MYO5A gene cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

mRNA	Protein	Name
XM_012118657.2	XP_011974047.1	unconventional myosin-Va isoform X8
XM_015097038.1	XP_014952524.1	unconventional myosin-Va isoform X5
XM_015097033.1	XP_014952519.1	unconventional myosin-Va isoform X1
XM_015097033.2	XP_014952519.1	unconventional myosin-Va isoform X1
XM_015097040.1	XP_014952526.1	unconventional myosin-Va isoform X7
XM_015097035.1	XP_014952521.1	unconventional myosin-Va isoform X2
XM_015097041.2	XP_014952527.1	unconventional myosin-Va isoform X5
XM_012118649.2	XP_011974039.1	unconventional myosin-Va isoform X1
XM_012118650.2	XP_011974040.1	unconventional myosin-Va isoform X2
XM_012118653.2	XP_011974043.1	unconventional myosin-Va isoform X5
XM_012118655.2	XP_011974045.1	unconventional myosin-Va isoform X6
XM_012118656.2	XP_011974046.1	unconventional myosin-Va isoform X7
XM_015097035.2	XP_014952521.1	unconventional myosin-Va isoform X2
XM_015097036.1	XP_014952522.1	unconventional myosin-Va isoform X3
XM_015097037.1	XP_014952523.1	unconventional myosin-Va isoform X4
XM_015097039.2	XP_014952525.1	unconventional myosin-Va isoform X4
XM_015097041.1	XP_014952527.1	unconventional myosin-Va isoform X8
XM_015097038.2	XP_014952524.1	unconventional myosin-Va isoform X3
XM_015097039.1	XP_014952525.1	unconventional myosin-Va isoform X6
XM_012118651.2	XP_011974041.1	unconventional myosin-Va isoform X3
XM_012118652.2	XP_011974042.1	unconventional myosin-Va isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv06417	XM_012118649.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06418	XM_012118650.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06419	XM_012118651.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06420	XM_012118652.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06421	XM_012118653.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06422	XM_012118655.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06423	XM_012118656.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv06424	XM_012118657.2 Latest version!	Ovis aries musimon myosin VA (MYO5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50440	XM_015097033.2 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50441	XM_015097035.2 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50442	XM_015097036.1 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50443	XM_015097037.1 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50444	XM_015097038.2 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50445	XM_015097039.2 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50446	XM_015097040.1 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50447	XM_015097041.2 Latest version!	Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSm50440	XM_015097033.1	Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50441	XM_015097035.1	Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50447	XM_015097041.1	Ovis aries myosin VA (MYO5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50444	XM_015097038.1	Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm50445	XM_015097039.1	Ovis aries myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

MYO5A ( XM_012118649.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974039.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv06417
Clone ID Related Accession (Same CDS sequence)	XM_012118649.2
Accession Version	XM_012118649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA TTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974039.1
CDS	124..5766
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118649.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118649.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA TTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118650.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974040.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OOv06418
Clone ID Related Accession (Same CDS sequence)	XM_012118650.2
Accession Version	XM_012118650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ATTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974040.1
CDS	124..5757
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118650.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118650.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ATTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118651.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974041.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OOv06419
Clone ID Related Accession (Same CDS sequence)	XM_012118651.2
Accession Version	XM_012118651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTTA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC GTATCACCAG GACAGATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AGCTGAAGCC ACGTGGTGTG 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCTTGT TTATGTGTGT TCGGCATGCT 
GACTACCTGA ATGATGACCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAGAAAGTAC TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCCAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCGA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG CACCTACACA 
CTGGACTCCA TCCTGCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCGTGGG GGCCGTCACC 
CTCAACAACC TCCTCTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAATTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CAGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCGGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT ACTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACT 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974041.1
CDS	124..5691
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118651.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118651.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTTA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC GTATCACCAG GACAGATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AGCTGAAGCC ACGTGGTGTG 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCTTGT TTATGTGTGT TCGGCATGCT 
GACTACCTGA ATGATGACCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAGAAAGTAC TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCCAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCGA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG CACCTACACA 
CTGGACTCCA TCCTGCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCGTGGG GGCCGTCACC 
CTCAACAACC TCCTCTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAATTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CAGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCGGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT ACTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACT 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118652.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974042.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv06420
Clone ID Related Accession (Same CDS sequence)	XM_012118652.2
Accession Version	XM_012118652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTTAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACGTATCA CCAGGACAGA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAGCTGA AGCCACGTGG TGTGGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC TTGTTTATGT GTGTTCGGCA TGCTGACTAC 
CTGAATGATG ACCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAACAG CATCAAGAAA 
GTACTGAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC CAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGCGGG TGTTAGAGAA CATCCTTCAG 
CCGATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGCACCTA CACACTGGAC 
TCCATCCTGC GGCAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAACTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATCG TGGGGGCCGT CACCCTCAAC 
AACCTCCTCT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAT TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCAGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCGGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTACTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACTATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974042.1
CDS	124..5685
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118652.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118652.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTTAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACGTATCA CCAGGACAGA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAGCTGA AGCCACGTGG TGTGGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC TTGTTTATGT GTGTTCGGCA TGCTGACTAC 
CTGAATGATG ACCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAACAG CATCAAGAAA 
GTACTGAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC CAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGCGGG TGTTAGAGAA CATCCTTCAG 
CCGATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGCACCTA CACACTGGAC 
TCCATCCTGC GGCAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAACTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATCG TGGGGGCCGT CACCCTCAAC 
AACCTCCTCT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAT TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCAGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCGGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTACTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACTATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118653.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974043.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv06421
Clone ID Related Accession (Same CDS sequence)	XM_012118653.2
Accession Version	XM_012118653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAATTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974043.1
CDS	124..5682
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118653.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118653.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAATTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118655.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974045.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OOv06422
Clone ID Related Accession (Same CDS sequence)	XM_012118655.2
Accession Version	XM_012118655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
TTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974045.1
CDS	124..5676
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118655.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118655.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
TTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118656.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974046.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X7, mRNA.

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CloneID	OOv06423
Clone ID Related Accession (Same CDS sequence)	XM_012118656.2
Accession Version	XM_012118656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTTAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACG TATCACCAGG ACAGATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA GCTGAAGCCA CGTGGTGTGG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCTTGTT TATGTGTGTT CGGCATGCTG ACTACCTGAA TGATGACCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AGAAAGTACT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCCAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCGAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGG GCCGTCACCC TCAACAACCT CCTCTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAATTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC AGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCGGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTA CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACTA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974046.1
CDS	124..5610
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118656.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118656.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTTAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACG TATCACCAGG ACAGATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA GCTGAAGCCA CGTGGTGTGG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCTTGTT TATGTGTGTT CGGCATGCTG ACTACCTGAA TGATGACCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AGAAAGTACT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCCAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCGAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGG GCCGTCACCC TCAACAACCT CCTCTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAATTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC AGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCGGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTA CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACTA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_012118657.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011974047.1 Ovis aries musimon myosin VA (MYO5A), transcript variant X8, mRNA.

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CloneID	OOv06424
Clone ID Related Accession (Same CDS sequence)	XM_012118657.2
Accession Version	XM_012118657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942272.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAATTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974047.1
CDS	124..5601
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNKYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_012118657.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon myosin VA (MYO5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118657.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAAGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAATTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097033.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952519.1 Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.

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CloneID	OSm50440
Clone ID Related Accession (Same CDS sequence)	XM_015097033.1 , XM_015097033.2
Accession Version	XM_015097033.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952519.1
CDS	18..5660
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097033.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097033.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097033.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952519.1 Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.

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CloneID	OSm50440
Clone ID Related Accession (Same CDS sequence)	XM_015097033.1 , XM_015097033.2
Accession Version	XM_015097033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015097033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952519.1
CDS	69..5711
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097033.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097033.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTTAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACGTATC ACCAGGACAG 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAGCTG 
AAGCCACGTG GTGTGGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CTTGTTTATG 
TGTGTTCGGC ATGCTGACTA CCTGAATGAT GACCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAGAA AGTACTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CCAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCGATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGCACCT ACACACTGGA CTCCATCCTG CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
GTGGGGGCCG TCACCCTCAA CAACCTCCTC TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCAGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCGG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTACTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACTATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097035.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952521.1 Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OSm50441
Clone ID Related Accession (Same CDS sequence)	XM_015097035.1 , XM_015097035.2
Accession Version	XM_015097035.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952521.1
CDS	18..5651
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097035.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097035.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097035.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952521.1 Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OSm50441
Clone ID Related Accession (Same CDS sequence)	XM_015097035.1 , XM_015097035.2
Accession Version	XM_015097035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015097035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952521.1
CDS	69..5702
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097035.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097035.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTTAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACGTAT CACCAGGACA GATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAGCT GAAGCCACGT 
GGTGTGGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCTTGTTTAT GTGTGTTCGG 
CATGCTGACT ACCTGAATGA TGACCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAGA AAGTACTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCCAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCGATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGCACC 
TACACACTGG ACTCCATCCT GCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CGTGGGGGCC 
GTCACCCTCA ACAACCTCCT CTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCAGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCG GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTACTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACTATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097036.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952522.1 Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OSm50442
Clone ID Related Accession (Same CDS sequence)	XM_015097036.1
Accession Version	XM_015097036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTTA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC GTATCACCAG GACAGATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AGCTGAAGCC ACGTGGTGTG 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCTTGT TTATGTGTGT TCGGCATGCT 
GACTACCTGA ATGATGACCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAGAAAGTAC TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCCAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCGA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG CACCTACACA 
CTGGACTCCA TCCTGCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCGTGGG GGCCGTCACC 
CTCAACAACC TCCTCTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CAGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCGGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT ACTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACT 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952522.1
CDS	18..5585
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097036.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097036.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
TCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGCGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTTA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC GTATCACCAG GACAGATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AGCTGAAGCC ACGTGGTGTG 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCTTGT TTATGTGTGT TCGGCATGCT 
GACTACCTGA ATGATGACCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAGAAAGTAC TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCCAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCGA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG CACCTACACA 
CTGGACTCCA TCCTGCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCGTGGG GGCCGTCACC 
CTCAACAACC TCCTCTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CAGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCGGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT ACTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACT 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097037.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952523.1 Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OSm50443
Clone ID Related Accession (Same CDS sequence)	XM_015097037.1
Accession Version	XM_015097037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTTAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACGTATCA CCAGGACAGA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAGCTGA AGCCACGTGG TGTGGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC TTGTTTATGT GTGTTCGGCA TGCTGACTAC 
CTGAATGATG ACCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAACAG CATCAAGAAA 
GTACTGAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC CAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGCGGG TGTTAGAGAA CATCCTTCAG 
CCGATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGCACCTA CACACTGGAC 
TCCATCCTGC GGCAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAACTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATCG TGGGGGCCGT CACCCTCAAC 
AACCTCCTCT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCAGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCGGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTACTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACTATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952523.1
CDS	18..5579
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097037.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097037.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTTAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACGTATCA CCAGGACAGA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAGCTGA AGCCACGTGG TGTGGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC TTGTTTATGT GTGTTCGGCA TGCTGACTAC 
CTGAATGATG ACCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAACAG CATCAAGAAA 
GTACTGAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC CAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGCGGG TGTTAGAGAA CATCCTTCAG 
CCGATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGCACCTA CACACTGGAC 
TCCATCCTGC GGCAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAACTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATCG TGGGGGCCGT CACCCTCAAC 
AACCTCCTCT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCAGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCGGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTACTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACTATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097038.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952524.1 Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OSm50444
Clone ID Related Accession (Same CDS sequence)	XM_015097038.1 , XM_015097038.2
Accession Version	XM_015097038.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952524.1
CDS	18..5576
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097038.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097038.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097038.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952524.1 Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OSm50444
Clone ID Related Accession (Same CDS sequence)	XM_015097038.1 , XM_015097038.2
Accession Version	XM_015097038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015097038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952524.1
CDS	69..5627
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097038.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097038.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ATCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGCGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTT AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CGTATCACCA GGACAGATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAGCTGAAGC CACGTGGTGT GGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCTTG TTTATGTGTG TTCGGCATGC TGACTACCTG 
AATGATGACC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAGAAAGTA 
CTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCCAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCG 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GCACCTACAC ACTGGACTCC 
ATCCTGCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCGTGG GGGCCGTCAC CCTCAACAAC 
CTCCTCTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCAGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCGGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TACTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC TATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097039.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952525.1 Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OSm50445
Clone ID Related Accession (Same CDS sequence)	XM_015097039.2 , XM_015097039.1
Accession Version	XM_015097039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015097039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952525.1
CDS	69..5621
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097039.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097039.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097039.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952525.1 Ovis aries myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OSm50445
Clone ID Related Accession (Same CDS sequence)	XM_015097039.2 , XM_015097039.1
Accession Version	XM_015097039.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952525.1
CDS	18..5570
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097039.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097039.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTTAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACGTATC ACCAGGACAG ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAGCTG AAGCCACGTG GTGTGGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CTTGTTTATG TGTGTTCGGC ATGCTGACTA CCTGAATGAT 
GACCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAACA GCATCAAGAA AGTACTGAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CCAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGCGG GTGTTAGAGA ACATCCTTCA GCCGATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGCACCT ACACACTGGA CTCCATCCTG 
CGGCAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAACTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATC GTGGGGGCCG TCACCCTCAA CAACCTCCTC 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCAGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCGG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTACTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACTATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097040.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952526.1 Ovis aries myosin VA (MYO5A), transcript variant X7, mRNA.

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CloneID	OSm50446
Clone ID Related Accession (Same CDS sequence)	XM_015097040.1
Accession Version	XM_015097040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTTAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACG TATCACCAGG ACAGATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA GCTGAAGCCA CGTGGTGTGG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCTTGTT TATGTGTGTT CGGCATGCTG ACTACCTGAA TGATGACCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AGAAAGTACT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCCAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCGAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGG GCCGTCACCC TCAACAACCT CCTCTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC AGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCGGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTA CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACTA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952526.1
CDS	18..5504
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097040.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097040.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGCGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTTAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACG TATCACCAGG ACAGATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA GCTGAAGCCA CGTGGTGTGG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCTTGTT TATGTGTGTT CGGCATGCTG ACTACCTGAA TGATGACCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AGAAAGTACT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCCAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCGAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGG GCCGTCACCC TCAACAACCT CCTCTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC AGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCGGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTA CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACTA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097041.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952527.1 Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OSm50447
Clone ID Related Accession (Same CDS sequence)	XM_015097041.2 , XM_015097041.1
Accession Version	XM_015097041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015097041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAACTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952527.1
CDS	69..5546
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097041.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097041.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAACTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015097041.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952527.1 Ovis aries myosin VA (MYO5A), transcript variant X8, mRNA.

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CloneID	OSm50447
Clone ID Related Accession (Same CDS sequence)	XM_015097041.2 , XM_015097041.1
Accession Version	XM_015097041.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAACTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952527.1
CDS	18..5495
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASANLPEFKALRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVAFEEMSHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAVSPTSATSSGRTPLTRTPSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTAAIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTYYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGTYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTRSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSSPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015097041.1
Organism	Ovis aries(sheep)
Definition	Ovis aries myosin VA (MYO5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015097041.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTTCTCCTT 
CACCTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGACC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAA AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGCGGAG AGTCTGGGGC CGGAAAAACA GTCTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTC 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAAAC CTACCTGAAT TTAAAGCGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAA GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
TATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGC CTTTGAGGAG ATGAGTCACT GGCTCTGCCA TCGGAAGCTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCACTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAGCAGCAT TCTTTTATCG GTGTGCTGGA TATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GTTACCAGAA TTATTTCAAG ACGATGAGAA GGCCGTCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCCTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC AGCCAAAGAG CACAAGAAAA CAGTAGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AACGCCACTA CACCTCACTA TGTACGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATT CGGATCAGCG CGGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTACTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACCGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGTAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
GCAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGTGGGTCC GTGGCTGGCT GGCTCGCACC 
TACTATAGGA GGAGCATCCA CGCCATCATC TACCTCCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGCG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CCGTGGAGCG CTATAAGAAG 
CTCCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAACCTAG AAGGAACATA CAACTCGGAG 
ACCGAGAAGC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCGGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA GCAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGCC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTGACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCGAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTT CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGTGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATC 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA AAAAATGAAA 
GATAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGCGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGG ATTTGATGGA ACAACTCGAA AAACAAGATA AGACTGTCCG GAAACTGAAA 
AAACAACTTA AAGTATTTGC CAAAAAAATT GGTGAACTAG AAGTGGGCCA GATGGAGAAC 
GTATCACCAG GACAGATCAT TGATGAACCC ATTCGTCCAG TCAACATTCC CAGGAAGGAA 
AAGGATTTCC AAGGAATGCT GGAATACAAG AAGGAGGATG AGCAAAAACT TGTGAAGAAC 
CTGATTCTGG AGCTGAAGCC ACGTGGTGTG GCAGTCAATT TGATTCCAGG GTTACCAGCG 
TATATCTTGT TTATGTGTGT TCGGCATGCT GACTACCTGA ATGATGACCA GAAAGTAAGG 
TCGTTGCTAA CATCAACAAT TAACAGCATC AAGAAAGTAC TGAAGAAAAG AGGTGATGAT 
TTTGAAACCG TCTCCTTTTG GCTTTCCAAC ACATGCCGAT TTTTGCACTG TTTAAAGCAG 
TACAGTGGAG AAGAGGGCTT TATGAAACAC AACACCTCTC GCCAGAATGA ACACTGCCTC 
ACCAATTTTG ACTTGGCTGA GTACCGGCAG GTGCTCAGTG ACTTGGCAAT TCAGATCTAC 
CAGCAGCTCG TGCGGGTGTT AGAGAACATC CTTCAGCCGA TGATCGTCTC CGGAATGCTG 
GAGCATGAAA CCATTCAGGG TGTCTCTGGG GTGAAGCCCA CAGGGCTAAG AAAGCGAACC 
TCCAGCATCG CTGACGAGGG CACCTACACA CTGGACTCCA TCCTGCGGCA GCTCAACTCC 
TTCCACTCGG TCATGTGTCA GCACGGCATG GACCCCGAAC TGATCAAGCA GGTGGTCAAG 
CAGATGTTCT ACATCGTGGG GGCCGTCACC CTCAACAACC TCCTCTTGCG GAAGGACATG 
TGCTCCTGGA GTAAAGGCAT GCAGATCAGG TACAATGTCA GTCAACTAGA AGAATGGCTG 
CGTGACAAGA ATTTGATGAA CAGTGGGGCT AAGGAAACCC TGGAGCCTCT CATTCAGGCT 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACA GATGATGACG CAGAAGCCAT TTGTTCCATG 
TGCAATGCTT TAACTACTGC CCAGATTGTC AAAGTGTTGA ATTTGTATAC ACCGGTTAAT 
GAGTTTGAAG AAAGAGTTTC TGTGTCATTT ATTCGTACTA TACAGATGCG TTTACGAGAC 
AGGAAAGACT CTCCTCAACT ACTCATGGAT GCAAAACACA TCTTTCCTGT CACCTTTCCT 
TTTAACCCCT CTTCCCTTGC GCTAGAAACT ATCCAGATTC CAGCCAGCCT GGGCCTGGGA 
TTCATATCAC GCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYO5A
Entrez Gene ID	101105758
Full Name	myosin VA
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYO5A cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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