NWD2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NWD2
Entrez Gene ID	100022885
Full Name	NACHT and WD repeat domain containing 2
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001374552.4	XP_001374589.2	NACHT and WD repeat domain-containing protein 2

Pathways from BioSystems
Gene Ontology

The following NWD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NWD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMc25318	XM_001374552.4 Latest version!	Monodelphis domestica NACHT and WD repeat domain containing 2 (NWD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NWD2 ( XM_001374552.4 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001374589.2 Monodelphis domestica NACHT and WD repeat domain containing 2 (NWD2), mRNA.

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CloneID	OMc25318
Clone ID Related Accession (Same CDS sequence)	XM_001374552.4
Accession Version	XM_001374552.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NACHT and WD repeat domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008805.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001374552.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCGG CTGGAGCGGG CAGCAAGCTG CCCTGTGCCC GAGACTCTGC TCTCCGGCGG 
GCGGCTTTCT CCGGAAACCT CACGGCTTTG CCTTCCCATC TAGTGCCGGC CGGCAGAAGC 
GTCCGGGTCT TCATCAGCGC CAATCCTGAA GATACAGGAG CAGAACGACA AGCTCTAAGA 
GAAAATGTGT ATCCTAAACT GAGGGAATTC TGCAGAGAAA ACTATGGATT GGAATTTCAG 
GTCATAGATT TATACTGGGG CTTAGATGAA GATGAATGGG ACAGTCCTGA ACTCCAGAAG 
ACTCGTATGA AGCTTTTAGA AGATTGCTTG AAAACATCTG CAGGTCCATG TTTTGTTGGA 
CTATTGGGTG AAAAGTATGG GAACATCCGA ATTCCTGGAG AAGTTGAATC ATCAGAATTT 
GAAATGATTT TAGATGCTGC CATAGAAGCA AAGCTGGAAA CCAAGGTGCT TGAAGAATGG 
TACTGCCGGG ATGAGAACTC TGTCCCAGCA ACATATTACC TCAGACCTAA ATATGAAATG 
CTGAAAAGCA ATAAGAATCC AATGCAGGCT TCTGCCAATG CTGAGAGTGA ACAAAAATGG 
CAAGAGATAT CAGAGGAGAT AAAGAAGATT TTTAAGACAG CTGTGAAATT ACTACAGGAA 
AAGGGTAAAA TGAAGCACAG CCAAGCCAAG AGATATCTCT TTTCAGCTAT TGAAGACGAG 
TTTGACTTTG CTTTGGGAAA ACAAACACCA GCATTCCTAA AGAAATGTGT TTGCTACATT 
AGAAAAATTG CAAACATTGA ACGTTTTGTG AAAATTCCAG CAATGGGTAA ATACATGGAC 
ATAACTGGGA CAGAACCAAG GATTATCCGT GACCCAGAAG CCCACGAGAA ACTGATAAAA 
CTCAGGGATG AATTTATTCC CACCATCGTT GCATCATCTA ATCTGAGAGT CTATACTTCC 
ATTACACATT GTGACATGAA ACTAGGTTAT TCCCAAGAAG TAGAGAACCA TTATATAGAA 
GGACTTGGTA AACAGTTTTA TGAGGATATG ATTGACATCA TTCAGGCAAC TGTACAACAG 
AATTTTGACA CAGAAACCGA TACACTGTAT GATGAAATTC TTCAGCACTC ATCCTTATGT 
AAAACATATG CCTCCTTCTA TGAGTACAAA TGTGAATCTC TAAACATAGT GCACAAATAC 
CTTCTTCCCA GCAAGGCAGG GCACATTAAC CCTCTTGTCA CTTATGGAGG ACCTTGCACT 
GGGAAAACTC TTCTGTTAGC TGAAGTAGCC AAGAAGGCTT ATGGTTGGCT ACATGAAGAG 
ATGGGACCTG AATCTGACCC AGTCATAATT GTAAGATTTC TGGGAACCAC AGACATGAGT 
ACTGATCTTA AGAACCTTCT TCTAAGCATC TGTGAACAAC TGGCAGTTAA CTACCGGTGT 
CTGGTGCAAA GCTATCCTAA GAAGATTCAT GATCTTCGGG ACTTGTTTAT AAATCTCTTG 
AATGAGTCTT CATATCAGAG ACCACTGGTA ATAATATTTG ATGCTCTAGA ACAGCTTTCT 
GACATCGATG ATGCCAGAAA ACTTTGGTGG CTTCCCATTC ACCTTCCCCG TTTTGTACGG 
ATAGTACTGT CCACCCTGCC CAACAAACAT GGGATCCTGC AGAAACTGAG GTGCCTTATT 
CATGAAGAAG ACAACTACAT TGAGCTGATT CCAAGGGATA GAAAAATGTG CAGTCAGGTC 
CTAAAACACC AGTTGCTACG TGTCAAAAGA AAAGTGACAT CAGGGCAACA GATATATGTC 
AATGAAGCTT TCTCCAAGTG CACATTGCCA ATGTTTGTCA ACTTAACCTT CAGGGAGGTG 
AGAAACTGGA GATCTCACAA GGATGTGGAT GAGTCCTCCC TCTGTGTCAC AGTCCATGAA 
AGCATAGAGC AGTTATTTTG GTCCCTGGAG AAGAAATGTG GGCAGAAACT AGTATCCCGA 
GCTCTGGGTT ATATCACCAT GGCTAAAATG GGTTTGAGTG AAATGGAGTT GGAAGATGTT 
TTAGCACTGG ACAATAGTGT CATGAATGAG CTAAATGAAA ATATACGACC AAGTAACCCC 
TTGCGGGTGC CTTATATATA TGTCGCAAGG CTTAAGGAGG GCTTGAGTGG ATACCTGATA 
GAAAGGCACG TGAAAAACGT CACACTCTTG GTGTGGGCCA ATAGGCACCT CCATCTGATA 
GCACAGAAGC TTTACCTACA TAATGACAGT GACCTACGTG AAATGCATAC AATCCTGGCA 
GATTACTTTC TGGGGGTCTG GTCTGGAGGC AGGAGGAAAG CTTTTTGCCT CGAAGATCCA 
TACTTGAATG GCTGCCTTGA TCTGGAAAGC AGAAGTTTGC TGGAGGAAGA AAAGCATTTC 
TTGGAGCAGG CATCTTTTGA TAGACAGGTT CCAGATCAAC CTTGGGTGTT TCAGTGCAAT 
CCACTGGAGC CTGACATCTT TTTTGTCAAC CATCGCAAAA TGTCAGAGCT TTTACACCAC 
TTGACAAGAT GTGGGAAAAC AGATGACCTC CTCTATGGAG TCATCATGAA CTTCAGCTGG 
CTTTACACCA TGGTAAAAAT TGGTCAGTTT GACAAAGCCC TTGCAGACAT TGAAGTGGCT 
TACAATTATT CACAGGAGAA AGAACTGAAG TTTCTGGCCA GCACCCTTCG GAGCATCAAG 
AGCAAAATCA TCACATTCCC TGGCTCCCTC TCTGCTGAGC TCCAGCAGAG GCTTCTTCCA 
GTTGTCAGTT CTTTACCCAA ACTAAGACAC CTCCTTTTGG AATGTGACAA GGATGGACCT 
AAATACTGCT CCATTGTCCC CTTACATTCT TCCATGGATG TGACTTACAG CCCTGAGAGG 
CTTCCCTTGT CATCTAGTCA TCTACACGTC ACGGAGATTT TGCCTACATG TAACCCAAGT 
ACTGTCCTCA CAGCTTTGGA AAATGGCTCC ATTAGCACAT GGGATGTAGA GACCCGCCAA 
CTACTCAGGC AAATCACCAC AGCCCAGTCT GTTATTCTGG GCATGAAGCT CACCACAGAT 
GAAAAGTACC TTGTGGTAGC CACAACAAAC AACACCCTGT TGATTTATGA CAACATAAAT 
TCCTGCCTCC TCTCTGAAGT TGAAATCAAA GGAGCCAAGC ATAGCAGTGG CTCTAATTTC 
ATCAATGGGT TTACCTTGTC AATTACACAT GCCCTTGCCT GGTTGGAGGC CAGCAAAGAT 
GTCACTGTCA TTGATCTACT CTATGGATGG CCTCTTTATC AGTTCCACTG CTGGTATGAA 
GTCACTTGTG TCCAGTGCTC TCTGGATGGA GTGTACGCTT TCTGTGGGCA ATCTCTAAAT 
ACAACCACCA TCTTTCATTT AGGGAGCGGA GAAAAGCTCT CCACTGTGAC ATCTGAATTT 
TCAGGTGGCT TTGTGAAGTT TCTTCTAATC TTGGACACAG CCCAGGAAAT GGTCATGGTT 
GACAGTGAGG GCAGCCTTTC TGTTTGGAAC ACTGAGGAAA TCACCAATCC CCAGTTAACA 
GATGATTTTG ATTGTCGAAG AGAAGACAGT GAAGTTGTCA GCATTGAACT TTCTGAAGAT 
CAAAGTGCAA TTCTTATTTG CAAAGCCCTC AGCATAGAAC TCTTAGACAC TACCATGTGG 
AAGGTGGCTG AGAAATTCAG AGCCAAACAC AACGAGCGCT TTATCTCAGC GGTGCTCTCC 
AAAAATGGAG ACTGTATAAT TGCTACTATG GAAAATACCT CAGCTGTGTT TTTTTGGAGG 
CGGGACACTG GACAGTGTAT GGCGAGCTTA CAGGAAATCT CAGGAGCCAT AGTCAAGTTG 
GTCAAGTCTA ATCACCATAA CATGCTGCTA TCTCTATCTA CAAGTGGTGT CCTTTCTATT 
TGGGACATTG ATATAATTAC AGCTATGTCC AACATAGACA AGACTGGAAA ACCCATCCAA 
AGACTGATGT TACCTGCGAG AGGTGAAATC ATTTATTCCC TAGATGGATC TGAGTCTGTC 
CATAAGTGGA ACTTTAGCAA TGGATTCATT GAGGCAGAAT TTAAGCATGA AGGCATCGTG 
GAACACTGTG TGCTCACCTC CTCTGGGGAT GTGATGGTAA CATCAGATGA CAAATGTAGC 
CAATATGTAT GGCACACCAG CAGTGGTGAA AACCTCTTTC GAATTAATGG GCAAAGAATA 
TCCCAGCTGC TGATTACACA CAATGATCAA TTTGTGGTTT CACTCTGTGA GGAAAATGCC 
TCCCGGGTTT GGCGACTGGC CACAGGCCAC AGGGTGTGCA ATATTCTCAC TCCTTTACAG 
AATGCATTTA TAACCTCTGC AAATACATTT GTGGTAGGCA TTACAAAAAA CAAAGTGCTG 
GCCGTCAGTT TATGGACAGG AAGTATCACC AAGAAGTTCT GCTGTGAGGA TGGAACCATG 
ATTGTAAATT TCAAATTGAT ACCTGACTGC CCTGATATCA TAGTGTTTAT AACATCCACA 
GAAACTGTGA ACATCTGGAG CCTGACAGAC GAAGTCATCT GTCGACGTGT ACAACTTCCA 
AACAATTTCT TAAAAAATCT AGAGGACTTT GAGATTTCCC CCAATGGGAA ACTAGGTATT 
ATATCCCGGG GAGATGAAAA TATAAATGTT CTTGATCTAC ATAGTGGTAA ACTCCGAGTG 
GTCCATGCTG CTGGAATCAT CTGGCGGCAG AAGCTATCTC GAGATGGCCG CTACCTGGTC 
TACATTTGCT TCCGGAATGG AGAGGAGGAG GATGACAATG GAGCGGTCTC CAGCTTAATT 
GTCATGAGAC TCGCAGATGG CAAAAATATT GGTGCTTGCT CACTTTATAA GACGCCAACC 
TTTCTTGCAC TGTCACAAAG GCATTTAAAT ATCATTGTTG GTTTTGATGA TGGAAGTATA 
GGGATTTATA CTGTAGTGGA CAGGGTGGAT GCGGCACTGA AGATTAAAAT CGCCACCTCA 
AACAGCCGGC AAATTTTCAA CAGTGCAACA CAAGCGTCCA GGCCAAAGTG TAACAGCTAT 
TGCTTTAAGT CTACTGCAGA CTGTATTTGG AGAGAATCCA CTGAGGTATT TGCAAGAGAC 
AGTCCCATCA CAGTGACTGA TCCTGTAGAA ACAAATGAAG CAACGCCAAC CAAAAAACAC 
AATCCTTATG ACCGGGTATG CTCTGCCATA GAGAGCAGAG GCCACAGTTT TGCAGCTGAC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001374589.2
CDS	241..5466
Translation	MWPAGAGSKLPCARDSALRRAAFSGNLTALPSHLVPAGRSVRVFISANPEDTGAERQALRENVYPKLREFCRENYGLEFQVIDLYWGLDEDEWDSPELQKTRMKLLEDCLKTSAGPCFVGLLGEKYGNIRIPGEVESSEFEMILDAAIEAKLETKVLEEWYCRDENSVPATYYLRPKYEMLKSNKNPMQASANAESEQKWQEISEEIKKIFKTAVKLLQEKGKMKHSQAKRYLFSAIEDEFDFALGKQTPAFLKKCVCYIRKIANIERFVKIPAMGKYMDITGTEPRIIRDPEAHEKLIKLRDEFIPTIVASSNLRVYTSITHCDMKLGYSQEVENHYIEGLGKQFYEDMIDIIQATVQQNFDTETDTLYDEILQHSSLCKTYASFYEYKCESLNIVHKYLLPSKAGHINPLVTYGGPCTGKTLLLAEVAKKAYGWLHEEMGPESDPVIIVRFLGTTDMSTDLKNLLLSICEQLAVNYRCLVQSYPKKIHDLRDLFINLLNESSYQRPLVIIFDALEQLSDIDDARKLWWLPIHLPRFVRIVLSTLPNKHGILQKLRCLIHEEDNYIELIPRDRKMCSQVLKHQLLRVKRKVTSGQQIYVNEAFSKCTLPMFVNLTFREVRNWRSHKDVDESSLCVTVHESIEQLFWSLEKKCGQKLVSRALGYITMAKMGLSEMELEDVLALDNSVMNELNENIRPSNPLRVPYIYVARLKEGLSGYLIERHVKNVTLLVWANRHLHLIAQKLYLHNDSDLREMHTILADYFLGVWSGGRRKAFCLEDPYLNGCLDLESRSLLEEEKHFLEQASFDRQVPDQPWVFQCNPLEPDIFFVNHRKMSELLHHLTRCGKTDDLLYGVIMNFSWLYTMVKIGQFDKALADIEVAYNYSQEKELKFLASTLRSIKSKIITFPGSLSAELQQRLLPVVSSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSHLHVTEILPTCNPSTVLTALENGSISTWDVETRQLLRQITTAQSVILGMKLTTDEKYLVVATTNNTLLIYDNINSCLLSEVEIKGAKHSSGSNFINGFTLSITHALAWLEASKDVTVIDLLYGWPLYQFHCWYEVTCVQCSLDGVYAFCGQSLNTTTIFHLGSGEKLSTVTSEFSGGFVKFLLILDTAQEMVMVDSEGSLSVWNTEEITNPQLTDDFDCRREDSEVVSIELSEDQSAILICKALSIELLDTTMWKVAEKFRAKHNERFISAVLSKNGDCIIATMENTSAVFFWRRDTGQCMASLQEISGAIVKLVKSNHHNMLLSLSTSGVLSIWDIDIITAMSNIDKTGKPIQRLMLPARGEIIYSLDGSESVHKWNFSNGFIEAEFKHEGIVEHCVLTSSGDVMVTSDDKCSQYVWHTSSGENLFRINGQRISQLLITHNDQFVVSLCEENASRVWRLATGHRVCNILTPLQNAFITSANTFVVGITKNKVLAVSLWTGSITKKFCCEDGTMIVNFKLIPDCPDIIVFITSTETVNIWSLTDEVICRRVQLPNNFLKNLEDFEISPNGKLGIISRGDENINVLDLHSGKLRVVHAAGIIWRQKLSRDGRYLVYICFRNGEEEDDNGAVSSLIVMRLADGKNIGACSLYKTPTFLALSQRHLNIIVGFDDGSIGIYTVVDRVDAALKIKIATSNSRQIFNSATQASRPKCNSYCFKSTADCIWRESTEVFARDSPITVTDPVETNEATPTKKHNPYDRVCSAIESRGHSFAADN

Target ORF information:

RefSeq Version	XM_001374552.4
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NACHT and WD repeat domain containing 2 (NWD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001374552.4

ORF Insert Sequence:

ATGTGGCCGG CTGGAGCGGG CAGCAAGCTG CCCTGTGCCC GAGACTCTGC TCTCCGGCGG 
GCGGCTTTCT CCGGAAACCT CACGGCTTTG CCTTCCCATC TAGTGCCGGC CGGCAGAAGC 
GTCCGGGTCT TCATCAGCGC CAATCCTGAA GATACAGGAG CAGAACGACA AGCTCTAAGA 
GAAAATGTGT ATCCTAAACT GAGGGAATTC TGCAGAGAAA ACTATGGATT GGAATTTCAG 
GTCATAGATT TATACTGGGG CTTAGATGAA GATGAATGGG ACAGTCCTGA ACTCCAGAAG 
ACTCGTATGA AGCTTTTAGA AGATTGCTTG AAAACATCTG CAGGTCCATG TTTTGTTGGA 
CTATTGGGTG AAAAGTATGG GAACATCCGA ATTCCTGGAG AAGTTGAATC ATCAGAATTT 
GAAATGATTT TAGATGCTGC CATAGAAGCA AAGCTGGAAA CCAAGGTGCT TGAAGAATGG 
TACTGCCGGG ATGAGAACTC TGTCCCAGCA ACATATTACC TCAGACCTAA ATATGAAATG 
CTGAAAAGCA ATAAGAATCC AATGCAGGCT TCTGCCAATG CTGAGAGTGA ACAAAAATGG 
CAAGAGATAT CAGAGGAGAT AAAGAAGATT TTTAAGACAG CTGTGAAATT ACTACAGGAA 
AAGGGTAAAA TGAAGCACAG CCAAGCCAAG AGATATCTCT TTTCAGCTAT TGAAGACGAG 
TTTGACTTTG CTTTGGGAAA ACAAACACCA GCATTCCTAA AGAAATGTGT TTGCTACATT 
AGAAAAATTG CAAACATTGA ACGTTTTGTG AAAATTCCAG CAATGGGTAA ATACATGGAC 
ATAACTGGGA CAGAACCAAG GATTATCCGT GACCCAGAAG CCCACGAGAA ACTGATAAAA 
CTCAGGGATG AATTTATTCC CACCATCGTT GCATCATCTA ATCTGAGAGT CTATACTTCC 
ATTACACATT GTGACATGAA ACTAGGTTAT TCCCAAGAAG TAGAGAACCA TTATATAGAA 
GGACTTGGTA AACAGTTTTA TGAGGATATG ATTGACATCA TTCAGGCAAC TGTACAACAG 
AATTTTGACA CAGAAACCGA TACACTGTAT GATGAAATTC TTCAGCACTC ATCCTTATGT 
AAAACATATG CCTCCTTCTA TGAGTACAAA TGTGAATCTC TAAACATAGT GCACAAATAC 
CTTCTTCCCA GCAAGGCAGG GCACATTAAC CCTCTTGTCA CTTATGGAGG ACCTTGCACT 
GGGAAAACTC TTCTGTTAGC TGAAGTAGCC AAGAAGGCTT ATGGTTGGCT ACATGAAGAG 
ATGGGACCTG AATCTGACCC AGTCATAATT GTAAGATTTC TGGGAACCAC AGACATGAGT 
ACTGATCTTA AGAACCTTCT TCTAAGCATC TGTGAACAAC TGGCAGTTAA CTACCGGTGT 
CTGGTGCAAA GCTATCCTAA GAAGATTCAT GATCTTCGGG ACTTGTTTAT AAATCTCTTG 
AATGAGTCTT CATATCAGAG ACCACTGGTA ATAATATTTG ATGCTCTAGA ACAGCTTTCT 
GACATCGATG ATGCCAGAAA ACTTTGGTGG CTTCCCATTC ACCTTCCCCG TTTTGTACGG 
ATAGTACTGT CCACCCTGCC CAACAAACAT GGGATCCTGC AGAAACTGAG GTGCCTTATT 
CATGAAGAAG ACAACTACAT TGAGCTGATT CCAAGGGATA GAAAAATGTG CAGTCAGGTC 
CTAAAACACC AGTTGCTACG TGTCAAAAGA AAAGTGACAT CAGGGCAACA GATATATGTC 
AATGAAGCTT TCTCCAAGTG CACATTGCCA ATGTTTGTCA ACTTAACCTT CAGGGAGGTG 
AGAAACTGGA GATCTCACAA GGATGTGGAT GAGTCCTCCC TCTGTGTCAC AGTCCATGAA 
AGCATAGAGC AGTTATTTTG GTCCCTGGAG AAGAAATGTG GGCAGAAACT AGTATCCCGA 
GCTCTGGGTT ATATCACCAT GGCTAAAATG GGTTTGAGTG AAATGGAGTT GGAAGATGTT 
TTAGCACTGG ACAATAGTGT CATGAATGAG CTAAATGAAA ATATACGACC AAGTAACCCC 
TTGCGGGTGC CTTATATATA TGTCGCAAGG CTTAAGGAGG GCTTGAGTGG ATACCTGATA 
GAAAGGCACG TGAAAAACGT CACACTCTTG GTGTGGGCCA ATAGGCACCT CCATCTGATA 
GCACAGAAGC TTTACCTACA TAATGACAGT GACCTACGTG AAATGCATAC AATCCTGGCA 
GATTACTTTC TGGGGGTCTG GTCTGGAGGC AGGAGGAAAG CTTTTTGCCT CGAAGATCCA 
TACTTGAATG GCTGCCTTGA TCTGGAAAGC AGAAGTTTGC TGGAGGAAGA AAAGCATTTC 
TTGGAGCAGG CATCTTTTGA TAGACAGGTT CCAGATCAAC CTTGGGTGTT TCAGTGCAAT 
CCACTGGAGC CTGACATCTT TTTTGTCAAC CATCGCAAAA TGTCAGAGCT TTTACACCAC 
TTGACAAGAT GTGGGAAAAC AGATGACCTC CTCTATGGAG TCATCATGAA CTTCAGCTGG 
CTTTACACCA TGGTAAAAAT TGGTCAGTTT GACAAAGCCC TTGCAGACAT TGAAGTGGCT 
TACAATTATT CACAGGAGAA AGAACTGAAG TTTCTGGCCA GCACCCTTCG GAGCATCAAG 
AGCAAAATCA TCACATTCCC TGGCTCCCTC TCTGCTGAGC TCCAGCAGAG GCTTCTTCCA 
GTTGTCAGTT CTTTACCCAA ACTAAGACAC CTCCTTTTGG AATGTGACAA GGATGGACCT 
AAATACTGCT CCATTGTCCC CTTACATTCT TCCATGGATG TGACTTACAG CCCTGAGAGG 
CTTCCCTTGT CATCTAGTCA TCTACACGTC ACGGAGATTT TGCCTACATG TAACCCAAGT 
ACTGTCCTCA CAGCTTTGGA AAATGGCTCC ATTAGCACAT GGGATGTAGA GACCCGCCAA 
CTACTCAGGC AAATCACCAC AGCCCAGTCT GTTATTCTGG GCATGAAGCT CACCACAGAT 
GAAAAGTACC TTGTGGTAGC CACAACAAAC AACACCCTGT TGATTTATGA CAACATAAAT 
TCCTGCCTCC TCTCTGAAGT TGAAATCAAA GGAGCCAAGC ATAGCAGTGG CTCTAATTTC 
ATCAATGGGT TTACCTTGTC AATTACACAT GCCCTTGCCT GGTTGGAGGC CAGCAAAGAT 
GTCACTGTCA TTGATCTACT CTATGGATGG CCTCTTTATC AGTTCCACTG CTGGTATGAA 
GTCACTTGTG TCCAGTGCTC TCTGGATGGA GTGTACGCTT TCTGTGGGCA ATCTCTAAAT 
ACAACCACCA TCTTTCATTT AGGGAGCGGA GAAAAGCTCT CCACTGTGAC ATCTGAATTT 
TCAGGTGGCT TTGTGAAGTT TCTTCTAATC TTGGACACAG CCCAGGAAAT GGTCATGGTT 
GACAGTGAGG GCAGCCTTTC TGTTTGGAAC ACTGAGGAAA TCACCAATCC CCAGTTAACA 
GATGATTTTG ATTGTCGAAG AGAAGACAGT GAAGTTGTCA GCATTGAACT TTCTGAAGAT 
CAAAGTGCAA TTCTTATTTG CAAAGCCCTC AGCATAGAAC TCTTAGACAC TACCATGTGG 
AAGGTGGCTG AGAAATTCAG AGCCAAACAC AACGAGCGCT TTATCTCAGC GGTGCTCTCC 
AAAAATGGAG ACTGTATAAT TGCTACTATG GAAAATACCT CAGCTGTGTT TTTTTGGAGG 
CGGGACACTG GACAGTGTAT GGCGAGCTTA CAGGAAATCT CAGGAGCCAT AGTCAAGTTG 
GTCAAGTCTA ATCACCATAA CATGCTGCTA TCTCTATCTA CAAGTGGTGT CCTTTCTATT 
TGGGACATTG ATATAATTAC AGCTATGTCC AACATAGACA AGACTGGAAA ACCCATCCAA 
AGACTGATGT TACCTGCGAG AGGTGAAATC ATTTATTCCC TAGATGGATC TGAGTCTGTC 
CATAAGTGGA ACTTTAGCAA TGGATTCATT GAGGCAGAAT TTAAGCATGA AGGCATCGTG 
GAACACTGTG TGCTCACCTC CTCTGGGGAT GTGATGGTAA CATCAGATGA CAAATGTAGC 
CAATATGTAT GGCACACCAG CAGTGGTGAA AACCTCTTTC GAATTAATGG GCAAAGAATA 
TCCCAGCTGC TGATTACACA CAATGATCAA TTTGTGGTTT CACTCTGTGA GGAAAATGCC 
TCCCGGGTTT GGCGACTGGC CACAGGCCAC AGGGTGTGCA ATATTCTCAC TCCTTTACAG 
AATGCATTTA TAACCTCTGC AAATACATTT GTGGTAGGCA TTACAAAAAA CAAAGTGCTG 
GCCGTCAGTT TATGGACAGG AAGTATCACC AAGAAGTTCT GCTGTGAGGA TGGAACCATG 
ATTGTAAATT TCAAATTGAT ACCTGACTGC CCTGATATCA TAGTGTTTAT AACATCCACA 
GAAACTGTGA ACATCTGGAG CCTGACAGAC GAAGTCATCT GTCGACGTGT ACAACTTCCA 
AACAATTTCT TAAAAAATCT AGAGGACTTT GAGATTTCCC CCAATGGGAA ACTAGGTATT 
ATATCCCGGG GAGATGAAAA TATAAATGTT CTTGATCTAC ATAGTGGTAA ACTCCGAGTG 
GTCCATGCTG CTGGAATCAT CTGGCGGCAG AAGCTATCTC GAGATGGCCG CTACCTGGTC 
TACATTTGCT TCCGGAATGG AGAGGAGGAG GATGACAATG GAGCGGTCTC CAGCTTAATT 
GTCATGAGAC TCGCAGATGG CAAAAATATT GGTGCTTGCT CACTTTATAA GACGCCAACC 
TTTCTTGCAC TGTCACAAAG GCATTTAAAT ATCATTGTTG GTTTTGATGA TGGAAGTATA 
GGGATTTATA CTGTAGTGGA CAGGGTGGAT GCGGCACTGA AGATTAAAAT CGCCACCTCA 
AACAGCCGGC AAATTTTCAA CAGTGCAACA CAAGCGTCCA GGCCAAAGTG TAACAGCTAT 
TGCTTTAAGT CTACTGCAGA CTGTATTTGG AGAGAATCCA CTGAGGTATT TGCAAGAGAC 
AGTCCCATCA CAGTGACTGA TCCTGTAGAA ACAAATGAAG CAACGCCAAC CAAAAAACAC 
AATCCTTATG ACCGGGTATG CTCTGCCATA GAGAGCAGAG GCCACAGTTT TGCAGCTGAC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NWD2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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