BRCA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRCA1
Entrez Gene ID	554178
Full Name	BRCA1, DNA repair associated
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016428866.1	XP_016284352.1	breast cancer type 1 susceptibility protein isoform X1
XM_007482129.2	XP_007482191.1	breast cancer type 1 susceptibility protein isoform X2
NM_001033969.1	NP_001029141.1	breast cancer type 1 susceptibility protein
XM_007482128.2	XP_007482190.1	breast cancer type 1 susceptibility protein isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comprehensive statistical study of 452 BRCA1 missense substitutions with classification of eight recurrent substitutions as neutral.
Tavtigian SV, Deffenbaugh AM, Yin L, Judkins T, Scholl T, Samollow PB, de Silva D, Zharkikh A, Thomas A
Journal of medical genetics43(4)295-305(2006 Apr)

Nuclear gene sequences provide evidence for the monophyly of australidelphian marsupials.
Amrine-Madsen H, Scally M, Westerman M, Stanhope MJ, Krajewski C, Springer MS
Molecular phylogenetics and evolution28(2)186-96(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc37110	NM_001033969.1 Latest version!	Monodelphis domestica BRCA1 DNA repair associated (BRCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc10263	XM_007482128.2 Latest version!	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc10263	XM_016428866.1 Latest version!	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc10264	XM_007482129.2 Latest version!	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRCA1 ( NM_001033969.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> NP_001029141.1 Monodelphis domestica BRCA1 DNA repair associated (BRCA1), mRNA.

Price & Availability↑

CloneID	OMc37110
Clone ID Related Accession (Same CDS sequence)	NM_001033969.1
Accession Version	NM_001033969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548259200000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	breast cancer type 1 susceptibility protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY994160.1. ##Evidence-Data-START## Transcript exon combination :: AY994160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2024560, SAMN00632153 [ECO:0000348] ##Evidence-Data-END##

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAGTAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACGTAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTACTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTA 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACGGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGGAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAAACT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029141.1
CDS	15..5537
Translation	MDLPTVTIEEVKNVLIGMQKILECPICLELIKEPVSTTCDHIFCRFCMLKLLSKKKGPSQCPLCKNNITKRSLRESTRFNQLVEGLLKTIRAFELDTGFQFSNTQDFSKWERRTPEPLKKEAATIQSIGYRNRSKRFKASESENSTLESSLGVQLYDLGIRKGSLRKQKKCIKNNAVYIKLGSDSSEDGVKNAICNSVKDQGLCQTSPKGTRLKSKEKAEYEFSERAIKSLQQHQSNTVDVHVINENATEGHSEESRGVSSSDLNMKPWNTDIHASSLPPEITSVLTNTVSMNIEKAELCDKSKRPGLARSQQISQDNSKEKCSAGKTSYAEVPHELNPHHLYERQELEEQPECPKYPRGNPQNCLSGTKLKSSIQKVNDWLSRSNDILVSDYSSVRIHEQNAEMASVLEIGHPDTTDGNSSISGKTDLVADSTDGAWLHMSERSCPRQAENNNIEDKIFGKTYHRKSVHTNLNYVTENLIVGAVASDCLIPPEHVKQTRLKRKRKTISDLQPEDFIKKTDTEFTHKSPEKKIHAVDQILEQEQNGQVMNTVNGHLEQKALVDGHVEEVNDALASELLPVEKESTFRTGTDSAAGSINHGGLKLNGRNAKMTKKDKLRKKSSARIVHELELIVDKNPSSSNETELQIDSYPSSEEIRKGNNSEQKQIRRSRRLQLLSEEIAMETKKAYEPDEQAEKSCVNEVFPDLKMGNIPACDTVSLTTESDQMLASCSVTEEGHEKSLEAVQSSQDQEDLAISGGEGSQGQRAKGNLEALEVPDTDWDTQDSTSLFPANTPQNSKAGPNPHRSQCGIMETPKELLDGCSSENTGSTTEDLRGLMRQGVKNASETTTEMEDSELDTQYLQNTFKRSKRQTFALGSSPRQECMKPCAISQALHQRGLHNATDCGDHEKEKLGNRESNKPVQAKSAVMNLAVVCQIERKPSDCASVSRICHIDPLHGGNDCEFIAGNNEEISQVPNQKQSVSPAGSSTSKIIYTKKLLEENLDEISPETAVGNEILAQSSLSLVSPSNSRDCVSKVADLNRFIGIGSNGEGSQAEKHKNKESELNTLPKLKLVQPQVCQQSFPQDNFSKEPEREEKEENVKLTPAISADSSPCLEQTKENTHFTQVWSETPDLLDSDGELKENTSFAESDIKEQSAVFGKNGKSSQVRKSRKNLSPLVHRNPSLSWKSRRQARKLESSEEEASSEEGELPSFQDLIFGKAASTPFQPTKNKTIAKEFSANEAKENLAFLNRNNMSVNNLQIPSGEASQDHDSSQESECSGSLFSSHSNSLEDLAGKANDKDSALVFGSSHQESSHSRTQEIQSGSEMPKASQDKEERETDLDEDHHPKDQGVDSNLVVEGSGYDSETSHPGDTSQLSFQSDILTTQQRDTMQDNLKKLQQEMAVLEAVLEQHSSQVTNNSSSQEPGLCPSMDQPDLKQTNSERGAEENVDHHKSQNVQSFSADKSRAFPSDSNSKNEEAGVEGSSFSKSQMPNRVWSPLSRSRTPWEGDSPSEESGKVTTRKEEHKRQPNEQGVLKQNLEITTEPESGVKVNLKNIKSNPHQNKDPALRNIFNLPTSTSALRSSQLQTIEATNTTAPCAPACNVRGQLKEDSASERLKKGGTGNRKISLVSSGLTPKENMLVQKFARKTHSTVSHQITEGTTHVIMKTDAEFVCERTLKYFLGIAGGKWVVSFLWVVQSFKEGKMLPECDFEVRGDVINGRNHRGPERARESQGMKIFRGLEICCYGPFTDMSTDQLEWMVQLCGASVVKKPSSLRFRVGSSPVVVVQPDAWEDDSSFQEIGLVCEAPVVTREWVLDSVACYQRQELDTYLISQTSPSLC

Target ORF information:

RefSeq Version	NM_001033969.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica BRCA1 DNA repair associated (BRCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033969.1

ORF Insert Sequence:

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAGTAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACGTAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTACTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTA 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACGGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGGAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAAACT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_016428866.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016284352.1 Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X2, mRNA.

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CloneID	OMc10263
Clone ID Related Accession (Same CDS sequence)	XM_016428866.1 , XM_007482128.2
Accession Version	XM_016428866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016284352.1
CDS	354..5876
Translation	MDLPTVTIEEVKNVLIGMQKILECPICLELIKEPVSTTCDHIFCRFCMLKLLSKKKGPSQCPLCKNNITKRSLRESTRFNQLVEGLLKTIRAFELDTGFQFSNTQDFSKWERRTPEPLKKEAATIQSIGYRNRSKRFKASESENSTLESSLGVQLYDLGIRKGSLRKQKKCIKNNAVYIKLGSDSSEDGVKNAICNSVKDQGLCQTSPKGTRLKSKEKAEYEFSERAIKSLQQHQSNTVDVHVINENATEGHSEESRGVSSSDLNMKPWNTDIHASSLPPEITSVLTNTVSMNIEKAELCDKSKRPGLARSQQISQDNNKEKCSAGKTSYAEVPHELNPHHLYERQELEEQPECPKYPRGNPQNCLSGTKLKSSIQKVNDWLSRSNDILVSDYSSVRIHEQNAEMASVLEIGHPDTTDGNSSISGKTDLVADSTDGAWLHMSERSCPRQAENNNIEDKIFGKTYHRKSVHTNLNYITENLIVGAVASDCLIPPEHVKQTRLKRKRKTISDLQPEDFIKKTDTEFIHKSPEKKIHAVDQILEQEQNGQVMNTVNGHLEQKALVDGHVEEVNDALASELLPVEKESTFRTGTDSAAGSINHRGLKLNGRNAKMTKKDKLRKKSSARIVHELELIVDKNPSSSNETELQIDSYPSSEEIRKGNNSEQKQIRRSRRLQLLSEEIAMETKKAYEPDEQAEKSCVNEVFPDLKMGNIPACDTVSLTTESDQMLASCSVTEEGHEKSLEAVQSSQDQEDLAISGGEGSQGQRAKGNLEALEVPDTDWDTQDSTSLFPANTPQNSKAGPNPHRSQCEIMETPKELLDGCSSENTGSTTEDLRGLMRQGVKNASETTTEMEDSELDTQYLQNTFKRSKRQTFALGSSPRQECMKPCAISQALHQRGLHNATDCGDHEKEKLGNRESNKPVQAKSAVMNLAVVCQIERKPSDCASVSRICHIDPLHGGNDCEFIAGNNEEISQVPNQKQSVSPAGSSTSKIIYTKNLLEENLDEISPETAVGNEILAQSSLSLVSPSNSRDCVSKVADLNRFIGIGSNGEGSQAEKHKNKESELNTLPKLKLVQPQVCQQSFPQDNFSKEPEREEKEENVKLTPAISADSSPCLEQTKENTHFTQVWSETPDLLDSDGELKENTSFAESDIKEQSAVFGKNGKSSQVRKSRKNLSPLVHRNPSLSWKSRRQARKLESSEEEASSEEGELPSFQDLIFGKAASTPFQPTKNKTIAKEFSANEAKENLAFLNRNNMSVNNLQIPSGEASQDHDSSQESECSGSLFSSHSNSLEDLAGKANDKDSALVFGSSHQESSHSRTQEIQSGSEMPKASQDKEERETDLDEDHHPKDQGVDSNLVVEGSGYDSETSHPGDTSQLSFQSDILTTQQRDTMQDNLKKLQQEMAVLEAVLEQHSSQVTNNSSSQEPGLCPSMDQPDLKQTNSERGAEENVDHHKSQNVQSFSADKSRAFPSDSNSKNEEAGVEGSSFSKSQMPNRVWSPLSRSRTPWEGDSPSEESGKVTTRKEEHKRQPNEQGVLKQNLEITTEPESGVKVNLKNIKSNPHQNKDPALRNIFNLPTSTSALRSSQLQTIEATNTTAPCAPACNVRGQLKEDSASERLKKGGTGNRKISLVSSGLTPKENMLVQKFARKTHSTVSHQITEGTTHVIMKTDAEFVCERTLKYFLGIAGGKWVVSFLWVVQSFKEGKMLPECDFEVRGDVINGRNHRGPERARESQGMKIFRGLEICCYGPFTDMSTDQLEWMVQLCGASVVKKPSSLRFRVGSSPVVVVQPDAWEDDSSFQEIGLVCEAPVVTREWVLDSVACYQRQELDTYLISQTSPSLC

Target ORF information:

RefSeq Version	XM_016428866.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016428866.1

ORF Insert Sequence:

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_007482128.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007482190.1 Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X1, mRNA.

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CloneID	OMc10263
Clone ID Related Accession (Same CDS sequence)	XM_016428866.1 , XM_007482128.2
Accession Version	XM_007482128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007482128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007482190.1
CDS	360..5882
Translation	MDLPTVTIEEVKNVLIGMQKILECPICLELIKEPVSTTCDHIFCRFCMLKLLSKKKGPSQCPLCKNNITKRSLRESTRFNQLVEGLLKTIRAFELDTGFQFSNTQDFSKWERRTPEPLKKEAATIQSIGYRNRSKRFKASESENSTLESSLGVQLYDLGIRKGSLRKQKKCIKNNAVYIKLGSDSSEDGVKNAICNSVKDQGLCQTSPKGTRLKSKEKAEYEFSERAIKSLQQHQSNTVDVHVINENATEGHSEESRGVSSSDLNMKPWNTDIHASSLPPEITSVLTNTVSMNIEKAELCDKSKRPGLARSQQISQDNNKEKCSAGKTSYAEVPHELNPHHLYERQELEEQPECPKYPRGNPQNCLSGTKLKSSIQKVNDWLSRSNDILVSDYSSVRIHEQNAEMASVLEIGHPDTTDGNSSISGKTDLVADSTDGAWLHMSERSCPRQAENNNIEDKIFGKTYHRKSVHTNLNYITENLIVGAVASDCLIPPEHVKQTRLKRKRKTISDLQPEDFIKKTDTEFIHKSPEKKIHAVDQILEQEQNGQVMNTVNGHLEQKALVDGHVEEVNDALASELLPVEKESTFRTGTDSAAGSINHRGLKLNGRNAKMTKKDKLRKKSSARIVHELELIVDKNPSSSNETELQIDSYPSSEEIRKGNNSEQKQIRRSRRLQLLSEEIAMETKKAYEPDEQAEKSCVNEVFPDLKMGNIPACDTVSLTTESDQMLASCSVTEEGHEKSLEAVQSSQDQEDLAISGGEGSQGQRAKGNLEALEVPDTDWDTQDSTSLFPANTPQNSKAGPNPHRSQCEIMETPKELLDGCSSENTGSTTEDLRGLMRQGVKNASETTTEMEDSELDTQYLQNTFKRSKRQTFALGSSPRQECMKPCAISQALHQRGLHNATDCGDHEKEKLGNRESNKPVQAKSAVMNLAVVCQIERKPSDCASVSRICHIDPLHGGNDCEFIAGNNEEISQVPNQKQSVSPAGSSTSKIIYTKNLLEENLDEISPETAVGNEILAQSSLSLVSPSNSRDCVSKVADLNRFIGIGSNGEGSQAEKHKNKESELNTLPKLKLVQPQVCQQSFPQDNFSKEPEREEKEENVKLTPAISADSSPCLEQTKENTHFTQVWSETPDLLDSDGELKENTSFAESDIKEQSAVFGKNGKSSQVRKSRKNLSPLVHRNPSLSWKSRRQARKLESSEEEASSEEGELPSFQDLIFGKAASTPFQPTKNKTIAKEFSANEAKENLAFLNRNNMSVNNLQIPSGEASQDHDSSQESECSGSLFSSHSNSLEDLAGKANDKDSALVFGSSHQESSHSRTQEIQSGSEMPKASQDKEERETDLDEDHHPKDQGVDSNLVVEGSGYDSETSHPGDTSQLSFQSDILTTQQRDTMQDNLKKLQQEMAVLEAVLEQHSSQVTNNSSSQEPGLCPSMDQPDLKQTNSERGAEENVDHHKSQNVQSFSADKSRAFPSDSNSKNEEAGVEGSSFSKSQMPNRVWSPLSRSRTPWEGDSPSEESGKVTTRKEEHKRQPNEQGVLKQNLEITTEPESGVKVNLKNIKSNPHQNKDPALRNIFNLPTSTSALRSSQLQTIEATNTTAPCAPACNVRGQLKEDSASERLKKGGTGNRKISLVSSGLTPKENMLVQKFARKTHSTVSHQITEGTTHVIMKTDAEFVCERTLKYFLGIAGGKWVVSFLWVVQSFKEGKMLPECDFEVRGDVINGRNHRGPERARESQGMKIFRGLEICCYGPFTDMSTDQLEWMVQLCGASVVKKPSSLRFRVGSSPVVVVQPDAWEDDSSFQEIGLVCEAPVVTREWVLDSVACYQRQELDTYLISQTSPSLC

Target ORF information:

RefSeq Version	XM_007482128.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007482128.2

ORF Insert Sequence:

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG GAGCAGAGGA AAATGTTGAC CATCACAAAA GTCAAAATGT GCAAAGCTTT 
TCAGCAGACA AGTCCCGAGC ATTTCCAAGT GATTCCAACA GCAAAAATGA AGAAGCAGGA 
GTTGAAGGGT CTTCTTTTTC CAAGTCCCAA ATGCCGAATA GGGTTTGGAG CCCACTCAGT 
CGCTCCAGGA CTCCTTGGGA AGGAGACTCC CCTTCTGAAG AGAGTGGTAA AGTGACCACC 
AGAAAGGAAG AACACAAGCG GCAACCCAAC GAACAGGGTG TGCTGAAACA AAACCTAGAA 
ATAACCACTG AGCCAGAATC TGGAGTCAAA GTAAACCTTA AGAACATCAA ATCCAATCCA 
CATCAAAATA AGGACCCTGC CCTAAGAAAT ATCTTCAATC TGCCCACCTC GACTTCTGCA 
CTGAGGTCAT CCCAGCTTCA GACCATAGAA GCCACCAACA CCACTGCTCC TTGTGCTCCT 
GCTTGCAATG TGAGGGGCCA ACTGAAGGAG GATTCTGCCT CAGAGAGGCT TAAAAAAGGA 
GGGACAGGAA ACCGAAAGAT ATCCCTTGTG TCATCGGGTT TAACTCCTAA AGAAAATATG 
CTAGTGCAGA AGTTTGCCAG GAAAACCCAC AGCACTGTAT CTCATCAAAT TACTGAAGGG 
ACCACTCATG TCATAATGAA AACAGATGCT GAGTTTGTGT GTGAGCGGAC CCTGAAGTAC 
TTCTTGGGCA TTGCAGGAGG GAAATGGGTA GTCAGTTTTC TGTGGGTGGT GCAGTCTTTT 
AAAGAAGGAA AGATGCTGCC TGAGTGTGAC TTTGAAGTCA GAGGAGATGT CATCAATGGG 
AGAAACCACC GAGGCCCAGA AAGAGCAAGA GAATCTCAGG GGATGAAGAT CTTCAGGGGC 
CTGGAAATCT GTTGCTATGG ACCATTTACA GACATGTCAA CAGATCAGCT GGAGTGGATG 
GTGCAACTGT GTGGGGCCTC GGTAGTGAAG AAACCTTCCT CTTTGAGGTT CAGGGTGGGT 
TCTTCCCCGG TTGTGGTGGT GCAACCTGAT GCTTGGGAAG ATGACAGCAG TTTTCAAGAA 
ATCGGGCTGG TATGTGAAGC ACCTGTAGTG ACCCGGGAGT GGGTGCTGGA CAGCGTTGCC 
TGTTATCAGC GCCAGGAGCT GGACACCTAC TTGATATCCC AGACCTCCCC AAGCCTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_007482129.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007482191.1 Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X3, mRNA.

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CloneID	OMc10264
Clone ID Related Accession (Same CDS sequence)	XM_007482129.2
Accession Version	XM_007482129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007482129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG AGGAAAATGT TGACCATCAC AAAAGTCAAA ATGTGCAAAG CTTTTCAGCA 
GACAAGTCCC GAGCATTTCC AAGTGATTCC AACAGCAAAA ATGAAGAAGC AGGAGTTGAA 
GGGTCTTCTT TTTCCAAGTC CCAAATGCCG AATAGGGTTT GGAGCCCACT CAGTCGCTCC 
AGGACTCCTT GGGAAGGAGA CTCCCCTTCT GAAGAGAGTG GTAAAGTGAC CACCAGAAAG 
GAAGAACACA AGCGGCAACC CAACGAACAG GGTGTGCTGA AACAAAACCT AGAAATAACC 
ACTGAGCCAG AATCTGGAGT CAAAGTAAAC CTTAAGAACA TCAAATCCAA TCCACATCAA 
AATAAGGACC CTGCCCTAAG AAATATCTTC AATCTGCCCA CCTCGACTTC TGCACTGAGG 
TCATCCCAGC TTCAGACCAT AGAAGCCACC AACACCACTG CTCCTTGTGC TCCTGCTTGC 
AATGTGAGGG GCCAACTGAA GGAGGATTCT GCCTCAGAGA GGCTTAAAAA AGGAGGGACA 
GGAAACCGAA AGATATCCCT TGTGTCATCG GGTTTAACTC CTAAAGAAAA TATGCTAGTG 
CAGAAGTTTG CCAGGAAAAC CCACAGCACT GTATCTCATC AAATTACTGA AGGGACCACT 
CATGTCATAA TGAAAACAGA TGCTGAGTTT GTGTGTGAGC GGACCCTGAA GTACTTCTTG 
GGCATTGCAG GAGGGAAATG GGTAGTCAGT TTTCTGTGGG TGGTGCAGTC TTTTAAAGAA 
GGAAAGATGC TGCCTGAGTG TGACTTTGAA GTCAGAGGAG ATGTCATCAA TGGGAGAAAC 
CACCGAGGCC CAGAAAGAGC AAGAGAATCT CAGGGGATGA AGATCTTCAG GGGCCTGGAA 
ATCTGTTGCT ATGGACCATT TACAGACATG TCAACAGATC AGCTGGAGTG GATGGTGCAA 
CTGTGTGGGG CCTCGGTAGT GAAGAAACCT TCCTCTTTGA GGTTCAGGGT GGGTTCTTCC 
CCGGTTGTGG TGGTGCAACC TGATGCTTGG GAAGATGACA GCAGTTTTCA AGAAATCGGG 
CTGGTATGTG AAGCACCTGT AGTGACCCGG GAGTGGGTGC TGGACAGCGT TGCCTGTTAT 
CAGCGCCAGG AGCTGGACAC CTACTTGATA TCCCAGACCT CCCCAAGCCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007482191.1
CDS	360..5876
Translation	MDLPTVTIEEVKNVLIGMQKILECPICLELIKEPVSTTCDHIFCRFCMLKLLSKKKGPSQCPLCKNNITKRSLRESTRFNQLVEGLLKTIRAFELDTGFQFSNTQDFSKWERRTPEPLKKEAATIQSIGYRNRSKRFKASESENSTLESSLGVQLYDLGIRKGSLRKQKKCIKNNAVYIKLGSDSSEDGVKNAICNSVKDQGLCQTSPKGTRLKSKEKAEYEFSERAIKSLQQHQSNTVDVHVINENATEGHSEESRGVSSSDLNMKPWNTDIHASSLPPEITSVLTNTVSMNIEKAELCDKSKRPGLARSQQISQDNNKEKCSAGKTSYAEVPHELNPHHLYERQELEEQPECPKYPRGNPQNCLSGTKLKSSIQKVNDWLSRSNDILVSDYSSVRIHEQNAEMASVLEIGHPDTTDGNSSISGKTDLVADSTDGAWLHMSERSCPRQAENNNIEDKIFGKTYHRKSVHTNLNYITENLIVGAVASDCLIPPEHVKQTRLKRKRKTISDLQPEDFIKKTDTEFIHKSPEKKIHAVDQILEQEQNGQVMNTVNGHLEQKALVDGHVEEVNDALASELLPVEKESTFRTGTDSAAGSINHRGLKLNGRNAKMTKKDKLRKKSSARIVHELELIVDKNPSSSNETELQIDSYPSSEEIRKGNNSEQKQIRRSRRLQLLSEEIAMETKKAYEPDEQAEKSCVNEVFPDLKMGNIPACDTVSLTTESDQMLASCSVTEEGHEKSLEAVQSSQDQEDLAISGGEGSQGQRAKGNLEALEVPDTDWDTQDSTSLFPANTPQNSKAGPNPHRSQCEIMETPKELLDGCSSENTGSTTEDLRGLMRQGVKNASETTTEMEDSELDTQYLQNTFKRSKRQTFALGSSPRQECMKPCAISQALHQRGLHNATDCGDHEKEKLGNRESNKPVQAKSAVMNLAVVCQIERKPSDCASVSRICHIDPLHGGNDCEFIAGNNEEISQVPNQKQSVSPAGSSTSKIIYTKNLLEENLDEISPETAVGNEILAQSSLSLVSPSNSRDCVSKVADLNRFIGIGSNGEGSQAEKHKNKESELNTLPKLKLVQPQVCQQSFPQDNFSKEPEREEKEENVKLTPAISADSSPCLEQTKENTHFTQVWSETPDLLDSDGELKENTSFAESDIKEQSAVFGKNGKSSQVRKSRKNLSPLVHRNPSLSWKSRRQARKLESSEEEASSEEGELPSFQDLIFGKAASTPFQPTKNKTIAKEFSANEAKENLAFLNRNNMSVNNLQIPSGEASQDHDSSQESECSGSLFSSHSNSLEDLAGKANDKDSALVFGSSHQESSHSRTQEIQSGSEMPKASQDKEERETDLDEDHHPKDQGVDSNLVVEGSGYDSETSHPGDTSQLSFQSDILTTQQRDTMQDNLKKLQQEMAVLEAVLEQHSSQVTNNSSSQEPGLCPSMDQPDLKQTNSEREENVDHHKSQNVQSFSADKSRAFPSDSNSKNEEAGVEGSSFSKSQMPNRVWSPLSRSRTPWEGDSPSEESGKVTTRKEEHKRQPNEQGVLKQNLEITTEPESGVKVNLKNIKSNPHQNKDPALRNIFNLPTSTSALRSSQLQTIEATNTTAPCAPACNVRGQLKEDSASERLKKGGTGNRKISLVSSGLTPKENMLVQKFARKTHSTVSHQITEGTTHVIMKTDAEFVCERTLKYFLGIAGGKWVVSFLWVVQSFKEGKMLPECDFEVRGDVINGRNHRGPERARESQGMKIFRGLEICCYGPFTDMSTDQLEWMVQLCGASVVKKPSSLRFRVGSSPVVVVQPDAWEDDSSFQEIGLVCEAPVVTREWVLDSVACYQRQELDTYLISQTSPSLC

Target ORF information:

RefSeq Version	XM_007482129.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica breast cancer 1 (BRCA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007482129.2

ORF Insert Sequence:

ATGGATTTGC CTACAGTCAC TATTGAAGAA GTAAAGAATG TCCTAATTGG GATGCAGAAA 
ATTTTAGAAT GTCCTATCTG TCTGGAGTTG ATCAAAGAGC CTGTTTCTAC TACCTGTGAC 
CACATATTTT GCAGATTTTG TATGTTGAAG CTTCTCAGCA AGAAGAAGGG GCCCTCTCAA 
TGTCCACTGT GCAAGAACAA TATAACCAAA AGAAGCCTTC GAGAAAGTAC AAGATTTAAT 
CAGCTGGTGG AGGGACTGCT GAAAACCATT CGTGCTTTTG AACTTGACAC AGGATTCCAA 
TTTTCAAATA CTCAAGATTT TTCAAAATGG GAGAGGAGGA CTCCTGAGCC TCTAAAGAAG 
GAAGCTGCTA CCATTCAAAG TATAGGCTAT AGAAACCGCT CAAAAAGATT CAAAGCCAGT 
GAATCAGAAA ATTCAACCTT GGAATCCAGT CTTGGCGTCC AGCTCTATGA CCTTGGCATC 
CGAAAAGGTT CCCTGAGAAA GCAAAAGAAA TGCATTAAAA ATAATGCTGT CTACATCAAG 
TTAGGATCTG ATTCTTCTGA AGATGGTGTG AAAAATGCAA TCTGTAACAG TGTCAAAGAC 
CAAGGATTGT GCCAAACCTC ACCAAAAGGA ACCAGATTAA AGTCCAAGGA AAAGGCTGAA 
TATGAGTTTT CTGAGAGGGC CATAAAGAGC CTACAACAAC ATCAATCTAA CACTGTTGAT 
GTGCATGTCA TCAATGAGAA TGCCACTGAA GGGCATTCAG AGGAGTCTCG GGGTGTTTCT 
TCCTCAGACT TGAATATGAA GCCATGGAAC ACAGACATTC ATGCTAGCTC ATTACCACCT 
GAGATCACCA GTGTATTGAC TAACACAGTC AGCATGAACA TAGAAAAGGC TGAACTCTGT 
GATAAAAGTA AAAGGCCTGG TTTAGCAAGG AGCCAGCAGA TAAGTCAGGA TAATAATAAG 
GAAAAATGTA GTGCTGGGAA GACCTCATAT GCAGAGGTGC CGCATGAGCT GAACCCCCAT 
CATCTGTATG AGAGGCAAGA ACTAGAGGAA CAGCCAGAGT GCCCCAAGTA CCCCAGAGGA 
AATCCTCAAA ACTGCTTGTC TGGAACCAAA CTGAAAAGCA GTATTCAGAA AGTTAATGAC 
TGGTTATCCA GAAGTAATGA CATTTTAGTC TCTGATTATT CCTCTGTTAG GATCCATGAA 
CAGAATGCAG AGATGGCTAG TGTCTTAGAA ATTGGGCATC CAGATACCAC AGATGGAAAT 
TCTAGCATTT CTGGGAAGAC TGACTTGGTG GCTGACTCCA CTGATGGTGC CTGGCTACAT 
ATGTCTGAAA GAAGCTGCCC CAGGCAGGCA GAGAACAACA ATATTGAAGA CAAAATATTT 
GGAAAAACCT ACCATAGAAA GTCAGTTCAC ACTAATTTGA ATTACATAAC TGAAAACTTG 
ATTGTTGGAG CTGTTGCTTC TGATTGTTTG ATCCCTCCAG AGCATGTCAA ACAAACCAGG 
TTAAAGCGTA AAAGGAAGAC CATCTCTGAC CTACAGCCTG AGGATTTCAT CAAGAAGACA 
GACACTGAAT TTATTCACAA ATCCCCTGAA AAGAAAATTC ATGCTGTTGA CCAAATTCTC 
GAACAAGAAC AAAATGGCCA AGTGATGAAC ACAGTTAATG GCCATCTCGA ACAGAAAGCA 
TTAGTAGATG GTCATGTAGA GGAAGTGAAT GATGCTCTGG CATCAGAACT GCTCCCTGTG 
GAGAAAGAAT CCACTTTCAG AACTGGAACA GACTCCGCAG CTGGCAGCAT AAACCACAGG 
GGACTCAAAT TAAATGGTAG AAATGCCAAA ATGACAAAAA AGGACAAGCT GAGGAAGAAG 
TCTTCAGCCA GGATTGTCCA TGAGCTTGAG CTCATAGTTG ATAAAAACCC AAGCTCTTCT 
AATGAAACTG AACTACAGAT CGATAGCTAT CCCAGCAGTG AAGAGATAAG GAAGGGAAAT 
AATTCTGAAC AGAAGCAAAT CAGACGCAGC AGAAGGCTTC AGCTGTTGTC AGAAGAAATT 
GCAATGGAAA CCAAGAAGGC CTATGAGCCA GATGAACAAG CAGAAAAAAG TTGTGTCAAT 
GAAGTTTTCC CAGATCTAAA AATGGGAAAT ATACCTGCCT GTGATACTGT CAGTCTCACT 
ACTGAGAGTG ATCAAATGTT AGCTAGCTGC AGTGTCACTG AAGAAGGACA TGAAAAGAGC 
CTGGAAGCAG TCCAGAGCAG CCAAGATCAA GAGGATCTGG CAATAAGTGG AGGGGAGGGA 
TCACAAGGCC AGAGAGCCAA AGGAAACCTG GAGGCCTTGG AGGTTCCTGA TACTGATTGG 
GACACTCAGG ACAGTACCTC ATTGTTTCCA GCTAATACTC CGCAAAATTC AAAAGCAGGA 
CCCAATCCTC ACAGAAGTCA ATGTGAAATA ATGGAAACCC CCAAGGAACT CTTAGATGGT 
TGTTCATCTG AAAACACTGG AAGCACCACA GAAGATTTGA GGGGCCTGAT GAGACAGGGA 
GTTAAAAATG CCTCAGAGAC AACCACAGAA ATGGAGGATA GTGAACTCGA CACCCAGTAT 
TTACAGAATA CCTTCAAACG TTCAAAACGC CAGACATTTG CTCTGGGTTC CAGTCCAAGG 
CAGGAATGTA TGAAACCCTG TGCTATTTCT CAGGCTTTAC ATCAGCGGGG TCTACATAAT 
GCCACAGATT GTGGGGACCA TGAAAAAGAA AAGCTGGGAA ACAGAGAATC CAACAAGCCC 
GTGCAAGCAA AGTCTGCAGT CATGAACTTA GCTGTAGTTT GTCAGATAGA GAGGAAGCCA 
AGTGATTGTG CTAGTGTCTC CAGGATTTGT CACATAGATC CATTGCATGG TGGCAATGAC 
TGTGAATTCA TTGCTGGAAA TAACGAGGAA ATTTCACAAG TTCCTAATCA AAAGCAATCA 
GTTTCTCCAG CAGGATCATC TACTAGTAAA ATTATATATA CAAAAAACCT CTTGGAGGAA 
AACCTTGATG AAATATCCCC TGAAACAGCT GTGGGAAATG AAATCTTAGC CCAAAGTAGC 
TTAAGTCTAG TTAGCCCAAG TAACAGCAGA GACTGTGTCT CTAAAGTAGC TGACTTAAAC 
AGATTTATTG GCATAGGGTC TAATGGAGAA GGCAGTCAAG CAGAAAAACA TAAAAACAAA 
GAATCTGAAT TAAATACACT ACCTAAATTA AAGCTTGTGC AACCTCAAGT TTGTCAACAA 
AGCTTTCCTC AGGATAACTT TAGCAAGGAA CCTGAAAGAG AAGAGAAAGA AGAAAATGTA 
AAATTAACTC CGGCTATTAG TGCAGATTCA TCTCCATGTT TAGAACAAAC TAAAGAGAAT 
ACACATTTTA CTCAGGTTTG GTCTGAGACT CCTGACTTAT TGGACAGTGA TGGTGAACTT 
AAAGAAAACA CCAGCTTTGC TGAAAGTGAT ATCAAAGAAC AATCTGCTGT TTTTGGCAAA 
AATGGTAAAA GTTCTCAAGT AAGAAAATCT AGAAAAAACC TCAGCCCTTT AGTACATAGA 
AATCCCAGTT TGAGTTGGAA ATCCCGAAGG CAAGCCCGGA AACTAGAGTC CTCAGAAGAA 
GAAGCCTCCA GTGAGGAGGG AGAGCTTCCA AGTTTTCAAG ACTTAATATT TGGTAAAGCA 
GCAAGTACAC CCTTTCAGCC TACCAAAAAT AAAACCATTG CCAAAGAGTT TTCAGCAAAT 
GAGGCAAAAG AGAATCTAGC CTTCCTAAAC AGAAATAATA TGAGTGTTAA CAATCTTCAG 
ATACCATCTG GGGAGGCATC CCAAGATCAT GATTCCAGCC AAGAATCGGA ATGTTCTGGT 
AGCTTATTTT CTTCGCACTC TAATTCATTA GAAGACTTAG CTGGGAAAGC AAATGACAAG 
GATTCTGCTT TGGTGTTTGG CTCTTCCCAT CAAGAATCAA GTCATTCCCG GACCCAAGAA 
ATCCAGAGTG GGAGTGAAAT GCCCAAGGCT TCCCAAGATA AGGAAGAAAG GGAAACTGAC 
CTAGATGAAG ACCATCACCC CAAGGACCAG GGTGTGGATT CAAATTTAGT TGTTGAAGGA 
TCTGGGTATG ACAGTGAAAC AAGCCATCCT GGAGACACTT CACAGCTCTC GTTTCAGAGT 
GATATTTTAA CCACTCAGCA AAGGGATACC ATGCAAGACA ACCTGAAAAA ACTCCAACAG 
GAAATGGCTG TCCTAGAGGC TGTTTTGGAG CAACATAGTA GTCAGGTCAC CAACAACTCC 
AGTTCTCAGG AACCTGGGCT ATGCCCCTCC ATGGACCAGC CAGACTTGAA ACAGACCAAT 
TCAGAAAGAG AGGAAAATGT TGACCATCAC AAAAGTCAAA ATGTGCAAAG CTTTTCAGCA 
GACAAGTCCC GAGCATTTCC AAGTGATTCC AACAGCAAAA ATGAAGAAGC AGGAGTTGAA 
GGGTCTTCTT TTTCCAAGTC CCAAATGCCG AATAGGGTTT GGAGCCCACT CAGTCGCTCC 
AGGACTCCTT GGGAAGGAGA CTCCCCTTCT GAAGAGAGTG GTAAAGTGAC CACCAGAAAG 
GAAGAACACA AGCGGCAACC CAACGAACAG GGTGTGCTGA AACAAAACCT AGAAATAACC 
ACTGAGCCAG AATCTGGAGT CAAAGTAAAC CTTAAGAACA TCAAATCCAA TCCACATCAA 
AATAAGGACC CTGCCCTAAG AAATATCTTC AATCTGCCCA CCTCGACTTC TGCACTGAGG 
TCATCCCAGC TTCAGACCAT AGAAGCCACC AACACCACTG CTCCTTGTGC TCCTGCTTGC 
AATGTGAGGG GCCAACTGAA GGAGGATTCT GCCTCAGAGA GGCTTAAAAA AGGAGGGACA 
GGAAACCGAA AGATATCCCT TGTGTCATCG GGTTTAACTC CTAAAGAAAA TATGCTAGTG 
CAGAAGTTTG CCAGGAAAAC CCACAGCACT GTATCTCATC AAATTACTGA AGGGACCACT 
CATGTCATAA TGAAAACAGA TGCTGAGTTT GTGTGTGAGC GGACCCTGAA GTACTTCTTG 
GGCATTGCAG GAGGGAAATG GGTAGTCAGT TTTCTGTGGG TGGTGCAGTC TTTTAAAGAA 
GGAAAGATGC TGCCTGAGTG TGACTTTGAA GTCAGAGGAG ATGTCATCAA TGGGAGAAAC 
CACCGAGGCC CAGAAAGAGC AAGAGAATCT CAGGGGATGA AGATCTTCAG GGGCCTGGAA 
ATCTGTTGCT ATGGACCATT TACAGACATG TCAACAGATC AGCTGGAGTG GATGGTGCAA 
CTGTGTGGGG CCTCGGTAGT GAAGAAACCT TCCTCTTTGA GGTTCAGGGT GGGTTCTTCC 
CCGGTTGTGG TGGTGCAACC TGATGCTTGG GAAGATGACA GCAGTTTTCA AGAAATCGGG 
CTGGTATGTG AAGCACCTGT AGTGACCCGG GAGTGGGTGC TGGACAGCGT TGCCTGTTAT 
CAGCGCCAGG AGCTGGACAC CTACTTGATA TCCCAGACCT CCCCAAGCCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comprehensive statistical study of 452 BRCA1 missense substitutions with classification of eight recurrent substitutions as neutral.
Journal of medical genetics43(4)295-305(2006 Apr)
Tavtigian SV,Deffenbaugh AM,Yin L,Judkins T,Scholl T,Samollow PB,de Silva D,Zharkikh A,Thomas A

Nuclear gene sequences provide evidence for the monophyly of australidelphian marsupials.
Molecular phylogenetics and evolution28(2)186-96(2003 Aug)
Amrine-Madsen H,Scally M,Westerman M,Stanhope MJ,Krajewski C,Springer MS

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BRCA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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