ESPL1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	100032809
Full Name	extra spindle pole bodies like 1, separase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007506532.2	XP_007506594.1	separin isoform X4
XM_007506528.2	XP_007506590.1	separin isoform X1
XM_007506529.2	XP_007506591.1	separin isoform X2
XM_007506530.2	XP_007506592.1	separin isoform X3
XM_007506531.2	XP_007506593.1	separin isoform X4

Pathways from BioSystems
Gene Ontology

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc36353	XM_007506528.2 Latest version!	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc36354	XM_007506529.2 Latest version!	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc36355	XM_007506530.2 Latest version!	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc36356	XM_007506531.2 Latest version!	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc36356	XM_007506532.2 Latest version!	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( XM_007506528.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007506590.1 Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc36353
Clone ID Related Accession (Same CDS sequence)	XM_007506528.2
Accession Version	XM_007506528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001584931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007506528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCAT CTAGTCGACC TTCTTATCCC CTCGCTTTTT TGTGGCTCTT AGTCCGTGAC 
CGAAGGCAAC TCATATGGAC CATGAGGAAC TTTAAAGGAG CAGACTTTGG AAAATGGATG 
AGCAGCCAAG AGGAGGCCAA GGCTTTGCTG GTTGAGCTGA AGGAGTTCCT GTCCAGACCT 
CTGGATAGCC CCACGGGCCG TTCATTTGCA GCAGAACAAA GACTTGCCTG TGATTCCATT 
TTGAGGGCTT GCAACCAACA GGTGGTGGCT GGGCTTGCTT GTCCTGACCA CTTGGGCAGC 
CTGTTGGAGC TGGCAGGGTT GGCCTGTGAT GGCTACTTGA AAGCAACCCC TCATCGCACC 
CCACTCTACT TAGAACGCAT CCTCTTCGTC TTCTTGCGAA ATATTTTTGC TCGGGTGGTC 
CCAAACGCTG TTCTCTACCT GATCAAATCA TTGCATACCT GCCTAGTGCA GCATGTGCCA 
GTGGGTGCAA CCCAGGACTA TGAGGCTATT ATCCGAGGTG CCTTCTCTTT GCTTTGGAAA 
GGGGCAGAAG CACTTAGAGA ACGTCAGCAA GCCTTCTCTG TTCGCCTGTG GGCCTTGAGT 
TTCTTGGTAC TCTTGGAAGA TGAAAGTACC CCATGCAAAT TCCCCCTTTT TTCTTCCCCA 
ACTGCTTGTC AAGCTGAAGC TGCCTGCCGG CTCTTTGAAG GCAGTGGGCA TCCTTTGGGT 
GAGGCAGATG CCCACTTCCT CAGTGAACAG CTTTCTACTT GTGTGATCAA AGTGCTGGTT 
GGGAAGGAAG CCAGTCCTTC ACATCCTCTC TCTCCCTGCA GGGCCCTCTG CCTCCTAGAG 
CTCACCTTAG AAAGGTGCCA AAGACTGTGC CTGAGCAGCT GCTGCACAGA GGCTGCTAGG 
GCTGTGCAGG AAGTCCAGGG TTATTTGGGG AACACAGCCC TGGCCCCTAG CCTCCAGCTG 
TGCCTGTTGG GGGTGGAACT CCGAAGGCTG CTGGCTGGAG CCTTTGGGGG AGTGGGGGAA 
CTATTGCGCA AGGCCGAAGC TATTCTCAGC TTTGGCGTCA AGGGCCCGTC ATCCTCATTT 
AGAGCTCTTG TGGAGACTTG TCAGTTCATC CTCTTGGTCC TAGAGAGTGA TACTTTGAAA 
AAGTATGAAT TGGATAGCAT CCTGGGCCTC TCAGCCTTCT TGGGCAAATA CTGTCATCTT 
CTTCGCCAGC TTCGGGACAG CGCTCCTGTG GATTGTCCCA AGCAGCAGCA AGTCTTGGCT 
CAGATGCATT TTCATGGACT CCAGCTCTTT ACCAGGATGG TTTATGATTT CTTCAGTGGT 
TCTAAGGTCA CAGATTTGAC TGACTTGACC GAGCTTGTTG AGAGCTGTCA TTGTGCTGTT 
GTCTGGATGT TGGAGGCTCT AGAGGGCCTG AAGGACCAAG AGCAGGCAGA CTACCTTGGA 
ATCACTGCCT GCTGCACCAG TCACTTGGCC TATAAGTTCT ACAACCAGAA GCTCTATGCT 
GAGGCCAGCA CCATCACTGA ACCTTTCTGT CAGAAGATTG GACTGGCAAC ATCTGGAACA 
TACCCTGGGG TGCCTCCTGA GAAGCTGCAC AAGTGCTTCC GGTTACATGT AGAGAGCCTG 
AAGAAGCTAG GCCAGCCAGC CCAGGGATGC AGGATGGTGG CTCTGTGGCT GAAGGCCCTG 
CAGCCCTGCA CCCTCGAGCA AATGGCGGAG CCCATCAGTT TCTGGGTCCG TGTCAAGATG 
GATGCCGCCA AGGCTGGTGA CAAGGAACTG CAGCTCAAGA CCCTTCGCGA TAGTCTGGCT 
GGTTGGGACA CAGAGCTGCT GACACCCTTG CTGGTAGAAG AGTTGCGAAG CTATAAAGTG 
ATCCGGGCCG ACACAGGACA AGAACGCTTC AATGTGATTT GTGACTTGTT GGAGCTGAGT 
ACAGAAGAGA CACCAGCTGG GGCCTGGGCA CGGGCCACCT ATTTGATAGA GCTTGCCCAA 
GTGCTGTGCT ACTATGACTT TGCCAAGCAT GCCAACTGCT CAGCTTTGGA GGCTGTGCAG 
GAGGCCCTGA AACTGTTAGA GTCTGTACAG CCGGAAGGCC AGGCCACAGA CCAGTTTTTG 
GATGATAAGG CACAGGCTTT GCTGTGGCTT TACATCTGTA GCCTGGAGAC CAAAATGCAG 
AAAGGCATTG AACAGGACTT GAGGCTGAAG TTGCAGCCCA GCTCGATAGA ATTGGAAACC 
AATGACTTGA ACTATGAGGA TAAAATCCAG GATGACCATT TCCTCTACAG TAGCATTGCC 
TTCAATCTGG CTGTGGATGC TGCTCAGTCT AAATGTTTAG ATCAGGCACT GGCCCTTTGG 
AAGGAGATGC TAAAAAAAGG GCAGGTGCCC ACCGTGAGAT GTCTACAACA GACCACTGCC 
TCCCTCCAGA TTCTGGCTGC TCTCTATCAG ATGGTGGCCA AGCCCCTACA GGCTCTGGAG 
ACACACTTGT TGCTCCGTAC TCTCTCAGAA AATGTTAAGG ACTACATTAG AGCAGCCAGT 
GCCTCCTGCC ATATCACTCG GCTCCTTCTG ACACTAGACT GTCCCGGCTA TGCTCAGTTC 
TACTTGGAGG AGGCAGAGTC CAGTCTGCAG CTCAGTGATC ACACCAGTGA TGGATACTTG 
CTCCACACCC AAACCTGTGC CCTACTTCGA AGTCAGCTTT ACTGCCTCCA GAAAAAGGTG 
ACTGAAGGCC TCTCTTTGTT GCTGTCTGTG GTACGGGATT CAACCCTTCA AAAATCTTCT 
AAGGCTTGGT ACTTGCTTCG GGTCCAGGCT TTGCAGGTGG TGGCTGTCTA CCTTAGTCTT 
CCCTCGAGCA GTCTTTCTGT CCAGCAGTGG GAGCAACTTT ATACTCAAGG CTGGCAAACC 
TCTGAGATAG CTCTCATTGA GTCCCACAAG CTCCTTCGAA GCATTATCAT CCTTCTGATG 
GGCAGAGACT TGCTCTTTGT TCAGCAGTCA GCAGTGGACG CTGCCTTTCT TGACTATGGT 
GAGAACCTAG TACAGAAGTG GCAAGTTCTT GCAGAGGTCC TGGGATGCTC AGAGAAGCTG 
GTAACCTTGT TGGGACGCCT GGGCACTGTA AGTGAAGCCA AGGCCTTCTG CCTGGAGGCA 
CTGAGGCTTG CCATGAAGCT GCAGTGTGTT CGCCAGTGTT CCCTGTTCCT GGTGCTAAAG 
GGAGAACTAG AACTAGCCCG AAATGATCTT GACCTTTGCC AGTCGGACTT AGAGCAAGTG 
CTGTTTCTGC TTGGGTCTAG TACAGAGTTC GAAGGGATAG CCCAGCTGCC AAACACTGTG 
AAAAGGATCC ATCCCCAGAA AGGACAACAG GAGCCTCGCA TACAGCCCGG TTCTGAGCTA 
TCTGAAGAGG AAGCCTTCCT GAAGGGTCCT GCTCTGCAGC TGGTAGCCAC TGTGGAGAAG 
GATCCTGGCC CCTCAGTGTC CTCCCCTGTT CTGAAAAGCA AGCCTCGGCC TTGCCCTGCC 
TTCCTTTCCC ACTCGCTTTC CTGCAGCTGC ATTCTTTGTA CTGACCCTGC TCTGGTAGTT 
GTCTGCCTTC GGTGGCTGCT AGTCTCTGCC GGGGCAGAAC TGTCCTCCGG TCATCAGGCC 
AAAGGCCTGG ACATGTTACG GTCTGTGCTC AGGAGATGCC CAGGGGCCAC ACATCATCTG 
GCACAGGTTT TACAAGCTAC TTTGAGCCAA GAAGCATCCC CCTCACCTGC TCCAGGATTC 
TTAGAGGACC TCATTGCTCA GGCTTATGCT CATTTAGCCC TAGAAGGCAT AAACCATCCA 
CCGGACAAAG GCCTCTGGGA CATCCTTGAG ACTGGCCTGA ATTTTGTTGC TTCCAGGACT 
CCTTTCCTGA ACCCCTGGCA TGCCAGCCTT CTTGTGACAA AGAGCCTGGC AACACTATCC 
AGTCTCAGCT GTTGTACCAC TCAGCTCTTT GGGAACACTT GGGCCTGGCA ACCTCCAGCA 
GCAAAGCTCT CCCAGATGTC AAAGTCTTCT AAAAATCAGA GCCAAAATGG TCCAGGAGAA 
AGAGGCAAAC AAACGGCCTC TAGTGACTTG GCCCTCCACA CTCCCTCCCC AGAGGAGCTG 
GGTGAGAAGC CTTCAGCCGG GCCAAAGCCC ATCAGGAAGC CTTGTACCCC GAAGCAGCCA 
GGTCAGGCTG GCCCCCATGC ATCCTTCACA GTGTTTGATG AGGAACTTTC TAGCCAAGGC 
AAATCCGATT TAGCCAAGGC CCCGAGGGTG AGGTGCAGAG TCCAAACTCG TCTGAAGGTG 
AACTTTAGCG ATGACGATGA AGATCGTCCG ACCCCTTCCC ATGGACTGGC TGAAAAACCC 
AAGAGACGAG GAGCTGCCCC TCGAGGCAGG GGGAGGGGGG GCAGGGCCTT GAGGCTGAAG 
ACGGATGCCC AAGGTGATCC AGGGGGCTCT GGGGACCAAT CTGCCTGTAT CAGGCGAACT 
CGCCAGAGCC ACAAAGCGGG GGTCGAGGAG CAAGTCCCTC AGAGGGCCCT TCGACGGCAG 
GCCAATGTAG ACGTGGAGCT CATGAGGACC ATTCCTGAAG AACAGACAAC CACGAAACTG 
GACATGAGTG TGGAGATGCT GCAAGATTCC GATGAGGGAG GCAGAGCCTC AGCAGGACAG 
AATGCCTGGC AGGACACTGC CATGGGAGAG TGTGAAGTGG TGAGACGAGA TGCCAGCAAG 
GAAGTGCCAC CTTTCCCACC CCTAGAGAAG AGGGAAGGTG ATCGAAGTCA GCATGGTCCC 
CAGACCAGGC CTGCATCTAC CCCGGCAGCC TTTGGCCAGC CCACCCTCGA ATCCATCTAT 
GACTCCCTGA GCATTGCCTT CCGCTGTGTC AGCCACTGCC CCCCGAGTGA GCTCTATGCT 
CAGCTGTGTC GTCTCCTTGC TCTGTGCCTG GGACACCGGG ACCCCCACAC CACTGCCTTG 
TTGGTCACTG AGTCTATGTC CATCACCTCT CGGCACCAGC TGCTCACCAC CGTGCACCGT 
CATCTCCACA AAGCCCAGAA GCGGCAAGCA GCAGATGTGG TGGAGCAGCT CCAGGGCCTG 
AGCCTACAGG AAGAGCCCAG AGGGGTCCCC TTTGGCCGCC TGCAGCACCT CTTTTCATTC 
AGGACACCCA CCAGTGGCTG CTTCCCGCAG CCCGAGAAGG AGAGCTTCCA GGAGCTTCTG 
GCTCAGATGC CTAGCGGAGT GACAGTGTGT GTGCTGGCTT TGGTCAGCCT CCAGTCTGGA 
ACTGTCAGGG ATACATTACT ATTGACACGG CTGGAAAAGA ACACTCCCCC CGTCACCATT 
CAGATACCCA CTGCCCAAAC AAAGCTGCCT CTGAGTTCGG CCTTGGAAGA GTTTAATTTA 
ATCCAGAAAG AACAGAAGGA GAATAGCAAC TGTACAGAGA AGTCCAAGTG GTGGGCAACT 
CGGCTGAAAC TGGATCGAAG GATGGAGGTA TTTGGTTGGG GCCCTTCTTT CCACAGGGAA 
TTTCCATCAT CTTCTCTTTA TGTGTCTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007506590.1
CDS	210..5702
Translation	MVASSRPSYPLAFLWLLVRDRRQLIWTMRNFKGADFGKWMSSQEEAKALLVELKEFLSRPLDSPTGRSFAAEQRLACDSILRACNQQVVAGLACPDHLGSLLELAGLACDGYLKATPHRTPLYLERILFVFLRNIFARVVPNAVLYLIKSLHTCLVQHVPVGATQDYEAIIRGAFSLLWKGAEALRERQQAFSVRLWALSFLVLLEDESTPCKFPLFSSPTACQAEAACRLFEGSGHPLGEADAHFLSEQLSTCVIKVLVGKEASPSHPLSPCRALCLLELTLERCQRLCLSSCCTEAARAVQEVQGYLGNTALAPSLQLCLLGVELRRLLAGAFGGVGELLRKAEAILSFGVKGPSSSFRALVETCQFILLVLESDTLKKYELDSILGLSAFLGKYCHLLRQLRDSAPVDCPKQQQVLAQMHFHGLQLFTRMVYDFFSGSKVTDLTDLTELVESCHCAVVWMLEALEGLKDQEQADYLGITACCTSHLAYKFYNQKLYAEASTITEPFCQKIGLATSGTYPGVPPEKLHKCFRLHVESLKKLGQPAQGCRMVALWLKALQPCTLEQMAEPISFWVRVKMDAAKAGDKELQLKTLRDSLAGWDTELLTPLLVEELRSYKVIRADTGQERFNVICDLLELSTEETPAGAWARATYLIELAQVLCYYDFAKHANCSALEAVQEALKLLESVQPEGQATDQFLDDKAQALLWLYICSLETKMQKGIEQDLRLKLQPSSIELETNDLNYEDKIQDDHFLYSSIAFNLAVDAAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASLQILAALYQMVAKPLQALETHLLLRTLSENVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGYLLHTQTCALLRSQLYCLQKKVTEGLSLLLSVVRDSTLQKSSKAWYLLRVQALQVVAVYLSLPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLRSIIILLMGRDLLFVQQSAVDAAFLDYGENLVQKWQVLAEVLGCSEKLVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQCSLFLVLKGELELARNDLDLCQSDLEQVLFLLGSSTEFEGIAQLPNTVKRIHPQKGQQEPRIQPGSELSEEEAFLKGPALQLVATVEKDPGPSVSSPVLKSKPRPCPAFLSHSLSCSCILCTDPALVVVCLRWLLVSAGAELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQATLSQEASPSPAPGFLEDLIAQAYAHLALEGINHPPDKGLWDILETGLNFVASRTPFLNPWHASLLVTKSLATLSSLSCCTTQLFGNTWAWQPPAAKLSQMSKSSKNQSQNGPGERGKQTASSDLALHTPSPEELGEKPSAGPKPIRKPCTPKQPGQAGPHASFTVFDEELSSQGKSDLAKAPRVRCRVQTRLKVNFSDDDEDRPTPSHGLAEKPKRRGAAPRGRGRGGRALRLKTDAQGDPGGSGDQSACIRRTRQSHKAGVEEQVPQRALRRQANVDVELMRTIPEEQTTTKLDMSVEMLQDSDEGGRASAGQNAWQDTAMGECEVVRRDASKEVPPFPPLEKREGDRSQHGPQTRPASTPAAFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLGHRDPHTTALLVTESMSITSRHQLLTTVHRHLHKAQKRQAADVVEQLQGLSLQEEPRGVPFGRLQHLFSFRTPTSGCFPQPEKESFQELLAQMPSGVTVCVLALVSLQSGTVRDTLLLTRLEKNTPPVTIQIPTAQTKLPLSSALEEFNLIQKEQKENSNCTEKSKWWATRLKLDRRMEVFGWGPSFHREFPSSSLYVSF

Target ORF information:

RefSeq Version	XM_007506528.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007506528.2

ORF Insert Sequence:

ATGGTGGCAT CTAGTCGACC TTCTTATCCC CTCGCTTTTT TGTGGCTCTT AGTCCGTGAC 
CGAAGGCAAC TCATATGGAC CATGAGGAAC TTTAAAGGAG CAGACTTTGG AAAATGGATG 
AGCAGCCAAG AGGAGGCCAA GGCTTTGCTG GTTGAGCTGA AGGAGTTCCT GTCCAGACCT 
CTGGATAGCC CCACGGGCCG TTCATTTGCA GCAGAACAAA GACTTGCCTG TGATTCCATT 
TTGAGGGCTT GCAACCAACA GGTGGTGGCT GGGCTTGCTT GTCCTGACCA CTTGGGCAGC 
CTGTTGGAGC TGGCAGGGTT GGCCTGTGAT GGCTACTTGA AAGCAACCCC TCATCGCACC 
CCACTCTACT TAGAACGCAT CCTCTTCGTC TTCTTGCGAA ATATTTTTGC TCGGGTGGTC 
CCAAACGCTG TTCTCTACCT GATCAAATCA TTGCATACCT GCCTAGTGCA GCATGTGCCA 
GTGGGTGCAA CCCAGGACTA TGAGGCTATT ATCCGAGGTG CCTTCTCTTT GCTTTGGAAA 
GGGGCAGAAG CACTTAGAGA ACGTCAGCAA GCCTTCTCTG TTCGCCTGTG GGCCTTGAGT 
TTCTTGGTAC TCTTGGAAGA TGAAAGTACC CCATGCAAAT TCCCCCTTTT TTCTTCCCCA 
ACTGCTTGTC AAGCTGAAGC TGCCTGCCGG CTCTTTGAAG GCAGTGGGCA TCCTTTGGGT 
GAGGCAGATG CCCACTTCCT CAGTGAACAG CTTTCTACTT GTGTGATCAA AGTGCTGGTT 
GGGAAGGAAG CCAGTCCTTC ACATCCTCTC TCTCCCTGCA GGGCCCTCTG CCTCCTAGAG 
CTCACCTTAG AAAGGTGCCA AAGACTGTGC CTGAGCAGCT GCTGCACAGA GGCTGCTAGG 
GCTGTGCAGG AAGTCCAGGG TTATTTGGGG AACACAGCCC TGGCCCCTAG CCTCCAGCTG 
TGCCTGTTGG GGGTGGAACT CCGAAGGCTG CTGGCTGGAG CCTTTGGGGG AGTGGGGGAA 
CTATTGCGCA AGGCCGAAGC TATTCTCAGC TTTGGCGTCA AGGGCCCGTC ATCCTCATTT 
AGAGCTCTTG TGGAGACTTG TCAGTTCATC CTCTTGGTCC TAGAGAGTGA TACTTTGAAA 
AAGTATGAAT TGGATAGCAT CCTGGGCCTC TCAGCCTTCT TGGGCAAATA CTGTCATCTT 
CTTCGCCAGC TTCGGGACAG CGCTCCTGTG GATTGTCCCA AGCAGCAGCA AGTCTTGGCT 
CAGATGCATT TTCATGGACT CCAGCTCTTT ACCAGGATGG TTTATGATTT CTTCAGTGGT 
TCTAAGGTCA CAGATTTGAC TGACTTGACC GAGCTTGTTG AGAGCTGTCA TTGTGCTGTT 
GTCTGGATGT TGGAGGCTCT AGAGGGCCTG AAGGACCAAG AGCAGGCAGA CTACCTTGGA 
ATCACTGCCT GCTGCACCAG TCACTTGGCC TATAAGTTCT ACAACCAGAA GCTCTATGCT 
GAGGCCAGCA CCATCACTGA ACCTTTCTGT CAGAAGATTG GACTGGCAAC ATCTGGAACA 
TACCCTGGGG TGCCTCCTGA GAAGCTGCAC AAGTGCTTCC GGTTACATGT AGAGAGCCTG 
AAGAAGCTAG GCCAGCCAGC CCAGGGATGC AGGATGGTGG CTCTGTGGCT GAAGGCCCTG 
CAGCCCTGCA CCCTCGAGCA AATGGCGGAG CCCATCAGTT TCTGGGTCCG TGTCAAGATG 
GATGCCGCCA AGGCTGGTGA CAAGGAACTG CAGCTCAAGA CCCTTCGCGA TAGTCTGGCT 
GGTTGGGACA CAGAGCTGCT GACACCCTTG CTGGTAGAAG AGTTGCGAAG CTATAAAGTG 
ATCCGGGCCG ACACAGGACA AGAACGCTTC AATGTGATTT GTGACTTGTT GGAGCTGAGT 
ACAGAAGAGA CACCAGCTGG GGCCTGGGCA CGGGCCACCT ATTTGATAGA GCTTGCCCAA 
GTGCTGTGCT ACTATGACTT TGCCAAGCAT GCCAACTGCT CAGCTTTGGA GGCTGTGCAG 
GAGGCCCTGA AACTGTTAGA GTCTGTACAG CCGGAAGGCC AGGCCACAGA CCAGTTTTTG 
GATGATAAGG CACAGGCTTT GCTGTGGCTT TACATCTGTA GCCTGGAGAC CAAAATGCAG 
AAAGGCATTG AACAGGACTT GAGGCTGAAG TTGCAGCCCA GCTCGATAGA ATTGGAAACC 
AATGACTTGA ACTATGAGGA TAAAATCCAG GATGACCATT TCCTCTACAG TAGCATTGCC 
TTCAATCTGG CTGTGGATGC TGCTCAGTCT AAATGTTTAG ATCAGGCACT GGCCCTTTGG 
AAGGAGATGC TAAAAAAAGG GCAGGTGCCC ACCGTGAGAT GTCTACAACA GACCACTGCC 
TCCCTCCAGA TTCTGGCTGC TCTCTATCAG ATGGTGGCCA AGCCCCTACA GGCTCTGGAG 
ACACACTTGT TGCTCCGTAC TCTCTCAGAA AATGTTAAGG ACTACATTAG AGCAGCCAGT 
GCCTCCTGCC ATATCACTCG GCTCCTTCTG ACACTAGACT GTCCCGGCTA TGCTCAGTTC 
TACTTGGAGG AGGCAGAGTC CAGTCTGCAG CTCAGTGATC ACACCAGTGA TGGATACTTG 
CTCCACACCC AAACCTGTGC CCTACTTCGA AGTCAGCTTT ACTGCCTCCA GAAAAAGGTG 
ACTGAAGGCC TCTCTTTGTT GCTGTCTGTG GTACGGGATT CAACCCTTCA AAAATCTTCT 
AAGGCTTGGT ACTTGCTTCG GGTCCAGGCT TTGCAGGTGG TGGCTGTCTA CCTTAGTCTT 
CCCTCGAGCA GTCTTTCTGT CCAGCAGTGG GAGCAACTTT ATACTCAAGG CTGGCAAACC 
TCTGAGATAG CTCTCATTGA GTCCCACAAG CTCCTTCGAA GCATTATCAT CCTTCTGATG 
GGCAGAGACT TGCTCTTTGT TCAGCAGTCA GCAGTGGACG CTGCCTTTCT TGACTATGGT 
GAGAACCTAG TACAGAAGTG GCAAGTTCTT GCAGAGGTCC TGGGATGCTC AGAGAAGCTG 
GTAACCTTGT TGGGACGCCT GGGCACTGTA AGTGAAGCCA AGGCCTTCTG CCTGGAGGCA 
CTGAGGCTTG CCATGAAGCT GCAGTGTGTT CGCCAGTGTT CCCTGTTCCT GGTGCTAAAG 
GGAGAACTAG AACTAGCCCG AAATGATCTT GACCTTTGCC AGTCGGACTT AGAGCAAGTG 
CTGTTTCTGC TTGGGTCTAG TACAGAGTTC GAAGGGATAG CCCAGCTGCC AAACACTGTG 
AAAAGGATCC ATCCCCAGAA AGGACAACAG GAGCCTCGCA TACAGCCCGG TTCTGAGCTA 
TCTGAAGAGG AAGCCTTCCT GAAGGGTCCT GCTCTGCAGC TGGTAGCCAC TGTGGAGAAG 
GATCCTGGCC CCTCAGTGTC CTCCCCTGTT CTGAAAAGCA AGCCTCGGCC TTGCCCTGCC 
TTCCTTTCCC ACTCGCTTTC CTGCAGCTGC ATTCTTTGTA CTGACCCTGC TCTGGTAGTT 
GTCTGCCTTC GGTGGCTGCT AGTCTCTGCC GGGGCAGAAC TGTCCTCCGG TCATCAGGCC 
AAAGGCCTGG ACATGTTACG GTCTGTGCTC AGGAGATGCC CAGGGGCCAC ACATCATCTG 
GCACAGGTTT TACAAGCTAC TTTGAGCCAA GAAGCATCCC CCTCACCTGC TCCAGGATTC 
TTAGAGGACC TCATTGCTCA GGCTTATGCT CATTTAGCCC TAGAAGGCAT AAACCATCCA 
CCGGACAAAG GCCTCTGGGA CATCCTTGAG ACTGGCCTGA ATTTTGTTGC TTCCAGGACT 
CCTTTCCTGA ACCCCTGGCA TGCCAGCCTT CTTGTGACAA AGAGCCTGGC AACACTATCC 
AGTCTCAGCT GTTGTACCAC TCAGCTCTTT GGGAACACTT GGGCCTGGCA ACCTCCAGCA 
GCAAAGCTCT CCCAGATGTC AAAGTCTTCT AAAAATCAGA GCCAAAATGG TCCAGGAGAA 
AGAGGCAAAC AAACGGCCTC TAGTGACTTG GCCCTCCACA CTCCCTCCCC AGAGGAGCTG 
GGTGAGAAGC CTTCAGCCGG GCCAAAGCCC ATCAGGAAGC CTTGTACCCC GAAGCAGCCA 
GGTCAGGCTG GCCCCCATGC ATCCTTCACA GTGTTTGATG AGGAACTTTC TAGCCAAGGC 
AAATCCGATT TAGCCAAGGC CCCGAGGGTG AGGTGCAGAG TCCAAACTCG TCTGAAGGTG 
AACTTTAGCG ATGACGATGA AGATCGTCCG ACCCCTTCCC ATGGACTGGC TGAAAAACCC 
AAGAGACGAG GAGCTGCCCC TCGAGGCAGG GGGAGGGGGG GCAGGGCCTT GAGGCTGAAG 
ACGGATGCCC AAGGTGATCC AGGGGGCTCT GGGGACCAAT CTGCCTGTAT CAGGCGAACT 
CGCCAGAGCC ACAAAGCGGG GGTCGAGGAG CAAGTCCCTC AGAGGGCCCT TCGACGGCAG 
GCCAATGTAG ACGTGGAGCT CATGAGGACC ATTCCTGAAG AACAGACAAC CACGAAACTG 
GACATGAGTG TGGAGATGCT GCAAGATTCC GATGAGGGAG GCAGAGCCTC AGCAGGACAG 
AATGCCTGGC AGGACACTGC CATGGGAGAG TGTGAAGTGG TGAGACGAGA TGCCAGCAAG 
GAAGTGCCAC CTTTCCCACC CCTAGAGAAG AGGGAAGGTG ATCGAAGTCA GCATGGTCCC 
CAGACCAGGC CTGCATCTAC CCCGGCAGCC TTTGGCCAGC CCACCCTCGA ATCCATCTAT 
GACTCCCTGA GCATTGCCTT CCGCTGTGTC AGCCACTGCC CCCCGAGTGA GCTCTATGCT 
CAGCTGTGTC GTCTCCTTGC TCTGTGCCTG GGACACCGGG ACCCCCACAC CACTGCCTTG 
TTGGTCACTG AGTCTATGTC CATCACCTCT CGGCACCAGC TGCTCACCAC CGTGCACCGT 
CATCTCCACA AAGCCCAGAA GCGGCAAGCA GCAGATGTGG TGGAGCAGCT CCAGGGCCTG 
AGCCTACAGG AAGAGCCCAG AGGGGTCCCC TTTGGCCGCC TGCAGCACCT CTTTTCATTC 
AGGACACCCA CCAGTGGCTG CTTCCCGCAG CCCGAGAAGG AGAGCTTCCA GGAGCTTCTG 
GCTCAGATGC CTAGCGGAGT GACAGTGTGT GTGCTGGCTT TGGTCAGCCT CCAGTCTGGA 
ACTGTCAGGG ATACATTACT ATTGACACGG CTGGAAAAGA ACACTCCCCC CGTCACCATT 
CAGATACCCA CTGCCCAAAC AAAGCTGCCT CTGAGTTCGG CCTTGGAAGA GTTTAATTTA 
ATCCAGAAAG AACAGAAGGA GAATAGCAAC TGTACAGAGA AGTCCAAGTG GTGGGCAACT 
CGGCTGAAAC TGGATCGAAG GATGGAGGTA TTTGGTTGGG GCCCTTCTTT CCACAGGGAA 
TTTCCATCAT CTTCTCTTTA TGTGTCTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_007506529.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007506591.1 Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OMc36354
Clone ID Related Accession (Same CDS sequence)	XM_007506529.2
Accession Version	XM_007506529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001584931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007506529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCAT CTAGTCGACC TTCTTATCCC CTCGCTTTTT TGTGGCTCTT AGTCCGTGAC 
CGAAGGCAAC TCATATGGAC CATGAGGAAC TTTAAAGGAG CAGACTTTGG AAAATGGATG 
AGCAGCCAAG AGGAGGCCAA GGCTTTGCTG GTTGAGCTGA AGGAGTTCCT GTCCAGACCT 
CTGGATAGCC CCACGGGCCG TTCATTTGCA GCAGAACAAA GACTTGCCTG TGATTCCATT 
TTGAGGGCTT GCAACCAACA GGTGGTGGCT GGGCTTGCTT GTCCTGACCA CTTGGGCAGC 
CTGTTGGAGC TGGCAGGGTT GGCCTGTGAT GGCTACTTGA AAGCAACCCC TCATCGCACC 
CCACTCTACT TAGAACGCAT CCTCTTCGTC TTCTTGCGAA ATATTTTTGC TCGGGTGGTC 
CCAAACGCTG TTCTCTACCT GATCAAATCA TTGCATACCT GCCTAGTGCA GCATGTGCCA 
GTGGGTGCAA CCCAGGACTA TGAGGCTATT ATCCGAGGTG CCTTCTCTTT GCTTTGGAAA 
GGGGCAGAAG CACTTAGAGA ACGTCAGCAA GCCTTCTCTG TTCGCCTGTG GGCCTTGAGT 
TTCTTGGTAC TCTTGGAAGA TGAAAGTACC CCATGCAAAT TCCCCCTTTT TTCTTCCCCA 
ACTGCTTGTC AAGCTGAAGC TGCCTGCCGG CTCTTTGAAG GCAGTGGGCA TCCTTTGGGT 
GAGGCAGATG CCCACTTCCT CAGTGAACAG CTTTCTACTT GTGTGATCAA AGTGCTGGTT 
GGGAAGGAAG CCAGTCCTTC ACATCCTCTC TCTCCCTGCA GGGCCCTCTG CCTCCTAGAG 
CTCACCTTAG AAAGGTGCCA AAGACTGTGC CTGAGCAGCT GCTGCACAGA GGCTGCTAGG 
GCTGTGCAGG AAGTCCAGGG TTATTTGGGG AACACAGCCC TGGCCCCTAG CCTCCAGCTG 
TGCCTGTTGG GGGTGGAACT CCGAAGGCTG CTGGCTGGAG CCTTTGGGGG AGTGGGGGAA 
CTATTGCGCA AGGCCGAAGC TATTCTCAGC TTTGGCGTCA AGGGCCCGTC ATCCTCATTT 
AGAGCTCTTG TGGAGACTTG TCAGTTCATC CTCTTGGTCC TAGAGAGTGA TACTTTGAAA 
AAGTATGAAT TGGATAGCAT CCTGGGCCTC TCAGCCTTCT TGGGCAAATA CTGTCATCTT 
CTTCGCCAGC TTCGGGACAG CGCTCCTGTG GATTGTCCCA AGCAGCAGCA AGTCTTGGCT 
CAGATGCATT TTCATGGACT CCAGCTCTTT ACCAGGATGG TTTATGATTT CTTCAGTGGT 
TCTAAGGTCA CAGATTTGAC TGACTTGACC GAGCTTGTTG AGAGCTGTCA TTGTGCTGTT 
GTCTGGATGT TGGAGGCTCT AGAGGGCCTG AAGGACCAAG AGCAGGCAGA CTACCTTGGA 
ATCACTGCCT GCTGCACCAG TCACTTGGCC TATAAGTTCT ACAACCAGAA GCTCTATGCT 
GAGGCCAGCA CCATCACTGA ACCTTTCTGT CAGAAGATTG GACTGGCAAC ATCTGGAACA 
TACCCTGGGG TGCCTCCTGA GAAGCTGCAC AAGTGCTTCC GGTTACATGT AGAGAGCCTG 
AAGAAGCTAG GCCAGCCAGC CCAGGGATGC AGGATGGTGG CTCTGTGGCT GAAGGCCCTG 
CAGCCCTGCA CCCTCGAGCA AATGGCGGAG CCCATCAGTT TCTGGGTCCG TGTCAAGATG 
GATGCCGCCA AGGCTGGTGA CAAGGAACTG CAGCTCAAGA CCCTTCGCGA TAGTCTGGCT 
GGTTGGGACA CAGAGCTGCT GACACCCTTG CTGGTAGAAG AGTTGCGAAG CTATAAAGTG 
ATCCGGGCCG ACACAGGACA AGAACGCTTC AATGTGATTT GTGACTTGTT GGAGCTGAGT 
ACAGAAGAGA CACCAGCTGG GGCCTGGGCA CGGGCCACCT ATTTGATAGA GCTTGCCCAA 
GTGCTGTGCT ACTATGACTT TGCCAAGCAT GCCAACTGCT CAGCTTTGGA GGCTGTGCAG 
GAGGCCCTGA AACTGTTAGA GTCTGTACAG CCGGAAGGCC AGGCCACAGA CCAGTTTTTG 
GATGATAAGG CACAGGCTTT GCTGTGGCTT TACATCTGTA GCCTGGAGAC CAAAATGCAG 
AAAGGCATTG AACAGGACTT GAGGCTGAAG TTGCAGCCCA GCTCGATAGA ATTGGAAACC 
AATGACTTGA ACTATGAGGA TAAAATCCAG GATGACCATT TCCTCTACAG TAGCATTGCC 
TTCAATCTGG CTGTGGATGC TGCTCAGTCT AAATGTTTAG ATCAGGCACT GGCCCTTTGG 
AAGGAGATGC TAAAAAAAGG GCAGGTGCCC ACCGTGAGAT GTCTACAACA GACCACTGCC 
TCCCTCCAGA TTCTGGCTGC TCTCTATCAG ATGGTGGCCA AGCCCCTACA GGCTCTGGAG 
ACACACTTGT TGCTCCGTAC TCTCTCAGAA AATGTTAAGG ACTACATTAG AGCAGCCAGT 
GCCTCCTGCC ATATCACTCG GCTCCTTCTG ACACTAGACT GTCCCGGCTA TGCTCAGTTC 
TACTTGGAGG AGGCAGAGTC CAGTCTGCAG CTCAGTGATC ACACCAGTGA TGGATACTTG 
CTCCACACCC AAACCTGTGC CCTACTTCGA AGTCAGCTTT ACTGCCTCCA GAAAAAGGTG 
ACTGAAGGCC TCTCTTTGTT GCTGTCTGTG GTACGGGATT CAACCCTTCA AAAATCTTCT 
AAGGCTTGGT ACTTGCTTCG GGTCCAGGCT TTGCAGGTGG TGGCTGTCTA CCTTAGTCTT 
CCCTCGAGCA GTCTTTCTGT CCAGCAGTGG GAGCAACTTT ATACTCAAGG CTGGCAAACC 
TCTGAGATAG CTCTCATTGA GTCCCACAAG CTCCTTCGAA GCATTATCAT CCTTCTGATG 
GGCAGAGACT TGCTCTTTGT TCAGCAGTCA GCAGTGGACG CTGCCTTTCT TGACTATGGT 
GAGAACCTAG TACAGAAGTG GCAAGTTCTT GCAGAGGTCC TGGGATGCTC AGAGAAGCTG 
GTAACCTTGT TGGGACGCCT GGGCACTGTA AGTGAAGCCA AGGCCTTCTG CCTGGAGGCA 
CTGAGGCTTG CCATGAAGCT GCAGTGTGTT CGCCAGTGTT CCCTGTTCCT GGTGCTAAAG 
GGAGAACTAG AACTAGCCCG AAATGATCTT GACCTTTGCC AGTCGGACTT AGAGCAAGTG 
CTGTTTCTGC TTGGGTCTAG TACAGAGTTC GAAGGGATAG CCCAGCTGCC AAACACTGTG 
AAAAGGATCC ATCCCCAGAA AGGACAACAG GAGCCTCGCA TACAGCCCGG TTCTGAGCTA 
TCTGAAGAGG AAGCCTTCCT GAAGGGTCCT GCTCTGCAGC TGGTAGCCAC TGTGGAGAAG 
GATCCTGGCC CCTCAGTGTC CTCCCCTGTT CTGAAAAGCA AGCCTCGGCC TTGCCCTGCC 
TTCCTTTCCC ACTCGCTTTC CTGCAGCTGC ATTCTTTGTA CTGACCCTGC TCTGGTAGTT 
GTCTGCCTTC GGTGGCTGCT AGTCTCTGCC GGGGCAGAAC TGTCCTCCGG TCATCAGGCC 
AAAGGCCTGG ACATGTTACG GTCTGTGCTC AGGAGATGCC CAGGGGCCAC ACATCATCTG 
GCACAGGTTT TACAAGCTAC TTTGAGCCAA GAAGCATCCC CCTCACCTGC TCCAGGATTC 
TTAGAGGACC TCATTGCTCA GGCTTATGCT CATTTAGCCC TAGAAGGCAT AAACCATCCA 
CCGGACAAAG GCCTCTGGGA CATCCTTGAG ACTGGCCTGA ATTTTGTTGC TTCCAGGACT 
CCTTTCCTGA ACCCCTGGCA TGCCAGCCTT CTTGTGACAA AGAGCCTGGC AACACTATCC 
AGTCTCAGCT GTTGTACCAC TCAGCTCTTT GGGAACACTT GGGCCTGGCA ACCTCCAGCA 
GCAAAGCTCT CCCAGATGTC AAAGTCTTCT AAAAATCAGA GCCAAAATGG TCCAGGAGAA 
AGAGGCAAAC AAACGGCCTC TAGTGACTTG GCCCTCCACA CTCCCTCCCC AGAGGAGCTG 
GGTGAGAAGC CTTCAGCCGG GCCAAAGCCC ATCAGGAAGC CTTGTACCCC GAAGCAGCCA 
GGTCAGGCTG GCCCCCATGC ATCCTTCACA GTGTTTGATG AGGAACTTTC TAGCCAAGGC 
AAATCCGATT TAGCCAAGGC CCCGAGGGTG AGGTGCAGAG TCCAAACTCG TCTGAAGGTG 
AACTTTAGCG ATGACGATGA AGATCGTCCG ACCCCTTCCC ATGGACTGGC TGAAAAACCC 
AAGAGACGAG GAGCTGCCCC TCGAGGCAGG GGGAGGGGGG GCAGGGCCTT GAGGCTGAAG 
ACGGATGCCC AAGGTGATCC AGGGGGCTCT GGGGACCAAT CTGCCTGTAT CAGGCGAACT 
CGCCAGAGCC ACAAAGCGGG GGTCGAGGAG CAAGTCCCTC AGAGGGCCCT TCGACGGCAG 
GCCAATGTAG ACGTGGAGCT CATGAGGACC ATTCCTGAAG AACAGACAAC CACGAAACTG 
GACATGAGTG TGGAGATGCT GCAAGATTCC GATGAGGGAG GCAGAGCCTC AGGACAGAAT 
GCCTGGCAGG ACACTGCCAT GGGAGAGTGT GAAGTGGTGA GACGAGATGC CAGCAAGGAA 
GTGCCACCTT TCCCACCCCT AGAGAAGAGG GAAGGTGATC GAAGTCAGCA TGGTCCCCAG 
ACCAGGCCTG CATCTACCCC GGCAGCCTTT GGCCAGCCCA CCCTCGAATC CATCTATGAC 
TCCCTGAGCA TTGCCTTCCG CTGTGTCAGC CACTGCCCCC CGAGTGAGCT CTATGCTCAG 
CTGTGTCGTC TCCTTGCTCT GTGCCTGGGA CACCGGGACC CCCACACCAC TGCCTTGTTG 
GTCACTGAGT CTATGTCCAT CACCTCTCGG CACCAGCTGC TCACCACCGT GCACCGTCAT 
CTCCACAAAG CCCAGAAGCG GCAAGCAGCA GATGTGGTGG AGCAGCTCCA GGGCCTGAGC 
CTACAGGAAG AGCCCAGAGG GGTCCCCTTT GGCCGCCTGC AGCACCTCTT TTCATTCAGG 
ACACCCACCA GTGGCTGCTT CCCGCAGCCC GAGAAGGAGA GCTTCCAGGA GCTTCTGGCT 
CAGATGCCTA GCGGAGTGAC AGTGTGTGTG CTGGCTTTGG TCAGCCTCCA GTCTGGAACT 
GTCAGGGATA CATTACTATT GACACGGCTG GAAAAGAACA CTCCCCCCGT CACCATTCAG 
ATACCCACTG CCCAAACAAA GCTGCCTCTG AGTTCGGCCT TGGAAGAGTT TAATTTAATC 
CAGAAAGAAC AGAAGGAGAA TAGCAACTGT ACAGAGAAGT CCAAGTGGTG GGCAACTCGG 
CTGAAACTGG ATCGAAGGAT GGAGGTATTT GGTTGGGGCC CTTCTTTCCA CAGGGAATTT 
CCATCATCTT CTCTTTATGT GTCTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007506591.1
CDS	210..5699
Translation	MVASSRPSYPLAFLWLLVRDRRQLIWTMRNFKGADFGKWMSSQEEAKALLVELKEFLSRPLDSPTGRSFAAEQRLACDSILRACNQQVVAGLACPDHLGSLLELAGLACDGYLKATPHRTPLYLERILFVFLRNIFARVVPNAVLYLIKSLHTCLVQHVPVGATQDYEAIIRGAFSLLWKGAEALRERQQAFSVRLWALSFLVLLEDESTPCKFPLFSSPTACQAEAACRLFEGSGHPLGEADAHFLSEQLSTCVIKVLVGKEASPSHPLSPCRALCLLELTLERCQRLCLSSCCTEAARAVQEVQGYLGNTALAPSLQLCLLGVELRRLLAGAFGGVGELLRKAEAILSFGVKGPSSSFRALVETCQFILLVLESDTLKKYELDSILGLSAFLGKYCHLLRQLRDSAPVDCPKQQQVLAQMHFHGLQLFTRMVYDFFSGSKVTDLTDLTELVESCHCAVVWMLEALEGLKDQEQADYLGITACCTSHLAYKFYNQKLYAEASTITEPFCQKIGLATSGTYPGVPPEKLHKCFRLHVESLKKLGQPAQGCRMVALWLKALQPCTLEQMAEPISFWVRVKMDAAKAGDKELQLKTLRDSLAGWDTELLTPLLVEELRSYKVIRADTGQERFNVICDLLELSTEETPAGAWARATYLIELAQVLCYYDFAKHANCSALEAVQEALKLLESVQPEGQATDQFLDDKAQALLWLYICSLETKMQKGIEQDLRLKLQPSSIELETNDLNYEDKIQDDHFLYSSIAFNLAVDAAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASLQILAALYQMVAKPLQALETHLLLRTLSENVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGYLLHTQTCALLRSQLYCLQKKVTEGLSLLLSVVRDSTLQKSSKAWYLLRVQALQVVAVYLSLPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLRSIIILLMGRDLLFVQQSAVDAAFLDYGENLVQKWQVLAEVLGCSEKLVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQCSLFLVLKGELELARNDLDLCQSDLEQVLFLLGSSTEFEGIAQLPNTVKRIHPQKGQQEPRIQPGSELSEEEAFLKGPALQLVATVEKDPGPSVSSPVLKSKPRPCPAFLSHSLSCSCILCTDPALVVVCLRWLLVSAGAELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQATLSQEASPSPAPGFLEDLIAQAYAHLALEGINHPPDKGLWDILETGLNFVASRTPFLNPWHASLLVTKSLATLSSLSCCTTQLFGNTWAWQPPAAKLSQMSKSSKNQSQNGPGERGKQTASSDLALHTPSPEELGEKPSAGPKPIRKPCTPKQPGQAGPHASFTVFDEELSSQGKSDLAKAPRVRCRVQTRLKVNFSDDDEDRPTPSHGLAEKPKRRGAAPRGRGRGGRALRLKTDAQGDPGGSGDQSACIRRTRQSHKAGVEEQVPQRALRRQANVDVELMRTIPEEQTTTKLDMSVEMLQDSDEGGRASGQNAWQDTAMGECEVVRRDASKEVPPFPPLEKREGDRSQHGPQTRPASTPAAFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLGHRDPHTTALLVTESMSITSRHQLLTTVHRHLHKAQKRQAADVVEQLQGLSLQEEPRGVPFGRLQHLFSFRTPTSGCFPQPEKESFQELLAQMPSGVTVCVLALVSLQSGTVRDTLLLTRLEKNTPPVTIQIPTAQTKLPLSSALEEFNLIQKEQKENSNCTEKSKWWATRLKLDRRMEVFGWGPSFHREFPSSSLYVSF

Target ORF information:

RefSeq Version	XM_007506529.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007506529.2

ORF Insert Sequence:

ATGGTGGCAT CTAGTCGACC TTCTTATCCC CTCGCTTTTT TGTGGCTCTT AGTCCGTGAC 
CGAAGGCAAC TCATATGGAC CATGAGGAAC TTTAAAGGAG CAGACTTTGG AAAATGGATG 
AGCAGCCAAG AGGAGGCCAA GGCTTTGCTG GTTGAGCTGA AGGAGTTCCT GTCCAGACCT 
CTGGATAGCC CCACGGGCCG TTCATTTGCA GCAGAACAAA GACTTGCCTG TGATTCCATT 
TTGAGGGCTT GCAACCAACA GGTGGTGGCT GGGCTTGCTT GTCCTGACCA CTTGGGCAGC 
CTGTTGGAGC TGGCAGGGTT GGCCTGTGAT GGCTACTTGA AAGCAACCCC TCATCGCACC 
CCACTCTACT TAGAACGCAT CCTCTTCGTC TTCTTGCGAA ATATTTTTGC TCGGGTGGTC 
CCAAACGCTG TTCTCTACCT GATCAAATCA TTGCATACCT GCCTAGTGCA GCATGTGCCA 
GTGGGTGCAA CCCAGGACTA TGAGGCTATT ATCCGAGGTG CCTTCTCTTT GCTTTGGAAA 
GGGGCAGAAG CACTTAGAGA ACGTCAGCAA GCCTTCTCTG TTCGCCTGTG GGCCTTGAGT 
TTCTTGGTAC TCTTGGAAGA TGAAAGTACC CCATGCAAAT TCCCCCTTTT TTCTTCCCCA 
ACTGCTTGTC AAGCTGAAGC TGCCTGCCGG CTCTTTGAAG GCAGTGGGCA TCCTTTGGGT 
GAGGCAGATG CCCACTTCCT CAGTGAACAG CTTTCTACTT GTGTGATCAA AGTGCTGGTT 
GGGAAGGAAG CCAGTCCTTC ACATCCTCTC TCTCCCTGCA GGGCCCTCTG CCTCCTAGAG 
CTCACCTTAG AAAGGTGCCA AAGACTGTGC CTGAGCAGCT GCTGCACAGA GGCTGCTAGG 
GCTGTGCAGG AAGTCCAGGG TTATTTGGGG AACACAGCCC TGGCCCCTAG CCTCCAGCTG 
TGCCTGTTGG GGGTGGAACT CCGAAGGCTG CTGGCTGGAG CCTTTGGGGG AGTGGGGGAA 
CTATTGCGCA AGGCCGAAGC TATTCTCAGC TTTGGCGTCA AGGGCCCGTC ATCCTCATTT 
AGAGCTCTTG TGGAGACTTG TCAGTTCATC CTCTTGGTCC TAGAGAGTGA TACTTTGAAA 
AAGTATGAAT TGGATAGCAT CCTGGGCCTC TCAGCCTTCT TGGGCAAATA CTGTCATCTT 
CTTCGCCAGC TTCGGGACAG CGCTCCTGTG GATTGTCCCA AGCAGCAGCA AGTCTTGGCT 
CAGATGCATT TTCATGGACT CCAGCTCTTT ACCAGGATGG TTTATGATTT CTTCAGTGGT 
TCTAAGGTCA CAGATTTGAC TGACTTGACC GAGCTTGTTG AGAGCTGTCA TTGTGCTGTT 
GTCTGGATGT TGGAGGCTCT AGAGGGCCTG AAGGACCAAG AGCAGGCAGA CTACCTTGGA 
ATCACTGCCT GCTGCACCAG TCACTTGGCC TATAAGTTCT ACAACCAGAA GCTCTATGCT 
GAGGCCAGCA CCATCACTGA ACCTTTCTGT CAGAAGATTG GACTGGCAAC ATCTGGAACA 
TACCCTGGGG TGCCTCCTGA GAAGCTGCAC AAGTGCTTCC GGTTACATGT AGAGAGCCTG 
AAGAAGCTAG GCCAGCCAGC CCAGGGATGC AGGATGGTGG CTCTGTGGCT GAAGGCCCTG 
CAGCCCTGCA CCCTCGAGCA AATGGCGGAG CCCATCAGTT TCTGGGTCCG TGTCAAGATG 
GATGCCGCCA AGGCTGGTGA CAAGGAACTG CAGCTCAAGA CCCTTCGCGA TAGTCTGGCT 
GGTTGGGACA CAGAGCTGCT GACACCCTTG CTGGTAGAAG AGTTGCGAAG CTATAAAGTG 
ATCCGGGCCG ACACAGGACA AGAACGCTTC AATGTGATTT GTGACTTGTT GGAGCTGAGT 
ACAGAAGAGA CACCAGCTGG GGCCTGGGCA CGGGCCACCT ATTTGATAGA GCTTGCCCAA 
GTGCTGTGCT ACTATGACTT TGCCAAGCAT GCCAACTGCT CAGCTTTGGA GGCTGTGCAG 
GAGGCCCTGA AACTGTTAGA GTCTGTACAG CCGGAAGGCC AGGCCACAGA CCAGTTTTTG 
GATGATAAGG CACAGGCTTT GCTGTGGCTT TACATCTGTA GCCTGGAGAC CAAAATGCAG 
AAAGGCATTG AACAGGACTT GAGGCTGAAG TTGCAGCCCA GCTCGATAGA ATTGGAAACC 
AATGACTTGA ACTATGAGGA TAAAATCCAG GATGACCATT TCCTCTACAG TAGCATTGCC 
TTCAATCTGG CTGTGGATGC TGCTCAGTCT AAATGTTTAG ATCAGGCACT GGCCCTTTGG 
AAGGAGATGC TAAAAAAAGG GCAGGTGCCC ACCGTGAGAT GTCTACAACA GACCACTGCC 
TCCCTCCAGA TTCTGGCTGC TCTCTATCAG ATGGTGGCCA AGCCCCTACA GGCTCTGGAG 
ACACACTTGT TGCTCCGTAC TCTCTCAGAA AATGTTAAGG ACTACATTAG AGCAGCCAGT 
GCCTCCTGCC ATATCACTCG GCTCCTTCTG ACACTAGACT GTCCCGGCTA TGCTCAGTTC 
TACTTGGAGG AGGCAGAGTC CAGTCTGCAG CTCAGTGATC ACACCAGTGA TGGATACTTG 
CTCCACACCC AAACCTGTGC CCTACTTCGA AGTCAGCTTT ACTGCCTCCA GAAAAAGGTG 
ACTGAAGGCC TCTCTTTGTT GCTGTCTGTG GTACGGGATT CAACCCTTCA AAAATCTTCT 
AAGGCTTGGT ACTTGCTTCG GGTCCAGGCT TTGCAGGTGG TGGCTGTCTA CCTTAGTCTT 
CCCTCGAGCA GTCTTTCTGT CCAGCAGTGG GAGCAACTTT ATACTCAAGG CTGGCAAACC 
TCTGAGATAG CTCTCATTGA GTCCCACAAG CTCCTTCGAA GCATTATCAT CCTTCTGATG 
GGCAGAGACT TGCTCTTTGT TCAGCAGTCA GCAGTGGACG CTGCCTTTCT TGACTATGGT 
GAGAACCTAG TACAGAAGTG GCAAGTTCTT GCAGAGGTCC TGGGATGCTC AGAGAAGCTG 
GTAACCTTGT TGGGACGCCT GGGCACTGTA AGTGAAGCCA AGGCCTTCTG CCTGGAGGCA 
CTGAGGCTTG CCATGAAGCT GCAGTGTGTT CGCCAGTGTT CCCTGTTCCT GGTGCTAAAG 
GGAGAACTAG AACTAGCCCG AAATGATCTT GACCTTTGCC AGTCGGACTT AGAGCAAGTG 
CTGTTTCTGC TTGGGTCTAG TACAGAGTTC GAAGGGATAG CCCAGCTGCC AAACACTGTG 
AAAAGGATCC ATCCCCAGAA AGGACAACAG GAGCCTCGCA TACAGCCCGG TTCTGAGCTA 
TCTGAAGAGG AAGCCTTCCT GAAGGGTCCT GCTCTGCAGC TGGTAGCCAC TGTGGAGAAG 
GATCCTGGCC CCTCAGTGTC CTCCCCTGTT CTGAAAAGCA AGCCTCGGCC TTGCCCTGCC 
TTCCTTTCCC ACTCGCTTTC CTGCAGCTGC ATTCTTTGTA CTGACCCTGC TCTGGTAGTT 
GTCTGCCTTC GGTGGCTGCT AGTCTCTGCC GGGGCAGAAC TGTCCTCCGG TCATCAGGCC 
AAAGGCCTGG ACATGTTACG GTCTGTGCTC AGGAGATGCC CAGGGGCCAC ACATCATCTG 
GCACAGGTTT TACAAGCTAC TTTGAGCCAA GAAGCATCCC CCTCACCTGC TCCAGGATTC 
TTAGAGGACC TCATTGCTCA GGCTTATGCT CATTTAGCCC TAGAAGGCAT AAACCATCCA 
CCGGACAAAG GCCTCTGGGA CATCCTTGAG ACTGGCCTGA ATTTTGTTGC TTCCAGGACT 
CCTTTCCTGA ACCCCTGGCA TGCCAGCCTT CTTGTGACAA AGAGCCTGGC AACACTATCC 
AGTCTCAGCT GTTGTACCAC TCAGCTCTTT GGGAACACTT GGGCCTGGCA ACCTCCAGCA 
GCAAAGCTCT CCCAGATGTC AAAGTCTTCT AAAAATCAGA GCCAAAATGG TCCAGGAGAA 
AGAGGCAAAC AAACGGCCTC TAGTGACTTG GCCCTCCACA CTCCCTCCCC AGAGGAGCTG 
GGTGAGAAGC CTTCAGCCGG GCCAAAGCCC ATCAGGAAGC CTTGTACCCC GAAGCAGCCA 
GGTCAGGCTG GCCCCCATGC ATCCTTCACA GTGTTTGATG AGGAACTTTC TAGCCAAGGC 
AAATCCGATT TAGCCAAGGC CCCGAGGGTG AGGTGCAGAG TCCAAACTCG TCTGAAGGTG 
AACTTTAGCG ATGACGATGA AGATCGTCCG ACCCCTTCCC ATGGACTGGC TGAAAAACCC 
AAGAGACGAG GAGCTGCCCC TCGAGGCAGG GGGAGGGGGG GCAGGGCCTT GAGGCTGAAG 
ACGGATGCCC AAGGTGATCC AGGGGGCTCT GGGGACCAAT CTGCCTGTAT CAGGCGAACT 
CGCCAGAGCC ACAAAGCGGG GGTCGAGGAG CAAGTCCCTC AGAGGGCCCT TCGACGGCAG 
GCCAATGTAG ACGTGGAGCT CATGAGGACC ATTCCTGAAG AACAGACAAC CACGAAACTG 
GACATGAGTG TGGAGATGCT GCAAGATTCC GATGAGGGAG GCAGAGCCTC AGGACAGAAT 
GCCTGGCAGG ACACTGCCAT GGGAGAGTGT GAAGTGGTGA GACGAGATGC CAGCAAGGAA 
GTGCCACCTT TCCCACCCCT AGAGAAGAGG GAAGGTGATC GAAGTCAGCA TGGTCCCCAG 
ACCAGGCCTG CATCTACCCC GGCAGCCTTT GGCCAGCCCA CCCTCGAATC CATCTATGAC 
TCCCTGAGCA TTGCCTTCCG CTGTGTCAGC CACTGCCCCC CGAGTGAGCT CTATGCTCAG 
CTGTGTCGTC TCCTTGCTCT GTGCCTGGGA CACCGGGACC CCCACACCAC TGCCTTGTTG 
GTCACTGAGT CTATGTCCAT CACCTCTCGG CACCAGCTGC TCACCACCGT GCACCGTCAT 
CTCCACAAAG CCCAGAAGCG GCAAGCAGCA GATGTGGTGG AGCAGCTCCA GGGCCTGAGC 
CTACAGGAAG AGCCCAGAGG GGTCCCCTTT GGCCGCCTGC AGCACCTCTT TTCATTCAGG 
ACACCCACCA GTGGCTGCTT CCCGCAGCCC GAGAAGGAGA GCTTCCAGGA GCTTCTGGCT 
CAGATGCCTA GCGGAGTGAC AGTGTGTGTG CTGGCTTTGG TCAGCCTCCA GTCTGGAACT 
GTCAGGGATA CATTACTATT GACACGGCTG GAAAAGAACA CTCCCCCCGT CACCATTCAG 
ATACCCACTG CCCAAACAAA GCTGCCTCTG AGTTCGGCCT TGGAAGAGTT TAATTTAATC 
CAGAAAGAAC AGAAGGAGAA TAGCAACTGT ACAGAGAAGT CCAAGTGGTG GGCAACTCGG 
CTGAAACTGG ATCGAAGGAT GGAGGTATTT GGTTGGGGCC CTTCTTTCCA CAGGGAATTT 
CCATCATCTT CTCTTTATGT GTCTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_007506530.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007506592.1 Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OMc36355
Clone ID Related Accession (Same CDS sequence)	XM_007506530.2
Accession Version	XM_007506530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001584931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007506530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG TGGGGATGGT GGTGAAGGTG TTATGCACAA TATGGGTCCC AAGACAGAAG 
CTCATATGGA CCATGAGGAA CTTTAAAGGA GCAGACTTTG GAAAATGGAT GAGCAGCCAA 
GAGGAGGCCA AGGCTTTGCT GGTTGAGCTG AAGGAGTTCC TGTCCAGACC TCTGGATAGC 
CCCACGGGCC GTTCATTTGC AGCAGAACAA AGACTTGCCT GTGATTCCAT TTTGAGGGCT 
TGCAACCAAC AGGTGGTGGC TGGGCTTGCT TGTCCTGACC ACTTGGGCAG CCTGTTGGAG 
CTGGCAGGGT TGGCCTGTGA TGGCTACTTG AAAGCAACCC CTCATCGCAC CCCACTCTAC 
TTAGAACGCA TCCTCTTCGT CTTCTTGCGA AATATTTTTG CTCGGGTGGT CCCAAACGCT 
GTTCTCTACC TGATCAAATC ATTGCATACC TGCCTAGTGC AGCATGTGCC AGTGGGTGCA 
ACCCAGGACT ATGAGGCTAT TATCCGAGGT GCCTTCTCTT TGCTTTGGAA AGGGGCAGAA 
GCACTTAGAG AACGTCAGCA AGCCTTCTCT GTTCGCCTGT GGGCCTTGAG TTTCTTGGTA 
CTCTTGGAAG ATGAAAGTAC CCCATGCAAA TTCCCCCTTT TTTCTTCCCC AACTGCTTGT 
CAAGCTGAAG CTGCCTGCCG GCTCTTTGAA GGCAGTGGGC ATCCTTTGGG TGAGGCAGAT 
GCCCACTTCC TCAGTGAACA GCTTTCTACT TGTGTGATCA AAGTGCTGGT TGGGAAGGAA 
GCCAGTCCTT CACATCCTCT CTCTCCCTGC AGGGCCCTCT GCCTCCTAGA GCTCACCTTA 
GAAAGGTGCC AAAGACTGTG CCTGAGCAGC TGCTGCACAG AGGCTGCTAG GGCTGTGCAG 
GAAGTCCAGG GTTATTTGGG GAACACAGCC CTGGCCCCTA GCCTCCAGCT GTGCCTGTTG 
GGGGTGGAAC TCCGAAGGCT GCTGGCTGGA GCCTTTGGGG GAGTGGGGGA ACTATTGCGC 
AAGGCCGAAG CTATTCTCAG CTTTGGCGTC AAGGGCCCGT CATCCTCATT TAGAGCTCTT 
GTGGAGACTT GTCAGTTCAT CCTCTTGGTC CTAGAGAGTG ATACTTTGAA AAAGTATGAA 
TTGGATAGCA TCCTGGGCCT CTCAGCCTTC TTGGGCAAAT ACTGTCATCT TCTTCGCCAG 
CTTCGGGACA GCGCTCCTGT GGATTGTCCC AAGCAGCAGC AAGTCTTGGC TCAGATGCAT 
TTTCATGGAC TCCAGCTCTT TACCAGGATG GTTTATGATT TCTTCAGTGG TTCTAAGGTC 
ACAGATTTGA CTGACTTGAC CGAGCTTGTT GAGAGCTGTC ATTGTGCTGT TGTCTGGATG 
TTGGAGGCTC TAGAGGGCCT GAAGGACCAA GAGCAGGCAG ACTACCTTGG AATCACTGCC 
TGCTGCACCA GTCACTTGGC CTATAAGTTC TACAACCAGA AGCTCTATGC TGAGGCCAGC 
ACCATCACTG AACCTTTCTG TCAGAAGATT GGACTGGCAA CATCTGGAAC ATACCCTGGG 
GTGCCTCCTG AGAAGCTGCA CAAGTGCTTC CGGTTACATG TAGAGAGCCT GAAGAAGCTA 
GGCCAGCCAG CCCAGGGATG CAGGATGGTG GCTCTGTGGC TGAAGGCCCT GCAGCCCTGC 
ACCCTCGAGC AAATGGCGGA GCCCATCAGT TTCTGGGTCC GTGTCAAGAT GGATGCCGCC 
AAGGCTGGTG ACAAGGAACT GCAGCTCAAG ACCCTTCGCG ATAGTCTGGC TGGTTGGGAC 
ACAGAGCTGC TGACACCCTT GCTGGTAGAA GAGTTGCGAA GCTATAAAGT GATCCGGGCC 
GACACAGGAC AAGAACGCTT CAATGTGATT TGTGACTTGT TGGAGCTGAG TACAGAAGAG 
ACACCAGCTG GGGCCTGGGC ACGGGCCACC TATTTGATAG AGCTTGCCCA AGTGCTGTGC 
TACTATGACT TTGCCAAGCA TGCCAACTGC TCAGCTTTGG AGGCTGTGCA GGAGGCCCTG 
AAACTGTTAG AGTCTGTACA GCCGGAAGGC CAGGCCACAG ACCAGTTTTT GGATGATAAG 
GCACAGGCTT TGCTGTGGCT TTACATCTGT AGCCTGGAGA CCAAAATGCA GAAAGGCATT 
GAACAGGACT TGAGGCTGAA GTTGCAGCCC AGCTCGATAG AATTGGAAAC CAATGACTTG 
AACTATGAGG ATAAAATCCA GGATGACCAT TTCCTCTACA GTAGCATTGC CTTCAATCTG 
GCTGTGGATG CTGCTCAGTC TAAATGTTTA GATCAGGCAC TGGCCCTTTG GAAGGAGATG 
CTAAAAAAAG GGCAGGTGCC CACCGTGAGA TGTCTACAAC AGACCACTGC CTCCCTCCAG 
ATTCTGGCTG CTCTCTATCA GATGGTGGCC AAGCCCCTAC AGGCTCTGGA GACACACTTG 
TTGCTCCGTA CTCTCTCAGA AAATGTTAAG GACTACATTA GAGCAGCCAG TGCCTCCTGC 
CATATCACTC GGCTCCTTCT GACACTAGAC TGTCCCGGCT ATGCTCAGTT CTACTTGGAG 
GAGGCAGAGT CCAGTCTGCA GCTCAGTGAT CACACCAGTG ATGGATACTT GCTCCACACC 
CAAACCTGTG CCCTACTTCG AAGTCAGCTT TACTGCCTCC AGAAAAAGGT GACTGAAGGC 
CTCTCTTTGT TGCTGTCTGT GGTACGGGAT TCAACCCTTC AAAAATCTTC TAAGGCTTGG 
TACTTGCTTC GGGTCCAGGC TTTGCAGGTG GTGGCTGTCT ACCTTAGTCT TCCCTCGAGC 
AGTCTTTCTG TCCAGCAGTG GGAGCAACTT TATACTCAAG GCTGGCAAAC CTCTGAGATA 
GCTCTCATTG AGTCCCACAA GCTCCTTCGA AGCATTATCA TCCTTCTGAT GGGCAGAGAC 
TTGCTCTTTG TTCAGCAGTC AGCAGTGGAC GCTGCCTTTC TTGACTATGG TGAGAACCTA 
GTACAGAAGT GGCAAGTTCT TGCAGAGGTC CTGGGATGCT CAGAGAAGCT GGTAACCTTG 
TTGGGACGCC TGGGCACTGT AAGTGAAGCC AAGGCCTTCT GCCTGGAGGC ACTGAGGCTT 
GCCATGAAGC TGCAGTGTGT TCGCCAGTGT TCCCTGTTCC TGGTGCTAAA GGGAGAACTA 
GAACTAGCCC GAAATGATCT TGACCTTTGC CAGTCGGACT TAGAGCAAGT GCTGTTTCTG 
CTTGGGTCTA GTACAGAGTT CGAAGGGATA GCCCAGCTGC CAAACACTGT GAAAAGGATC 
CATCCCCAGA AAGGACAACA GGAGCCTCGC ATACAGCCCG GTTCTGAGCT ATCTGAAGAG 
GAAGCCTTCC TGAAGGGTCC TGCTCTGCAG CTGGTAGCCA CTGTGGAGAA GGATCCTGGC 
CCCTCAGTGT CCTCCCCTGT TCTGAAAAGC AAGCCTCGGC CTTGCCCTGC CTTCCTTTCC 
CACTCGCTTT CCTGCAGCTG CATTCTTTGT ACTGACCCTG CTCTGGTAGT TGTCTGCCTT 
CGGTGGCTGC TAGTCTCTGC CGGGGCAGAA CTGTCCTCCG GTCATCAGGC CAAAGGCCTG 
GACATGTTAC GGTCTGTGCT CAGGAGATGC CCAGGGGCCA CACATCATCT GGCACAGGTT 
TTACAAGCTA CTTTGAGCCA AGAAGCATCC CCCTCACCTG CTCCAGGATT CTTAGAGGAC 
CTCATTGCTC AGGCTTATGC TCATTTAGCC CTAGAAGGCA TAAACCATCC ACCGGACAAA 
GGCCTCTGGG ACATCCTTGA GACTGGCCTG AATTTTGTTG CTTCCAGGAC TCCTTTCCTG 
AACCCCTGGC ATGCCAGCCT TCTTGTGACA AAGAGCCTGG CAACACTATC CAGTCTCAGC 
TGTTGTACCA CTCAGCTCTT TGGGAACACT TGGGCCTGGC AACCTCCAGC AGCAAAGCTC 
TCCCAGATGT CAAAGTCTTC TAAAAATCAG AGCCAAAATG GTCCAGGAGA AAGAGGCAAA 
CAAACGGCCT CTAGTGACTT GGCCCTCCAC ACTCCCTCCC CAGAGGAGCT GGGTGAGAAG 
CCTTCAGCCG GGCCAAAGCC CATCAGGAAG CCTTGTACCC CGAAGCAGCC AGGTCAGGCT 
GGCCCCCATG CATCCTTCAC AGTGTTTGAT GAGGAACTTT CTAGCCAAGG CAAATCCGAT 
TTAGCCAAGG CCCCGAGGGT GAGGTGCAGA GTCCAAACTC GTCTGAAGGT GAACTTTAGC 
GATGACGATG AAGATCGTCC GACCCCTTCC CATGGACTGG CTGAAAAACC CAAGAGACGA 
GGAGCTGCCC CTCGAGGCAG GGGGAGGGGG GGCAGGGCCT TGAGGCTGAA GACGGATGCC 
CAAGGTGATC CAGGGGGCTC TGGGGACCAA TCTGCCTGTA TCAGGCGAAC TCGCCAGAGC 
CACAAAGCGG GGGTCGAGGA GCAAGTCCCT CAGAGGGCCC TTCGACGGCA GGCCAATGTA 
GACGTGGAGC TCATGAGGAC CATTCCTGAA GAACAGACAA CCACGAAACT GGACATGAGT 
GTGGAGATGC TGCAAGATTC CGATGAGGGA GGCAGAGCCT CAGCAGGACA GAATGCCTGG 
CAGGACACTG CCATGGGAGA GTGTGAAGTG GTGAGACGAG ATGCCAGCAA GGAAGTGCCA 
CCTTTCCCAC CCCTAGAGAA GAGGGAAGGT GATCGAAGTC AGCATGGTCC CCAGACCAGG 
CCTGCATCTA CCCCGGCAGC CTTTGGCCAG CCCACCCTCG AATCCATCTA TGACTCCCTG 
AGCATTGCCT TCCGCTGTGT CAGCCACTGC CCCCCGAGTG AGCTCTATGC TCAGCTGTGT 
CGTCTCCTTG CTCTGTGCCT GGGACACCGG GACCCCCACA CCACTGCCTT GTTGGTCACT 
GAGTCTATGT CCATCACCTC TCGGCACCAG CTGCTCACCA CCGTGCACCG TCATCTCCAC 
AAAGCCCAGA AGCGGCAAGC AGCAGATGTG GTGGAGCAGC TCCAGGGCCT GAGCCTACAG 
GAAGAGCCCA GAGGGGTCCC CTTTGGCCGC CTGCAGCACC TCTTTTCATT CAGGACACCC 
ACCAGTGGCT GCTTCCCGCA GCCCGAGAAG GAGAGCTTCC AGGAGCTTCT GGCTCAGATG 
CCTAGCGGAG TGACAGTGTG TGTGCTGGCT TTGGTCAGCC TCCAGTCTGG AACTGTCAGG 
GATACATTAC TATTGACACG GCTGGAAAAG AACACTCCCC CCGTCACCAT TCAGATACCC 
ACTGCCCAAA CAAAGCTGCC TCTGAGTTCG GCCTTGGAAG AGTTTAATTT AATCCAGAAA 
GAACAGAAGG AGAATAGCAA CTGTACAGAG AAGTCCAAGT GGTGGGCAAC TCGGCTGAAA 
CTGGATCGAA GGATGGAGGT ATTTGGTTGG GGCCCTTCTT TCCACAGGGA ATTTCCATCA 
TCTTCTCTTT ATGTGTCTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007506592.1
CDS	207..5690
Translation	MSGVGMVVKVLCTIWVPRQKLIWTMRNFKGADFGKWMSSQEEAKALLVELKEFLSRPLDSPTGRSFAAEQRLACDSILRACNQQVVAGLACPDHLGSLLELAGLACDGYLKATPHRTPLYLERILFVFLRNIFARVVPNAVLYLIKSLHTCLVQHVPVGATQDYEAIIRGAFSLLWKGAEALRERQQAFSVRLWALSFLVLLEDESTPCKFPLFSSPTACQAEAACRLFEGSGHPLGEADAHFLSEQLSTCVIKVLVGKEASPSHPLSPCRALCLLELTLERCQRLCLSSCCTEAARAVQEVQGYLGNTALAPSLQLCLLGVELRRLLAGAFGGVGELLRKAEAILSFGVKGPSSSFRALVETCQFILLVLESDTLKKYELDSILGLSAFLGKYCHLLRQLRDSAPVDCPKQQQVLAQMHFHGLQLFTRMVYDFFSGSKVTDLTDLTELVESCHCAVVWMLEALEGLKDQEQADYLGITACCTSHLAYKFYNQKLYAEASTITEPFCQKIGLATSGTYPGVPPEKLHKCFRLHVESLKKLGQPAQGCRMVALWLKALQPCTLEQMAEPISFWVRVKMDAAKAGDKELQLKTLRDSLAGWDTELLTPLLVEELRSYKVIRADTGQERFNVICDLLELSTEETPAGAWARATYLIELAQVLCYYDFAKHANCSALEAVQEALKLLESVQPEGQATDQFLDDKAQALLWLYICSLETKMQKGIEQDLRLKLQPSSIELETNDLNYEDKIQDDHFLYSSIAFNLAVDAAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASLQILAALYQMVAKPLQALETHLLLRTLSENVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGYLLHTQTCALLRSQLYCLQKKVTEGLSLLLSVVRDSTLQKSSKAWYLLRVQALQVVAVYLSLPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLRSIIILLMGRDLLFVQQSAVDAAFLDYGENLVQKWQVLAEVLGCSEKLVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQCSLFLVLKGELELARNDLDLCQSDLEQVLFLLGSSTEFEGIAQLPNTVKRIHPQKGQQEPRIQPGSELSEEEAFLKGPALQLVATVEKDPGPSVSSPVLKSKPRPCPAFLSHSLSCSCILCTDPALVVVCLRWLLVSAGAELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQATLSQEASPSPAPGFLEDLIAQAYAHLALEGINHPPDKGLWDILETGLNFVASRTPFLNPWHASLLVTKSLATLSSLSCCTTQLFGNTWAWQPPAAKLSQMSKSSKNQSQNGPGERGKQTASSDLALHTPSPEELGEKPSAGPKPIRKPCTPKQPGQAGPHASFTVFDEELSSQGKSDLAKAPRVRCRVQTRLKVNFSDDDEDRPTPSHGLAEKPKRRGAAPRGRGRGGRALRLKTDAQGDPGGSGDQSACIRRTRQSHKAGVEEQVPQRALRRQANVDVELMRTIPEEQTTTKLDMSVEMLQDSDEGGRASAGQNAWQDTAMGECEVVRRDASKEVPPFPPLEKREGDRSQHGPQTRPASTPAAFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLGHRDPHTTALLVTESMSITSRHQLLTTVHRHLHKAQKRQAADVVEQLQGLSLQEEPRGVPFGRLQHLFSFRTPTSGCFPQPEKESFQELLAQMPSGVTVCVLALVSLQSGTVRDTLLLTRLEKNTPPVTIQIPTAQTKLPLSSALEEFNLIQKEQKENSNCTEKSKWWATRLKLDRRMEVFGWGPSFHREFPSSSLYVSF

Target ORF information:

RefSeq Version	XM_007506530.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007506530.2

ORF Insert Sequence:

ATGTCGGGGG TGGGGATGGT GGTGAAGGTG TTATGCACAA TATGGGTCCC AAGACAGAAG 
CTCATATGGA CCATGAGGAA CTTTAAAGGA GCAGACTTTG GAAAATGGAT GAGCAGCCAA 
GAGGAGGCCA AGGCTTTGCT GGTTGAGCTG AAGGAGTTCC TGTCCAGACC TCTGGATAGC 
CCCACGGGCC GTTCATTTGC AGCAGAACAA AGACTTGCCT GTGATTCCAT TTTGAGGGCT 
TGCAACCAAC AGGTGGTGGC TGGGCTTGCT TGTCCTGACC ACTTGGGCAG CCTGTTGGAG 
CTGGCAGGGT TGGCCTGTGA TGGCTACTTG AAAGCAACCC CTCATCGCAC CCCACTCTAC 
TTAGAACGCA TCCTCTTCGT CTTCTTGCGA AATATTTTTG CTCGGGTGGT CCCAAACGCT 
GTTCTCTACC TGATCAAATC ATTGCATACC TGCCTAGTGC AGCATGTGCC AGTGGGTGCA 
ACCCAGGACT ATGAGGCTAT TATCCGAGGT GCCTTCTCTT TGCTTTGGAA AGGGGCAGAA 
GCACTTAGAG AACGTCAGCA AGCCTTCTCT GTTCGCCTGT GGGCCTTGAG TTTCTTGGTA 
CTCTTGGAAG ATGAAAGTAC CCCATGCAAA TTCCCCCTTT TTTCTTCCCC AACTGCTTGT 
CAAGCTGAAG CTGCCTGCCG GCTCTTTGAA GGCAGTGGGC ATCCTTTGGG TGAGGCAGAT 
GCCCACTTCC TCAGTGAACA GCTTTCTACT TGTGTGATCA AAGTGCTGGT TGGGAAGGAA 
GCCAGTCCTT CACATCCTCT CTCTCCCTGC AGGGCCCTCT GCCTCCTAGA GCTCACCTTA 
GAAAGGTGCC AAAGACTGTG CCTGAGCAGC TGCTGCACAG AGGCTGCTAG GGCTGTGCAG 
GAAGTCCAGG GTTATTTGGG GAACACAGCC CTGGCCCCTA GCCTCCAGCT GTGCCTGTTG 
GGGGTGGAAC TCCGAAGGCT GCTGGCTGGA GCCTTTGGGG GAGTGGGGGA ACTATTGCGC 
AAGGCCGAAG CTATTCTCAG CTTTGGCGTC AAGGGCCCGT CATCCTCATT TAGAGCTCTT 
GTGGAGACTT GTCAGTTCAT CCTCTTGGTC CTAGAGAGTG ATACTTTGAA AAAGTATGAA 
TTGGATAGCA TCCTGGGCCT CTCAGCCTTC TTGGGCAAAT ACTGTCATCT TCTTCGCCAG 
CTTCGGGACA GCGCTCCTGT GGATTGTCCC AAGCAGCAGC AAGTCTTGGC TCAGATGCAT 
TTTCATGGAC TCCAGCTCTT TACCAGGATG GTTTATGATT TCTTCAGTGG TTCTAAGGTC 
ACAGATTTGA CTGACTTGAC CGAGCTTGTT GAGAGCTGTC ATTGTGCTGT TGTCTGGATG 
TTGGAGGCTC TAGAGGGCCT GAAGGACCAA GAGCAGGCAG ACTACCTTGG AATCACTGCC 
TGCTGCACCA GTCACTTGGC CTATAAGTTC TACAACCAGA AGCTCTATGC TGAGGCCAGC 
ACCATCACTG AACCTTTCTG TCAGAAGATT GGACTGGCAA CATCTGGAAC ATACCCTGGG 
GTGCCTCCTG AGAAGCTGCA CAAGTGCTTC CGGTTACATG TAGAGAGCCT GAAGAAGCTA 
GGCCAGCCAG CCCAGGGATG CAGGATGGTG GCTCTGTGGC TGAAGGCCCT GCAGCCCTGC 
ACCCTCGAGC AAATGGCGGA GCCCATCAGT TTCTGGGTCC GTGTCAAGAT GGATGCCGCC 
AAGGCTGGTG ACAAGGAACT GCAGCTCAAG ACCCTTCGCG ATAGTCTGGC TGGTTGGGAC 
ACAGAGCTGC TGACACCCTT GCTGGTAGAA GAGTTGCGAA GCTATAAAGT GATCCGGGCC 
GACACAGGAC AAGAACGCTT CAATGTGATT TGTGACTTGT TGGAGCTGAG TACAGAAGAG 
ACACCAGCTG GGGCCTGGGC ACGGGCCACC TATTTGATAG AGCTTGCCCA AGTGCTGTGC 
TACTATGACT TTGCCAAGCA TGCCAACTGC TCAGCTTTGG AGGCTGTGCA GGAGGCCCTG 
AAACTGTTAG AGTCTGTACA GCCGGAAGGC CAGGCCACAG ACCAGTTTTT GGATGATAAG 
GCACAGGCTT TGCTGTGGCT TTACATCTGT AGCCTGGAGA CCAAAATGCA GAAAGGCATT 
GAACAGGACT TGAGGCTGAA GTTGCAGCCC AGCTCGATAG AATTGGAAAC CAATGACTTG 
AACTATGAGG ATAAAATCCA GGATGACCAT TTCCTCTACA GTAGCATTGC CTTCAATCTG 
GCTGTGGATG CTGCTCAGTC TAAATGTTTA GATCAGGCAC TGGCCCTTTG GAAGGAGATG 
CTAAAAAAAG GGCAGGTGCC CACCGTGAGA TGTCTACAAC AGACCACTGC CTCCCTCCAG 
ATTCTGGCTG CTCTCTATCA GATGGTGGCC AAGCCCCTAC AGGCTCTGGA GACACACTTG 
TTGCTCCGTA CTCTCTCAGA AAATGTTAAG GACTACATTA GAGCAGCCAG TGCCTCCTGC 
CATATCACTC GGCTCCTTCT GACACTAGAC TGTCCCGGCT ATGCTCAGTT CTACTTGGAG 
GAGGCAGAGT CCAGTCTGCA GCTCAGTGAT CACACCAGTG ATGGATACTT GCTCCACACC 
CAAACCTGTG CCCTACTTCG AAGTCAGCTT TACTGCCTCC AGAAAAAGGT GACTGAAGGC 
CTCTCTTTGT TGCTGTCTGT GGTACGGGAT TCAACCCTTC AAAAATCTTC TAAGGCTTGG 
TACTTGCTTC GGGTCCAGGC TTTGCAGGTG GTGGCTGTCT ACCTTAGTCT TCCCTCGAGC 
AGTCTTTCTG TCCAGCAGTG GGAGCAACTT TATACTCAAG GCTGGCAAAC CTCTGAGATA 
GCTCTCATTG AGTCCCACAA GCTCCTTCGA AGCATTATCA TCCTTCTGAT GGGCAGAGAC 
TTGCTCTTTG TTCAGCAGTC AGCAGTGGAC GCTGCCTTTC TTGACTATGG TGAGAACCTA 
GTACAGAAGT GGCAAGTTCT TGCAGAGGTC CTGGGATGCT CAGAGAAGCT GGTAACCTTG 
TTGGGACGCC TGGGCACTGT AAGTGAAGCC AAGGCCTTCT GCCTGGAGGC ACTGAGGCTT 
GCCATGAAGC TGCAGTGTGT TCGCCAGTGT TCCCTGTTCC TGGTGCTAAA GGGAGAACTA 
GAACTAGCCC GAAATGATCT TGACCTTTGC CAGTCGGACT TAGAGCAAGT GCTGTTTCTG 
CTTGGGTCTA GTACAGAGTT CGAAGGGATA GCCCAGCTGC CAAACACTGT GAAAAGGATC 
CATCCCCAGA AAGGACAACA GGAGCCTCGC ATACAGCCCG GTTCTGAGCT ATCTGAAGAG 
GAAGCCTTCC TGAAGGGTCC TGCTCTGCAG CTGGTAGCCA CTGTGGAGAA GGATCCTGGC 
CCCTCAGTGT CCTCCCCTGT TCTGAAAAGC AAGCCTCGGC CTTGCCCTGC CTTCCTTTCC 
CACTCGCTTT CCTGCAGCTG CATTCTTTGT ACTGACCCTG CTCTGGTAGT TGTCTGCCTT 
CGGTGGCTGC TAGTCTCTGC CGGGGCAGAA CTGTCCTCCG GTCATCAGGC CAAAGGCCTG 
GACATGTTAC GGTCTGTGCT CAGGAGATGC CCAGGGGCCA CACATCATCT GGCACAGGTT 
TTACAAGCTA CTTTGAGCCA AGAAGCATCC CCCTCACCTG CTCCAGGATT CTTAGAGGAC 
CTCATTGCTC AGGCTTATGC TCATTTAGCC CTAGAAGGCA TAAACCATCC ACCGGACAAA 
GGCCTCTGGG ACATCCTTGA GACTGGCCTG AATTTTGTTG CTTCCAGGAC TCCTTTCCTG 
AACCCCTGGC ATGCCAGCCT TCTTGTGACA AAGAGCCTGG CAACACTATC CAGTCTCAGC 
TGTTGTACCA CTCAGCTCTT TGGGAACACT TGGGCCTGGC AACCTCCAGC AGCAAAGCTC 
TCCCAGATGT CAAAGTCTTC TAAAAATCAG AGCCAAAATG GTCCAGGAGA AAGAGGCAAA 
CAAACGGCCT CTAGTGACTT GGCCCTCCAC ACTCCCTCCC CAGAGGAGCT GGGTGAGAAG 
CCTTCAGCCG GGCCAAAGCC CATCAGGAAG CCTTGTACCC CGAAGCAGCC AGGTCAGGCT 
GGCCCCCATG CATCCTTCAC AGTGTTTGAT GAGGAACTTT CTAGCCAAGG CAAATCCGAT 
TTAGCCAAGG CCCCGAGGGT GAGGTGCAGA GTCCAAACTC GTCTGAAGGT GAACTTTAGC 
GATGACGATG AAGATCGTCC GACCCCTTCC CATGGACTGG CTGAAAAACC CAAGAGACGA 
GGAGCTGCCC CTCGAGGCAG GGGGAGGGGG GGCAGGGCCT TGAGGCTGAA GACGGATGCC 
CAAGGTGATC CAGGGGGCTC TGGGGACCAA TCTGCCTGTA TCAGGCGAAC TCGCCAGAGC 
CACAAAGCGG GGGTCGAGGA GCAAGTCCCT CAGAGGGCCC TTCGACGGCA GGCCAATGTA 
GACGTGGAGC TCATGAGGAC CATTCCTGAA GAACAGACAA CCACGAAACT GGACATGAGT 
GTGGAGATGC TGCAAGATTC CGATGAGGGA GGCAGAGCCT CAGCAGGACA GAATGCCTGG 
CAGGACACTG CCATGGGAGA GTGTGAAGTG GTGAGACGAG ATGCCAGCAA GGAAGTGCCA 
CCTTTCCCAC CCCTAGAGAA GAGGGAAGGT GATCGAAGTC AGCATGGTCC CCAGACCAGG 
CCTGCATCTA CCCCGGCAGC CTTTGGCCAG CCCACCCTCG AATCCATCTA TGACTCCCTG 
AGCATTGCCT TCCGCTGTGT CAGCCACTGC CCCCCGAGTG AGCTCTATGC TCAGCTGTGT 
CGTCTCCTTG CTCTGTGCCT GGGACACCGG GACCCCCACA CCACTGCCTT GTTGGTCACT 
GAGTCTATGT CCATCACCTC TCGGCACCAG CTGCTCACCA CCGTGCACCG TCATCTCCAC 
AAAGCCCAGA AGCGGCAAGC AGCAGATGTG GTGGAGCAGC TCCAGGGCCT GAGCCTACAG 
GAAGAGCCCA GAGGGGTCCC CTTTGGCCGC CTGCAGCACC TCTTTTCATT CAGGACACCC 
ACCAGTGGCT GCTTCCCGCA GCCCGAGAAG GAGAGCTTCC AGGAGCTTCT GGCTCAGATG 
CCTAGCGGAG TGACAGTGTG TGTGCTGGCT TTGGTCAGCC TCCAGTCTGG AACTGTCAGG 
GATACATTAC TATTGACACG GCTGGAAAAG AACACTCCCC CCGTCACCAT TCAGATACCC 
ACTGCCCAAA CAAAGCTGCC TCTGAGTTCG GCCTTGGAAG AGTTTAATTT AATCCAGAAA 
GAACAGAAGG AGAATAGCAA CTGTACAGAG AAGTCCAAGT GGTGGGCAAC TCGGCTGAAA 
CTGGATCGAA GGATGGAGGT ATTTGGTTGG GGCCCTTCTT TCCACAGGGA ATTTCCATCA 
TCTTCTCTTT ATGTGTCTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_007506532.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007506594.1 Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

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CloneID	OMc36356
Clone ID Related Accession (Same CDS sequence)	XM_007506532.2 , XM_007506531.2
Accession Version	XM_007506532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	separin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001584931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007506532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT TTAAAGGAGC AGACTTTGGA AAATGGATGA GCAGCCAAGA GGAGGCCAAG 
GCTTTGCTGG TTGAGCTGAA GGAGTTCCTG TCCAGACCTC TGGATAGCCC CACGGGCCGT 
TCATTTGCAG CAGAACAAAG ACTTGCCTGT GATTCCATTT TGAGGGCTTG CAACCAACAG 
GTGGTGGCTG GGCTTGCTTG TCCTGACCAC TTGGGCAGCC TGTTGGAGCT GGCAGGGTTG 
GCCTGTGATG GCTACTTGAA AGCAACCCCT CATCGCACCC CACTCTACTT AGAACGCATC 
CTCTTCGTCT TCTTGCGAAA TATTTTTGCT CGGGTGGTCC CAAACGCTGT TCTCTACCTG 
ATCAAATCAT TGCATACCTG CCTAGTGCAG CATGTGCCAG TGGGTGCAAC CCAGGACTAT 
GAGGCTATTA TCCGAGGTGC CTTCTCTTTG CTTTGGAAAG GGGCAGAAGC ACTTAGAGAA 
CGTCAGCAAG CCTTCTCTGT TCGCCTGTGG GCCTTGAGTT TCTTGGTACT CTTGGAAGAT 
GAAAGTACCC CATGCAAATT CCCCCTTTTT TCTTCCCCAA CTGCTTGTCA AGCTGAAGCT 
GCCTGCCGGC TCTTTGAAGG CAGTGGGCAT CCTTTGGGTG AGGCAGATGC CCACTTCCTC 
AGTGAACAGC TTTCTACTTG TGTGATCAAA GTGCTGGTTG GGAAGGAAGC CAGTCCTTCA 
CATCCTCTCT CTCCCTGCAG GGCCCTCTGC CTCCTAGAGC TCACCTTAGA AAGGTGCCAA 
AGACTGTGCC TGAGCAGCTG CTGCACAGAG GCTGCTAGGG CTGTGCAGGA AGTCCAGGGT 
TATTTGGGGA ACACAGCCCT GGCCCCTAGC CTCCAGCTGT GCCTGTTGGG GGTGGAACTC 
CGAAGGCTGC TGGCTGGAGC CTTTGGGGGA GTGGGGGAAC TATTGCGCAA GGCCGAAGCT 
ATTCTCAGCT TTGGCGTCAA GGGCCCGTCA TCCTCATTTA GAGCTCTTGT GGAGACTTGT 
CAGTTCATCC TCTTGGTCCT AGAGAGTGAT ACTTTGAAAA AGTATGAATT GGATAGCATC 
CTGGGCCTCT CAGCCTTCTT GGGCAAATAC TGTCATCTTC TTCGCCAGCT TCGGGACAGC 
GCTCCTGTGG ATTGTCCCAA GCAGCAGCAA GTCTTGGCTC AGATGCATTT TCATGGACTC 
CAGCTCTTTA CCAGGATGGT TTATGATTTC TTCAGTGGTT CTAAGGTCAC AGATTTGACT 
GACTTGACCG AGCTTGTTGA GAGCTGTCAT TGTGCTGTTG TCTGGATGTT GGAGGCTCTA 
GAGGGCCTGA AGGACCAAGA GCAGGCAGAC TACCTTGGAA TCACTGCCTG CTGCACCAGT 
CACTTGGCCT ATAAGTTCTA CAACCAGAAG CTCTATGCTG AGGCCAGCAC CATCACTGAA 
CCTTTCTGTC AGAAGATTGG ACTGGCAACA TCTGGAACAT ACCCTGGGGT GCCTCCTGAG 
AAGCTGCACA AGTGCTTCCG GTTACATGTA GAGAGCCTGA AGAAGCTAGG CCAGCCAGCC 
CAGGGATGCA GGATGGTGGC TCTGTGGCTG AAGGCCCTGC AGCCCTGCAC CCTCGAGCAA 
ATGGCGGAGC CCATCAGTTT CTGGGTCCGT GTCAAGATGG ATGCCGCCAA GGCTGGTGAC 
AAGGAACTGC AGCTCAAGAC CCTTCGCGAT AGTCTGGCTG GTTGGGACAC AGAGCTGCTG 
ACACCCTTGC TGGTAGAAGA GTTGCGAAGC TATAAAGTGA TCCGGGCCGA CACAGGACAA 
GAACGCTTCA ATGTGATTTG TGACTTGTTG GAGCTGAGTA CAGAAGAGAC ACCAGCTGGG 
GCCTGGGCAC GGGCCACCTA TTTGATAGAG CTTGCCCAAG TGCTGTGCTA CTATGACTTT 
GCCAAGCATG CCAACTGCTC AGCTTTGGAG GCTGTGCAGG AGGCCCTGAA ACTGTTAGAG 
TCTGTACAGC CGGAAGGCCA GGCCACAGAC CAGTTTTTGG ATGATAAGGC ACAGGCTTTG 
CTGTGGCTTT ACATCTGTAG CCTGGAGACC AAAATGCAGA AAGGCATTGA ACAGGACTTG 
AGGCTGAAGT TGCAGCCCAG CTCGATAGAA TTGGAAACCA ATGACTTGAA CTATGAGGAT 
AAAATCCAGG ATGACCATTT CCTCTACAGT AGCATTGCCT TCAATCTGGC TGTGGATGCT 
GCTCAGTCTA AATGTTTAGA TCAGGCACTG GCCCTTTGGA AGGAGATGCT AAAAAAAGGG 
CAGGTGCCCA CCGTGAGATG TCTACAACAG ACCACTGCCT CCCTCCAGAT TCTGGCTGCT 
CTCTATCAGA TGGTGGCCAA GCCCCTACAG GCTCTGGAGA CACACTTGTT GCTCCGTACT 
CTCTCAGAAA ATGTTAAGGA CTACATTAGA GCAGCCAGTG CCTCCTGCCA TATCACTCGG 
CTCCTTCTGA CACTAGACTG TCCCGGCTAT GCTCAGTTCT ACTTGGAGGA GGCAGAGTCC 
AGTCTGCAGC TCAGTGATCA CACCAGTGAT GGATACTTGC TCCACACCCA AACCTGTGCC 
CTACTTCGAA GTCAGCTTTA CTGCCTCCAG AAAAAGGTGA CTGAAGGCCT CTCTTTGTTG 
CTGTCTGTGG TACGGGATTC AACCCTTCAA AAATCTTCTA AGGCTTGGTA CTTGCTTCGG 
GTCCAGGCTT TGCAGGTGGT GGCTGTCTAC CTTAGTCTTC CCTCGAGCAG TCTTTCTGTC 
CAGCAGTGGG AGCAACTTTA TACTCAAGGC TGGCAAACCT CTGAGATAGC TCTCATTGAG 
TCCCACAAGC TCCTTCGAAG CATTATCATC CTTCTGATGG GCAGAGACTT GCTCTTTGTT 
CAGCAGTCAG CAGTGGACGC TGCCTTTCTT GACTATGGTG AGAACCTAGT ACAGAAGTGG 
CAAGTTCTTG CAGAGGTCCT GGGATGCTCA GAGAAGCTGG TAACCTTGTT GGGACGCCTG 
GGCACTGTAA GTGAAGCCAA GGCCTTCTGC CTGGAGGCAC TGAGGCTTGC CATGAAGCTG 
CAGTGTGTTC GCCAGTGTTC CCTGTTCCTG GTGCTAAAGG GAGAACTAGA ACTAGCCCGA 
AATGATCTTG ACCTTTGCCA GTCGGACTTA GAGCAAGTGC TGTTTCTGCT TGGGTCTAGT 
ACAGAGTTCG AAGGGATAGC CCAGCTGCCA AACACTGTGA AAAGGATCCA TCCCCAGAAA 
GGACAACAGG AGCCTCGCAT ACAGCCCGGT TCTGAGCTAT CTGAAGAGGA AGCCTTCCTG 
AAGGGTCCTG CTCTGCAGCT GGTAGCCACT GTGGAGAAGG ATCCTGGCCC CTCAGTGTCC 
TCCCCTGTTC TGAAAAGCAA GCCTCGGCCT TGCCCTGCCT TCCTTTCCCA CTCGCTTTCC 
TGCAGCTGCA TTCTTTGTAC TGACCCTGCT CTGGTAGTTG TCTGCCTTCG GTGGCTGCTA 
GTCTCTGCCG GGGCAGAACT GTCCTCCGGT CATCAGGCCA AAGGCCTGGA CATGTTACGG 
TCTGTGCTCA GGAGATGCCC AGGGGCCACA CATCATCTGG CACAGGTTTT ACAAGCTACT 
TTGAGCCAAG AAGCATCCCC CTCACCTGCT CCAGGATTCT TAGAGGACCT CATTGCTCAG 
GCTTATGCTC ATTTAGCCCT AGAAGGCATA AACCATCCAC CGGACAAAGG CCTCTGGGAC 
ATCCTTGAGA CTGGCCTGAA TTTTGTTGCT TCCAGGACTC CTTTCCTGAA CCCCTGGCAT 
GCCAGCCTTC TTGTGACAAA GAGCCTGGCA ACACTATCCA GTCTCAGCTG TTGTACCACT 
CAGCTCTTTG GGAACACTTG GGCCTGGCAA CCTCCAGCAG CAAAGCTCTC CCAGATGTCA 
AAGTCTTCTA AAAATCAGAG CCAAAATGGT CCAGGAGAAA GAGGCAAACA AACGGCCTCT 
AGTGACTTGG CCCTCCACAC TCCCTCCCCA GAGGAGCTGG GTGAGAAGCC TTCAGCCGGG 
CCAAAGCCCA TCAGGAAGCC TTGTACCCCG AAGCAGCCAG GTCAGGCTGG CCCCCATGCA 
TCCTTCACAG TGTTTGATGA GGAACTTTCT AGCCAAGGCA AATCCGATTT AGCCAAGGCC 
CCGAGGGTGA GGTGCAGAGT CCAAACTCGT CTGAAGGTGA ACTTTAGCGA TGACGATGAA 
GATCGTCCGA CCCCTTCCCA TGGACTGGCT GAAAAACCCA AGAGACGAGG AGCTGCCCCT 
CGAGGCAGGG GGAGGGGGGG CAGGGCCTTG AGGCTGAAGA CGGATGCCCA AGGTGATCCA 
GGGGGCTCTG GGGACCAATC TGCCTGTATC AGGCGAACTC GCCAGAGCCA CAAAGCGGGG 
GTCGAGGAGC AAGTCCCTCA GAGGGCCCTT CGACGGCAGG CCAATGTAGA CGTGGAGCTC 
ATGAGGACCA TTCCTGAAGA ACAGACAACC ACGAAACTGG ACATGAGTGT GGAGATGCTG 
CAAGATTCCG ATGAGGGAGG CAGAGCCTCA GCAGGACAGA ATGCCTGGCA GGACACTGCC 
ATGGGAGAGT GTGAAGTGGT GAGACGAGAT GCCAGCAAGG AAGTGCCACC TTTCCCACCC 
CTAGAGAAGA GGGAAGGTGA TCGAAGTCAG CATGGTCCCC AGACCAGGCC TGCATCTACC 
CCGGCAGCCT TTGGCCAGCC CACCCTCGAA TCCATCTATG ACTCCCTGAG CATTGCCTTC 
CGCTGTGTCA GCCACTGCCC CCCGAGTGAG CTCTATGCTC AGCTGTGTCG TCTCCTTGCT 
CTGTGCCTGG GACACCGGGA CCCCCACACC ACTGCCTTGT TGGTCACTGA GTCTATGTCC 
ATCACCTCTC GGCACCAGCT GCTCACCACC GTGCACCGTC ATCTCCACAA AGCCCAGAAG 
CGGCAAGCAG CAGATGTGGT GGAGCAGCTC CAGGGCCTGA GCCTACAGGA AGAGCCCAGA 
GGGGTCCCCT TTGGCCGCCT GCAGCACCTC TTTTCATTCA GGACACCCAC CAGTGGCTGC 
TTCCCGCAGC CCGAGAAGGA GAGCTTCCAG GAGCTTCTGG CTCAGATGCC TAGCGGAGTG 
ACAGTGTGTG TGCTGGCTTT GGTCAGCCTC CAGTCTGGAA CTGTCAGGGA TACATTACTA 
TTGACACGGC TGGAAAAGAA CACTCCCCCC GTCACCATTC AGATACCCAC TGCCCAAACA 
AAGCTGCCTC TGAGTTCGGC CTTGGAAGAG TTTAATTTAA TCCAGAAAGA ACAGAAGGAG 
AATAGCAACT GTACAGAGAA GTCCAAGTGG TGGGCAACTC GGCTGAAACT GGATCGAAGG 
ATGGAGGTAT TTGGTTGGGG CCCTTCTTTC CACAGGGAAT TTCCATCATC TTCTCTTTAT 
GTGTCTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007506594.1
CDS	73..5484
Translation	MRNFKGADFGKWMSSQEEAKALLVELKEFLSRPLDSPTGRSFAAEQRLACDSILRACNQQVVAGLACPDHLGSLLELAGLACDGYLKATPHRTPLYLERILFVFLRNIFARVVPNAVLYLIKSLHTCLVQHVPVGATQDYEAIIRGAFSLLWKGAEALRERQQAFSVRLWALSFLVLLEDESTPCKFPLFSSPTACQAEAACRLFEGSGHPLGEADAHFLSEQLSTCVIKVLVGKEASPSHPLSPCRALCLLELTLERCQRLCLSSCCTEAARAVQEVQGYLGNTALAPSLQLCLLGVELRRLLAGAFGGVGELLRKAEAILSFGVKGPSSSFRALVETCQFILLVLESDTLKKYELDSILGLSAFLGKYCHLLRQLRDSAPVDCPKQQQVLAQMHFHGLQLFTRMVYDFFSGSKVTDLTDLTELVESCHCAVVWMLEALEGLKDQEQADYLGITACCTSHLAYKFYNQKLYAEASTITEPFCQKIGLATSGTYPGVPPEKLHKCFRLHVESLKKLGQPAQGCRMVALWLKALQPCTLEQMAEPISFWVRVKMDAAKAGDKELQLKTLRDSLAGWDTELLTPLLVEELRSYKVIRADTGQERFNVICDLLELSTEETPAGAWARATYLIELAQVLCYYDFAKHANCSALEAVQEALKLLESVQPEGQATDQFLDDKAQALLWLYICSLETKMQKGIEQDLRLKLQPSSIELETNDLNYEDKIQDDHFLYSSIAFNLAVDAAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASLQILAALYQMVAKPLQALETHLLLRTLSENVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGYLLHTQTCALLRSQLYCLQKKVTEGLSLLLSVVRDSTLQKSSKAWYLLRVQALQVVAVYLSLPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLRSIIILLMGRDLLFVQQSAVDAAFLDYGENLVQKWQVLAEVLGCSEKLVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQCSLFLVLKGELELARNDLDLCQSDLEQVLFLLGSSTEFEGIAQLPNTVKRIHPQKGQQEPRIQPGSELSEEEAFLKGPALQLVATVEKDPGPSVSSPVLKSKPRPCPAFLSHSLSCSCILCTDPALVVVCLRWLLVSAGAELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQATLSQEASPSPAPGFLEDLIAQAYAHLALEGINHPPDKGLWDILETGLNFVASRTPFLNPWHASLLVTKSLATLSSLSCCTTQLFGNTWAWQPPAAKLSQMSKSSKNQSQNGPGERGKQTASSDLALHTPSPEELGEKPSAGPKPIRKPCTPKQPGQAGPHASFTVFDEELSSQGKSDLAKAPRVRCRVQTRLKVNFSDDDEDRPTPSHGLAEKPKRRGAAPRGRGRGGRALRLKTDAQGDPGGSGDQSACIRRTRQSHKAGVEEQVPQRALRRQANVDVELMRTIPEEQTTTKLDMSVEMLQDSDEGGRASAGQNAWQDTAMGECEVVRRDASKEVPPFPPLEKREGDRSQHGPQTRPASTPAAFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLGHRDPHTTALLVTESMSITSRHQLLTTVHRHLHKAQKRQAADVVEQLQGLSLQEEPRGVPFGRLQHLFSFRTPTSGCFPQPEKESFQELLAQMPSGVTVCVLALVSLQSGTVRDTLLLTRLEKNTPPVTIQIPTAQTKLPLSSALEEFNLIQKEQKENSNCTEKSKWWATRLKLDRRMEVFGWGPSFHREFPSSSLYVSF

Target ORF information:

RefSeq Version	XM_007506532.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007506532.2

ORF Insert Sequence:

ATGAGGAACT TTAAAGGAGC AGACTTTGGA AAATGGATGA GCAGCCAAGA GGAGGCCAAG 
GCTTTGCTGG TTGAGCTGAA GGAGTTCCTG TCCAGACCTC TGGATAGCCC CACGGGCCGT 
TCATTTGCAG CAGAACAAAG ACTTGCCTGT GATTCCATTT TGAGGGCTTG CAACCAACAG 
GTGGTGGCTG GGCTTGCTTG TCCTGACCAC TTGGGCAGCC TGTTGGAGCT GGCAGGGTTG 
GCCTGTGATG GCTACTTGAA AGCAACCCCT CATCGCACCC CACTCTACTT AGAACGCATC 
CTCTTCGTCT TCTTGCGAAA TATTTTTGCT CGGGTGGTCC CAAACGCTGT TCTCTACCTG 
ATCAAATCAT TGCATACCTG CCTAGTGCAG CATGTGCCAG TGGGTGCAAC CCAGGACTAT 
GAGGCTATTA TCCGAGGTGC CTTCTCTTTG CTTTGGAAAG GGGCAGAAGC ACTTAGAGAA 
CGTCAGCAAG CCTTCTCTGT TCGCCTGTGG GCCTTGAGTT TCTTGGTACT CTTGGAAGAT 
GAAAGTACCC CATGCAAATT CCCCCTTTTT TCTTCCCCAA CTGCTTGTCA AGCTGAAGCT 
GCCTGCCGGC TCTTTGAAGG CAGTGGGCAT CCTTTGGGTG AGGCAGATGC CCACTTCCTC 
AGTGAACAGC TTTCTACTTG TGTGATCAAA GTGCTGGTTG GGAAGGAAGC CAGTCCTTCA 
CATCCTCTCT CTCCCTGCAG GGCCCTCTGC CTCCTAGAGC TCACCTTAGA AAGGTGCCAA 
AGACTGTGCC TGAGCAGCTG CTGCACAGAG GCTGCTAGGG CTGTGCAGGA AGTCCAGGGT 
TATTTGGGGA ACACAGCCCT GGCCCCTAGC CTCCAGCTGT GCCTGTTGGG GGTGGAACTC 
CGAAGGCTGC TGGCTGGAGC CTTTGGGGGA GTGGGGGAAC TATTGCGCAA GGCCGAAGCT 
ATTCTCAGCT TTGGCGTCAA GGGCCCGTCA TCCTCATTTA GAGCTCTTGT GGAGACTTGT 
CAGTTCATCC TCTTGGTCCT AGAGAGTGAT ACTTTGAAAA AGTATGAATT GGATAGCATC 
CTGGGCCTCT CAGCCTTCTT GGGCAAATAC TGTCATCTTC TTCGCCAGCT TCGGGACAGC 
GCTCCTGTGG ATTGTCCCAA GCAGCAGCAA GTCTTGGCTC AGATGCATTT TCATGGACTC 
CAGCTCTTTA CCAGGATGGT TTATGATTTC TTCAGTGGTT CTAAGGTCAC AGATTTGACT 
GACTTGACCG AGCTTGTTGA GAGCTGTCAT TGTGCTGTTG TCTGGATGTT GGAGGCTCTA 
GAGGGCCTGA AGGACCAAGA GCAGGCAGAC TACCTTGGAA TCACTGCCTG CTGCACCAGT 
CACTTGGCCT ATAAGTTCTA CAACCAGAAG CTCTATGCTG AGGCCAGCAC CATCACTGAA 
CCTTTCTGTC AGAAGATTGG ACTGGCAACA TCTGGAACAT ACCCTGGGGT GCCTCCTGAG 
AAGCTGCACA AGTGCTTCCG GTTACATGTA GAGAGCCTGA AGAAGCTAGG CCAGCCAGCC 
CAGGGATGCA GGATGGTGGC TCTGTGGCTG AAGGCCCTGC AGCCCTGCAC CCTCGAGCAA 
ATGGCGGAGC CCATCAGTTT CTGGGTCCGT GTCAAGATGG ATGCCGCCAA GGCTGGTGAC 
AAGGAACTGC AGCTCAAGAC CCTTCGCGAT AGTCTGGCTG GTTGGGACAC AGAGCTGCTG 
ACACCCTTGC TGGTAGAAGA GTTGCGAAGC TATAAAGTGA TCCGGGCCGA CACAGGACAA 
GAACGCTTCA ATGTGATTTG TGACTTGTTG GAGCTGAGTA CAGAAGAGAC ACCAGCTGGG 
GCCTGGGCAC GGGCCACCTA TTTGATAGAG CTTGCCCAAG TGCTGTGCTA CTATGACTTT 
GCCAAGCATG CCAACTGCTC AGCTTTGGAG GCTGTGCAGG AGGCCCTGAA ACTGTTAGAG 
TCTGTACAGC CGGAAGGCCA GGCCACAGAC CAGTTTTTGG ATGATAAGGC ACAGGCTTTG 
CTGTGGCTTT ACATCTGTAG CCTGGAGACC AAAATGCAGA AAGGCATTGA ACAGGACTTG 
AGGCTGAAGT TGCAGCCCAG CTCGATAGAA TTGGAAACCA ATGACTTGAA CTATGAGGAT 
AAAATCCAGG ATGACCATTT CCTCTACAGT AGCATTGCCT TCAATCTGGC TGTGGATGCT 
GCTCAGTCTA AATGTTTAGA TCAGGCACTG GCCCTTTGGA AGGAGATGCT AAAAAAAGGG 
CAGGTGCCCA CCGTGAGATG TCTACAACAG ACCACTGCCT CCCTCCAGAT TCTGGCTGCT 
CTCTATCAGA TGGTGGCCAA GCCCCTACAG GCTCTGGAGA CACACTTGTT GCTCCGTACT 
CTCTCAGAAA ATGTTAAGGA CTACATTAGA GCAGCCAGTG CCTCCTGCCA TATCACTCGG 
CTCCTTCTGA CACTAGACTG TCCCGGCTAT GCTCAGTTCT ACTTGGAGGA GGCAGAGTCC 
AGTCTGCAGC TCAGTGATCA CACCAGTGAT GGATACTTGC TCCACACCCA AACCTGTGCC 
CTACTTCGAA GTCAGCTTTA CTGCCTCCAG AAAAAGGTGA CTGAAGGCCT CTCTTTGTTG 
CTGTCTGTGG TACGGGATTC AACCCTTCAA AAATCTTCTA AGGCTTGGTA CTTGCTTCGG 
GTCCAGGCTT TGCAGGTGGT GGCTGTCTAC CTTAGTCTTC CCTCGAGCAG TCTTTCTGTC 
CAGCAGTGGG AGCAACTTTA TACTCAAGGC TGGCAAACCT CTGAGATAGC TCTCATTGAG 
TCCCACAAGC TCCTTCGAAG CATTATCATC CTTCTGATGG GCAGAGACTT GCTCTTTGTT 
CAGCAGTCAG CAGTGGACGC TGCCTTTCTT GACTATGGTG AGAACCTAGT ACAGAAGTGG 
CAAGTTCTTG CAGAGGTCCT GGGATGCTCA GAGAAGCTGG TAACCTTGTT GGGACGCCTG 
GGCACTGTAA GTGAAGCCAA GGCCTTCTGC CTGGAGGCAC TGAGGCTTGC CATGAAGCTG 
CAGTGTGTTC GCCAGTGTTC CCTGTTCCTG GTGCTAAAGG GAGAACTAGA ACTAGCCCGA 
AATGATCTTG ACCTTTGCCA GTCGGACTTA GAGCAAGTGC TGTTTCTGCT TGGGTCTAGT 
ACAGAGTTCG AAGGGATAGC CCAGCTGCCA AACACTGTGA AAAGGATCCA TCCCCAGAAA 
GGACAACAGG AGCCTCGCAT ACAGCCCGGT TCTGAGCTAT CTGAAGAGGA AGCCTTCCTG 
AAGGGTCCTG CTCTGCAGCT GGTAGCCACT GTGGAGAAGG ATCCTGGCCC CTCAGTGTCC 
TCCCCTGTTC TGAAAAGCAA GCCTCGGCCT TGCCCTGCCT TCCTTTCCCA CTCGCTTTCC 
TGCAGCTGCA TTCTTTGTAC TGACCCTGCT CTGGTAGTTG TCTGCCTTCG GTGGCTGCTA 
GTCTCTGCCG GGGCAGAACT GTCCTCCGGT CATCAGGCCA AAGGCCTGGA CATGTTACGG 
TCTGTGCTCA GGAGATGCCC AGGGGCCACA CATCATCTGG CACAGGTTTT ACAAGCTACT 
TTGAGCCAAG AAGCATCCCC CTCACCTGCT CCAGGATTCT TAGAGGACCT CATTGCTCAG 
GCTTATGCTC ATTTAGCCCT AGAAGGCATA AACCATCCAC CGGACAAAGG CCTCTGGGAC 
ATCCTTGAGA CTGGCCTGAA TTTTGTTGCT TCCAGGACTC CTTTCCTGAA CCCCTGGCAT 
GCCAGCCTTC TTGTGACAAA GAGCCTGGCA ACACTATCCA GTCTCAGCTG TTGTACCACT 
CAGCTCTTTG GGAACACTTG GGCCTGGCAA CCTCCAGCAG CAAAGCTCTC CCAGATGTCA 
AAGTCTTCTA AAAATCAGAG CCAAAATGGT CCAGGAGAAA GAGGCAAACA AACGGCCTCT 
AGTGACTTGG CCCTCCACAC TCCCTCCCCA GAGGAGCTGG GTGAGAAGCC TTCAGCCGGG 
CCAAAGCCCA TCAGGAAGCC TTGTACCCCG AAGCAGCCAG GTCAGGCTGG CCCCCATGCA 
TCCTTCACAG TGTTTGATGA GGAACTTTCT AGCCAAGGCA AATCCGATTT AGCCAAGGCC 
CCGAGGGTGA GGTGCAGAGT CCAAACTCGT CTGAAGGTGA ACTTTAGCGA TGACGATGAA 
GATCGTCCGA CCCCTTCCCA TGGACTGGCT GAAAAACCCA AGAGACGAGG AGCTGCCCCT 
CGAGGCAGGG GGAGGGGGGG CAGGGCCTTG AGGCTGAAGA CGGATGCCCA AGGTGATCCA 
GGGGGCTCTG GGGACCAATC TGCCTGTATC AGGCGAACTC GCCAGAGCCA CAAAGCGGGG 
GTCGAGGAGC AAGTCCCTCA GAGGGCCCTT CGACGGCAGG CCAATGTAGA CGTGGAGCTC 
ATGAGGACCA TTCCTGAAGA ACAGACAACC ACGAAACTGG ACATGAGTGT GGAGATGCTG 
CAAGATTCCG ATGAGGGAGG CAGAGCCTCA GCAGGACAGA ATGCCTGGCA GGACACTGCC 
ATGGGAGAGT GTGAAGTGGT GAGACGAGAT GCCAGCAAGG AAGTGCCACC TTTCCCACCC 
CTAGAGAAGA GGGAAGGTGA TCGAAGTCAG CATGGTCCCC AGACCAGGCC TGCATCTACC 
CCGGCAGCCT TTGGCCAGCC CACCCTCGAA TCCATCTATG ACTCCCTGAG CATTGCCTTC 
CGCTGTGTCA GCCACTGCCC CCCGAGTGAG CTCTATGCTC AGCTGTGTCG TCTCCTTGCT 
CTGTGCCTGG GACACCGGGA CCCCCACACC ACTGCCTTGT TGGTCACTGA GTCTATGTCC 
ATCACCTCTC GGCACCAGCT GCTCACCACC GTGCACCGTC ATCTCCACAA AGCCCAGAAG 
CGGCAAGCAG CAGATGTGGT GGAGCAGCTC CAGGGCCTGA GCCTACAGGA AGAGCCCAGA 
GGGGTCCCCT TTGGCCGCCT GCAGCACCTC TTTTCATTCA GGACACCCAC CAGTGGCTGC 
TTCCCGCAGC CCGAGAAGGA GAGCTTCCAG GAGCTTCTGG CTCAGATGCC TAGCGGAGTG 
ACAGTGTGTG TGCTGGCTTT GGTCAGCCTC CAGTCTGGAA CTGTCAGGGA TACATTACTA 
TTGACACGGC TGGAAAAGAA CACTCCCCCC GTCACCATTC AGATACCCAC TGCCCAAACA 
AAGCTGCCTC TGAGTTCGGC CTTGGAAGAG TTTAATTTAA TCCAGAAAGA ACAGAAGGAG 
AATAGCAACT GTACAGAGAA GTCCAAGTGG TGGGCAACTC GGCTGAAACT GGATCGAAGG 
ATGGAGGTAT TTGGTTGGGG CCCTTCTTTC CACAGGGAAT TTCCATCATC TTCTCTTTAT 
GTGTCTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_007506531.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007506593.1 Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

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CloneID	OMc36356
Clone ID Related Accession (Same CDS sequence)	XM_007506532.2 , XM_007506531.2
Accession Version	XM_007506531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	separin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001584931.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007506531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT TTAAAGGAGC AGACTTTGGA AAATGGATGA GCAGCCAAGA GGAGGCCAAG 
GCTTTGCTGG TTGAGCTGAA GGAGTTCCTG TCCAGACCTC TGGATAGCCC CACGGGCCGT 
TCATTTGCAG CAGAACAAAG ACTTGCCTGT GATTCCATTT TGAGGGCTTG CAACCAACAG 
GTGGTGGCTG GGCTTGCTTG TCCTGACCAC TTGGGCAGCC TGTTGGAGCT GGCAGGGTTG 
GCCTGTGATG GCTACTTGAA AGCAACCCCT CATCGCACCC CACTCTACTT AGAACGCATC 
CTCTTCGTCT TCTTGCGAAA TATTTTTGCT CGGGTGGTCC CAAACGCTGT TCTCTACCTG 
ATCAAATCAT TGCATACCTG CCTAGTGCAG CATGTGCCAG TGGGTGCAAC CCAGGACTAT 
GAGGCTATTA TCCGAGGTGC CTTCTCTTTG CTTTGGAAAG GGGCAGAAGC ACTTAGAGAA 
CGTCAGCAAG CCTTCTCTGT TCGCCTGTGG GCCTTGAGTT TCTTGGTACT CTTGGAAGAT 
GAAAGTACCC CATGCAAATT CCCCCTTTTT TCTTCCCCAA CTGCTTGTCA AGCTGAAGCT 
GCCTGCCGGC TCTTTGAAGG CAGTGGGCAT CCTTTGGGTG AGGCAGATGC CCACTTCCTC 
AGTGAACAGC TTTCTACTTG TGTGATCAAA GTGCTGGTTG GGAAGGAAGC CAGTCCTTCA 
CATCCTCTCT CTCCCTGCAG GGCCCTCTGC CTCCTAGAGC TCACCTTAGA AAGGTGCCAA 
AGACTGTGCC TGAGCAGCTG CTGCACAGAG GCTGCTAGGG CTGTGCAGGA AGTCCAGGGT 
TATTTGGGGA ACACAGCCCT GGCCCCTAGC CTCCAGCTGT GCCTGTTGGG GGTGGAACTC 
CGAAGGCTGC TGGCTGGAGC CTTTGGGGGA GTGGGGGAAC TATTGCGCAA GGCCGAAGCT 
ATTCTCAGCT TTGGCGTCAA GGGCCCGTCA TCCTCATTTA GAGCTCTTGT GGAGACTTGT 
CAGTTCATCC TCTTGGTCCT AGAGAGTGAT ACTTTGAAAA AGTATGAATT GGATAGCATC 
CTGGGCCTCT CAGCCTTCTT GGGCAAATAC TGTCATCTTC TTCGCCAGCT TCGGGACAGC 
GCTCCTGTGG ATTGTCCCAA GCAGCAGCAA GTCTTGGCTC AGATGCATTT TCATGGACTC 
CAGCTCTTTA CCAGGATGGT TTATGATTTC TTCAGTGGTT CTAAGGTCAC AGATTTGACT 
GACTTGACCG AGCTTGTTGA GAGCTGTCAT TGTGCTGTTG TCTGGATGTT GGAGGCTCTA 
GAGGGCCTGA AGGACCAAGA GCAGGCAGAC TACCTTGGAA TCACTGCCTG CTGCACCAGT 
CACTTGGCCT ATAAGTTCTA CAACCAGAAG CTCTATGCTG AGGCCAGCAC CATCACTGAA 
CCTTTCTGTC AGAAGATTGG ACTGGCAACA TCTGGAACAT ACCCTGGGGT GCCTCCTGAG 
AAGCTGCACA AGTGCTTCCG GTTACATGTA GAGAGCCTGA AGAAGCTAGG CCAGCCAGCC 
CAGGGATGCA GGATGGTGGC TCTGTGGCTG AAGGCCCTGC AGCCCTGCAC CCTCGAGCAA 
ATGGCGGAGC CCATCAGTTT CTGGGTCCGT GTCAAGATGG ATGCCGCCAA GGCTGGTGAC 
AAGGAACTGC AGCTCAAGAC CCTTCGCGAT AGTCTGGCTG GTTGGGACAC AGAGCTGCTG 
ACACCCTTGC TGGTAGAAGA GTTGCGAAGC TATAAAGTGA TCCGGGCCGA CACAGGACAA 
GAACGCTTCA ATGTGATTTG TGACTTGTTG GAGCTGAGTA CAGAAGAGAC ACCAGCTGGG 
GCCTGGGCAC GGGCCACCTA TTTGATAGAG CTTGCCCAAG TGCTGTGCTA CTATGACTTT 
GCCAAGCATG CCAACTGCTC AGCTTTGGAG GCTGTGCAGG AGGCCCTGAA ACTGTTAGAG 
TCTGTACAGC CGGAAGGCCA GGCCACAGAC CAGTTTTTGG ATGATAAGGC ACAGGCTTTG 
CTGTGGCTTT ACATCTGTAG CCTGGAGACC AAAATGCAGA AAGGCATTGA ACAGGACTTG 
AGGCTGAAGT TGCAGCCCAG CTCGATAGAA TTGGAAACCA ATGACTTGAA CTATGAGGAT 
AAAATCCAGG ATGACCATTT CCTCTACAGT AGCATTGCCT TCAATCTGGC TGTGGATGCT 
GCTCAGTCTA AATGTTTAGA TCAGGCACTG GCCCTTTGGA AGGAGATGCT AAAAAAAGGG 
CAGGTGCCCA CCGTGAGATG TCTACAACAG ACCACTGCCT CCCTCCAGAT TCTGGCTGCT 
CTCTATCAGA TGGTGGCCAA GCCCCTACAG GCTCTGGAGA CACACTTGTT GCTCCGTACT 
CTCTCAGAAA ATGTTAAGGA CTACATTAGA GCAGCCAGTG CCTCCTGCCA TATCACTCGG 
CTCCTTCTGA CACTAGACTG TCCCGGCTAT GCTCAGTTCT ACTTGGAGGA GGCAGAGTCC 
AGTCTGCAGC TCAGTGATCA CACCAGTGAT GGATACTTGC TCCACACCCA AACCTGTGCC 
CTACTTCGAA GTCAGCTTTA CTGCCTCCAG AAAAAGGTGA CTGAAGGCCT CTCTTTGTTG 
CTGTCTGTGG TACGGGATTC AACCCTTCAA AAATCTTCTA AGGCTTGGTA CTTGCTTCGG 
GTCCAGGCTT TGCAGGTGGT GGCTGTCTAC CTTAGTCTTC CCTCGAGCAG TCTTTCTGTC 
CAGCAGTGGG AGCAACTTTA TACTCAAGGC TGGCAAACCT CTGAGATAGC TCTCATTGAG 
TCCCACAAGC TCCTTCGAAG CATTATCATC CTTCTGATGG GCAGAGACTT GCTCTTTGTT 
CAGCAGTCAG CAGTGGACGC TGCCTTTCTT GACTATGGTG AGAACCTAGT ACAGAAGTGG 
CAAGTTCTTG CAGAGGTCCT GGGATGCTCA GAGAAGCTGG TAACCTTGTT GGGACGCCTG 
GGCACTGTAA GTGAAGCCAA GGCCTTCTGC CTGGAGGCAC TGAGGCTTGC CATGAAGCTG 
CAGTGTGTTC GCCAGTGTTC CCTGTTCCTG GTGCTAAAGG GAGAACTAGA ACTAGCCCGA 
AATGATCTTG ACCTTTGCCA GTCGGACTTA GAGCAAGTGC TGTTTCTGCT TGGGTCTAGT 
ACAGAGTTCG AAGGGATAGC CCAGCTGCCA AACACTGTGA AAAGGATCCA TCCCCAGAAA 
GGACAACAGG AGCCTCGCAT ACAGCCCGGT TCTGAGCTAT CTGAAGAGGA AGCCTTCCTG 
AAGGGTCCTG CTCTGCAGCT GGTAGCCACT GTGGAGAAGG ATCCTGGCCC CTCAGTGTCC 
TCCCCTGTTC TGAAAAGCAA GCCTCGGCCT TGCCCTGCCT TCCTTTCCCA CTCGCTTTCC 
TGCAGCTGCA TTCTTTGTAC TGACCCTGCT CTGGTAGTTG TCTGCCTTCG GTGGCTGCTA 
GTCTCTGCCG GGGCAGAACT GTCCTCCGGT CATCAGGCCA AAGGCCTGGA CATGTTACGG 
TCTGTGCTCA GGAGATGCCC AGGGGCCACA CATCATCTGG CACAGGTTTT ACAAGCTACT 
TTGAGCCAAG AAGCATCCCC CTCACCTGCT CCAGGATTCT TAGAGGACCT CATTGCTCAG 
GCTTATGCTC ATTTAGCCCT AGAAGGCATA AACCATCCAC CGGACAAAGG CCTCTGGGAC 
ATCCTTGAGA CTGGCCTGAA TTTTGTTGCT TCCAGGACTC CTTTCCTGAA CCCCTGGCAT 
GCCAGCCTTC TTGTGACAAA GAGCCTGGCA ACACTATCCA GTCTCAGCTG TTGTACCACT 
CAGCTCTTTG GGAACACTTG GGCCTGGCAA CCTCCAGCAG CAAAGCTCTC CCAGATGTCA 
AAGTCTTCTA AAAATCAGAG CCAAAATGGT CCAGGAGAAA GAGGCAAACA AACGGCCTCT 
AGTGACTTGG CCCTCCACAC TCCCTCCCCA GAGGAGCTGG GTGAGAAGCC TTCAGCCGGG 
CCAAAGCCCA TCAGGAAGCC TTGTACCCCG AAGCAGCCAG GTCAGGCTGG CCCCCATGCA 
TCCTTCACAG TGTTTGATGA GGAACTTTCT AGCCAAGGCA AATCCGATTT AGCCAAGGCC 
CCGAGGGTGA GGTGCAGAGT CCAAACTCGT CTGAAGGTGA ACTTTAGCGA TGACGATGAA 
GATCGTCCGA CCCCTTCCCA TGGACTGGCT GAAAAACCCA AGAGACGAGG AGCTGCCCCT 
CGAGGCAGGG GGAGGGGGGG CAGGGCCTTG AGGCTGAAGA CGGATGCCCA AGGTGATCCA 
GGGGGCTCTG GGGACCAATC TGCCTGTATC AGGCGAACTC GCCAGAGCCA CAAAGCGGGG 
GTCGAGGAGC AAGTCCCTCA GAGGGCCCTT CGACGGCAGG CCAATGTAGA CGTGGAGCTC 
ATGAGGACCA TTCCTGAAGA ACAGACAACC ACGAAACTGG ACATGAGTGT GGAGATGCTG 
CAAGATTCCG ATGAGGGAGG CAGAGCCTCA GCAGGACAGA ATGCCTGGCA GGACACTGCC 
ATGGGAGAGT GTGAAGTGGT GAGACGAGAT GCCAGCAAGG AAGTGCCACC TTTCCCACCC 
CTAGAGAAGA GGGAAGGTGA TCGAAGTCAG CATGGTCCCC AGACCAGGCC TGCATCTACC 
CCGGCAGCCT TTGGCCAGCC CACCCTCGAA TCCATCTATG ACTCCCTGAG CATTGCCTTC 
CGCTGTGTCA GCCACTGCCC CCCGAGTGAG CTCTATGCTC AGCTGTGTCG TCTCCTTGCT 
CTGTGCCTGG GACACCGGGA CCCCCACACC ACTGCCTTGT TGGTCACTGA GTCTATGTCC 
ATCACCTCTC GGCACCAGCT GCTCACCACC GTGCACCGTC ATCTCCACAA AGCCCAGAAG 
CGGCAAGCAG CAGATGTGGT GGAGCAGCTC CAGGGCCTGA GCCTACAGGA AGAGCCCAGA 
GGGGTCCCCT TTGGCCGCCT GCAGCACCTC TTTTCATTCA GGACACCCAC CAGTGGCTGC 
TTCCCGCAGC CCGAGAAGGA GAGCTTCCAG GAGCTTCTGG CTCAGATGCC TAGCGGAGTG 
ACAGTGTGTG TGCTGGCTTT GGTCAGCCTC CAGTCTGGAA CTGTCAGGGA TACATTACTA 
TTGACACGGC TGGAAAAGAA CACTCCCCCC GTCACCATTC AGATACCCAC TGCCCAAACA 
AAGCTGCCTC TGAGTTCGGC CTTGGAAGAG TTTAATTTAA TCCAGAAAGA ACAGAAGGAG 
AATAGCAACT GTACAGAGAA GTCCAAGTGG TGGGCAACTC GGCTGAAACT GGATCGAAGG 
ATGGAGGTAT TTGGTTGGGG CCCTTCTTTC CACAGGGAAT TTCCATCATC TTCTCTTTAT 
GTGTCTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007506593.1
CDS	287..5698
Translation	MRNFKGADFGKWMSSQEEAKALLVELKEFLSRPLDSPTGRSFAAEQRLACDSILRACNQQVVAGLACPDHLGSLLELAGLACDGYLKATPHRTPLYLERILFVFLRNIFARVVPNAVLYLIKSLHTCLVQHVPVGATQDYEAIIRGAFSLLWKGAEALRERQQAFSVRLWALSFLVLLEDESTPCKFPLFSSPTACQAEAACRLFEGSGHPLGEADAHFLSEQLSTCVIKVLVGKEASPSHPLSPCRALCLLELTLERCQRLCLSSCCTEAARAVQEVQGYLGNTALAPSLQLCLLGVELRRLLAGAFGGVGELLRKAEAILSFGVKGPSSSFRALVETCQFILLVLESDTLKKYELDSILGLSAFLGKYCHLLRQLRDSAPVDCPKQQQVLAQMHFHGLQLFTRMVYDFFSGSKVTDLTDLTELVESCHCAVVWMLEALEGLKDQEQADYLGITACCTSHLAYKFYNQKLYAEASTITEPFCQKIGLATSGTYPGVPPEKLHKCFRLHVESLKKLGQPAQGCRMVALWLKALQPCTLEQMAEPISFWVRVKMDAAKAGDKELQLKTLRDSLAGWDTELLTPLLVEELRSYKVIRADTGQERFNVICDLLELSTEETPAGAWARATYLIELAQVLCYYDFAKHANCSALEAVQEALKLLESVQPEGQATDQFLDDKAQALLWLYICSLETKMQKGIEQDLRLKLQPSSIELETNDLNYEDKIQDDHFLYSSIAFNLAVDAAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASLQILAALYQMVAKPLQALETHLLLRTLSENVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGYLLHTQTCALLRSQLYCLQKKVTEGLSLLLSVVRDSTLQKSSKAWYLLRVQALQVVAVYLSLPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLRSIIILLMGRDLLFVQQSAVDAAFLDYGENLVQKWQVLAEVLGCSEKLVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQCSLFLVLKGELELARNDLDLCQSDLEQVLFLLGSSTEFEGIAQLPNTVKRIHPQKGQQEPRIQPGSELSEEEAFLKGPALQLVATVEKDPGPSVSSPVLKSKPRPCPAFLSHSLSCSCILCTDPALVVVCLRWLLVSAGAELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQATLSQEASPSPAPGFLEDLIAQAYAHLALEGINHPPDKGLWDILETGLNFVASRTPFLNPWHASLLVTKSLATLSSLSCCTTQLFGNTWAWQPPAAKLSQMSKSSKNQSQNGPGERGKQTASSDLALHTPSPEELGEKPSAGPKPIRKPCTPKQPGQAGPHASFTVFDEELSSQGKSDLAKAPRVRCRVQTRLKVNFSDDDEDRPTPSHGLAEKPKRRGAAPRGRGRGGRALRLKTDAQGDPGGSGDQSACIRRTRQSHKAGVEEQVPQRALRRQANVDVELMRTIPEEQTTTKLDMSVEMLQDSDEGGRASAGQNAWQDTAMGECEVVRRDASKEVPPFPPLEKREGDRSQHGPQTRPASTPAAFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLGHRDPHTTALLVTESMSITSRHQLLTTVHRHLHKAQKRQAADVVEQLQGLSLQEEPRGVPFGRLQHLFSFRTPTSGCFPQPEKESFQELLAQMPSGVTVCVLALVSLQSGTVRDTLLLTRLEKNTPPVTIQIPTAQTKLPLSSALEEFNLIQKEQKENSNCTEKSKWWATRLKLDRRMEVFGWGPSFHREFPSSSLYVSF

Target ORF information:

RefSeq Version	XM_007506531.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007506531.2

ORF Insert Sequence:

ATGAGGAACT TTAAAGGAGC AGACTTTGGA AAATGGATGA GCAGCCAAGA GGAGGCCAAG 
GCTTTGCTGG TTGAGCTGAA GGAGTTCCTG TCCAGACCTC TGGATAGCCC CACGGGCCGT 
TCATTTGCAG CAGAACAAAG ACTTGCCTGT GATTCCATTT TGAGGGCTTG CAACCAACAG 
GTGGTGGCTG GGCTTGCTTG TCCTGACCAC TTGGGCAGCC TGTTGGAGCT GGCAGGGTTG 
GCCTGTGATG GCTACTTGAA AGCAACCCCT CATCGCACCC CACTCTACTT AGAACGCATC 
CTCTTCGTCT TCTTGCGAAA TATTTTTGCT CGGGTGGTCC CAAACGCTGT TCTCTACCTG 
ATCAAATCAT TGCATACCTG CCTAGTGCAG CATGTGCCAG TGGGTGCAAC CCAGGACTAT 
GAGGCTATTA TCCGAGGTGC CTTCTCTTTG CTTTGGAAAG GGGCAGAAGC ACTTAGAGAA 
CGTCAGCAAG CCTTCTCTGT TCGCCTGTGG GCCTTGAGTT TCTTGGTACT CTTGGAAGAT 
GAAAGTACCC CATGCAAATT CCCCCTTTTT TCTTCCCCAA CTGCTTGTCA AGCTGAAGCT 
GCCTGCCGGC TCTTTGAAGG CAGTGGGCAT CCTTTGGGTG AGGCAGATGC CCACTTCCTC 
AGTGAACAGC TTTCTACTTG TGTGATCAAA GTGCTGGTTG GGAAGGAAGC CAGTCCTTCA 
CATCCTCTCT CTCCCTGCAG GGCCCTCTGC CTCCTAGAGC TCACCTTAGA AAGGTGCCAA 
AGACTGTGCC TGAGCAGCTG CTGCACAGAG GCTGCTAGGG CTGTGCAGGA AGTCCAGGGT 
TATTTGGGGA ACACAGCCCT GGCCCCTAGC CTCCAGCTGT GCCTGTTGGG GGTGGAACTC 
CGAAGGCTGC TGGCTGGAGC CTTTGGGGGA GTGGGGGAAC TATTGCGCAA GGCCGAAGCT 
ATTCTCAGCT TTGGCGTCAA GGGCCCGTCA TCCTCATTTA GAGCTCTTGT GGAGACTTGT 
CAGTTCATCC TCTTGGTCCT AGAGAGTGAT ACTTTGAAAA AGTATGAATT GGATAGCATC 
CTGGGCCTCT CAGCCTTCTT GGGCAAATAC TGTCATCTTC TTCGCCAGCT TCGGGACAGC 
GCTCCTGTGG ATTGTCCCAA GCAGCAGCAA GTCTTGGCTC AGATGCATTT TCATGGACTC 
CAGCTCTTTA CCAGGATGGT TTATGATTTC TTCAGTGGTT CTAAGGTCAC AGATTTGACT 
GACTTGACCG AGCTTGTTGA GAGCTGTCAT TGTGCTGTTG TCTGGATGTT GGAGGCTCTA 
GAGGGCCTGA AGGACCAAGA GCAGGCAGAC TACCTTGGAA TCACTGCCTG CTGCACCAGT 
CACTTGGCCT ATAAGTTCTA CAACCAGAAG CTCTATGCTG AGGCCAGCAC CATCACTGAA 
CCTTTCTGTC AGAAGATTGG ACTGGCAACA TCTGGAACAT ACCCTGGGGT GCCTCCTGAG 
AAGCTGCACA AGTGCTTCCG GTTACATGTA GAGAGCCTGA AGAAGCTAGG CCAGCCAGCC 
CAGGGATGCA GGATGGTGGC TCTGTGGCTG AAGGCCCTGC AGCCCTGCAC CCTCGAGCAA 
ATGGCGGAGC CCATCAGTTT CTGGGTCCGT GTCAAGATGG ATGCCGCCAA GGCTGGTGAC 
AAGGAACTGC AGCTCAAGAC CCTTCGCGAT AGTCTGGCTG GTTGGGACAC AGAGCTGCTG 
ACACCCTTGC TGGTAGAAGA GTTGCGAAGC TATAAAGTGA TCCGGGCCGA CACAGGACAA 
GAACGCTTCA ATGTGATTTG TGACTTGTTG GAGCTGAGTA CAGAAGAGAC ACCAGCTGGG 
GCCTGGGCAC GGGCCACCTA TTTGATAGAG CTTGCCCAAG TGCTGTGCTA CTATGACTTT 
GCCAAGCATG CCAACTGCTC AGCTTTGGAG GCTGTGCAGG AGGCCCTGAA ACTGTTAGAG 
TCTGTACAGC CGGAAGGCCA GGCCACAGAC CAGTTTTTGG ATGATAAGGC ACAGGCTTTG 
CTGTGGCTTT ACATCTGTAG CCTGGAGACC AAAATGCAGA AAGGCATTGA ACAGGACTTG 
AGGCTGAAGT TGCAGCCCAG CTCGATAGAA TTGGAAACCA ATGACTTGAA CTATGAGGAT 
AAAATCCAGG ATGACCATTT CCTCTACAGT AGCATTGCCT TCAATCTGGC TGTGGATGCT 
GCTCAGTCTA AATGTTTAGA TCAGGCACTG GCCCTTTGGA AGGAGATGCT AAAAAAAGGG 
CAGGTGCCCA CCGTGAGATG TCTACAACAG ACCACTGCCT CCCTCCAGAT TCTGGCTGCT 
CTCTATCAGA TGGTGGCCAA GCCCCTACAG GCTCTGGAGA CACACTTGTT GCTCCGTACT 
CTCTCAGAAA ATGTTAAGGA CTACATTAGA GCAGCCAGTG CCTCCTGCCA TATCACTCGG 
CTCCTTCTGA CACTAGACTG TCCCGGCTAT GCTCAGTTCT ACTTGGAGGA GGCAGAGTCC 
AGTCTGCAGC TCAGTGATCA CACCAGTGAT GGATACTTGC TCCACACCCA AACCTGTGCC 
CTACTTCGAA GTCAGCTTTA CTGCCTCCAG AAAAAGGTGA CTGAAGGCCT CTCTTTGTTG 
CTGTCTGTGG TACGGGATTC AACCCTTCAA AAATCTTCTA AGGCTTGGTA CTTGCTTCGG 
GTCCAGGCTT TGCAGGTGGT GGCTGTCTAC CTTAGTCTTC CCTCGAGCAG TCTTTCTGTC 
CAGCAGTGGG AGCAACTTTA TACTCAAGGC TGGCAAACCT CTGAGATAGC TCTCATTGAG 
TCCCACAAGC TCCTTCGAAG CATTATCATC CTTCTGATGG GCAGAGACTT GCTCTTTGTT 
CAGCAGTCAG CAGTGGACGC TGCCTTTCTT GACTATGGTG AGAACCTAGT ACAGAAGTGG 
CAAGTTCTTG CAGAGGTCCT GGGATGCTCA GAGAAGCTGG TAACCTTGTT GGGACGCCTG 
GGCACTGTAA GTGAAGCCAA GGCCTTCTGC CTGGAGGCAC TGAGGCTTGC CATGAAGCTG 
CAGTGTGTTC GCCAGTGTTC CCTGTTCCTG GTGCTAAAGG GAGAACTAGA ACTAGCCCGA 
AATGATCTTG ACCTTTGCCA GTCGGACTTA GAGCAAGTGC TGTTTCTGCT TGGGTCTAGT 
ACAGAGTTCG AAGGGATAGC CCAGCTGCCA AACACTGTGA AAAGGATCCA TCCCCAGAAA 
GGACAACAGG AGCCTCGCAT ACAGCCCGGT TCTGAGCTAT CTGAAGAGGA AGCCTTCCTG 
AAGGGTCCTG CTCTGCAGCT GGTAGCCACT GTGGAGAAGG ATCCTGGCCC CTCAGTGTCC 
TCCCCTGTTC TGAAAAGCAA GCCTCGGCCT TGCCCTGCCT TCCTTTCCCA CTCGCTTTCC 
TGCAGCTGCA TTCTTTGTAC TGACCCTGCT CTGGTAGTTG TCTGCCTTCG GTGGCTGCTA 
GTCTCTGCCG GGGCAGAACT GTCCTCCGGT CATCAGGCCA AAGGCCTGGA CATGTTACGG 
TCTGTGCTCA GGAGATGCCC AGGGGCCACA CATCATCTGG CACAGGTTTT ACAAGCTACT 
TTGAGCCAAG AAGCATCCCC CTCACCTGCT CCAGGATTCT TAGAGGACCT CATTGCTCAG 
GCTTATGCTC ATTTAGCCCT AGAAGGCATA AACCATCCAC CGGACAAAGG CCTCTGGGAC 
ATCCTTGAGA CTGGCCTGAA TTTTGTTGCT TCCAGGACTC CTTTCCTGAA CCCCTGGCAT 
GCCAGCCTTC TTGTGACAAA GAGCCTGGCA ACACTATCCA GTCTCAGCTG TTGTACCACT 
CAGCTCTTTG GGAACACTTG GGCCTGGCAA CCTCCAGCAG CAAAGCTCTC CCAGATGTCA 
AAGTCTTCTA AAAATCAGAG CCAAAATGGT CCAGGAGAAA GAGGCAAACA AACGGCCTCT 
AGTGACTTGG CCCTCCACAC TCCCTCCCCA GAGGAGCTGG GTGAGAAGCC TTCAGCCGGG 
CCAAAGCCCA TCAGGAAGCC TTGTACCCCG AAGCAGCCAG GTCAGGCTGG CCCCCATGCA 
TCCTTCACAG TGTTTGATGA GGAACTTTCT AGCCAAGGCA AATCCGATTT AGCCAAGGCC 
CCGAGGGTGA GGTGCAGAGT CCAAACTCGT CTGAAGGTGA ACTTTAGCGA TGACGATGAA 
GATCGTCCGA CCCCTTCCCA TGGACTGGCT GAAAAACCCA AGAGACGAGG AGCTGCCCCT 
CGAGGCAGGG GGAGGGGGGG CAGGGCCTTG AGGCTGAAGA CGGATGCCCA AGGTGATCCA 
GGGGGCTCTG GGGACCAATC TGCCTGTATC AGGCGAACTC GCCAGAGCCA CAAAGCGGGG 
GTCGAGGAGC AAGTCCCTCA GAGGGCCCTT CGACGGCAGG CCAATGTAGA CGTGGAGCTC 
ATGAGGACCA TTCCTGAAGA ACAGACAACC ACGAAACTGG ACATGAGTGT GGAGATGCTG 
CAAGATTCCG ATGAGGGAGG CAGAGCCTCA GCAGGACAGA ATGCCTGGCA GGACACTGCC 
ATGGGAGAGT GTGAAGTGGT GAGACGAGAT GCCAGCAAGG AAGTGCCACC TTTCCCACCC 
CTAGAGAAGA GGGAAGGTGA TCGAAGTCAG CATGGTCCCC AGACCAGGCC TGCATCTACC 
CCGGCAGCCT TTGGCCAGCC CACCCTCGAA TCCATCTATG ACTCCCTGAG CATTGCCTTC 
CGCTGTGTCA GCCACTGCCC CCCGAGTGAG CTCTATGCTC AGCTGTGTCG TCTCCTTGCT 
CTGTGCCTGG GACACCGGGA CCCCCACACC ACTGCCTTGT TGGTCACTGA GTCTATGTCC 
ATCACCTCTC GGCACCAGCT GCTCACCACC GTGCACCGTC ATCTCCACAA AGCCCAGAAG 
CGGCAAGCAG CAGATGTGGT GGAGCAGCTC CAGGGCCTGA GCCTACAGGA AGAGCCCAGA 
GGGGTCCCCT TTGGCCGCCT GCAGCACCTC TTTTCATTCA GGACACCCAC CAGTGGCTGC 
TTCCCGCAGC CCGAGAAGGA GAGCTTCCAG GAGCTTCTGG CTCAGATGCC TAGCGGAGTG 
ACAGTGTGTG TGCTGGCTTT GGTCAGCCTC CAGTCTGGAA CTGTCAGGGA TACATTACTA 
TTGACACGGC TGGAAAAGAA CACTCCCCCC GTCACCATTC AGATACCCAC TGCCCAAACA 
AAGCTGCCTC TGAGTTCGGC CTTGGAAGAG TTTAATTTAA TCCAGAAAGA ACAGAAGGAG 
AATAGCAACT GTACAGAGAA GTCCAAGTGG TGGGCAACTC GGCTGAAACT GGATCGAAGG 
ATGGAGGTAT TTGGTTGGGG CCCTTCTTTC CACAGGGAAT TTCCATCATC TTCTCTTTAT 
GTGTCTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ESPL1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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