CFAP54 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFAP54
Entrez Gene ID	103106524
Full Name	cilia and flagella associated protein 54
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016425734.1	XP_016281220.1	cilia- and flagella-associated protein 54 isoform X2
XM_016425736.1	XP_016281222.1	cilia- and flagella-associated protein 54 isoform X4
XM_016425737.1	XP_016281223.1	cilia- and flagella-associated protein 54 isoform X6
XM_007503244.2	XP_007503306.1	cilia- and flagella-associated protein 54 isoform X1
XM_007503246.2	XP_007503308.1	cilia- and flagella-associated protein 54 isoform X5
XM_016425735.1	XP_016281221.1	cilia- and flagella-associated protein 54 isoform X3

Pathways from BioSystems
Gene Ontology

The following CFAP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFAP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc32357	XM_007503244.2 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc32359	XM_007503246.2 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc45043	XM_016425734.1 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc45044	XM_016425735.1 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc45045	XM_016425736.1 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc45046	XM_016425737.1 Latest version!	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFAP54 ( XM_007503244.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007503306.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc32357
Clone ID Related Accession (Same CDS sequence)	XM_007503244.2
Accession Version	XM_007503244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007503244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCC TGAAAGTCCC CGGCTTCGTG TCCCCAAGCG GCTCAGCCTC AGGCTCTGTC 
AGCTCTATGA CGTCGGTGTC GGACTACTCC TGGAGCCAGT CCTCGGAGGG GGACAAGGGC 
GATACGGAGG TGACGCTCCA AGTCCCCTCG TCCCGCAGCT TCTTGACCCG GCTCCCCAAG 
ACCCGAGGGT CTCCCTTTCG AGGACTCGGG CAACCCTTGA CGGCGGCCCC CGCGACTACG 
GCTTCCCGCC GGCGCCGCCG TGATCCCTTC TTGGAGTCAA TAGCGATGAT GAACGTCATG 
AGGCAGATGC CAATCAAGGC GCTTCGCAAG TTTCCGGTCA TGGAGAGGAA GAGCTCTGAG 
TGCCGCTGCC TACTCCGGCA ACGACCTCGT CCTTGCAACC TTATTGAGCA GCCCAGAGCT 
GTCTTCTATG GGCCCCTGGA CGCCAAAAAC CCGCTGCTTA TCTCCTTCCA GAAGGAGCTG 
AAGGGCGTGC TGGACTTCCT CAAGAGGAAG CAGAGTCAGA CCCACACTCC CGAGGAATAC 
CAGGAGTACC AGCAACGCGG TACCTTCACC TTGTTCAATA TCTGGTTCAA GTATCAGAAC 
CGCCTGCCAG TCCAGTATTA CCATGAGCAT CTTTTGAAGA CTGGAGACAC CCTTATTCAA 
GCAAAAGAAT ACAAGTTGGC CTTAATACAA TGCTATGGGA AGTATCTTAG TCAGTTCAAT 
CCCAGTCACG ATGAGAGTGA CATGAACGTG ACAACTTTCA AAAAAGTCTT TTTCTCCAAA 
GGTGTCAATG ATGAAAGCGC CAAACTTACA TTTCACGCTT TGCAAGGGAA ATGTATTAGC 
CAGTACCAAA TTATCTGTGC CACCGATCCC TGTTTCAAGA GGGAGCTGTC TGTGGAGCAG 
TGCTTCAAGA TTCTGTCGGT TTTTCGGCTC ATGATGCAGA TAACCCTGCC CTACGAACAC 
CTTTGCTGGA TCGTGTACAA TGGTACAATT CACATTTATA CAATTTGCAG GAAGCTGATG 
ACCATTGGAC AGTCTGCTAA GGCCTTGGAA TTCTTGCTCT GGGCCAGTAT TTGTATGGAA 
TCCTCTTTAC CCCTCTTAGC AGTAAGCTAT CTCAGTTGGA GAGCTACCCT GTATACTGCT 
GTTTGCCAGT GCTACTATGA CTGCCATGCT GGAATGCAGG GAGAGATATT TGCTCGGCGA 
GGTTTGGGAA AAATTGATGA TTTATTACGT CTAGAAGTAA TGAGTATCAC ACCACCACAG 
GAGGAAGAAA GAAAAAAATT CCGAGAGGCC ACCATCAAGA TGGCGGTGAT GATCTTTAAG 
AGAGGAGTCT TTGAACCACG AAGAAAGAGT AAGTCTTATG TAAGAACAAA ACTGAGACTG 
AACCTACGGG AAGCACAGAA TTTGCAATGG CCACGCACAG CTACAGAGAA GTTGTTGAGT 
GAGATGTTTG ATTGTGGATC AGCCCAGTTT TTGGCTGTCT TGGAAGCCCT TTCTGAACCA 
AATAGAAGAG TGTTACAAAC TGGTCACTTG TCAGAGGAAA TGGAAGTTCG TGAGGTCATC 
GCTGAGCTCT TCATGGCAGC AAGCGAGCTT TTATCAGATG CATGTAGTAG CCATGGGAAA 
TCCAGTTTTT CCAAAAACAT CTCTCTACAG CTTTCTCAAA TGCAACTCGC CATTGAAAGC 
AAACAAAGTG TTTCAATAGC TGCTGCAGTG AAGTTGATAA AATTATCTTT CACCTTTGAG 
GAATGGCATT TGTTTAATTC TGCTGCTTCT CAGTTTATCG GTTTTCTTCA GCTTCAAAGT 
GGCCCACACT TGCAGAAAGC CAGGAAGGAT TTAATGCTTC TGGCTGCGAT GGAACCGCTA 
ATTAATGTGA AGCGGAATAA AGGCTTAAGC TATGCTGGTG AAGGAGGCAG CACTACCACT 
GAAGGAGCTC GAAGCTCAGG GAGAAAAATT GTGTTTCAAG AAAATTTCTC ATTATCTGGA 
AGCTATTCTG ATGATATTTT CTATTTGGCA TCGATGCTGT TTTCCTTCAT TTGTTCACCT 
ACTGAGAAAA TTCGTCCTGA TGAAGAGATC GTTGTTGACC TGATAATGTT CCTGTGGCAG 
AAATGCAAAA TGGGAATTCA GAAGATCTAT TCAGCAAGAA TGGATTATTT CAAGTTTGTC 
CAGAAAGTCA AAACTAACAA ATGGATTCAG CTGCTTTGGC AGATAAATGA AATGATTAAT 
ACTTGTCAAC CAGAAGACCT CAACCCTGTG TTTGTAGCTG AAGTTGCATT ACGACTAAGT 
GAAATACTGG AGATTTTAGG AAAGCCAGAA AAAAAAAGTC CTGGCAGCGC AAGCAGAGTA 
ACTATAGAAT TCCAGCAACT TCCTGACAGT TTTCCACTAT TTAAGAAAAC ACCTAAAGAG 
CAGTTACTTT TGGCTCATGA AACTCTTGAG AAGGCAGTTA CTGTGATGGG GCTATGTCGT 
TCCCAAACTA CATTGCCTAA TGGAAAATCA GTATATGACC ATTGTCATAT CAAGTTTATC 
CCTCCAAGAG GAGGTGGAAC CACTGGCAAA CCAATCACTG ACAATACTTT CCTGATGGAC 
TTGCATCTAG AACTCCTTGT GAGTCAGCAC CGGATAGCTG CTACACTCAT TGATCAACTC 
AAAGCTTGGA ATTTTTCTCC AAAACCTGCC CATCCCAAAG AAACTGAAAC TACAACTACT 
AGTCATTTCA CAGAAGCATA CGTACTGGGC AAAATCAATA AGAATGAGTT ATCCAAAGCA 
GTCTTCTTAA TGATGAAAGC TCAGATACTA TTTGAGAAAG AAGATTCTCA GGTCATCAAA 
TCACTGGAGG AATCGTTGTT CTTAATTCGG AAGATTGAAT CAGAGCAAAA AAACCAGTAT 
TCACCTCAGC AGAGTACAGA AGAAGAAAAA CCCCAGAGCA AAGTTCCTGC TCCTCCCATA 
CTACTTTCAA GAACTCATTG TTCAGTGACA TTTAAACCTG ATTCATTTTT TTGTGGAGAA 
AAGGTCGCTT GGTATGTCAT TTTGGGACGT CCAGCAGGAA CATGCATGAG AAAAGTAAGA 
TTGAACGACC ATAGTCTTCT AAACTGTGGA GAACCAGTAC CTGCTACTAG TAAAACCGTT 
TTAGAAGTTG TTGGATTGGA GCCTAATGAA AAATACGTGT TTGCACTTGC TGGTTATTGC 
TCTGCTGGAA CACTTATTGG GCAAGGAATT GGACTGACAT CTAAGCCTAT TCTGATTTAC 
ATGCCACTTT CTACTGTTAC TATTCGCTTA CATCTGACTG AGATTGCCTT TCAACTCGGT 
AAATATTCAG TGGCCAAGAA AGCCTTTGCA CCTGTTTGGG ACTATTTCGT CGATCCTCCT 
CTTCCAGAAA ATCCACACAT TTTTTCTTTA AGTAGCTTGT TAACTGTCAC ACCATTCAGG 
TTACATCTGG AGACCGTGTC AGAGTCTTCA TCAATCCTCT TATACTATTT CATTAGATGC 
ATTTTCATAA TAAGTAATAT TAATATTATA GAAAAAAAGC TCTTCTGTGA TACTATTAGA 
GGAGATGAGG TTTTCACTTG TCAACAAGTT TTCAGACTAC TTGAATGTCA GAGAATCCTT 
GTGGTCATTG AACTTAGTAC CCATGTAAAT AATTTTAGTT ATTCCCTACA AGCTGTTGTT 
CACTGCTATG GTCTCCTGGC CCCTCTAATC TTTCACAATA TTGTCCTGGT TCCAATAATC 
CAGGTCCTAA TTAAGTGTTT GGTAATTTTG CAAGAAATCC CAGGTGTTAC CTTTCCAAAG 
AGACAGATGG GGAATTTTGA GTCTACCCAG CATATGATTG CGTGCACTGT TTATTACACG 
GCAAAGGTGT TACGTTCATG GAAGGAATTT GACCTTGCAG TGATTATTAT AAATTTGGGG 
AAAAAGTTAC TGGACACCTC ACAAGGGTTA TTTTCAAATG TCACTAAAGC TGCCTTTGGT 
GAGGATGATC AAGATGACTT ATCAGAGGAG GCCTACTCAA CCAAGAGAAT CAAGAGGGTC 
AAGGGACAGA TTGCCATACT GGACAAAATC AGCGAGCAGC TCAACGTCAT GGAAGCTCAA 
CTACTCAAAC TGACGAAACA AAGTCCCATG TATGAGCTCA CAGGAACAGA AGATCCCTTT 
TTCCTCTACC CAATAATTCT AACTTGGACT CTCAAAACTG CGATGAGGGA AGTAATGAAA 
TTCAAGAGTA AACCTCGTTT TCTGGAATTC TTTGTTCAAG TTATGACAAA ATGTCTTATT 
GAAGAGAAAT TTCACTATGT GGTGGAATTG GCATTTTCAG TTCAACTCTT CCTTAAAAGG 
AGAAATGCCA GTTTACTTGG AGTAAGGAGA TCTCCAGTTC TTCACACTCT TCCATCTAAT 
GAAGAACCAA AGAAATACAA GGCAGCTGCC ATGGAGATTC AAAAAACTAC AGATGTATCA 
ATTGGGAAGT CAAAATCAAA GAAAAAAGGT ATTCTGCAAG ATTATTTTGT CAAGCATCTC 
TCACTTTCTG AATTGCCAGA TTTGGAAAAA TTTAAAAGAG CTGATGTGAG AAAAGTAGCT 
ATAAAAATCC TTTCAGAACA CTTGGCCCCA AAACTAAATC GCTATATAAA AAGGAAAAGA 
ATGCATCGAA TATTTATTGA AGAGTTACCC TGGAAGTCTC AGATGAATGT ACATTTGGCT 
GAAGCTCACT TCAACTTATT CAAAATCAGA TTCATGGAGC GTAAGAAGGT TAAAGCTTGC 
ACTTCACAAA ATACAATGAG CTACCGAATT TTAGACCCGG ATATCTTCTC AATGTACAAT 
TCTGGGACGT TGTTAGTGGA TGAAAACTTA AATTCAGATA ATTACCAAGC ATTGCTTGAT 
TTCATGCTTT CAAGAAAAAT GAGAAAGATC AATCTCTCAG ATGCCAATGA AATTTCTTTT 
GGTGGTGTCA GATTGAGTGA AGATATCCCT AAAGGGGTAA ACACAAATGA CCTGGAATCC 
GCTAGAGAGA AAACAGCAAA TCTTGGAATC ATAGACCATT TTATCAGGAC CTTTGTTCAC 
TGCAAAAGAG CATTGGTTCT TGCTCATCGT GGTGGTTTCT GGACCTTACT TCAGAATGTG 
ACCCGGACTC TTTGGAATTA CACCATAGAG ATGCAGTTAC TCTATAAACA GGCGCAGTTT 
CTTTCCAAAA CATTCCCTTT CAGCAAGGAT ATTATGATAT GCACCTTCGT GATGCCATTT 
ACTTTGGGAG CAGATGCCCT TATTGACATG CTGTTAGACC TGCAGAATAC CAAATCTCTT 
AAGATTGTGG ATGACTGTAA AGATTTTGGT GTTGCAAGTG CTTATGGGAA CATCACAGAG 
GATAATGGTG GCTTCAATCT GACATTTGAA GAACCTTTTG ATGATATGAA TTTGGTTGAC 
TTGAAATTCA TTTCTGACTT CATCTTTAAG ACTTTGGAAA TCTTGTACAG AGTGGGAAAA 
TGGGAAACAC TAGTCTACAT TGCCCTTCAG TTCAATGAAG TGACACATGA GAGATACACT 
GAGCAAGTGA TGCCATTGTT AATCTATTCT CAAAGACAGC TTTTGCAGAG ACTCCAGAGA 
AATAGTGGGG AGCAGACAGA TAATATAGAA TGGCAGTTTG ATGACACGGT AAACCTCCGA 
GATGTCATTG GGAAACTGCC CAATTCTTCT TTTGTGCAGA CTATAAAGGG CAAGTCTAAA 
AGACTTTTTA ATTATGAGCA AGGTGTAGAT CTTTCGGAGA CATACATTGC AAATAGAATA 
GTGTCTGTGC CCCTGAATGT GAAAGAAACT TTGAAGACCT TTAGAGAAAC CTTGACGAAA 
TCTAAGTACC ACAACAGATC ACTGAGATAC AGCAGAAAAT TGCTTTCTTT GTTACTGGCC 
CACACACAAG AAGCCAGTGG AGGAATAAAC AATCCCAAAT TTATGTATGG AAAAGTGGAA 
TTTAGCCTTG GAACTGAAGA AATACATTCG CCTACACCCC CTGATCTTTT TAATGAAAAG 
TTCACTGCTT CTGTTACAGT ACAGAGCACA AAAATTTCTC CTTTCCAATT TCAACTTGTT 
ATCACTTCTT ATGAGAAAAC GATCGAGATT CTTGAAATCA GCAATCAAAC AGATCTCAAG 
ATACAGGCAC TACATGAGCT TGGAAATCTT CACATCTATG CAGAAAACAG AAGGGCAGCT 
TTTAAATGCT GGAGTCAAGC TCTTGATGCC ATACTCAAAA AAAAAGATGT TCTACATACG 
TGGAAAGAAA TTGTCAGCCC CATCCAAAAA CCTATGAATA GCATCGTGGG TTCAAACTAC 
ATCGATTATA GTGAAGATTT TCTGTTGAAA GCTGGCATTT GGGGATGCTT GCAGGGAGGA 
GTCATAGCAG CAAAGATGGC CCAATACATT TTAACATCAA ATATGAATGA GAGGATTGAA 
TGTTGCATTT TCTCTTCATT TTTTTTTAAG GGTCTACTTA GAACCTCTCT TGTCCATCCC 
AAATATGACC ATGAGTATGC CCGTTATGAA ATCACCCAAC TTACTCCAGG GCTGGATCTC 
TTCTGTGACC GATACAGAGC AGATGTGTCC ATTGTCCTCA GCAGCCTGAG TTACCTCTGT 
CATGAACTCC ATTCTGTCCA CCAAGACCTT ATTCTTCTGC CACTTATTTC ATTAAACCAT 
TACTTGGTCT GTGAAGTTTG CCAAGATCTA TTTAAAACCA TAGATACAAG GATCCTTAAG 
ACGGAAATCC TTATTGATTT AGGATTCTTT TCTGAAGCTT TTAGTGAAAT GGCTGGTCTT 
TATACTGGAA AAAACATCCC CACTTCCACG CCCTTAGGCT ATAAACTTCC AAAAACTAAG 
ATCATGCAGC CCTTTCAAGC AGGACACTCT TATTTGAGCA AAGAAAATAT ACAGGCCCTG 
GAAGATTTAA TGAATCAAGG CATCCCCCTA TACATTATTT CGATTTGCCA ACCATCTTTC 
TCCTATAAGT TTACTTTGGT CAAAATGCGC TTTTTAATCA GTTTGGCTGC AACCATAAAT 
TGCTTCCCAG TTAGGGATAC AACAGCATTC TTCCAGGACT TTACCCAGAA GAGCAAAGCC 
AGTGCTTCAG TCTTTAAAGG TACAAACCCT GAAGATTCAA TAATCCCCTC TCAAGAGACA 
GTTCAAAATG ACTTTACCGC AGCTGAAGGA ACACAGAGAG CTCTGGACAA GAGCATTGAC 
TTCTCAAAGC AAAGAGGCTT CGATGATATG AGGAAGATGC TGATGGTGGA CCCAGAATAT 
TTAAAGGATG AAACCCCAGC AAATGTTAAC CTGACCAAGT CAAAAGAAGA GTTGTCTCCA 
GGTAAACTAA AGGGGATTTT ATTGGCAGAA GCTGAGGACA AGCTTCTCAT GCTAATAGAA 
AATGCAGAGG AATTGAGTCA CAAGAAGATA ACTCAGTTTA GCCCAATAGA ATTGGAGATT 
ATGATAGAAG TCAAGTTACA GCTAGCCGCT ATTGCGATGC AGAGACAACA AGCCCCACTG 
AGCACTTCAA TAGCTTTCAC TGCAATTAAA ATCCTTCAGG ATGCAGACAT TTTCAAGAAG 
ACTAAAGAAG AACTGTGTGC GAATGATGAT GAACTGGATG CCATGGAGTC TGTGGAGATG 
TTGGCCCGAG AACGTCTAAA CATCCATCTA TGGCTAAAGT GTCGTCTGGC CTTGCTGGTG 
GCCACTGTGA CCCACATCCG TGGCATGGGG GTCGTGAAAG AAAATGAGAT GATTGAGTGC 
AGACTTTTGA TAAATGAAGT ATTTGTGGAG GCTCAAAACT GGAATGATAC TGAGATGCAA 
GCAGAAGTCA TGGTGCAGGC TGTCAAGTTT GACCTGCAAG AAAGACATTC TGCAGCCAAC 
ATCTTAGAAC ATTTGAAGGA TATAAGAGGC TTACTTGAAG GAAAATCCTT TGTCTCTCCT 
CGAGGCTCTT TAATACTTAT GAAAAGCCTG CTGTTAATGG ATGATTTAAT GAAAATTGAG 
ATGCCCCAGG AATATCTCTT CCTTAAAAAT GAAAATCTCA ATTTACTAAA TCGAGCTCAT 
GAGCTTATGA TTGCGCAGGC TCTATCTTTG GGTGAAAACA TTTTATTGCC TTGTTCAGAC 
ACTGAATATG CAAATATCTT CTACCCTTTG AAAAACGTTT ATTTTCCATA CATCAACTTA 
CTTGCAAAAA CAAAAATGAG GATTGGGCAT ATGTTAGCCA AGAGCACTGC TGCCTGTGAG 
AGAAAAGAAC CTCAGAAGTG GGAACCCATT CGTCATCTCT TTGAGACAGC ATTGCAACTT 
TGTAGGGCAG CTGCAGTGAA GGAACGGGAG TTAGAAGCCG AAATCCTTTT TGAGAAAGGC 
AAAGTAGAAC GTCAAATGCT TGTTGTACAA GACAACAAGC CTTTAATTGG TGATCTTTTT 
GATGCTATAA AAATAAGTCT ACAAAATGAT GAAAATTCAG GGTTGATAAG GAAATCCTAT 
CTGGAAATCG CCCTGTTATA TTTACTTGTG TGGAATTATA GGAAAAATGC TTCAAAAAAT 
AAGATCTTTC TCCAAGTCCA GAATCCATCA AAGGAATCAC TAGAAATTAA AACTGAAATT 
TACCTGGCAA TGTCCTGGAT AGCAATCCGA GCTGCTGCAC AGGTCAGTGA AGCTACAATG 
GCAGCTAAGA AGCTAACTGG AGAAAAGAGT GTGAAGACCA AGAAAATGGA TCAAAATGTC 
TATCAGAATA TCCCTGAATT TGCTCATGCT GACCTCACTT GTACCTATGT AGATTACTTG 
ACTGCTAACT ATAAAACTTG TGTCAAAACT TCCTATACCA CTCCGAGCGA ATCTGGAAGT 
TATTTAAGTA TGGAACAAGA TCTGTACACC AACCCCAGTG AAGAAAATGA GGAGCCCAGC 
CAGCTGGAGA TCACCTGGGT CCATCTGGCC CGTTACTACA CTCACCTGTT AAGAGTTAAC 
AATATGAATA CCTTGCTTGC TCCTCCAAAG CCAGTTTTCT TAACAGATGA AATTTTCTAC 
ACTTCTGTTT TCAATGAAGA TCTGATTATA CGTCAGAAAG AGATGAAGAA TTTCCTCAAA 
AAATTCTTAA GTGTTTTTAC TTCAGCCTGT CTAGAGTGTT TCCCCAAAGA CCTCCTTCTT 
GGAATAGAAA GACTAAATAT TCCAGATAAT GCCGAACTTA GGCAATCACT TAAGACATTT 
CAAGATGTGA GTGCTATAAT AGAAAAAATA GTCACCACTA AGATCAACCC CATTATCCTT 
CCTCCATCTC CCCCGCATGA ACATATGGAT TTTGCTATAC ATGCAGAAAG CCCAGATCTT 
TGCTTCCAGT GGTATATACC CCCTCTGGAA AAACCTCCAA GTGATAAAGA ACCCATGGTC 
ATGTTGCTGT ATGCCTATAA CACCAAACCT GTCAAGATTA ATGATATCTT TGAATTCAAT 
ATCTCCTCGA TATTTCCTGG GTTTTTATGG CTTCCTCTGA ACAAAGTTAT AGCCTTACAT 
GAAGCTCTAT CTGGCCTTAG GCAAGAAACT GAAATGTTGT TATACACAAA AAAATTGCCT 
ACAGAAGAAA AGAAGTCCCT ACTACCCTTC AGTGCCTTAA AACCTGCTAG AGAAGTCTCT 
GAAGAGATCG TCCTCAGTAA GCAAATGAAG ACTGTGTTTT ATCAGAGTTG CACTGAAATA 
GCAAGACTGC TTTCGAGAAA TGAAGACATA CCCCTAATAA GTGAGGTTCC ATTTGAAGAA 
TCTTTGCCAA GTATACAGAA GTTGGAGATG CTTTTTGACC CCTCCAGTGG CTCTGTCTTA 
AAGGCAGAAG CCATTTTCAA CTGGATCGTG TCTTTTCTTA CATCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007503306.1
CDS	194..10024
Translation	MDTLKVPGFVSPSGSASGSVSSMTSVSDYSWSQSSEGDKGDTEVTLQVPSSRSFLTRLPKTRGSPFRGLGQPLTAAPATTASRRRRRDPFLESIAMMNVMRQMPIKALRKFPVMERKSSECRCLLRQRPRPCNLIEQPRAVFYGPLDAKNPLLISFQKELKGVLDFLKRKQSQTHTPEEYQEYQQRGTFTLFNIWFKYQNRLPVQYYHEHLLKTGDTLIQAKEYKLALIQCYGKYLSQFNPSHDESDMNVTTFKKVFFSKGVNDESAKLTFHALQGKCISQYQIICATDPCFKRELSVEQCFKILSVFRLMMQITLPYEHLCWIVYNGTIHIYTICRKLMTIGQSAKALEFLLWASICMESSLPLLAVSYLSWRATLYTAVCQCYYDCHAGMQGEIFARRGLGKIDDLLRLEVMSITPPQEEERKKFREATIKMAVMIFKRGVFEPRRKSKSYVRTKLRLNLREAQNLQWPRTATEKLLSEMFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_007503244.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007503244.2

ORF Insert Sequence:

ATGGATACCC TGAAAGTCCC CGGCTTCGTG TCCCCAAGCG GCTCAGCCTC AGGCTCTGTC 
AGCTCTATGA CGTCGGTGTC GGACTACTCC TGGAGCCAGT CCTCGGAGGG GGACAAGGGC 
GATACGGAGG TGACGCTCCA AGTCCCCTCG TCCCGCAGCT TCTTGACCCG GCTCCCCAAG 
ACCCGAGGGT CTCCCTTTCG AGGACTCGGG CAACCCTTGA CGGCGGCCCC CGCGACTACG 
GCTTCCCGCC GGCGCCGCCG TGATCCCTTC TTGGAGTCAA TAGCGATGAT GAACGTCATG 
AGGCAGATGC CAATCAAGGC GCTTCGCAAG TTTCCGGTCA TGGAGAGGAA GAGCTCTGAG 
TGCCGCTGCC TACTCCGGCA ACGACCTCGT CCTTGCAACC TTATTGAGCA GCCCAGAGCT 
GTCTTCTATG GGCCCCTGGA CGCCAAAAAC CCGCTGCTTA TCTCCTTCCA GAAGGAGCTG 
AAGGGCGTGC TGGACTTCCT CAAGAGGAAG CAGAGTCAGA CCCACACTCC CGAGGAATAC 
CAGGAGTACC AGCAACGCGG TACCTTCACC TTGTTCAATA TCTGGTTCAA GTATCAGAAC 
CGCCTGCCAG TCCAGTATTA CCATGAGCAT CTTTTGAAGA CTGGAGACAC CCTTATTCAA 
GCAAAAGAAT ACAAGTTGGC CTTAATACAA TGCTATGGGA AGTATCTTAG TCAGTTCAAT 
CCCAGTCACG ATGAGAGTGA CATGAACGTG ACAACTTTCA AAAAAGTCTT TTTCTCCAAA 
GGTGTCAATG ATGAAAGCGC CAAACTTACA TTTCACGCTT TGCAAGGGAA ATGTATTAGC 
CAGTACCAAA TTATCTGTGC CACCGATCCC TGTTTCAAGA GGGAGCTGTC TGTGGAGCAG 
TGCTTCAAGA TTCTGTCGGT TTTTCGGCTC ATGATGCAGA TAACCCTGCC CTACGAACAC 
CTTTGCTGGA TCGTGTACAA TGGTACAATT CACATTTATA CAATTTGCAG GAAGCTGATG 
ACCATTGGAC AGTCTGCTAA GGCCTTGGAA TTCTTGCTCT GGGCCAGTAT TTGTATGGAA 
TCCTCTTTAC CCCTCTTAGC AGTAAGCTAT CTCAGTTGGA GAGCTACCCT GTATACTGCT 
GTTTGCCAGT GCTACTATGA CTGCCATGCT GGAATGCAGG GAGAGATATT TGCTCGGCGA 
GGTTTGGGAA AAATTGATGA TTTATTACGT CTAGAAGTAA TGAGTATCAC ACCACCACAG 
GAGGAAGAAA GAAAAAAATT CCGAGAGGCC ACCATCAAGA TGGCGGTGAT GATCTTTAAG 
AGAGGAGTCT TTGAACCACG AAGAAAGAGT AAGTCTTATG TAAGAACAAA ACTGAGACTG 
AACCTACGGG AAGCACAGAA TTTGCAATGG CCACGCACAG CTACAGAGAA GTTGTTGAGT 
GAGATGTTTG ATTGTGGATC AGCCCAGTTT TTGGCTGTCT TGGAAGCCCT TTCTGAACCA 
AATAGAAGAG TGTTACAAAC TGGTCACTTG TCAGAGGAAA TGGAAGTTCG TGAGGTCATC 
GCTGAGCTCT TCATGGCAGC AAGCGAGCTT TTATCAGATG CATGTAGTAG CCATGGGAAA 
TCCAGTTTTT CCAAAAACAT CTCTCTACAG CTTTCTCAAA TGCAACTCGC CATTGAAAGC 
AAACAAAGTG TTTCAATAGC TGCTGCAGTG AAGTTGATAA AATTATCTTT CACCTTTGAG 
GAATGGCATT TGTTTAATTC TGCTGCTTCT CAGTTTATCG GTTTTCTTCA GCTTCAAAGT 
GGCCCACACT TGCAGAAAGC CAGGAAGGAT TTAATGCTTC TGGCTGCGAT GGAACCGCTA 
ATTAATGTGA AGCGGAATAA AGGCTTAAGC TATGCTGGTG AAGGAGGCAG CACTACCACT 
GAAGGAGCTC GAAGCTCAGG GAGAAAAATT GTGTTTCAAG AAAATTTCTC ATTATCTGGA 
AGCTATTCTG ATGATATTTT CTATTTGGCA TCGATGCTGT TTTCCTTCAT TTGTTCACCT 
ACTGAGAAAA TTCGTCCTGA TGAAGAGATC GTTGTTGACC TGATAATGTT CCTGTGGCAG 
AAATGCAAAA TGGGAATTCA GAAGATCTAT TCAGCAAGAA TGGATTATTT CAAGTTTGTC 
CAGAAAGTCA AAACTAACAA ATGGATTCAG CTGCTTTGGC AGATAAATGA AATGATTAAT 
ACTTGTCAAC CAGAAGACCT CAACCCTGTG TTTGTAGCTG AAGTTGCATT ACGACTAAGT 
GAAATACTGG AGATTTTAGG AAAGCCAGAA AAAAAAAGTC CTGGCAGCGC AAGCAGAGTA 
ACTATAGAAT TCCAGCAACT TCCTGACAGT TTTCCACTAT TTAAGAAAAC ACCTAAAGAG 
CAGTTACTTT TGGCTCATGA AACTCTTGAG AAGGCAGTTA CTGTGATGGG GCTATGTCGT 
TCCCAAACTA CATTGCCTAA TGGAAAATCA GTATATGACC ATTGTCATAT CAAGTTTATC 
CCTCCAAGAG GAGGTGGAAC CACTGGCAAA CCAATCACTG ACAATACTTT CCTGATGGAC 
TTGCATCTAG AACTCCTTGT GAGTCAGCAC CGGATAGCTG CTACACTCAT TGATCAACTC 
AAAGCTTGGA ATTTTTCTCC AAAACCTGCC CATCCCAAAG AAACTGAAAC TACAACTACT 
AGTCATTTCA CAGAAGCATA CGTACTGGGC AAAATCAATA AGAATGAGTT ATCCAAAGCA 
GTCTTCTTAA TGATGAAAGC TCAGATACTA TTTGAGAAAG AAGATTCTCA GGTCATCAAA 
TCACTGGAGG AATCGTTGTT CTTAATTCGG AAGATTGAAT CAGAGCAAAA AAACCAGTAT 
TCACCTCAGC AGAGTACAGA AGAAGAAAAA CCCCAGAGCA AAGTTCCTGC TCCTCCCATA 
CTACTTTCAA GAACTCATTG TTCAGTGACA TTTAAACCTG ATTCATTTTT TTGTGGAGAA 
AAGGTCGCTT GGTATGTCAT TTTGGGACGT CCAGCAGGAA CATGCATGAG AAAAGTAAGA 
TTGAACGACC ATAGTCTTCT AAACTGTGGA GAACCAGTAC CTGCTACTAG TAAAACCGTT 
TTAGAAGTTG TTGGATTGGA GCCTAATGAA AAATACGTGT TTGCACTTGC TGGTTATTGC 
TCTGCTGGAA CACTTATTGG GCAAGGAATT GGACTGACAT CTAAGCCTAT TCTGATTTAC 
ATGCCACTTT CTACTGTTAC TATTCGCTTA CATCTGACTG AGATTGCCTT TCAACTCGGT 
AAATATTCAG TGGCCAAGAA AGCCTTTGCA CCTGTTTGGG ACTATTTCGT CGATCCTCCT 
CTTCCAGAAA ATCCACACAT TTTTTCTTTA AGTAGCTTGT TAACTGTCAC ACCATTCAGG 
TTACATCTGG AGACCGTGTC AGAGTCTTCA TCAATCCTCT TATACTATTT CATTAGATGC 
ATTTTCATAA TAAGTAATAT TAATATTATA GAAAAAAAGC TCTTCTGTGA TACTATTAGA 
GGAGATGAGG TTTTCACTTG TCAACAAGTT TTCAGACTAC TTGAATGTCA GAGAATCCTT 
GTGGTCATTG AACTTAGTAC CCATGTAAAT AATTTTAGTT ATTCCCTACA AGCTGTTGTT 
CACTGCTATG GTCTCCTGGC CCCTCTAATC TTTCACAATA TTGTCCTGGT TCCAATAATC 
CAGGTCCTAA TTAAGTGTTT GGTAATTTTG CAAGAAATCC CAGGTGTTAC CTTTCCAAAG 
AGACAGATGG GGAATTTTGA GTCTACCCAG CATATGATTG CGTGCACTGT TTATTACACG 
GCAAAGGTGT TACGTTCATG GAAGGAATTT GACCTTGCAG TGATTATTAT AAATTTGGGG 
AAAAAGTTAC TGGACACCTC ACAAGGGTTA TTTTCAAATG TCACTAAAGC TGCCTTTGGT 
GAGGATGATC AAGATGACTT ATCAGAGGAG GCCTACTCAA CCAAGAGAAT CAAGAGGGTC 
AAGGGACAGA TTGCCATACT GGACAAAATC AGCGAGCAGC TCAACGTCAT GGAAGCTCAA 
CTACTCAAAC TGACGAAACA AAGTCCCATG TATGAGCTCA CAGGAACAGA AGATCCCTTT 
TTCCTCTACC CAATAATTCT AACTTGGACT CTCAAAACTG CGATGAGGGA AGTAATGAAA 
TTCAAGAGTA AACCTCGTTT TCTGGAATTC TTTGTTCAAG TTATGACAAA ATGTCTTATT 
GAAGAGAAAT TTCACTATGT GGTGGAATTG GCATTTTCAG TTCAACTCTT CCTTAAAAGG 
AGAAATGCCA GTTTACTTGG AGTAAGGAGA TCTCCAGTTC TTCACACTCT TCCATCTAAT 
GAAGAACCAA AGAAATACAA GGCAGCTGCC ATGGAGATTC AAAAAACTAC AGATGTATCA 
ATTGGGAAGT CAAAATCAAA GAAAAAAGGT ATTCTGCAAG ATTATTTTGT CAAGCATCTC 
TCACTTTCTG AATTGCCAGA TTTGGAAAAA TTTAAAAGAG CTGATGTGAG AAAAGTAGCT 
ATAAAAATCC TTTCAGAACA CTTGGCCCCA AAACTAAATC GCTATATAAA AAGGAAAAGA 
ATGCATCGAA TATTTATTGA AGAGTTACCC TGGAAGTCTC AGATGAATGT ACATTTGGCT 
GAAGCTCACT TCAACTTATT CAAAATCAGA TTCATGGAGC GTAAGAAGGT TAAAGCTTGC 
ACTTCACAAA ATACAATGAG CTACCGAATT TTAGACCCGG ATATCTTCTC AATGTACAAT 
TCTGGGACGT TGTTAGTGGA TGAAAACTTA AATTCAGATA ATTACCAAGC ATTGCTTGAT 
TTCATGCTTT CAAGAAAAAT GAGAAAGATC AATCTCTCAG ATGCCAATGA AATTTCTTTT 
GGTGGTGTCA GATTGAGTGA AGATATCCCT AAAGGGGTAA ACACAAATGA CCTGGAATCC 
GCTAGAGAGA AAACAGCAAA TCTTGGAATC ATAGACCATT TTATCAGGAC CTTTGTTCAC 
TGCAAAAGAG CATTGGTTCT TGCTCATCGT GGTGGTTTCT GGACCTTACT TCAGAATGTG 
ACCCGGACTC TTTGGAATTA CACCATAGAG ATGCAGTTAC TCTATAAACA GGCGCAGTTT 
CTTTCCAAAA CATTCCCTTT CAGCAAGGAT ATTATGATAT GCACCTTCGT GATGCCATTT 
ACTTTGGGAG CAGATGCCCT TATTGACATG CTGTTAGACC TGCAGAATAC CAAATCTCTT 
AAGATTGTGG ATGACTGTAA AGATTTTGGT GTTGCAAGTG CTTATGGGAA CATCACAGAG 
GATAATGGTG GCTTCAATCT GACATTTGAA GAACCTTTTG ATGATATGAA TTTGGTTGAC 
TTGAAATTCA TTTCTGACTT CATCTTTAAG ACTTTGGAAA TCTTGTACAG AGTGGGAAAA 
TGGGAAACAC TAGTCTACAT TGCCCTTCAG TTCAATGAAG TGACACATGA GAGATACACT 
GAGCAAGTGA TGCCATTGTT AATCTATTCT CAAAGACAGC TTTTGCAGAG ACTCCAGAGA 
AATAGTGGGG AGCAGACAGA TAATATAGAA TGGCAGTTTG ATGACACGGT AAACCTCCGA 
GATGTCATTG GGAAACTGCC CAATTCTTCT TTTGTGCAGA CTATAAAGGG CAAGTCTAAA 
AGACTTTTTA ATTATGAGCA AGGTGTAGAT CTTTCGGAGA CATACATTGC AAATAGAATA 
GTGTCTGTGC CCCTGAATGT GAAAGAAACT TTGAAGACCT TTAGAGAAAC CTTGACGAAA 
TCTAAGTACC ACAACAGATC ACTGAGATAC AGCAGAAAAT TGCTTTCTTT GTTACTGGCC 
CACACACAAG AAGCCAGTGG AGGAATAAAC AATCCCAAAT TTATGTATGG AAAAGTGGAA 
TTTAGCCTTG GAACTGAAGA AATACATTCG CCTACACCCC CTGATCTTTT TAATGAAAAG 
TTCACTGCTT CTGTTACAGT ACAGAGCACA AAAATTTCTC CTTTCCAATT TCAACTTGTT 
ATCACTTCTT ATGAGAAAAC GATCGAGATT CTTGAAATCA GCAATCAAAC AGATCTCAAG 
ATACAGGCAC TACATGAGCT TGGAAATCTT CACATCTATG CAGAAAACAG AAGGGCAGCT 
TTTAAATGCT GGAGTCAAGC TCTTGATGCC ATACTCAAAA AAAAAGATGT TCTACATACG 
TGGAAAGAAA TTGTCAGCCC CATCCAAAAA CCTATGAATA GCATCGTGGG TTCAAACTAC 
ATCGATTATA GTGAAGATTT TCTGTTGAAA GCTGGCATTT GGGGATGCTT GCAGGGAGGA 
GTCATAGCAG CAAAGATGGC CCAATACATT TTAACATCAA ATATGAATGA GAGGATTGAA 
TGTTGCATTT TCTCTTCATT TTTTTTTAAG GGTCTACTTA GAACCTCTCT TGTCCATCCC 
AAATATGACC ATGAGTATGC CCGTTATGAA ATCACCCAAC TTACTCCAGG GCTGGATCTC 
TTCTGTGACC GATACAGAGC AGATGTGTCC ATTGTCCTCA GCAGCCTGAG TTACCTCTGT 
CATGAACTCC ATTCTGTCCA CCAAGACCTT ATTCTTCTGC CACTTATTTC ATTAAACCAT 
TACTTGGTCT GTGAAGTTTG CCAAGATCTA TTTAAAACCA TAGATACAAG GATCCTTAAG 
ACGGAAATCC TTATTGATTT AGGATTCTTT TCTGAAGCTT TTAGTGAAAT GGCTGGTCTT 
TATACTGGAA AAAACATCCC CACTTCCACG CCCTTAGGCT ATAAACTTCC AAAAACTAAG 
ATCATGCAGC CCTTTCAAGC AGGACACTCT TATTTGAGCA AAGAAAATAT ACAGGCCCTG 
GAAGATTTAA TGAATCAAGG CATCCCCCTA TACATTATTT CGATTTGCCA ACCATCTTTC 
TCCTATAAGT TTACTTTGGT CAAAATGCGC TTTTTAATCA GTTTGGCTGC AACCATAAAT 
TGCTTCCCAG TTAGGGATAC AACAGCATTC TTCCAGGACT TTACCCAGAA GAGCAAAGCC 
AGTGCTTCAG TCTTTAAAGG TACAAACCCT GAAGATTCAA TAATCCCCTC TCAAGAGACA 
GTTCAAAATG ACTTTACCGC AGCTGAAGGA ACACAGAGAG CTCTGGACAA GAGCATTGAC 
TTCTCAAAGC AAAGAGGCTT CGATGATATG AGGAAGATGC TGATGGTGGA CCCAGAATAT 
TTAAAGGATG AAACCCCAGC AAATGTTAAC CTGACCAAGT CAAAAGAAGA GTTGTCTCCA 
GGTAAACTAA AGGGGATTTT ATTGGCAGAA GCTGAGGACA AGCTTCTCAT GCTAATAGAA 
AATGCAGAGG AATTGAGTCA CAAGAAGATA ACTCAGTTTA GCCCAATAGA ATTGGAGATT 
ATGATAGAAG TCAAGTTACA GCTAGCCGCT ATTGCGATGC AGAGACAACA AGCCCCACTG 
AGCACTTCAA TAGCTTTCAC TGCAATTAAA ATCCTTCAGG ATGCAGACAT TTTCAAGAAG 
ACTAAAGAAG AACTGTGTGC GAATGATGAT GAACTGGATG CCATGGAGTC TGTGGAGATG 
TTGGCCCGAG AACGTCTAAA CATCCATCTA TGGCTAAAGT GTCGTCTGGC CTTGCTGGTG 
GCCACTGTGA CCCACATCCG TGGCATGGGG GTCGTGAAAG AAAATGAGAT GATTGAGTGC 
AGACTTTTGA TAAATGAAGT ATTTGTGGAG GCTCAAAACT GGAATGATAC TGAGATGCAA 
GCAGAAGTCA TGGTGCAGGC TGTCAAGTTT GACCTGCAAG AAAGACATTC TGCAGCCAAC 
ATCTTAGAAC ATTTGAAGGA TATAAGAGGC TTACTTGAAG GAAAATCCTT TGTCTCTCCT 
CGAGGCTCTT TAATACTTAT GAAAAGCCTG CTGTTAATGG ATGATTTAAT GAAAATTGAG 
ATGCCCCAGG AATATCTCTT CCTTAAAAAT GAAAATCTCA ATTTACTAAA TCGAGCTCAT 
GAGCTTATGA TTGCGCAGGC TCTATCTTTG GGTGAAAACA TTTTATTGCC TTGTTCAGAC 
ACTGAATATG CAAATATCTT CTACCCTTTG AAAAACGTTT ATTTTCCATA CATCAACTTA 
CTTGCAAAAA CAAAAATGAG GATTGGGCAT ATGTTAGCCA AGAGCACTGC TGCCTGTGAG 
AGAAAAGAAC CTCAGAAGTG GGAACCCATT CGTCATCTCT TTGAGACAGC ATTGCAACTT 
TGTAGGGCAG CTGCAGTGAA GGAACGGGAG TTAGAAGCCG AAATCCTTTT TGAGAAAGGC 
AAAGTAGAAC GTCAAATGCT TGTTGTACAA GACAACAAGC CTTTAATTGG TGATCTTTTT 
GATGCTATAA AAATAAGTCT ACAAAATGAT GAAAATTCAG GGTTGATAAG GAAATCCTAT 
CTGGAAATCG CCCTGTTATA TTTACTTGTG TGGAATTATA GGAAAAATGC TTCAAAAAAT 
AAGATCTTTC TCCAAGTCCA GAATCCATCA AAGGAATCAC TAGAAATTAA AACTGAAATT 
TACCTGGCAA TGTCCTGGAT AGCAATCCGA GCTGCTGCAC AGGTCAGTGA AGCTACAATG 
GCAGCTAAGA AGCTAACTGG AGAAAAGAGT GTGAAGACCA AGAAAATGGA TCAAAATGTC 
TATCAGAATA TCCCTGAATT TGCTCATGCT GACCTCACTT GTACCTATGT AGATTACTTG 
ACTGCTAACT ATAAAACTTG TGTCAAAACT TCCTATACCA CTCCGAGCGA ATCTGGAAGT 
TATTTAAGTA TGGAACAAGA TCTGTACACC AACCCCAGTG AAGAAAATGA GGAGCCCAGC 
CAGCTGGAGA TCACCTGGGT CCATCTGGCC CGTTACTACA CTCACCTGTT AAGAGTTAAC 
AATATGAATA CCTTGCTTGC TCCTCCAAAG CCAGTTTTCT TAACAGATGA AATTTTCTAC 
ACTTCTGTTT TCAATGAAGA TCTGATTATA CGTCAGAAAG AGATGAAGAA TTTCCTCAAA 
AAATTCTTAA GTGTTTTTAC TTCAGCCTGT CTAGAGTGTT TCCCCAAAGA CCTCCTTCTT 
GGAATAGAAA GACTAAATAT TCCAGATAAT GCCGAACTTA GGCAATCACT TAAGACATTT 
CAAGATGTGA GTGCTATAAT AGAAAAAATA GTCACCACTA AGATCAACCC CATTATCCTT 
CCTCCATCTC CCCCGCATGA ACATATGGAT TTTGCTATAC ATGCAGAAAG CCCAGATCTT 
TGCTTCCAGT GGTATATACC CCCTCTGGAA AAACCTCCAA GTGATAAAGA ACCCATGGTC 
ATGTTGCTGT ATGCCTATAA CACCAAACCT GTCAAGATTA ATGATATCTT TGAATTCAAT 
ATCTCCTCGA TATTTCCTGG GTTTTTATGG CTTCCTCTGA ACAAAGTTAT AGCCTTACAT 
GAAGCTCTAT CTGGCCTTAG GCAAGAAACT GAAATGTTGT TATACACAAA AAAATTGCCT 
ACAGAAGAAA AGAAGTCCCT ACTACCCTTC AGTGCCTTAA AACCTGCTAG AGAAGTCTCT 
GAAGAGATCG TCCTCAGTAA GCAAATGAAG ACTGTGTTTT ATCAGAGTTG CACTGAAATA 
GCAAGACTGC TTTCGAGAAA TGAAGACATA CCCCTAATAA GTGAGGTTCC ATTTGAAGAA 
TCTTTGCCAA GTATACAGAA GTTGGAGATG CTTTTTGACC CCTCCAGTGG CTCTGTCTTA 
AAGGCAGAAG CCATTTTCAA CTGGATCGTG TCTTTTCTTA CATCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP54 ( XM_007503246.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007503308.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA.

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CloneID	OMc32359
Clone ID Related Accession (Same CDS sequence)	XM_007503246.2
Accession Version	XM_007503246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007503246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTG GAGACATGGA GAGAAATCAG TGGGAAGCTG GGGATGAAGT GGAGAGCAAG 
ATCTTGTTGC AATGGCCACG CACAGCTACA GAGAAGTTGT TGAGTGAGAT GTTTGATTGT 
GGATCAGCCC AGTTTTTGGC TGTCTTGGAA GCCCTTTCTG AACCAAATAG AAGAGTGTTA 
CAAACTGGTC ACTTGTCAGA GGAAATGGAA GTTCGTGAGG TCATCGCTGA GCTCTTCATG 
GCAGCAAGCG AGCTTTTATC AGATGCATGT AGTAGCCATG GGAAATCCAG TTTTTCCAAA 
AACATCTCTC TACAGCTTTC TCAAATGCAA CTCGCCATTG AAAGCAAACA AAGTGTTTCA 
ATAGCTGCTG CAGTGAAGTT GATAAAATTA TCTTTCACCT TTGAGGAATG GCATTTGTTT 
AATTCTGCTG CTTCTCAGTT TATCGGTTTT CTTCAGCTTC AAAGTGGCCC ACACTTGCAG 
AAAGCCAGGA AGGATTTAAT GCTTCTGGCT GCGATGGAAC CGCTAATTAA TGTGAAGCGG 
AATAAAGGCT TAAGCTATGC TGGTGAAGGA GGCAGCACTA CCACTGAAGG AGCTCGAAGC 
TCAGGGAGAA AAATTGTGTT TCAAGAAAAT TTCTCATTAT CTGGAAGCTA TTCTGATGAT 
ATTTTCTATT TGGCATCGAT GCTGTTTTCC TTCATTTGTT CACCTACTGA GAAAATTCGT 
CCTGATGAAG AGATCGTTGT TGACCTGATA ATGTTCCTGT GGCAGAAATG CAAAATGGGA 
ATTCAGAAGA TCTATTCAGC AAGAATGGAT TATTTCAAGT TTGTCCAGAA AGTCAAAACT 
AACAAATGGA TTCAGCTGCT TTGGCAGATA AATGAAATGA TTAATACTTG TCAACCAGAA 
GACCTCAACC CTGTGTTTGT AGCTGAAGTT GCATTACGAC TAAGTGAAAT ACTGGAGATT 
TTAGGAAAGC CAGAAAAAAA AAGTCCTGGC AGCGCAAGCA GAGTAACTAT AGAATTCCAG 
CAACTTCCTG ACAGTTTTCC ACTATTTAAG AAAACACCTA AAGAGCAGTT ACTTTTGGCT 
CATGAAACTC TTGAGAAGGC AGTTACTGTG ATGGGGCTAT GTCGTTCCCA AACTACATTG 
CCTAATGGAA AATCAGTATA TGACCATTGT CATATCAAGT TTATCCCTCC AAGAGGAGGT 
GGAACCACTG GCAAACCAAT CACTGACAAT ACTTTCCTGA TGGACTTGCA TCTAGAACTC 
CTTGTGAGTC AGCACCGGAT AGCTGCTACA CTCATTGATC AACTCAAAGC TTGGAATTTT 
TCTCCAAAAC CTGCCCATCC CAAAGAAACT GAAACTACAA CTACTAGTCA TTTCACAGAA 
GCATACGTAC TGGGCAAAAT CAATAAGAAT GAGTTATCCA AAGCAGTCTT CTTAATGATG 
AAAGCTCAGA TACTATTTGA GAAAGAAGAT TCTCAGGTCA TCAAATCACT GGAGGAATCG 
TTGTTCTTAA TTCGGAAGAT TGAATCAGAG CAAAAAAACC AGTATTCACC TCAGCAGAGT 
ACAGAAGAAG AAAAACCCCA GAGCAAAGTT CCTGCTCCTC CCATACTACT TTCAAGAACT 
CATTGTTCAG TGACATTTAA ACCTGATTCA TTTTTTTGTG GAGAAAAGGT CGCTTGGTAT 
GTCATTTTGG GACGTCCAGC AGGAACATGC ATGAGAAAAG TAAGATTGAA CGACCATAGT 
CTTCTAAACT GTGGAGAACC AGTACCTGCT ACTAGTAAAA CCGTTTTAGA AGTTGTTGGA 
TTGGAGCCTA ATGAAAAATA CGTGTTTGCA CTTGCTGGTT ATTGCTCTGC TGGAACACTT 
ATTGGGCAAG GAATTGGACT GACATCTAAG CCTATTCTGA TTTACATGCC ACTTTCTACT 
GTTACTATTC GCTTACATCT GACTGAGATT GCCTTTCAAC TCGGTAAATA TTCAGTGGCC 
AAGAAAGCCT TTGCACCTGT TTGGGACTAT TTCGTCGATC CTCCTCTTCC AGAAAATCCA 
CACATTTTTT CTTTAAGTAG CTTGTTAACT GTCACACCAT TCAGGTTACA TCTGGAGACC 
GTGTCAGAGT CTTCATCAAT CCTCTTATAC TATTTCATTA GATGCATTTT CATAATAAGT 
AATATTAATA TTATAGAAAA AAAGCTCTTC TGTGATACTA TTAGAGGAGA TGAGGTTTTC 
ACTTGTCAAC AAGTTTTCAG ACTACTTGAA TGTCAGAGAA TCCTTGTGGT CATTGAACTT 
AGTACCCATG TAAATAATTT TAGTTATTCC CTACAAGCTG TTGTTCACTG CTATGGTCTC 
CTGGCCCCTC TAATCTTTCA CAATATTGTC CTGGTTCCAA TAATCCAGGT CCTAATTAAG 
TGTTTGGTAA TTTTGCAAGA AATCCCAGGT GTTACCTTTC CAAAGAGACA GATGGGGAAT 
TTTGAGTCTA CCCAGCATAT GATTGCGTGC ACTGTTTATT ACACGGCAAA GGTGTTACGT 
TCATGGAAGG AATTTGACCT TGCAGTGATT ATTATAAATT TGGGGAAAAA GTTACTGGAC 
ACCTCACAAG GGTTATTTTC AAATGTCACT AAAGCTGCCT TTGGTGAGGA TGATCAAGAT 
GACTTATCAG AGGAGGCCTA CTCAACCAAG AGAATCAAGA GGGTCAAGGG ACAGATTGCC 
ATACTGGACA AAATCAGCGA GCAGCTCAAC GTCATGGAAG CTCAACTACT CAAACTGACG 
AAACAAAGTC CCATGTATGA GCTCACAGGA ACAGAAGATC CCTTTTTCCT CTACCCAATA 
ATTCTAACTT GGACTCTCAA AACTGCGATG AGGGAAGTAA TGAAATTCAA GAGTAAACCT 
CGTTTTCTGG AATTCTTTGT TCAAGTTATG ACAAAATGTC TTATTGAAGA GAAATTTCAC 
TATGTGGTGG AATTGGCATT TTCAGTTCAA CTCTTCCTTA AAAGGAGAAA TGCCAGTTTA 
CTTGGAGTAA GGAGATCTCC AGTTCTTCAC ACTCTTCCAT CTAATGAAGA ACCAAAGAAA 
TACAAGGCAG CTGCCATGGA GATTCAAAAA ACTACAGATG TATCAATTGG GAAGTCAAAA 
TCAAAGAAAA AAGGTATTCT GCAAGATTAT TTTGTCAAGC ATCTCTCACT TTCTGAATTG 
CCAGATTTGG AAAAATTTAA AAGAGCTGAT GTGAGAAAAG TAGCTATAAA AATCCTTTCA 
GAACACTTGG CCCCAAAACT AAATCGCTAT ATAAAAAGGA AAAGAATGCA TCGAATATTT 
ATTGAAGAGT TACCCTGGAA GTCTCAGATG AATGTACATT TGGCTGAAGC TCACTTCAAC 
TTATTCAAAA TCAGATTCAT GGAGCGTAAG AAGGTTAAAG CTTGCACTTC ACAAAATACA 
ATGAGCTACC GAATTTTAGA CCCGGATATC TTCTCAATGT ACAATTCTGG GACGTTGTTA 
GTGGATGAAA ACTTAAATTC AGATAATTAC CAAGCATTGC TTGATTTCAT GCTTTCAAGA 
AAAATGAGAA AGATCAATCT CTCAGATGCC AATGAAATTT CTTTTGGTGG TGTCAGATTG 
AGTGAAGATA TCCCTAAAGG GGTAAACACA AATGACCTGG AATCCGCTAG AGAGAAAACA 
GCAAATCTTG GAATCATAGA CCATTTTATC AGGACCTTTG TTCACTGCAA AAGAGCATTG 
GTTCTTGCTC ATCGTGGTGG TTTCTGGACC TTACTTCAGA ATGTGACCCG GACTCTTTGG 
AATTACACCA TAGAGATGCA GTTACTCTAT AAACAGGCGC AGTTTCTTTC CAAAACATTC 
CCTTTCAGCA AGGATATTAT GATATGCACC TTCGTGATGC CATTTACTTT GGGAGCAGAT 
GCCCTTATTG ACATGCTGTT AGACCTGCAG AATACCAAAT CTCTTAAGAT TGTGGATGAC 
TGTAAAGATT TTGGTGTTGC AAGTGCTTAT GGGAACATCA CAGAGGATAA TGGTGGCTTC 
AATCTGACAT TTGAAGAACC TTTTGATGAT ATGAATTTGG TTGACTTGAA ATTCATTTCT 
GACTTCATCT TTAAGACTTT GGAAATCTTG TACAGAGTGG GAAAATGGGA AACACTAGTC 
TACATTGCCC TTCAGTTCAA TGAAGTGACA CATGAGAGAT ACACTGAGCA AGTGATGCCA 
TTGTTAATCT ATTCTCAAAG ACAGCTTTTG CAGAGACTCC AGAGAAATAG TGGGGAGCAG 
ACAGATAATA TAGAATGGCA GTTTGATGAC ACGGTAAACC TCCGAGATGT CATTGGGAAA 
CTGCCCAATT CTTCTTTTGT GCAGACTATA AAGGGCAAGT CTAAAAGACT TTTTAATTAT 
GAGCAAGGTG TAGATCTTTC GGAGACATAC ATTGCAAATA GAATAGTGTC TGTGCCCCTG 
AATGTGAAAG AAACTTTGAA GACCTTTAGA GAAACCTTGA CGAAATCTAA GTACCACAAC 
AGATCACTGA GATACAGCAG AAAATTGCTT TCTTTGTTAC TGGCCCACAC ACAAGAAGCC 
AGTGGAGGAA TAAACAATCC CAAATTTATG TATGGAAAAG TGGAATTTAG CCTTGGAACT 
GAAGAAATAC ATTCGCCTAC ACCCCCTGAT CTTTTTAATG AAAAGTTCAC TGCTTCTGTT 
ACAGTACAGA GCACAAAAAT TTCTCCTTTC CAATTTCAAC TTGTTATCAC TTCTTATGAG 
AAAACGATCG AGATTCTTGA AATCAGCAAT CAAACAGATC TCAAGATACA GGCACTACAT 
GAGCTTGGAA ATCTTCACAT CTATGCAGAA AACAGAAGGG CAGCTTTTAA ATGCTGGAGT 
CAAGCTCTTG ATGCCATACT CAAAAAAAAA GATGTTCTAC ATACGTGGAA AGAAATTGTC 
AGCCCCATCC AAAAACCTAT GAATAGCATC GTGGGTTCAA ACTACATCGA TTATAGTGAA 
GATTTTCTGT TGAAAGCTGG CATTTGGGGA TGCTTGCAGG GAGGAGTCAT AGCAGCAAAG 
ATGGCCCAAT ACATTTTAAC ATCAAATATG AATGAGAGGA TTGAATGTTG CATTTTCTCT 
TCATTTTTTT TTAAGGGTCT ACTTAGAACC TCTCTTGTCC ATCCCAAATA TGACCATGAG 
TATGCCCGTT ATGAAATCAC CCAACTTACT CCAGGGCTGG ATCTCTTCTG TGACCGATAC 
AGAGCAGATG TGTCCATTGT CCTCAGCAGC CTGAGTTACC TCTGTCATGA ACTCCATTCT 
GTCCACCAAG ACCTTATTCT TCTGCCACTT ATTTCATTAA ACCATTACTT GGTCTGTGAA 
GTTTGCCAAG ATCTATTTAA AACCATAGAT ACAAGGATCC TTAAGACGGA AATCCTTATT 
GATTTAGGAT TCTTTTCTGA AGCTTTTAGT GAAATGGCTG GTCTTTATAC TGGAAAAAAC 
ATCCCCACTT CCACGCCCTT AGGCTATAAA CTTCCAAAAA CTAAGATCAT GCAGCCCTTT 
CAAGCAGGAC ACTCTTATTT GAGCAAAGAA AATATACAGG CCCTGGAAGA TTTAATGAAT 
CAAGGCATCC CCCTATACAT TATTTCGATT TGCCAACCAT CTTTCTCCTA TAAGTTTACT 
TTGGTCAAAA TGCGCTTTTT AATCAGTTTG GCTGCAACCA TAAATTGCTT CCCAGTTAGG 
GATACAACAG CATTCTTCCA GGACTTTACC CAGAAGAGCA AAGCCAGTGC TTCAGTCTTT 
AAAGGTACAA ACCCTGAAGA TTCAATAATC CCCTCTCAAG AGACAGTTCA AAATGACTTT 
ACCGCAGCTG AAGGAACACA GAGAGCTCTG GACAAGAGCA TTGACTTCTC AAAGCAAAGA 
GGCTTCGATG ATATGAGGAA GATGCTGATG GTGGACCCAG AATATTTAAA GGATGAAACC 
CCAGCAAATG TTAACCTGAC CAAGTCAAAA GAAGAGTTGT CTCCAGGTAA ACTAAAGGGG 
ATTTTATTGG CAGAAGCTGA GGACAAGCTT CTCATGCTAA TAGAAAATGC AGAGGAATTG 
AGTCACAAGA AGATAACTCA GTTTAGCCCA ATAGAATTGG AGATTATGAT AGAAGTCAAG 
TTACAGCTAG CCGCTATTGC GATGCAGAGA CAACAAGCCC CACTGAGCAC TTCAATAGCT 
TTCACTGCAA TTAAAATCCT TCAGGATGCA GACATTTTCA AGAAGACTAA AGAAGAACTG 
TGTGCGAATG ATGATGAACT GGATGCCATG GAGTCTGTGG AGATGTTGGC CCGAGAACGT 
CTAAACATCC ATCTATGGCT AAAGTGTCGT CTGGCCTTGC TGGTGGCCAC TGTGACCCAC 
ATCCGTGGCA TGGGGGTCGT GAAAGAAAAT GAGATGATTG AGTGCAGACT TTTGATAAAT 
GAAGTATTTG TGGAGGCTCA AAACTGGAAT GATACTGAGA TGCAAGCAGA AGTCATGGTG 
CAGGCTGTCA AGTTTGACCT GCAAGAAAGA CATTCTGCAG CCAACATCTT AGAACATTTG 
AAGGATATAA GAGGCTTACT TGAAGGAAAA TCCTTTGTCT CTCCTCGAGG CTCTTTAATA 
CTTATGAAAA GCCTGCTGTT AATGGATGAT TTAATGAAAA TTGAGATGCC CCAGGAATAT 
CTCTTCCTTA AAAATGAAAA TCTCAATTTA CTAAATCGAG CTCATGAGCT TATGATTGCG 
CAGGCTCTAT CTTTGGGTGA AAACATTTTA TTGCCTTGTT CAGACACTGA ATATGCAAAT 
ATCTTCTACC CTTTGAAAAA CGTTTATTTT CCATACATCA ACTTACTTGC AAAAACAAAA 
ATGAGGATTG GGCATATGTT AGCCAAGAGC ACTGCTGCCT GTGAGAGAAA AGAACCTCAG 
AAGTGGGAAC CCATTCGTCA TCTCTTTGAG ACAGCATTGC AACTTTGTAG GGCAGCTGCA 
GTGAAGGAAC GGGAGTTAGA AGCCGAAATC CTTTTTGAGA AAGGCAAAGT AGAACGTCAA 
ATGCTTGTTG TACAAGACAA CAAGCCTTTA ATTGGTGATC TTTTTGATGC TATAAAAATA 
AGTCTACAAA ATGATGAAAA TTCAGGGTTG ATAAGGAAAT CCTATCTGGA AATCGCCCTG 
TTATATTTAC TTGTGTGGAA TTATAGGAAA AATGCTTCAA AAAATAAGAT CTTTCTCCAA 
GTCCAGAATC CATCAAAGGA ATCACTAGAA ATTAAAACTG AAATTTACCT GGCAATGTCC 
TGGATAGCAA TCCGAGCTGC TGCACAGGTC AGTGAAGCTA CAATGGCAGC TAAGAAGCTA 
ACTGGAGAAA AGAGTGTGAA GACCAAGAAA ATGGATCAAA ATGTCTATCA GAATATCCCT 
GAATTTGCTC ATGCTGACCT CACTTGTACC TATGTAGATT ACTTGACTGC TAACTATAAA 
ACTTGTGTCA AAACTTCCTA TACCACTCCG AGCGAATCTG GAAGTTATTT AAGTATGGAA 
CAAGATCTGT ACACCAACCC CAGTGAAGAA AATGAGGAGC CCAGCCAGCT GGAGATCACC 
TGGGTCCATC TGGCCCGTTA CTACACTCAC CTGTTAAGAG TTAACAATAT GAATACCTTG 
CTTGCTCCTC CAAAGCCAGT TTTCTTAACA GATGAAATTT TCTACACTTC TGTTTTCAAT 
GAAGATCTGA TTATACGTCA GAAAGAGATG AAGAATTTCC TCAAAAAATT CTTAAGTGTT 
TTTACTTCAG CCTGTCTAGA GTGTTTCCCC AAAGACCTCC TTCTTGGAAT AGAAAGACTA 
AATATTCCAG ATAATGCCGA ACTTAGGCAA TCACTTAAGA CATTTCAAGA TGTGAGTGCT 
ATAATAGAAA AAATAGTCAC CACTAAGATC AACCCCATTA TCCTTCCTCC ATCTCCCCCG 
CATGAACATA TGGATTTTGC TATACATGCA GAAAGCCCAG ATCTTTGCTT CCAGTGGTAT 
ATACCCCCTC TGGAAAAACC TCCAAGTGAT AAAGAACCCA TGGTCATGTT GCTGTATGCC 
TATAACACCA AACCTGTCAA GATTAATGAT ATCTTTGAAT TCAATATCTC CTCGATATTT 
CCTGGGTTTT TATGGCTTCC TCTGAACAAA GTTATAGCCT TACATGAAGC TCTATCTGGC 
CTTAGGCAAG AAACTGAAAT GTTGTTATAC ACAAAAAAAT TGCCTACAGA AGAAAAGAAG 
TCCCTACTAC CCTTCAGTGC CTTAAAACCT GCTAGAGAAG TCTCTGAAGA GATCGTCCTC 
AGTAAGCAAA TGAAGACTGT GTTTTATCAG AGTTGCACTG AAATAGCAAG ACTGCTTTCG 
AGAAATGAAG ACATACCCCT AATAAGTGAG GTTCCATTTG AAGAATCTTT GCCAAGTATA 
CAGAAGTTGG AGATGCTTTT TGACCCCTCC AGTGGCTCTG TCTTAAAGGC AGAAGCCATT 
TTCAACTGGA TCGTGTCTTT TCTTACATCT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007503308.1
CDS	285..8780
Translation	MNPGDMERNQWEAGDEVESKILLQWPRTATEKLLSEMFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_007503246.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007503246.2

ORF Insert Sequence:

ATGAATCCTG GAGACATGGA GAGAAATCAG TGGGAAGCTG GGGATGAAGT GGAGAGCAAG 
ATCTTGTTGC AATGGCCACG CACAGCTACA GAGAAGTTGT TGAGTGAGAT GTTTGATTGT 
GGATCAGCCC AGTTTTTGGC TGTCTTGGAA GCCCTTTCTG AACCAAATAG AAGAGTGTTA 
CAAACTGGTC ACTTGTCAGA GGAAATGGAA GTTCGTGAGG TCATCGCTGA GCTCTTCATG 
GCAGCAAGCG AGCTTTTATC AGATGCATGT AGTAGCCATG GGAAATCCAG TTTTTCCAAA 
AACATCTCTC TACAGCTTTC TCAAATGCAA CTCGCCATTG AAAGCAAACA AAGTGTTTCA 
ATAGCTGCTG CAGTGAAGTT GATAAAATTA TCTTTCACCT TTGAGGAATG GCATTTGTTT 
AATTCTGCTG CTTCTCAGTT TATCGGTTTT CTTCAGCTTC AAAGTGGCCC ACACTTGCAG 
AAAGCCAGGA AGGATTTAAT GCTTCTGGCT GCGATGGAAC CGCTAATTAA TGTGAAGCGG 
AATAAAGGCT TAAGCTATGC TGGTGAAGGA GGCAGCACTA CCACTGAAGG AGCTCGAAGC 
TCAGGGAGAA AAATTGTGTT TCAAGAAAAT TTCTCATTAT CTGGAAGCTA TTCTGATGAT 
ATTTTCTATT TGGCATCGAT GCTGTTTTCC TTCATTTGTT CACCTACTGA GAAAATTCGT 
CCTGATGAAG AGATCGTTGT TGACCTGATA ATGTTCCTGT GGCAGAAATG CAAAATGGGA 
ATTCAGAAGA TCTATTCAGC AAGAATGGAT TATTTCAAGT TTGTCCAGAA AGTCAAAACT 
AACAAATGGA TTCAGCTGCT TTGGCAGATA AATGAAATGA TTAATACTTG TCAACCAGAA 
GACCTCAACC CTGTGTTTGT AGCTGAAGTT GCATTACGAC TAAGTGAAAT ACTGGAGATT 
TTAGGAAAGC CAGAAAAAAA AAGTCCTGGC AGCGCAAGCA GAGTAACTAT AGAATTCCAG 
CAACTTCCTG ACAGTTTTCC ACTATTTAAG AAAACACCTA AAGAGCAGTT ACTTTTGGCT 
CATGAAACTC TTGAGAAGGC AGTTACTGTG ATGGGGCTAT GTCGTTCCCA AACTACATTG 
CCTAATGGAA AATCAGTATA TGACCATTGT CATATCAAGT TTATCCCTCC AAGAGGAGGT 
GGAACCACTG GCAAACCAAT CACTGACAAT ACTTTCCTGA TGGACTTGCA TCTAGAACTC 
CTTGTGAGTC AGCACCGGAT AGCTGCTACA CTCATTGATC AACTCAAAGC TTGGAATTTT 
TCTCCAAAAC CTGCCCATCC CAAAGAAACT GAAACTACAA CTACTAGTCA TTTCACAGAA 
GCATACGTAC TGGGCAAAAT CAATAAGAAT GAGTTATCCA AAGCAGTCTT CTTAATGATG 
AAAGCTCAGA TACTATTTGA GAAAGAAGAT TCTCAGGTCA TCAAATCACT GGAGGAATCG 
TTGTTCTTAA TTCGGAAGAT TGAATCAGAG CAAAAAAACC AGTATTCACC TCAGCAGAGT 
ACAGAAGAAG AAAAACCCCA GAGCAAAGTT CCTGCTCCTC CCATACTACT TTCAAGAACT 
CATTGTTCAG TGACATTTAA ACCTGATTCA TTTTTTTGTG GAGAAAAGGT CGCTTGGTAT 
GTCATTTTGG GACGTCCAGC AGGAACATGC ATGAGAAAAG TAAGATTGAA CGACCATAGT 
CTTCTAAACT GTGGAGAACC AGTACCTGCT ACTAGTAAAA CCGTTTTAGA AGTTGTTGGA 
TTGGAGCCTA ATGAAAAATA CGTGTTTGCA CTTGCTGGTT ATTGCTCTGC TGGAACACTT 
ATTGGGCAAG GAATTGGACT GACATCTAAG CCTATTCTGA TTTACATGCC ACTTTCTACT 
GTTACTATTC GCTTACATCT GACTGAGATT GCCTTTCAAC TCGGTAAATA TTCAGTGGCC 
AAGAAAGCCT TTGCACCTGT TTGGGACTAT TTCGTCGATC CTCCTCTTCC AGAAAATCCA 
CACATTTTTT CTTTAAGTAG CTTGTTAACT GTCACACCAT TCAGGTTACA TCTGGAGACC 
GTGTCAGAGT CTTCATCAAT CCTCTTATAC TATTTCATTA GATGCATTTT CATAATAAGT 
AATATTAATA TTATAGAAAA AAAGCTCTTC TGTGATACTA TTAGAGGAGA TGAGGTTTTC 
ACTTGTCAAC AAGTTTTCAG ACTACTTGAA TGTCAGAGAA TCCTTGTGGT CATTGAACTT 
AGTACCCATG TAAATAATTT TAGTTATTCC CTACAAGCTG TTGTTCACTG CTATGGTCTC 
CTGGCCCCTC TAATCTTTCA CAATATTGTC CTGGTTCCAA TAATCCAGGT CCTAATTAAG 
TGTTTGGTAA TTTTGCAAGA AATCCCAGGT GTTACCTTTC CAAAGAGACA GATGGGGAAT 
TTTGAGTCTA CCCAGCATAT GATTGCGTGC ACTGTTTATT ACACGGCAAA GGTGTTACGT 
TCATGGAAGG AATTTGACCT TGCAGTGATT ATTATAAATT TGGGGAAAAA GTTACTGGAC 
ACCTCACAAG GGTTATTTTC AAATGTCACT AAAGCTGCCT TTGGTGAGGA TGATCAAGAT 
GACTTATCAG AGGAGGCCTA CTCAACCAAG AGAATCAAGA GGGTCAAGGG ACAGATTGCC 
ATACTGGACA AAATCAGCGA GCAGCTCAAC GTCATGGAAG CTCAACTACT CAAACTGACG 
AAACAAAGTC CCATGTATGA GCTCACAGGA ACAGAAGATC CCTTTTTCCT CTACCCAATA 
ATTCTAACTT GGACTCTCAA AACTGCGATG AGGGAAGTAA TGAAATTCAA GAGTAAACCT 
CGTTTTCTGG AATTCTTTGT TCAAGTTATG ACAAAATGTC TTATTGAAGA GAAATTTCAC 
TATGTGGTGG AATTGGCATT TTCAGTTCAA CTCTTCCTTA AAAGGAGAAA TGCCAGTTTA 
CTTGGAGTAA GGAGATCTCC AGTTCTTCAC ACTCTTCCAT CTAATGAAGA ACCAAAGAAA 
TACAAGGCAG CTGCCATGGA GATTCAAAAA ACTACAGATG TATCAATTGG GAAGTCAAAA 
TCAAAGAAAA AAGGTATTCT GCAAGATTAT TTTGTCAAGC ATCTCTCACT TTCTGAATTG 
CCAGATTTGG AAAAATTTAA AAGAGCTGAT GTGAGAAAAG TAGCTATAAA AATCCTTTCA 
GAACACTTGG CCCCAAAACT AAATCGCTAT ATAAAAAGGA AAAGAATGCA TCGAATATTT 
ATTGAAGAGT TACCCTGGAA GTCTCAGATG AATGTACATT TGGCTGAAGC TCACTTCAAC 
TTATTCAAAA TCAGATTCAT GGAGCGTAAG AAGGTTAAAG CTTGCACTTC ACAAAATACA 
ATGAGCTACC GAATTTTAGA CCCGGATATC TTCTCAATGT ACAATTCTGG GACGTTGTTA 
GTGGATGAAA ACTTAAATTC AGATAATTAC CAAGCATTGC TTGATTTCAT GCTTTCAAGA 
AAAATGAGAA AGATCAATCT CTCAGATGCC AATGAAATTT CTTTTGGTGG TGTCAGATTG 
AGTGAAGATA TCCCTAAAGG GGTAAACACA AATGACCTGG AATCCGCTAG AGAGAAAACA 
GCAAATCTTG GAATCATAGA CCATTTTATC AGGACCTTTG TTCACTGCAA AAGAGCATTG 
GTTCTTGCTC ATCGTGGTGG TTTCTGGACC TTACTTCAGA ATGTGACCCG GACTCTTTGG 
AATTACACCA TAGAGATGCA GTTACTCTAT AAACAGGCGC AGTTTCTTTC CAAAACATTC 
CCTTTCAGCA AGGATATTAT GATATGCACC TTCGTGATGC CATTTACTTT GGGAGCAGAT 
GCCCTTATTG ACATGCTGTT AGACCTGCAG AATACCAAAT CTCTTAAGAT TGTGGATGAC 
TGTAAAGATT TTGGTGTTGC AAGTGCTTAT GGGAACATCA CAGAGGATAA TGGTGGCTTC 
AATCTGACAT TTGAAGAACC TTTTGATGAT ATGAATTTGG TTGACTTGAA ATTCATTTCT 
GACTTCATCT TTAAGACTTT GGAAATCTTG TACAGAGTGG GAAAATGGGA AACACTAGTC 
TACATTGCCC TTCAGTTCAA TGAAGTGACA CATGAGAGAT ACACTGAGCA AGTGATGCCA 
TTGTTAATCT ATTCTCAAAG ACAGCTTTTG CAGAGACTCC AGAGAAATAG TGGGGAGCAG 
ACAGATAATA TAGAATGGCA GTTTGATGAC ACGGTAAACC TCCGAGATGT CATTGGGAAA 
CTGCCCAATT CTTCTTTTGT GCAGACTATA AAGGGCAAGT CTAAAAGACT TTTTAATTAT 
GAGCAAGGTG TAGATCTTTC GGAGACATAC ATTGCAAATA GAATAGTGTC TGTGCCCCTG 
AATGTGAAAG AAACTTTGAA GACCTTTAGA GAAACCTTGA CGAAATCTAA GTACCACAAC 
AGATCACTGA GATACAGCAG AAAATTGCTT TCTTTGTTAC TGGCCCACAC ACAAGAAGCC 
AGTGGAGGAA TAAACAATCC CAAATTTATG TATGGAAAAG TGGAATTTAG CCTTGGAACT 
GAAGAAATAC ATTCGCCTAC ACCCCCTGAT CTTTTTAATG AAAAGTTCAC TGCTTCTGTT 
ACAGTACAGA GCACAAAAAT TTCTCCTTTC CAATTTCAAC TTGTTATCAC TTCTTATGAG 
AAAACGATCG AGATTCTTGA AATCAGCAAT CAAACAGATC TCAAGATACA GGCACTACAT 
GAGCTTGGAA ATCTTCACAT CTATGCAGAA AACAGAAGGG CAGCTTTTAA ATGCTGGAGT 
CAAGCTCTTG ATGCCATACT CAAAAAAAAA GATGTTCTAC ATACGTGGAA AGAAATTGTC 
AGCCCCATCC AAAAACCTAT GAATAGCATC GTGGGTTCAA ACTACATCGA TTATAGTGAA 
GATTTTCTGT TGAAAGCTGG CATTTGGGGA TGCTTGCAGG GAGGAGTCAT AGCAGCAAAG 
ATGGCCCAAT ACATTTTAAC ATCAAATATG AATGAGAGGA TTGAATGTTG CATTTTCTCT 
TCATTTTTTT TTAAGGGTCT ACTTAGAACC TCTCTTGTCC ATCCCAAATA TGACCATGAG 
TATGCCCGTT ATGAAATCAC CCAACTTACT CCAGGGCTGG ATCTCTTCTG TGACCGATAC 
AGAGCAGATG TGTCCATTGT CCTCAGCAGC CTGAGTTACC TCTGTCATGA ACTCCATTCT 
GTCCACCAAG ACCTTATTCT TCTGCCACTT ATTTCATTAA ACCATTACTT GGTCTGTGAA 
GTTTGCCAAG ATCTATTTAA AACCATAGAT ACAAGGATCC TTAAGACGGA AATCCTTATT 
GATTTAGGAT TCTTTTCTGA AGCTTTTAGT GAAATGGCTG GTCTTTATAC TGGAAAAAAC 
ATCCCCACTT CCACGCCCTT AGGCTATAAA CTTCCAAAAA CTAAGATCAT GCAGCCCTTT 
CAAGCAGGAC ACTCTTATTT GAGCAAAGAA AATATACAGG CCCTGGAAGA TTTAATGAAT 
CAAGGCATCC CCCTATACAT TATTTCGATT TGCCAACCAT CTTTCTCCTA TAAGTTTACT 
TTGGTCAAAA TGCGCTTTTT AATCAGTTTG GCTGCAACCA TAAATTGCTT CCCAGTTAGG 
GATACAACAG CATTCTTCCA GGACTTTACC CAGAAGAGCA AAGCCAGTGC TTCAGTCTTT 
AAAGGTACAA ACCCTGAAGA TTCAATAATC CCCTCTCAAG AGACAGTTCA AAATGACTTT 
ACCGCAGCTG AAGGAACACA GAGAGCTCTG GACAAGAGCA TTGACTTCTC AAAGCAAAGA 
GGCTTCGATG ATATGAGGAA GATGCTGATG GTGGACCCAG AATATTTAAA GGATGAAACC 
CCAGCAAATG TTAACCTGAC CAAGTCAAAA GAAGAGTTGT CTCCAGGTAA ACTAAAGGGG 
ATTTTATTGG CAGAAGCTGA GGACAAGCTT CTCATGCTAA TAGAAAATGC AGAGGAATTG 
AGTCACAAGA AGATAACTCA GTTTAGCCCA ATAGAATTGG AGATTATGAT AGAAGTCAAG 
TTACAGCTAG CCGCTATTGC GATGCAGAGA CAACAAGCCC CACTGAGCAC TTCAATAGCT 
TTCACTGCAA TTAAAATCCT TCAGGATGCA GACATTTTCA AGAAGACTAA AGAAGAACTG 
TGTGCGAATG ATGATGAACT GGATGCCATG GAGTCTGTGG AGATGTTGGC CCGAGAACGT 
CTAAACATCC ATCTATGGCT AAAGTGTCGT CTGGCCTTGC TGGTGGCCAC TGTGACCCAC 
ATCCGTGGCA TGGGGGTCGT GAAAGAAAAT GAGATGATTG AGTGCAGACT TTTGATAAAT 
GAAGTATTTG TGGAGGCTCA AAACTGGAAT GATACTGAGA TGCAAGCAGA AGTCATGGTG 
CAGGCTGTCA AGTTTGACCT GCAAGAAAGA CATTCTGCAG CCAACATCTT AGAACATTTG 
AAGGATATAA GAGGCTTACT TGAAGGAAAA TCCTTTGTCT CTCCTCGAGG CTCTTTAATA 
CTTATGAAAA GCCTGCTGTT AATGGATGAT TTAATGAAAA TTGAGATGCC CCAGGAATAT 
CTCTTCCTTA AAAATGAAAA TCTCAATTTA CTAAATCGAG CTCATGAGCT TATGATTGCG 
CAGGCTCTAT CTTTGGGTGA AAACATTTTA TTGCCTTGTT CAGACACTGA ATATGCAAAT 
ATCTTCTACC CTTTGAAAAA CGTTTATTTT CCATACATCA ACTTACTTGC AAAAACAAAA 
ATGAGGATTG GGCATATGTT AGCCAAGAGC ACTGCTGCCT GTGAGAGAAA AGAACCTCAG 
AAGTGGGAAC CCATTCGTCA TCTCTTTGAG ACAGCATTGC AACTTTGTAG GGCAGCTGCA 
GTGAAGGAAC GGGAGTTAGA AGCCGAAATC CTTTTTGAGA AAGGCAAAGT AGAACGTCAA 
ATGCTTGTTG TACAAGACAA CAAGCCTTTA ATTGGTGATC TTTTTGATGC TATAAAAATA 
AGTCTACAAA ATGATGAAAA TTCAGGGTTG ATAAGGAAAT CCTATCTGGA AATCGCCCTG 
TTATATTTAC TTGTGTGGAA TTATAGGAAA AATGCTTCAA AAAATAAGAT CTTTCTCCAA 
GTCCAGAATC CATCAAAGGA ATCACTAGAA ATTAAAACTG AAATTTACCT GGCAATGTCC 
TGGATAGCAA TCCGAGCTGC TGCACAGGTC AGTGAAGCTA CAATGGCAGC TAAGAAGCTA 
ACTGGAGAAA AGAGTGTGAA GACCAAGAAA ATGGATCAAA ATGTCTATCA GAATATCCCT 
GAATTTGCTC ATGCTGACCT CACTTGTACC TATGTAGATT ACTTGACTGC TAACTATAAA 
ACTTGTGTCA AAACTTCCTA TACCACTCCG AGCGAATCTG GAAGTTATTT AAGTATGGAA 
CAAGATCTGT ACACCAACCC CAGTGAAGAA AATGAGGAGC CCAGCCAGCT GGAGATCACC 
TGGGTCCATC TGGCCCGTTA CTACACTCAC CTGTTAAGAG TTAACAATAT GAATACCTTG 
CTTGCTCCTC CAAAGCCAGT TTTCTTAACA GATGAAATTT TCTACACTTC TGTTTTCAAT 
GAAGATCTGA TTATACGTCA GAAAGAGATG AAGAATTTCC TCAAAAAATT CTTAAGTGTT 
TTTACTTCAG CCTGTCTAGA GTGTTTCCCC AAAGACCTCC TTCTTGGAAT AGAAAGACTA 
AATATTCCAG ATAATGCCGA ACTTAGGCAA TCACTTAAGA CATTTCAAGA TGTGAGTGCT 
ATAATAGAAA AAATAGTCAC CACTAAGATC AACCCCATTA TCCTTCCTCC ATCTCCCCCG 
CATGAACATA TGGATTTTGC TATACATGCA GAAAGCCCAG ATCTTTGCTT CCAGTGGTAT 
ATACCCCCTC TGGAAAAACC TCCAAGTGAT AAAGAACCCA TGGTCATGTT GCTGTATGCC 
TATAACACCA AACCTGTCAA GATTAATGAT ATCTTTGAAT TCAATATCTC CTCGATATTT 
CCTGGGTTTT TATGGCTTCC TCTGAACAAA GTTATAGCCT TACATGAAGC TCTATCTGGC 
CTTAGGCAAG AAACTGAAAT GTTGTTATAC ACAAAAAAAT TGCCTACAGA AGAAAAGAAG 
TCCCTACTAC CCTTCAGTGC CTTAAAACCT GCTAGAGAAG TCTCTGAAGA GATCGTCCTC 
AGTAAGCAAA TGAAGACTGT GTTTTATCAG AGTTGCACTG AAATAGCAAG ACTGCTTTCG 
AGAAATGAAG ACATACCCCT AATAAGTGAG GTTCCATTTG AAGAATCTTT GCCAAGTATA 
CAGAAGTTGG AGATGCTTTT TGACCCCTCC AGTGGCTCTG TCTTAAAGGC AGAAGCCATT 
TTCAACTGGA TCGTGTCTTT TCTTACATCT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP54 ( XM_016425734.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281220.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA.

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CloneID	OMc45043
Clone ID Related Accession (Same CDS sequence)	XM_016425734.1
Accession Version	XM_016425734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGG GGATTCATGT AGGGGCACTG TGCATGGGTG AGCAGAGGAA GCGAAACAGG 
AGAGGAAGAG GAACAGAAGG GGGAAGAGGG AGAACAAAGA GAAGAGGATG GAAGAAGATA 
GGAGAAAAGT GTTGGAGTAC CTTCACCTTG TTCAATATCT GGTTCAAGTA TCAGAACCGC 
CTGCCAGTCC AGTATTACCA TGAGCATCTT TTGAAGACTG GAGACACCCT TATTCAAGCA 
AAAGAATACA AGTTGGCCTT AATACAATGC TATGGGAAGT ATCTTAGTCA GTTCAATCCC 
AGTCACGATG AGAGTGACAT GAACGTGACA ACTTTCAAAA AAGTCTTTTT CTCCAAAGGT 
GTCAATGATG AAAGCGCCAA ACTTACATTT CACGCTTTGC AAGGGAAATG TATTAGCCAG 
TACCAAATTA TCTGTGCCAC CGATCCCTGT TTCAAGAGGG AGCTGTCTGT GGAGCAGTGC 
TTCAAGATTC TGTCGGTTTT TCGGCTCATG ATGCAGATAA CCCTGCCCTA CGAACACCTT 
TGCTGGATCG TGTACAATGG TACAATTCAC ATTTATACAA TTTGCAGGAA GCTGATGACC 
ATTGGACAGT CTGCTAAGGC CTTGGAATTC TTGCTCTGGG CCAGTATTTG TATGGAATCC 
TCTTTACCCC TCTTAGCAGT AAGCTATCTC AGTTGGAGAG CTACCCTGTA TACTGCTGTT 
TGCCAGTGCT ACTATGACTG CCATGCTGGA ATGCAGGGAG AGATATTTGC TCGGCGAGGT 
TTGGGAAAAA TTGATGATTT ATTACGTCTA GAAGTAATGA GTATCACACC ACCACAGGAG 
GAAGAAAGAA AAAAATTCCG AGAGGCCACC ATCAAGATGG CGGTGATGAT CTTTAAGAGA 
GGAGTCTTTG AACCACGAAG AAAGAGTAAG TCTTATGTAA GAACAAAACT GAGACTGAAC 
CTACGGGAAG CACAGAATTT GCAATGGCCA CGCACAGCTA CAGAGAAGTT GTTGAGTGAG 
ATGTTTGATT GTGGATCAGC CCAGTTTTTG GCTGTCTTGG AAGCCCTTTC TGAACCAAAT 
AGAAGAGTGT TACAAACTGG TCACTTGTCA GAGGAAATGG AAGTTCGTGA GGTCATCGCT 
GAGCTCTTCA TGGCAGCAAG CGAGCTTTTA TCAGATGCAT GTAGTAGCCA TGGGAAATCC 
AGTTTTTCCA AAAACATCTC TCTACAGCTT TCTCAAATGC AACTCGCCAT TGAAAGCAAA 
CAAAGTGTTT CAATAGCTGC TGCAGTGAAG TTGATAAAAT TATCTTTCAC CTTTGAGGAA 
TGGCATTTGT TTAATTCTGC TGCTTCTCAG TTTATCGGTT TTCTTCAGCT TCAAAGTGGC 
CCACACTTGC AGAAAGCCAG GAAGGATTTA ATGCTTCTGG CTGCGATGGA ACCGCTAATT 
AATGTGAAGC GGAATAAAGG CTTAAGCTAT GCTGGTGAAG GAGGCAGCAC TACCACTGAA 
GGAGCTCGAA GCTCAGGGAG AAAAATTGTG TTTCAAGAAA ATTTCTCATT ATCTGGAAGC 
TATTCTGATG ATATTTTCTA TTTGGCATCG ATGCTGTTTT CCTTCATTTG TTCACCTACT 
GAGAAAATTC GTCCTGATGA AGAGATCGTT GTTGACCTGA TAATGTTCCT GTGGCAGAAA 
TGCAAAATGG GAATTCAGAA GATCTATTCA GCAAGAATGG ATTATTTCAA GTTTGTCCAG 
AAAGTCAAAA CTAACAAATG GATTCAGCTG CTTTGGCAGA TAAATGAAAT GATTAATACT 
TGTCAACCAG AAGACCTCAA CCCTGTGTTT GTAGCTGAAG TTGCATTACG ACTAAGTGAA 
ATACTGGAGA TTTTAGGAAA GCCAGAAAAA AAAAGTCCTG GCAGCGCAAG CAGAGTAACT 
ATAGAATTCC AGCAACTTCC TGACAGTTTT CCACTATTTA AGAAAACACC TAAAGAGCAG 
TTACTTTTGG CTCATGAAAC TCTTGAGAAG GCAGTTACTG TGATGGGGCT ATGTCGTTCC 
CAAACTACAT TGCCTAATGG AAAATCAGTA TATGACCATT GTCATATCAA GTTTATCCCT 
CCAAGAGGAG GTGGAACCAC TGGCAAACCA ATCACTGACA ATACTTTCCT GATGGACTTG 
CATCTAGAAC TCCTTGTGAG TCAGCACCGG ATAGCTGCTA CACTCATTGA TCAACTCAAA 
GCTTGGAATT TTTCTCCAAA ACCTGCCCAT CCCAAAGAAA CTGAAACTAC AACTACTAGT 
CATTTCACAG AAGCATACGT ACTGGGCAAA ATCAATAAGA ATGAGTTATC CAAAGCAGTC 
TTCTTAATGA TGAAAGCTCA GATACTATTT GAGAAAGAAG ATTCTCAGGT CATCAAATCA 
CTGGAGGAAT CGTTGTTCTT AATTCGGAAG ATTGAATCAG AGCAAAAAAA CCAGTATTCA 
CCTCAGCAGA GTACAGAAGA AGAAAAACCC CAGAGCAAAG TTCCTGCTCC TCCCATACTA 
CTTTCAAGAA CTCATTGTTC AGTGACATTT AAACCTGATT CATTTTTTTG TGGAGAAAAG 
GTCGCTTGGT ATGTCATTTT GGGACGTCCA GCAGGAACAT GCATGAGAAA AGTAAGATTG 
AACGACCATA GTCTTCTAAA CTGTGGAGAA CCAGTACCTG CTACTAGTAA AACCGTTTTA 
GAAGTTGTTG GATTGGAGCC TAATGAAAAA TACGTGTTTG CACTTGCTGG TTATTGCTCT 
GCTGGAACAC TTATTGGGCA AGGAATTGGA CTGACATCTA AGCCTATTCT GATTTACATG 
CCACTTTCTA CTGTTACTAT TCGCTTACAT CTGACTGAGA TTGCCTTTCA ACTCGGTAAA 
TATTCAGTGG CCAAGAAAGC CTTTGCACCT GTTTGGGACT ATTTCGTCGA TCCTCCTCTT 
CCAGAAAATC CACACATTTT TTCTTTAAGT AGCTTGTTAA CTGTCACACC ATTCAGGTTA 
CATCTGGAGA CCGTGTCAGA GTCTTCATCA ATCCTCTTAT ACTATTTCAT TAGATGCATT 
TTCATAATAA GTAATATTAA TATTATAGAA AAAAAGCTCT TCTGTGATAC TATTAGAGGA 
GATGAGGTTT TCACTTGTCA ACAAGTTTTC AGACTACTTG AATGTCAGAG AATCCTTGTG 
GTCATTGAAC TTAGTACCCA TGTAAATAAT TTTAGTTATT CCCTACAAGC TGTTGTTCAC 
TGCTATGGTC TCCTGGCCCC TCTAATCTTT CACAATATTG TCCTGGTTCC AATAATCCAG 
GTCCTAATTA AGTGTTTGGT AATTTTGCAA GAAATCCCAG GTGTTACCTT TCCAAAGAGA 
CAGATGGGGA ATTTTGAGTC TACCCAGCAT ATGATTGCGT GCACTGTTTA TTACACGGCA 
AAGGTGTTAC GTTCATGGAA GGAATTTGAC CTTGCAGTGA TTATTATAAA TTTGGGGAAA 
AAGTTACTGG ACACCTCACA AGGGTTATTT TCAAATGTCA CTAAAGCTGC CTTTGGTGAG 
GATGATCAAG ATGACTTATC AGAGGAGGCC TACTCAACCA AGAGAATCAA GAGGGTCAAG 
GGACAGATTG CCATACTGGA CAAAATCAGC GAGCAGCTCA ACGTCATGGA AGCTCAACTA 
CTCAAACTGA CGAAACAAAG TCCCATGTAT GAGCTCACAG GAACAGAAGA TCCCTTTTTC 
CTCTACCCAA TAATTCTAAC TTGGACTCTC AAAACTGCGA TGAGGGAAGT AATGAAATTC 
AAGAGTAAAC CTCGTTTTCT GGAATTCTTT GTTCAAGTTA TGACAAAATG TCTTATTGAA 
GAGAAATTTC ACTATGTGGT GGAATTGGCA TTTTCAGTTC AACTCTTCCT TAAAAGGAGA 
AATGCCAGTT TACTTGGAGT AAGGAGATCT CCAGTTCTTC ACACTCTTCC ATCTAATGAA 
GAACCAAAGA AATACAAGGC AGCTGCCATG GAGATTCAAA AAACTACAGA TGTATCAATT 
GGGAAGTCAA AATCAAAGAA AAAAGGTATT CTGCAAGATT ATTTTGTCAA GCATCTCTCA 
CTTTCTGAAT TGCCAGATTT GGAAAAATTT AAAAGAGCTG ATGTGAGAAA AGTAGCTATA 
AAAATCCTTT CAGAACACTT GGCCCCAAAA CTAAATCGCT ATATAAAAAG GAAAAGAATG 
CATCGAATAT TTATTGAAGA GTTACCCTGG AAGTCTCAGA TGAATGTACA TTTGGCTGAA 
GCTCACTTCA ACTTATTCAA AATCAGATTC ATGGAGCGTA AGAAGGTTAA AGCTTGCACT 
TCACAAAATA CAATGAGCTA CCGAATTTTA GACCCGGATA TCTTCTCAAT GTACAATTCT 
GGGACGTTGT TAGTGGATGA AAACTTAAAT TCAGATAATT ACCAAGCATT GCTTGATTTC 
ATGCTTTCAA GAAAAATGAG AAAGATCAAT CTCTCAGATG CCAATGAAAT TTCTTTTGGT 
GGTGTCAGAT TGAGTGAAGA TATCCCTAAA GGGGTAAACA CAAATGACCT GGAATCCGCT 
AGAGAGAAAA CAGCAAATCT TGGAATCATA GACCATTTTA TCAGGACCTT TGTTCACTGC 
AAAAGAGCAT TGGTTCTTGC TCATCGTGGT GGTTTCTGGA CCTTACTTCA GAATGTGACC 
CGGACTCTTT GGAATTACAC CATAGAGATG CAGTTACTCT ATAAACAGGC GCAGTTTCTT 
TCCAAAACAT TCCCTTTCAG CAAGGATATT ATGATATGCA CCTTCGTGAT GCCATTTACT 
TTGGGAGCAG ATGCCCTTAT TGACATGCTG TTAGACCTGC AGAATACCAA ATCTCTTAAG 
ATTGTGGATG ACTGTAAAGA TTTTGGTGTT GCAAGTGCTT ATGGGAACAT CACAGAGGAT 
AATGGTGGCT TCAATCTGAC ATTTGAAGAA CCTTTTGATG ATATGAATTT GGTTGACTTG 
AAATTCATTT CTGACTTCAT CTTTAAGACT TTGGAAATCT TGTACAGAGT GGGAAAATGG 
GAAACACTAG TCTACATTGC CCTTCAGTTC AATGAAGTGA CACATGAGAG ATACACTGAG 
CAAGTGATGC CATTGTTAAT CTATTCTCAA AGACAGCTTT TGCAGAGACT CCAGAGAAAT 
AGTGGGGAGC AGACAGATAA TATAGAATGG CAGTTTGATG ACACGGTAAA CCTCCGAGAT 
GTCATTGGGA AACTGCCCAA TTCTTCTTTT GTGCAGACTA TAAAGGGCAA GTCTAAAAGA 
CTTTTTAATT ATGAGCAAGG TGTAGATCTT TCGGAGACAT ACATTGCAAA TAGAATAGTG 
TCTGTGCCCC TGAATGTGAA AGAAACTTTG AAGACCTTTA GAGAAACCTT GACGAAATCT 
AAGTACCACA ACAGATCACT GAGATACAGC AGAAAATTGC TTTCTTTGTT ACTGGCCCAC 
ACACAAGAAG CCAGTGGAGG AATAAACAAT CCCAAATTTA TGTATGGAAA AGTGGAATTT 
AGCCTTGGAA CTGAAGAAAT ACATTCGCCT ACACCCCCTG ATCTTTTTAA TGAAAAGTTC 
ACTGCTTCTG TTACAGTACA GAGCACAAAA ATTTCTCCTT TCCAATTTCA ACTTGTTATC 
ACTTCTTATG AGAAAACGAT CGAGATTCTT GAAATCAGCA ATCAAACAGA TCTCAAGATA 
CAGGCACTAC ATGAGCTTGG AAATCTTCAC ATCTATGCAG AAAACAGAAG GGCAGCTTTT 
AAATGCTGGA GTCAAGCTCT TGATGCCATA CTCAAAAAAA AAGATGTTCT ACATACGTGG 
AAAGAAATTG TCAGCCCCAT CCAAAAACCT ATGAATAGCA TCGTGGGTTC AAACTACATC 
GATTATAGTG AAGATTTTCT GTTGAAAGCT GGCATTTGGG GATGCTTGCA GGGAGGAGTC 
ATAGCAGCAA AGATGGCCCA ATACATTTTA ACATCAAATA TGAATGAGAG GATTGAATGT 
TGCATTTTCT CTTCATTTTT TTTTAAGGGT CTACTTAGAA CCTCTCTTGT CCATCCCAAA 
TATGACCATG AGTATGCCCG TTATGAAATC ACCCAACTTA CTCCAGGGCT GGATCTCTTC 
TGTGACCGAT ACAGAGCAGA TGTGTCCATT GTCCTCAGCA GCCTGAGTTA CCTCTGTCAT 
GAACTCCATT CTGTCCACCA AGACCTTATT CTTCTGCCAC TTATTTCATT AAACCATTAC 
TTGGTCTGTG AAGTTTGCCA AGATCTATTT AAAACCATAG ATACAAGGAT CCTTAAGACG 
GAAATCCTTA TTGATTTAGG ATTCTTTTCT GAAGCTTTTA GTGAAATGGC TGGTCTTTAT 
ACTGGAAAAA ACATCCCCAC TTCCACGCCC TTAGGCTATA AACTTCCAAA AACTAAGATC 
ATGCAGCCCT TTCAAGCAGG ACACTCTTAT TTGAGCAAAG AAAATATACA GGCCCTGGAA 
GATTTAATGA ATCAAGGCAT CCCCCTATAC ATTATTTCGA TTTGCCAACC ATCTTTCTCC 
TATAAGTTTA CTTTGGTCAA AATGCGCTTT TTAATCAGTT TGGCTGCAAC CATAAATTGC 
TTCCCAGTTA GGGATACAAC AGCATTCTTC CAGGACTTTA CCCAGAAGAG CAAAGCCAGT 
GCTTCAGTCT TTAAAGGTAC AAACCCTGAA GATTCAATAA TCCCCTCTCA AGAGACAGTT 
CAAAATGACT TTACCGCAGC TGAAGGAACA CAGAGAGCTC TGGACAAGAG CATTGACTTC 
TCAAAGCAAA GAGGCTTCGA TGATATGAGG AAGATGCTGA TGGTGGACCC AGAATATTTA 
AAGGATGAAA CCCCAGCAAA TGTTAACCTG ACCAAGTCAA AAGAAGAGTT GTCTCCAGGT 
AAACTAAAGG GGATTTTATT GGCAGAAGCT GAGGACAAGC TTCTCATGCT AATAGAAAAT 
GCAGAGGAAT TGAGTCACAA GAAGATAACT CAGTTTAGCC CAATAGAATT GGAGATTATG 
ATAGAAGTCA AGTTACAGCT AGCCGCTATT GCGATGCAGA GACAACAAGC CCCACTGAGC 
ACTTCAATAG CTTTCACTGC AATTAAAATC CTTCAGGATG CAGACATTTT CAAGAAGACT 
AAAGAAGAAC TGTGTGCGAA TGATGATGAA CTGGATGCCA TGGAGTCTGT GGAGATGTTG 
GCCCGAGAAC GTCTAAACAT CCATCTATGG CTAAAGTGTC GTCTGGCCTT GCTGGTGGCC 
ACTGTGACCC ACATCCGTGG CATGGGGGTC GTGAAAGAAA ATGAGATGAT TGAGTGCAGA 
CTTTTGATAA ATGAAGTATT TGTGGAGGCT CAAAACTGGA ATGATACTGA GATGCAAGCA 
GAAGTCATGG TGCAGGCTGT CAAGTTTGAC CTGCAAGAAA GACATTCTGC AGCCAACATC 
TTAGAACATT TGAAGGATAT AAGAGGCTTA CTTGAAGGAA AATCCTTTGT CTCTCCTCGA 
GGCTCTTTAA TACTTATGAA AAGCCTGCTG TTAATGGATG ATTTAATGAA AATTGAGATG 
CCCCAGGAAT ATCTCTTCCT TAAAAATGAA AATCTCAATT TACTAAATCG AGCTCATGAG 
CTTATGATTG CGCAGGCTCT ATCTTTGGGT GAAAACATTT TATTGCCTTG TTCAGACACT 
GAATATGCAA ATATCTTCTA CCCTTTGAAA AACGTTTATT TTCCATACAT CAACTTACTT 
GCAAAAACAA AAATGAGGAT TGGGCATATG TTAGCCAAGA GCACTGCTGC CTGTGAGAGA 
AAAGAACCTC AGAAGTGGGA ACCCATTCGT CATCTCTTTG AGACAGCATT GCAACTTTGT 
AGGGCAGCTG CAGTGAAGGA ACGGGAGTTA GAAGCCGAAA TCCTTTTTGA GAAAGGCAAA 
GTAGAACGTC AAATGCTTGT TGTACAAGAC AACAAGCCTT TAATTGGTGA TCTTTTTGAT 
GCTATAAAAA TAAGTCTACA AAATGATGAA AATTCAGGGT TGATAAGGAA ATCCTATCTG 
GAAATCGCCC TGTTATATTT ACTTGTGTGG AATTATAGGA AAAATGCTTC AAAAAATAAG 
ATCTTTCTCC AAGTCCAGAA TCCATCAAAG GAATCACTAG AAATTAAAAC TGAAATTTAC 
CTGGCAATGT CCTGGATAGC AATCCGAGCT GCTGCACAGG TCAGTGAAGC TACAATGGCA 
GCTAAGAAGC TAACTGGAGA AAAGAGTGTG AAGACCAAGA AAATGGATCA AAATGTCTAT 
CAGAATATCC CTGAATTTGC TCATGCTGAC CTCACTTGTA CCTATGTAGA TTACTTGACT 
GCTAACTATA AAACTTGTGT CAAAACTTCC TATACCACTC CGAGCGAATC TGGAAGTTAT 
TTAAGTATGG AACAAGATCT GTACACCAAC CCCAGTGAAG AAAATGAGGA GCCCAGCCAG 
CTGGAGATCA CCTGGGTCCA TCTGGCCCGT TACTACACTC ACCTGTTAAG AGTTAACAAT 
ATGAATACCT TGCTTGCTCC TCCAAAGCCA GTTTTCTTAA CAGATGAAAT TTTCTACACT 
TCTGTTTTCA ATGAAGATCT GATTATACGT CAGAAAGAGA TGAAGAATTT CCTCAAAAAA 
TTCTTAAGTG TTTTTACTTC AGCCTGTCTA GAGTGTTTCC CCAAAGACCT CCTTCTTGGA 
ATAGAAAGAC TAAATATTCC AGATAATGCC GAACTTAGGC AATCACTTAA GACATTTCAA 
GATGTGAGTG CTATAATAGA AAAAATAGTC ACCACTAAGA TCAACCCCAT TATCCTTCCT 
CCATCTCCCC CGCATGAACA TATGGATTTT GCTATACATG CAGAAAGCCC AGATCTTTGC 
TTCCAGTGGT ATATACCCCC TCTGGAAAAA CCTCCAAGTG ATAAAGAACC CATGGTCATG 
TTGCTGTATG CCTATAACAC CAAACCTGTC AAGATTAATG ATATCTTTGA ATTCAATATC 
TCCTCGATAT TTCCTGGGTT TTTATGGCTT CCTCTGAACA AAGTTATAGC CTTACATGAA 
GCTCTATCTG GCCTTAGGCA AGAAACTGAA ATGTTGTTAT ACACAAAAAA ATTGCCTACA 
GAAGAAAAGA AGTCCCTACT ACCCTTCAGT GCCTTAAAAC CTGCTAGAGA AGTCTCTGAA 
GAGATCGTCC TCAGTAAGCA AATGAAGACT GTGTTTTATC AGAGTTGCAC TGAAATAGCA 
AGACTGCTTT CGAGAAATGA AGACATACCC CTAATAAGTG AGGTTCCATT TGAAGAATCT 
TTGCCAAGTA TACAGAAGTT GGAGATGCTT TTTGACCCCT CCAGTGGCTC TGTCTTAAAG 
GCAGAAGCCA TTTTCAACTG GATCGTGTCT TTTCTTACAT CTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281220.1
CDS	16..9423
Translation	MGAGIHVGALCMGEQRKRNRRGRGTEGGRGRTKRRGWKKIGEKCWSTFTLFNIWFKYQNRLPVQYYHEHLLKTGDTLIQAKEYKLALIQCYGKYLSQFNPSHDESDMNVTTFKKVFFSKGVNDESAKLTFHALQGKCISQYQIICATDPCFKRELSVEQCFKILSVFRLMMQITLPYEHLCWIVYNGTIHIYTICRKLMTIGQSAKALEFLLWASICMESSLPLLAVSYLSWRATLYTAVCQCYYDCHAGMQGEIFARRGLGKIDDLLRLEVMSITPPQEEERKKFREATIKMAVMIFKRGVFEPRRKSKSYVRTKLRLNLREAQNLQWPRTATEKLLSEMFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_016425734.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425734.1

ORF Insert Sequence:

ATGGGGGCGG GGATTCATGT AGGGGCACTG TGCATGGGTG AGCAGAGGAA GCGAAACAGG 
AGAGGAAGAG GAACAGAAGG GGGAAGAGGG AGAACAAAGA GAAGAGGATG GAAGAAGATA 
GGAGAAAAGT GTTGGAGTAC CTTCACCTTG TTCAATATCT GGTTCAAGTA TCAGAACCGC 
CTGCCAGTCC AGTATTACCA TGAGCATCTT TTGAAGACTG GAGACACCCT TATTCAAGCA 
AAAGAATACA AGTTGGCCTT AATACAATGC TATGGGAAGT ATCTTAGTCA GTTCAATCCC 
AGTCACGATG AGAGTGACAT GAACGTGACA ACTTTCAAAA AAGTCTTTTT CTCCAAAGGT 
GTCAATGATG AAAGCGCCAA ACTTACATTT CACGCTTTGC AAGGGAAATG TATTAGCCAG 
TACCAAATTA TCTGTGCCAC CGATCCCTGT TTCAAGAGGG AGCTGTCTGT GGAGCAGTGC 
TTCAAGATTC TGTCGGTTTT TCGGCTCATG ATGCAGATAA CCCTGCCCTA CGAACACCTT 
TGCTGGATCG TGTACAATGG TACAATTCAC ATTTATACAA TTTGCAGGAA GCTGATGACC 
ATTGGACAGT CTGCTAAGGC CTTGGAATTC TTGCTCTGGG CCAGTATTTG TATGGAATCC 
TCTTTACCCC TCTTAGCAGT AAGCTATCTC AGTTGGAGAG CTACCCTGTA TACTGCTGTT 
TGCCAGTGCT ACTATGACTG CCATGCTGGA ATGCAGGGAG AGATATTTGC TCGGCGAGGT 
TTGGGAAAAA TTGATGATTT ATTACGTCTA GAAGTAATGA GTATCACACC ACCACAGGAG 
GAAGAAAGAA AAAAATTCCG AGAGGCCACC ATCAAGATGG CGGTGATGAT CTTTAAGAGA 
GGAGTCTTTG AACCACGAAG AAAGAGTAAG TCTTATGTAA GAACAAAACT GAGACTGAAC 
CTACGGGAAG CACAGAATTT GCAATGGCCA CGCACAGCTA CAGAGAAGTT GTTGAGTGAG 
ATGTTTGATT GTGGATCAGC CCAGTTTTTG GCTGTCTTGG AAGCCCTTTC TGAACCAAAT 
AGAAGAGTGT TACAAACTGG TCACTTGTCA GAGGAAATGG AAGTTCGTGA GGTCATCGCT 
GAGCTCTTCA TGGCAGCAAG CGAGCTTTTA TCAGATGCAT GTAGTAGCCA TGGGAAATCC 
AGTTTTTCCA AAAACATCTC TCTACAGCTT TCTCAAATGC AACTCGCCAT TGAAAGCAAA 
CAAAGTGTTT CAATAGCTGC TGCAGTGAAG TTGATAAAAT TATCTTTCAC CTTTGAGGAA 
TGGCATTTGT TTAATTCTGC TGCTTCTCAG TTTATCGGTT TTCTTCAGCT TCAAAGTGGC 
CCACACTTGC AGAAAGCCAG GAAGGATTTA ATGCTTCTGG CTGCGATGGA ACCGCTAATT 
AATGTGAAGC GGAATAAAGG CTTAAGCTAT GCTGGTGAAG GAGGCAGCAC TACCACTGAA 
GGAGCTCGAA GCTCAGGGAG AAAAATTGTG TTTCAAGAAA ATTTCTCATT ATCTGGAAGC 
TATTCTGATG ATATTTTCTA TTTGGCATCG ATGCTGTTTT CCTTCATTTG TTCACCTACT 
GAGAAAATTC GTCCTGATGA AGAGATCGTT GTTGACCTGA TAATGTTCCT GTGGCAGAAA 
TGCAAAATGG GAATTCAGAA GATCTATTCA GCAAGAATGG ATTATTTCAA GTTTGTCCAG 
AAAGTCAAAA CTAACAAATG GATTCAGCTG CTTTGGCAGA TAAATGAAAT GATTAATACT 
TGTCAACCAG AAGACCTCAA CCCTGTGTTT GTAGCTGAAG TTGCATTACG ACTAAGTGAA 
ATACTGGAGA TTTTAGGAAA GCCAGAAAAA AAAAGTCCTG GCAGCGCAAG CAGAGTAACT 
ATAGAATTCC AGCAACTTCC TGACAGTTTT CCACTATTTA AGAAAACACC TAAAGAGCAG 
TTACTTTTGG CTCATGAAAC TCTTGAGAAG GCAGTTACTG TGATGGGGCT ATGTCGTTCC 
CAAACTACAT TGCCTAATGG AAAATCAGTA TATGACCATT GTCATATCAA GTTTATCCCT 
CCAAGAGGAG GTGGAACCAC TGGCAAACCA ATCACTGACA ATACTTTCCT GATGGACTTG 
CATCTAGAAC TCCTTGTGAG TCAGCACCGG ATAGCTGCTA CACTCATTGA TCAACTCAAA 
GCTTGGAATT TTTCTCCAAA ACCTGCCCAT CCCAAAGAAA CTGAAACTAC AACTACTAGT 
CATTTCACAG AAGCATACGT ACTGGGCAAA ATCAATAAGA ATGAGTTATC CAAAGCAGTC 
TTCTTAATGA TGAAAGCTCA GATACTATTT GAGAAAGAAG ATTCTCAGGT CATCAAATCA 
CTGGAGGAAT CGTTGTTCTT AATTCGGAAG ATTGAATCAG AGCAAAAAAA CCAGTATTCA 
CCTCAGCAGA GTACAGAAGA AGAAAAACCC CAGAGCAAAG TTCCTGCTCC TCCCATACTA 
CTTTCAAGAA CTCATTGTTC AGTGACATTT AAACCTGATT CATTTTTTTG TGGAGAAAAG 
GTCGCTTGGT ATGTCATTTT GGGACGTCCA GCAGGAACAT GCATGAGAAA AGTAAGATTG 
AACGACCATA GTCTTCTAAA CTGTGGAGAA CCAGTACCTG CTACTAGTAA AACCGTTTTA 
GAAGTTGTTG GATTGGAGCC TAATGAAAAA TACGTGTTTG CACTTGCTGG TTATTGCTCT 
GCTGGAACAC TTATTGGGCA AGGAATTGGA CTGACATCTA AGCCTATTCT GATTTACATG 
CCACTTTCTA CTGTTACTAT TCGCTTACAT CTGACTGAGA TTGCCTTTCA ACTCGGTAAA 
TATTCAGTGG CCAAGAAAGC CTTTGCACCT GTTTGGGACT ATTTCGTCGA TCCTCCTCTT 
CCAGAAAATC CACACATTTT TTCTTTAAGT AGCTTGTTAA CTGTCACACC ATTCAGGTTA 
CATCTGGAGA CCGTGTCAGA GTCTTCATCA ATCCTCTTAT ACTATTTCAT TAGATGCATT 
TTCATAATAA GTAATATTAA TATTATAGAA AAAAAGCTCT TCTGTGATAC TATTAGAGGA 
GATGAGGTTT TCACTTGTCA ACAAGTTTTC AGACTACTTG AATGTCAGAG AATCCTTGTG 
GTCATTGAAC TTAGTACCCA TGTAAATAAT TTTAGTTATT CCCTACAAGC TGTTGTTCAC 
TGCTATGGTC TCCTGGCCCC TCTAATCTTT CACAATATTG TCCTGGTTCC AATAATCCAG 
GTCCTAATTA AGTGTTTGGT AATTTTGCAA GAAATCCCAG GTGTTACCTT TCCAAAGAGA 
CAGATGGGGA ATTTTGAGTC TACCCAGCAT ATGATTGCGT GCACTGTTTA TTACACGGCA 
AAGGTGTTAC GTTCATGGAA GGAATTTGAC CTTGCAGTGA TTATTATAAA TTTGGGGAAA 
AAGTTACTGG ACACCTCACA AGGGTTATTT TCAAATGTCA CTAAAGCTGC CTTTGGTGAG 
GATGATCAAG ATGACTTATC AGAGGAGGCC TACTCAACCA AGAGAATCAA GAGGGTCAAG 
GGACAGATTG CCATACTGGA CAAAATCAGC GAGCAGCTCA ACGTCATGGA AGCTCAACTA 
CTCAAACTGA CGAAACAAAG TCCCATGTAT GAGCTCACAG GAACAGAAGA TCCCTTTTTC 
CTCTACCCAA TAATTCTAAC TTGGACTCTC AAAACTGCGA TGAGGGAAGT AATGAAATTC 
AAGAGTAAAC CTCGTTTTCT GGAATTCTTT GTTCAAGTTA TGACAAAATG TCTTATTGAA 
GAGAAATTTC ACTATGTGGT GGAATTGGCA TTTTCAGTTC AACTCTTCCT TAAAAGGAGA 
AATGCCAGTT TACTTGGAGT AAGGAGATCT CCAGTTCTTC ACACTCTTCC ATCTAATGAA 
GAACCAAAGA AATACAAGGC AGCTGCCATG GAGATTCAAA AAACTACAGA TGTATCAATT 
GGGAAGTCAA AATCAAAGAA AAAAGGTATT CTGCAAGATT ATTTTGTCAA GCATCTCTCA 
CTTTCTGAAT TGCCAGATTT GGAAAAATTT AAAAGAGCTG ATGTGAGAAA AGTAGCTATA 
AAAATCCTTT CAGAACACTT GGCCCCAAAA CTAAATCGCT ATATAAAAAG GAAAAGAATG 
CATCGAATAT TTATTGAAGA GTTACCCTGG AAGTCTCAGA TGAATGTACA TTTGGCTGAA 
GCTCACTTCA ACTTATTCAA AATCAGATTC ATGGAGCGTA AGAAGGTTAA AGCTTGCACT 
TCACAAAATA CAATGAGCTA CCGAATTTTA GACCCGGATA TCTTCTCAAT GTACAATTCT 
GGGACGTTGT TAGTGGATGA AAACTTAAAT TCAGATAATT ACCAAGCATT GCTTGATTTC 
ATGCTTTCAA GAAAAATGAG AAAGATCAAT CTCTCAGATG CCAATGAAAT TTCTTTTGGT 
GGTGTCAGAT TGAGTGAAGA TATCCCTAAA GGGGTAAACA CAAATGACCT GGAATCCGCT 
AGAGAGAAAA CAGCAAATCT TGGAATCATA GACCATTTTA TCAGGACCTT TGTTCACTGC 
AAAAGAGCAT TGGTTCTTGC TCATCGTGGT GGTTTCTGGA CCTTACTTCA GAATGTGACC 
CGGACTCTTT GGAATTACAC CATAGAGATG CAGTTACTCT ATAAACAGGC GCAGTTTCTT 
TCCAAAACAT TCCCTTTCAG CAAGGATATT ATGATATGCA CCTTCGTGAT GCCATTTACT 
TTGGGAGCAG ATGCCCTTAT TGACATGCTG TTAGACCTGC AGAATACCAA ATCTCTTAAG 
ATTGTGGATG ACTGTAAAGA TTTTGGTGTT GCAAGTGCTT ATGGGAACAT CACAGAGGAT 
AATGGTGGCT TCAATCTGAC ATTTGAAGAA CCTTTTGATG ATATGAATTT GGTTGACTTG 
AAATTCATTT CTGACTTCAT CTTTAAGACT TTGGAAATCT TGTACAGAGT GGGAAAATGG 
GAAACACTAG TCTACATTGC CCTTCAGTTC AATGAAGTGA CACATGAGAG ATACACTGAG 
CAAGTGATGC CATTGTTAAT CTATTCTCAA AGACAGCTTT TGCAGAGACT CCAGAGAAAT 
AGTGGGGAGC AGACAGATAA TATAGAATGG CAGTTTGATG ACACGGTAAA CCTCCGAGAT 
GTCATTGGGA AACTGCCCAA TTCTTCTTTT GTGCAGACTA TAAAGGGCAA GTCTAAAAGA 
CTTTTTAATT ATGAGCAAGG TGTAGATCTT TCGGAGACAT ACATTGCAAA TAGAATAGTG 
TCTGTGCCCC TGAATGTGAA AGAAACTTTG AAGACCTTTA GAGAAACCTT GACGAAATCT 
AAGTACCACA ACAGATCACT GAGATACAGC AGAAAATTGC TTTCTTTGTT ACTGGCCCAC 
ACACAAGAAG CCAGTGGAGG AATAAACAAT CCCAAATTTA TGTATGGAAA AGTGGAATTT 
AGCCTTGGAA CTGAAGAAAT ACATTCGCCT ACACCCCCTG ATCTTTTTAA TGAAAAGTTC 
ACTGCTTCTG TTACAGTACA GAGCACAAAA ATTTCTCCTT TCCAATTTCA ACTTGTTATC 
ACTTCTTATG AGAAAACGAT CGAGATTCTT GAAATCAGCA ATCAAACAGA TCTCAAGATA 
CAGGCACTAC ATGAGCTTGG AAATCTTCAC ATCTATGCAG AAAACAGAAG GGCAGCTTTT 
AAATGCTGGA GTCAAGCTCT TGATGCCATA CTCAAAAAAA AAGATGTTCT ACATACGTGG 
AAAGAAATTG TCAGCCCCAT CCAAAAACCT ATGAATAGCA TCGTGGGTTC AAACTACATC 
GATTATAGTG AAGATTTTCT GTTGAAAGCT GGCATTTGGG GATGCTTGCA GGGAGGAGTC 
ATAGCAGCAA AGATGGCCCA ATACATTTTA ACATCAAATA TGAATGAGAG GATTGAATGT 
TGCATTTTCT CTTCATTTTT TTTTAAGGGT CTACTTAGAA CCTCTCTTGT CCATCCCAAA 
TATGACCATG AGTATGCCCG TTATGAAATC ACCCAACTTA CTCCAGGGCT GGATCTCTTC 
TGTGACCGAT ACAGAGCAGA TGTGTCCATT GTCCTCAGCA GCCTGAGTTA CCTCTGTCAT 
GAACTCCATT CTGTCCACCA AGACCTTATT CTTCTGCCAC TTATTTCATT AAACCATTAC 
TTGGTCTGTG AAGTTTGCCA AGATCTATTT AAAACCATAG ATACAAGGAT CCTTAAGACG 
GAAATCCTTA TTGATTTAGG ATTCTTTTCT GAAGCTTTTA GTGAAATGGC TGGTCTTTAT 
ACTGGAAAAA ACATCCCCAC TTCCACGCCC TTAGGCTATA AACTTCCAAA AACTAAGATC 
ATGCAGCCCT TTCAAGCAGG ACACTCTTAT TTGAGCAAAG AAAATATACA GGCCCTGGAA 
GATTTAATGA ATCAAGGCAT CCCCCTATAC ATTATTTCGA TTTGCCAACC ATCTTTCTCC 
TATAAGTTTA CTTTGGTCAA AATGCGCTTT TTAATCAGTT TGGCTGCAAC CATAAATTGC 
TTCCCAGTTA GGGATACAAC AGCATTCTTC CAGGACTTTA CCCAGAAGAG CAAAGCCAGT 
GCTTCAGTCT TTAAAGGTAC AAACCCTGAA GATTCAATAA TCCCCTCTCA AGAGACAGTT 
CAAAATGACT TTACCGCAGC TGAAGGAACA CAGAGAGCTC TGGACAAGAG CATTGACTTC 
TCAAAGCAAA GAGGCTTCGA TGATATGAGG AAGATGCTGA TGGTGGACCC AGAATATTTA 
AAGGATGAAA CCCCAGCAAA TGTTAACCTG ACCAAGTCAA AAGAAGAGTT GTCTCCAGGT 
AAACTAAAGG GGATTTTATT GGCAGAAGCT GAGGACAAGC TTCTCATGCT AATAGAAAAT 
GCAGAGGAAT TGAGTCACAA GAAGATAACT CAGTTTAGCC CAATAGAATT GGAGATTATG 
ATAGAAGTCA AGTTACAGCT AGCCGCTATT GCGATGCAGA GACAACAAGC CCCACTGAGC 
ACTTCAATAG CTTTCACTGC AATTAAAATC CTTCAGGATG CAGACATTTT CAAGAAGACT 
AAAGAAGAAC TGTGTGCGAA TGATGATGAA CTGGATGCCA TGGAGTCTGT GGAGATGTTG 
GCCCGAGAAC GTCTAAACAT CCATCTATGG CTAAAGTGTC GTCTGGCCTT GCTGGTGGCC 
ACTGTGACCC ACATCCGTGG CATGGGGGTC GTGAAAGAAA ATGAGATGAT TGAGTGCAGA 
CTTTTGATAA ATGAAGTATT TGTGGAGGCT CAAAACTGGA ATGATACTGA GATGCAAGCA 
GAAGTCATGG TGCAGGCTGT CAAGTTTGAC CTGCAAGAAA GACATTCTGC AGCCAACATC 
TTAGAACATT TGAAGGATAT AAGAGGCTTA CTTGAAGGAA AATCCTTTGT CTCTCCTCGA 
GGCTCTTTAA TACTTATGAA AAGCCTGCTG TTAATGGATG ATTTAATGAA AATTGAGATG 
CCCCAGGAAT ATCTCTTCCT TAAAAATGAA AATCTCAATT TACTAAATCG AGCTCATGAG 
CTTATGATTG CGCAGGCTCT ATCTTTGGGT GAAAACATTT TATTGCCTTG TTCAGACACT 
GAATATGCAA ATATCTTCTA CCCTTTGAAA AACGTTTATT TTCCATACAT CAACTTACTT 
GCAAAAACAA AAATGAGGAT TGGGCATATG TTAGCCAAGA GCACTGCTGC CTGTGAGAGA 
AAAGAACCTC AGAAGTGGGA ACCCATTCGT CATCTCTTTG AGACAGCATT GCAACTTTGT 
AGGGCAGCTG CAGTGAAGGA ACGGGAGTTA GAAGCCGAAA TCCTTTTTGA GAAAGGCAAA 
GTAGAACGTC AAATGCTTGT TGTACAAGAC AACAAGCCTT TAATTGGTGA TCTTTTTGAT 
GCTATAAAAA TAAGTCTACA AAATGATGAA AATTCAGGGT TGATAAGGAA ATCCTATCTG 
GAAATCGCCC TGTTATATTT ACTTGTGTGG AATTATAGGA AAAATGCTTC AAAAAATAAG 
ATCTTTCTCC AAGTCCAGAA TCCATCAAAG GAATCACTAG AAATTAAAAC TGAAATTTAC 
CTGGCAATGT CCTGGATAGC AATCCGAGCT GCTGCACAGG TCAGTGAAGC TACAATGGCA 
GCTAAGAAGC TAACTGGAGA AAAGAGTGTG AAGACCAAGA AAATGGATCA AAATGTCTAT 
CAGAATATCC CTGAATTTGC TCATGCTGAC CTCACTTGTA CCTATGTAGA TTACTTGACT 
GCTAACTATA AAACTTGTGT CAAAACTTCC TATACCACTC CGAGCGAATC TGGAAGTTAT 
TTAAGTATGG AACAAGATCT GTACACCAAC CCCAGTGAAG AAAATGAGGA GCCCAGCCAG 
CTGGAGATCA CCTGGGTCCA TCTGGCCCGT TACTACACTC ACCTGTTAAG AGTTAACAAT 
ATGAATACCT TGCTTGCTCC TCCAAAGCCA GTTTTCTTAA CAGATGAAAT TTTCTACACT 
TCTGTTTTCA ATGAAGATCT GATTATACGT CAGAAAGAGA TGAAGAATTT CCTCAAAAAA 
TTCTTAAGTG TTTTTACTTC AGCCTGTCTA GAGTGTTTCC CCAAAGACCT CCTTCTTGGA 
ATAGAAAGAC TAAATATTCC AGATAATGCC GAACTTAGGC AATCACTTAA GACATTTCAA 
GATGTGAGTG CTATAATAGA AAAAATAGTC ACCACTAAGA TCAACCCCAT TATCCTTCCT 
CCATCTCCCC CGCATGAACA TATGGATTTT GCTATACATG CAGAAAGCCC AGATCTTTGC 
TTCCAGTGGT ATATACCCCC TCTGGAAAAA CCTCCAAGTG ATAAAGAACC CATGGTCATG 
TTGCTGTATG CCTATAACAC CAAACCTGTC AAGATTAATG ATATCTTTGA ATTCAATATC 
TCCTCGATAT TTCCTGGGTT TTTATGGCTT CCTCTGAACA AAGTTATAGC CTTACATGAA 
GCTCTATCTG GCCTTAGGCA AGAAACTGAA ATGTTGTTAT ACACAAAAAA ATTGCCTACA 
GAAGAAAAGA AGTCCCTACT ACCCTTCAGT GCCTTAAAAC CTGCTAGAGA AGTCTCTGAA 
GAGATCGTCC TCAGTAAGCA AATGAAGACT GTGTTTTATC AGAGTTGCAC TGAAATAGCA 
AGACTGCTTT CGAGAAATGA AGACATACCC CTAATAAGTG AGGTTCCATT TGAAGAATCT 
TTGCCAAGTA TACAGAAGTT GGAGATGCTT TTTGACCCCT CCAGTGGCTC TGTCTTAAAG 
GCAGAAGCCA TTTTCAACTG GATCGTGTCT TTTCTTACAT CTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP54 ( XM_016425735.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281221.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA.

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CloneID	OMc45044
Clone ID Related Accession (Same CDS sequence)	XM_016425735.1
Accession Version	XM_016425735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG TTCCTACTCG CAGGCCCGAA AGTACCTTCA CCTTGTTCAA TATCTGGTTC 
AAGTATCAGA ACCGCCTGCC AGTCCAGTAT TACCATGAGC ATCTTTTGAA GACTGGAGAC 
ACCCTTATTC AAGCAAAAGA ATACAAGTTG GCCTTAATAC AATGCTATGG GAAGTATCTT 
AGTCAGTTCA ATCCCAGTCA CGATGAGAGT GACATGAACG TGACAACTTT CAAAAAAGTC 
TTTTTCTCCA AAGGTGTCAA TGATGAAAGC GCCAAACTTA CATTTCACGC TTTGCAAGGG 
AAATGTATTA GCCAGTACCA AATTATCTGT GCCACCGATC CCTGTTTCAA GAGGGAGCTG 
TCTGTGGAGC AGTGCTTCAA GATTCTGTCG GTTTTTCGGC TCATGATGCA GATAACCCTG 
CCCTACGAAC ACCTTTGCTG GATCGTGTAC AATGGTACAA TTCACATTTA TACAATTTGC 
AGGAAGCTGA TGACCATTGG ACAGTCTGCT AAGGCCTTGG AATTCTTGCT CTGGGCCAGT 
ATTTGTATGG AATCCTCTTT ACCCCTCTTA GCAGTAAGCT ATCTCAGTTG GAGAGCTACC 
CTGTATACTG CTGTTTGCCA GTGCTACTAT GACTGCCATG CTGGAATGCA GGGAGAGATA 
TTTGCTCGGC GAGGTTTGGG AAAAATTGAT GATTTATTAC GTCTAGAAGT AATGAGTATC 
ACACCACCAC AGGAGGAAGA AAGAAAAAAA TTCCGAGAGG CCACCATCAA GATGGCGGTG 
ATGATCTTTA AGAGAGGAGT CTTTGAACCA CGAAGAAAGA GTAAGTCTTA TGTAAGAACA 
AAACTGAGAC TGAACCTACG GGAAGCACAG AATTTGCAAT GGCCACGCAC AGCTACAGAG 
AAGTTGTTGA GTGAGATGTT TGATTGTGGA TCAGCCCAGT TTTTGGCTGT CTTGGAAGCC 
CTTTCTGAAC CAAATAGAAG AGTGTTACAA ACTGGTCACT TGTCAGAGGA AATGGAAGTT 
CGTGAGGTCA TCGCTGAGCT CTTCATGGCA GCAAGCGAGC TTTTATCAGA TGCATGTAGT 
AGCCATGGGA AATCCAGTTT TTCCAAAAAC ATCTCTCTAC AGCTTTCTCA AATGCAACTC 
GCCATTGAAA GCAAACAAAG TGTTTCAATA GCTGCTGCAG TGAAGTTGAT AAAATTATCT 
TTCACCTTTG AGGAATGGCA TTTGTTTAAT TCTGCTGCTT CTCAGTTTAT CGGTTTTCTT 
CAGCTTCAAA GTGGCCCACA CTTGCAGAAA GCCAGGAAGG ATTTAATGCT TCTGGCTGCG 
ATGGAACCGC TAATTAATGT GAAGCGGAAT AAAGGCTTAA GCTATGCTGG TGAAGGAGGC 
AGCACTACCA CTGAAGGAGC TCGAAGCTCA GGGAGAAAAA TTGTGTTTCA AGAAAATTTC 
TCATTATCTG GAAGCTATTC TGATGATATT TTCTATTTGG CATCGATGCT GTTTTCCTTC 
ATTTGTTCAC CTACTGAGAA AATTCGTCCT GATGAAGAGA TCGTTGTTGA CCTGATAATG 
TTCCTGTGGC AGAAATGCAA AATGGGAATT CAGAAGATCT ATTCAGCAAG AATGGATTAT 
TTCAAGTTTG TCCAGAAAGT CAAAACTAAC AAATGGATTC AGCTGCTTTG GCAGATAAAT 
GAAATGATTA ATACTTGTCA ACCAGAAGAC CTCAACCCTG TGTTTGTAGC TGAAGTTGCA 
TTACGACTAA GTGAAATACT GGAGATTTTA GGAAAGCCAG AAAAAAAAAG TCCTGGCAGC 
GCAAGCAGAG TAACTATAGA ATTCCAGCAA CTTCCTGACA GTTTTCCACT ATTTAAGAAA 
ACACCTAAAG AGCAGTTACT TTTGGCTCAT GAAACTCTTG AGAAGGCAGT TACTGTGATG 
GGGCTATGTC GTTCCCAAAC TACATTGCCT AATGGAAAAT CAGTATATGA CCATTGTCAT 
ATCAAGTTTA TCCCTCCAAG AGGAGGTGGA ACCACTGGCA AACCAATCAC TGACAATACT 
TTCCTGATGG ACTTGCATCT AGAACTCCTT GTGAGTCAGC ACCGGATAGC TGCTACACTC 
ATTGATCAAC TCAAAGCTTG GAATTTTTCT CCAAAACCTG CCCATCCCAA AGAAACTGAA 
ACTACAACTA CTAGTCATTT CACAGAAGCA TACGTACTGG GCAAAATCAA TAAGAATGAG 
TTATCCAAAG CAGTCTTCTT AATGATGAAA GCTCAGATAC TATTTGAGAA AGAAGATTCT 
CAGGTCATCA AATCACTGGA GGAATCGTTG TTCTTAATTC GGAAGATTGA ATCAGAGCAA 
AAAAACCAGT ATTCACCTCA GCAGAGTACA GAAGAAGAAA AACCCCAGAG CAAAGTTCCT 
GCTCCTCCCA TACTACTTTC AAGAACTCAT TGTTCAGTGA CATTTAAACC TGATTCATTT 
TTTTGTGGAG AAAAGGTCGC TTGGTATGTC ATTTTGGGAC GTCCAGCAGG AACATGCATG 
AGAAAAGTAA GATTGAACGA CCATAGTCTT CTAAACTGTG GAGAACCAGT ACCTGCTACT 
AGTAAAACCG TTTTAGAAGT TGTTGGATTG GAGCCTAATG AAAAATACGT GTTTGCACTT 
GCTGGTTATT GCTCTGCTGG AACACTTATT GGGCAAGGAA TTGGACTGAC ATCTAAGCCT 
ATTCTGATTT ACATGCCACT TTCTACTGTT ACTATTCGCT TACATCTGAC TGAGATTGCC 
TTTCAACTCG GTAAATATTC AGTGGCCAAG AAAGCCTTTG CACCTGTTTG GGACTATTTC 
GTCGATCCTC CTCTTCCAGA AAATCCACAC ATTTTTTCTT TAAGTAGCTT GTTAACTGTC 
ACACCATTCA GGTTACATCT GGAGACCGTG TCAGAGTCTT CATCAATCCT CTTATACTAT 
TTCATTAGAT GCATTTTCAT AATAAGTAAT ATTAATATTA TAGAAAAAAA GCTCTTCTGT 
GATACTATTA GAGGAGATGA GGTTTTCACT TGTCAACAAG TTTTCAGACT ACTTGAATGT 
CAGAGAATCC TTGTGGTCAT TGAACTTAGT ACCCATGTAA ATAATTTTAG TTATTCCCTA 
CAAGCTGTTG TTCACTGCTA TGGTCTCCTG GCCCCTCTAA TCTTTCACAA TATTGTCCTG 
GTTCCAATAA TCCAGGTCCT AATTAAGTGT TTGGTAATTT TGCAAGAAAT CCCAGGTGTT 
ACCTTTCCAA AGAGACAGAT GGGGAATTTT GAGTCTACCC AGCATATGAT TGCGTGCACT 
GTTTATTACA CGGCAAAGGT GTTACGTTCA TGGAAGGAAT TTGACCTTGC AGTGATTATT 
ATAAATTTGG GGAAAAAGTT ACTGGACACC TCACAAGGGT TATTTTCAAA TGTCACTAAA 
GCTGCCTTTG GTGAGGATGA TCAAGATGAC TTATCAGAGG AGGCCTACTC AACCAAGAGA 
ATCAAGAGGG TCAAGGGACA GATTGCCATA CTGGACAAAA TCAGCGAGCA GCTCAACGTC 
ATGGAAGCTC AACTACTCAA ACTGACGAAA CAAAGTCCCA TGTATGAGCT CACAGGAACA 
GAAGATCCCT TTTTCCTCTA CCCAATAATT CTAACTTGGA CTCTCAAAAC TGCGATGAGG 
GAAGTAATGA AATTCAAGAG TAAACCTCGT TTTCTGGAAT TCTTTGTTCA AGTTATGACA 
AAATGTCTTA TTGAAGAGAA ATTTCACTAT GTGGTGGAAT TGGCATTTTC AGTTCAACTC 
TTCCTTAAAA GGAGAAATGC CAGTTTACTT GGAGTAAGGA GATCTCCAGT TCTTCACACT 
CTTCCATCTA ATGAAGAACC AAAGAAATAC AAGGCAGCTG CCATGGAGAT TCAAAAAACT 
ACAGATGTAT CAATTGGGAA GTCAAAATCA AAGAAAAAAG GTATTCTGCA AGATTATTTT 
GTCAAGCATC TCTCACTTTC TGAATTGCCA GATTTGGAAA AATTTAAAAG AGCTGATGTG 
AGAAAAGTAG CTATAAAAAT CCTTTCAGAA CACTTGGCCC CAAAACTAAA TCGCTATATA 
AAAAGGAAAA GAATGCATCG AATATTTATT GAAGAGTTAC CCTGGAAGTC TCAGATGAAT 
GTACATTTGG CTGAAGCTCA CTTCAACTTA TTCAAAATCA GATTCATGGA GCGTAAGAAG 
GTTAAAGCTT GCACTTCACA AAATACAATG AGCTACCGAA TTTTAGACCC GGATATCTTC 
TCAATGTACA ATTCTGGGAC GTTGTTAGTG GATGAAAACT TAAATTCAGA TAATTACCAA 
GCATTGCTTG ATTTCATGCT TTCAAGAAAA ATGAGAAAGA TCAATCTCTC AGATGCCAAT 
GAAATTTCTT TTGGTGGTGT CAGATTGAGT GAAGATATCC CTAAAGGGGT AAACACAAAT 
GACCTGGAAT CCGCTAGAGA GAAAACAGCA AATCTTGGAA TCATAGACCA TTTTATCAGG 
ACCTTTGTTC ACTGCAAAAG AGCATTGGTT CTTGCTCATC GTGGTGGTTT CTGGACCTTA 
CTTCAGAATG TGACCCGGAC TCTTTGGAAT TACACCATAG AGATGCAGTT ACTCTATAAA 
CAGGCGCAGT TTCTTTCCAA AACATTCCCT TTCAGCAAGG ATATTATGAT ATGCACCTTC 
GTGATGCCAT TTACTTTGGG AGCAGATGCC CTTATTGACA TGCTGTTAGA CCTGCAGAAT 
ACCAAATCTC TTAAGATTGT GGATGACTGT AAAGATTTTG GTGTTGCAAG TGCTTATGGG 
AACATCACAG AGGATAATGG TGGCTTCAAT CTGACATTTG AAGAACCTTT TGATGATATG 
AATTTGGTTG ACTTGAAATT CATTTCTGAC TTCATCTTTA AGACTTTGGA AATCTTGTAC 
AGAGTGGGAA AATGGGAAAC ACTAGTCTAC ATTGCCCTTC AGTTCAATGA AGTGACACAT 
GAGAGATACA CTGAGCAAGT GATGCCATTG TTAATCTATT CTCAAAGACA GCTTTTGCAG 
AGACTCCAGA GAAATAGTGG GGAGCAGACA GATAATATAG AATGGCAGTT TGATGACACG 
GTAAACCTCC GAGATGTCAT TGGGAAACTG CCCAATTCTT CTTTTGTGCA GACTATAAAG 
GGCAAGTCTA AAAGACTTTT TAATTATGAG CAAGGTGTAG ATCTTTCGGA GACATACATT 
GCAAATAGAA TAGTGTCTGT GCCCCTGAAT GTGAAAGAAA CTTTGAAGAC CTTTAGAGAA 
ACCTTGACGA AATCTAAGTA CCACAACAGA TCACTGAGAT ACAGCAGAAA ATTGCTTTCT 
TTGTTACTGG CCCACACACA AGAAGCCAGT GGAGGAATAA ACAATCCCAA ATTTATGTAT 
GGAAAAGTGG AATTTAGCCT TGGAACTGAA GAAATACATT CGCCTACACC CCCTGATCTT 
TTTAATGAAA AGTTCACTGC TTCTGTTACA GTACAGAGCA CAAAAATTTC TCCTTTCCAA 
TTTCAACTTG TTATCACTTC TTATGAGAAA ACGATCGAGA TTCTTGAAAT CAGCAATCAA 
ACAGATCTCA AGATACAGGC ACTACATGAG CTTGGAAATC TTCACATCTA TGCAGAAAAC 
AGAAGGGCAG CTTTTAAATG CTGGAGTCAA GCTCTTGATG CCATACTCAA AAAAAAAGAT 
GTTCTACATA CGTGGAAAGA AATTGTCAGC CCCATCCAAA AACCTATGAA TAGCATCGTG 
GGTTCAAACT ACATCGATTA TAGTGAAGAT TTTCTGTTGA AAGCTGGCAT TTGGGGATGC 
TTGCAGGGAG GAGTCATAGC AGCAAAGATG GCCCAATACA TTTTAACATC AAATATGAAT 
GAGAGGATTG AATGTTGCAT TTTCTCTTCA TTTTTTTTTA AGGGTCTACT TAGAACCTCT 
CTTGTCCATC CCAAATATGA CCATGAGTAT GCCCGTTATG AAATCACCCA ACTTACTCCA 
GGGCTGGATC TCTTCTGTGA CCGATACAGA GCAGATGTGT CCATTGTCCT CAGCAGCCTG 
AGTTACCTCT GTCATGAACT CCATTCTGTC CACCAAGACC TTATTCTTCT GCCACTTATT 
TCATTAAACC ATTACTTGGT CTGTGAAGTT TGCCAAGATC TATTTAAAAC CATAGATACA 
AGGATCCTTA AGACGGAAAT CCTTATTGAT TTAGGATTCT TTTCTGAAGC TTTTAGTGAA 
ATGGCTGGTC TTTATACTGG AAAAAACATC CCCACTTCCA CGCCCTTAGG CTATAAACTT 
CCAAAAACTA AGATCATGCA GCCCTTTCAA GCAGGACACT CTTATTTGAG CAAAGAAAAT 
ATACAGGCCC TGGAAGATTT AATGAATCAA GGCATCCCCC TATACATTAT TTCGATTTGC 
CAACCATCTT TCTCCTATAA GTTTACTTTG GTCAAAATGC GCTTTTTAAT CAGTTTGGCT 
GCAACCATAA ATTGCTTCCC AGTTAGGGAT ACAACAGCAT TCTTCCAGGA CTTTACCCAG 
AAGAGCAAAG CCAGTGCTTC AGTCTTTAAA GGTACAAACC CTGAAGATTC AATAATCCCC 
TCTCAAGAGA CAGTTCAAAA TGACTTTACC GCAGCTGAAG GAACACAGAG AGCTCTGGAC 
AAGAGCATTG ACTTCTCAAA GCAAAGAGGC TTCGATGATA TGAGGAAGAT GCTGATGGTG 
GACCCAGAAT ATTTAAAGGA TGAAACCCCA GCAAATGTTA ACCTGACCAA GTCAAAAGAA 
GAGTTGTCTC CAGGTAAACT AAAGGGGATT TTATTGGCAG AAGCTGAGGA CAAGCTTCTC 
ATGCTAATAG AAAATGCAGA GGAATTGAGT CACAAGAAGA TAACTCAGTT TAGCCCAATA 
GAATTGGAGA TTATGATAGA AGTCAAGTTA CAGCTAGCCG CTATTGCGAT GCAGAGACAA 
CAAGCCCCAC TGAGCACTTC AATAGCTTTC ACTGCAATTA AAATCCTTCA GGATGCAGAC 
ATTTTCAAGA AGACTAAAGA AGAACTGTGT GCGAATGATG ATGAACTGGA TGCCATGGAG 
TCTGTGGAGA TGTTGGCCCG AGAACGTCTA AACATCCATC TATGGCTAAA GTGTCGTCTG 
GCCTTGCTGG TGGCCACTGT GACCCACATC CGTGGCATGG GGGTCGTGAA AGAAAATGAG 
ATGATTGAGT GCAGACTTTT GATAAATGAA GTATTTGTGG AGGCTCAAAA CTGGAATGAT 
ACTGAGATGC AAGCAGAAGT CATGGTGCAG GCTGTCAAGT TTGACCTGCA AGAAAGACAT 
TCTGCAGCCA ACATCTTAGA ACATTTGAAG GATATAAGAG GCTTACTTGA AGGAAAATCC 
TTTGTCTCTC CTCGAGGCTC TTTAATACTT ATGAAAAGCC TGCTGTTAAT GGATGATTTA 
ATGAAAATTG AGATGCCCCA GGAATATCTC TTCCTTAAAA ATGAAAATCT CAATTTACTA 
AATCGAGCTC ATGAGCTTAT GATTGCGCAG GCTCTATCTT TGGGTGAAAA CATTTTATTG 
CCTTGTTCAG ACACTGAATA TGCAAATATC TTCTACCCTT TGAAAAACGT TTATTTTCCA 
TACATCAACT TACTTGCAAA AACAAAAATG AGGATTGGGC ATATGTTAGC CAAGAGCACT 
GCTGCCTGTG AGAGAAAAGA ACCTCAGAAG TGGGAACCCA TTCGTCATCT CTTTGAGACA 
GCATTGCAAC TTTGTAGGGC AGCTGCAGTG AAGGAACGGG AGTTAGAAGC CGAAATCCTT 
TTTGAGAAAG GCAAAGTAGA ACGTCAAATG CTTGTTGTAC AAGACAACAA GCCTTTAATT 
GGTGATCTTT TTGATGCTAT AAAAATAAGT CTACAAAATG ATGAAAATTC AGGGTTGATA 
AGGAAATCCT ATCTGGAAAT CGCCCTGTTA TATTTACTTG TGTGGAATTA TAGGAAAAAT 
GCTTCAAAAA ATAAGATCTT TCTCCAAGTC CAGAATCCAT CAAAGGAATC ACTAGAAATT 
AAAACTGAAA TTTACCTGGC AATGTCCTGG ATAGCAATCC GAGCTGCTGC ACAGGTCAGT 
GAAGCTACAA TGGCAGCTAA GAAGCTAACT GGAGAAAAGA GTGTGAAGAC CAAGAAAATG 
GATCAAAATG TCTATCAGAA TATCCCTGAA TTTGCTCATG CTGACCTCAC TTGTACCTAT 
GTAGATTACT TGACTGCTAA CTATAAAACT TGTGTCAAAA CTTCCTATAC CACTCCGAGC 
GAATCTGGAA GTTATTTAAG TATGGAACAA GATCTGTACA CCAACCCCAG TGAAGAAAAT 
GAGGAGCCCA GCCAGCTGGA GATCACCTGG GTCCATCTGG CCCGTTACTA CACTCACCTG 
TTAAGAGTTA ACAATATGAA TACCTTGCTT GCTCCTCCAA AGCCAGTTTT CTTAACAGAT 
GAAATTTTCT ACACTTCTGT TTTCAATGAA GATCTGATTA TACGTCAGAA AGAGATGAAG 
AATTTCCTCA AAAAATTCTT AAGTGTTTTT ACTTCAGCCT GTCTAGAGTG TTTCCCCAAA 
GACCTCCTTC TTGGAATAGA AAGACTAAAT ATTCCAGATA ATGCCGAACT TAGGCAATCA 
CTTAAGACAT TTCAAGATGT GAGTGCTATA ATAGAAAAAA TAGTCACCAC TAAGATCAAC 
CCCATTATCC TTCCTCCATC TCCCCCGCAT GAACATATGG ATTTTGCTAT ACATGCAGAA 
AGCCCAGATC TTTGCTTCCA GTGGTATATA CCCCCTCTGG AAAAACCTCC AAGTGATAAA 
GAACCCATGG TCATGTTGCT GTATGCCTAT AACACCAAAC CTGTCAAGAT TAATGATATC 
TTTGAATTCA ATATCTCCTC GATATTTCCT GGGTTTTTAT GGCTTCCTCT GAACAAAGTT 
ATAGCCTTAC ATGAAGCTCT ATCTGGCCTT AGGCAAGAAA CTGAAATGTT GTTATACACA 
AAAAAATTGC CTACAGAAGA AAAGAAGTCC CTACTACCCT TCAGTGCCTT AAAACCTGCT 
AGAGAAGTCT CTGAAGAGAT CGTCCTCAGT AAGCAAATGA AGACTGTGTT TTATCAGAGT 
TGCACTGAAA TAGCAAGACT GCTTTCGAGA AATGAAGACA TACCCCTAAT AAGTGAGGTT 
CCATTTGAAG AATCTTTGCC AAGTATACAG AAGTTGGAGA TGCTTTTTGA CCCCTCCAGT 
GGCTCTGTCT TAAAGGCAGA AGCCATTTTC AACTGGATCG TGTCTTTTCT TACATCTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281221.1
CDS	88..9390
Translation	MDDVPTRRPESTFTLFNIWFKYQNRLPVQYYHEHLLKTGDTLIQAKEYKLALIQCYGKYLSQFNPSHDESDMNVTTFKKVFFSKGVNDESAKLTFHALQGKCISQYQIICATDPCFKRELSVEQCFKILSVFRLMMQITLPYEHLCWIVYNGTIHIYTICRKLMTIGQSAKALEFLLWASICMESSLPLLAVSYLSWRATLYTAVCQCYYDCHAGMQGEIFARRGLGKIDDLLRLEVMSITPPQEEERKKFREATIKMAVMIFKRGVFEPRRKSKSYVRTKLRLNLREAQNLQWPRTATEKLLSEMFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_016425735.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425735.1

ORF Insert Sequence:

ATGGATGATG TTCCTACTCG CAGGCCCGAA AGTACCTTCA CCTTGTTCAA TATCTGGTTC 
AAGTATCAGA ACCGCCTGCC AGTCCAGTAT TACCATGAGC ATCTTTTGAA GACTGGAGAC 
ACCCTTATTC AAGCAAAAGA ATACAAGTTG GCCTTAATAC AATGCTATGG GAAGTATCTT 
AGTCAGTTCA ATCCCAGTCA CGATGAGAGT GACATGAACG TGACAACTTT CAAAAAAGTC 
TTTTTCTCCA AAGGTGTCAA TGATGAAAGC GCCAAACTTA CATTTCACGC TTTGCAAGGG 
AAATGTATTA GCCAGTACCA AATTATCTGT GCCACCGATC CCTGTTTCAA GAGGGAGCTG 
TCTGTGGAGC AGTGCTTCAA GATTCTGTCG GTTTTTCGGC TCATGATGCA GATAACCCTG 
CCCTACGAAC ACCTTTGCTG GATCGTGTAC AATGGTACAA TTCACATTTA TACAATTTGC 
AGGAAGCTGA TGACCATTGG ACAGTCTGCT AAGGCCTTGG AATTCTTGCT CTGGGCCAGT 
ATTTGTATGG AATCCTCTTT ACCCCTCTTA GCAGTAAGCT ATCTCAGTTG GAGAGCTACC 
CTGTATACTG CTGTTTGCCA GTGCTACTAT GACTGCCATG CTGGAATGCA GGGAGAGATA 
TTTGCTCGGC GAGGTTTGGG AAAAATTGAT GATTTATTAC GTCTAGAAGT AATGAGTATC 
ACACCACCAC AGGAGGAAGA AAGAAAAAAA TTCCGAGAGG CCACCATCAA GATGGCGGTG 
ATGATCTTTA AGAGAGGAGT CTTTGAACCA CGAAGAAAGA GTAAGTCTTA TGTAAGAACA 
AAACTGAGAC TGAACCTACG GGAAGCACAG AATTTGCAAT GGCCACGCAC AGCTACAGAG 
AAGTTGTTGA GTGAGATGTT TGATTGTGGA TCAGCCCAGT TTTTGGCTGT CTTGGAAGCC 
CTTTCTGAAC CAAATAGAAG AGTGTTACAA ACTGGTCACT TGTCAGAGGA AATGGAAGTT 
CGTGAGGTCA TCGCTGAGCT CTTCATGGCA GCAAGCGAGC TTTTATCAGA TGCATGTAGT 
AGCCATGGGA AATCCAGTTT TTCCAAAAAC ATCTCTCTAC AGCTTTCTCA AATGCAACTC 
GCCATTGAAA GCAAACAAAG TGTTTCAATA GCTGCTGCAG TGAAGTTGAT AAAATTATCT 
TTCACCTTTG AGGAATGGCA TTTGTTTAAT TCTGCTGCTT CTCAGTTTAT CGGTTTTCTT 
CAGCTTCAAA GTGGCCCACA CTTGCAGAAA GCCAGGAAGG ATTTAATGCT TCTGGCTGCG 
ATGGAACCGC TAATTAATGT GAAGCGGAAT AAAGGCTTAA GCTATGCTGG TGAAGGAGGC 
AGCACTACCA CTGAAGGAGC TCGAAGCTCA GGGAGAAAAA TTGTGTTTCA AGAAAATTTC 
TCATTATCTG GAAGCTATTC TGATGATATT TTCTATTTGG CATCGATGCT GTTTTCCTTC 
ATTTGTTCAC CTACTGAGAA AATTCGTCCT GATGAAGAGA TCGTTGTTGA CCTGATAATG 
TTCCTGTGGC AGAAATGCAA AATGGGAATT CAGAAGATCT ATTCAGCAAG AATGGATTAT 
TTCAAGTTTG TCCAGAAAGT CAAAACTAAC AAATGGATTC AGCTGCTTTG GCAGATAAAT 
GAAATGATTA ATACTTGTCA ACCAGAAGAC CTCAACCCTG TGTTTGTAGC TGAAGTTGCA 
TTACGACTAA GTGAAATACT GGAGATTTTA GGAAAGCCAG AAAAAAAAAG TCCTGGCAGC 
GCAAGCAGAG TAACTATAGA ATTCCAGCAA CTTCCTGACA GTTTTCCACT ATTTAAGAAA 
ACACCTAAAG AGCAGTTACT TTTGGCTCAT GAAACTCTTG AGAAGGCAGT TACTGTGATG 
GGGCTATGTC GTTCCCAAAC TACATTGCCT AATGGAAAAT CAGTATATGA CCATTGTCAT 
ATCAAGTTTA TCCCTCCAAG AGGAGGTGGA ACCACTGGCA AACCAATCAC TGACAATACT 
TTCCTGATGG ACTTGCATCT AGAACTCCTT GTGAGTCAGC ACCGGATAGC TGCTACACTC 
ATTGATCAAC TCAAAGCTTG GAATTTTTCT CCAAAACCTG CCCATCCCAA AGAAACTGAA 
ACTACAACTA CTAGTCATTT CACAGAAGCA TACGTACTGG GCAAAATCAA TAAGAATGAG 
TTATCCAAAG CAGTCTTCTT AATGATGAAA GCTCAGATAC TATTTGAGAA AGAAGATTCT 
CAGGTCATCA AATCACTGGA GGAATCGTTG TTCTTAATTC GGAAGATTGA ATCAGAGCAA 
AAAAACCAGT ATTCACCTCA GCAGAGTACA GAAGAAGAAA AACCCCAGAG CAAAGTTCCT 
GCTCCTCCCA TACTACTTTC AAGAACTCAT TGTTCAGTGA CATTTAAACC TGATTCATTT 
TTTTGTGGAG AAAAGGTCGC TTGGTATGTC ATTTTGGGAC GTCCAGCAGG AACATGCATG 
AGAAAAGTAA GATTGAACGA CCATAGTCTT CTAAACTGTG GAGAACCAGT ACCTGCTACT 
AGTAAAACCG TTTTAGAAGT TGTTGGATTG GAGCCTAATG AAAAATACGT GTTTGCACTT 
GCTGGTTATT GCTCTGCTGG AACACTTATT GGGCAAGGAA TTGGACTGAC ATCTAAGCCT 
ATTCTGATTT ACATGCCACT TTCTACTGTT ACTATTCGCT TACATCTGAC TGAGATTGCC 
TTTCAACTCG GTAAATATTC AGTGGCCAAG AAAGCCTTTG CACCTGTTTG GGACTATTTC 
GTCGATCCTC CTCTTCCAGA AAATCCACAC ATTTTTTCTT TAAGTAGCTT GTTAACTGTC 
ACACCATTCA GGTTACATCT GGAGACCGTG TCAGAGTCTT CATCAATCCT CTTATACTAT 
TTCATTAGAT GCATTTTCAT AATAAGTAAT ATTAATATTA TAGAAAAAAA GCTCTTCTGT 
GATACTATTA GAGGAGATGA GGTTTTCACT TGTCAACAAG TTTTCAGACT ACTTGAATGT 
CAGAGAATCC TTGTGGTCAT TGAACTTAGT ACCCATGTAA ATAATTTTAG TTATTCCCTA 
CAAGCTGTTG TTCACTGCTA TGGTCTCCTG GCCCCTCTAA TCTTTCACAA TATTGTCCTG 
GTTCCAATAA TCCAGGTCCT AATTAAGTGT TTGGTAATTT TGCAAGAAAT CCCAGGTGTT 
ACCTTTCCAA AGAGACAGAT GGGGAATTTT GAGTCTACCC AGCATATGAT TGCGTGCACT 
GTTTATTACA CGGCAAAGGT GTTACGTTCA TGGAAGGAAT TTGACCTTGC AGTGATTATT 
ATAAATTTGG GGAAAAAGTT ACTGGACACC TCACAAGGGT TATTTTCAAA TGTCACTAAA 
GCTGCCTTTG GTGAGGATGA TCAAGATGAC TTATCAGAGG AGGCCTACTC AACCAAGAGA 
ATCAAGAGGG TCAAGGGACA GATTGCCATA CTGGACAAAA TCAGCGAGCA GCTCAACGTC 
ATGGAAGCTC AACTACTCAA ACTGACGAAA CAAAGTCCCA TGTATGAGCT CACAGGAACA 
GAAGATCCCT TTTTCCTCTA CCCAATAATT CTAACTTGGA CTCTCAAAAC TGCGATGAGG 
GAAGTAATGA AATTCAAGAG TAAACCTCGT TTTCTGGAAT TCTTTGTTCA AGTTATGACA 
AAATGTCTTA TTGAAGAGAA ATTTCACTAT GTGGTGGAAT TGGCATTTTC AGTTCAACTC 
TTCCTTAAAA GGAGAAATGC CAGTTTACTT GGAGTAAGGA GATCTCCAGT TCTTCACACT 
CTTCCATCTA ATGAAGAACC AAAGAAATAC AAGGCAGCTG CCATGGAGAT TCAAAAAACT 
ACAGATGTAT CAATTGGGAA GTCAAAATCA AAGAAAAAAG GTATTCTGCA AGATTATTTT 
GTCAAGCATC TCTCACTTTC TGAATTGCCA GATTTGGAAA AATTTAAAAG AGCTGATGTG 
AGAAAAGTAG CTATAAAAAT CCTTTCAGAA CACTTGGCCC CAAAACTAAA TCGCTATATA 
AAAAGGAAAA GAATGCATCG AATATTTATT GAAGAGTTAC CCTGGAAGTC TCAGATGAAT 
GTACATTTGG CTGAAGCTCA CTTCAACTTA TTCAAAATCA GATTCATGGA GCGTAAGAAG 
GTTAAAGCTT GCACTTCACA AAATACAATG AGCTACCGAA TTTTAGACCC GGATATCTTC 
TCAATGTACA ATTCTGGGAC GTTGTTAGTG GATGAAAACT TAAATTCAGA TAATTACCAA 
GCATTGCTTG ATTTCATGCT TTCAAGAAAA ATGAGAAAGA TCAATCTCTC AGATGCCAAT 
GAAATTTCTT TTGGTGGTGT CAGATTGAGT GAAGATATCC CTAAAGGGGT AAACACAAAT 
GACCTGGAAT CCGCTAGAGA GAAAACAGCA AATCTTGGAA TCATAGACCA TTTTATCAGG 
ACCTTTGTTC ACTGCAAAAG AGCATTGGTT CTTGCTCATC GTGGTGGTTT CTGGACCTTA 
CTTCAGAATG TGACCCGGAC TCTTTGGAAT TACACCATAG AGATGCAGTT ACTCTATAAA 
CAGGCGCAGT TTCTTTCCAA AACATTCCCT TTCAGCAAGG ATATTATGAT ATGCACCTTC 
GTGATGCCAT TTACTTTGGG AGCAGATGCC CTTATTGACA TGCTGTTAGA CCTGCAGAAT 
ACCAAATCTC TTAAGATTGT GGATGACTGT AAAGATTTTG GTGTTGCAAG TGCTTATGGG 
AACATCACAG AGGATAATGG TGGCTTCAAT CTGACATTTG AAGAACCTTT TGATGATATG 
AATTTGGTTG ACTTGAAATT CATTTCTGAC TTCATCTTTA AGACTTTGGA AATCTTGTAC 
AGAGTGGGAA AATGGGAAAC ACTAGTCTAC ATTGCCCTTC AGTTCAATGA AGTGACACAT 
GAGAGATACA CTGAGCAAGT GATGCCATTG TTAATCTATT CTCAAAGACA GCTTTTGCAG 
AGACTCCAGA GAAATAGTGG GGAGCAGACA GATAATATAG AATGGCAGTT TGATGACACG 
GTAAACCTCC GAGATGTCAT TGGGAAACTG CCCAATTCTT CTTTTGTGCA GACTATAAAG 
GGCAAGTCTA AAAGACTTTT TAATTATGAG CAAGGTGTAG ATCTTTCGGA GACATACATT 
GCAAATAGAA TAGTGTCTGT GCCCCTGAAT GTGAAAGAAA CTTTGAAGAC CTTTAGAGAA 
ACCTTGACGA AATCTAAGTA CCACAACAGA TCACTGAGAT ACAGCAGAAA ATTGCTTTCT 
TTGTTACTGG CCCACACACA AGAAGCCAGT GGAGGAATAA ACAATCCCAA ATTTATGTAT 
GGAAAAGTGG AATTTAGCCT TGGAACTGAA GAAATACATT CGCCTACACC CCCTGATCTT 
TTTAATGAAA AGTTCACTGC TTCTGTTACA GTACAGAGCA CAAAAATTTC TCCTTTCCAA 
TTTCAACTTG TTATCACTTC TTATGAGAAA ACGATCGAGA TTCTTGAAAT CAGCAATCAA 
ACAGATCTCA AGATACAGGC ACTACATGAG CTTGGAAATC TTCACATCTA TGCAGAAAAC 
AGAAGGGCAG CTTTTAAATG CTGGAGTCAA GCTCTTGATG CCATACTCAA AAAAAAAGAT 
GTTCTACATA CGTGGAAAGA AATTGTCAGC CCCATCCAAA AACCTATGAA TAGCATCGTG 
GGTTCAAACT ACATCGATTA TAGTGAAGAT TTTCTGTTGA AAGCTGGCAT TTGGGGATGC 
TTGCAGGGAG GAGTCATAGC AGCAAAGATG GCCCAATACA TTTTAACATC AAATATGAAT 
GAGAGGATTG AATGTTGCAT TTTCTCTTCA TTTTTTTTTA AGGGTCTACT TAGAACCTCT 
CTTGTCCATC CCAAATATGA CCATGAGTAT GCCCGTTATG AAATCACCCA ACTTACTCCA 
GGGCTGGATC TCTTCTGTGA CCGATACAGA GCAGATGTGT CCATTGTCCT CAGCAGCCTG 
AGTTACCTCT GTCATGAACT CCATTCTGTC CACCAAGACC TTATTCTTCT GCCACTTATT 
TCATTAAACC ATTACTTGGT CTGTGAAGTT TGCCAAGATC TATTTAAAAC CATAGATACA 
AGGATCCTTA AGACGGAAAT CCTTATTGAT TTAGGATTCT TTTCTGAAGC TTTTAGTGAA 
ATGGCTGGTC TTTATACTGG AAAAAACATC CCCACTTCCA CGCCCTTAGG CTATAAACTT 
CCAAAAACTA AGATCATGCA GCCCTTTCAA GCAGGACACT CTTATTTGAG CAAAGAAAAT 
ATACAGGCCC TGGAAGATTT AATGAATCAA GGCATCCCCC TATACATTAT TTCGATTTGC 
CAACCATCTT TCTCCTATAA GTTTACTTTG GTCAAAATGC GCTTTTTAAT CAGTTTGGCT 
GCAACCATAA ATTGCTTCCC AGTTAGGGAT ACAACAGCAT TCTTCCAGGA CTTTACCCAG 
AAGAGCAAAG CCAGTGCTTC AGTCTTTAAA GGTACAAACC CTGAAGATTC AATAATCCCC 
TCTCAAGAGA CAGTTCAAAA TGACTTTACC GCAGCTGAAG GAACACAGAG AGCTCTGGAC 
AAGAGCATTG ACTTCTCAAA GCAAAGAGGC TTCGATGATA TGAGGAAGAT GCTGATGGTG 
GACCCAGAAT ATTTAAAGGA TGAAACCCCA GCAAATGTTA ACCTGACCAA GTCAAAAGAA 
GAGTTGTCTC CAGGTAAACT AAAGGGGATT TTATTGGCAG AAGCTGAGGA CAAGCTTCTC 
ATGCTAATAG AAAATGCAGA GGAATTGAGT CACAAGAAGA TAACTCAGTT TAGCCCAATA 
GAATTGGAGA TTATGATAGA AGTCAAGTTA CAGCTAGCCG CTATTGCGAT GCAGAGACAA 
CAAGCCCCAC TGAGCACTTC AATAGCTTTC ACTGCAATTA AAATCCTTCA GGATGCAGAC 
ATTTTCAAGA AGACTAAAGA AGAACTGTGT GCGAATGATG ATGAACTGGA TGCCATGGAG 
TCTGTGGAGA TGTTGGCCCG AGAACGTCTA AACATCCATC TATGGCTAAA GTGTCGTCTG 
GCCTTGCTGG TGGCCACTGT GACCCACATC CGTGGCATGG GGGTCGTGAA AGAAAATGAG 
ATGATTGAGT GCAGACTTTT GATAAATGAA GTATTTGTGG AGGCTCAAAA CTGGAATGAT 
ACTGAGATGC AAGCAGAAGT CATGGTGCAG GCTGTCAAGT TTGACCTGCA AGAAAGACAT 
TCTGCAGCCA ACATCTTAGA ACATTTGAAG GATATAAGAG GCTTACTTGA AGGAAAATCC 
TTTGTCTCTC CTCGAGGCTC TTTAATACTT ATGAAAAGCC TGCTGTTAAT GGATGATTTA 
ATGAAAATTG AGATGCCCCA GGAATATCTC TTCCTTAAAA ATGAAAATCT CAATTTACTA 
AATCGAGCTC ATGAGCTTAT GATTGCGCAG GCTCTATCTT TGGGTGAAAA CATTTTATTG 
CCTTGTTCAG ACACTGAATA TGCAAATATC TTCTACCCTT TGAAAAACGT TTATTTTCCA 
TACATCAACT TACTTGCAAA AACAAAAATG AGGATTGGGC ATATGTTAGC CAAGAGCACT 
GCTGCCTGTG AGAGAAAAGA ACCTCAGAAG TGGGAACCCA TTCGTCATCT CTTTGAGACA 
GCATTGCAAC TTTGTAGGGC AGCTGCAGTG AAGGAACGGG AGTTAGAAGC CGAAATCCTT 
TTTGAGAAAG GCAAAGTAGA ACGTCAAATG CTTGTTGTAC AAGACAACAA GCCTTTAATT 
GGTGATCTTT TTGATGCTAT AAAAATAAGT CTACAAAATG ATGAAAATTC AGGGTTGATA 
AGGAAATCCT ATCTGGAAAT CGCCCTGTTA TATTTACTTG TGTGGAATTA TAGGAAAAAT 
GCTTCAAAAA ATAAGATCTT TCTCCAAGTC CAGAATCCAT CAAAGGAATC ACTAGAAATT 
AAAACTGAAA TTTACCTGGC AATGTCCTGG ATAGCAATCC GAGCTGCTGC ACAGGTCAGT 
GAAGCTACAA TGGCAGCTAA GAAGCTAACT GGAGAAAAGA GTGTGAAGAC CAAGAAAATG 
GATCAAAATG TCTATCAGAA TATCCCTGAA TTTGCTCATG CTGACCTCAC TTGTACCTAT 
GTAGATTACT TGACTGCTAA CTATAAAACT TGTGTCAAAA CTTCCTATAC CACTCCGAGC 
GAATCTGGAA GTTATTTAAG TATGGAACAA GATCTGTACA CCAACCCCAG TGAAGAAAAT 
GAGGAGCCCA GCCAGCTGGA GATCACCTGG GTCCATCTGG CCCGTTACTA CACTCACCTG 
TTAAGAGTTA ACAATATGAA TACCTTGCTT GCTCCTCCAA AGCCAGTTTT CTTAACAGAT 
GAAATTTTCT ACACTTCTGT TTTCAATGAA GATCTGATTA TACGTCAGAA AGAGATGAAG 
AATTTCCTCA AAAAATTCTT AAGTGTTTTT ACTTCAGCCT GTCTAGAGTG TTTCCCCAAA 
GACCTCCTTC TTGGAATAGA AAGACTAAAT ATTCCAGATA ATGCCGAACT TAGGCAATCA 
CTTAAGACAT TTCAAGATGT GAGTGCTATA ATAGAAAAAA TAGTCACCAC TAAGATCAAC 
CCCATTATCC TTCCTCCATC TCCCCCGCAT GAACATATGG ATTTTGCTAT ACATGCAGAA 
AGCCCAGATC TTTGCTTCCA GTGGTATATA CCCCCTCTGG AAAAACCTCC AAGTGATAAA 
GAACCCATGG TCATGTTGCT GTATGCCTAT AACACCAAAC CTGTCAAGAT TAATGATATC 
TTTGAATTCA ATATCTCCTC GATATTTCCT GGGTTTTTAT GGCTTCCTCT GAACAAAGTT 
ATAGCCTTAC ATGAAGCTCT ATCTGGCCTT AGGCAAGAAA CTGAAATGTT GTTATACACA 
AAAAAATTGC CTACAGAAGA AAAGAAGTCC CTACTACCCT TCAGTGCCTT AAAACCTGCT 
AGAGAAGTCT CTGAAGAGAT CGTCCTCAGT AAGCAAATGA AGACTGTGTT TTATCAGAGT 
TGCACTGAAA TAGCAAGACT GCTTTCGAGA AATGAAGACA TACCCCTAAT AAGTGAGGTT 
CCATTTGAAG AATCTTTGCC AAGTATACAG AAGTTGGAGA TGCTTTTTGA CCCCTCCAGT 
GGCTCTGTCT TAAAGGCAGA AGCCATTTTC AACTGGATCG TGTCTTTTCT TACATCTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP54 ( XM_016425736.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281222.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA.

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CloneID	OMc45045
Clone ID Related Accession (Same CDS sequence)	XM_016425736.1
Accession Version	XM_016425736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGA CAACTTTCAA AAAAGTCTTT TTCTCCAAAG GTGTCAATGA TGAAAGCGCC 
AAACTTACAT TTCACGCTTT GCAAGGGAAA TGTATTAGCC AGTACCAAAT TATCTGTGCC 
ACCGATCCCT GTTTCAAGAG GGAGCTGTCT GTGGAGCAGT GCTTCAAGAT TCTGTCGGTT 
TTTCGGCTCA TGATGCAGAT AACCCTGCCC TACGAACACC TTTGCTGGAT CGTGTACAAT 
GGTACAATTC ACATTTATAC AATTTGCAGG AAGCTGATGA CCATTGGACA GTCTGCTAAG 
GCCTTGGAAT TCTTGCTCTG GGCCAGTATT TGTATGGAAT CCTCTTTACC CCTCTTAGCA 
GTAAGCTATC TCAGTTGGAG AGCTACCCTG TATACTGCTG TTTGCCAGTG CTACTATGAC 
TGCCATGCTG GAATGCAGGG AGAGATATTT GCTCGGCGAG GTTTGGGAAA AATTGATGAT 
TTATTACGTC TAGAAGTAAT GAGTATCACA CCACCACAGG AGGAAGAAAG AAAAAAATTC 
CGAGAGGCCA CCATCAAGAT GGCGGTGATG ATCTTTAAGA GAGGAGTCTT TGAACCACGA 
AGAAAGAGTA AGTCTTATGT AAGAACAAAA CTGAGACTGA ACCTACGGGA AGCACAGAAT 
TTGCAATGGC CACGCACAGC TACAGAGAAG TTGTTGAGTG AGATGTTTGA TTGTGGATCA 
GCCCAGTTTT TGGCTGTCTT GGAAGCCCTT TCTGAACCAA ATAGAAGAGT GTTACAAACT 
GGTCACTTGT CAGAGGAAAT GGAAGTTCGT GAGGTCATCG CTGAGCTCTT CATGGCAGCA 
AGCGAGCTTT TATCAGATGC ATGTAGTAGC CATGGGAAAT CCAGTTTTTC CAAAAACATC 
TCTCTACAGC TTTCTCAAAT GCAACTCGCC ATTGAAAGCA AACAAAGTGT TTCAATAGCT 
GCTGCAGTGA AGTTGATAAA ATTATCTTTC ACCTTTGAGG AATGGCATTT GTTTAATTCT 
GCTGCTTCTC AGTTTATCGG TTTTCTTCAG CTTCAAAGTG GCCCACACTT GCAGAAAGCC 
AGGAAGGATT TAATGCTTCT GGCTGCGATG GAACCGCTAA TTAATGTGAA GCGGAATAAA 
GGCTTAAGCT ATGCTGGTGA AGGAGGCAGC ACTACCACTG AAGGAGCTCG AAGCTCAGGG 
AGAAAAATTG TGTTTCAAGA AAATTTCTCA TTATCTGGAA GCTATTCTGA TGATATTTTC 
TATTTGGCAT CGATGCTGTT TTCCTTCATT TGTTCACCTA CTGAGAAAAT TCGTCCTGAT 
GAAGAGATCG TTGTTGACCT GATAATGTTC CTGTGGCAGA AATGCAAAAT GGGAATTCAG 
AAGATCTATT CAGCAAGAAT GGATTATTTC AAGTTTGTCC AGAAAGTCAA AACTAACAAA 
TGGATTCAGC TGCTTTGGCA GATAAATGAA ATGATTAATA CTTGTCAACC AGAAGACCTC 
AACCCTGTGT TTGTAGCTGA AGTTGCATTA CGACTAAGTG AAATACTGGA GATTTTAGGA 
AAGCCAGAAA AAAAAAGTCC TGGCAGCGCA AGCAGAGTAA CTATAGAATT CCAGCAACTT 
CCTGACAGTT TTCCACTATT TAAGAAAACA CCTAAAGAGC AGTTACTTTT GGCTCATGAA 
ACTCTTGAGA AGGCAGTTAC TGTGATGGGG CTATGTCGTT CCCAAACTAC ATTGCCTAAT 
GGAAAATCAG TATATGACCA TTGTCATATC AAGTTTATCC CTCCAAGAGG AGGTGGAACC 
ACTGGCAAAC CAATCACTGA CAATACTTTC CTGATGGACT TGCATCTAGA ACTCCTTGTG 
AGTCAGCACC GGATAGCTGC TACACTCATT GATCAACTCA AAGCTTGGAA TTTTTCTCCA 
AAACCTGCCC ATCCCAAAGA AACTGAAACT ACAACTACTA GTCATTTCAC AGAAGCATAC 
GTACTGGGCA AAATCAATAA GAATGAGTTA TCCAAAGCAG TCTTCTTAAT GATGAAAGCT 
CAGATACTAT TTGAGAAAGA AGATTCTCAG GTCATCAAAT CACTGGAGGA ATCGTTGTTC 
TTAATTCGGA AGATTGAATC AGAGCAAAAA AACCAGTATT CACCTCAGCA GAGTACAGAA 
GAAGAAAAAC CCCAGAGCAA AGTTCCTGCT CCTCCCATAC TACTTTCAAG AACTCATTGT 
TCAGTGACAT TTAAACCTGA TTCATTTTTT TGTGGAGAAA AGGTCGCTTG GTATGTCATT 
TTGGGACGTC CAGCAGGAAC ATGCATGAGA AAAGTAAGAT TGAACGACCA TAGTCTTCTA 
AACTGTGGAG AACCAGTACC TGCTACTAGT AAAACCGTTT TAGAAGTTGT TGGATTGGAG 
CCTAATGAAA AATACGTGTT TGCACTTGCT GGTTATTGCT CTGCTGGAAC ACTTATTGGG 
CAAGGAATTG GACTGACATC TAAGCCTATT CTGATTTACA TGCCACTTTC TACTGTTACT 
ATTCGCTTAC ATCTGACTGA GATTGCCTTT CAACTCGGTA AATATTCAGT GGCCAAGAAA 
GCCTTTGCAC CTGTTTGGGA CTATTTCGTC GATCCTCCTC TTCCAGAAAA TCCACACATT 
TTTTCTTTAA GTAGCTTGTT AACTGTCACA CCATTCAGGT TACATCTGGA GACCGTGTCA 
GAGTCTTCAT CAATCCTCTT ATACTATTTC ATTAGATGCA TTTTCATAAT AAGTAATATT 
AATATTATAG AAAAAAAGCT CTTCTGTGAT ACTATTAGAG GAGATGAGGT TTTCACTTGT 
CAACAAGTTT TCAGACTACT TGAATGTCAG AGAATCCTTG TGGTCATTGA ACTTAGTACC 
CATGTAAATA ATTTTAGTTA TTCCCTACAA GCTGTTGTTC ACTGCTATGG TCTCCTGGCC 
CCTCTAATCT TTCACAATAT TGTCCTGGTT CCAATAATCC AGGTCCTAAT TAAGTGTTTG 
GTAATTTTGC AAGAAATCCC AGGTGTTACC TTTCCAAAGA GACAGATGGG GAATTTTGAG 
TCTACCCAGC ATATGATTGC GTGCACTGTT TATTACACGG CAAAGGTGTT ACGTTCATGG 
AAGGAATTTG ACCTTGCAGT GATTATTATA AATTTGGGGA AAAAGTTACT GGACACCTCA 
CAAGGGTTAT TTTCAAATGT CACTAAAGCT GCCTTTGGTG AGGATGATCA AGATGACTTA 
TCAGAGGAGG CCTACTCAAC CAAGAGAATC AAGAGGGTCA AGGGACAGAT TGCCATACTG 
GACAAAATCA GCGAGCAGCT CAACGTCATG GAAGCTCAAC TACTCAAACT GACGAAACAA 
AGTCCCATGT ATGAGCTCAC AGGAACAGAA GATCCCTTTT TCCTCTACCC AATAATTCTA 
ACTTGGACTC TCAAAACTGC GATGAGGGAA GTAATGAAAT TCAAGAGTAA ACCTCGTTTT 
CTGGAATTCT TTGTTCAAGT TATGACAAAA TGTCTTATTG AAGAGAAATT TCACTATGTG 
GTGGAATTGG CATTTTCAGT TCAACTCTTC CTTAAAAGGA GAAATGCCAG TTTACTTGGA 
GTAAGGAGAT CTCCAGTTCT TCACACTCTT CCATCTAATG AAGAACCAAA GAAATACAAG 
GCAGCTGCCA TGGAGATTCA AAAAACTACA GATGTATCAA TTGGGAAGTC AAAATCAAAG 
AAAAAAGGTA TTCTGCAAGA TTATTTTGTC AAGCATCTCT CACTTTCTGA ATTGCCAGAT 
TTGGAAAAAT TTAAAAGAGC TGATGTGAGA AAAGTAGCTA TAAAAATCCT TTCAGAACAC 
TTGGCCCCAA AACTAAATCG CTATATAAAA AGGAAAAGAA TGCATCGAAT ATTTATTGAA 
GAGTTACCCT GGAAGTCTCA GATGAATGTA CATTTGGCTG AAGCTCACTT CAACTTATTC 
AAAATCAGAT TCATGGAGCG TAAGAAGGTT AAAGCTTGCA CTTCACAAAA TACAATGAGC 
TACCGAATTT TAGACCCGGA TATCTTCTCA ATGTACAATT CTGGGACGTT GTTAGTGGAT 
GAAAACTTAA ATTCAGATAA TTACCAAGCA TTGCTTGATT TCATGCTTTC AAGAAAAATG 
AGAAAGATCA ATCTCTCAGA TGCCAATGAA ATTTCTTTTG GTGGTGTCAG ATTGAGTGAA 
GATATCCCTA AAGGGGTAAA CACAAATGAC CTGGAATCCG CTAGAGAGAA AACAGCAAAT 
CTTGGAATCA TAGACCATTT TATCAGGACC TTTGTTCACT GCAAAAGAGC ATTGGTTCTT 
GCTCATCGTG GTGGTTTCTG GACCTTACTT CAGAATGTGA CCCGGACTCT TTGGAATTAC 
ACCATAGAGA TGCAGTTACT CTATAAACAG GCGCAGTTTC TTTCCAAAAC ATTCCCTTTC 
AGCAAGGATA TTATGATATG CACCTTCGTG ATGCCATTTA CTTTGGGAGC AGATGCCCTT 
ATTGACATGC TGTTAGACCT GCAGAATACC AAATCTCTTA AGATTGTGGA TGACTGTAAA 
GATTTTGGTG TTGCAAGTGC TTATGGGAAC ATCACAGAGG ATAATGGTGG CTTCAATCTG 
ACATTTGAAG AACCTTTTGA TGATATGAAT TTGGTTGACT TGAAATTCAT TTCTGACTTC 
ATCTTTAAGA CTTTGGAAAT CTTGTACAGA GTGGGAAAAT GGGAAACACT AGTCTACATT 
GCCCTTCAGT TCAATGAAGT GACACATGAG AGATACACTG AGCAAGTGAT GCCATTGTTA 
ATCTATTCTC AAAGACAGCT TTTGCAGAGA CTCCAGAGAA ATAGTGGGGA GCAGACAGAT 
AATATAGAAT GGCAGTTTGA TGACACGGTA AACCTCCGAG ATGTCATTGG GAAACTGCCC 
AATTCTTCTT TTGTGCAGAC TATAAAGGGC AAGTCTAAAA GACTTTTTAA TTATGAGCAA 
GGTGTAGATC TTTCGGAGAC ATACATTGCA AATAGAATAG TGTCTGTGCC CCTGAATGTG 
AAAGAAACTT TGAAGACCTT TAGAGAAACC TTGACGAAAT CTAAGTACCA CAACAGATCA 
CTGAGATACA GCAGAAAATT GCTTTCTTTG TTACTGGCCC ACACACAAGA AGCCAGTGGA 
GGAATAAACA ATCCCAAATT TATGTATGGA AAAGTGGAAT TTAGCCTTGG AACTGAAGAA 
ATACATTCGC CTACACCCCC TGATCTTTTT AATGAAAAGT TCACTGCTTC TGTTACAGTA 
CAGAGCACAA AAATTTCTCC TTTCCAATTT CAACTTGTTA TCACTTCTTA TGAGAAAACG 
ATCGAGATTC TTGAAATCAG CAATCAAACA GATCTCAAGA TACAGGCACT ACATGAGCTT 
GGAAATCTTC ACATCTATGC AGAAAACAGA AGGGCAGCTT TTAAATGCTG GAGTCAAGCT 
CTTGATGCCA TACTCAAAAA AAAAGATGTT CTACATACGT GGAAAGAAAT TGTCAGCCCC 
ATCCAAAAAC CTATGAATAG CATCGTGGGT TCAAACTACA TCGATTATAG TGAAGATTTT 
CTGTTGAAAG CTGGCATTTG GGGATGCTTG CAGGGAGGAG TCATAGCAGC AAAGATGGCC 
CAATACATTT TAACATCAAA TATGAATGAG AGGATTGAAT GTTGCATTTT CTCTTCATTT 
TTTTTTAAGG GTCTACTTAG AACCTCTCTT GTCCATCCCA AATATGACCA TGAGTATGCC 
CGTTATGAAA TCACCCAACT TACTCCAGGG CTGGATCTCT TCTGTGACCG ATACAGAGCA 
GATGTGTCCA TTGTCCTCAG CAGCCTGAGT TACCTCTGTC ATGAACTCCA TTCTGTCCAC 
CAAGACCTTA TTCTTCTGCC ACTTATTTCA TTAAACCATT ACTTGGTCTG TGAAGTTTGC 
CAAGATCTAT TTAAAACCAT AGATACAAGG ATCCTTAAGA CGGAAATCCT TATTGATTTA 
GGATTCTTTT CTGAAGCTTT TAGTGAAATG GCTGGTCTTT ATACTGGAAA AAACATCCCC 
ACTTCCACGC CCTTAGGCTA TAAACTTCCA AAAACTAAGA TCATGCAGCC CTTTCAAGCA 
GGACACTCTT ATTTGAGCAA AGAAAATATA CAGGCCCTGG AAGATTTAAT GAATCAAGGC 
ATCCCCCTAT ACATTATTTC GATTTGCCAA CCATCTTTCT CCTATAAGTT TACTTTGGTC 
AAAATGCGCT TTTTAATCAG TTTGGCTGCA ACCATAAATT GCTTCCCAGT TAGGGATACA 
ACAGCATTCT TCCAGGACTT TACCCAGAAG AGCAAAGCCA GTGCTTCAGT CTTTAAAGGT 
ACAAACCCTG AAGATTCAAT AATCCCCTCT CAAGAGACAG TTCAAAATGA CTTTACCGCA 
GCTGAAGGAA CACAGAGAGC TCTGGACAAG AGCATTGACT TCTCAAAGCA AAGAGGCTTC 
GATGATATGA GGAAGATGCT GATGGTGGAC CCAGAATATT TAAAGGATGA AACCCCAGCA 
AATGTTAACC TGACCAAGTC AAAAGAAGAG TTGTCTCCAG GTAAACTAAA GGGGATTTTA 
TTGGCAGAAG CTGAGGACAA GCTTCTCATG CTAATAGAAA ATGCAGAGGA ATTGAGTCAC 
AAGAAGATAA CTCAGTTTAG CCCAATAGAA TTGGAGATTA TGATAGAAGT CAAGTTACAG 
CTAGCCGCTA TTGCGATGCA GAGACAACAA GCCCCACTGA GCACTTCAAT AGCTTTCACT 
GCAATTAAAA TCCTTCAGGA TGCAGACATT TTCAAGAAGA CTAAAGAAGA ACTGTGTGCG 
AATGATGATG AACTGGATGC CATGGAGTCT GTGGAGATGT TGGCCCGAGA ACGTCTAAAC 
ATCCATCTAT GGCTAAAGTG TCGTCTGGCC TTGCTGGTGG CCACTGTGAC CCACATCCGT 
GGCATGGGGG TCGTGAAAGA AAATGAGATG ATTGAGTGCA GACTTTTGAT AAATGAAGTA 
TTTGTGGAGG CTCAAAACTG GAATGATACT GAGATGCAAG CAGAAGTCAT GGTGCAGGCT 
GTCAAGTTTG ACCTGCAAGA AAGACATTCT GCAGCCAACA TCTTAGAACA TTTGAAGGAT 
ATAAGAGGCT TACTTGAAGG AAAATCCTTT GTCTCTCCTC GAGGCTCTTT AATACTTATG 
AAAAGCCTGC TGTTAATGGA TGATTTAATG AAAATTGAGA TGCCCCAGGA ATATCTCTTC 
CTTAAAAATG AAAATCTCAA TTTACTAAAT CGAGCTCATG AGCTTATGAT TGCGCAGGCT 
CTATCTTTGG GTGAAAACAT TTTATTGCCT TGTTCAGACA CTGAATATGC AAATATCTTC 
TACCCTTTGA AAAACGTTTA TTTTCCATAC ATCAACTTAC TTGCAAAAAC AAAAATGAGG 
ATTGGGCATA TGTTAGCCAA GAGCACTGCT GCCTGTGAGA GAAAAGAACC TCAGAAGTGG 
GAACCCATTC GTCATCTCTT TGAGACAGCA TTGCAACTTT GTAGGGCAGC TGCAGTGAAG 
GAACGGGAGT TAGAAGCCGA AATCCTTTTT GAGAAAGGCA AAGTAGAACG TCAAATGCTT 
GTTGTACAAG ACAACAAGCC TTTAATTGGT GATCTTTTTG ATGCTATAAA AATAAGTCTA 
CAAAATGATG AAAATTCAGG GTTGATAAGG AAATCCTATC TGGAAATCGC CCTGTTATAT 
TTACTTGTGT GGAATTATAG GAAAAATGCT TCAAAAAATA AGATCTTTCT CCAAGTCCAG 
AATCCATCAA AGGAATCACT AGAAATTAAA ACTGAAATTT ACCTGGCAAT GTCCTGGATA 
GCAATCCGAG CTGCTGCACA GGTCAGTGAA GCTACAATGG CAGCTAAGAA GCTAACTGGA 
GAAAAGAGTG TGAAGACCAA GAAAATGGAT CAAAATGTCT ATCAGAATAT CCCTGAATTT 
GCTCATGCTG ACCTCACTTG TACCTATGTA GATTACTTGA CTGCTAACTA TAAAACTTGT 
GTCAAAACTT CCTATACCAC TCCGAGCGAA TCTGGAAGTT ATTTAAGTAT GGAACAAGAT 
CTGTACACCA ACCCCAGTGA AGAAAATGAG GAGCCCAGCC AGCTGGAGAT CACCTGGGTC 
CATCTGGCCC GTTACTACAC TCACCTGTTA AGAGTTAACA ATATGAATAC CTTGCTTGCT 
CCTCCAAAGC CAGTTTTCTT AACAGATGAA ATTTTCTACA CTTCTGTTTT CAATGAAGAT 
CTGATTATAC GTCAGAAAGA GATGAAGAAT TTCCTCAAAA AATTCTTAAG TGTTTTTACT 
TCAGCCTGTC TAGAGTGTTT CCCCAAAGAC CTCCTTCTTG GAATAGAAAG ACTAAATATT 
CCAGATAATG CCGAACTTAG GCAATCACTT AAGACATTTC AAGATGTGAG TGCTATAATA 
GAAAAAATAG TCACCACTAA GATCAACCCC ATTATCCTTC CTCCATCTCC CCCGCATGAA 
CATATGGATT TTGCTATACA TGCAGAAAGC CCAGATCTTT GCTTCCAGTG GTATATACCC 
CCTCTGGAAA AACCTCCAAG TGATAAAGAA CCCATGGTCA TGTTGCTGTA TGCCTATAAC 
ACCAAACCTG TCAAGATTAA TGATATCTTT GAATTCAATA TCTCCTCGAT ATTTCCTGGG 
TTTTTATGGC TTCCTCTGAA CAAAGTTATA GCCTTACATG AAGCTCTATC TGGCCTTAGG 
CAAGAAACTG AAATGTTGTT ATACACAAAA AAATTGCCTA CAGAAGAAAA GAAGTCCCTA 
CTACCCTTCA GTGCCTTAAA ACCTGCTAGA GAAGTCTCTG AAGAGATCGT CCTCAGTAAG 
CAAATGAAGA CTGTGTTTTA TCAGAGTTGC ACTGAAATAG CAAGACTGCT TTCGAGAAAT 
GAAGACATAC CCCTAATAAG TGAGGTTCCA TTTGAAGAAT CTTTGCCAAG TATACAGAAG 
TTGGAGATGC TTTTTGACCC CTCCAGTGGC TCTGTCTTAA AGGCAGAAGC CATTTTCAAC 
TGGATCGTGT CTTTTCTTAC ATCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281222.1
CDS	872..9961
Translation	MNVTTFKKVFFSKGVNDESAKLTFHALQGKCISQYQIICATDPCFKRELSVEQCFKILSVFRLMMQITLPYEHLCWIVYNGTIHIYTICRKLMTIGQSAKALEFLLWASICMESSLPLLAVSYLSWRATLYTAVCQCYYDCHAGMQGEIFARRGLGKIDDLLRLEVMSITPPQEEERKKFREATIKMAVMIFKRGVFEPRRKSKSYVRTKLRLNLREAQNLQWPRTATEKLLSEMFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_016425736.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425736.1

ORF Insert Sequence:

ATGAACGTGA CAACTTTCAA AAAAGTCTTT TTCTCCAAAG GTGTCAATGA TGAAAGCGCC 
AAACTTACAT TTCACGCTTT GCAAGGGAAA TGTATTAGCC AGTACCAAAT TATCTGTGCC 
ACCGATCCCT GTTTCAAGAG GGAGCTGTCT GTGGAGCAGT GCTTCAAGAT TCTGTCGGTT 
TTTCGGCTCA TGATGCAGAT AACCCTGCCC TACGAACACC TTTGCTGGAT CGTGTACAAT 
GGTACAATTC ACATTTATAC AATTTGCAGG AAGCTGATGA CCATTGGACA GTCTGCTAAG 
GCCTTGGAAT TCTTGCTCTG GGCCAGTATT TGTATGGAAT CCTCTTTACC CCTCTTAGCA 
GTAAGCTATC TCAGTTGGAG AGCTACCCTG TATACTGCTG TTTGCCAGTG CTACTATGAC 
TGCCATGCTG GAATGCAGGG AGAGATATTT GCTCGGCGAG GTTTGGGAAA AATTGATGAT 
TTATTACGTC TAGAAGTAAT GAGTATCACA CCACCACAGG AGGAAGAAAG AAAAAAATTC 
CGAGAGGCCA CCATCAAGAT GGCGGTGATG ATCTTTAAGA GAGGAGTCTT TGAACCACGA 
AGAAAGAGTA AGTCTTATGT AAGAACAAAA CTGAGACTGA ACCTACGGGA AGCACAGAAT 
TTGCAATGGC CACGCACAGC TACAGAGAAG TTGTTGAGTG AGATGTTTGA TTGTGGATCA 
GCCCAGTTTT TGGCTGTCTT GGAAGCCCTT TCTGAACCAA ATAGAAGAGT GTTACAAACT 
GGTCACTTGT CAGAGGAAAT GGAAGTTCGT GAGGTCATCG CTGAGCTCTT CATGGCAGCA 
AGCGAGCTTT TATCAGATGC ATGTAGTAGC CATGGGAAAT CCAGTTTTTC CAAAAACATC 
TCTCTACAGC TTTCTCAAAT GCAACTCGCC ATTGAAAGCA AACAAAGTGT TTCAATAGCT 
GCTGCAGTGA AGTTGATAAA ATTATCTTTC ACCTTTGAGG AATGGCATTT GTTTAATTCT 
GCTGCTTCTC AGTTTATCGG TTTTCTTCAG CTTCAAAGTG GCCCACACTT GCAGAAAGCC 
AGGAAGGATT TAATGCTTCT GGCTGCGATG GAACCGCTAA TTAATGTGAA GCGGAATAAA 
GGCTTAAGCT ATGCTGGTGA AGGAGGCAGC ACTACCACTG AAGGAGCTCG AAGCTCAGGG 
AGAAAAATTG TGTTTCAAGA AAATTTCTCA TTATCTGGAA GCTATTCTGA TGATATTTTC 
TATTTGGCAT CGATGCTGTT TTCCTTCATT TGTTCACCTA CTGAGAAAAT TCGTCCTGAT 
GAAGAGATCG TTGTTGACCT GATAATGTTC CTGTGGCAGA AATGCAAAAT GGGAATTCAG 
AAGATCTATT CAGCAAGAAT GGATTATTTC AAGTTTGTCC AGAAAGTCAA AACTAACAAA 
TGGATTCAGC TGCTTTGGCA GATAAATGAA ATGATTAATA CTTGTCAACC AGAAGACCTC 
AACCCTGTGT TTGTAGCTGA AGTTGCATTA CGACTAAGTG AAATACTGGA GATTTTAGGA 
AAGCCAGAAA AAAAAAGTCC TGGCAGCGCA AGCAGAGTAA CTATAGAATT CCAGCAACTT 
CCTGACAGTT TTCCACTATT TAAGAAAACA CCTAAAGAGC AGTTACTTTT GGCTCATGAA 
ACTCTTGAGA AGGCAGTTAC TGTGATGGGG CTATGTCGTT CCCAAACTAC ATTGCCTAAT 
GGAAAATCAG TATATGACCA TTGTCATATC AAGTTTATCC CTCCAAGAGG AGGTGGAACC 
ACTGGCAAAC CAATCACTGA CAATACTTTC CTGATGGACT TGCATCTAGA ACTCCTTGTG 
AGTCAGCACC GGATAGCTGC TACACTCATT GATCAACTCA AAGCTTGGAA TTTTTCTCCA 
AAACCTGCCC ATCCCAAAGA AACTGAAACT ACAACTACTA GTCATTTCAC AGAAGCATAC 
GTACTGGGCA AAATCAATAA GAATGAGTTA TCCAAAGCAG TCTTCTTAAT GATGAAAGCT 
CAGATACTAT TTGAGAAAGA AGATTCTCAG GTCATCAAAT CACTGGAGGA ATCGTTGTTC 
TTAATTCGGA AGATTGAATC AGAGCAAAAA AACCAGTATT CACCTCAGCA GAGTACAGAA 
GAAGAAAAAC CCCAGAGCAA AGTTCCTGCT CCTCCCATAC TACTTTCAAG AACTCATTGT 
TCAGTGACAT TTAAACCTGA TTCATTTTTT TGTGGAGAAA AGGTCGCTTG GTATGTCATT 
TTGGGACGTC CAGCAGGAAC ATGCATGAGA AAAGTAAGAT TGAACGACCA TAGTCTTCTA 
AACTGTGGAG AACCAGTACC TGCTACTAGT AAAACCGTTT TAGAAGTTGT TGGATTGGAG 
CCTAATGAAA AATACGTGTT TGCACTTGCT GGTTATTGCT CTGCTGGAAC ACTTATTGGG 
CAAGGAATTG GACTGACATC TAAGCCTATT CTGATTTACA TGCCACTTTC TACTGTTACT 
ATTCGCTTAC ATCTGACTGA GATTGCCTTT CAACTCGGTA AATATTCAGT GGCCAAGAAA 
GCCTTTGCAC CTGTTTGGGA CTATTTCGTC GATCCTCCTC TTCCAGAAAA TCCACACATT 
TTTTCTTTAA GTAGCTTGTT AACTGTCACA CCATTCAGGT TACATCTGGA GACCGTGTCA 
GAGTCTTCAT CAATCCTCTT ATACTATTTC ATTAGATGCA TTTTCATAAT AAGTAATATT 
AATATTATAG AAAAAAAGCT CTTCTGTGAT ACTATTAGAG GAGATGAGGT TTTCACTTGT 
CAACAAGTTT TCAGACTACT TGAATGTCAG AGAATCCTTG TGGTCATTGA ACTTAGTACC 
CATGTAAATA ATTTTAGTTA TTCCCTACAA GCTGTTGTTC ACTGCTATGG TCTCCTGGCC 
CCTCTAATCT TTCACAATAT TGTCCTGGTT CCAATAATCC AGGTCCTAAT TAAGTGTTTG 
GTAATTTTGC AAGAAATCCC AGGTGTTACC TTTCCAAAGA GACAGATGGG GAATTTTGAG 
TCTACCCAGC ATATGATTGC GTGCACTGTT TATTACACGG CAAAGGTGTT ACGTTCATGG 
AAGGAATTTG ACCTTGCAGT GATTATTATA AATTTGGGGA AAAAGTTACT GGACACCTCA 
CAAGGGTTAT TTTCAAATGT CACTAAAGCT GCCTTTGGTG AGGATGATCA AGATGACTTA 
TCAGAGGAGG CCTACTCAAC CAAGAGAATC AAGAGGGTCA AGGGACAGAT TGCCATACTG 
GACAAAATCA GCGAGCAGCT CAACGTCATG GAAGCTCAAC TACTCAAACT GACGAAACAA 
AGTCCCATGT ATGAGCTCAC AGGAACAGAA GATCCCTTTT TCCTCTACCC AATAATTCTA 
ACTTGGACTC TCAAAACTGC GATGAGGGAA GTAATGAAAT TCAAGAGTAA ACCTCGTTTT 
CTGGAATTCT TTGTTCAAGT TATGACAAAA TGTCTTATTG AAGAGAAATT TCACTATGTG 
GTGGAATTGG CATTTTCAGT TCAACTCTTC CTTAAAAGGA GAAATGCCAG TTTACTTGGA 
GTAAGGAGAT CTCCAGTTCT TCACACTCTT CCATCTAATG AAGAACCAAA GAAATACAAG 
GCAGCTGCCA TGGAGATTCA AAAAACTACA GATGTATCAA TTGGGAAGTC AAAATCAAAG 
AAAAAAGGTA TTCTGCAAGA TTATTTTGTC AAGCATCTCT CACTTTCTGA ATTGCCAGAT 
TTGGAAAAAT TTAAAAGAGC TGATGTGAGA AAAGTAGCTA TAAAAATCCT TTCAGAACAC 
TTGGCCCCAA AACTAAATCG CTATATAAAA AGGAAAAGAA TGCATCGAAT ATTTATTGAA 
GAGTTACCCT GGAAGTCTCA GATGAATGTA CATTTGGCTG AAGCTCACTT CAACTTATTC 
AAAATCAGAT TCATGGAGCG TAAGAAGGTT AAAGCTTGCA CTTCACAAAA TACAATGAGC 
TACCGAATTT TAGACCCGGA TATCTTCTCA ATGTACAATT CTGGGACGTT GTTAGTGGAT 
GAAAACTTAA ATTCAGATAA TTACCAAGCA TTGCTTGATT TCATGCTTTC AAGAAAAATG 
AGAAAGATCA ATCTCTCAGA TGCCAATGAA ATTTCTTTTG GTGGTGTCAG ATTGAGTGAA 
GATATCCCTA AAGGGGTAAA CACAAATGAC CTGGAATCCG CTAGAGAGAA AACAGCAAAT 
CTTGGAATCA TAGACCATTT TATCAGGACC TTTGTTCACT GCAAAAGAGC ATTGGTTCTT 
GCTCATCGTG GTGGTTTCTG GACCTTACTT CAGAATGTGA CCCGGACTCT TTGGAATTAC 
ACCATAGAGA TGCAGTTACT CTATAAACAG GCGCAGTTTC TTTCCAAAAC ATTCCCTTTC 
AGCAAGGATA TTATGATATG CACCTTCGTG ATGCCATTTA CTTTGGGAGC AGATGCCCTT 
ATTGACATGC TGTTAGACCT GCAGAATACC AAATCTCTTA AGATTGTGGA TGACTGTAAA 
GATTTTGGTG TTGCAAGTGC TTATGGGAAC ATCACAGAGG ATAATGGTGG CTTCAATCTG 
ACATTTGAAG AACCTTTTGA TGATATGAAT TTGGTTGACT TGAAATTCAT TTCTGACTTC 
ATCTTTAAGA CTTTGGAAAT CTTGTACAGA GTGGGAAAAT GGGAAACACT AGTCTACATT 
GCCCTTCAGT TCAATGAAGT GACACATGAG AGATACACTG AGCAAGTGAT GCCATTGTTA 
ATCTATTCTC AAAGACAGCT TTTGCAGAGA CTCCAGAGAA ATAGTGGGGA GCAGACAGAT 
AATATAGAAT GGCAGTTTGA TGACACGGTA AACCTCCGAG ATGTCATTGG GAAACTGCCC 
AATTCTTCTT TTGTGCAGAC TATAAAGGGC AAGTCTAAAA GACTTTTTAA TTATGAGCAA 
GGTGTAGATC TTTCGGAGAC ATACATTGCA AATAGAATAG TGTCTGTGCC CCTGAATGTG 
AAAGAAACTT TGAAGACCTT TAGAGAAACC TTGACGAAAT CTAAGTACCA CAACAGATCA 
CTGAGATACA GCAGAAAATT GCTTTCTTTG TTACTGGCCC ACACACAAGA AGCCAGTGGA 
GGAATAAACA ATCCCAAATT TATGTATGGA AAAGTGGAAT TTAGCCTTGG AACTGAAGAA 
ATACATTCGC CTACACCCCC TGATCTTTTT AATGAAAAGT TCACTGCTTC TGTTACAGTA 
CAGAGCACAA AAATTTCTCC TTTCCAATTT CAACTTGTTA TCACTTCTTA TGAGAAAACG 
ATCGAGATTC TTGAAATCAG CAATCAAACA GATCTCAAGA TACAGGCACT ACATGAGCTT 
GGAAATCTTC ACATCTATGC AGAAAACAGA AGGGCAGCTT TTAAATGCTG GAGTCAAGCT 
CTTGATGCCA TACTCAAAAA AAAAGATGTT CTACATACGT GGAAAGAAAT TGTCAGCCCC 
ATCCAAAAAC CTATGAATAG CATCGTGGGT TCAAACTACA TCGATTATAG TGAAGATTTT 
CTGTTGAAAG CTGGCATTTG GGGATGCTTG CAGGGAGGAG TCATAGCAGC AAAGATGGCC 
CAATACATTT TAACATCAAA TATGAATGAG AGGATTGAAT GTTGCATTTT CTCTTCATTT 
TTTTTTAAGG GTCTACTTAG AACCTCTCTT GTCCATCCCA AATATGACCA TGAGTATGCC 
CGTTATGAAA TCACCCAACT TACTCCAGGG CTGGATCTCT TCTGTGACCG ATACAGAGCA 
GATGTGTCCA TTGTCCTCAG CAGCCTGAGT TACCTCTGTC ATGAACTCCA TTCTGTCCAC 
CAAGACCTTA TTCTTCTGCC ACTTATTTCA TTAAACCATT ACTTGGTCTG TGAAGTTTGC 
CAAGATCTAT TTAAAACCAT AGATACAAGG ATCCTTAAGA CGGAAATCCT TATTGATTTA 
GGATTCTTTT CTGAAGCTTT TAGTGAAATG GCTGGTCTTT ATACTGGAAA AAACATCCCC 
ACTTCCACGC CCTTAGGCTA TAAACTTCCA AAAACTAAGA TCATGCAGCC CTTTCAAGCA 
GGACACTCTT ATTTGAGCAA AGAAAATATA CAGGCCCTGG AAGATTTAAT GAATCAAGGC 
ATCCCCCTAT ACATTATTTC GATTTGCCAA CCATCTTTCT CCTATAAGTT TACTTTGGTC 
AAAATGCGCT TTTTAATCAG TTTGGCTGCA ACCATAAATT GCTTCCCAGT TAGGGATACA 
ACAGCATTCT TCCAGGACTT TACCCAGAAG AGCAAAGCCA GTGCTTCAGT CTTTAAAGGT 
ACAAACCCTG AAGATTCAAT AATCCCCTCT CAAGAGACAG TTCAAAATGA CTTTACCGCA 
GCTGAAGGAA CACAGAGAGC TCTGGACAAG AGCATTGACT TCTCAAAGCA AAGAGGCTTC 
GATGATATGA GGAAGATGCT GATGGTGGAC CCAGAATATT TAAAGGATGA AACCCCAGCA 
AATGTTAACC TGACCAAGTC AAAAGAAGAG TTGTCTCCAG GTAAACTAAA GGGGATTTTA 
TTGGCAGAAG CTGAGGACAA GCTTCTCATG CTAATAGAAA ATGCAGAGGA ATTGAGTCAC 
AAGAAGATAA CTCAGTTTAG CCCAATAGAA TTGGAGATTA TGATAGAAGT CAAGTTACAG 
CTAGCCGCTA TTGCGATGCA GAGACAACAA GCCCCACTGA GCACTTCAAT AGCTTTCACT 
GCAATTAAAA TCCTTCAGGA TGCAGACATT TTCAAGAAGA CTAAAGAAGA ACTGTGTGCG 
AATGATGATG AACTGGATGC CATGGAGTCT GTGGAGATGT TGGCCCGAGA ACGTCTAAAC 
ATCCATCTAT GGCTAAAGTG TCGTCTGGCC TTGCTGGTGG CCACTGTGAC CCACATCCGT 
GGCATGGGGG TCGTGAAAGA AAATGAGATG ATTGAGTGCA GACTTTTGAT AAATGAAGTA 
TTTGTGGAGG CTCAAAACTG GAATGATACT GAGATGCAAG CAGAAGTCAT GGTGCAGGCT 
GTCAAGTTTG ACCTGCAAGA AAGACATTCT GCAGCCAACA TCTTAGAACA TTTGAAGGAT 
ATAAGAGGCT TACTTGAAGG AAAATCCTTT GTCTCTCCTC GAGGCTCTTT AATACTTATG 
AAAAGCCTGC TGTTAATGGA TGATTTAATG AAAATTGAGA TGCCCCAGGA ATATCTCTTC 
CTTAAAAATG AAAATCTCAA TTTACTAAAT CGAGCTCATG AGCTTATGAT TGCGCAGGCT 
CTATCTTTGG GTGAAAACAT TTTATTGCCT TGTTCAGACA CTGAATATGC AAATATCTTC 
TACCCTTTGA AAAACGTTTA TTTTCCATAC ATCAACTTAC TTGCAAAAAC AAAAATGAGG 
ATTGGGCATA TGTTAGCCAA GAGCACTGCT GCCTGTGAGA GAAAAGAACC TCAGAAGTGG 
GAACCCATTC GTCATCTCTT TGAGACAGCA TTGCAACTTT GTAGGGCAGC TGCAGTGAAG 
GAACGGGAGT TAGAAGCCGA AATCCTTTTT GAGAAAGGCA AAGTAGAACG TCAAATGCTT 
GTTGTACAAG ACAACAAGCC TTTAATTGGT GATCTTTTTG ATGCTATAAA AATAAGTCTA 
CAAAATGATG AAAATTCAGG GTTGATAAGG AAATCCTATC TGGAAATCGC CCTGTTATAT 
TTACTTGTGT GGAATTATAG GAAAAATGCT TCAAAAAATA AGATCTTTCT CCAAGTCCAG 
AATCCATCAA AGGAATCACT AGAAATTAAA ACTGAAATTT ACCTGGCAAT GTCCTGGATA 
GCAATCCGAG CTGCTGCACA GGTCAGTGAA GCTACAATGG CAGCTAAGAA GCTAACTGGA 
GAAAAGAGTG TGAAGACCAA GAAAATGGAT CAAAATGTCT ATCAGAATAT CCCTGAATTT 
GCTCATGCTG ACCTCACTTG TACCTATGTA GATTACTTGA CTGCTAACTA TAAAACTTGT 
GTCAAAACTT CCTATACCAC TCCGAGCGAA TCTGGAAGTT ATTTAAGTAT GGAACAAGAT 
CTGTACACCA ACCCCAGTGA AGAAAATGAG GAGCCCAGCC AGCTGGAGAT CACCTGGGTC 
CATCTGGCCC GTTACTACAC TCACCTGTTA AGAGTTAACA ATATGAATAC CTTGCTTGCT 
CCTCCAAAGC CAGTTTTCTT AACAGATGAA ATTTTCTACA CTTCTGTTTT CAATGAAGAT 
CTGATTATAC GTCAGAAAGA GATGAAGAAT TTCCTCAAAA AATTCTTAAG TGTTTTTACT 
TCAGCCTGTC TAGAGTGTTT CCCCAAAGAC CTCCTTCTTG GAATAGAAAG ACTAAATATT 
CCAGATAATG CCGAACTTAG GCAATCACTT AAGACATTTC AAGATGTGAG TGCTATAATA 
GAAAAAATAG TCACCACTAA GATCAACCCC ATTATCCTTC CTCCATCTCC CCCGCATGAA 
CATATGGATT TTGCTATACA TGCAGAAAGC CCAGATCTTT GCTTCCAGTG GTATATACCC 
CCTCTGGAAA AACCTCCAAG TGATAAAGAA CCCATGGTCA TGTTGCTGTA TGCCTATAAC 
ACCAAACCTG TCAAGATTAA TGATATCTTT GAATTCAATA TCTCCTCGAT ATTTCCTGGG 
TTTTTATGGC TTCCTCTGAA CAAAGTTATA GCCTTACATG AAGCTCTATC TGGCCTTAGG 
CAAGAAACTG AAATGTTGTT ATACACAAAA AAATTGCCTA CAGAAGAAAA GAAGTCCCTA 
CTACCCTTCA GTGCCTTAAA ACCTGCTAGA GAAGTCTCTG AAGAGATCGT CCTCAGTAAG 
CAAATGAAGA CTGTGTTTTA TCAGAGTTGC ACTGAAATAG CAAGACTGCT TTCGAGAAAT 
GAAGACATAC CCCTAATAAG TGAGGTTCCA TTTGAAGAAT CTTTGCCAAG TATACAGAAG 
TTGGAGATGC TTTTTGACCC CTCCAGTGGC TCTGTCTTAA AGGCAGAAGC CATTTTCAAC 
TGGATCGTGT CTTTTCTTAC ATCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP54 ( XM_016425737.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281223.1 Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA.

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CloneID	OMc45046
Clone ID Related Accession (Same CDS sequence)	XM_016425737.1
Accession Version	XM_016425737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	cilia- and flagella-associated protein 54 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGATT GTGGATCAGC CCAGTTTTTG GCTGTCTTGG AAGCCCTTTC TGAACCAAAT 
AGAAGAGTGT TACAAACTGG TCACTTGTCA GAGGAAATGG AAGTTCGTGA GGTCATCGCT 
GAGCTCTTCA TGGCAGCAAG CGAGCTTTTA TCAGATGCAT GTAGTAGCCA TGGGAAATCC 
AGTTTTTCCA AAAACATCTC TCTACAGCTT TCTCAAATGC AACTCGCCAT TGAAAGCAAA 
CAAAGTGTTT CAATAGCTGC TGCAGTGAAG TTGATAAAAT TATCTTTCAC CTTTGAGGAA 
TGGCATTTGT TTAATTCTGC TGCTTCTCAG TTTATCGGTT TTCTTCAGCT TCAAAGTGGC 
CCACACTTGC AGAAAGCCAG GAAGGATTTA ATGCTTCTGG CTGCGATGGA ACCGCTAATT 
AATGTGAAGC GGAATAAAGG CTTAAGCTAT GCTGGTGAAG GAGGCAGCAC TACCACTGAA 
GGAGCTCGAA GCTCAGGGAG AAAAATTGTG TTTCAAGAAA ATTTCTCATT ATCTGGAAGC 
TATTCTGATG ATATTTTCTA TTTGGCATCG ATGCTGTTTT CCTTCATTTG TTCACCTACT 
GAGAAAATTC GTCCTGATGA AGAGATCGTT GTTGACCTGA TAATGTTCCT GTGGCAGAAA 
TGCAAAATGG GAATTCAGAA GATCTATTCA GCAAGAATGG ATTATTTCAA GTTTGTCCAG 
AAAGTCAAAA CTAACAAATG GATTCAGCTG CTTTGGCAGA TAAATGAAAT GATTAATACT 
TGTCAACCAG AAGACCTCAA CCCTGTGTTT GTAGCTGAAG TTGCATTACG ACTAAGTGAA 
ATACTGGAGA TTTTAGGAAA GCCAGAAAAA AAAAGTCCTG GCAGCGCAAG CAGAGTAACT 
ATAGAATTCC AGCAACTTCC TGACAGTTTT CCACTATTTA AGAAAACACC TAAAGAGCAG 
TTACTTTTGG CTCATGAAAC TCTTGAGAAG GCAGTTACTG TGATGGGGCT ATGTCGTTCC 
CAAACTACAT TGCCTAATGG AAAATCAGTA TATGACCATT GTCATATCAA GTTTATCCCT 
CCAAGAGGAG GTGGAACCAC TGGCAAACCA ATCACTGACA ATACTTTCCT GATGGACTTG 
CATCTAGAAC TCCTTGTGAG TCAGCACCGG ATAGCTGCTA CACTCATTGA TCAACTCAAA 
GCTTGGAATT TTTCTCCAAA ACCTGCCCAT CCCAAAGAAA CTGAAACTAC AACTACTAGT 
CATTTCACAG AAGCATACGT ACTGGGCAAA ATCAATAAGA ATGAGTTATC CAAAGCAGTC 
TTCTTAATGA TGAAAGCTCA GATACTATTT GAGAAAGAAG ATTCTCAGGT CATCAAATCA 
CTGGAGGAAT CGTTGTTCTT AATTCGGAAG ATTGAATCAG AGCAAAAAAA CCAGTATTCA 
CCTCAGCAGA GTACAGAAGA AGAAAAACCC CAGAGCAAAG TTCCTGCTCC TCCCATACTA 
CTTTCAAGAA CTCATTGTTC AGTGACATTT AAACCTGATT CATTTTTTTG TGGAGAAAAG 
GTCGCTTGGT ATGTCATTTT GGGACGTCCA GCAGGAACAT GCATGAGAAA AGTAAGATTG 
AACGACCATA GTCTTCTAAA CTGTGGAGAA CCAGTACCTG CTACTAGTAA AACCGTTTTA 
GAAGTTGTTG GATTGGAGCC TAATGAAAAA TACGTGTTTG CACTTGCTGG TTATTGCTCT 
GCTGGAACAC TTATTGGGCA AGGAATTGGA CTGACATCTA AGCCTATTCT GATTTACATG 
CCACTTTCTA CTGTTACTAT TCGCTTACAT CTGACTGAGA TTGCCTTTCA ACTCGGTAAA 
TATTCAGTGG CCAAGAAAGC CTTTGCACCT GTTTGGGACT ATTTCGTCGA TCCTCCTCTT 
CCAGAAAATC CACACATTTT TTCTTTAAGT AGCTTGTTAA CTGTCACACC ATTCAGGTTA 
CATCTGGAGA CCGTGTCAGA GTCTTCATCA ATCCTCTTAT ACTATTTCAT TAGATGCATT 
TTCATAATAA GTAATATTAA TATTATAGAA AAAAAGCTCT TCTGTGATAC TATTAGAGGA 
GATGAGGTTT TCACTTGTCA ACAAGTTTTC AGACTACTTG AATGTCAGAG AATCCTTGTG 
GTCATTGAAC TTAGTACCCA TGTAAATAAT TTTAGTTATT CCCTACAAGC TGTTGTTCAC 
TGCTATGGTC TCCTGGCCCC TCTAATCTTT CACAATATTG TCCTGGTTCC AATAATCCAG 
GTCCTAATTA AGTGTTTGGT AATTTTGCAA GAAATCCCAG GTGTTACCTT TCCAAAGAGA 
CAGATGGGGA ATTTTGAGTC TACCCAGCAT ATGATTGCGT GCACTGTTTA TTACACGGCA 
AAGGTGTTAC GTTCATGGAA GGAATTTGAC CTTGCAGTGA TTATTATAAA TTTGGGGAAA 
AAGTTACTGG ACACCTCACA AGGGTTATTT TCAAATGTCA CTAAAGCTGC CTTTGGTGAG 
GATGATCAAG ATGACTTATC AGAGGAGGCC TACTCAACCA AGAGAATCAA GAGGGTCAAG 
GGACAGATTG CCATACTGGA CAAAATCAGC GAGCAGCTCA ACGTCATGGA AGCTCAACTA 
CTCAAACTGA CGAAACAAAG TCCCATGTAT GAGCTCACAG GAACAGAAGA TCCCTTTTTC 
CTCTACCCAA TAATTCTAAC TTGGACTCTC AAAACTGCGA TGAGGGAAGT AATGAAATTC 
AAGAGTAAAC CTCGTTTTCT GGAATTCTTT GTTCAAGTTA TGACAAAATG TCTTATTGAA 
GAGAAATTTC ACTATGTGGT GGAATTGGCA TTTTCAGTTC AACTCTTCCT TAAAAGGAGA 
AATGCCAGTT TACTTGGAGT AAGGAGATCT CCAGTTCTTC ACACTCTTCC ATCTAATGAA 
GAACCAAAGA AATACAAGGC AGCTGCCATG GAGATTCAAA AAACTACAGA TGTATCAATT 
GGGAAGTCAA AATCAAAGAA AAAAGGTATT CTGCAAGATT ATTTTGTCAA GCATCTCTCA 
CTTTCTGAAT TGCCAGATTT GGAAAAATTT AAAAGAGCTG ATGTGAGAAA AGTAGCTATA 
AAAATCCTTT CAGAACACTT GGCCCCAAAA CTAAATCGCT ATATAAAAAG GAAAAGAATG 
CATCGAATAT TTATTGAAGA GTTACCCTGG AAGTCTCAGA TGAATGTACA TTTGGCTGAA 
GCTCACTTCA ACTTATTCAA AATCAGATTC ATGGAGCGTA AGAAGGTTAA AGCTTGCACT 
TCACAAAATA CAATGAGCTA CCGAATTTTA GACCCGGATA TCTTCTCAAT GTACAATTCT 
GGGACGTTGT TAGTGGATGA AAACTTAAAT TCAGATAATT ACCAAGCATT GCTTGATTTC 
ATGCTTTCAA GAAAAATGAG AAAGATCAAT CTCTCAGATG CCAATGAAAT TTCTTTTGGT 
GGTGTCAGAT TGAGTGAAGA TATCCCTAAA GGGGTAAACA CAAATGACCT GGAATCCGCT 
AGAGAGAAAA CAGCAAATCT TGGAATCATA GACCATTTTA TCAGGACCTT TGTTCACTGC 
AAAAGAGCAT TGGTTCTTGC TCATCGTGGT GGTTTCTGGA CCTTACTTCA GAATGTGACC 
CGGACTCTTT GGAATTACAC CATAGAGATG CAGTTACTCT ATAAACAGGC GCAGTTTCTT 
TCCAAAACAT TCCCTTTCAG CAAGGATATT ATGATATGCA CCTTCGTGAT GCCATTTACT 
TTGGGAGCAG ATGCCCTTAT TGACATGCTG TTAGACCTGC AGAATACCAA ATCTCTTAAG 
ATTGTGGATG ACTGTAAAGA TTTTGGTGTT GCAAGTGCTT ATGGGAACAT CACAGAGGAT 
AATGGTGGCT TCAATCTGAC ATTTGAAGAA CCTTTTGATG ATATGAATTT GGTTGACTTG 
AAATTCATTT CTGACTTCAT CTTTAAGACT TTGGAAATCT TGTACAGAGT GGGAAAATGG 
GAAACACTAG TCTACATTGC CCTTCAGTTC AATGAAGTGA CACATGAGAG ATACACTGAG 
CAAGTGATGC CATTGTTAAT CTATTCTCAA AGACAGCTTT TGCAGAGACT CCAGAGAAAT 
AGTGGGGAGC AGACAGATAA TATAGAATGG CAGTTTGATG ACACGGTAAA CCTCCGAGAT 
GTCATTGGGA AACTGCCCAA TTCTTCTTTT GTGCAGACTA TAAAGGGCAA GTCTAAAAGA 
CTTTTTAATT ATGAGCAAGG TGTAGATCTT TCGGAGACAT ACATTGCAAA TAGAATAGTG 
TCTGTGCCCC TGAATGTGAA AGAAACTTTG AAGACCTTTA GAGAAACCTT GACGAAATCT 
AAGTACCACA ACAGATCACT GAGATACAGC AGAAAATTGC TTTCTTTGTT ACTGGCCCAC 
ACACAAGAAG CCAGTGGAGG AATAAACAAT CCCAAATTTA TGTATGGAAA AGTGGAATTT 
AGCCTTGGAA CTGAAGAAAT ACATTCGCCT ACACCCCCTG ATCTTTTTAA TGAAAAGTTC 
ACTGCTTCTG TTACAGTACA GAGCACAAAA ATTTCTCCTT TCCAATTTCA ACTTGTTATC 
ACTTCTTATG AGAAAACGAT CGAGATTCTT GAAATCAGCA ATCAAACAGA TCTCAAGATA 
CAGGCACTAC ATGAGCTTGG AAATCTTCAC ATCTATGCAG AAAACAGAAG GGCAGCTTTT 
AAATGCTGGA GTCAAGCTCT TGATGCCATA CTCAAAAAAA AAGATGTTCT ACATACGTGG 
AAAGAAATTG TCAGCCCCAT CCAAAAACCT ATGAATAGCA TCGTGGGTTC AAACTACATC 
GATTATAGTG AAGATTTTCT GTTGAAAGCT GGCATTTGGG GATGCTTGCA GGGAGGAGTC 
ATAGCAGCAA AGATGGCCCA ATACATTTTA ACATCAAATA TGAATGAGAG GATTGAATGT 
TGCATTTTCT CTTCATTTTT TTTTAAGGGT CTACTTAGAA CCTCTCTTGT CCATCCCAAA 
TATGACCATG AGTATGCCCG TTATGAAATC ACCCAACTTA CTCCAGGGCT GGATCTCTTC 
TGTGACCGAT ACAGAGCAGA TGTGTCCATT GTCCTCAGCA GCCTGAGTTA CCTCTGTCAT 
GAACTCCATT CTGTCCACCA AGACCTTATT CTTCTGCCAC TTATTTCATT AAACCATTAC 
TTGGTCTGTG AAGTTTGCCA AGATCTATTT AAAACCATAG ATACAAGGAT CCTTAAGACG 
GAAATCCTTA TTGATTTAGG ATTCTTTTCT GAAGCTTTTA GTGAAATGGC TGGTCTTTAT 
ACTGGAAAAA ACATCCCCAC TTCCACGCCC TTAGGCTATA AACTTCCAAA AACTAAGATC 
ATGCAGCCCT TTCAAGCAGG ACACTCTTAT TTGAGCAAAG AAAATATACA GGCCCTGGAA 
GATTTAATGA ATCAAGGCAT CCCCCTATAC ATTATTTCGA TTTGCCAACC ATCTTTCTCC 
TATAAGTTTA CTTTGGTCAA AATGCGCTTT TTAATCAGTT TGGCTGCAAC CATAAATTGC 
TTCCCAGTTA GGGATACAAC AGCATTCTTC CAGGACTTTA CCCAGAAGAG CAAAGCCAGT 
GCTTCAGTCT TTAAAGGTAC AAACCCTGAA GATTCAATAA TCCCCTCTCA AGAGACAGTT 
CAAAATGACT TTACCGCAGC TGAAGGAACA CAGAGAGCTC TGGACAAGAG CATTGACTTC 
TCAAAGCAAA GAGGCTTCGA TGATATGAGG AAGATGCTGA TGGTGGACCC AGAATATTTA 
AAGGATGAAA CCCCAGCAAA TGTTAACCTG ACCAAGTCAA AAGAAGAGTT GTCTCCAGGT 
AAACTAAAGG GGATTTTATT GGCAGAAGCT GAGGACAAGC TTCTCATGCT AATAGAAAAT 
GCAGAGGAAT TGAGTCACAA GAAGATAACT CAGTTTAGCC CAATAGAATT GGAGATTATG 
ATAGAAGTCA AGTTACAGCT AGCCGCTATT GCGATGCAGA GACAACAAGC CCCACTGAGC 
ACTTCAATAG CTTTCACTGC AATTAAAATC CTTCAGGATG CAGACATTTT CAAGAAGACT 
AAAGAAGAAC TGTGTGCGAA TGATGATGAA CTGGATGCCA TGGAGTCTGT GGAGATGTTG 
GCCCGAGAAC GTCTAAACAT CCATCTATGG CTAAAGTGTC GTCTGGCCTT GCTGGTGGCC 
ACTGTGACCC ACATCCGTGG CATGGGGGTC GTGAAAGAAA ATGAGATGAT TGAGTGCAGA 
CTTTTGATAA ATGAAGTATT TGTGGAGGCT CAAAACTGGA ATGATACTGA GATGCAAGCA 
GAAGTCATGG TGCAGGCTGT CAAGTTTGAC CTGCAAGAAA GACATTCTGC AGCCAACATC 
TTAGAACATT TGAAGGATAT AAGAGGCTTA CTTGAAGGAA AATCCTTTGT CTCTCCTCGA 
GGCTCTTTAA TACTTATGAA AAGCCTGCTG TTAATGGATG ATTTAATGAA AATTGAGATG 
CCCCAGGAAT ATCTCTTCCT TAAAAATGAA AATCTCAATT TACTAAATCG AGCTCATGAG 
CTTATGATTG CGCAGGCTCT ATCTTTGGGT GAAAACATTT TATTGCCTTG TTCAGACACT 
GAATATGCAA ATATCTTCTA CCCTTTGAAA AACGTTTATT TTCCATACAT CAACTTACTT 
GCAAAAACAA AAATGAGGAT TGGGCATATG TTAGCCAAGA GCACTGCTGC CTGTGAGAGA 
AAAGAACCTC AGAAGTGGGA ACCCATTCGT CATCTCTTTG AGACAGCATT GCAACTTTGT 
AGGGCAGCTG CAGTGAAGGA ACGGGAGTTA GAAGCCGAAA TCCTTTTTGA GAAAGGCAAA 
GTAGAACGTC AAATGCTTGT TGTACAAGAC AACAAGCCTT TAATTGGTGA TCTTTTTGAT 
GCTATAAAAA TAAGTCTACA AAATGATGAA AATTCAGGGT TGATAAGGAA ATCCTATCTG 
GAAATCGCCC TGTTATATTT ACTTGTGTGG AATTATAGGA AAAATGCTTC AAAAAATAAG 
ATCTTTCTCC AAGTCCAGAA TCCATCAAAG GAATCACTAG AAATTAAAAC TGAAATTTAC 
CTGGCAATGT CCTGGATAGC AATCCGAGCT GCTGCACAGG TCAGTGAAGC TACAATGGCA 
GCTAAGAAGC TAACTGGAGA AAAGAGTGTG AAGACCAAGA AAATGGATCA AAATGTCTAT 
CAGAATATCC CTGAATTTGC TCATGCTGAC CTCACTTGTA CCTATGTAGA TTACTTGACT 
GCTAACTATA AAACTTGTGT CAAAACTTCC TATACCACTC CGAGCGAATC TGGAAGTTAT 
TTAAGTATGG AACAAGATCT GTACACCAAC CCCAGTGAAG AAAATGAGGA GCCCAGCCAG 
CTGGAGATCA CCTGGGTCCA TCTGGCCCGT TACTACACTC ACCTGTTAAG AGTTAACAAT 
ATGAATACCT TGCTTGCTCC TCCAAAGCCA GTTTTCTTAA CAGATGAAAT TTTCTACACT 
TCTGTTTTCA ATGAAGATCT GATTATACGT CAGAAAGAGA TGAAGAATTT CCTCAAAAAA 
TTCTTAAGTG TTTTTACTTC AGCCTGTCTA GAGTGTTTCC CCAAAGACCT CCTTCTTGGA 
ATAGAAAGAC TAAATATTCC AGATAATGCC GAACTTAGGC AATCACTTAA GACATTTCAA 
GATGTGAGTG CTATAATAGA AAAAATAGTC ACCACTAAGA TCAACCCCAT TATCCTTCCT 
CCATCTCCCC CGCATGAACA TATGGATTTT GCTATACATG CAGAAAGCCC AGATCTTTGC 
TTCCAGTGGT ATATACCCCC TCTGGAAAAA CCTCCAAGTG ATAAAGAACC CATGGTCATG 
TTGCTGTATG CCTATAACAC CAAACCTGTC AAGATTAATG ATATCTTTGA ATTCAATATC 
TCCTCGATAT TTCCTGGGTT TTTATGGCTT CCTCTGAACA AAGTTATAGC CTTACATGAA 
GCTCTATCTG GCCTTAGGCA AGAAACTGAA ATGTTGTTAT ACACAAAAAA ATTGCCTACA 
GAAGAAAAGA AGTCCCTACT ACCCTTCAGT GCCTTAAAAC CTGCTAGAGA AGTCTCTGAA 
GAGATCGTCC TCAGTAAGCA AATGAAGACT GTGTTTTATC AGAGTTGCAC TGAAATAGCA 
AGACTGCTTT CGAGAAATGA AGACATACCC CTAATAAGTG AGGTTCCATT TGAAGAATCT 
TTGCCAAGTA TACAGAAGTT GGAGATGCTT TTTGACCCCT CCAGTGGCTC TGTCTTAAAG 
GCAGAAGCCA TTTTCAACTG GATCGTGTCT TTTCTTACAT CTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281223.1
CDS	320..8707
Translation	MFDCGSAQFLAVLEALSEPNRRVLQTGHLSEEMEVREVIAELFMAASELLSDACSSHGKSSFSKNISLQLSQMQLAIESKQSVSIAAAVKLIKLSFTFEEWHLFNSAASQFIGFLQLQSGPHLQKARKDLMLLAAMEPLINVKRNKGLSYAGEGGSTTTEGARSSGRKIVFQENFSLSGSYSDDIFYLASMLFSFICSPTEKIRPDEEIVVDLIMFLWQKCKMGIQKIYSARMDYFKFVQKVKTNKWIQLLWQINEMINTCQPEDLNPVFVAEVALRLSEILEILGKPEKKSPGSASRVTIEFQQLPDSFPLFKKTPKEQLLLAHETLEKAVTVMGLCRSQTTLPNGKSVYDHCHIKFIPPRGGGTTGKPITDNTFLMDLHLELLVSQHRIAATLIDQLKAWNFSPKPAHPKETETTTTSHFTEAYVLGKINKNELSKAVFLMMKAQILFEKEDSQVIKSLEESLFLIRKIESEQKNQYSPQQSTEEEKPQSKVPAPPILLSRTHCSVTFKPDSFFCGEKVAWYVILGRPAGTCMRKVRLNDHSLLNCGEPVPATSKTVLEVVGLEPNEKYVFALAGYCSAGTLIGQGIGLTSKPILIYMPLSTVTIRLHLTEIAFQLGKYSVAKKAFAPVWDYFVDPPLPENPHIFSLSSLLTVTPFRLHLETVSESSSILLYYFIRCIFIISNINIIEKKLFCDTIRGDEVFTCQQVFRLLECQRILVVIELSTHVNNFSYSLQAVVHCYGLLAPLIFHNIVLVPIIQVLIKCLVILQEIPGVTFPKRQMGNFESTQHMIACTVYYTAKVLRSWKEFDLAVIIINLGKKLLDTSQGLFSNVTKAAFGEDDQDDLSEEAYSTKRIKRVKGQIAILDKISEQLNVMEAQLLKLTKQSPMYELTGTEDPFFLYPIILTWTLKTAMREVMKFKSKPRFLEFFVQVMTKCLIEEKFHYVVELAFSVQLFLKRRNASLLGVRRSPVLHTLPSNEEPKKYKAAAMEIQKTTDVSIGKSKSKKKGILQDYFVKHLSLSELPDLEKFKRADVRKVAIKILSEHLAPKLNRYIKRKRMHRIFIEELPWKSQMNVHLAEAHFNLFKIRFMERKKVKACTSQNTMSYRILDPDIFSMYNSGTLLVDENLNSDNYQALLDFMLSRKMRKINLSDANEISFGGVRLSEDIPKGVNTNDLESAREKTANLGIIDHFIRTFVHCKRALVLAHRGGFWTLLQNVTRTLWNYTIEMQLLYKQAQFLSKTFPFSKDIMICTFVMPFTLGADALIDMLLDLQNTKSLKIVDDCKDFGVASAYGNITEDNGGFNLTFEEPFDDMNLVDLKFISDFIFKTLEILYRVGKWETLVYIALQFNEVTHERYTEQVMPLLIYSQRQLLQRLQRNSGEQTDNIEWQFDDTVNLRDVIGKLPNSSFVQTIKGKSKRLFNYEQGVDLSETYIANRIVSVPLNVKETLKTFRETLTKSKYHNRSLRYSRKLLSLLLAHTQEASGGINNPKFMYGKVEFSLGTEEIHSPTPPDLFNEKFTASVTVQSTKISPFQFQLVITSYEKTIEILEISNQTDLKIQALHELGNLHIYAENRRAAFKCWSQALDAILKKKDVLHTWKEIVSPIQKPMNSIVGSNYIDYSEDFLLKAGIWGCLQGGVIAAKMAQYILTSNMNERIECCIFSSFFFKGLLRTSLVHPKYDHEYARYEITQLTPGLDLFCDRYRADVSIVLSSLSYLCHELHSVHQDLILLPLISLNHYLVCEVCQDLFKTIDTRILKTEILIDLGFFSEAFSEMAGLYTGKNIPTSTPLGYKLPKTKIMQPFQAGHSYLSKENIQALEDLMNQGIPLYIISICQPSFSYKFTLVKMRFLISLAATINCFPVRDTTAFFQDFTQKSKASASVFKGTNPEDSIIPSQETVQNDFTAAEGTQRALDKSIDFSKQRGFDDMRKMLMVDPEYLKDETPANVNLTKSKEELSPGKLKGILLAEAEDKLLMLIENAEELSHKKITQFSPIELEIMIEVKLQLAAIAMQRQQAPLSTSIAFTAIKILQDADIFKKTKEELCANDDELDAMESVEMLARERLNIHLWLKCRLALLVATVTHIRGMGVVKENEMIECRLLINEVFVEAQNWNDTEMQAEVMVQAVKFDLQERHSAANILEHLKDIRGLLEGKSFVSPRGSLILMKSLLLMDDLMKIEMPQEYLFLKNENLNLLNRAHELMIAQALSLGENILLPCSDTEYANIFYPLKNVYFPYINLLAKTKMRIGHMLAKSTAACERKEPQKWEPIRHLFETALQLCRAAAVKERELEAEILFEKGKVERQMLVVQDNKPLIGDLFDAIKISLQNDENSGLIRKSYLEIALLYLLVWNYRKNASKNKIFLQVQNPSKESLEIKTEIYLAMSWIAIRAAAQVSEATMAAKKLTGEKSVKTKKMDQNVYQNIPEFAHADLTCTYVDYLTANYKTCVKTSYTTPSESGSYLSMEQDLYTNPSEENEEPSQLEITWVHLARYYTHLLRVNNMNTLLAPPKPVFLTDEIFYTSVFNEDLIIRQKEMKNFLKKFLSVFTSACLECFPKDLLLGIERLNIPDNAELRQSLKTFQDVSAIIEKIVTTKINPIILPPSPPHEHMDFAIHAESPDLCFQWYIPPLEKPPSDKEPMVMLLYAYNTKPVKINDIFEFNISSIFPGFLWLPLNKVIALHEALSGLRQETEMLLYTKKLPTEEKKSLLPFSALKPAREVSEEIVLSKQMKTVFYQSCTEIARLLSRNEDIPLISEVPFEESLPSIQKLEMLFDPSSGSVLKAEAIFNWIVSFLTSL

Target ORF information:

RefSeq Version	XM_016425737.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425737.1

ORF Insert Sequence:

ATGTTTGATT GTGGATCAGC CCAGTTTTTG GCTGTCTTGG AAGCCCTTTC TGAACCAAAT 
AGAAGAGTGT TACAAACTGG TCACTTGTCA GAGGAAATGG AAGTTCGTGA GGTCATCGCT 
GAGCTCTTCA TGGCAGCAAG CGAGCTTTTA TCAGATGCAT GTAGTAGCCA TGGGAAATCC 
AGTTTTTCCA AAAACATCTC TCTACAGCTT TCTCAAATGC AACTCGCCAT TGAAAGCAAA 
CAAAGTGTTT CAATAGCTGC TGCAGTGAAG TTGATAAAAT TATCTTTCAC CTTTGAGGAA 
TGGCATTTGT TTAATTCTGC TGCTTCTCAG TTTATCGGTT TTCTTCAGCT TCAAAGTGGC 
CCACACTTGC AGAAAGCCAG GAAGGATTTA ATGCTTCTGG CTGCGATGGA ACCGCTAATT 
AATGTGAAGC GGAATAAAGG CTTAAGCTAT GCTGGTGAAG GAGGCAGCAC TACCACTGAA 
GGAGCTCGAA GCTCAGGGAG AAAAATTGTG TTTCAAGAAA ATTTCTCATT ATCTGGAAGC 
TATTCTGATG ATATTTTCTA TTTGGCATCG ATGCTGTTTT CCTTCATTTG TTCACCTACT 
GAGAAAATTC GTCCTGATGA AGAGATCGTT GTTGACCTGA TAATGTTCCT GTGGCAGAAA 
TGCAAAATGG GAATTCAGAA GATCTATTCA GCAAGAATGG ATTATTTCAA GTTTGTCCAG 
AAAGTCAAAA CTAACAAATG GATTCAGCTG CTTTGGCAGA TAAATGAAAT GATTAATACT 
TGTCAACCAG AAGACCTCAA CCCTGTGTTT GTAGCTGAAG TTGCATTACG ACTAAGTGAA 
ATACTGGAGA TTTTAGGAAA GCCAGAAAAA AAAAGTCCTG GCAGCGCAAG CAGAGTAACT 
ATAGAATTCC AGCAACTTCC TGACAGTTTT CCACTATTTA AGAAAACACC TAAAGAGCAG 
TTACTTTTGG CTCATGAAAC TCTTGAGAAG GCAGTTACTG TGATGGGGCT ATGTCGTTCC 
CAAACTACAT TGCCTAATGG AAAATCAGTA TATGACCATT GTCATATCAA GTTTATCCCT 
CCAAGAGGAG GTGGAACCAC TGGCAAACCA ATCACTGACA ATACTTTCCT GATGGACTTG 
CATCTAGAAC TCCTTGTGAG TCAGCACCGG ATAGCTGCTA CACTCATTGA TCAACTCAAA 
GCTTGGAATT TTTCTCCAAA ACCTGCCCAT CCCAAAGAAA CTGAAACTAC AACTACTAGT 
CATTTCACAG AAGCATACGT ACTGGGCAAA ATCAATAAGA ATGAGTTATC CAAAGCAGTC 
TTCTTAATGA TGAAAGCTCA GATACTATTT GAGAAAGAAG ATTCTCAGGT CATCAAATCA 
CTGGAGGAAT CGTTGTTCTT AATTCGGAAG ATTGAATCAG AGCAAAAAAA CCAGTATTCA 
CCTCAGCAGA GTACAGAAGA AGAAAAACCC CAGAGCAAAG TTCCTGCTCC TCCCATACTA 
CTTTCAAGAA CTCATTGTTC AGTGACATTT AAACCTGATT CATTTTTTTG TGGAGAAAAG 
GTCGCTTGGT ATGTCATTTT GGGACGTCCA GCAGGAACAT GCATGAGAAA AGTAAGATTG 
AACGACCATA GTCTTCTAAA CTGTGGAGAA CCAGTACCTG CTACTAGTAA AACCGTTTTA 
GAAGTTGTTG GATTGGAGCC TAATGAAAAA TACGTGTTTG CACTTGCTGG TTATTGCTCT 
GCTGGAACAC TTATTGGGCA AGGAATTGGA CTGACATCTA AGCCTATTCT GATTTACATG 
CCACTTTCTA CTGTTACTAT TCGCTTACAT CTGACTGAGA TTGCCTTTCA ACTCGGTAAA 
TATTCAGTGG CCAAGAAAGC CTTTGCACCT GTTTGGGACT ATTTCGTCGA TCCTCCTCTT 
CCAGAAAATC CACACATTTT TTCTTTAAGT AGCTTGTTAA CTGTCACACC ATTCAGGTTA 
CATCTGGAGA CCGTGTCAGA GTCTTCATCA ATCCTCTTAT ACTATTTCAT TAGATGCATT 
TTCATAATAA GTAATATTAA TATTATAGAA AAAAAGCTCT TCTGTGATAC TATTAGAGGA 
GATGAGGTTT TCACTTGTCA ACAAGTTTTC AGACTACTTG AATGTCAGAG AATCCTTGTG 
GTCATTGAAC TTAGTACCCA TGTAAATAAT TTTAGTTATT CCCTACAAGC TGTTGTTCAC 
TGCTATGGTC TCCTGGCCCC TCTAATCTTT CACAATATTG TCCTGGTTCC AATAATCCAG 
GTCCTAATTA AGTGTTTGGT AATTTTGCAA GAAATCCCAG GTGTTACCTT TCCAAAGAGA 
CAGATGGGGA ATTTTGAGTC TACCCAGCAT ATGATTGCGT GCACTGTTTA TTACACGGCA 
AAGGTGTTAC GTTCATGGAA GGAATTTGAC CTTGCAGTGA TTATTATAAA TTTGGGGAAA 
AAGTTACTGG ACACCTCACA AGGGTTATTT TCAAATGTCA CTAAAGCTGC CTTTGGTGAG 
GATGATCAAG ATGACTTATC AGAGGAGGCC TACTCAACCA AGAGAATCAA GAGGGTCAAG 
GGACAGATTG CCATACTGGA CAAAATCAGC GAGCAGCTCA ACGTCATGGA AGCTCAACTA 
CTCAAACTGA CGAAACAAAG TCCCATGTAT GAGCTCACAG GAACAGAAGA TCCCTTTTTC 
CTCTACCCAA TAATTCTAAC TTGGACTCTC AAAACTGCGA TGAGGGAAGT AATGAAATTC 
AAGAGTAAAC CTCGTTTTCT GGAATTCTTT GTTCAAGTTA TGACAAAATG TCTTATTGAA 
GAGAAATTTC ACTATGTGGT GGAATTGGCA TTTTCAGTTC AACTCTTCCT TAAAAGGAGA 
AATGCCAGTT TACTTGGAGT AAGGAGATCT CCAGTTCTTC ACACTCTTCC ATCTAATGAA 
GAACCAAAGA AATACAAGGC AGCTGCCATG GAGATTCAAA AAACTACAGA TGTATCAATT 
GGGAAGTCAA AATCAAAGAA AAAAGGTATT CTGCAAGATT ATTTTGTCAA GCATCTCTCA 
CTTTCTGAAT TGCCAGATTT GGAAAAATTT AAAAGAGCTG ATGTGAGAAA AGTAGCTATA 
AAAATCCTTT CAGAACACTT GGCCCCAAAA CTAAATCGCT ATATAAAAAG GAAAAGAATG 
CATCGAATAT TTATTGAAGA GTTACCCTGG AAGTCTCAGA TGAATGTACA TTTGGCTGAA 
GCTCACTTCA ACTTATTCAA AATCAGATTC ATGGAGCGTA AGAAGGTTAA AGCTTGCACT 
TCACAAAATA CAATGAGCTA CCGAATTTTA GACCCGGATA TCTTCTCAAT GTACAATTCT 
GGGACGTTGT TAGTGGATGA AAACTTAAAT TCAGATAATT ACCAAGCATT GCTTGATTTC 
ATGCTTTCAA GAAAAATGAG AAAGATCAAT CTCTCAGATG CCAATGAAAT TTCTTTTGGT 
GGTGTCAGAT TGAGTGAAGA TATCCCTAAA GGGGTAAACA CAAATGACCT GGAATCCGCT 
AGAGAGAAAA CAGCAAATCT TGGAATCATA GACCATTTTA TCAGGACCTT TGTTCACTGC 
AAAAGAGCAT TGGTTCTTGC TCATCGTGGT GGTTTCTGGA CCTTACTTCA GAATGTGACC 
CGGACTCTTT GGAATTACAC CATAGAGATG CAGTTACTCT ATAAACAGGC GCAGTTTCTT 
TCCAAAACAT TCCCTTTCAG CAAGGATATT ATGATATGCA CCTTCGTGAT GCCATTTACT 
TTGGGAGCAG ATGCCCTTAT TGACATGCTG TTAGACCTGC AGAATACCAA ATCTCTTAAG 
ATTGTGGATG ACTGTAAAGA TTTTGGTGTT GCAAGTGCTT ATGGGAACAT CACAGAGGAT 
AATGGTGGCT TCAATCTGAC ATTTGAAGAA CCTTTTGATG ATATGAATTT GGTTGACTTG 
AAATTCATTT CTGACTTCAT CTTTAAGACT TTGGAAATCT TGTACAGAGT GGGAAAATGG 
GAAACACTAG TCTACATTGC CCTTCAGTTC AATGAAGTGA CACATGAGAG ATACACTGAG 
CAAGTGATGC CATTGTTAAT CTATTCTCAA AGACAGCTTT TGCAGAGACT CCAGAGAAAT 
AGTGGGGAGC AGACAGATAA TATAGAATGG CAGTTTGATG ACACGGTAAA CCTCCGAGAT 
GTCATTGGGA AACTGCCCAA TTCTTCTTTT GTGCAGACTA TAAAGGGCAA GTCTAAAAGA 
CTTTTTAATT ATGAGCAAGG TGTAGATCTT TCGGAGACAT ACATTGCAAA TAGAATAGTG 
TCTGTGCCCC TGAATGTGAA AGAAACTTTG AAGACCTTTA GAGAAACCTT GACGAAATCT 
AAGTACCACA ACAGATCACT GAGATACAGC AGAAAATTGC TTTCTTTGTT ACTGGCCCAC 
ACACAAGAAG CCAGTGGAGG AATAAACAAT CCCAAATTTA TGTATGGAAA AGTGGAATTT 
AGCCTTGGAA CTGAAGAAAT ACATTCGCCT ACACCCCCTG ATCTTTTTAA TGAAAAGTTC 
ACTGCTTCTG TTACAGTACA GAGCACAAAA ATTTCTCCTT TCCAATTTCA ACTTGTTATC 
ACTTCTTATG AGAAAACGAT CGAGATTCTT GAAATCAGCA ATCAAACAGA TCTCAAGATA 
CAGGCACTAC ATGAGCTTGG AAATCTTCAC ATCTATGCAG AAAACAGAAG GGCAGCTTTT 
AAATGCTGGA GTCAAGCTCT TGATGCCATA CTCAAAAAAA AAGATGTTCT ACATACGTGG 
AAAGAAATTG TCAGCCCCAT CCAAAAACCT ATGAATAGCA TCGTGGGTTC AAACTACATC 
GATTATAGTG AAGATTTTCT GTTGAAAGCT GGCATTTGGG GATGCTTGCA GGGAGGAGTC 
ATAGCAGCAA AGATGGCCCA ATACATTTTA ACATCAAATA TGAATGAGAG GATTGAATGT 
TGCATTTTCT CTTCATTTTT TTTTAAGGGT CTACTTAGAA CCTCTCTTGT CCATCCCAAA 
TATGACCATG AGTATGCCCG TTATGAAATC ACCCAACTTA CTCCAGGGCT GGATCTCTTC 
TGTGACCGAT ACAGAGCAGA TGTGTCCATT GTCCTCAGCA GCCTGAGTTA CCTCTGTCAT 
GAACTCCATT CTGTCCACCA AGACCTTATT CTTCTGCCAC TTATTTCATT AAACCATTAC 
TTGGTCTGTG AAGTTTGCCA AGATCTATTT AAAACCATAG ATACAAGGAT CCTTAAGACG 
GAAATCCTTA TTGATTTAGG ATTCTTTTCT GAAGCTTTTA GTGAAATGGC TGGTCTTTAT 
ACTGGAAAAA ACATCCCCAC TTCCACGCCC TTAGGCTATA AACTTCCAAA AACTAAGATC 
ATGCAGCCCT TTCAAGCAGG ACACTCTTAT TTGAGCAAAG AAAATATACA GGCCCTGGAA 
GATTTAATGA ATCAAGGCAT CCCCCTATAC ATTATTTCGA TTTGCCAACC ATCTTTCTCC 
TATAAGTTTA CTTTGGTCAA AATGCGCTTT TTAATCAGTT TGGCTGCAAC CATAAATTGC 
TTCCCAGTTA GGGATACAAC AGCATTCTTC CAGGACTTTA CCCAGAAGAG CAAAGCCAGT 
GCTTCAGTCT TTAAAGGTAC AAACCCTGAA GATTCAATAA TCCCCTCTCA AGAGACAGTT 
CAAAATGACT TTACCGCAGC TGAAGGAACA CAGAGAGCTC TGGACAAGAG CATTGACTTC 
TCAAAGCAAA GAGGCTTCGA TGATATGAGG AAGATGCTGA TGGTGGACCC AGAATATTTA 
AAGGATGAAA CCCCAGCAAA TGTTAACCTG ACCAAGTCAA AAGAAGAGTT GTCTCCAGGT 
AAACTAAAGG GGATTTTATT GGCAGAAGCT GAGGACAAGC TTCTCATGCT AATAGAAAAT 
GCAGAGGAAT TGAGTCACAA GAAGATAACT CAGTTTAGCC CAATAGAATT GGAGATTATG 
ATAGAAGTCA AGTTACAGCT AGCCGCTATT GCGATGCAGA GACAACAAGC CCCACTGAGC 
ACTTCAATAG CTTTCACTGC AATTAAAATC CTTCAGGATG CAGACATTTT CAAGAAGACT 
AAAGAAGAAC TGTGTGCGAA TGATGATGAA CTGGATGCCA TGGAGTCTGT GGAGATGTTG 
GCCCGAGAAC GTCTAAACAT CCATCTATGG CTAAAGTGTC GTCTGGCCTT GCTGGTGGCC 
ACTGTGACCC ACATCCGTGG CATGGGGGTC GTGAAAGAAA ATGAGATGAT TGAGTGCAGA 
CTTTTGATAA ATGAAGTATT TGTGGAGGCT CAAAACTGGA ATGATACTGA GATGCAAGCA 
GAAGTCATGG TGCAGGCTGT CAAGTTTGAC CTGCAAGAAA GACATTCTGC AGCCAACATC 
TTAGAACATT TGAAGGATAT AAGAGGCTTA CTTGAAGGAA AATCCTTTGT CTCTCCTCGA 
GGCTCTTTAA TACTTATGAA AAGCCTGCTG TTAATGGATG ATTTAATGAA AATTGAGATG 
CCCCAGGAAT ATCTCTTCCT TAAAAATGAA AATCTCAATT TACTAAATCG AGCTCATGAG 
CTTATGATTG CGCAGGCTCT ATCTTTGGGT GAAAACATTT TATTGCCTTG TTCAGACACT 
GAATATGCAA ATATCTTCTA CCCTTTGAAA AACGTTTATT TTCCATACAT CAACTTACTT 
GCAAAAACAA AAATGAGGAT TGGGCATATG TTAGCCAAGA GCACTGCTGC CTGTGAGAGA 
AAAGAACCTC AGAAGTGGGA ACCCATTCGT CATCTCTTTG AGACAGCATT GCAACTTTGT 
AGGGCAGCTG CAGTGAAGGA ACGGGAGTTA GAAGCCGAAA TCCTTTTTGA GAAAGGCAAA 
GTAGAACGTC AAATGCTTGT TGTACAAGAC AACAAGCCTT TAATTGGTGA TCTTTTTGAT 
GCTATAAAAA TAAGTCTACA AAATGATGAA AATTCAGGGT TGATAAGGAA ATCCTATCTG 
GAAATCGCCC TGTTATATTT ACTTGTGTGG AATTATAGGA AAAATGCTTC AAAAAATAAG 
ATCTTTCTCC AAGTCCAGAA TCCATCAAAG GAATCACTAG AAATTAAAAC TGAAATTTAC 
CTGGCAATGT CCTGGATAGC AATCCGAGCT GCTGCACAGG TCAGTGAAGC TACAATGGCA 
GCTAAGAAGC TAACTGGAGA AAAGAGTGTG AAGACCAAGA AAATGGATCA AAATGTCTAT 
CAGAATATCC CTGAATTTGC TCATGCTGAC CTCACTTGTA CCTATGTAGA TTACTTGACT 
GCTAACTATA AAACTTGTGT CAAAACTTCC TATACCACTC CGAGCGAATC TGGAAGTTAT 
TTAAGTATGG AACAAGATCT GTACACCAAC CCCAGTGAAG AAAATGAGGA GCCCAGCCAG 
CTGGAGATCA CCTGGGTCCA TCTGGCCCGT TACTACACTC ACCTGTTAAG AGTTAACAAT 
ATGAATACCT TGCTTGCTCC TCCAAAGCCA GTTTTCTTAA CAGATGAAAT TTTCTACACT 
TCTGTTTTCA ATGAAGATCT GATTATACGT CAGAAAGAGA TGAAGAATTT CCTCAAAAAA 
TTCTTAAGTG TTTTTACTTC AGCCTGTCTA GAGTGTTTCC CCAAAGACCT CCTTCTTGGA 
ATAGAAAGAC TAAATATTCC AGATAATGCC GAACTTAGGC AATCACTTAA GACATTTCAA 
GATGTGAGTG CTATAATAGA AAAAATAGTC ACCACTAAGA TCAACCCCAT TATCCTTCCT 
CCATCTCCCC CGCATGAACA TATGGATTTT GCTATACATG CAGAAAGCCC AGATCTTTGC 
TTCCAGTGGT ATATACCCCC TCTGGAAAAA CCTCCAAGTG ATAAAGAACC CATGGTCATG 
TTGCTGTATG CCTATAACAC CAAACCTGTC AAGATTAATG ATATCTTTGA ATTCAATATC 
TCCTCGATAT TTCCTGGGTT TTTATGGCTT CCTCTGAACA AAGTTATAGC CTTACATGAA 
GCTCTATCTG GCCTTAGGCA AGAAACTGAA ATGTTGTTAT ACACAAAAAA ATTGCCTACA 
GAAGAAAAGA AGTCCCTACT ACCCTTCAGT GCCTTAAAAC CTGCTAGAGA AGTCTCTGAA 
GAGATCGTCC TCAGTAAGCA AATGAAGACT GTGTTTTATC AGAGTTGCAC TGAAATAGCA 
AGACTGCTTT CGAGAAATGA AGACATACCC CTAATAAGTG AGGTTCCATT TGAAGAATCT 
TTGCCAAGTA TACAGAAGTT GGAGATGCTT TTTGACCCCT CCAGTGGCTC TGTCTTAAAG 
GCAGAAGCCA TTTTCAACTG GATCGTGTCT TTTCTTACAT CTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CFAP54 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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