CAD cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAD
Entrez Gene ID	100026061
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001371087.3	XP_001371124.1	CAD protein isoform X2
XM_007475999.2	XP_007476061.1	CAD protein isoform X1

Pathways from BioSystems
Gene Ontology

The following CAD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMc06126	XM_007475999.2 Latest version!	Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc06127	XM_001371087.3 Latest version!	Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAD ( XM_007475999.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007476061.1 Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

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CloneID	OMc06126
Clone ID Related Accession (Same CDS sequence)	XM_007475999.2
Accession Version	XM_007475999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007475999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TGGTGTTAGA GGATGGGTCG GTCCTGCGGG GCCAGTCTTT CGGCGCGGCC 
GTATGCGCCG CCGGGGAAGT AGTGTTCCAG ACTGGCATGG TGGGCTACCC CGAGGCCCTC 
ACCGATCCCT CCTACAAAGC CCAGATCTTG GTCCTGACGT ATCCGCTCGT GGGCAACTAT 
GGCATCCCCC CAGATGAGGA AGACGAGTTC AACCTCAGCC AGTGGTTTGA GTCATCAGGG 
ATTCATGTGG CAGGACTTGT GGTGGGAGAA TGCTGCTCTA CACCTAGCCA CTGGAGCGCC 
ACCCGAACCT TGCACCAGTG GCTGGAGGAG CATGGTATAC CTGGTCTCCA AGGAGTTGAT 
ACTAGGGAAC TGACAAAGAA GTTGCGGGAA CAAGGATCAC TTCTGGGAAA GCTGGTGCAG 
GATGGGATAG AGCCTTCATC CCTACCATTT TTGGATCCCA ATGCTCGAGC TCTTGTACCT 
GAGGTTTCCA TTAAGGAACC AAGGATATAC AATGCAAGGG GGTCTCCACG AATTCTTGCT 
TTGGATTGTG GGCTCAAATA CAGTCAGATT AGATGCTTGT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTGC CCTGGGACTA CAAATTAGAC AGCCATGAGT ATGAAGGTCT GTTCCTAAGT 
AATGGCCCTG GAGACCCTGC TTCTTGCCCT AATGTGGTAT CTATGCTGAG CAATGTCCTT 
TCTGAGCCAG ACCCCCGACC TGTCTTTGGT ATCTGCCTGG GTCACCAGCT ATTGGCCTTA 
GCCATCGGAG CCAAGACTTA CAAAATGAGG TATGGCAACC GTGGCCACAA CCAGCCATGC 
CTGCTAAAGG GCTCTGGACG GTGTTTCTTA ACATCTCAGA ATCATGGCTT TGCAGTGGAA 
ACAGAATCTC TGCCACCAGG CTGGACCCCT CTTTTCACAA ATGCCAATGA TCTTTCCAAT 
GAAGGCATTG TACATGAGAC CCTGCCCTTC TTCAGCGTCC AGTTCCACCC TGAGCACCGA 
GCCGGCCCCT CTGATATGGA GTTGCTTTTT GACATCTTCC TGGAGACCGT GAAGGAGAAC 
AGGGCTGGGG GCAAGACAGT TCGTGAAAGG TTAATGGAGC GTCTCTGTCC CTCGGGTACC 
CCTACCCCAA GAGACATGCC TCCATCTCCC CGAAAAGTGC TGATCCTGGG CTCAGGGGGT 
CTCTCCATTG GCCAGGCTGG AGAGTTTGAC TACTCTGGCT CTCAGGCAAT CAAGGCCCTA 
AAGGAGGAAA ATATTCAAAC GCTGCTGATA AATCCTAATA TTGCCACAGT GCAAACCTCC 
CAGGGACTGG CGGACAAAGT CTATTTCTTG CCTATAACTC CTCATTATGT GACTCAGGTT 
ATTCGTAATG AGCGTCCAGA TGGTGTATTA CTGACATTTG GGGGCCAGAC AGCACTGAAT 
TGTGGTGTGG AGCTGACTAA GGCTGGGGTT CTGGCCCGGT ATGGAGTACG GGTCCTAGGC 
ACCCCCGTAG AGACAATTGA GCTGACTGAG GATCGGCGTG CTTTTGCAGC AAGGATGGCA 
GAAATTGGAG AACATGTGGC CCCGAGCGAG GCTGCAAACT CCCTGGAACA GGCGCAAGCT 
GCTGCAGAAC GACTGGGGTA CCCGGTCCTG GTACGTGCTG CCTTTGCCCT AGGTGGCCTT 
GGCTCCGGCT TTGCTTCTAG CAGAGAAGAA CTCTCCTCTC TGGTGGCCCC AGCCTTTGCT 
CACACTAGTC AAGTACTGGT GGACAAGTCC CTCAAGGGAT GGAAAGAAAT AGAATATGAA 
GTGGTGAGAG ATGCCTATGG CAACTGTGTC ACGGTATGTA ACATGGAAAA TCTAGATCCA 
CTGGGCATTC ACACTGGTGA ATCTATAGTT GTGGCCCCAA GCCAGACACT AAACGACAGG 
GAATATCAGC TGTTGCGACA GACTGCTATC AAGGTGACTC AACACCTGGG CATTGTTGGG 
GAGTGCAATG TACAATATGC CTTGAACCCT GAGTCTGAAC AGTACTATAT CATTGAAGTG 
AATGCACGAC TGTCTCGGAG TTCGGCCCTT GCCAGTAAAG CCACTGGTTA TCCTCTGGCC 
TATGTAGCAG CCAAGCTGGC TTTGGGTATC TCCTTGCCTG ATCTCAGGAA CTCGGTAACT 
GGGGGTACTG CTGCTTTTGA GCCCAGCCTG GATTACTGCG TGGTTAAGGT CCCTCGCTGG 
GACCTTAGCA AGTTCCTCCG AGTTAGCACA AGGATTGGAA GCTGCATGAA GAGTGTTGGT 
GAGGTCATGG CTATTGGACG TGGGTTCGAA GAGGCATTCC AAAAGGCCTT GCGCATGGTG 
GATGAGAACT GTGTTGGCTT TGATCACACA ATGAAGCCCG TCAGTGACAT GGAGCTGGAA 
ACGCCAACAG ACAAGCGGAT CTTTGTGGTA GCAGCTGCTT TGTGGGCAGG ATATACCATA 
GACCGACTGT ATGAGCTTAC TCGCATTGAT CGCTGGTTCC TGCAGTGCAT GAAACGCATT 
ATAGAGCACA CTCGAAAACT AGAGGAACAC CGTGGGCAGC CCTTGCCCCC TGATTTGCTG 
CACCAGGCCA AGCGCCTTGG ATTCTCCGAC AAGCAGATTG CCCTCGCAGT ACTCAGCACA 
GAGCTGGCCG TACGAAAACT ACGGCAGGAG CTGGGCATCT GTCCAGCGGT CAAGCAGATT 
GATACAGTGG CAGCAGAGTG GCCAGCTCAA ACCAACTATC TGTACCTGAC TTATGGTGGC 
ACCACCCATG ACCTGGCTTT CCACGCTCCC CATGTCCTGG TCTTGGGCTC TGGCGTTTAC 
CGCATTGGCT CCAGTGTGGA ATTTGACTGG TGTGCTGTGG GCTGCATCCG GCAGCTGCGC 
AAGATGGGGT ATAAGACTAT TATGGTGAAC TATAACCCTG AGACAGTCAG TACTGATTAT 
GACATGTGTG ATCGGCTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGATATCTAT 
GAGCTAGAAA ACCCTGAGGG TGTAATCCTC TCCATGGGAG GTCAGTTGCC CAACAACATG 
GCAATGGCCC TGCATAGGCA GCAGTGCCGA GTCCTTGGCA CATCCCCAGA AGCCATCGAC 
TCTGCTGAGA ACAGATTCAA ATTTTCCCGA CTTCTTGACA CCATTGGCAT CAGTCAGCCT 
CAGTGGCGAG AACTCAGTGA CTTGGAGTCT GCTCGCCAGT TCTGTCACAC CGTGGGGTAC 
CCTTGTGTTG TCCGCCCATC CTATGTGCTG AGTGGTGCAG CTATGAATGT GGCCTATACT 
GATGGAGACC TTGAGCGATT TCTGAGCAGT GCAGCTGCTG TCTCCAAAGA ACATCCTGTG 
GTCATCTCCA AGTTCATCCA AGAGGCCAAG GAAATTGACG TGGATGCTGT GGCTTGTGAT 
GGCATGGTGG TTGCTGTAGC TATCTCAGAG CATGTGGAGA ATGCAGGGGT GCACTCAGGG 
GATGCCACTT TGGTGACTCC TCCTCAAGAC ATCACACCTA AGACATTGGA AAGGATCAAA 
GCAATTGTTC ACGCTGTGGG TCAAGAGCTA CAGGTCACAG GGCCTTTCAA TCTTCAGCTC 
ATTGCCAAGG ATGACCAGCT CAAGGTTATC GAGTGCAATG TCCGTGTCTC TCGGTCCTTC 
CCCTTTGTCT CTAAGACACT TGGAGTAGAT CTGGTAGCCC TGGCTACCCG AATTATCATG 
GGTGAGGAGG TGGAGCCTGT TGGGCTCATG GCTGGAACTG GAGTAGTGGG AGTTAAGGTG 
CCCCAGTTCT CATTCTCACG CCTGGCAGGA GCAGATGTGG TGCTGGGTGT AGAGATGACC 
AGCACTGGGG AAGTGGCTGG CTTTGGGGAG AGCCGCTGTG AGGCCTACCT CAAGGCTATG 
CTAAGCACTG GCTTCAAGAT CCCCAAGAAG AATATCCTGC TGACTATTGG TAGTTACAAG 
AACAAGAGTG AGCTGCTCCC TACAGTGAGG CTGTTGGAGA GTCTAGGCTA TAGCTTATAT 
GCCAGTTTGG GCACAGCAGA CTTCTACACA GAGCATGGTG TCAAGGTTAC AGCCGTGGAC 
TGGCACTTTG AGGAGGCAGT TGAAGGTGAA TGTCCTCCAC AACGAAGCAT CCTGGAACAG 
TTGGCAGAAA ACCACTTTGA ACTGGTCATC AACCTGTCAA TGCGAGGGGC AGGGGGTCGA 
CGCCTCTCCT CCTTTGTAAC CAAGGGGTAC CGGACCCGTC GCCTGGCTGC AGACTTCTCT 
GTGCCACTCA TCATTGACAT CAAGTGCACT AAGCTATTTG TAGAGGCATT GGGCCAGATT 
GGGCCAGCCC CTCCCCTGAA GGTGCATGTT GACTGTATGA CCTCACAGAA GCTTGTGCGT 
CTGCCAGGGC TGATTGATGT ACATGTGCAC CTACGGGAAC CAGGTGGTAC TCACAAGGAG 
GACTTTGCCT CAGGCACAGC GGCTGCCCTA GCTGGTGGGG TCACCATGGT GTGTGCTATG 
CCCAACACTC GACCTCCCAT CATAGATGCC CCAGCCCTTG CCCTTGCACA GAAGTTGGCA 
GAAACGGGGG CCCGCTGTGA CTATGCCTTG TTCCTTGGGG CCTCGTCAGA GAATGCAGGA 
ACCCTGGGTG CAGTGGCTGG GGCAGCTGCA GGATTGAAGC TCTATCTCAA TGAGACATTC 
TCTGAACTTC AACTGGAGAG CATCTCTCAT TGGATGGAGC ACTTTGAGAC ATGGCCACAC 
CATCTCCCCA TTGTGGCTCA TGCAGAACGA CAGACCGTAG CTGCCATCCT TATGGTAGCC 
CAGTTGGCTC AGCGACCTGT CCACATCTGC CACGTGGCCC GGAAGGAGGA GATCCTATTA 
ATTAAAGCTG CAAAGGCACG GGGGGTATCA GTGACCTGCG AGGTGGCCCC CCACCATCTC 
TTCCTCAGCC AAGCAGACCT TGAGCGCCTG GGGCCTGGGA AAGGTGAAGT CCGGCCTGAG 
CTCGGCACCC TTGAGGACGT GGAGGCTCTC TGGGAGAACA TGGCCATCAT TGATTGCTTT 
GCCTCAGACC ATGCTCCTCA CACCCTGGAA GAAAAATCTG GGTCCAAGCC TCCCCCTGGC 
TTCCCAGGTC TGGAGACAAT GCTTCCATTG CTGCTAACAG CGGTGAGCGA GGGCCGACTC 
AGCCTAGATG ACCTGCTGCA GAAGCTGCAC CACAATCCCC GACGGATCTT TCACTTGCCC 
CAGCAGGAGG ACACCTATGT TGAGGTGGAT CTGGAGCATG AATGGATCAT CCCCAGCCAC 
ATGCCCTTCT CCAAGGCACA TTGGACACCT TTTGAGGGAC AGCGAGTGAA GGGCACAATA 
CGGCGAGTTG TCCTTCGAGG GGAGGTTGCC TACATTGATG GTCAGGTACT AGTGCCCCCT 
GGCTACGGGC AAGATGTGAG GAAGTGGCCT CAAGGCCCAG TGCCTCAGCA CCTGCCTTCA 
GCCCCACCCA GTGAAAGTAT CAAGACTCCA GAAAGACCCC GACGTGGCCT CCCAGGCCAT 
CCTGATGGGC GATTCCATCT CCCACCTCGA ATCCACCGAG CTTCTGACCC TGGCCTTCCA 
GCTGTGTTCA TCGGCCCAAG AGCCAGGACC CCACAAGGCA ACAGAGTGTG GGCTGAAGAA 
CCTAAGGAGA AACCCTCCCG CAAGACAGTG GAGCCAGATC TCGCAGGAAC TCCTGATAGC 
TGCTGCTACC CTCCTCCAGT GCCTAGGCAG GCCTCACCCC AGAACTTGGG TACTCCTAGC 
CTGTTGCATA CTCAGACTTC ACCCTTGCTG CATTCTCTAG TTGGCCAGCA TATACTGTCT 
GTTCGTCAGT TTACCAAAGA TCAGATGTCC CACCTGTTCA ATGTGGCACA CACCCTGCGG 
ATGATGGTCC AGAAGGAACG GAGCATAGAC ATTCTCAAGG GCAAGGTGAT GGCCTCCATG 
TTTTATGAAG TGAGCACTCG TACCAGCAGT TCCTTTGCAG CAGCTATGGC CCGGCTCGGA 
GGCTCTGTGC TCAACTTCTC TGAAGCTACA TCGTCTGTCC AGAAGGGAGA ATCCCTAGCA 
GATTCTGTCC AGACCATGAG CTGCTATGCC GATGTTGTTG TGCTCCGGCA CCCCCAACCT 
GGAGCTGTGG AGCTGGCAGC CAAGCACTGC CGGAGGCCAG TGATCAATGC TGGGGATGGA 
GTAGGAGAGC ACCCAACCCA GGCCTTGCTG GATGTCTTTA CCATTCGAGA GGAGCTAGGA 
ACTGTCAATG GCATGACGAT CACCATGGTA GGAGATCTCA AGCACGGACG GACAGTACAC 
TCCCTTGCCT GCCTGCTCAC ACAGTATCGG GTCAGTCTTC GCTATGTGGC ACCCCCCAGC 
CTGCGCATGC CACCAGATGT CTTGTCTTTT GTAGCTTCCA GAGGGACTAA GCAGGAGGAA 
TTTGAGAGCA TTGAGGAGGC CCTACCTGAC ACAGATGTCC TTTATATGAC TCGGATTCAA 
AAGGAACGGT TTGGATCCAC CCAAGAGTAT GAAGCTTGCT TTGGCCAATT CATCCTCACA 
CCCCACATCA TGACCCGGGC CAAGAAGAAG ATGGTCGTGA TGCACCCAAT GCCTCGGGTC 
AACGAGATCA GTGTGGAGGT AGACTCTGAC CCCCGAGCTG CCTACTTCCG GCAGGCTGAG 
AACGGCATGT ACATGCGGAT GGCCCTCCTC GCTACTGTAC TGGGTCGCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007476061.1
CDS	197..6910
Translation	MAALVLEDGSVLRGQSFGAAVCAAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLVGNYGIPPDEEDEFNLSQWFESSGIHVAGLVVGECCSTPSHWSATRTLHQWLEEHGIPGLQGVDTRELTKKLREQGSLLGKLVQDGIEPSSLPFLDPNARALVPEVSIKEPRIYNARGSPRILALDCGLKYSQIRCLCQRGAEVTVVPWDYKLDSHEYEGLFLSNGPGDPASCPNVVSMLSNVLSEPDPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLKGSGRCFLTSQNHGFAVETESLPPGWTPLFTNANDLSNEGIVHETLPFFSVQFHPEHRAGPSDMELLFDIFLETVKENRAGGKTVRERLMERLCPSGTPTPRDMPPSPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASSREELSSLVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGISLPDLRNSVTGGTAAFEPSLDYCVVKVPRWDLSKFLRVSTRIGSCMKSVGEVMAIGRGFEEAFQKALRMVDENCVGFDHTMKPVSDMELETPTDKRIFVVAAALWAGYTIDRLYELTRIDRWFLQCMKRIIEHTRKLEEHRGQPLPPDLLHQAKRLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYGGTTHDLAFHAPHVLVLGSGVYRIGSSVEFDWCAVGCIRQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGMVVAVAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEEVEPVGLMAGTGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVEGECPPQRSILEQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAETGARCDYALFLGASSENAGTLGAVAGAAAGLKLYLNETFSELQLESISHWMEHFETWPHHLPIVAHAERQTVAAILMVAQLAQRPVHICHVARKEEILLIKAAKARGVSVTCEVAPHHLFLSQADLERLGPGKGEVRPELGTLEDVEALWENMAIIDCFASDHAPHTLEEKSGSKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQKLHHNPRRIFHLPQQEDTYVEVDLEHEWIIPSHMPFSKAHWTPFEGQRVKGTIRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGPVPQHLPSAPPSESIKTPERPRRGLPGHPDGRFHLPPRIHRASDPGLPAVFIGPRARTPQGNRVWAEEPKEKPSRKTVEPDLAGTPDSCCYPPPVPRQASPQNLGTPSLLHTQTSPLLHSLVGQHILSVRQFTKDQMSHLFNVAHTLRMMVQKERSIDILKGKVMASMFYEVSTRTSSSFAAAMARLGGSVLNFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDVFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPDVLSFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYMRMALLATVLGRY

Target ORF information:

RefSeq Version	XM_007475999.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007475999.2

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTAGA GGATGGGTCG GTCCTGCGGG GCCAGTCTTT CGGCGCGGCC 
GTATGCGCCG CCGGGGAAGT AGTGTTCCAG ACTGGCATGG TGGGCTACCC CGAGGCCCTC 
ACCGATCCCT CCTACAAAGC CCAGATCTTG GTCCTGACGT ATCCGCTCGT GGGCAACTAT 
GGCATCCCCC CAGATGAGGA AGACGAGTTC AACCTCAGCC AGTGGTTTGA GTCATCAGGG 
ATTCATGTGG CAGGACTTGT GGTGGGAGAA TGCTGCTCTA CACCTAGCCA CTGGAGCGCC 
ACCCGAACCT TGCACCAGTG GCTGGAGGAG CATGGTATAC CTGGTCTCCA AGGAGTTGAT 
ACTAGGGAAC TGACAAAGAA GTTGCGGGAA CAAGGATCAC TTCTGGGAAA GCTGGTGCAG 
GATGGGATAG AGCCTTCATC CCTACCATTT TTGGATCCCA ATGCTCGAGC TCTTGTACCT 
GAGGTTTCCA TTAAGGAACC AAGGATATAC AATGCAAGGG GGTCTCCACG AATTCTTGCT 
TTGGATTGTG GGCTCAAATA CAGTCAGATT AGATGCTTGT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTGC CCTGGGACTA CAAATTAGAC AGCCATGAGT ATGAAGGTCT GTTCCTAAGT 
AATGGCCCTG GAGACCCTGC TTCTTGCCCT AATGTGGTAT CTATGCTGAG CAATGTCCTT 
TCTGAGCCAG ACCCCCGACC TGTCTTTGGT ATCTGCCTGG GTCACCAGCT ATTGGCCTTA 
GCCATCGGAG CCAAGACTTA CAAAATGAGG TATGGCAACC GTGGCCACAA CCAGCCATGC 
CTGCTAAAGG GCTCTGGACG GTGTTTCTTA ACATCTCAGA ATCATGGCTT TGCAGTGGAA 
ACAGAATCTC TGCCACCAGG CTGGACCCCT CTTTTCACAA ATGCCAATGA TCTTTCCAAT 
GAAGGCATTG TACATGAGAC CCTGCCCTTC TTCAGCGTCC AGTTCCACCC TGAGCACCGA 
GCCGGCCCCT CTGATATGGA GTTGCTTTTT GACATCTTCC TGGAGACCGT GAAGGAGAAC 
AGGGCTGGGG GCAAGACAGT TCGTGAAAGG TTAATGGAGC GTCTCTGTCC CTCGGGTACC 
CCTACCCCAA GAGACATGCC TCCATCTCCC CGAAAAGTGC TGATCCTGGG CTCAGGGGGT 
CTCTCCATTG GCCAGGCTGG AGAGTTTGAC TACTCTGGCT CTCAGGCAAT CAAGGCCCTA 
AAGGAGGAAA ATATTCAAAC GCTGCTGATA AATCCTAATA TTGCCACAGT GCAAACCTCC 
CAGGGACTGG CGGACAAAGT CTATTTCTTG CCTATAACTC CTCATTATGT GACTCAGGTT 
ATTCGTAATG AGCGTCCAGA TGGTGTATTA CTGACATTTG GGGGCCAGAC AGCACTGAAT 
TGTGGTGTGG AGCTGACTAA GGCTGGGGTT CTGGCCCGGT ATGGAGTACG GGTCCTAGGC 
ACCCCCGTAG AGACAATTGA GCTGACTGAG GATCGGCGTG CTTTTGCAGC AAGGATGGCA 
GAAATTGGAG AACATGTGGC CCCGAGCGAG GCTGCAAACT CCCTGGAACA GGCGCAAGCT 
GCTGCAGAAC GACTGGGGTA CCCGGTCCTG GTACGTGCTG CCTTTGCCCT AGGTGGCCTT 
GGCTCCGGCT TTGCTTCTAG CAGAGAAGAA CTCTCCTCTC TGGTGGCCCC AGCCTTTGCT 
CACACTAGTC AAGTACTGGT GGACAAGTCC CTCAAGGGAT GGAAAGAAAT AGAATATGAA 
GTGGTGAGAG ATGCCTATGG CAACTGTGTC ACGGTATGTA ACATGGAAAA TCTAGATCCA 
CTGGGCATTC ACACTGGTGA ATCTATAGTT GTGGCCCCAA GCCAGACACT AAACGACAGG 
GAATATCAGC TGTTGCGACA GACTGCTATC AAGGTGACTC AACACCTGGG CATTGTTGGG 
GAGTGCAATG TACAATATGC CTTGAACCCT GAGTCTGAAC AGTACTATAT CATTGAAGTG 
AATGCACGAC TGTCTCGGAG TTCGGCCCTT GCCAGTAAAG CCACTGGTTA TCCTCTGGCC 
TATGTAGCAG CCAAGCTGGC TTTGGGTATC TCCTTGCCTG ATCTCAGGAA CTCGGTAACT 
GGGGGTACTG CTGCTTTTGA GCCCAGCCTG GATTACTGCG TGGTTAAGGT CCCTCGCTGG 
GACCTTAGCA AGTTCCTCCG AGTTAGCACA AGGATTGGAA GCTGCATGAA GAGTGTTGGT 
GAGGTCATGG CTATTGGACG TGGGTTCGAA GAGGCATTCC AAAAGGCCTT GCGCATGGTG 
GATGAGAACT GTGTTGGCTT TGATCACACA ATGAAGCCCG TCAGTGACAT GGAGCTGGAA 
ACGCCAACAG ACAAGCGGAT CTTTGTGGTA GCAGCTGCTT TGTGGGCAGG ATATACCATA 
GACCGACTGT ATGAGCTTAC TCGCATTGAT CGCTGGTTCC TGCAGTGCAT GAAACGCATT 
ATAGAGCACA CTCGAAAACT AGAGGAACAC CGTGGGCAGC CCTTGCCCCC TGATTTGCTG 
CACCAGGCCA AGCGCCTTGG ATTCTCCGAC AAGCAGATTG CCCTCGCAGT ACTCAGCACA 
GAGCTGGCCG TACGAAAACT ACGGCAGGAG CTGGGCATCT GTCCAGCGGT CAAGCAGATT 
GATACAGTGG CAGCAGAGTG GCCAGCTCAA ACCAACTATC TGTACCTGAC TTATGGTGGC 
ACCACCCATG ACCTGGCTTT CCACGCTCCC CATGTCCTGG TCTTGGGCTC TGGCGTTTAC 
CGCATTGGCT CCAGTGTGGA ATTTGACTGG TGTGCTGTGG GCTGCATCCG GCAGCTGCGC 
AAGATGGGGT ATAAGACTAT TATGGTGAAC TATAACCCTG AGACAGTCAG TACTGATTAT 
GACATGTGTG ATCGGCTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGATATCTAT 
GAGCTAGAAA ACCCTGAGGG TGTAATCCTC TCCATGGGAG GTCAGTTGCC CAACAACATG 
GCAATGGCCC TGCATAGGCA GCAGTGCCGA GTCCTTGGCA CATCCCCAGA AGCCATCGAC 
TCTGCTGAGA ACAGATTCAA ATTTTCCCGA CTTCTTGACA CCATTGGCAT CAGTCAGCCT 
CAGTGGCGAG AACTCAGTGA CTTGGAGTCT GCTCGCCAGT TCTGTCACAC CGTGGGGTAC 
CCTTGTGTTG TCCGCCCATC CTATGTGCTG AGTGGTGCAG CTATGAATGT GGCCTATACT 
GATGGAGACC TTGAGCGATT TCTGAGCAGT GCAGCTGCTG TCTCCAAAGA ACATCCTGTG 
GTCATCTCCA AGTTCATCCA AGAGGCCAAG GAAATTGACG TGGATGCTGT GGCTTGTGAT 
GGCATGGTGG TTGCTGTAGC TATCTCAGAG CATGTGGAGA ATGCAGGGGT GCACTCAGGG 
GATGCCACTT TGGTGACTCC TCCTCAAGAC ATCACACCTA AGACATTGGA AAGGATCAAA 
GCAATTGTTC ACGCTGTGGG TCAAGAGCTA CAGGTCACAG GGCCTTTCAA TCTTCAGCTC 
ATTGCCAAGG ATGACCAGCT CAAGGTTATC GAGTGCAATG TCCGTGTCTC TCGGTCCTTC 
CCCTTTGTCT CTAAGACACT TGGAGTAGAT CTGGTAGCCC TGGCTACCCG AATTATCATG 
GGTGAGGAGG TGGAGCCTGT TGGGCTCATG GCTGGAACTG GAGTAGTGGG AGTTAAGGTG 
CCCCAGTTCT CATTCTCACG CCTGGCAGGA GCAGATGTGG TGCTGGGTGT AGAGATGACC 
AGCACTGGGG AAGTGGCTGG CTTTGGGGAG AGCCGCTGTG AGGCCTACCT CAAGGCTATG 
CTAAGCACTG GCTTCAAGAT CCCCAAGAAG AATATCCTGC TGACTATTGG TAGTTACAAG 
AACAAGAGTG AGCTGCTCCC TACAGTGAGG CTGTTGGAGA GTCTAGGCTA TAGCTTATAT 
GCCAGTTTGG GCACAGCAGA CTTCTACACA GAGCATGGTG TCAAGGTTAC AGCCGTGGAC 
TGGCACTTTG AGGAGGCAGT TGAAGGTGAA TGTCCTCCAC AACGAAGCAT CCTGGAACAG 
TTGGCAGAAA ACCACTTTGA ACTGGTCATC AACCTGTCAA TGCGAGGGGC AGGGGGTCGA 
CGCCTCTCCT CCTTTGTAAC CAAGGGGTAC CGGACCCGTC GCCTGGCTGC AGACTTCTCT 
GTGCCACTCA TCATTGACAT CAAGTGCACT AAGCTATTTG TAGAGGCATT GGGCCAGATT 
GGGCCAGCCC CTCCCCTGAA GGTGCATGTT GACTGTATGA CCTCACAGAA GCTTGTGCGT 
CTGCCAGGGC TGATTGATGT ACATGTGCAC CTACGGGAAC CAGGTGGTAC TCACAAGGAG 
GACTTTGCCT CAGGCACAGC GGCTGCCCTA GCTGGTGGGG TCACCATGGT GTGTGCTATG 
CCCAACACTC GACCTCCCAT CATAGATGCC CCAGCCCTTG CCCTTGCACA GAAGTTGGCA 
GAAACGGGGG CCCGCTGTGA CTATGCCTTG TTCCTTGGGG CCTCGTCAGA GAATGCAGGA 
ACCCTGGGTG CAGTGGCTGG GGCAGCTGCA GGATTGAAGC TCTATCTCAA TGAGACATTC 
TCTGAACTTC AACTGGAGAG CATCTCTCAT TGGATGGAGC ACTTTGAGAC ATGGCCACAC 
CATCTCCCCA TTGTGGCTCA TGCAGAACGA CAGACCGTAG CTGCCATCCT TATGGTAGCC 
CAGTTGGCTC AGCGACCTGT CCACATCTGC CACGTGGCCC GGAAGGAGGA GATCCTATTA 
ATTAAAGCTG CAAAGGCACG GGGGGTATCA GTGACCTGCG AGGTGGCCCC CCACCATCTC 
TTCCTCAGCC AAGCAGACCT TGAGCGCCTG GGGCCTGGGA AAGGTGAAGT CCGGCCTGAG 
CTCGGCACCC TTGAGGACGT GGAGGCTCTC TGGGAGAACA TGGCCATCAT TGATTGCTTT 
GCCTCAGACC ATGCTCCTCA CACCCTGGAA GAAAAATCTG GGTCCAAGCC TCCCCCTGGC 
TTCCCAGGTC TGGAGACAAT GCTTCCATTG CTGCTAACAG CGGTGAGCGA GGGCCGACTC 
AGCCTAGATG ACCTGCTGCA GAAGCTGCAC CACAATCCCC GACGGATCTT TCACTTGCCC 
CAGCAGGAGG ACACCTATGT TGAGGTGGAT CTGGAGCATG AATGGATCAT CCCCAGCCAC 
ATGCCCTTCT CCAAGGCACA TTGGACACCT TTTGAGGGAC AGCGAGTGAA GGGCACAATA 
CGGCGAGTTG TCCTTCGAGG GGAGGTTGCC TACATTGATG GTCAGGTACT AGTGCCCCCT 
GGCTACGGGC AAGATGTGAG GAAGTGGCCT CAAGGCCCAG TGCCTCAGCA CCTGCCTTCA 
GCCCCACCCA GTGAAAGTAT CAAGACTCCA GAAAGACCCC GACGTGGCCT CCCAGGCCAT 
CCTGATGGGC GATTCCATCT CCCACCTCGA ATCCACCGAG CTTCTGACCC TGGCCTTCCA 
GCTGTGTTCA TCGGCCCAAG AGCCAGGACC CCACAAGGCA ACAGAGTGTG GGCTGAAGAA 
CCTAAGGAGA AACCCTCCCG CAAGACAGTG GAGCCAGATC TCGCAGGAAC TCCTGATAGC 
TGCTGCTACC CTCCTCCAGT GCCTAGGCAG GCCTCACCCC AGAACTTGGG TACTCCTAGC 
CTGTTGCATA CTCAGACTTC ACCCTTGCTG CATTCTCTAG TTGGCCAGCA TATACTGTCT 
GTTCGTCAGT TTACCAAAGA TCAGATGTCC CACCTGTTCA ATGTGGCACA CACCCTGCGG 
ATGATGGTCC AGAAGGAACG GAGCATAGAC ATTCTCAAGG GCAAGGTGAT GGCCTCCATG 
TTTTATGAAG TGAGCACTCG TACCAGCAGT TCCTTTGCAG CAGCTATGGC CCGGCTCGGA 
GGCTCTGTGC TCAACTTCTC TGAAGCTACA TCGTCTGTCC AGAAGGGAGA ATCCCTAGCA 
GATTCTGTCC AGACCATGAG CTGCTATGCC GATGTTGTTG TGCTCCGGCA CCCCCAACCT 
GGAGCTGTGG AGCTGGCAGC CAAGCACTGC CGGAGGCCAG TGATCAATGC TGGGGATGGA 
GTAGGAGAGC ACCCAACCCA GGCCTTGCTG GATGTCTTTA CCATTCGAGA GGAGCTAGGA 
ACTGTCAATG GCATGACGAT CACCATGGTA GGAGATCTCA AGCACGGACG GACAGTACAC 
TCCCTTGCCT GCCTGCTCAC ACAGTATCGG GTCAGTCTTC GCTATGTGGC ACCCCCCAGC 
CTGCGCATGC CACCAGATGT CTTGTCTTTT GTAGCTTCCA GAGGGACTAA GCAGGAGGAA 
TTTGAGAGCA TTGAGGAGGC CCTACCTGAC ACAGATGTCC TTTATATGAC TCGGATTCAA 
AAGGAACGGT TTGGATCCAC CCAAGAGTAT GAAGCTTGCT TTGGCCAATT CATCCTCACA 
CCCCACATCA TGACCCGGGC CAAGAAGAAG ATGGTCGTGA TGCACCCAAT GCCTCGGGTC 
AACGAGATCA GTGTGGAGGT AGACTCTGAC CCCCGAGCTG CCTACTTCCG GCAGGCTGAG 
AACGGCATGT ACATGCGGAT GGCCCTCCTC GCTACTGTAC TGGGTCGCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_001371087.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001371124.1 Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

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CloneID	OMc06127
Clone ID Related Accession (Same CDS sequence)	XM_001371087.3
Accession Version	XM_001371087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001371087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TGGTGTTAGA GGATGGGTCG GTCCTGCGGG GCCAGTCTTT CGGCGCGGCC 
GTATGCGCCG CCGGGGAAGT AGTGTTCCAG ACTGGCATGG TGGGCTACCC CGAGGCCCTC 
ACCGATCCCT CCTACAAAGC CCAGATCTTG GTCCTGACGT ATCCGCTCGT GGGCAACTAT 
GGCATCCCCC CAGATGAGGA AGACGAGTTC AACCTCAGCC AGTGGTTTGA GTCATCAGGG 
ATTCATGTGG CAGGACTTGT GGTGGGAGAA TGCTGCTCTA CACCTAGCCA CTGGAGCGCC 
ACCCGAACCT TGCACCAGTG GCTGGAGGAG CATGGTATAC CTGGTCTCCA AGGAGTTGAT 
ACTAGGGAAC TGACAAAGAA GTTGCGGGAA CAAGGATCAC TTCTGGGAAA GCTGGTGCAG 
GATGGGATAG AGCCTTCATC CCTACCATTT TTGGATCCCA ATGCTCGAGC TCTTGTACCT 
GAGGTTTCCA TTAAGGAACC AAGGATATAC AATGCAAGGG GGTCTCCACG AATTCTTGCT 
TTGGATTGTG GGCTCAAATA CAGTCAGATT AGATGCTTGT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTGC CCTGGGACTA CAAATTAGAC AGCCATGAGT ATGAAGGTCT GTTCCTAAGT 
AATGGCCCTG GAGACCCTGC TTCTTGCCCT AATGTGGTAT CTATGCTGAG CAATGTCCTT 
TCTGAGCCAG ACCCCCGACC TGTCTTTGGT ATCTGCCTGG GTCACCAGCT ATTGGCCTTA 
GCCATCGGAG CCAAGACTTA CAAAATGAGG TATGGCAACC GTGGCCACAA CCAGCCATGC 
CTGCTAAAGG GCTCTGGACG GTGTTTCTTA ACATCTCAGA ATCATGGCTT TGCAGTGGAA 
ACAGAATCTC TGCCACCAGG CTGGACCCCT CTTTTCACAA ATGCCAATGA TCTTTCCAAT 
GAAGGCATTG TACATGAGAC CCTGCCCTTC TTCAGCGTCC AGTTCCACCC TGAGCACCGA 
GCCGGCCCCT CTGATATGGA GTTGCTTTTT GACATCTTCC TGGAGACCGT GAAGGAGAAC 
AGGGCTGGGG GCAAGACAGT TCGTGAAAGG TTAATGGAGC GTCTCTGTCC CTCGGGTACC 
CCTACCCCAA GAGACATGCC TCCATCTCCC CGAAAAGTGC TGATCCTGGG CTCAGGGGGT 
CTCTCCATTG GCCAGGCTGG AGAGTTTGAC TACTCTGGCT CTCAGGCAAT CAAGGCCCTA 
AAGGAGGAAA ATATTCAAAC GCTGCTGATA AATCCTAATA TTGCCACAGT GCAAACCTCC 
CAGGGACTGG CGGACAAAGT CTATTTCTTG CCTATAACTC CTCATTATGT GACTCAGGTT 
ATTCGTAATG AGCGTCCAGA TGGTGTATTA CTGACATTTG GGGGCCAGAC AGCACTGAAT 
TGTGGTGTGG AGCTGACTAA GGCTGGGGTT CTGGCCCGGT ATGGAGTACG GGTCCTAGGC 
ACCCCCGTAG AGACAATTGA GCTGACTGAG GATCGGCGTG CTTTTGCAGC AAGGATGGCA 
GAAATTGGAG AACATGTGGC CCCGAGCGAG GCTGCAAACT CCCTGGAACA GGCGCAAGCT 
GCTGCAGAAC GACTGGGGTA CCCGGTCCTG GTACGTGCTG CCTTTGCCCT AGGTGGCCTT 
GGCTCCGGCT TTGCTTCTAG CAGAGAAGAA CTCTCCTCTC TGGTGGCCCC AGCCTTTGCT 
CACACTAGTC AAGTACTGGT GGACAAGTCC CTCAAGGGAT GGAAAGAAAT AGAATATGAA 
GTGGTGAGAG ATGCCTATGG CAACTGTGTC ACGGTATGTA ACATGGAAAA TCTAGATCCA 
CTGGGCATTC ACACTGGTGA ATCTATAGTT GTGGCCCCAA GCCAGACACT AAACGACAGG 
GAATATCAGC TGTTGCGACA GACTGCTATC AAGGTGACTC AACACCTGGG CATTGTTGGG 
GAGTGCAATG TACAATATGC CTTGAACCCT GAGTCTGAAC AGTACTATAT CATTGAAGTG 
AATGCACGAC TGTCTCGGAG TTCGGCCCTT GCCAGTAAAG CCACTGGTTA TCCTCTGGCC 
TATGTAGCAG CCAAGCTGGC TTTGGGTATC TCCTTGCCTG ATCTCAGGAA CTCGGTAACT 
GGGGGTACTG CTGCTTTTGA GCCCAGCCTG GATTACTGCG TGGTTAAGGT CCCTCGCTGG 
GACCTTAGCA AGTTCCTCCG AGTTAGCACA AGGATTGGAA GCTGCATGAA GAGTGTTGGT 
GAGGTCATGG CTATTGGACG TGGGTTCGAA GAGGCATTCC AAAAGGCCTT GCGCATGGTG 
GATGAGAACT GTGTTGGCTT TGATCACACA ATGAAGCCCG TCAGTGACAT GGAGCTGGAA 
ACGCCAACAG ACAAGCGGAT CTTTGTGGTA GCAGCTGCTT TGTGGGCAGG ATATACCATA 
GACCGACTGT ATGAGCTTAC TCGCATTGAT CGCTGGTTCC TGCAGTGCAT GAAACGCATT 
ATAGAGCACA CTCGAAAACT AGAGGAACAC CGTGGGCAGC CCTTGCCCCC TGATTTGCTG 
CACCAGGCCA AGCGCCTTGG ATTCTCCGAC AAGCAGATTG CCCTCGCAGT ACTCAGCACA 
GAGCTGGCCG TACGAAAACT ACGGCAGGAG CTGGGCATCT GTCCAGCGGT CAAGCAGATT 
GATACAGTGG CAGCAGAGTG GCCAGCTCAA ACCAACTATC TGTACCTGAC TTATGGTGGC 
ACCACCCATG ACCTGGCTTT CCACGCTCCC CATGTCCTGG TCTTGGGCTC TGGCGTTTAC 
CGCATTGGCT CCAGTGTGGA ATTTGACTGG TGTGCTGTGG GCTGCATCCG GCAGCTGCGC 
AAGATGGGGT ATAAGACTAT TATGGTGAAC TATAACCCTG AGACAGTCAG TACTGATTAT 
GACATGTGTG ATCGGCTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGATATCTAT 
GAGCTAGAAA ACCCTGAGGG TGTAATCCTC TCCATGGGAG GTCAGTTGCC CAACAACATG 
GCAATGGCCC TGCATAGGCA GCAGTGCCGA GTCCTTGGCA CATCCCCAGA AGCCATCGAC 
TCTGCTGAGA ACAGATTCAA ATTTTCCCGA CTTCTTGACA CCATTGGCAT CAGTCAGCCT 
CAGTGGCGAG AACTCAGTGA CTTGGAGTCT GCTCGCCAGT TCTGTCACAC CGTGGGGTAC 
CCTTGTGTTG TCCGCCCATC CTATGTGCTG AGTGGTGCAG CTATGAATGT GGCCTATACT 
GATGGAGACC TTGAGCGATT TCTGAGCAGT GCAGCTGCTG TCTCCAAAGA ACATCCTGTG 
GTCATCTCCA AGTTCATCCA AGAGGCCAAG GAAATTGACG TGGATGCTGT GGCTTGTGAT 
GGCATGGTGG TTGCTGTAGC TATCTCAGAG CATGTGGAGA ATGCAGGGGT GCACTCAGGG 
GATGCCACTT TGGTGACTCC TCCTCAAGAC ATCACACCTA AGACATTGGA AAGGATCAAA 
GCAATTGTTC ACGCTGTGGG TCAAGAGCTA CAGGTCACAG GGCCTTTCAA TCTTCAGCTC 
ATTGCCAAGG ATGACCAGCT CAAGGTTATC GAGTGCAATG TCCGTGTCTC TCGGTCCTTC 
CCCTTTGTCT CTAAGACACT TGGAGTAGAT CTGGTAGCCC TGGCTACCCG AATTATCATG 
GGTGAGGAGG TGGAGCCTGT TGGGCTCATG GCTGGAACTG GAGTAGTGGG AGTTAAGGTG 
CCCCAGTTCT CATTCTCACG CCTGGCAGGA GCAGATGTGG TGCTGGGTGT AGAGATGACC 
AGCACTGGGG AAGTGGCTGG CTTTGGGGAG AGCCGCTGTG AGGCCTACCT CAAGGCTATG 
CTAAGCACTG GCTTCAAGAT CCCCAAGAAG AATATCCTGC TGACTATTGG TAGTTACAAG 
AACAAGAGTG AGCTGCTCCC TACAGTGAGG CTGTTGGAGA GTCTAGGCTA TAGCTTATAT 
GCCAGTTTGG GCACAGCAGA CTTCTACACA GAGCATGGTG TCAAGGTTAC AGCCGTGGAC 
TGGCACTTTG AGGAGGCAGT TGAAGGTGAA TGTCCTCCAC AACGAAGCAT CCTGGAACAG 
TTGGCAGAAA ACCACTTTGA ACTGGTCATC AACCTGTCAA TGCGAGGGGC AGGGGGTCGA 
CGCCTCTCCT CCTTTGTAAC CAAGGGGTAC CGGACCCGTC GCCTGGCTGC AGACTTCTCT 
GTGCCACTCA TCATTGACAT CAAGTGCACT AAGCTATTTG TAGAGGCATT GGGCCAGATT 
GGGCCAGCCC CTCCCCTGAA GGTGCATGTT GACTGTATGA CCTCACAGAA GCTTGTGCGT 
CTGCCAGGGC TGATTGATGT ACATGTGCAC CTACGGGAAC CAGGTGGTAC TCACAAGGAG 
GACTTTGCCT CAGGCACAGC GGCTGCCCTA GCTGGTGGGG TCACCATGGT GTGTGCTATG 
CCCAACACTC GACCTCCCAT CATAGATGCC CCAGCCCTTG CCCTTGCACA GAAGTTGGCA 
GAAACGGGGG CCCGCTGTGA CTATGCCTTG TTCCTTGGGG CCTCGTCAGA GAATGCAGGA 
ACCCTGGGTG CAGTGGCTGG GGCAGCTGCA GGATTGAAGC TCTATCTCAA TGAGACATTC 
TCTGAACTTC AACTGGAGAG CATCTCTCAT TGGATGGAGC ACTTTGAGAC ATGGCCACAC 
CATCTCCCCA TTGTGGCTCA TGCAGAACGA CAGACCGTAG CTGCCATCCT TATGGTAGCC 
CAGTTGGCTC AGCGACCTGT CCACATCTGC CACGTGGCCC GGAAGGAGGA GATCCTATTA 
ATTAAAGCTG CAAAGGCACG GGGGGTATCA GTGACCTGCG AGGTGGCCCC CCACCATCTC 
TTCCTCAGCC AAGCAGACCT TGAGCGCCTG GGGCCTGGGA AAGGTGAAGT CCGGCCTGAG 
CTCGGCACCC TTGAGGACGT GGAGGCTCTC TGGGAGAACA TGGCCATCAT TGATTGCTTT 
GCCTCAGACC ATGCTCCTCA CACCCTGGAA GAAAAATCTG GGTCCAAGCC TCCCCCTGGC 
TTCCCAGGTC TGGAGACAAT GCTTCCATTG CTGCTAACAG CGGTGAGCGA GGGCCGACTC 
AGCCTAGATG ACCTGCTGCA GAAGCTGCAC CACAATCCCC GACGGATCTT TCACTTGCCC 
CAGCAGGAGG ACACCTATGT TGAGGTGGAT CTGGAGCATG AATGGATCAT CCCCAGCCAC 
ATGCCCTTCT CCAAGGCACA TTGGACACCT TTTGAGGGAC AGCGAGTGAA GGGCACAATA 
CGGCGAGTTG TCCTTCGAGG GGAGGTTGCC TACATTGATG GTCAGGTACT AGTGCCCCCT 
GGCTACGGGC AAGATGTGAG GAAGTGGCCT CAAGGCCCAG TGCCTCAGCA CCTGCCTTCA 
GCCCCACCCA GTGAAAGTAT CAAGACTCCA GAAAGACCCC GACGTGGCCT CCCAGGCCAT 
CCTGATGGGC GATTCCATCT CCCACCTCGA ATCCACCGAG CTTCTGACCC TGGCCTTCCA 
GCTGAAGAAC CTAAGGAGAA ACCCTCCCGC AAGACAGTGG AGCCAGATCT CGCAGGAACT 
CCTGATAGCT GCTGCTACCC TCCTCCAGTG CCTAGGCAGG CCTCACCCCA GAACTTGGGT 
ACTCCTAGCC TGTTGCATAC TCAGACTTCA CCCTTGCTGC ATTCTCTAGT TGGCCAGCAT 
ATACTGTCTG TTCGTCAGTT TACCAAAGAT CAGATGTCCC ACCTGTTCAA TGTGGCACAC 
ACCCTGCGGA TGATGGTCCA GAAGGAACGG AGCATAGACA TTCTCAAGGG CAAGGTGATG 
GCCTCCATGT TTTATGAAGT GAGCACTCGT ACCAGCAGTT CCTTTGCAGC AGCTATGGCC 
CGGCTCGGAG GCTCTGTGCT CAACTTCTCT GAAGCTACAT CGTCTGTCCA GAAGGGAGAA 
TCCCTAGCAG ATTCTGTCCA GACCATGAGC TGCTATGCCG ATGTTGTTGT GCTCCGGCAC 
CCCCAACCTG GAGCTGTGGA GCTGGCAGCC AAGCACTGCC GGAGGCCAGT GATCAATGCT 
GGGGATGGAG TAGGAGAGCA CCCAACCCAG GCCTTGCTGG ATGTCTTTAC CATTCGAGAG 
GAGCTAGGAA CTGTCAATGG CATGACGATC ACCATGGTAG GAGATCTCAA GCACGGACGG 
ACAGTACACT CCCTTGCCTG CCTGCTCACA CAGTATCGGG TCAGTCTTCG CTATGTGGCA 
CCCCCCAGCC TGCGCATGCC ACCAGATGTC TTGTCTTTTG TAGCTTCCAG AGGGACTAAG 
CAGGAGGAAT TTGAGAGCAT TGAGGAGGCC CTACCTGACA CAGATGTCCT TTATATGACT 
CGGATTCAAA AGGAACGGTT TGGATCCACC CAAGAGTATG AAGCTTGCTT TGGCCAATTC 
ATCCTCACAC CCCACATCAT GACCCGGGCC AAGAAGAAGA TGGTCGTGAT GCACCCAATG 
CCTCGGGTCA ACGAGATCAG TGTGGAGGTA GACTCTGACC CCCGAGCTGC CTACTTCCGG 
CAGGCTGAGA ACGGCATGTA CATGCGGATG GCCCTCCTCG CTACTGTACT GGGTCGCTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001371124.1
CDS	197..6859
Translation	MAALVLEDGSVLRGQSFGAAVCAAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLVGNYGIPPDEEDEFNLSQWFESSGIHVAGLVVGECCSTPSHWSATRTLHQWLEEHGIPGLQGVDTRELTKKLREQGSLLGKLVQDGIEPSSLPFLDPNARALVPEVSIKEPRIYNARGSPRILALDCGLKYSQIRCLCQRGAEVTVVPWDYKLDSHEYEGLFLSNGPGDPASCPNVVSMLSNVLSEPDPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLKGSGRCFLTSQNHGFAVETESLPPGWTPLFTNANDLSNEGIVHETLPFFSVQFHPEHRAGPSDMELLFDIFLETVKENRAGGKTVRERLMERLCPSGTPTPRDMPPSPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASSREELSSLVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGISLPDLRNSVTGGTAAFEPSLDYCVVKVPRWDLSKFLRVSTRIGSCMKSVGEVMAIGRGFEEAFQKALRMVDENCVGFDHTMKPVSDMELETPTDKRIFVVAAALWAGYTIDRLYELTRIDRWFLQCMKRIIEHTRKLEEHRGQPLPPDLLHQAKRLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYGGTTHDLAFHAPHVLVLGSGVYRIGSSVEFDWCAVGCIRQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGMVVAVAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEEVEPVGLMAGTGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVEGECPPQRSILEQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAETGARCDYALFLGASSENAGTLGAVAGAAAGLKLYLNETFSELQLESISHWMEHFETWPHHLPIVAHAERQTVAAILMVAQLAQRPVHICHVARKEEILLIKAAKARGVSVTCEVAPHHLFLSQADLERLGPGKGEVRPELGTLEDVEALWENMAIIDCFASDHAPHTLEEKSGSKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQKLHHNPRRIFHLPQQEDTYVEVDLEHEWIIPSHMPFSKAHWTPFEGQRVKGTIRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGPVPQHLPSAPPSESIKTPERPRRGLPGHPDGRFHLPPRIHRASDPGLPAEEPKEKPSRKTVEPDLAGTPDSCCYPPPVPRQASPQNLGTPSLLHTQTSPLLHSLVGQHILSVRQFTKDQMSHLFNVAHTLRMMVQKERSIDILKGKVMASMFYEVSTRTSSSFAAAMARLGGSVLNFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDVFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPDVLSFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYMRMALLATVLGRY

Target ORF information:

RefSeq Version	XM_001371087.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001371087.3

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTAGA GGATGGGTCG GTCCTGCGGG GCCAGTCTTT CGGCGCGGCC 
GTATGCGCCG CCGGGGAAGT AGTGTTCCAG ACTGGCATGG TGGGCTACCC CGAGGCCCTC 
ACCGATCCCT CCTACAAAGC CCAGATCTTG GTCCTGACGT ATCCGCTCGT GGGCAACTAT 
GGCATCCCCC CAGATGAGGA AGACGAGTTC AACCTCAGCC AGTGGTTTGA GTCATCAGGG 
ATTCATGTGG CAGGACTTGT GGTGGGAGAA TGCTGCTCTA CACCTAGCCA CTGGAGCGCC 
ACCCGAACCT TGCACCAGTG GCTGGAGGAG CATGGTATAC CTGGTCTCCA AGGAGTTGAT 
ACTAGGGAAC TGACAAAGAA GTTGCGGGAA CAAGGATCAC TTCTGGGAAA GCTGGTGCAG 
GATGGGATAG AGCCTTCATC CCTACCATTT TTGGATCCCA ATGCTCGAGC TCTTGTACCT 
GAGGTTTCCA TTAAGGAACC AAGGATATAC AATGCAAGGG GGTCTCCACG AATTCTTGCT 
TTGGATTGTG GGCTCAAATA CAGTCAGATT AGATGCTTGT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTGC CCTGGGACTA CAAATTAGAC AGCCATGAGT ATGAAGGTCT GTTCCTAAGT 
AATGGCCCTG GAGACCCTGC TTCTTGCCCT AATGTGGTAT CTATGCTGAG CAATGTCCTT 
TCTGAGCCAG ACCCCCGACC TGTCTTTGGT ATCTGCCTGG GTCACCAGCT ATTGGCCTTA 
GCCATCGGAG CCAAGACTTA CAAAATGAGG TATGGCAACC GTGGCCACAA CCAGCCATGC 
CTGCTAAAGG GCTCTGGACG GTGTTTCTTA ACATCTCAGA ATCATGGCTT TGCAGTGGAA 
ACAGAATCTC TGCCACCAGG CTGGACCCCT CTTTTCACAA ATGCCAATGA TCTTTCCAAT 
GAAGGCATTG TACATGAGAC CCTGCCCTTC TTCAGCGTCC AGTTCCACCC TGAGCACCGA 
GCCGGCCCCT CTGATATGGA GTTGCTTTTT GACATCTTCC TGGAGACCGT GAAGGAGAAC 
AGGGCTGGGG GCAAGACAGT TCGTGAAAGG TTAATGGAGC GTCTCTGTCC CTCGGGTACC 
CCTACCCCAA GAGACATGCC TCCATCTCCC CGAAAAGTGC TGATCCTGGG CTCAGGGGGT 
CTCTCCATTG GCCAGGCTGG AGAGTTTGAC TACTCTGGCT CTCAGGCAAT CAAGGCCCTA 
AAGGAGGAAA ATATTCAAAC GCTGCTGATA AATCCTAATA TTGCCACAGT GCAAACCTCC 
CAGGGACTGG CGGACAAAGT CTATTTCTTG CCTATAACTC CTCATTATGT GACTCAGGTT 
ATTCGTAATG AGCGTCCAGA TGGTGTATTA CTGACATTTG GGGGCCAGAC AGCACTGAAT 
TGTGGTGTGG AGCTGACTAA GGCTGGGGTT CTGGCCCGGT ATGGAGTACG GGTCCTAGGC 
ACCCCCGTAG AGACAATTGA GCTGACTGAG GATCGGCGTG CTTTTGCAGC AAGGATGGCA 
GAAATTGGAG AACATGTGGC CCCGAGCGAG GCTGCAAACT CCCTGGAACA GGCGCAAGCT 
GCTGCAGAAC GACTGGGGTA CCCGGTCCTG GTACGTGCTG CCTTTGCCCT AGGTGGCCTT 
GGCTCCGGCT TTGCTTCTAG CAGAGAAGAA CTCTCCTCTC TGGTGGCCCC AGCCTTTGCT 
CACACTAGTC AAGTACTGGT GGACAAGTCC CTCAAGGGAT GGAAAGAAAT AGAATATGAA 
GTGGTGAGAG ATGCCTATGG CAACTGTGTC ACGGTATGTA ACATGGAAAA TCTAGATCCA 
CTGGGCATTC ACACTGGTGA ATCTATAGTT GTGGCCCCAA GCCAGACACT AAACGACAGG 
GAATATCAGC TGTTGCGACA GACTGCTATC AAGGTGACTC AACACCTGGG CATTGTTGGG 
GAGTGCAATG TACAATATGC CTTGAACCCT GAGTCTGAAC AGTACTATAT CATTGAAGTG 
AATGCACGAC TGTCTCGGAG TTCGGCCCTT GCCAGTAAAG CCACTGGTTA TCCTCTGGCC 
TATGTAGCAG CCAAGCTGGC TTTGGGTATC TCCTTGCCTG ATCTCAGGAA CTCGGTAACT 
GGGGGTACTG CTGCTTTTGA GCCCAGCCTG GATTACTGCG TGGTTAAGGT CCCTCGCTGG 
GACCTTAGCA AGTTCCTCCG AGTTAGCACA AGGATTGGAA GCTGCATGAA GAGTGTTGGT 
GAGGTCATGG CTATTGGACG TGGGTTCGAA GAGGCATTCC AAAAGGCCTT GCGCATGGTG 
GATGAGAACT GTGTTGGCTT TGATCACACA ATGAAGCCCG TCAGTGACAT GGAGCTGGAA 
ACGCCAACAG ACAAGCGGAT CTTTGTGGTA GCAGCTGCTT TGTGGGCAGG ATATACCATA 
GACCGACTGT ATGAGCTTAC TCGCATTGAT CGCTGGTTCC TGCAGTGCAT GAAACGCATT 
ATAGAGCACA CTCGAAAACT AGAGGAACAC CGTGGGCAGC CCTTGCCCCC TGATTTGCTG 
CACCAGGCCA AGCGCCTTGG ATTCTCCGAC AAGCAGATTG CCCTCGCAGT ACTCAGCACA 
GAGCTGGCCG TACGAAAACT ACGGCAGGAG CTGGGCATCT GTCCAGCGGT CAAGCAGATT 
GATACAGTGG CAGCAGAGTG GCCAGCTCAA ACCAACTATC TGTACCTGAC TTATGGTGGC 
ACCACCCATG ACCTGGCTTT CCACGCTCCC CATGTCCTGG TCTTGGGCTC TGGCGTTTAC 
CGCATTGGCT CCAGTGTGGA ATTTGACTGG TGTGCTGTGG GCTGCATCCG GCAGCTGCGC 
AAGATGGGGT ATAAGACTAT TATGGTGAAC TATAACCCTG AGACAGTCAG TACTGATTAT 
GACATGTGTG ATCGGCTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGATATCTAT 
GAGCTAGAAA ACCCTGAGGG TGTAATCCTC TCCATGGGAG GTCAGTTGCC CAACAACATG 
GCAATGGCCC TGCATAGGCA GCAGTGCCGA GTCCTTGGCA CATCCCCAGA AGCCATCGAC 
TCTGCTGAGA ACAGATTCAA ATTTTCCCGA CTTCTTGACA CCATTGGCAT CAGTCAGCCT 
CAGTGGCGAG AACTCAGTGA CTTGGAGTCT GCTCGCCAGT TCTGTCACAC CGTGGGGTAC 
CCTTGTGTTG TCCGCCCATC CTATGTGCTG AGTGGTGCAG CTATGAATGT GGCCTATACT 
GATGGAGACC TTGAGCGATT TCTGAGCAGT GCAGCTGCTG TCTCCAAAGA ACATCCTGTG 
GTCATCTCCA AGTTCATCCA AGAGGCCAAG GAAATTGACG TGGATGCTGT GGCTTGTGAT 
GGCATGGTGG TTGCTGTAGC TATCTCAGAG CATGTGGAGA ATGCAGGGGT GCACTCAGGG 
GATGCCACTT TGGTGACTCC TCCTCAAGAC ATCACACCTA AGACATTGGA AAGGATCAAA 
GCAATTGTTC ACGCTGTGGG TCAAGAGCTA CAGGTCACAG GGCCTTTCAA TCTTCAGCTC 
ATTGCCAAGG ATGACCAGCT CAAGGTTATC GAGTGCAATG TCCGTGTCTC TCGGTCCTTC 
CCCTTTGTCT CTAAGACACT TGGAGTAGAT CTGGTAGCCC TGGCTACCCG AATTATCATG 
GGTGAGGAGG TGGAGCCTGT TGGGCTCATG GCTGGAACTG GAGTAGTGGG AGTTAAGGTG 
CCCCAGTTCT CATTCTCACG CCTGGCAGGA GCAGATGTGG TGCTGGGTGT AGAGATGACC 
AGCACTGGGG AAGTGGCTGG CTTTGGGGAG AGCCGCTGTG AGGCCTACCT CAAGGCTATG 
CTAAGCACTG GCTTCAAGAT CCCCAAGAAG AATATCCTGC TGACTATTGG TAGTTACAAG 
AACAAGAGTG AGCTGCTCCC TACAGTGAGG CTGTTGGAGA GTCTAGGCTA TAGCTTATAT 
GCCAGTTTGG GCACAGCAGA CTTCTACACA GAGCATGGTG TCAAGGTTAC AGCCGTGGAC 
TGGCACTTTG AGGAGGCAGT TGAAGGTGAA TGTCCTCCAC AACGAAGCAT CCTGGAACAG 
TTGGCAGAAA ACCACTTTGA ACTGGTCATC AACCTGTCAA TGCGAGGGGC AGGGGGTCGA 
CGCCTCTCCT CCTTTGTAAC CAAGGGGTAC CGGACCCGTC GCCTGGCTGC AGACTTCTCT 
GTGCCACTCA TCATTGACAT CAAGTGCACT AAGCTATTTG TAGAGGCATT GGGCCAGATT 
GGGCCAGCCC CTCCCCTGAA GGTGCATGTT GACTGTATGA CCTCACAGAA GCTTGTGCGT 
CTGCCAGGGC TGATTGATGT ACATGTGCAC CTACGGGAAC CAGGTGGTAC TCACAAGGAG 
GACTTTGCCT CAGGCACAGC GGCTGCCCTA GCTGGTGGGG TCACCATGGT GTGTGCTATG 
CCCAACACTC GACCTCCCAT CATAGATGCC CCAGCCCTTG CCCTTGCACA GAAGTTGGCA 
GAAACGGGGG CCCGCTGTGA CTATGCCTTG TTCCTTGGGG CCTCGTCAGA GAATGCAGGA 
ACCCTGGGTG CAGTGGCTGG GGCAGCTGCA GGATTGAAGC TCTATCTCAA TGAGACATTC 
TCTGAACTTC AACTGGAGAG CATCTCTCAT TGGATGGAGC ACTTTGAGAC ATGGCCACAC 
CATCTCCCCA TTGTGGCTCA TGCAGAACGA CAGACCGTAG CTGCCATCCT TATGGTAGCC 
CAGTTGGCTC AGCGACCTGT CCACATCTGC CACGTGGCCC GGAAGGAGGA GATCCTATTA 
ATTAAAGCTG CAAAGGCACG GGGGGTATCA GTGACCTGCG AGGTGGCCCC CCACCATCTC 
TTCCTCAGCC AAGCAGACCT TGAGCGCCTG GGGCCTGGGA AAGGTGAAGT CCGGCCTGAG 
CTCGGCACCC TTGAGGACGT GGAGGCTCTC TGGGAGAACA TGGCCATCAT TGATTGCTTT 
GCCTCAGACC ATGCTCCTCA CACCCTGGAA GAAAAATCTG GGTCCAAGCC TCCCCCTGGC 
TTCCCAGGTC TGGAGACAAT GCTTCCATTG CTGCTAACAG CGGTGAGCGA GGGCCGACTC 
AGCCTAGATG ACCTGCTGCA GAAGCTGCAC CACAATCCCC GACGGATCTT TCACTTGCCC 
CAGCAGGAGG ACACCTATGT TGAGGTGGAT CTGGAGCATG AATGGATCAT CCCCAGCCAC 
ATGCCCTTCT CCAAGGCACA TTGGACACCT TTTGAGGGAC AGCGAGTGAA GGGCACAATA 
CGGCGAGTTG TCCTTCGAGG GGAGGTTGCC TACATTGATG GTCAGGTACT AGTGCCCCCT 
GGCTACGGGC AAGATGTGAG GAAGTGGCCT CAAGGCCCAG TGCCTCAGCA CCTGCCTTCA 
GCCCCACCCA GTGAAAGTAT CAAGACTCCA GAAAGACCCC GACGTGGCCT CCCAGGCCAT 
CCTGATGGGC GATTCCATCT CCCACCTCGA ATCCACCGAG CTTCTGACCC TGGCCTTCCA 
GCTGAAGAAC CTAAGGAGAA ACCCTCCCGC AAGACAGTGG AGCCAGATCT CGCAGGAACT 
CCTGATAGCT GCTGCTACCC TCCTCCAGTG CCTAGGCAGG CCTCACCCCA GAACTTGGGT 
ACTCCTAGCC TGTTGCATAC TCAGACTTCA CCCTTGCTGC ATTCTCTAGT TGGCCAGCAT 
ATACTGTCTG TTCGTCAGTT TACCAAAGAT CAGATGTCCC ACCTGTTCAA TGTGGCACAC 
ACCCTGCGGA TGATGGTCCA GAAGGAACGG AGCATAGACA TTCTCAAGGG CAAGGTGATG 
GCCTCCATGT TTTATGAAGT GAGCACTCGT ACCAGCAGTT CCTTTGCAGC AGCTATGGCC 
CGGCTCGGAG GCTCTGTGCT CAACTTCTCT GAAGCTACAT CGTCTGTCCA GAAGGGAGAA 
TCCCTAGCAG ATTCTGTCCA GACCATGAGC TGCTATGCCG ATGTTGTTGT GCTCCGGCAC 
CCCCAACCTG GAGCTGTGGA GCTGGCAGCC AAGCACTGCC GGAGGCCAGT GATCAATGCT 
GGGGATGGAG TAGGAGAGCA CCCAACCCAG GCCTTGCTGG ATGTCTTTAC CATTCGAGAG 
GAGCTAGGAA CTGTCAATGG CATGACGATC ACCATGGTAG GAGATCTCAA GCACGGACGG 
ACAGTACACT CCCTTGCCTG CCTGCTCACA CAGTATCGGG TCAGTCTTCG CTATGTGGCA 
CCCCCCAGCC TGCGCATGCC ACCAGATGTC TTGTCTTTTG TAGCTTCCAG AGGGACTAAG 
CAGGAGGAAT TTGAGAGCAT TGAGGAGGCC CTACCTGACA CAGATGTCCT TTATATGACT 
CGGATTCAAA AGGAACGGTT TGGATCCACC CAAGAGTATG AAGCTTGCTT TGGCCAATTC 
ATCCTCACAC CCCACATCAT GACCCGGGCC AAGAAGAAGA TGGTCGTGAT GCACCCAATG 
CCTCGGGTCA ACGAGATCAG TGTGGAGGTA GACTCTGACC CCCGAGCTGC CTACTTCCGG 
CAGGCTGAGA ACGGCATGTA CATGCGGATG GCCCTCCTCG CTACTGTACT GGGTCGCTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CAD cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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