCCGG TGCCTCCCAC CTGCAGCCCA TGCAGCGGCC CTCAGCTCTG 
CCGGCCTCGG CTGCCGGCTA CCAGCTGAGA GTGGGGCAGT TTGGCCAGCA CTACCAGTCT 
TCTGCCACTG CCGCTGCCTC CTCCTTCCCA TCGCCGCAGC GTTTCAGCCA GTCCGGGCAG 
AGCTATGATG GCAGTTACAG TGTGAACGCT GGATCACAGT ATGAAGGGCA TAACGTGGGC 
TCCAACGCAC AGGCTTACGG AACACAATCC AATTATAGCT ATCAGCCTCA ATCTATGAAG 
AATTTCGAAC AGGCCAAGAT TCCACCAGGG ACCCAGCAGG GTCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCACC CCCCTCAGCA CGTGATGCAG 
TATTCCAACG CCGCCACGAA GCTACCCCTG CAAAGTCAGG TCGGGCAGTA CAGCCAGCCC 
GAGGTTCCCG TGAGGTCCCC CATGCAGTTT CACCAGAACT TCAGCCCCAT CTCTAACCCT 
TCCCCAGCTG CCTCTGTGGT TCAGTCTCCA AGCTGTAGCT CTACCCCATC TCCTCTCACG 
CAGAGCGGGG AGAACCTCCA GTGTGGGCAA GGCAATGTGC CAATGGGCTC CAGAAACAGA 
ATCCTGCAGT TAATGCCTCA GCTCAGCCCA ACCCCGTCAA TGATGCCCAG TCCCAACTCT 
CACGCTGCGG GCTTCAAAGG GTTCGGGCTG GAAGGGGTGC CTGAAAAGCG GCTGACCGAT 
CCCGGCTTGA GTAGTTTGAG TGCCCTGAGT ACTCAGGTGG CCAACCTTCC TAATACGGTT 
CAGCACATGC TACTTTCCGA CGCCCTGACC CCTCAGAAGA AGACCTCCAA GAGGCCTTCC 
TCGTCATCCA AGAAAACAGA TAGCTGCCCA AACTCGGAAG GCTCCTCACA GGCCGAGGAA 
CAGCTGAAGT CCCCAATGGC AGAGTCGCTG GATGGAGGCT GCTCCAGCAG TTCTGAGGAT 
CAAGGCGAGA GGGTGAGGCA GCTGAGCGGC CAGAGCACAA GCTCGGACAC CACCTACAAG 
GGCGGGGCCT CGGAAAAGGC AGGCTCCTCA CCAGCACAGG GCACTCAGAA CGAAGCCCCC 
CGACTCAGCA CCAGTCCCGG AGCCCGAGAA GAGACCGCCT CGCCGGGTGC CAAGGACACA 
CCGCTGTCAT CTGAGGGCAA CCCGAAAGCC AACGAGAAGG CAGTCGGGGT GATTGTCTCC 
CGGGAAGCCA TGACAGGCCG GGTGGAAAAG CCTGGGGGGC AGGACAAGGG CTCCCAGGAG 
GAGGATCCTG CAGCCACACA GAGGCCGCCC AGCACCGGGG GGGCAAAGGA AGCCAGCCAC 
GCATCACTTC CACAGCCAGA GCCGCTGGGA GGAGGCAGTA AAGGAAACAA GAGCGGAGAC 
AGTAACTCCA ACCACAACGG GGAAGGGAAT GGCCAGACCG GGCACCCCGC AGGGGGCTCT 
GGTTTCACAG GCAGGACTGA GCCCAGCAAA TCTCCTGGAA GCCTGCGCTA TAGTTACAAA 
GACAGTTTTG GGTCAGCCGT ACCGAGAAAC ATGAGTGGCT TTGCTCAGTA TCCTACAGGA 
CAAGATAAAG GGGACTTCAC CAGCCACGGG GAGCGAAAGG GTAGAAATGA GAAGTTCCCC 
AGCCTCCTGC AGGAGGTGCT TCAGGGTTAC CACCACCACC CCGACAGGAG GTACTCCCGG 
AGTTCTCAGG AGCACCAGGC TATGGCTGGC AGCCTCGAAG GAGCCACAAG GCCTAACGTC 
TTAGTGAGTC AGACCAATGA GTTGGCTAGC AGGGGCCTTC TGAACAAAAG CATTGGGTCC 
CTGTTAGAAA ACCCCCACTG GGGTCCATGG GAAAGGAAGT CAAGTGGCAC AGCTCCTGAG 
ATGAAACAGA TCAATTTGGC TGACTATCCA ATTCCCAGAA AGTTTGAAAT AGAGCCTCCG 
TCATCAGCCC ATGAGCCCGG GGGTTCCCTC TCTGAGAGAA GATCAGTCAT CTGTGACATT 
TCTCCACTAA GACAGATTGT CAGAGACCCA GGGGCTCACT CGCTAGGACA CATGGGCGCC 
GACACCAGAC TCGGGAGGAA TGAGCGTCTC AATCTCAGTT TAAGTCAGTC AGTCATTCTT 
CCAGGTGGGC TGGTGTCCAT GGAAACAAAG CTGAAATCCC AGAGTGGGCA GATAAAAGAG 
GAGGACTTTG AACAGTCCAA GTCCCAAGCT AGTTTCAACA ACAAGAAATC AGGAGACCAC 
TGCCACCCTG CTAGCATCAA GCACGAGTCC TACCGAGGCA ACGCCAGCCC CGGAGCGGCG 
ACCCACGACT CCATCTCAGA CTACGGCCCG CAGGACAGCA GACCCACACC AATGCGGCGA 
GTCCCTGGCA GAGTTGGCGG TCGGGAGGGC ATGAGGGGTC GGTCCCCTTC TCAGTATCAT 
GACTTTTCAG AAAAGTTGAA GATGTCCCCT GGGAGGAGCA GAGGCCCAGG GGGCGACCCT 
CATCACATGA ACCCACACAT GACCTTCTCC GAGAGGGCCA ACAGGAGTTC TTTGCACGCC 
CCCTTTTCTC CCAATTCAGA AAGCCTGGCC TCTGCTTATC ACACAAACAC ACGTGCTCAT 
GCTTATGGGG ACCCCAGTGC AGGTTTGAAT TCCCAGCTCC ATTACAAGAG ACAGATGTAC 
CAACAGCAGC AAGAGGAATA TAAGGACTGG GGCGGCAGTT CTGCTCAGGG AGTGATCGCT 
GCGGCTCAGC ACAGGCAGGA GGGGCCCCGC AAGAGTCCAC GGCAGCAGCA GTTTCTTGAC 
CGAGTCCGGA GCCCCCTGAA GAATGACAAA GATGGCATGA TGTACGGCCC ACCGGTGGGG 
ACGCACCATG ACCCCAGCGG CCAGGACGGC GGGCGCTGCC TCATGTCCAG TGATGGTCTT 
TCTAACAAAG GCATTGAACT GAAGCACAGC TCCCAGAAGT TACAGCAAGA GTCTTGCTGG 
GATCTCTCTC GGCAGACTTC TCCAGCCAAA AGCAGCGGCC CCCCAGGAAT GTCCAGTCAG 
AAAAGGTACG GACCACCCCA CGAGACCGAC GGACATGGGC TCACAGAGTC TACGCAGTCA 
TCCAAACCGA GTAACGTTAT GCTGAGACTT CCAGGTCAAG AGGATCATTC TTCTCAAAAC 
CCCTTAATCA TGAGGAGGCG TGTCCGTTCT TTTATCTCTC CCATTCCCAG TAAGAGACAG 
TCACAGGACG TGAAGAACAG CAACTCTGAA GATAAAGGGC GCCTCCTTCA CCCGTCAAAA 
GAAGGCACTG ACAAAGCGTA CAATTCCTAT GCCCATCTTT CTCACAGTCA GGAGATCAAG 
TCCATCCCTA AGAGGGAGTC CTCCAAGGAC CTTCCGAGTC CAGATAGTAG GAACTGCCCT 
GCGGTTACCC TTACAAGTCC TGCTAAGACC AAAATCCTGC CCCCGAGGAA AGGCCGGGGG 
TTGAAACTGG AAGCTATCGT CCAGAAGATC ACATCCCCAA ACATTAGGAG GAGTGCGTCC 
TCGAACAGCG CAGAGGCCGG GGTAGACACG GTTACTCTTG ACGACATCCT GTCTTTGAAA 
AGCGGCCCTC CAGAAGGTGG GAGCGGTGCT GTTCCGGACG CTGAGCTGGA GAAGAGAAAA 
GGTGAGGTGA TAGCTGAGCT GGCCTGTCCC GTAAGCCAGG AGCTGAGCGG AGAAAAGCCT 
CTGGCCCGGT CTTCGGAGGA GTGGCGCGGT GGTGGGGACG ACAAGGTGAA GACGGAGACG 
CACCCTGACG CTGTCGCTGC TGGGAAGGAG CCCCCTGGTG CCATGGCATC CGCAACCTCA 
CAGAAGCCTG GGAGTAACCA GGGGAGACCA GACGGGCCCC TGGGCGGGAC AGCACCTTTA 
ATCTTTCCTG ACTCGAAGAG CGCACCTCCA GTGGGCACGC TGGCTCCCGA GGCAAACCCC 
AAGGCTGAAG AGAAAGAGAG CGATGCCGTG ACGATTTCCC CCAAACAGGA GGGCTTCCCC 
CCGAAGGGTT ACTTCCCCTC AGGAAAGAAG AAAGGGAGAC CCATTGGTAG CGTGAATAAG 
CAGAAGAAAC AGCAGCAGCC CCCGCCGCCA CCCCCGCAGC CCCCTCAGAT ACCAGAGGGC 
TCTGCTGATG GAGAGCCAAA GCCGAAAAAG CAGAGGCAGC GGAGGGAGCG CAGGAAGCCC 
GGGGCGCAGC CCAGGAAGCG GAGGGCCAAG CAGGCCGCGC CCATCGTAGA GCCCCAGGAG 
CCGGAGATCA AGCTGAAGTA CGCCACCCAG CCCCTGGACA AAACCGACGC CAAGAACAAG 
TCTTTTTTCC CTTATATCCA CGTAGTAAAT AAGTGTGAAC TTGGAGCCGT GTGTACAATC 
ATCAACGCCG AGGAGGAGGA GCAGACCAAA TTGGTGAGGG GGAGGAAGGG CCAGCGGTCC 
CTGACGCCTC CGCCCAGCAG CACCGAGAGC AAGGTTCTCC CAGCCTCGTC CTTCGTGCTG 
CAGGGCCCCG TGGTGACAGA GTCTTCTGTG ATGGGGCACC TGGTTTGCTG TCTGTGCGGC 
AAGTGGGCCA GTTACCGGAA CATGGGCGAC CTCTTTGGAC CCTTTTACCC CCAAGATTAT 
GCAGCCACTC TCCCCAAGAA TCCACCTCCC AAGAGGGCCG CGGAAACGCA GAGCAAGGTG 
AAGGTCCGGC ACAAAAGCGC TTCGAACGGC TCCAAGACGG ACACTGAGGA GGAGGACGAG 
CAGCAGCAGC AGAAGGAGCA GAGGAGCCTG GCTGCGCACC CCAGGTTTAA GCGGCGACAC 
CGCTCGGAAG ACTGTGCCGG AGGCCCCCGG TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA 
GCCACCACTG AGGGCAGCAG CGAAAAGACT GTTTTGGACT CAAAGCCCTC CGTGCCCACC 
ACTTCAGAAG GTGGCCCCGA GTTGGAGTTA CAAATCCCTG AACTACCTCT CGACAGCAAT 
GAATTTTGGG TCCACGAGGG TTGTATTCTC TGGGCCAATG GAATCTACCT GGTCTGTGGC 
CGGCTCTATG GCCTGCAGGA AGCGCTGGAA ATAGCCAGAG AGATGAAATG TTCCCACTGC 
CAGGAGGCAG GTGCCACCTT GGGCTGCTAC AACAAAGGCT GCTCCTTCCG ATACCATTAC 
CCGTGTGCCA TTGACGCAGA TTGTTTGCTC CACGAGGAGA ACTTCTCGGT GAGGTGCCCC 
AAGCACAAGC CTCCCCTCCC ATGCCCTGTC CCCCCCTTGC AGAACAAGAC AGCGAAAGGC 
AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_027968172.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027823973.1 Ovis aries transcription factor 20 (TCF20), transcript variant X1, mRNA.

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CloneID	OSm78787
Clone ID Related Accession (Same CDS sequence)	XM_027968172.1
Accession Version	XM_027968172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CCGCGATGGC CAGCGAGACC TCTGGCCATC AGGGCTACCA GGGCTTCAGG 
AAGGAGGCTG GAGACTTCTA CTACATGGCA GGCAACAAGG ACCCCGTGTC TGCAGGAACC 
CCGCAGCCTC CTCCACGAAG GCCTTCCGGG CCGGTGCAGA GCTACGGACC CCCCCAGGGG 
AGCAGCTTTG GCAATCAGTA TGGCAGTGAG GGTCACGTGG GCCAGTTTCA AGCACAGCAC 
TCTGCCCTTG GCGGTGTGTC TCATTACTCA CAGGATTACA CAGGGCCGTT CTCTCCGGGG 
AGCGCTCAGT ACCAGCAGCA GCCTTCCAGC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGACA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCCGCC 
AGCCAGCCGG CGTCCGGTGC CTCCCACCTG CAGCCCATGC AGCGGCCCTC AGCTCTGCCG 
GCCTCGGCTG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACTGCCG CCGCCTCCTC CTTCCCATCG CCGCAGCGTT TCAGCCAGTC CGGGCAGAGC 
TACGATGGCA GTTACAGTGT GAACGCTGGA TCACAGTATG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTACGGAAC ACAATCCAAT TATAGCTATC AGCCTCAGTC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGTC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCCGCCA CGAAGCTACC CCTGCAAAGT CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CATCTCCTCT CACGCAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCAATGATGC CCAGTCCCAA CTCTCACGCT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACTCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGATAGCTG CCCAAACTCG GAAGGCTCCT CACAGGCCGA GGAACAGCTG 
AAGTCCCCAA TGGCAGAGTC GCTGGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGCACTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CCGGAGCCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCGCTG 
TCATCTGAGG GCAACCCGAA AGCCAACGAG AAGGCAGTCG GGGTGATTGT CTCCCGGGAA 
GCCATGACAG GCCGGGTGGA AAAGCCTGGG GGGCAGGACA AGGGCTCCCA GGAGGAGGAT 
CCTGCAGCCA CACAGAGGCC GCCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCATCA 
CTTCCACAGC CAGAGCCGCT GGGAGGAGGC AGTAAAGGAA ACAAGAGCGG AGACAGTAAC 
TCCAACCACA ACGGGGAAGG GAATGGCCAG ACCGGGCACC CCGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CCGTACCGAG AAACATGAGT GGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TACTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCTATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG 
AGTCAGACCA ATGAGTTGGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGTCC ATGGGAAAGG AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCTGACTA TCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCGTCATCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCAGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTCGGGA GGAATGAGCG TCTCAATCTC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAGGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCAGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCCTACCGA GGCAACGCCA GCCCCGGAGC GGCAACCCAC 
GACTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCT 
GGCAGAGTTG GCGGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAACCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA CGCCCCCTTT 
TCTCCCAATT CAGAAAGCCT GGCCTCTGCT TATCACACAA ACACACGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGCGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCACAGGC AGGAGGGGCC CCGCAAGAGT CCACGGCAGC AGCAGTTTCT TGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTACG GCCCACCGGT GGGGACGCAC 
CATGACCCCA GCGGCCAGGA CGGCGGGCGC TGCCTCATGT CCAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CAGCTCCCAG AAGTTACAGC AAGAGTCTTG CTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAG TCAGAAAAGG 
TACGGACCAC CCCACGAGAC CGACGGACAT GGGCTCACAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GACGTGAAGA ACAGCAACTC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCG AGTCCAGATA GTAGGAACTG CCCTGCGGTT 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGTGC GTCCTCGAAC 
AGCGCAGAGG CCGGGGTAGA CACGGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG GACGCTGAGC TGGAGAAGAG AAAAGGTGAG 
GTGATAGCTG AGCTGGCCTG TCCCGTAAGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACGCTGTCG CTGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCGCAAC CTCACAGAAG 
CCTGGGAGTA ACCAGGGGAG ACCAGACGGG CCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCGA AGAGCGCACC TCCAGTGGGC ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGCGTGAA TAAGCAGAAG 
AAACAGCAGC AGCCCCCGCC GCCACCCCCG CAGCCCCCTC AGATACCAGA GGGCTCTGCT 
GATGGAGAGC CAAAGCCGAA AAAGCAGAGG CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG 
CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC GCGCCCATCG TAGAGCCCCA GGAGCCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GACAAAACCG ACGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGAGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACTGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGTGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGACG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027823973.1
CDS	40..6066
Translation	MSPPSLNLALFVLGRCVSNASLFSFWFQEGCWPAAALLSSMQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSGGSSSGGGRRATAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVSAGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSALPASAAGYQLRVGQFGQHYQSSATAAASSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGTQNEAPRLSTSPGAREETASPGAKDTPLSSEGNPKANEKAVGVIVSREAMTGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPLGGGSKGNKSGDSNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNMSGFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNLSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTHHDPSGQDGGRCLMSSDGLSNKGIELKHSSQKLQQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLTESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNSEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGVDTVTLDDILSLKSGPPEGGSGAVPDAELEKRKGEVIAELACPVSQELSGEKPLARSSEEWRGGGDDKVKTETHPDAVAAGKEPPGAMASATSQKPGSNQGRPDGPLGGTAPLIFPDSKSAPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRRAKQAAPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_027968172.1
Organism	Ovis aries(sheep)
Definition	Ovis aries transcription factor 20 (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027968172.1

ORF Insert Sequence:

ATGTCCCCTC CATCTCTAAA CTTGGCTTTG TTTGTCCTCG GGAGATGCGT GTCTAATGCT 
TCTCTTTTCT CCTTCTGGTT CCAGGAGGGC TGCTGGCCTG CTGCTGCGCT GCTGAGCAGT 
ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCGGCGGC AGCAGCAGCG GTGGTGGACG ACGAGCAACG 
GCGGCGGCGG CCGCGATGGC CAGCGAGACC TCTGGCCATC AGGGCTACCA GGGCTTCAGG 
AAGGAGGCTG GAGACTTCTA CTACATGGCA GGCAACAAGG ACCCCGTGTC TGCAGGAACC 
CCGCAGCCTC CTCCACGAAG GCCTTCCGGG CCGGTGCAGA GCTACGGACC CCCCCAGGGG 
AGCAGCTTTG GCAATCAGTA TGGCAGTGAG GGTCACGTGG GCCAGTTTCA AGCACAGCAC 
TCTGCCCTTG GCGGTGTGTC TCATTACTCA CAGGATTACA CAGGGCCGTT CTCTCCGGGG 
AGCGCTCAGT ACCAGCAGCA GCCTTCCAGC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGACA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCCGCC 
AGCCAGCCGG CGTCCGGTGC CTCCCACCTG CAGCCCATGC AGCGGCCCTC AGCTCTGCCG 
GCCTCGGCTG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACTGCCG CCGCCTCCTC CTTCCCATCG CCGCAGCGTT TCAGCCAGTC CGGGCAGAGC 
TACGATGGCA GTTACAGTGT GAACGCTGGA TCACAGTATG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTACGGAAC ACAATCCAAT TATAGCTATC AGCCTCAGTC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGTC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCCGCCA CGAAGCTACC CCTGCAAAGT CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCTG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CATCTCCTCT CACGCAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCAATGATGC CCAGTCCCAA CTCTCACGCT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACTCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGATAGCTG CCCAAACTCG GAAGGCTCCT CACAGGCCGA GGAACAGCTG 
AAGTCCCCAA TGGCAGAGTC GCTGGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG CGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGCACTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CCGGAGCCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCGCTG 
TCATCTGAGG GCAACCCGAA AGCCAACGAG AAGGCAGTCG GGGTGATTGT CTCCCGGGAA 
GCCATGACAG GCCGGGTGGA AAAGCCTGGG GGGCAGGACA AGGGCTCCCA GGAGGAGGAT 
CCTGCAGCCA CACAGAGGCC GCCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCATCA 
CTTCCACAGC CAGAGCCGCT GGGAGGAGGC AGTAAAGGAA ACAAGAGCGG AGACAGTAAC 
TCCAACCACA ACGGGGAAGG GAATGGCCAG ACCGGGCACC CCGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CCGTACCGAG AAACATGAGT GGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TACTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCTATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA CGTCTTAGTG 
AGTCAGACCA ATGAGTTGGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGTCC ATGGGAAAGG AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCTGACTA TCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCGTCATCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCAGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTCGGGA GGAATGAGCG TCTCAATCTC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAGGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCAGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCCTACCGA GGCAACGCCA GCCCCGGAGC GGCAACCCAC 
GACTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCT 
GGCAGAGTTG GCGGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAACCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA CGCCCCCTTT 
TCTCCCAATT CAGAAAGCCT GGCCTCTGCT TATCACACAA ACACACGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGCGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCACAGGC AGGAGGGGCC CCGCAAGAGT CCACGGCAGC AGCAGTTTCT TGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTACG GCCCACCGGT GGGGACGCAC 
CATGACCCCA GCGGCCAGGA CGGCGGGCGC TGCCTCATGT CCAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CAGCTCCCAG AAGTTACAGC AAGAGTCTTG CTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAG TCAGAAAAGG 
TACGGACCAC CCCACGAGAC CGACGGACAT GGGCTCACAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GACGTGAAGA ACAGCAACTC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCG AGTCCAGATA GTAGGAACTG CCCTGCGGTT 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCGA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGTGC GTCCTCGAAC 
AGCGCAGAGG CCGGGGTAGA CACGGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCAGAAG GTGGGAGCGG TGCTGTTCCG GACGCTGAGC TGGAGAAGAG AAAAGGTGAG 
GTGATAGCTG AGCTGGCCTG TCCCGTAAGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACGCTGTCG CTGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCGCAAC CTCACAGAAG 
CCTGGGAGTA ACCAGGGGAG ACCAGACGGG CCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCGA AGAGCGCACC TCCAGTGGGC ACGCTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGCGTGAA TAAGCAGAAG 
AAACAGCAGC AGCCCCCGCC GCCACCCCCG CAGCCCCCTC AGATACCAGA GGGCTCTGCT 
GATGGAGAGC CAAAGCCGAA AAAGCAGAGG CAGCGGAGGG AGCGCAGGAA GCCCGGGGCG 
CAGCCCAGGA AGCGGAGGGC CAAGCAGGCC GCGCCCATCG TAGAGCCCCA GGAGCCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GACAAAACCG ACGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGAGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCTGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACTGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGTGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGACG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TCF20 cDNA ORF clone, Ovis aries(sheep)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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