NF1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NF1
Entrez Gene ID	100023872
Full Name	neurofibromin 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016429243.1	XP_016284729.1	neurofibromin

Pathways from BioSystems
Gene Ontology

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMc39514	XM_016429243.1 Latest version!	Monodelphis domestica neurofibromin 1 (NF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NF1 ( XM_016429243.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016284729.1 Monodelphis domestica neurofibromin 1 (NF1), mRNA.

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CloneID	OMc39514
Clone ID Related Accession (Same CDS sequence)	XM_016429243.1
Accession Version	XM_016429243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	neurofibromin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT GTGCAGAAAA ATTATTTGAC TTGGTGGATG GTTTTGCTGA AAGCACCAAA 
CGTAAAGCTG CAGTTTGGCC ACTGCAAATC ATTCTTCTTG TCTTGTGTCC AGAAATAATT 
CAGGACATTT CCAAGGATGT GGTTGATGAA GCAAAAATGA ATAAGAAAAT GTTTCTTGAC 
AATCTCAGAA GAGCGCTAGC AGGCCATGGT GGGAGCAGGC AGCTGACGGA GAGCGCAGCC 
ATCGCTTGTG TGAAGCTATG CAAAGCCAGC ACCTACATCA ACTGGGAGGA TAACTCAGTC 
ATCTTCCTGC TTGTCCAGTC CATGGTGGTA GACCTGAAGA ACTTGCTTTT TAATCCAAGC 
AAACCGTTCT CGAGAGGCAG TCAAAATGCT GACGTGGACC TCATGATTGA CTGCTTGGTT 
TCTTGCTTTC GTATAAGCCC TCACAATAAC CAACACTTTA AGATCTGTTT GGCCCAGAAC 
TCCCCATCCA CATTTCATTA TGTGCTGGTA AACTCCCTCC ATCGAATTAT CACCAATAGC 
ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCACTGGATT GGTGGCCCAA GATTGATGCT 
GTGTATTGCC ATTCTGTTGA ACTACGAAAC ATGTTTGGTG AAACACTTCA TAAGGCCGTG 
CAGGGCTGTG GGGCACATGC CGCGATACGA ATGGCACCGA TGCCAACCAG TCACTGTTTT 
GCGTGGCAGT GGGCTTCTGT GGCTAGTCTT ACATTTAAAG AAAAAATGAC AAGCCTTAAA 
TTTAAAGAAA AACCTACGGA CTTGGAAACA AGAAGCTATA AATACCTCCT ATTATCCATG 
GTGAAACTAA TCCATGCAGA CCCTAAGCTG TTGCTATGTA ATCCAAGAAA ACAAGGGCCA 
GAATCTCAGG GGAGTACAGC AGAGTTAATC ACAGGCCTTG TGCAGCTTGT CCCACAGTCT 
AGCATGCCAG AAATAGCCCA AGAAGCAATG GAGGCTTTGC TGGTTCTTCA TCAGTTGGAT 
AGCATTGACT TATGGAATCC AGATTCACCT ATAGAAACAT TCTGGGAGAT TAGCTCACAA 
ATGCTTTTTT ATATCTGTAA AAAGCTAACT ACCCATCAGA TGCTTAGCAG TACAGAAATT 
CTCAAATGGT TGCGGGAAAT TTTGATCTGT AGGAACAAAT ATCTTCTTAA AAATAAGCAG 
TCAGATAGGA GTTCTTCCCA TTTTCTCTAC CTTTATGGGG TAGGGTATGA TGGCTCTTCT 
GGTGGCGGTA CCAGTCATAT CTCTCTAGAC CATGAAGAGT TTATGCGTGC CTGTGCTCCA 
GGACCACCTC TCAGGAAAGT AAAAGGAAAC TCCTTGATGG ATGGTGCAAT AGGGTCCAGT 
GGGACGCCAA TATGTCGCCA GGCTCAGACT AAGCTGGAGG TCGCTCTGTA CATGTTTCTG 
TGGAGCCCCG ACATTGAAGC TGTGCTTGTG GCCATGTCCT GCTTTCGACA CCTCTGTGAG 
GAAGCCGATA TCAGGTGTGG CATAGATGAG ATTTCCGTGC ATCATTTCTT GCCTAACTAT 
AATACCTTCA TGGAATTTGC GTCTGTCAGC AACATGATGT CAACAGGAAG AGCAGCTCTT 
CAGAAAAGAG TGATGGCATT GCTAAGGCGC ATCGAACACC CGACTGCAGG CAACACCGAG 
GCTTGGGAAG ATACTTATAC AAAATGGGAA CAAGCTACAA AATCAATCCT CAATTATCCA 
AAAGCCAAAA TGGAAGATAG CCAGGTTGCT GAAAGTCTTC ATAAGACCAT TGTGAAAAGG 
AGGATGTCTC ATGTGAGTGG AGGAGGCTCC ATAGACTTGT GTGACACAGA CTCACTCCAA 
GAGTGGATCA ACATGACTGG TTTTCTTTGT GCCCTCGGTG GGGTGTGCCT GCAGCACCGA 
AGCAATGTTG GCTTTGCCAC CTACAGCCCT CCAATGGGCC CCATCAGTGA GCGCAAGGGC 
TCTCTGATTT CCATGGTGTC CTCAGATGGA AACGCAGAGA CACCCGTCAG CAAGTTCATG 
GATCGGCTGC TCTCCTTGAT GGTGTGTAAC CATGAAAAAG TGGGGCTGCA GATACGGAGC 
AACGTCAAGG ATTTGTTGGG CCTAGAATTG AGTCCTGCCC TTTATCCAAT GCTGTTTAAC 
AAATTAAAAA ATACCATCAG CAAGTTTTTT GATCCTCAAG GCCAGGTTTT ATTGACTGAC 
ACCAATACCC AATTTGTGGA ACAGACCATA GCCATAATGA AGAACTTGCT CGACAATCAC 
AGTGAGGGCA GTTCTGAACA CATGGGGCAA GCTAGCATCG AAATAATGAT GTTAAATCTG 
GTCAGATACG TTAGAGTGTT TGGGAATTTG GTACACGCAA TTCAGATAAA AACTAAACTC 
TGCCAGCTGG TTCAAGTGAT GATGGAGAGG AGAGATGACC TTTCATTTTG CCAGGAGATG 
AAATTTAGGA ACAAAATGGT GGAATATCTG ACAGATTGGG TCATGGGGAC ATCTAACCAA 
GCAACAGATG AAGATATAAA ATGTCTAACT AGAGATTTGG ACCAGGCAAG CATGGAAGCA 
GTGGTGTCGT TGCTTGCTGG GCTCCCTTTA CAGCCCGAGG AAGGAGATGG GGTGGAATTG 
ATGGAGGCCA AATCCCAACT GTTTCTTAAA TACTTTACAC TATTTATGAA CCTTCTTAAT 
GACTGCAGTG AGATTGAAGA AGACAATGTA CAAATTGGGG GCAGGAAGCG AGGCATGTCG 
AGAAGGCTGG CCTCCCTGAG GCACTGCACT GTGCTTGCGA TGTCCAACTT GCTCAATGCT 
AACGTTGATA GCGGCCTGAT GCACTCCATA GGATTGGGTT ATCACAAAGA CCTTCAGACA 
AGAGCTACCT TCATGGAAGT CCTCACGAAA ATCCTTCAAC AAGGCACAGA GTTTGACACA 
CTGGCAGAAA CAGCATTGGC TGATCGATTT GAGAGGCTCG TGGAATTGGT CACAATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCCTTGGCCA ATGTGGTACC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTCACT CTCTTTGATT CTCGACATTT ACTCTACCAG 
CTGCTTTGGA ACATGTTCTC TAAGGAGGTC GAGCTGGCCG ACTCCATGCA GACACTTTTT 
AGAGGGAACA GCTTGGCCAG TAAAATCATG ACCTTCTGTT TTAAGGTCTA TGGTGCTACC 
TACCTGCAGA AGCTTCTGGA GCCTTTACTG CGATTTGTGA TCACTTCCTC TGAATGGCAG 
CATGTCAGCT TTGAAGTCGA TCCCACCAGG TTAGAGCATT CAGAGAGCCT TGAGGAGAAT 
CAGCGTAATC TCTTGCAAAT GACTGAGAAG TTCTTCCATG CCATCATCAG TTCTTCCTCA 
GAGTTCCCCC CTCAGCTCCG GAGTGTGTGC CATTGCTTAT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TGTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGAT CCCTAGAATT 
GAAAGGGGAT TGAAGCTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA CGTCCTCTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GACTTTGTGA AAAGCAACTT TGATGTAGCC 
CGGAGATTTT TCCTTGATAT TGCGTCTGAT TGCCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGTGATGG CAATGTCCTT GCTCTTCACC GTCTTCTCTG GAACAACCAA 
GAGAAAATTG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAAA TGGCAACTCT CCTTGCCTAC CTGGGCCCTC CAGAACACAA GCCTGTGGCC 
GACACTCACT GGTCCAGCCT GAATCTTACC AGTTCTAAGT TTGAGGAATT TATGACAAGG 
CACCAAGTGC ACGAGAAGGA GGAGTTCAAG GCGCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAGGCTGG GAACCCCGTT TTTTATTATG TCACACGCAG GTTCAAGACT 
GGCCAGATCA ATGGGGATTT GCTGATCTAC CACGTCTTAC TAACTTTAAA GCCATATTAC 
GCGAAGCCAT ACGAGATCGT GATGGACCTC ACCCATGCCG GGCCCAGCAA TCGCTTCAAG 
ACAGACTTCC TCTCCAAGTG GTTCGTCGTG TTCCCGGGCC TCGCTTACGA GAACGTGTCC 
GCCGTCTACA TCTACAACTG CAACTCTTGG GTCCGGGAGT ACACCAAGTA CCACGAGAGG 
CTGCTCACCG GCCTCAAAGG CAGCAAGAGG CTCTTCTTCA TAGACTGCCC GGCGAAACTG 
GCGGAGCACA TCGATTACGA CCAGCAGAAG CTGCCGGCCG CCACGCTGGC CTTGGACGAG 
GACCTCAAGG TGTACAACAG CGCTCTCAAG CTGGCTCACA AAGACACCAA GGTGACGCTG 
AAGGTCGGCT CCACAGCGAT TCAAGTAACG TCTTCTGAGC GAACAAAAGT CCTGGGGCAG 
ACGGTTTTTC TGAATGATAT TTACTACGCC TCTGAGATCG AGGAAATCTG TCTAGTGGAT 
GAAAACCAGT TCACCTTAAC GATTTCCAAT CAGGGCACTC CACTCACCTT CATGCATCAA 
GAGTGCGAAG GCATTGTTGC CTCCATCATT CATATCCGAA CTAGATGGGA GCTGTCCCAG 
CCAGATTCCA TCCCCCAACA CACTAAGATC CGGCCAAAGG ATGTTCCTGG AACACTTTTG 
AATATTGCAT TACTTAATTT GGGTAGCTCG GACCCTAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACG 
TCAGGTCTGT GTATCCCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACGCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCTAGTA TTGAACTGAA ACACCTGTGT CTAGAATACA TGACTCCCTG GCTATCTAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAGCGGC AGAGAGTCAC TGCTATTCTC 
GATAAACTCA TAACAATGAC CATAAATGAA AAGCAGATGT ATCCATCTAT CCAAGCAAAG 
ATCTGGGGAA GCCTCGGGCA GATTACAGAT CTGCTTGATG TAGTACTGGA CAGCTTCATC 
AAAACCAGTG CCACAGGTGG TTTGGGTTCC ATCAAGGCTG AAGTGATGGC CGATACAGCT 
GTGGCGCTGG CTTCTGGAAA CGTGAAACTG GTATCCAGCA AGGTGATTGG AAGAATGTGC 
AAAATAATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAACAACA CCTCATGTGG 
GATGATATCG CCATTTTAGC CCGCTATATG CTAATGCTAT CCTTCAATAA TTCTCTGGAT 
GTAGCGGCTC ATCTTCCCTA CCTCTTCCAT GTTGTGACTT TGTTGGTGGC CACAGGCCCC 
CTGTCCCTTC GTGCTTCCAC TCACGGACTA GTCATCAATA TCATCCATTC TCTCTGCACT 
TGCTCCCAGC TTCATTTCAG TGAAGAGACC AAGCAAGTTT TACGACTCAG CCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTATTT GGCATCAGCA AAGTCAAATC AGCTGCTGTG 
ATAGCCTTCC GATCCAGTTA CCGGGATAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAA 
ACCTTTGCCC TGGCATCTCT GGAGACCGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATCCCAGC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCCCAGAGA 
TTTGCCTTCC AGTATAACCC CTCCCTGCAG CCAAGGGCCC TTGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATTAAG CAGATCATCC GCATTCTGAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAC AACAGTCAAG TTCTGATTGA AGCTACGGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCGCT GCACCGAGCT 
CTCTTCTGGG TCGCCGTCGC CGTGCTGCAG CTAGACGAAG TGGACCTGTA CTCGGCGGGC 
ACGGCTCTCC TCGAGCAGAA CCTGCACACC CTGGACACTC TCCTGCACAT CTTCAATGAC 
CAGAGCCCAG AAGAAGTATT CATGGAAATC CGGAAACCTC TGGAGTGGCA CTGCAAGCAA 
ATGGATCATT TTGTTGGACT CAACTTTAAC TCAAACTTTA ATTTTGCACT TGTCGGACAC 
CTGCTAAAGG GATTTAGGCA TCCTTCGCCT GTCACTGTTG CAAGAACCAT AAGGATTTTA 
CATACACTAC TAGCACTGGT TAACAAACAC AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTATTTAGC AGCCTTACTT ACAGTGTCTG AGGAGGTGCG AAGTCGCTGT 
AGCTTAAAAC ATAGGAAATC ACTCCTCCTA ACGGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAGGA AACACAACCC 
TGGTCCTCTC CAAAGGGTTC TGACAGACAT CTTGCAGCCA GTTATCCTCC TGTGGGCCAA 
ACCAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GACAGCCTTC CCAGGCCAAC 
ACTAAGAAGT TGCTTGGGAC CAGGAAAAGT TTTGATCATT TGATCACTGA CACCAAAGCT 
CCTAAAAGGC AAGACCCGGA ATCTGGAGTC ACCACACCCC CCAAGATGCG GAGAGTGGCA 
GAGAATGACT ACGATATGGA CACCCAGAGG ATCTCACCAC ACCCGCCACT ACGGAAAGTG 
TCTGTGTCCG AATCCAACGT TCTCCTGGAC GAGGAAGTGC TCACGGACCC CAAAACCCAG 
GCTCTTCTTC TCACCGTCCT GGCTACCTTG GTTAAGTACA CTGCAGATGA ATTTGACCAG 
CGCATTCTCT ATGAGTATTT GGCAGAGGCC AGTGTTGTCT TCCCAAAGGT TTTTCCAGTT 
GTACATAATT TGTTGGACTC AAAGATAAAT ACTCTGCTGT CCTTATGCCA AGACCCAAAT 
TTATTAAACC CAGTCCACGG GATTGTGCAG AGTGTGGTCT ATCACGAAGA GGCCCCGCCA 
CAATACCAGA CAGCGTACCT GCAAAGTTTT GGTTTTAATG GTCTGTGGAG GTTTGCAGGA 
CCATTTTCAA AGCAGACCCA GATCCCAGAC TACGCCGAGC TCATCGTGAA GTTCCTCGAC 
GCCTTGATCG ACACGTACCT GCCTGGGATC GATGAAGAAG CCAGTGAGGA GTCTCTCCTG 
ACCCCCACGT CCCCCTACCC TCCGGCAGTG CAGAGCCAGC TCAGCATCAC GGCCAACCTG 
AACCTCTCCA ACTCCATGAC CTCGCTGGCC ACTTCCCAGC ATTCCCCAGG CATCGACAAG 
GAGAATGTGG AGCTGTCTCC AACCCACGGG CACTCAAGCA GCGGGAGGAC TCGCCATGGC 
TCTGCCAGCC AAGTGCAGAA GCAGAGGAGC GCCGGCAGCT TCAAACGGCA CAGCATTAAG 
AAGATTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016284729.1
CDS	77..7948
Translation	MADCAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHAAIRMAPMPTSHCFAWQWASVASLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPESQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGVGYDGSSGGGTSHISLDHEEFMRACAPGPPLRKVKGNSLMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYTKWEQATKSILNYPKAKMEDSQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQHRSNVGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHSEGSSEHMGQASIEIMMLNLVRYVRVFGNLVHAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQATDEDIKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEIEEDNVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPIPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGLAYENVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDCPAKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDTLLHIFNDQSPEEVFMEIRKPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGFRHPSPVTVARTIRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSDRHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLITDTKAPKRQDPESGVTTPPKMRRVAENDYDMDTQRISPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTHGHSSSGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_016429243.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica neurofibromin 1 (NF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016429243.1

ORF Insert Sequence:

ATGGCTGATT GTGCAGAAAA ATTATTTGAC TTGGTGGATG GTTTTGCTGA AAGCACCAAA 
CGTAAAGCTG CAGTTTGGCC ACTGCAAATC ATTCTTCTTG TCTTGTGTCC AGAAATAATT 
CAGGACATTT CCAAGGATGT GGTTGATGAA GCAAAAATGA ATAAGAAAAT GTTTCTTGAC 
AATCTCAGAA GAGCGCTAGC AGGCCATGGT GGGAGCAGGC AGCTGACGGA GAGCGCAGCC 
ATCGCTTGTG TGAAGCTATG CAAAGCCAGC ACCTACATCA ACTGGGAGGA TAACTCAGTC 
ATCTTCCTGC TTGTCCAGTC CATGGTGGTA GACCTGAAGA ACTTGCTTTT TAATCCAAGC 
AAACCGTTCT CGAGAGGCAG TCAAAATGCT GACGTGGACC TCATGATTGA CTGCTTGGTT 
TCTTGCTTTC GTATAAGCCC TCACAATAAC CAACACTTTA AGATCTGTTT GGCCCAGAAC 
TCCCCATCCA CATTTCATTA TGTGCTGGTA AACTCCCTCC ATCGAATTAT CACCAATAGC 
ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCACTGGATT GGTGGCCCAA GATTGATGCT 
GTGTATTGCC ATTCTGTTGA ACTACGAAAC ATGTTTGGTG AAACACTTCA TAAGGCCGTG 
CAGGGCTGTG GGGCACATGC CGCGATACGA ATGGCACCGA TGCCAACCAG TCACTGTTTT 
GCGTGGCAGT GGGCTTCTGT GGCTAGTCTT ACATTTAAAG AAAAAATGAC AAGCCTTAAA 
TTTAAAGAAA AACCTACGGA CTTGGAAACA AGAAGCTATA AATACCTCCT ATTATCCATG 
GTGAAACTAA TCCATGCAGA CCCTAAGCTG TTGCTATGTA ATCCAAGAAA ACAAGGGCCA 
GAATCTCAGG GGAGTACAGC AGAGTTAATC ACAGGCCTTG TGCAGCTTGT CCCACAGTCT 
AGCATGCCAG AAATAGCCCA AGAAGCAATG GAGGCTTTGC TGGTTCTTCA TCAGTTGGAT 
AGCATTGACT TATGGAATCC AGATTCACCT ATAGAAACAT TCTGGGAGAT TAGCTCACAA 
ATGCTTTTTT ATATCTGTAA AAAGCTAACT ACCCATCAGA TGCTTAGCAG TACAGAAATT 
CTCAAATGGT TGCGGGAAAT TTTGATCTGT AGGAACAAAT ATCTTCTTAA AAATAAGCAG 
TCAGATAGGA GTTCTTCCCA TTTTCTCTAC CTTTATGGGG TAGGGTATGA TGGCTCTTCT 
GGTGGCGGTA CCAGTCATAT CTCTCTAGAC CATGAAGAGT TTATGCGTGC CTGTGCTCCA 
GGACCACCTC TCAGGAAAGT AAAAGGAAAC TCCTTGATGG ATGGTGCAAT AGGGTCCAGT 
GGGACGCCAA TATGTCGCCA GGCTCAGACT AAGCTGGAGG TCGCTCTGTA CATGTTTCTG 
TGGAGCCCCG ACATTGAAGC TGTGCTTGTG GCCATGTCCT GCTTTCGACA CCTCTGTGAG 
GAAGCCGATA TCAGGTGTGG CATAGATGAG ATTTCCGTGC ATCATTTCTT GCCTAACTAT 
AATACCTTCA TGGAATTTGC GTCTGTCAGC AACATGATGT CAACAGGAAG AGCAGCTCTT 
CAGAAAAGAG TGATGGCATT GCTAAGGCGC ATCGAACACC CGACTGCAGG CAACACCGAG 
GCTTGGGAAG ATACTTATAC AAAATGGGAA CAAGCTACAA AATCAATCCT CAATTATCCA 
AAAGCCAAAA TGGAAGATAG CCAGGTTGCT GAAAGTCTTC ATAAGACCAT TGTGAAAAGG 
AGGATGTCTC ATGTGAGTGG AGGAGGCTCC ATAGACTTGT GTGACACAGA CTCACTCCAA 
GAGTGGATCA ACATGACTGG TTTTCTTTGT GCCCTCGGTG GGGTGTGCCT GCAGCACCGA 
AGCAATGTTG GCTTTGCCAC CTACAGCCCT CCAATGGGCC CCATCAGTGA GCGCAAGGGC 
TCTCTGATTT CCATGGTGTC CTCAGATGGA AACGCAGAGA CACCCGTCAG CAAGTTCATG 
GATCGGCTGC TCTCCTTGAT GGTGTGTAAC CATGAAAAAG TGGGGCTGCA GATACGGAGC 
AACGTCAAGG ATTTGTTGGG CCTAGAATTG AGTCCTGCCC TTTATCCAAT GCTGTTTAAC 
AAATTAAAAA ATACCATCAG CAAGTTTTTT GATCCTCAAG GCCAGGTTTT ATTGACTGAC 
ACCAATACCC AATTTGTGGA ACAGACCATA GCCATAATGA AGAACTTGCT CGACAATCAC 
AGTGAGGGCA GTTCTGAACA CATGGGGCAA GCTAGCATCG AAATAATGAT GTTAAATCTG 
GTCAGATACG TTAGAGTGTT TGGGAATTTG GTACACGCAA TTCAGATAAA AACTAAACTC 
TGCCAGCTGG TTCAAGTGAT GATGGAGAGG AGAGATGACC TTTCATTTTG CCAGGAGATG 
AAATTTAGGA ACAAAATGGT GGAATATCTG ACAGATTGGG TCATGGGGAC ATCTAACCAA 
GCAACAGATG AAGATATAAA ATGTCTAACT AGAGATTTGG ACCAGGCAAG CATGGAAGCA 
GTGGTGTCGT TGCTTGCTGG GCTCCCTTTA CAGCCCGAGG AAGGAGATGG GGTGGAATTG 
ATGGAGGCCA AATCCCAACT GTTTCTTAAA TACTTTACAC TATTTATGAA CCTTCTTAAT 
GACTGCAGTG AGATTGAAGA AGACAATGTA CAAATTGGGG GCAGGAAGCG AGGCATGTCG 
AGAAGGCTGG CCTCCCTGAG GCACTGCACT GTGCTTGCGA TGTCCAACTT GCTCAATGCT 
AACGTTGATA GCGGCCTGAT GCACTCCATA GGATTGGGTT ATCACAAAGA CCTTCAGACA 
AGAGCTACCT TCATGGAAGT CCTCACGAAA ATCCTTCAAC AAGGCACAGA GTTTGACACA 
CTGGCAGAAA CAGCATTGGC TGATCGATTT GAGAGGCTCG TGGAATTGGT CACAATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCCTTGGCCA ATGTGGTACC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTCACT CTCTTTGATT CTCGACATTT ACTCTACCAG 
CTGCTTTGGA ACATGTTCTC TAAGGAGGTC GAGCTGGCCG ACTCCATGCA GACACTTTTT 
AGAGGGAACA GCTTGGCCAG TAAAATCATG ACCTTCTGTT TTAAGGTCTA TGGTGCTACC 
TACCTGCAGA AGCTTCTGGA GCCTTTACTG CGATTTGTGA TCACTTCCTC TGAATGGCAG 
CATGTCAGCT TTGAAGTCGA TCCCACCAGG TTAGAGCATT CAGAGAGCCT TGAGGAGAAT 
CAGCGTAATC TCTTGCAAAT GACTGAGAAG TTCTTCCATG CCATCATCAG TTCTTCCTCA 
GAGTTCCCCC CTCAGCTCCG GAGTGTGTGC CATTGCTTAT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TGTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGAT CCCTAGAATT 
GAAAGGGGAT TGAAGCTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA CGTCCTCTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GACTTTGTGA AAAGCAACTT TGATGTAGCC 
CGGAGATTTT TCCTTGATAT TGCGTCTGAT TGCCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGTGATGG CAATGTCCTT GCTCTTCACC GTCTTCTCTG GAACAACCAA 
GAGAAAATTG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAAA TGGCAACTCT CCTTGCCTAC CTGGGCCCTC CAGAACACAA GCCTGTGGCC 
GACACTCACT GGTCCAGCCT GAATCTTACC AGTTCTAAGT TTGAGGAATT TATGACAAGG 
CACCAAGTGC ACGAGAAGGA GGAGTTCAAG GCGCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAGGCTGG GAACCCCGTT TTTTATTATG TCACACGCAG GTTCAAGACT 
GGCCAGATCA ATGGGGATTT GCTGATCTAC CACGTCTTAC TAACTTTAAA GCCATATTAC 
GCGAAGCCAT ACGAGATCGT GATGGACCTC ACCCATGCCG GGCCCAGCAA TCGCTTCAAG 
ACAGACTTCC TCTCCAAGTG GTTCGTCGTG TTCCCGGGCC TCGCTTACGA GAACGTGTCC 
GCCGTCTACA TCTACAACTG CAACTCTTGG GTCCGGGAGT ACACCAAGTA CCACGAGAGG 
CTGCTCACCG GCCTCAAAGG CAGCAAGAGG CTCTTCTTCA TAGACTGCCC GGCGAAACTG 
GCGGAGCACA TCGATTACGA CCAGCAGAAG CTGCCGGCCG CCACGCTGGC CTTGGACGAG 
GACCTCAAGG TGTACAACAG CGCTCTCAAG CTGGCTCACA AAGACACCAA GGTGACGCTG 
AAGGTCGGCT CCACAGCGAT TCAAGTAACG TCTTCTGAGC GAACAAAAGT CCTGGGGCAG 
ACGGTTTTTC TGAATGATAT TTACTACGCC TCTGAGATCG AGGAAATCTG TCTAGTGGAT 
GAAAACCAGT TCACCTTAAC GATTTCCAAT CAGGGCACTC CACTCACCTT CATGCATCAA 
GAGTGCGAAG GCATTGTTGC CTCCATCATT CATATCCGAA CTAGATGGGA GCTGTCCCAG 
CCAGATTCCA TCCCCCAACA CACTAAGATC CGGCCAAAGG ATGTTCCTGG AACACTTTTG 
AATATTGCAT TACTTAATTT GGGTAGCTCG GACCCTAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACG 
TCAGGTCTGT GTATCCCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACGCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCTAGTA TTGAACTGAA ACACCTGTGT CTAGAATACA TGACTCCCTG GCTATCTAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAGCGGC AGAGAGTCAC TGCTATTCTC 
GATAAACTCA TAACAATGAC CATAAATGAA AAGCAGATGT ATCCATCTAT CCAAGCAAAG 
ATCTGGGGAA GCCTCGGGCA GATTACAGAT CTGCTTGATG TAGTACTGGA CAGCTTCATC 
AAAACCAGTG CCACAGGTGG TTTGGGTTCC ATCAAGGCTG AAGTGATGGC CGATACAGCT 
GTGGCGCTGG CTTCTGGAAA CGTGAAACTG GTATCCAGCA AGGTGATTGG AAGAATGTGC 
AAAATAATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAACAACA CCTCATGTGG 
GATGATATCG CCATTTTAGC CCGCTATATG CTAATGCTAT CCTTCAATAA TTCTCTGGAT 
GTAGCGGCTC ATCTTCCCTA CCTCTTCCAT GTTGTGACTT TGTTGGTGGC CACAGGCCCC 
CTGTCCCTTC GTGCTTCCAC TCACGGACTA GTCATCAATA TCATCCATTC TCTCTGCACT 
TGCTCCCAGC TTCATTTCAG TGAAGAGACC AAGCAAGTTT TACGACTCAG CCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTATTT GGCATCAGCA AAGTCAAATC AGCTGCTGTG 
ATAGCCTTCC GATCCAGTTA CCGGGATAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAA 
ACCTTTGCCC TGGCATCTCT GGAGACCGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATCCCAGC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCCCAGAGA 
TTTGCCTTCC AGTATAACCC CTCCCTGCAG CCAAGGGCCC TTGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATTAAG CAGATCATCC GCATTCTGAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAC AACAGTCAAG TTCTGATTGA AGCTACGGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCGCT GCACCGAGCT 
CTCTTCTGGG TCGCCGTCGC CGTGCTGCAG CTAGACGAAG TGGACCTGTA CTCGGCGGGC 
ACGGCTCTCC TCGAGCAGAA CCTGCACACC CTGGACACTC TCCTGCACAT CTTCAATGAC 
CAGAGCCCAG AAGAAGTATT CATGGAAATC CGGAAACCTC TGGAGTGGCA CTGCAAGCAA 
ATGGATCATT TTGTTGGACT CAACTTTAAC TCAAACTTTA ATTTTGCACT TGTCGGACAC 
CTGCTAAAGG GATTTAGGCA TCCTTCGCCT GTCACTGTTG CAAGAACCAT AAGGATTTTA 
CATACACTAC TAGCACTGGT TAACAAACAC AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTATTTAGC AGCCTTACTT ACAGTGTCTG AGGAGGTGCG AAGTCGCTGT 
AGCTTAAAAC ATAGGAAATC ACTCCTCCTA ACGGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAGGA AACACAACCC 
TGGTCCTCTC CAAAGGGTTC TGACAGACAT CTTGCAGCCA GTTATCCTCC TGTGGGCCAA 
ACCAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GACAGCCTTC CCAGGCCAAC 
ACTAAGAAGT TGCTTGGGAC CAGGAAAAGT TTTGATCATT TGATCACTGA CACCAAAGCT 
CCTAAAAGGC AAGACCCGGA ATCTGGAGTC ACCACACCCC CCAAGATGCG GAGAGTGGCA 
GAGAATGACT ACGATATGGA CACCCAGAGG ATCTCACCAC ACCCGCCACT ACGGAAAGTG 
TCTGTGTCCG AATCCAACGT TCTCCTGGAC GAGGAAGTGC TCACGGACCC CAAAACCCAG 
GCTCTTCTTC TCACCGTCCT GGCTACCTTG GTTAAGTACA CTGCAGATGA ATTTGACCAG 
CGCATTCTCT ATGAGTATTT GGCAGAGGCC AGTGTTGTCT TCCCAAAGGT TTTTCCAGTT 
GTACATAATT TGTTGGACTC AAAGATAAAT ACTCTGCTGT CCTTATGCCA AGACCCAAAT 
TTATTAAACC CAGTCCACGG GATTGTGCAG AGTGTGGTCT ATCACGAAGA GGCCCCGCCA 
CAATACCAGA CAGCGTACCT GCAAAGTTTT GGTTTTAATG GTCTGTGGAG GTTTGCAGGA 
CCATTTTCAA AGCAGACCCA GATCCCAGAC TACGCCGAGC TCATCGTGAA GTTCCTCGAC 
GCCTTGATCG ACACGTACCT GCCTGGGATC GATGAAGAAG CCAGTGAGGA GTCTCTCCTG 
ACCCCCACGT CCCCCTACCC TCCGGCAGTG CAGAGCCAGC TCAGCATCAC GGCCAACCTG 
AACCTCTCCA ACTCCATGAC CTCGCTGGCC ACTTCCCAGC ATTCCCCAGG CATCGACAAG 
GAGAATGTGG AGCTGTCTCC AACCCACGGG CACTCAAGCA GCGGGAGGAC TCGCCATGGC 
TCTGCCAGCC AAGTGCAGAA GCAGAGGAGC GCCGGCAGCT TCAAACGGCA CAGCATTAAG 
AAGATTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NF1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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