KNTC1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	KNTC1
Entrez Gene ID	100022247
Full Name	kinetochore associated 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007489898.2	XP_007489960.1	kinetochore-associated protein 1 isoform X2
XM_007489896.2	XP_007489958.1	kinetochore-associated protein 1 isoform X1
XM_007489897.2	XP_007489959.1	kinetochore-associated protein 1 isoform X1
XM_016432424.1	XP_016287910.1	kinetochore-associated protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following KNTC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNTC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc41556	XM_016432424.1 Latest version!	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc18419	XM_007489897.2 Latest version!	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc18419	XM_007489896.2 Latest version!	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc18420	XM_007489898.2 Latest version!	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KNTC1 ( XM_016432424.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287910.1 Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMc41556
Clone ID Related Accession (Same CDS sequence)	XM_016432424.1
Accession Version	XM_016432424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	kinetochore-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAA GTAGGTCCCC TGAAGGCTCA GTGTCCATGA TAGTACTTAG GTGTCTAACA 
GAAGCCTTAC CAGAAAACAG ATTGAGTAGA TTGCTTCACA AGCACAAGTT TACAGAAGCT 
GAAAACTTTG CCATCAGTTT TGGACTGGAT GTTGAGCTTG TTTATAAGGT AAAATCAAAT 
GACCTATTGG AGAGAATGGC TTTGTGTTCA GTAGGCACTC ATGGGCCAAC AGTTTGGCAA 
CAGCTTGTGG ATGAAGCTAA AGAAAGTTTA TGTAAAATCC AGGATGACCA GTTTGTGGTG 
GATTACTGCT TAAAGGCTCA GTGGCCAACC TTCGAGACAA CTCAAGAAAT GCTGAATTAT 
GCCAAAGTCA GACTTTTGAA GAAAGAAGAT AAAATACCTA CTACCTTTTC AAATGGCTTA 
ACAGAGGTAT TAAAAGCCCG TGCAAAGTTG GCTACTTTTT ATGGAGCATT TGGACCACAA 
AAATTCAGTG GAAACAGTTG GATTGAATTT TTGAAAAATG AAGATATTCT TAAGGATATC 
TATCTTCAGC TAAAAGAGGG AAACCTTTTT TGTGCTCAGT TTCTTTGGCT TCGCCATCGG 
GCAGATTTTG AGAGCAGATT TAATGTGAAC ATGCTGGACA GTTTGCTGAG CTCAATTAGC 
ACAAGTATCC CCATGCGAGC CCTTTGTCTT TGGCTTCAGA GTGATGTGAT TCCATTTGTT 
AAAAGAATCT TACCAGAAGG ACAGGGAATC CTTGCAAAGT GGTTAGAGCA AGCAGCAAGG 
AATCTTGAAT TAACTGATAA GGCAAACTGG CCGGAGAATG GACTTGAAGT GGCACAAGTG 
TTTTTTACAT CTGAAAATCC AGATTCTTTA GGACTGATCT CTTCCTGTCA CTGCATCTCA 
CTGAAGGATT GTGAAAACAC TGAAGAAATT TGCCATTTGA GGGAGCTGGT GAATAACTTG 
CAAGAACTGA TCAAACTGTA CAGGAAATAC AACTGCAAAT TAGCTCTGTC TGAATTTGAG 
AAGGAGAACA CAACTACTAT TGTATTTCGA ATGCTTGACA AAGTTTTGGC CCCAGAACTT 
ATTCCTTCTA TCTTAGAGAA ATATGTCAAA TTGTACATGA AGGAGTATAA CCTGGAAGAG 
GAGCCACTTC TTTTGCTGTA CATAAAGGAT ATGTTGGAGA GATATAGTTG CAAGTCTGCA 
TCACTCTTTG AAACTTCCTG GGAATCCAAA GCCATGGCAA TCATAGGCTG TTTGTCTGAT 
ACAGATCTCA TCTTCGATGC TGTGCTGCAG ATCATGTATG GAGCTGTGGT GCCCTGGAGC 
ACAGCTGTAG AGCAGCTGGT TAAAAAGCAC TTGGAAATGG ACCATCCCAA AGTGAAATTG 
TTGCAGGAAA GTTACAAGTT AATGGAAATG AAGAAGCTTC TACGAGGGTA TGGAATAAGA 
GATACAAATC TTTTAAACAA GGAGATAATG AGAGTGATCA GATACATTCT TAAACAAGAT 
GGCCCAACCT CTCTTGAGGA TGCTTTAAAA GTAGCCCAAG CATATCTGTT GTCTAAAGAT 
GAAATCTACA GCTTAAGAGT CATTGACTTG ATGAGCAGGG AACAGGGTGA AGAATGCCTT 
TATCTCTTGA AATCCCTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAGAG AGTGATTATA 
TGGGCACGCT TAGTGTTACA GGAAGAGCCA GATGACTCCC AAGAGGGGAA GCTAGAGAGG 
ATATCTATAG CAAGGACAAC TGTAAATATT ATAAAAGCAC TACGTGACAT TCAGAAAGAA 
AATCCTCTGA AGATGGATGA ATGTGAAGAA AACTTAAAAC TATTTAAAAC CATTAGGAGC 
TTACAGGAAG ATTTTGAGCT CTTCCTTCCA TTTGATGACT ACAGGAATCA TTCACTGGTA 
GCTGGACTTC ACAAAGAACC CATTAACTCT CAGGAAGTTT CCCAGCCCTG GCAAAAAACT 
AGGAGGACCC CAGAGGAGAC TCCTAGTCAT TCAAAGAATG TATCTCTTCA GTCGAGTTTG 
TACAGGCAGA CTCTGTGTCT GTTGGTGTCA GAGCAAGACC TGGAGGCTGA AGTAGTGCTG 
AAGGCCTTAA ATTCAGGGAG AGTTGACGAA GCCCTCCGGC TGTGCAGGGA TTTGTTTCAA 
TGTCACTGCA ATCCCAACAC GGGAAGAGTG CTGGTTTTGG CATGTCAGAA GCTTTGTCAG 
ATGTTGGGGA CCGACACTCC AATGATTGTG CCTGACGAGT TGAACCTTCC TCTGGAGATC 
TATCAGTTGG CCTGCCAAGC AGCCACTGTC TGTAGCCCAG ATTTTTTACT AGATGCTCTG 
GAAATGTGTA AATATACTTT AACTGCCATG GAGATTTACA GACAGTGCCA GATGGAAGAT 
TGTGGAACTC TAACAAAGAC TTCCTTTGGA GCTGATAGAG ACCCCTATGA AGAATGGACA 
TACAATGACT TCTTCAGTGA AGATGGAATT GTTCTCGAAT CCCAACTGGT GCTACCAGTT 
ATCTATGAGC TGATTTCATC TCTTATACAT GAGTCTGGAG GCAAAAGATA CCCTTTGGAT 
TCTGCCAGTT TGCCATATTG CTCATTTCAT AAAGGAAAGA ATCTTCTTTT ACCAATCATC 
AACCCTGTCT TAGCTCTGCT TCAGAACCTA CAGGAATCGA GCCAGTGGGA GCTGGCTCTG 
AAATTTGTAG TAGGTTCCTT TGGCATCTGC CTTCAGCATG GAATGTCCAA CTGCATGAAT 
ATCTCCTTGA GTGAGAAGCT GTATGAAGAG AAACTGTTGA TCAGTACAAA AAACATTATT 
GTTGGCATGA AAGAAAAATC CACGAACTTG GTCAGAGAAA ATGCTGCGGC CCTGTTACAC 
AAAGTGTTTA ATTGTCGTCT AGTGGATCAT GACCTGGCCC TGGGTTACTT AACTCTTTTA 
CCGAAGAAAG ATGTCTTTGA AAATATCTGG AAAGTCATCA ATACCACATG GCAGAATTAC 
AGCAAGATTT TGGCTGTATC CCAGGTTGGA GCTCAACTGG CTTATCTCTA TAAAGAAACA 
GAAATAAGGC TGAAATTCCA AGAATTAATC ATCGATGCCC AGTGGGGCCT TCGCCTTGGG 
AAACTTGGGA TTTCTTTTCA ACCAGTGTTC AGGGAGCATT CACTAAGGAA AAAAGAACTC 
ATGAGCACAC TGGTTCAGAA TTCAGATGTG GACACAACTC TCATTCTGGA ATACTGCAGC 
ACATATGAAT TGGACAGTGA TGCAGCTCTT CAACTTTTCA TCGAAACCCT GATTCTTCAG 
CATAATCATA TTGACCCCTG TGAGGAAGAC AAGGCCATGT CTTCTCTGAA ACAGCCCCAC 
TCCAAAGTTC TGGCCAAAGC AGTTGAGATT GTGCCCTTAC TGAAAAGCAC GAGGAATTTG 
GTTGTGAGTC TTAGTGGCAT TCTGCACAAG TTGGATCCAT ATGACTATGA AATTATTGAA 
GTTGTCTTGA AAATTATGCA AAAAGCTGAT GAAAAGGTCA CCAATCTCGA TATCAATCAG 
GGCTTGAGTC TGATGAAGCA TTTGAAATCT TATAGACGAG CATCTCCTCC TGGGAATCTG 
GAACATGAGT ACATCATGAA CCATACAATG ACCATGTCCT CAGCTGCTCA GATTCGGCTG 
CCTTTTCACC TGATTTTCTT TGGGAAAGTG CAGAACTTCT GGAAGATACT TTCTGCTGAG 
CTCCGGGAGG AGTCCTTCCC AACATTCCTG TTGATTGCTA AACTAATGAA GGTTTCTTTG 
GATTCTTTGT ATGTGTCGAC AGTTAAGCAA GTCTTTGAAA AAAGCTTGAA GCCAAAGTTG 
CTGAAGCAGG TGCAGGCTAA AGGCTCAACC CCGAATAACA AGGAAATAGC TAAAACCTTC 
CAGACTTTGC AGTCCTATTT ATTATCTATA GCTAATCCTG AATGGGCTGT TGCTGTTGCC 
ATCAATCTTG CTCAGGACAT TCCAGAAGGT TGCTACAAGA TAACTATATT GAAATTTTGC 
CTTTATTTGG CCGAGAAATG GTTAAAGAAT ATTTCATCCC AGGATGAAAC TCATGAGAAA 
GCTGAGGCCT TACTGAAGAA ACTGAGGGTG CAGTACCAGA GGTCAGGCAC TGAGACTGTG 
CTCATCACAC ATGGCCTGGG CTCTCCAGAG CATCTGAGAC TGCTAGGGAA GCCAGCACAA 
CTCATTGCCA GTCTATATGA GCATGGCAGC ATCCTGCCGA GGATCCAGGG ACCTGCTGAC 
AAAGACTGTC CTGATATTCA TGCAGCAGCC AAGGAAATAG CTGAAATTAA CAACTTGGAT 
TTGGAAAAAA TATGGGATGT GCTTCTTGAA AAATGGTTAT GTCCAGCGAC ACAGACCAAT 
GAAATACCTT CAGAATTCTT TAATCTTCAA GGGGATGAAG CCCTCCAAAG AGTTCTATAT 
CTCCTCCAAA TACGTCCCAT CGATTATAGT TCAAGGATGT TATTTTTGGT TGCCACATCT 
TCTGTATCTC CTTTTACTGT TGGTCAGCTG ACATTTGCCT ATAGAAGACG AGCTCTTCAC 
TGTCTCTTAT GCTTGGCCGA CAGAGAAACC ATTGAGTCTC TCTTCAAAAA GCCTTACGAG 
GAAGTAAAAT GTTTTCTGAA ATGCATCACT TTCCTGGCCG CATTTGAAAT GTTGAACATC 
CCTCTCACAT ATGAATCATT TCACAACAGT CCTAAAGAAG GAATGATTAA GGGCCTATGG 
AAAAATCATA GCCATGAACC TATGGCTGTG AGATTGGTGA CTGAACTTAG TTTGGAATAC 
AAGGTATATG ACACTCAGCT TTGGAGTGGG CTGTTACAAA AGCTGCTTGG CTTCAACATG 
ATTAGCTATC TGAGGAATGT TTTAACAGCA ATTTCCAATA TACATTCCTT GTGGCAGATC 
CCTTACTTTG GGAGAGCCTG GCAGCGGGTC TCACAGATAC CACTCATTTC AGCCTCTTGC 
CCCCTGAATC CTACTCAGCT GTTGGCTTGT CGTGAGAGTC TGGTGGTTCT TCTAGAGTGT 
CCTGTTTCCA TGGATCTTGA TATGATTGGA GTTGCCAAGC AGTTTAGCCA ACTGGAGCTT 
CCAGCCTTTG CCTTGGCCTG CCTTCTGCTG ATGCCCCACT CAGACAAGAG AGACCAGCAG 
CTTCAGAATT TCTTGGGATG GTGTAATCCT GAGGTTATTT TACAACAAAT AGATGAACAT 
ATGAATACTG GCCAGCTAGC AGGATTTGCC TGTCAAGTGA AGGGCTTTAT TCTGAATCAC 
ATCATAAATA AGAAGCAGTT TGGGATACTG GCAAACACAA AATACTTTCC ATTGTTGAAA 
TTACAAGTGA TAAGTACCCA GAAGGTCAAG GAGTTGGTGG ACTATCTAGT CAACAATGAC 
AGTTTGGATG AAGCCTCACT CCTTATAATG GAATACTTAA AGCAGTGTGG AAAAGCAGTG 
CCACTAGATA TCACTCCTTG TGACCTAGTG AAGATGTTTC TAAGTGAATC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287910.1
CDS	6..5522
Translation	MTTSRSPEGSVSMIVLRCLTEALPENRLSRLLHKHKFTEAENFAISFGLDVELVYKVKSNDLLERMALCSVGTHGPTVWQQLVDEAKESLCKIQDDQFVVDYCLKAQWPTFETTQEMLNYAKVRLLKKEDKIPTTFSNGLTEVLKARAKLATFYGAFGPQKFSGNSWIEFLKNEDILKDIYLQLKEGNLFCAQFLWLRHRADFESRFNVNMLDSLLSSISTSIPMRALCLWLQSDVIPFVKRILPEGQGILAKWLEQAARNLELTDKANWPENGLEVAQVFFTSENPDSLGLISSCHCISLKDCENTEEICHLRELVNNLQELIKLYRKYNCKLALSEFEKENTTTIVFRMLDKVLAPELIPSILEKYVKLYMKEYNLEEEPLLLLYIKDMLERYSCKSASLFETSWESKAMAIIGCLSDTDLIFDAVLQIMYGAVVPWSTAVEQLVKKHLEMDHPKVKLLQESYKLMEMKKLLRGYGIRDTNLLNKEIMRVIRYILKQDGPTSLEDALKVAQAYLLSKDEIYSLRVIDLMSREQGEECLYLLKSLPPAEAEKTAERVIIWARLVLQEEPDDSQEGKLERISIARTTVNIIKALRDIQKENPLKMDECEENLKLFKTIRSLQEDFELFLPFDDYRNHSLVAGLHKEPINSQEVSQPWQKTRRTPEETPSHSKNVSLQSSLYRQTLCLLVSEQDLEAEVVLKALNSGRVDEALRLCRDLFQCHCNPNTGRVLVLACQKLCQMLGTDTPMIVPDELNLPLEIYQLACQAATVCSPDFLLDALEMCKYTLTAMEIYRQCQMEDCGTLTKTSFGADRDPYEEWTYNDFFSEDGIVLESQLVLPVIYELISSLIHESGGKRYPLDSASLPYCSFHKGKNLLLPIINPVLALLQNLQESSQWELALKFVVGSFGICLQHGMSNCMNISLSEKLYEEKLLISTKNIIVGMKEKSTNLVRENAAALLHKVFNCRLVDHDLALGYLTLLPKKDVFENIWKVINTTWQNYSKILAVSQVGAQLAYLYKETEIRLKFQELIIDAQWGLRLGKLGISFQPVFREHSLRKKELMSTLVQNSDVDTTLILEYCSTYELDSDAALQLFIETLILQHNHIDPCEEDKAMSSLKQPHSKVLAKAVEIVPLLKSTRNLVVSLSGILHKLDPYDYEIIEVVLKIMQKADEKVTNLDINQGLSLMKHLKSYRRASPPGNLEHEYIMNHTMTMSSAAQIRLPFHLIFFGKVQNFWKILSAELREESFPTFLLIAKLMKVSLDSLYVSTVKQVFEKSLKPKLLKQVQAKGSTPNNKEIAKTFQTLQSYLLSIANPEWAVAVAINLAQDIPEGCYKITILKFCLYLAEKWLKNISSQDETHEKAEALLKKLRVQYQRSGTETVLITHGLGSPEHLRLLGKPAQLIASLYEHGSILPRIQGPADKDCPDIHAAAKEIAEINNLDLEKIWDVLLEKWLCPATQTNEIPSEFFNLQGDEALQRVLYLLQIRPIDYSSRMLFLVATSSVSPFTVGQLTFAYRRRALHCLLCLADRETIESLFKKPYEEVKCFLKCITFLAAFEMLNIPLTYESFHNSPKEGMIKGLWKNHSHEPMAVRLVTELSLEYKVYDTQLWSGLLQKLLGFNMISYLRNVLTAISNIHSLWQIPYFGRAWQRVSQIPLISASCPLNPTQLLACRESLVVLLECPVSMDLDMIGVAKQFSQLELPAFALACLLLMPHSDKRDQQLQNFLGWCNPEVILQQIDEHMNTGQLAGFACQVKGFILNHIINKKQFGILANTKYFPLLKLQVISTQKVKELVDYLVNNDSLDEASLLIMEYLKQCGKAVPLDITPCDLVKMFLSESK

Target ORF information:

RefSeq Version	XM_016432424.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016432424.1

ORF Insert Sequence:

ATGACCACAA GTAGGTCCCC TGAAGGCTCA GTGTCCATGA TAGTACTTAG GTGTCTAACA 
GAAGCCTTAC CAGAAAACAG ATTGAGTAGA TTGCTTCACA AGCACAAGTT TACAGAAGCT 
GAAAACTTTG CCATCAGTTT TGGACTGGAT GTTGAGCTTG TTTATAAGGT AAAATCAAAT 
GACCTATTGG AGAGAATGGC TTTGTGTTCA GTAGGCACTC ATGGGCCAAC AGTTTGGCAA 
CAGCTTGTGG ATGAAGCTAA AGAAAGTTTA TGTAAAATCC AGGATGACCA GTTTGTGGTG 
GATTACTGCT TAAAGGCTCA GTGGCCAACC TTCGAGACAA CTCAAGAAAT GCTGAATTAT 
GCCAAAGTCA GACTTTTGAA GAAAGAAGAT AAAATACCTA CTACCTTTTC AAATGGCTTA 
ACAGAGGTAT TAAAAGCCCG TGCAAAGTTG GCTACTTTTT ATGGAGCATT TGGACCACAA 
AAATTCAGTG GAAACAGTTG GATTGAATTT TTGAAAAATG AAGATATTCT TAAGGATATC 
TATCTTCAGC TAAAAGAGGG AAACCTTTTT TGTGCTCAGT TTCTTTGGCT TCGCCATCGG 
GCAGATTTTG AGAGCAGATT TAATGTGAAC ATGCTGGACA GTTTGCTGAG CTCAATTAGC 
ACAAGTATCC CCATGCGAGC CCTTTGTCTT TGGCTTCAGA GTGATGTGAT TCCATTTGTT 
AAAAGAATCT TACCAGAAGG ACAGGGAATC CTTGCAAAGT GGTTAGAGCA AGCAGCAAGG 
AATCTTGAAT TAACTGATAA GGCAAACTGG CCGGAGAATG GACTTGAAGT GGCACAAGTG 
TTTTTTACAT CTGAAAATCC AGATTCTTTA GGACTGATCT CTTCCTGTCA CTGCATCTCA 
CTGAAGGATT GTGAAAACAC TGAAGAAATT TGCCATTTGA GGGAGCTGGT GAATAACTTG 
CAAGAACTGA TCAAACTGTA CAGGAAATAC AACTGCAAAT TAGCTCTGTC TGAATTTGAG 
AAGGAGAACA CAACTACTAT TGTATTTCGA ATGCTTGACA AAGTTTTGGC CCCAGAACTT 
ATTCCTTCTA TCTTAGAGAA ATATGTCAAA TTGTACATGA AGGAGTATAA CCTGGAAGAG 
GAGCCACTTC TTTTGCTGTA CATAAAGGAT ATGTTGGAGA GATATAGTTG CAAGTCTGCA 
TCACTCTTTG AAACTTCCTG GGAATCCAAA GCCATGGCAA TCATAGGCTG TTTGTCTGAT 
ACAGATCTCA TCTTCGATGC TGTGCTGCAG ATCATGTATG GAGCTGTGGT GCCCTGGAGC 
ACAGCTGTAG AGCAGCTGGT TAAAAAGCAC TTGGAAATGG ACCATCCCAA AGTGAAATTG 
TTGCAGGAAA GTTACAAGTT AATGGAAATG AAGAAGCTTC TACGAGGGTA TGGAATAAGA 
GATACAAATC TTTTAAACAA GGAGATAATG AGAGTGATCA GATACATTCT TAAACAAGAT 
GGCCCAACCT CTCTTGAGGA TGCTTTAAAA GTAGCCCAAG CATATCTGTT GTCTAAAGAT 
GAAATCTACA GCTTAAGAGT CATTGACTTG ATGAGCAGGG AACAGGGTGA AGAATGCCTT 
TATCTCTTGA AATCCCTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAGAG AGTGATTATA 
TGGGCACGCT TAGTGTTACA GGAAGAGCCA GATGACTCCC AAGAGGGGAA GCTAGAGAGG 
ATATCTATAG CAAGGACAAC TGTAAATATT ATAAAAGCAC TACGTGACAT TCAGAAAGAA 
AATCCTCTGA AGATGGATGA ATGTGAAGAA AACTTAAAAC TATTTAAAAC CATTAGGAGC 
TTACAGGAAG ATTTTGAGCT CTTCCTTCCA TTTGATGACT ACAGGAATCA TTCACTGGTA 
GCTGGACTTC ACAAAGAACC CATTAACTCT CAGGAAGTTT CCCAGCCCTG GCAAAAAACT 
AGGAGGACCC CAGAGGAGAC TCCTAGTCAT TCAAAGAATG TATCTCTTCA GTCGAGTTTG 
TACAGGCAGA CTCTGTGTCT GTTGGTGTCA GAGCAAGACC TGGAGGCTGA AGTAGTGCTG 
AAGGCCTTAA ATTCAGGGAG AGTTGACGAA GCCCTCCGGC TGTGCAGGGA TTTGTTTCAA 
TGTCACTGCA ATCCCAACAC GGGAAGAGTG CTGGTTTTGG CATGTCAGAA GCTTTGTCAG 
ATGTTGGGGA CCGACACTCC AATGATTGTG CCTGACGAGT TGAACCTTCC TCTGGAGATC 
TATCAGTTGG CCTGCCAAGC AGCCACTGTC TGTAGCCCAG ATTTTTTACT AGATGCTCTG 
GAAATGTGTA AATATACTTT AACTGCCATG GAGATTTACA GACAGTGCCA GATGGAAGAT 
TGTGGAACTC TAACAAAGAC TTCCTTTGGA GCTGATAGAG ACCCCTATGA AGAATGGACA 
TACAATGACT TCTTCAGTGA AGATGGAATT GTTCTCGAAT CCCAACTGGT GCTACCAGTT 
ATCTATGAGC TGATTTCATC TCTTATACAT GAGTCTGGAG GCAAAAGATA CCCTTTGGAT 
TCTGCCAGTT TGCCATATTG CTCATTTCAT AAAGGAAAGA ATCTTCTTTT ACCAATCATC 
AACCCTGTCT TAGCTCTGCT TCAGAACCTA CAGGAATCGA GCCAGTGGGA GCTGGCTCTG 
AAATTTGTAG TAGGTTCCTT TGGCATCTGC CTTCAGCATG GAATGTCCAA CTGCATGAAT 
ATCTCCTTGA GTGAGAAGCT GTATGAAGAG AAACTGTTGA TCAGTACAAA AAACATTATT 
GTTGGCATGA AAGAAAAATC CACGAACTTG GTCAGAGAAA ATGCTGCGGC CCTGTTACAC 
AAAGTGTTTA ATTGTCGTCT AGTGGATCAT GACCTGGCCC TGGGTTACTT AACTCTTTTA 
CCGAAGAAAG ATGTCTTTGA AAATATCTGG AAAGTCATCA ATACCACATG GCAGAATTAC 
AGCAAGATTT TGGCTGTATC CCAGGTTGGA GCTCAACTGG CTTATCTCTA TAAAGAAACA 
GAAATAAGGC TGAAATTCCA AGAATTAATC ATCGATGCCC AGTGGGGCCT TCGCCTTGGG 
AAACTTGGGA TTTCTTTTCA ACCAGTGTTC AGGGAGCATT CACTAAGGAA AAAAGAACTC 
ATGAGCACAC TGGTTCAGAA TTCAGATGTG GACACAACTC TCATTCTGGA ATACTGCAGC 
ACATATGAAT TGGACAGTGA TGCAGCTCTT CAACTTTTCA TCGAAACCCT GATTCTTCAG 
CATAATCATA TTGACCCCTG TGAGGAAGAC AAGGCCATGT CTTCTCTGAA ACAGCCCCAC 
TCCAAAGTTC TGGCCAAAGC AGTTGAGATT GTGCCCTTAC TGAAAAGCAC GAGGAATTTG 
GTTGTGAGTC TTAGTGGCAT TCTGCACAAG TTGGATCCAT ATGACTATGA AATTATTGAA 
GTTGTCTTGA AAATTATGCA AAAAGCTGAT GAAAAGGTCA CCAATCTCGA TATCAATCAG 
GGCTTGAGTC TGATGAAGCA TTTGAAATCT TATAGACGAG CATCTCCTCC TGGGAATCTG 
GAACATGAGT ACATCATGAA CCATACAATG ACCATGTCCT CAGCTGCTCA GATTCGGCTG 
CCTTTTCACC TGATTTTCTT TGGGAAAGTG CAGAACTTCT GGAAGATACT TTCTGCTGAG 
CTCCGGGAGG AGTCCTTCCC AACATTCCTG TTGATTGCTA AACTAATGAA GGTTTCTTTG 
GATTCTTTGT ATGTGTCGAC AGTTAAGCAA GTCTTTGAAA AAAGCTTGAA GCCAAAGTTG 
CTGAAGCAGG TGCAGGCTAA AGGCTCAACC CCGAATAACA AGGAAATAGC TAAAACCTTC 
CAGACTTTGC AGTCCTATTT ATTATCTATA GCTAATCCTG AATGGGCTGT TGCTGTTGCC 
ATCAATCTTG CTCAGGACAT TCCAGAAGGT TGCTACAAGA TAACTATATT GAAATTTTGC 
CTTTATTTGG CCGAGAAATG GTTAAAGAAT ATTTCATCCC AGGATGAAAC TCATGAGAAA 
GCTGAGGCCT TACTGAAGAA ACTGAGGGTG CAGTACCAGA GGTCAGGCAC TGAGACTGTG 
CTCATCACAC ATGGCCTGGG CTCTCCAGAG CATCTGAGAC TGCTAGGGAA GCCAGCACAA 
CTCATTGCCA GTCTATATGA GCATGGCAGC ATCCTGCCGA GGATCCAGGG ACCTGCTGAC 
AAAGACTGTC CTGATATTCA TGCAGCAGCC AAGGAAATAG CTGAAATTAA CAACTTGGAT 
TTGGAAAAAA TATGGGATGT GCTTCTTGAA AAATGGTTAT GTCCAGCGAC ACAGACCAAT 
GAAATACCTT CAGAATTCTT TAATCTTCAA GGGGATGAAG CCCTCCAAAG AGTTCTATAT 
CTCCTCCAAA TACGTCCCAT CGATTATAGT TCAAGGATGT TATTTTTGGT TGCCACATCT 
TCTGTATCTC CTTTTACTGT TGGTCAGCTG ACATTTGCCT ATAGAAGACG AGCTCTTCAC 
TGTCTCTTAT GCTTGGCCGA CAGAGAAACC ATTGAGTCTC TCTTCAAAAA GCCTTACGAG 
GAAGTAAAAT GTTTTCTGAA ATGCATCACT TTCCTGGCCG CATTTGAAAT GTTGAACATC 
CCTCTCACAT ATGAATCATT TCACAACAGT CCTAAAGAAG GAATGATTAA GGGCCTATGG 
AAAAATCATA GCCATGAACC TATGGCTGTG AGATTGGTGA CTGAACTTAG TTTGGAATAC 
AAGGTATATG ACACTCAGCT TTGGAGTGGG CTGTTACAAA AGCTGCTTGG CTTCAACATG 
ATTAGCTATC TGAGGAATGT TTTAACAGCA ATTTCCAATA TACATTCCTT GTGGCAGATC 
CCTTACTTTG GGAGAGCCTG GCAGCGGGTC TCACAGATAC CACTCATTTC AGCCTCTTGC 
CCCCTGAATC CTACTCAGCT GTTGGCTTGT CGTGAGAGTC TGGTGGTTCT TCTAGAGTGT 
CCTGTTTCCA TGGATCTTGA TATGATTGGA GTTGCCAAGC AGTTTAGCCA ACTGGAGCTT 
CCAGCCTTTG CCTTGGCCTG CCTTCTGCTG ATGCCCCACT CAGACAAGAG AGACCAGCAG 
CTTCAGAATT TCTTGGGATG GTGTAATCCT GAGGTTATTT TACAACAAAT AGATGAACAT 
ATGAATACTG GCCAGCTAGC AGGATTTGCC TGTCAAGTGA AGGGCTTTAT TCTGAATCAC 
ATCATAAATA AGAAGCAGTT TGGGATACTG GCAAACACAA AATACTTTCC ATTGTTGAAA 
TTACAAGTGA TAAGTACCCA GAAGGTCAAG GAGTTGGTGG ACTATCTAGT CAACAATGAC 
AGTTTGGATG AAGCCTCACT CCTTATAATG GAATACTTAA AGCAGTGTGG AAAAGCAGTG 
CCACTAGATA TCACTCCTTG TGACCTAGTG AAGATGTTTC TAAGTGAATC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_007489896.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007489958.1 Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

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CloneID	OMc18419
Clone ID Related Accession (Same CDS sequence)	XM_007489896.2 , XM_007489897.2
Accession Version	XM_007489896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007489896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGAAGG ATTGTGAAAA CACTGAAGAA 
ATTTGCCATT TGAGGGAGCT GGTGAATAAC TTGCAAGAAC TGATCAAACT GTACAGGAAA 
TACAACTGCA AATTAGCTCT GTCTGAATTT GAGAAGGAGA ACACAACTAC TATTGTATTT 
CGAATGCTTG ACAAAGTTTT GGCCCCAGAA CTTATTCCTT CTATCTTAGA GAAATATGTC 
AAATTGTACA TGAAGGAGTA TAACCTGGAA GAGGAGCCAC TTCTTTTGCT GTACATAAAG 
GATATGTTGG AGAGATATAG TTGCAAGTCT GCATCACTCT TTGAAACTTC CTGGGAATCC 
AAAGCCATGG CAATCATAGG CTGTTTGTCT GATACAGATC TCATCTTCGA TGCTGTGCTG 
CAGATCATGT ATGGAGCTGT GGTGCCCTGG AGCACAGCTG TAGAGCAGCT GGTTAAAAAG 
CACTTGGAAA TGGACCATCC CAAAGTGAAA TTGTTGCAGG AAAGTTACAA GTTAATGGAA 
ATGAAGAAGC TTCTACGAGG GTATGGAATA AGAGATACAA ATCTTTTAAA CAAGGAGATA 
ATGAGAGTGA TCAGATACAT TCTTAAACAA GATGGCCCAA CCTCTCTTGA GGATGCTTTA 
AAAGTAGCCC AAGCATATCT GTTGTCTAAA GATGAAATCT ACAGCTTAAG AGTCATTGAC 
TTGATGAGCA GGGAACAGGG TGAAGAATGC CTTTATCTCT TGAAATCCCT GCCTCCTGCT 
GAAGCTGAGA AAACTGCAGA GAGAGTGATT ATATGGGCAC GCTTAGTGTT ACAGGAAGAG 
CCAGATGACT CCCAAGAGGG GAAGCTAGAG AGGATATCTA TAGCAAGGAC AACTGTAAAT 
ATTATAAAAG CACTACGTGA CATTCAGAAA GAAAATCCTC TGAAGATGGA TGAATGTGAA 
GAAAACTTAA AACTATTTAA AACCATTAGG AGCTTACAGG AAGATTTTGA GCTCTTCCTT 
CCATTTGATG ACTACAGGAA TCATTCACTG GTAGCTGGAC TTCACAAAGA ACCCATTAAC 
TCTCAGGAAG TTTCCCAGCC CTGGCAAAAA ACTAGGAGGA CCCCAGAGGA GACTCCTAGT 
CATTCAAAGA ATGTATCTCT TCAGTCGAGT TTGTACAGGC AGACTCTGTG TCTGTTGGTG 
TCAGAGCAAG ACCTGGAGGC TGAAGTAGTG CTGAAGGCCT TAAATTCAGG GAGAGTTGAC 
GAAGCCCTCC GGCTGTGCAG GGATTTGTTT CAATGTCACT GCAATCCCAA CACGGGAAGA 
GTGCTGGTTT TGGCATGTCA GAAGCTTTGT CAGATGTTGG GGACCGACAC TCCAATGATT 
GTGCCTGACG AGTTGAACCT TCCTCTGGAG ATCTATCAGT TGGCCTGCCA AGCAGCCACT 
GTCTGTAGCC CAGATTTTTT ACTAGATGCT CTGGAAATGT GTAAATATAC TTTAACTGCC 
ATGGAGATTT ACAGACAGTG CCAGATGGAA GATTGTGGAA CTCTAACAAA GACTTCCTTT 
GGAGCTGATA GAGACCCCTA TGAAGAATGG ACATACAATG ACTTCTTCAG TGAAGATGGA 
ATTGTTCTCG AATCCCAACT GGTGCTACCA GTTATCTATG AGCTGATTTC ATCTCTTATA 
CATGAGTCTG GAGGCAAAAG ATACCCTTTG GATTCTGCCA GTTTGCCATA TTGCTCATTT 
CATAAAGGAA AGAATCTTCT TTTACCAATC ATCAACCCTG TCTTAGCTCT GCTTCAGAAC 
CTACAGGAAT CGAGCCAGTG GGAGCTGGCT CTGAAATTTG TAGTAGGTTC CTTTGGCATC 
TGCCTTCAGC ATGGAATGTC CAACTGCATG AATATCTCCT TGAGTGAGAA GCTGTATGAA 
GAGAAACTGT TGATCAGTAC AAAAAACATT ATTGTTGGCA TGAAAGAAAA ATCCACGAAC 
TTGGTCAGAG AAAATGCTGC GGCCCTGTTA CACAAAGTGT TTAATTGTCG TCTAGTGGAT 
CATGACCTGG CCCTGGGTTA CTTAACTCTT TTACCGAAGA AAGATGTCTT TGAAAATATC 
TGGAAAGTCA TCAATACCAC ATGGCAGAAT TACAGCAAGA TTTTGGCTGT ATCCCAGGTT 
GGAGCTCAAC TGGCTTATCT CTATAAAGAA ACAGAAATAA GGCTGAAATT CCAAGAATTA 
ATCATCGATG CCCAGTGGGG CCTTCGCCTT GGGAAACTTG GGATTTCTTT TCAACCAGTG 
TTCAGGGAGC ATTCACTAAG GAAAAAAGAA CTCATGAGCA CACTGGTTCA GAATTCAGAT 
GTGGACACAA CTCTCATTCT GGAATACTGC AGCACATATG AATTGGACAG TGATGCAGCT 
CTTCAACTTT TCATCGAAAC CCTGATTCTT CAGCATAATC ATATTGACCC CTGTGAGGAA 
GACAAGGCCA TGTCTTCTCT GAAACAGCCC CACTCCAAAG TTCTGGCCAA AGCAGTTGAG 
ATTGTGCCCT TACTGAAAAG CACGAGGAAT TTGGTTGTGA GTCTTAGTGG CATTCTGCAC 
AAGTTGGATC CATATGACTA TGAAATTATT GAAGTTGTCT TGAAAATTAT GCAAAAAGCT 
GATGAAAAGG TCACCAATCT CGATATCAAT CAGGGCTTGA GTCTGATGAA GCATTTGAAA 
TCTTATAGAC GAGCATCTCC TCCTGGGAAT CTGGAACATG AGTACATCAT GAACCATACA 
ATGACCATGT CCTCAGCTGC TCAGATTCGG CTGCCTTTTC ACCTGATTTT CTTTGGGAAA 
GTGCAGAACT TCTGGAAGAT ACTTTCTGCT GAGCTCCGGG AGGAGTCCTT CCCAACATTC 
CTGTTGATTG CTAAACTAAT GAAGGTTTCT TTGGATTCTT TGTATGTGTC GACAGTTAAG 
CAAGTCTTTG AAAAAAGCTT GAAGCCAAAG TTGCTGAAGC AGGTGCAGGC TAAAGGCTCA 
ACCCCGAATA ACAAGGAAAT AGCTAAAACC TTCCAGACTT TGCAGTCCTA TTTATTATCT 
ATAGCTAATC CTGAATGGGC TGTTGCTGTT GCCATCAATC TTGCTCAGGA CATTCCAGAA 
GGTTGCTACA AGATAACTAT ATTGAAATTT TGCCTTTATT TGGCCGAGAA ATGGTTAAAG 
AATATTTCAT CCCAGGATGA AACTCATGAG AAAGCTGAGG CCTTACTGAA GAAACTGAGG 
GTGCAGTACC AGAGGTCAGG CACTGAGACT GTGCTCATCA CACATGGCCT GGGCTCTCCA 
GAGCATCTGA GACTGCTAGG GAAGCCAGCA CAACTCATTG CCAGTCTATA TGAGCATGGC 
AGCATCCTGC CGAGGATCCA GGGACCTGCT GACAAAGACT GTCCTGATAT TCATGCAGCA 
GCCAAGGAAA TAGCTGAAAT TAACAACTTG GATTTGGAAA AAATATGGGA TGTGCTTCTT 
GAAAAATGGT TATGTCCAGC GACACAGACC AATGAAATAC CTTCAGAATT CTTTAATCTT 
CAAGGGGATG AAGCCCTCCA AAGAGTTCTA TATCTCCTCC AAATACGTCC CATCGATTAT 
AGTTCAAGGA TGTTATTTTT GGTTGCCACA TCTTCTGTAT CTCCTTTTAC TGTTGGTCAG 
CTGACATTTG CCTATAGAAG ACGAGCTCTT CACTGTCTCT TATGCTTGGC CGACAGAGAA 
ACCATTGAGT CTCTCTTCAA AAAGCCTTAC GAGGAAGTAA AATGTTTTCT GAAATGCATC 
ACTTTCCTGG CCGCATTTGA AATGTTGAAC ATCCCTCTCA CATATGAATC ATTTCACAAC 
AGTCCTAAAG AAGGAATGAT TAAGGGCCTA TGGAAAAATC ATAGCCATGA ACCTATGGCT 
GTGAGATTGG TGACTGAACT TAGTTTGGAA TACAAGGTAT ATGACACTCA GCTTTGGAGT 
GGGCTGTTAC AAAAGCTGCT TGGCTTCAAC ATGATTAGCT ATCTGAGGAA TGTTTTAACA 
GCAATTTCCA ATATACATTC CTTGTGGCAG ATCCCTTACT TTGGGAGAGC CTGGCAGCGG 
GTCTCACAGA TACCACTCAT TTCAGCCTCT TGCCCCCTGA ATCCTACTCA GCTGTTGGCT 
TGTCGTGAGA GTCTGGTGGT TCTTCTAGAG TGTCCTGTTT CCATGGATCT TGATATGATT 
GGAGTTGCCA AGCAGTTTAG CCAACTGGAG CTTCCAGCCT TTGCCTTGGC CTGCCTTCTG 
CTGATGCCCC ACTCAGACAA GAGAGACCAG CAGCTTCAGA ATTTCTTGGG ATGGTGTAAT 
CCTGAGGTTA TTTTACAACA AATAGATGAA CATATGAATA CTGGCCAGCT AGCAGGATTT 
GCCTGTCAAG TGAAGGGCTT TATTCTGAAT CACATCATAA ATAAGAAGCA GTTTGGGATA 
CTGGCAAACA CAAAATACTT TCCATTGTTG AAATTACAAG TGATAAGTAC CCAGAAGGTC 
AAGGAGTTGG TGGACTATCT AGTCAACAAT GACAGTTTGG ATGAAGCCTC ACTCCTTATA 
ATGGAATACT TAAAGCAGTG TGGAAAAGCA GTGCCACTAG ATATCACTCC TTGTGACCTA 
GTGAAGATGT TTCTAAGTGA ATCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007489958.1
CDS	402..7031
Translation	MWDDIELLTSDDTGSGCLSIGSGEEHGTTLYQVDTLVKISSEKASFSPKLHACSSSDGFVIVVDQSVTLFDNICRSLQLHLQFDTKVDVVGLCQGGEFLLVGERSGNLHLIHVTSNQTLLTNAFVQQAPDENQCTYQDLIIEEGGAKDGKYHVFLLTYNGLFCIMDLQLTQIQEAIEKKDFIAAKELQGQTKTSFITTENYHDRGCLSFVIGDLTSENQVIIGGTGDFVLSKWELDPENKVAMVKNFIDSDMIKGAKKIQLADNLLFILDTMNVLSLWDIYTLIPVWNWPCTHFEDFILTTEADSSSSVTWEGVINLKLIALTTSSNQMRNLMVYSLPTMTLLYSLEVSAVSSLVRMGISSDTIYLLEGIYENDHKSPEGSVSMIVLRCLTEALPENRLSRLLHKHKFTEAENFAISFGLDVELVYKVKSNDLLERMALCSVGTHGPTVWQQLVDEAKESLCKIQDDQFVVDYCLKAQWPTFETTQEMLNYAKVRLLKKEDKIPTTFSNGLTEVLKARAKLATFYGAFGPQKFSGNSWIEFLKNEDILKDIYLQLKEGNLFCAQFLWLRHRADFESRFNVNMLDSLLSSISTSIPMRALCLWLQSDVIPFVKRILPEGQGILAKWLEQAARNLELTDKANWPENGLEVAQVFFTSENPDSLGLISSCHCISLKDCENTEEICHLRELVNNLQELIKLYRKYNCKLALSEFEKENTTTIVFRMLDKVLAPELIPSILEKYVKLYMKEYNLEEEPLLLLYIKDMLERYSCKSASLFETSWESKAMAIIGCLSDTDLIFDAVLQIMYGAVVPWSTAVEQLVKKHLEMDHPKVKLLQESYKLMEMKKLLRGYGIRDTNLLNKEIMRVIRYILKQDGPTSLEDALKVAQAYLLSKDEIYSLRVIDLMSREQGEECLYLLKSLPPAEAEKTAERVIIWARLVLQEEPDDSQEGKLERISIARTTVNIIKALRDIQKENPLKMDECEENLKLFKTIRSLQEDFELFLPFDDYRNHSLVAGLHKEPINSQEVSQPWQKTRRTPEETPSHSKNVSLQSSLYRQTLCLLVSEQDLEAEVVLKALNSGRVDEALRLCRDLFQCHCNPNTGRVLVLACQKLCQMLGTDTPMIVPDELNLPLEIYQLACQAATVCSPDFLLDALEMCKYTLTAMEIYRQCQMEDCGTLTKTSFGADRDPYEEWTYNDFFSEDGIVLESQLVLPVIYELISSLIHESGGKRYPLDSASLPYCSFHKGKNLLLPIINPVLALLQNLQESSQWELALKFVVGSFGICLQHGMSNCMNISLSEKLYEEKLLISTKNIIVGMKEKSTNLVRENAAALLHKVFNCRLVDHDLALGYLTLLPKKDVFENIWKVINTTWQNYSKILAVSQVGAQLAYLYKETEIRLKFQELIIDAQWGLRLGKLGISFQPVFREHSLRKKELMSTLVQNSDVDTTLILEYCSTYELDSDAALQLFIETLILQHNHIDPCEEDKAMSSLKQPHSKVLAKAVEIVPLLKSTRNLVVSLSGILHKLDPYDYEIIEVVLKIMQKADEKVTNLDINQGLSLMKHLKSYRRASPPGNLEHEYIMNHTMTMSSAAQIRLPFHLIFFGKVQNFWKILSAELREESFPTFLLIAKLMKVSLDSLYVSTVKQVFEKSLKPKLLKQVQAKGSTPNNKEIAKTFQTLQSYLLSIANPEWAVAVAINLAQDIPEGCYKITILKFCLYLAEKWLKNISSQDETHEKAEALLKKLRVQYQRSGTETVLITHGLGSPEHLRLLGKPAQLIASLYEHGSILPRIQGPADKDCPDIHAAAKEIAEINNLDLEKIWDVLLEKWLCPATQTNEIPSEFFNLQGDEALQRVLYLLQIRPIDYSSRMLFLVATSSVSPFTVGQLTFAYRRRALHCLLCLADRETIESLFKKPYEEVKCFLKCITFLAAFEMLNIPLTYESFHNSPKEGMIKGLWKNHSHEPMAVRLVTELSLEYKVYDTQLWSGLLQKLLGFNMISYLRNVLTAISNIHSLWQIPYFGRAWQRVSQIPLISASCPLNPTQLLACRESLVVLLECPVSMDLDMIGVAKQFSQLELPAFALACLLLMPHSDKRDQQLQNFLGWCNPEVILQQIDEHMNTGQLAGFACQVKGFILNHIINKKQFGILANTKYFPLLKLQVISTQKVKELVDYLVNNDSLDEASLLIMEYLKQCGKAVPLDITPCDLVKMFLSESK

Target ORF information:

RefSeq Version	XM_007489896.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007489896.2

ORF Insert Sequence:

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGAAGG ATTGTGAAAA CACTGAAGAA 
ATTTGCCATT TGAGGGAGCT GGTGAATAAC TTGCAAGAAC TGATCAAACT GTACAGGAAA 
TACAACTGCA AATTAGCTCT GTCTGAATTT GAGAAGGAGA ACACAACTAC TATTGTATTT 
CGAATGCTTG ACAAAGTTTT GGCCCCAGAA CTTATTCCTT CTATCTTAGA GAAATATGTC 
AAATTGTACA TGAAGGAGTA TAACCTGGAA GAGGAGCCAC TTCTTTTGCT GTACATAAAG 
GATATGTTGG AGAGATATAG TTGCAAGTCT GCATCACTCT TTGAAACTTC CTGGGAATCC 
AAAGCCATGG CAATCATAGG CTGTTTGTCT GATACAGATC TCATCTTCGA TGCTGTGCTG 
CAGATCATGT ATGGAGCTGT GGTGCCCTGG AGCACAGCTG TAGAGCAGCT GGTTAAAAAG 
CACTTGGAAA TGGACCATCC CAAAGTGAAA TTGTTGCAGG AAAGTTACAA GTTAATGGAA 
ATGAAGAAGC TTCTACGAGG GTATGGAATA AGAGATACAA ATCTTTTAAA CAAGGAGATA 
ATGAGAGTGA TCAGATACAT TCTTAAACAA GATGGCCCAA CCTCTCTTGA GGATGCTTTA 
AAAGTAGCCC AAGCATATCT GTTGTCTAAA GATGAAATCT ACAGCTTAAG AGTCATTGAC 
TTGATGAGCA GGGAACAGGG TGAAGAATGC CTTTATCTCT TGAAATCCCT GCCTCCTGCT 
GAAGCTGAGA AAACTGCAGA GAGAGTGATT ATATGGGCAC GCTTAGTGTT ACAGGAAGAG 
CCAGATGACT CCCAAGAGGG GAAGCTAGAG AGGATATCTA TAGCAAGGAC AACTGTAAAT 
ATTATAAAAG CACTACGTGA CATTCAGAAA GAAAATCCTC TGAAGATGGA TGAATGTGAA 
GAAAACTTAA AACTATTTAA AACCATTAGG AGCTTACAGG AAGATTTTGA GCTCTTCCTT 
CCATTTGATG ACTACAGGAA TCATTCACTG GTAGCTGGAC TTCACAAAGA ACCCATTAAC 
TCTCAGGAAG TTTCCCAGCC CTGGCAAAAA ACTAGGAGGA CCCCAGAGGA GACTCCTAGT 
CATTCAAAGA ATGTATCTCT TCAGTCGAGT TTGTACAGGC AGACTCTGTG TCTGTTGGTG 
TCAGAGCAAG ACCTGGAGGC TGAAGTAGTG CTGAAGGCCT TAAATTCAGG GAGAGTTGAC 
GAAGCCCTCC GGCTGTGCAG GGATTTGTTT CAATGTCACT GCAATCCCAA CACGGGAAGA 
GTGCTGGTTT TGGCATGTCA GAAGCTTTGT CAGATGTTGG GGACCGACAC TCCAATGATT 
GTGCCTGACG AGTTGAACCT TCCTCTGGAG ATCTATCAGT TGGCCTGCCA AGCAGCCACT 
GTCTGTAGCC CAGATTTTTT ACTAGATGCT CTGGAAATGT GTAAATATAC TTTAACTGCC 
ATGGAGATTT ACAGACAGTG CCAGATGGAA GATTGTGGAA CTCTAACAAA GACTTCCTTT 
GGAGCTGATA GAGACCCCTA TGAAGAATGG ACATACAATG ACTTCTTCAG TGAAGATGGA 
ATTGTTCTCG AATCCCAACT GGTGCTACCA GTTATCTATG AGCTGATTTC ATCTCTTATA 
CATGAGTCTG GAGGCAAAAG ATACCCTTTG GATTCTGCCA GTTTGCCATA TTGCTCATTT 
CATAAAGGAA AGAATCTTCT TTTACCAATC ATCAACCCTG TCTTAGCTCT GCTTCAGAAC 
CTACAGGAAT CGAGCCAGTG GGAGCTGGCT CTGAAATTTG TAGTAGGTTC CTTTGGCATC 
TGCCTTCAGC ATGGAATGTC CAACTGCATG AATATCTCCT TGAGTGAGAA GCTGTATGAA 
GAGAAACTGT TGATCAGTAC AAAAAACATT ATTGTTGGCA TGAAAGAAAA ATCCACGAAC 
TTGGTCAGAG AAAATGCTGC GGCCCTGTTA CACAAAGTGT TTAATTGTCG TCTAGTGGAT 
CATGACCTGG CCCTGGGTTA CTTAACTCTT TTACCGAAGA AAGATGTCTT TGAAAATATC 
TGGAAAGTCA TCAATACCAC ATGGCAGAAT TACAGCAAGA TTTTGGCTGT ATCCCAGGTT 
GGAGCTCAAC TGGCTTATCT CTATAAAGAA ACAGAAATAA GGCTGAAATT CCAAGAATTA 
ATCATCGATG CCCAGTGGGG CCTTCGCCTT GGGAAACTTG GGATTTCTTT TCAACCAGTG 
TTCAGGGAGC ATTCACTAAG GAAAAAAGAA CTCATGAGCA CACTGGTTCA GAATTCAGAT 
GTGGACACAA CTCTCATTCT GGAATACTGC AGCACATATG AATTGGACAG TGATGCAGCT 
CTTCAACTTT TCATCGAAAC CCTGATTCTT CAGCATAATC ATATTGACCC CTGTGAGGAA 
GACAAGGCCA TGTCTTCTCT GAAACAGCCC CACTCCAAAG TTCTGGCCAA AGCAGTTGAG 
ATTGTGCCCT TACTGAAAAG CACGAGGAAT TTGGTTGTGA GTCTTAGTGG CATTCTGCAC 
AAGTTGGATC CATATGACTA TGAAATTATT GAAGTTGTCT TGAAAATTAT GCAAAAAGCT 
GATGAAAAGG TCACCAATCT CGATATCAAT CAGGGCTTGA GTCTGATGAA GCATTTGAAA 
TCTTATAGAC GAGCATCTCC TCCTGGGAAT CTGGAACATG AGTACATCAT GAACCATACA 
ATGACCATGT CCTCAGCTGC TCAGATTCGG CTGCCTTTTC ACCTGATTTT CTTTGGGAAA 
GTGCAGAACT TCTGGAAGAT ACTTTCTGCT GAGCTCCGGG AGGAGTCCTT CCCAACATTC 
CTGTTGATTG CTAAACTAAT GAAGGTTTCT TTGGATTCTT TGTATGTGTC GACAGTTAAG 
CAAGTCTTTG AAAAAAGCTT GAAGCCAAAG TTGCTGAAGC AGGTGCAGGC TAAAGGCTCA 
ACCCCGAATA ACAAGGAAAT AGCTAAAACC TTCCAGACTT TGCAGTCCTA TTTATTATCT 
ATAGCTAATC CTGAATGGGC TGTTGCTGTT GCCATCAATC TTGCTCAGGA CATTCCAGAA 
GGTTGCTACA AGATAACTAT ATTGAAATTT TGCCTTTATT TGGCCGAGAA ATGGTTAAAG 
AATATTTCAT CCCAGGATGA AACTCATGAG AAAGCTGAGG CCTTACTGAA GAAACTGAGG 
GTGCAGTACC AGAGGTCAGG CACTGAGACT GTGCTCATCA CACATGGCCT GGGCTCTCCA 
GAGCATCTGA GACTGCTAGG GAAGCCAGCA CAACTCATTG CCAGTCTATA TGAGCATGGC 
AGCATCCTGC CGAGGATCCA GGGACCTGCT GACAAAGACT GTCCTGATAT TCATGCAGCA 
GCCAAGGAAA TAGCTGAAAT TAACAACTTG GATTTGGAAA AAATATGGGA TGTGCTTCTT 
GAAAAATGGT TATGTCCAGC GACACAGACC AATGAAATAC CTTCAGAATT CTTTAATCTT 
CAAGGGGATG AAGCCCTCCA AAGAGTTCTA TATCTCCTCC AAATACGTCC CATCGATTAT 
AGTTCAAGGA TGTTATTTTT GGTTGCCACA TCTTCTGTAT CTCCTTTTAC TGTTGGTCAG 
CTGACATTTG CCTATAGAAG ACGAGCTCTT CACTGTCTCT TATGCTTGGC CGACAGAGAA 
ACCATTGAGT CTCTCTTCAA AAAGCCTTAC GAGGAAGTAA AATGTTTTCT GAAATGCATC 
ACTTTCCTGG CCGCATTTGA AATGTTGAAC ATCCCTCTCA CATATGAATC ATTTCACAAC 
AGTCCTAAAG AAGGAATGAT TAAGGGCCTA TGGAAAAATC ATAGCCATGA ACCTATGGCT 
GTGAGATTGG TGACTGAACT TAGTTTGGAA TACAAGGTAT ATGACACTCA GCTTTGGAGT 
GGGCTGTTAC AAAAGCTGCT TGGCTTCAAC ATGATTAGCT ATCTGAGGAA TGTTTTAACA 
GCAATTTCCA ATATACATTC CTTGTGGCAG ATCCCTTACT TTGGGAGAGC CTGGCAGCGG 
GTCTCACAGA TACCACTCAT TTCAGCCTCT TGCCCCCTGA ATCCTACTCA GCTGTTGGCT 
TGTCGTGAGA GTCTGGTGGT TCTTCTAGAG TGTCCTGTTT CCATGGATCT TGATATGATT 
GGAGTTGCCA AGCAGTTTAG CCAACTGGAG CTTCCAGCCT TTGCCTTGGC CTGCCTTCTG 
CTGATGCCCC ACTCAGACAA GAGAGACCAG CAGCTTCAGA ATTTCTTGGG ATGGTGTAAT 
CCTGAGGTTA TTTTACAACA AATAGATGAA CATATGAATA CTGGCCAGCT AGCAGGATTT 
GCCTGTCAAG TGAAGGGCTT TATTCTGAAT CACATCATAA ATAAGAAGCA GTTTGGGATA 
CTGGCAAACA CAAAATACTT TCCATTGTTG AAATTACAAG TGATAAGTAC CCAGAAGGTC 
AAGGAGTTGG TGGACTATCT AGTCAACAAT GACAGTTTGG ATGAAGCCTC ACTCCTTATA 
ATGGAATACT TAAAGCAGTG TGGAAAAGCA GTGCCACTAG ATATCACTCC TTGTGACCTA 
GTGAAGATGT TTCTAAGTGA ATCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_007489897.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007489959.1 Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

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CloneID	OMc18419
Clone ID Related Accession (Same CDS sequence)	XM_007489896.2 , XM_007489897.2
Accession Version	XM_007489897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007489897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGAAGG ATTGTGAAAA CACTGAAGAA 
ATTTGCCATT TGAGGGAGCT GGTGAATAAC TTGCAAGAAC TGATCAAACT GTACAGGAAA 
TACAACTGCA AATTAGCTCT GTCTGAATTT GAGAAGGAGA ACACAACTAC TATTGTATTT 
CGAATGCTTG ACAAAGTTTT GGCCCCAGAA CTTATTCCTT CTATCTTAGA GAAATATGTC 
AAATTGTACA TGAAGGAGTA TAACCTGGAA GAGGAGCCAC TTCTTTTGCT GTACATAAAG 
GATATGTTGG AGAGATATAG TTGCAAGTCT GCATCACTCT TTGAAACTTC CTGGGAATCC 
AAAGCCATGG CAATCATAGG CTGTTTGTCT GATACAGATC TCATCTTCGA TGCTGTGCTG 
CAGATCATGT ATGGAGCTGT GGTGCCCTGG AGCACAGCTG TAGAGCAGCT GGTTAAAAAG 
CACTTGGAAA TGGACCATCC CAAAGTGAAA TTGTTGCAGG AAAGTTACAA GTTAATGGAA 
ATGAAGAAGC TTCTACGAGG GTATGGAATA AGAGATACAA ATCTTTTAAA CAAGGAGATA 
ATGAGAGTGA TCAGATACAT TCTTAAACAA GATGGCCCAA CCTCTCTTGA GGATGCTTTA 
AAAGTAGCCC AAGCATATCT GTTGTCTAAA GATGAAATCT ACAGCTTAAG AGTCATTGAC 
TTGATGAGCA GGGAACAGGG TGAAGAATGC CTTTATCTCT TGAAATCCCT GCCTCCTGCT 
GAAGCTGAGA AAACTGCAGA GAGAGTGATT ATATGGGCAC GCTTAGTGTT ACAGGAAGAG 
CCAGATGACT CCCAAGAGGG GAAGCTAGAG AGGATATCTA TAGCAAGGAC AACTGTAAAT 
ATTATAAAAG CACTACGTGA CATTCAGAAA GAAAATCCTC TGAAGATGGA TGAATGTGAA 
GAAAACTTAA AACTATTTAA AACCATTAGG AGCTTACAGG AAGATTTTGA GCTCTTCCTT 
CCATTTGATG ACTACAGGAA TCATTCACTG GTAGCTGGAC TTCACAAAGA ACCCATTAAC 
TCTCAGGAAG TTTCCCAGCC CTGGCAAAAA ACTAGGAGGA CCCCAGAGGA GACTCCTAGT 
CATTCAAAGA ATGTATCTCT TCAGTCGAGT TTGTACAGGC AGACTCTGTG TCTGTTGGTG 
TCAGAGCAAG ACCTGGAGGC TGAAGTAGTG CTGAAGGCCT TAAATTCAGG GAGAGTTGAC 
GAAGCCCTCC GGCTGTGCAG GGATTTGTTT CAATGTCACT GCAATCCCAA CACGGGAAGA 
GTGCTGGTTT TGGCATGTCA GAAGCTTTGT CAGATGTTGG GGACCGACAC TCCAATGATT 
GTGCCTGACG AGTTGAACCT TCCTCTGGAG ATCTATCAGT TGGCCTGCCA AGCAGCCACT 
GTCTGTAGCC CAGATTTTTT ACTAGATGCT CTGGAAATGT GTAAATATAC TTTAACTGCC 
ATGGAGATTT ACAGACAGTG CCAGATGGAA GATTGTGGAA CTCTAACAAA GACTTCCTTT 
GGAGCTGATA GAGACCCCTA TGAAGAATGG ACATACAATG ACTTCTTCAG TGAAGATGGA 
ATTGTTCTCG AATCCCAACT GGTGCTACCA GTTATCTATG AGCTGATTTC ATCTCTTATA 
CATGAGTCTG GAGGCAAAAG ATACCCTTTG GATTCTGCCA GTTTGCCATA TTGCTCATTT 
CATAAAGGAA AGAATCTTCT TTTACCAATC ATCAACCCTG TCTTAGCTCT GCTTCAGAAC 
CTACAGGAAT CGAGCCAGTG GGAGCTGGCT CTGAAATTTG TAGTAGGTTC CTTTGGCATC 
TGCCTTCAGC ATGGAATGTC CAACTGCATG AATATCTCCT TGAGTGAGAA GCTGTATGAA 
GAGAAACTGT TGATCAGTAC AAAAAACATT ATTGTTGGCA TGAAAGAAAA ATCCACGAAC 
TTGGTCAGAG AAAATGCTGC GGCCCTGTTA CACAAAGTGT TTAATTGTCG TCTAGTGGAT 
CATGACCTGG CCCTGGGTTA CTTAACTCTT TTACCGAAGA AAGATGTCTT TGAAAATATC 
TGGAAAGTCA TCAATACCAC ATGGCAGAAT TACAGCAAGA TTTTGGCTGT ATCCCAGGTT 
GGAGCTCAAC TGGCTTATCT CTATAAAGAA ACAGAAATAA GGCTGAAATT CCAAGAATTA 
ATCATCGATG CCCAGTGGGG CCTTCGCCTT GGGAAACTTG GGATTTCTTT TCAACCAGTG 
TTCAGGGAGC ATTCACTAAG GAAAAAAGAA CTCATGAGCA CACTGGTTCA GAATTCAGAT 
GTGGACACAA CTCTCATTCT GGAATACTGC AGCACATATG AATTGGACAG TGATGCAGCT 
CTTCAACTTT TCATCGAAAC CCTGATTCTT CAGCATAATC ATATTGACCC CTGTGAGGAA 
GACAAGGCCA TGTCTTCTCT GAAACAGCCC CACTCCAAAG TTCTGGCCAA AGCAGTTGAG 
ATTGTGCCCT TACTGAAAAG CACGAGGAAT TTGGTTGTGA GTCTTAGTGG CATTCTGCAC 
AAGTTGGATC CATATGACTA TGAAATTATT GAAGTTGTCT TGAAAATTAT GCAAAAAGCT 
GATGAAAAGG TCACCAATCT CGATATCAAT CAGGGCTTGA GTCTGATGAA GCATTTGAAA 
TCTTATAGAC GAGCATCTCC TCCTGGGAAT CTGGAACATG AGTACATCAT GAACCATACA 
ATGACCATGT CCTCAGCTGC TCAGATTCGG CTGCCTTTTC ACCTGATTTT CTTTGGGAAA 
GTGCAGAACT TCTGGAAGAT ACTTTCTGCT GAGCTCCGGG AGGAGTCCTT CCCAACATTC 
CTGTTGATTG CTAAACTAAT GAAGGTTTCT TTGGATTCTT TGTATGTGTC GACAGTTAAG 
CAAGTCTTTG AAAAAAGCTT GAAGCCAAAG TTGCTGAAGC AGGTGCAGGC TAAAGGCTCA 
ACCCCGAATA ACAAGGAAAT AGCTAAAACC TTCCAGACTT TGCAGTCCTA TTTATTATCT 
ATAGCTAATC CTGAATGGGC TGTTGCTGTT GCCATCAATC TTGCTCAGGA CATTCCAGAA 
GGTTGCTACA AGATAACTAT ATTGAAATTT TGCCTTTATT TGGCCGAGAA ATGGTTAAAG 
AATATTTCAT CCCAGGATGA AACTCATGAG AAAGCTGAGG CCTTACTGAA GAAACTGAGG 
GTGCAGTACC AGAGGTCAGG CACTGAGACT GTGCTCATCA CACATGGCCT GGGCTCTCCA 
GAGCATCTGA GACTGCTAGG GAAGCCAGCA CAACTCATTG CCAGTCTATA TGAGCATGGC 
AGCATCCTGC CGAGGATCCA GGGACCTGCT GACAAAGACT GTCCTGATAT TCATGCAGCA 
GCCAAGGAAA TAGCTGAAAT TAACAACTTG GATTTGGAAA AAATATGGGA TGTGCTTCTT 
GAAAAATGGT TATGTCCAGC GACACAGACC AATGAAATAC CTTCAGAATT CTTTAATCTT 
CAAGGGGATG AAGCCCTCCA AAGAGTTCTA TATCTCCTCC AAATACGTCC CATCGATTAT 
AGTTCAAGGA TGTTATTTTT GGTTGCCACA TCTTCTGTAT CTCCTTTTAC TGTTGGTCAG 
CTGACATTTG CCTATAGAAG ACGAGCTCTT CACTGTCTCT TATGCTTGGC CGACAGAGAA 
ACCATTGAGT CTCTCTTCAA AAAGCCTTAC GAGGAAGTAA AATGTTTTCT GAAATGCATC 
ACTTTCCTGG CCGCATTTGA AATGTTGAAC ATCCCTCTCA CATATGAATC ATTTCACAAC 
AGTCCTAAAG AAGGAATGAT TAAGGGCCTA TGGAAAAATC ATAGCCATGA ACCTATGGCT 
GTGAGATTGG TGACTGAACT TAGTTTGGAA TACAAGGTAT ATGACACTCA GCTTTGGAGT 
GGGCTGTTAC AAAAGCTGCT TGGCTTCAAC ATGATTAGCT ATCTGAGGAA TGTTTTAACA 
GCAATTTCCA ATATACATTC CTTGTGGCAG ATCCCTTACT TTGGGAGAGC CTGGCAGCGG 
GTCTCACAGA TACCACTCAT TTCAGCCTCT TGCCCCCTGA ATCCTACTCA GCTGTTGGCT 
TGTCGTGAGA GTCTGGTGGT TCTTCTAGAG TGTCCTGTTT CCATGGATCT TGATATGATT 
GGAGTTGCCA AGCAGTTTAG CCAACTGGAG CTTCCAGCCT TTGCCTTGGC CTGCCTTCTG 
CTGATGCCCC ACTCAGACAA GAGAGACCAG CAGCTTCAGA ATTTCTTGGG ATGGTGTAAT 
CCTGAGGTTA TTTTACAACA AATAGATGAA CATATGAATA CTGGCCAGCT AGCAGGATTT 
GCCTGTCAAG TGAAGGGCTT TATTCTGAAT CACATCATAA ATAAGAAGCA GTTTGGGATA 
CTGGCAAACA CAAAATACTT TCCATTGTTG AAATTACAAG TGATAAGTAC CCAGAAGGTC 
AAGGAGTTGG TGGACTATCT AGTCAACAAT GACAGTTTGG ATGAAGCCTC ACTCCTTATA 
ATGGAATACT TAAAGCAGTG TGGAAAAGCA GTGCCACTAG ATATCACTCC TTGTGACCTA 
GTGAAGATGT TTCTAAGTGA ATCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007489959.1
CDS	289..6918
Translation	MWDDIELLTSDDTGSGCLSIGSGEEHGTTLYQVDTLVKISSEKASFSPKLHACSSSDGFVIVVDQSVTLFDNICRSLQLHLQFDTKVDVVGLCQGGEFLLVGERSGNLHLIHVTSNQTLLTNAFVQQAPDENQCTYQDLIIEEGGAKDGKYHVFLLTYNGLFCIMDLQLTQIQEAIEKKDFIAAKELQGQTKTSFITTENYHDRGCLSFVIGDLTSENQVIIGGTGDFVLSKWELDPENKVAMVKNFIDSDMIKGAKKIQLADNLLFILDTMNVLSLWDIYTLIPVWNWPCTHFEDFILTTEADSSSSVTWEGVINLKLIALTTSSNQMRNLMVYSLPTMTLLYSLEVSAVSSLVRMGISSDTIYLLEGIYENDHKSPEGSVSMIVLRCLTEALPENRLSRLLHKHKFTEAENFAISFGLDVELVYKVKSNDLLERMALCSVGTHGPTVWQQLVDEAKESLCKIQDDQFVVDYCLKAQWPTFETTQEMLNYAKVRLLKKEDKIPTTFSNGLTEVLKARAKLATFYGAFGPQKFSGNSWIEFLKNEDILKDIYLQLKEGNLFCAQFLWLRHRADFESRFNVNMLDSLLSSISTSIPMRALCLWLQSDVIPFVKRILPEGQGILAKWLEQAARNLELTDKANWPENGLEVAQVFFTSENPDSLGLISSCHCISLKDCENTEEICHLRELVNNLQELIKLYRKYNCKLALSEFEKENTTTIVFRMLDKVLAPELIPSILEKYVKLYMKEYNLEEEPLLLLYIKDMLERYSCKSASLFETSWESKAMAIIGCLSDTDLIFDAVLQIMYGAVVPWSTAVEQLVKKHLEMDHPKVKLLQESYKLMEMKKLLRGYGIRDTNLLNKEIMRVIRYILKQDGPTSLEDALKVAQAYLLSKDEIYSLRVIDLMSREQGEECLYLLKSLPPAEAEKTAERVIIWARLVLQEEPDDSQEGKLERISIARTTVNIIKALRDIQKENPLKMDECEENLKLFKTIRSLQEDFELFLPFDDYRNHSLVAGLHKEPINSQEVSQPWQKTRRTPEETPSHSKNVSLQSSLYRQTLCLLVSEQDLEAEVVLKALNSGRVDEALRLCRDLFQCHCNPNTGRVLVLACQKLCQMLGTDTPMIVPDELNLPLEIYQLACQAATVCSPDFLLDALEMCKYTLTAMEIYRQCQMEDCGTLTKTSFGADRDPYEEWTYNDFFSEDGIVLESQLVLPVIYELISSLIHESGGKRYPLDSASLPYCSFHKGKNLLLPIINPVLALLQNLQESSQWELALKFVVGSFGICLQHGMSNCMNISLSEKLYEEKLLISTKNIIVGMKEKSTNLVRENAAALLHKVFNCRLVDHDLALGYLTLLPKKDVFENIWKVINTTWQNYSKILAVSQVGAQLAYLYKETEIRLKFQELIIDAQWGLRLGKLGISFQPVFREHSLRKKELMSTLVQNSDVDTTLILEYCSTYELDSDAALQLFIETLILQHNHIDPCEEDKAMSSLKQPHSKVLAKAVEIVPLLKSTRNLVVSLSGILHKLDPYDYEIIEVVLKIMQKADEKVTNLDINQGLSLMKHLKSYRRASPPGNLEHEYIMNHTMTMSSAAQIRLPFHLIFFGKVQNFWKILSAELREESFPTFLLIAKLMKVSLDSLYVSTVKQVFEKSLKPKLLKQVQAKGSTPNNKEIAKTFQTLQSYLLSIANPEWAVAVAINLAQDIPEGCYKITILKFCLYLAEKWLKNISSQDETHEKAEALLKKLRVQYQRSGTETVLITHGLGSPEHLRLLGKPAQLIASLYEHGSILPRIQGPADKDCPDIHAAAKEIAEINNLDLEKIWDVLLEKWLCPATQTNEIPSEFFNLQGDEALQRVLYLLQIRPIDYSSRMLFLVATSSVSPFTVGQLTFAYRRRALHCLLCLADRETIESLFKKPYEEVKCFLKCITFLAAFEMLNIPLTYESFHNSPKEGMIKGLWKNHSHEPMAVRLVTELSLEYKVYDTQLWSGLLQKLLGFNMISYLRNVLTAISNIHSLWQIPYFGRAWQRVSQIPLISASCPLNPTQLLACRESLVVLLECPVSMDLDMIGVAKQFSQLELPAFALACLLLMPHSDKRDQQLQNFLGWCNPEVILQQIDEHMNTGQLAGFACQVKGFILNHIINKKQFGILANTKYFPLLKLQVISTQKVKELVDYLVNNDSLDEASLLIMEYLKQCGKAVPLDITPCDLVKMFLSESK

Target ORF information:

RefSeq Version	XM_007489897.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007489897.2

ORF Insert Sequence:

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGAAGG ATTGTGAAAA CACTGAAGAA 
ATTTGCCATT TGAGGGAGCT GGTGAATAAC TTGCAAGAAC TGATCAAACT GTACAGGAAA 
TACAACTGCA AATTAGCTCT GTCTGAATTT GAGAAGGAGA ACACAACTAC TATTGTATTT 
CGAATGCTTG ACAAAGTTTT GGCCCCAGAA CTTATTCCTT CTATCTTAGA GAAATATGTC 
AAATTGTACA TGAAGGAGTA TAACCTGGAA GAGGAGCCAC TTCTTTTGCT GTACATAAAG 
GATATGTTGG AGAGATATAG TTGCAAGTCT GCATCACTCT TTGAAACTTC CTGGGAATCC 
AAAGCCATGG CAATCATAGG CTGTTTGTCT GATACAGATC TCATCTTCGA TGCTGTGCTG 
CAGATCATGT ATGGAGCTGT GGTGCCCTGG AGCACAGCTG TAGAGCAGCT GGTTAAAAAG 
CACTTGGAAA TGGACCATCC CAAAGTGAAA TTGTTGCAGG AAAGTTACAA GTTAATGGAA 
ATGAAGAAGC TTCTACGAGG GTATGGAATA AGAGATACAA ATCTTTTAAA CAAGGAGATA 
ATGAGAGTGA TCAGATACAT TCTTAAACAA GATGGCCCAA CCTCTCTTGA GGATGCTTTA 
AAAGTAGCCC AAGCATATCT GTTGTCTAAA GATGAAATCT ACAGCTTAAG AGTCATTGAC 
TTGATGAGCA GGGAACAGGG TGAAGAATGC CTTTATCTCT TGAAATCCCT GCCTCCTGCT 
GAAGCTGAGA AAACTGCAGA GAGAGTGATT ATATGGGCAC GCTTAGTGTT ACAGGAAGAG 
CCAGATGACT CCCAAGAGGG GAAGCTAGAG AGGATATCTA TAGCAAGGAC AACTGTAAAT 
ATTATAAAAG CACTACGTGA CATTCAGAAA GAAAATCCTC TGAAGATGGA TGAATGTGAA 
GAAAACTTAA AACTATTTAA AACCATTAGG AGCTTACAGG AAGATTTTGA GCTCTTCCTT 
CCATTTGATG ACTACAGGAA TCATTCACTG GTAGCTGGAC TTCACAAAGA ACCCATTAAC 
TCTCAGGAAG TTTCCCAGCC CTGGCAAAAA ACTAGGAGGA CCCCAGAGGA GACTCCTAGT 
CATTCAAAGA ATGTATCTCT TCAGTCGAGT TTGTACAGGC AGACTCTGTG TCTGTTGGTG 
TCAGAGCAAG ACCTGGAGGC TGAAGTAGTG CTGAAGGCCT TAAATTCAGG GAGAGTTGAC 
GAAGCCCTCC GGCTGTGCAG GGATTTGTTT CAATGTCACT GCAATCCCAA CACGGGAAGA 
GTGCTGGTTT TGGCATGTCA GAAGCTTTGT CAGATGTTGG GGACCGACAC TCCAATGATT 
GTGCCTGACG AGTTGAACCT TCCTCTGGAG ATCTATCAGT TGGCCTGCCA AGCAGCCACT 
GTCTGTAGCC CAGATTTTTT ACTAGATGCT CTGGAAATGT GTAAATATAC TTTAACTGCC 
ATGGAGATTT ACAGACAGTG CCAGATGGAA GATTGTGGAA CTCTAACAAA GACTTCCTTT 
GGAGCTGATA GAGACCCCTA TGAAGAATGG ACATACAATG ACTTCTTCAG TGAAGATGGA 
ATTGTTCTCG AATCCCAACT GGTGCTACCA GTTATCTATG AGCTGATTTC ATCTCTTATA 
CATGAGTCTG GAGGCAAAAG ATACCCTTTG GATTCTGCCA GTTTGCCATA TTGCTCATTT 
CATAAAGGAA AGAATCTTCT TTTACCAATC ATCAACCCTG TCTTAGCTCT GCTTCAGAAC 
CTACAGGAAT CGAGCCAGTG GGAGCTGGCT CTGAAATTTG TAGTAGGTTC CTTTGGCATC 
TGCCTTCAGC ATGGAATGTC CAACTGCATG AATATCTCCT TGAGTGAGAA GCTGTATGAA 
GAGAAACTGT TGATCAGTAC AAAAAACATT ATTGTTGGCA TGAAAGAAAA ATCCACGAAC 
TTGGTCAGAG AAAATGCTGC GGCCCTGTTA CACAAAGTGT TTAATTGTCG TCTAGTGGAT 
CATGACCTGG CCCTGGGTTA CTTAACTCTT TTACCGAAGA AAGATGTCTT TGAAAATATC 
TGGAAAGTCA TCAATACCAC ATGGCAGAAT TACAGCAAGA TTTTGGCTGT ATCCCAGGTT 
GGAGCTCAAC TGGCTTATCT CTATAAAGAA ACAGAAATAA GGCTGAAATT CCAAGAATTA 
ATCATCGATG CCCAGTGGGG CCTTCGCCTT GGGAAACTTG GGATTTCTTT TCAACCAGTG 
TTCAGGGAGC ATTCACTAAG GAAAAAAGAA CTCATGAGCA CACTGGTTCA GAATTCAGAT 
GTGGACACAA CTCTCATTCT GGAATACTGC AGCACATATG AATTGGACAG TGATGCAGCT 
CTTCAACTTT TCATCGAAAC CCTGATTCTT CAGCATAATC ATATTGACCC CTGTGAGGAA 
GACAAGGCCA TGTCTTCTCT GAAACAGCCC CACTCCAAAG TTCTGGCCAA AGCAGTTGAG 
ATTGTGCCCT TACTGAAAAG CACGAGGAAT TTGGTTGTGA GTCTTAGTGG CATTCTGCAC 
AAGTTGGATC CATATGACTA TGAAATTATT GAAGTTGTCT TGAAAATTAT GCAAAAAGCT 
GATGAAAAGG TCACCAATCT CGATATCAAT CAGGGCTTGA GTCTGATGAA GCATTTGAAA 
TCTTATAGAC GAGCATCTCC TCCTGGGAAT CTGGAACATG AGTACATCAT GAACCATACA 
ATGACCATGT CCTCAGCTGC TCAGATTCGG CTGCCTTTTC ACCTGATTTT CTTTGGGAAA 
GTGCAGAACT TCTGGAAGAT ACTTTCTGCT GAGCTCCGGG AGGAGTCCTT CCCAACATTC 
CTGTTGATTG CTAAACTAAT GAAGGTTTCT TTGGATTCTT TGTATGTGTC GACAGTTAAG 
CAAGTCTTTG AAAAAAGCTT GAAGCCAAAG TTGCTGAAGC AGGTGCAGGC TAAAGGCTCA 
ACCCCGAATA ACAAGGAAAT AGCTAAAACC TTCCAGACTT TGCAGTCCTA TTTATTATCT 
ATAGCTAATC CTGAATGGGC TGTTGCTGTT GCCATCAATC TTGCTCAGGA CATTCCAGAA 
GGTTGCTACA AGATAACTAT ATTGAAATTT TGCCTTTATT TGGCCGAGAA ATGGTTAAAG 
AATATTTCAT CCCAGGATGA AACTCATGAG AAAGCTGAGG CCTTACTGAA GAAACTGAGG 
GTGCAGTACC AGAGGTCAGG CACTGAGACT GTGCTCATCA CACATGGCCT GGGCTCTCCA 
GAGCATCTGA GACTGCTAGG GAAGCCAGCA CAACTCATTG CCAGTCTATA TGAGCATGGC 
AGCATCCTGC CGAGGATCCA GGGACCTGCT GACAAAGACT GTCCTGATAT TCATGCAGCA 
GCCAAGGAAA TAGCTGAAAT TAACAACTTG GATTTGGAAA AAATATGGGA TGTGCTTCTT 
GAAAAATGGT TATGTCCAGC GACACAGACC AATGAAATAC CTTCAGAATT CTTTAATCTT 
CAAGGGGATG AAGCCCTCCA AAGAGTTCTA TATCTCCTCC AAATACGTCC CATCGATTAT 
AGTTCAAGGA TGTTATTTTT GGTTGCCACA TCTTCTGTAT CTCCTTTTAC TGTTGGTCAG 
CTGACATTTG CCTATAGAAG ACGAGCTCTT CACTGTCTCT TATGCTTGGC CGACAGAGAA 
ACCATTGAGT CTCTCTTCAA AAAGCCTTAC GAGGAAGTAA AATGTTTTCT GAAATGCATC 
ACTTTCCTGG CCGCATTTGA AATGTTGAAC ATCCCTCTCA CATATGAATC ATTTCACAAC 
AGTCCTAAAG AAGGAATGAT TAAGGGCCTA TGGAAAAATC ATAGCCATGA ACCTATGGCT 
GTGAGATTGG TGACTGAACT TAGTTTGGAA TACAAGGTAT ATGACACTCA GCTTTGGAGT 
GGGCTGTTAC AAAAGCTGCT TGGCTTCAAC ATGATTAGCT ATCTGAGGAA TGTTTTAACA 
GCAATTTCCA ATATACATTC CTTGTGGCAG ATCCCTTACT TTGGGAGAGC CTGGCAGCGG 
GTCTCACAGA TACCACTCAT TTCAGCCTCT TGCCCCCTGA ATCCTACTCA GCTGTTGGCT 
TGTCGTGAGA GTCTGGTGGT TCTTCTAGAG TGTCCTGTTT CCATGGATCT TGATATGATT 
GGAGTTGCCA AGCAGTTTAG CCAACTGGAG CTTCCAGCCT TTGCCTTGGC CTGCCTTCTG 
CTGATGCCCC ACTCAGACAA GAGAGACCAG CAGCTTCAGA ATTTCTTGGG ATGGTGTAAT 
CCTGAGGTTA TTTTACAACA AATAGATGAA CATATGAATA CTGGCCAGCT AGCAGGATTT 
GCCTGTCAAG TGAAGGGCTT TATTCTGAAT CACATCATAA ATAAGAAGCA GTTTGGGATA 
CTGGCAAACA CAAAATACTT TCCATTGTTG AAATTACAAG TGATAAGTAC CCAGAAGGTC 
AAGGAGTTGG TGGACTATCT AGTCAACAAT GACAGTTTGG ATGAAGCCTC ACTCCTTATA 
ATGGAATACT TAAAGCAGTG TGGAAAAGCA GTGCCACTAG ATATCACTCC TTGTGACCTA 
GTGAAGATGT TTCTAAGTGA ATCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_007489898.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007489960.1 Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

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CloneID	OMc18420
Clone ID Related Accession (Same CDS sequence)	XM_007489898.2
Accession Version	XM_007489898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007489898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGGATT GTGAAAACAC TGAAGAAATT 
TGCCATTTGA GGGAGCTGGT GAATAACTTG CAAGAACTGA TCAAACTGTA CAGGAAATAC 
AACTGCAAAT TAGCTCTGTC TGAATTTGAG AAGGAGAACA CAACTACTAT TGTATTTCGA 
ATGCTTGACA AAGTTTTGGC CCCAGAACTT ATTCCTTCTA TCTTAGAGAA ATATGTCAAA 
TTGTACATGA AGGAGTATAA CCTGGAAGAG GAGCCACTTC TTTTGCTGTA CATAAAGGAT 
ATGTTGGAGA GATATAGTTG CAAGTCTGCA TCACTCTTTG AAACTTCCTG GGAATCCAAA 
GCCATGGCAA TCATAGGCTG TTTGTCTGAT ACAGATCTCA TCTTCGATGC TGTGCTGCAG 
ATCATGTATG GAGCTGTGGT GCCCTGGAGC ACAGCTGTAG AGCAGCTGGT TAAAAAGCAC 
TTGGAAATGG ACCATCCCAA AGTGAAATTG TTGCAGGAAA GTTACAAGTT AATGGAAATG 
AAGAAGCTTC TACGAGGGTA TGGAATAAGA GATACAAATC TTTTAAACAA GGAGATAATG 
AGAGTGATCA GATACATTCT TAAACAAGAT GGCCCAACCT CTCTTGAGGA TGCTTTAAAA 
GTAGCCCAAG CATATCTGTT GTCTAAAGAT GAAATCTACA GCTTAAGAGT CATTGACTTG 
ATGAGCAGGG AACAGGGTGA AGAATGCCTT TATCTCTTGA AATCCCTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAGAG AGTGATTATA TGGGCACGCT TAGTGTTACA GGAAGAGCCA 
GATGACTCCC AAGAGGGGAA GCTAGAGAGG ATATCTATAG CAAGGACAAC TGTAAATATT 
ATAAAAGCAC TACGTGACAT TCAGAAAGAA AATCCTCTGA AGATGGATGA ATGTGAAGAA 
AACTTAAAAC TATTTAAAAC CATTAGGAGC TTACAGGAAG ATTTTGAGCT CTTCCTTCCA 
TTTGATGACT ACAGGAATCA TTCACTGGTA GCTGGACTTC ACAAAGAACC CATTAACTCT 
CAGGAAGTTT CCCAGCCCTG GCAAAAAACT AGGAGGACCC CAGAGGAGAC TCCTAGTCAT 
TCAAAGAATG TATCTCTTCA GTCGAGTTTG TACAGGCAGA CTCTGTGTCT GTTGGTGTCA 
GAGCAAGACC TGGAGGCTGA AGTAGTGCTG AAGGCCTTAA ATTCAGGGAG AGTTGACGAA 
GCCCTCCGGC TGTGCAGGGA TTTGTTTCAA TGTCACTGCA ATCCCAACAC GGGAAGAGTG 
CTGGTTTTGG CATGTCAGAA GCTTTGTCAG ATGTTGGGGA CCGACACTCC AATGATTGTG 
CCTGACGAGT TGAACCTTCC TCTGGAGATC TATCAGTTGG CCTGCCAAGC AGCCACTGTC 
TGTAGCCCAG ATTTTTTACT AGATGCTCTG GAAATGTGTA AATATACTTT AACTGCCATG 
GAGATTTACA GACAGTGCCA GATGGAAGAT TGTGGAACTC TAACAAAGAC TTCCTTTGGA 
GCTGATAGAG ACCCCTATGA AGAATGGACA TACAATGACT TCTTCAGTGA AGATGGAATT 
GTTCTCGAAT CCCAACTGGT GCTACCAGTT ATCTATGAGC TGATTTCATC TCTTATACAT 
GAGTCTGGAG GCAAAAGATA CCCTTTGGAT TCTGCCAGTT TGCCATATTG CTCATTTCAT 
AAAGGAAAGA ATCTTCTTTT ACCAATCATC AACCCTGTCT TAGCTCTGCT TCAGAACCTA 
CAGGAATCGA GCCAGTGGGA GCTGGCTCTG AAATTTGTAG TAGGTTCCTT TGGCATCTGC 
CTTCAGCATG GAATGTCCAA CTGCATGAAT ATCTCCTTGA GTGAGAAGCT GTATGAAGAG 
AAACTGTTGA TCAGTACAAA AAACATTATT GTTGGCATGA AAGAAAAATC CACGAACTTG 
GTCAGAGAAA ATGCTGCGGC CCTGTTACAC AAAGTGTTTA ATTGTCGTCT AGTGGATCAT 
GACCTGGCCC TGGGTTACTT AACTCTTTTA CCGAAGAAAG ATGTCTTTGA AAATATCTGG 
AAAGTCATCA ATACCACATG GCAGAATTAC AGCAAGATTT TGGCTGTATC CCAGGTTGGA 
GCTCAACTGG CTTATCTCTA TAAAGAAACA GAAATAAGGC TGAAATTCCA AGAATTAATC 
ATCGATGCCC AGTGGGGCCT TCGCCTTGGG AAACTTGGGA TTTCTTTTCA ACCAGTGTTC 
AGGGAGCATT CACTAAGGAA AAAAGAACTC ATGAGCACAC TGGTTCAGAA TTCAGATGTG 
GACACAACTC TCATTCTGGA ATACTGCAGC ACATATGAAT TGGACAGTGA TGCAGCTCTT 
CAACTTTTCA TCGAAACCCT GATTCTTCAG CATAATCATA TTGACCCCTG TGAGGAAGAC 
AAGGCCATGT CTTCTCTGAA ACAGCCCCAC TCCAAAGTTC TGGCCAAAGC AGTTGAGATT 
GTGCCCTTAC TGAAAAGCAC GAGGAATTTG GTTGTGAGTC TTAGTGGCAT TCTGCACAAG 
TTGGATCCAT ATGACTATGA AATTATTGAA GTTGTCTTGA AAATTATGCA AAAAGCTGAT 
GAAAAGGTCA CCAATCTCGA TATCAATCAG GGCTTGAGTC TGATGAAGCA TTTGAAATCT 
TATAGACGAG CATCTCCTCC TGGGAATCTG GAACATGAGT ACATCATGAA CCATACAATG 
ACCATGTCCT CAGCTGCTCA GATTCGGCTG CCTTTTCACC TGATTTTCTT TGGGAAAGTG 
CAGAACTTCT GGAAGATACT TTCTGCTGAG CTCCGGGAGG AGTCCTTCCC AACATTCCTG 
TTGATTGCTA AACTAATGAA GGTTTCTTTG GATTCTTTGT ATGTGTCGAC AGTTAAGCAA 
GTCTTTGAAA AAAGCTTGAA GCCAAAGTTG CTGAAGCAGG TGCAGGCTAA AGGCTCAACC 
CCGAATAACA AGGAAATAGC TAAAACCTTC CAGACTTTGC AGTCCTATTT ATTATCTATA 
GCTAATCCTG AATGGGCTGT TGCTGTTGCC ATCAATCTTG CTCAGGACAT TCCAGAAGGT 
TGCTACAAGA TAACTATATT GAAATTTTGC CTTTATTTGG CCGAGAAATG GTTAAAGAAT 
ATTTCATCCC AGGATGAAAC TCATGAGAAA GCTGAGGCCT TACTGAAGAA ACTGAGGGTG 
CAGTACCAGA GGTCAGGCAC TGAGACTGTG CTCATCACAC ATGGCCTGGG CTCTCCAGAG 
CATCTGAGAC TGCTAGGGAA GCCAGCACAA CTCATTGCCA GTCTATATGA GCATGGCAGC 
ATCCTGCCGA GGATCCAGGG ACCTGCTGAC AAAGACTGTC CTGATATTCA TGCAGCAGCC 
AAGGAAATAG CTGAAATTAA CAACTTGGAT TTGGAAAAAA TATGGGATGT GCTTCTTGAA 
AAATGGTTAT GTCCAGCGAC ACAGACCAAT GAAATACCTT CAGAATTCTT TAATCTTCAA 
GGGGATGAAG CCCTCCAAAG AGTTCTATAT CTCCTCCAAA TACGTCCCAT CGATTATAGT 
TCAAGGATGT TATTTTTGGT TGCCACATCT TCTGTATCTC CTTTTACTGT TGGTCAGCTG 
ACATTTGCCT ATAGAAGACG AGCTCTTCAC TGTCTCTTAT GCTTGGCCGA CAGAGAAACC 
ATTGAGTCTC TCTTCAAAAA GCCTTACGAG GAAGTAAAAT GTTTTCTGAA ATGCATCACT 
TTCCTGGCCG CATTTGAAAT GTTGAACATC CCTCTCACAT ATGAATCATT TCACAACAGT 
CCTAAAGAAG GAATGATTAA GGGCCTATGG AAAAATCATA GCCATGAACC TATGGCTGTG 
AGATTGGTGA CTGAACTTAG TTTGGAATAC AAGGTATATG ACACTCAGCT TTGGAGTGGG 
CTGTTACAAA AGCTGCTTGG CTTCAACATG ATTAGCTATC TGAGGAATGT TTTAACAGCA 
ATTTCCAATA TACATTCCTT GTGGCAGATC CCTTACTTTG GGAGAGCCTG GCAGCGGGTC 
TCACAGATAC CACTCATTTC AGCCTCTTGC CCCCTGAATC CTACTCAGCT GTTGGCTTGT 
CGTGAGAGTC TGGTGGTTCT TCTAGAGTGT CCTGTTTCCA TGGATCTTGA TATGATTGGA 
GTTGCCAAGC AGTTTAGCCA ACTGGAGCTT CCAGCCTTTG CCTTGGCCTG CCTTCTGCTG 
ATGCCCCACT CAGACAAGAG AGACCAGCAG CTTCAGAATT TCTTGGGATG GTGTAATCCT 
GAGGTTATTT TACAACAAAT AGATGAACAT ATGAATACTG GCCAGCTAGC AGGATTTGCC 
TGTCAAGTGA AGGGCTTTAT TCTGAATCAC ATCATAAATA AGAAGCAGTT TGGGATACTG 
GCAAACACAA AATACTTTCC ATTGTTGAAA TTACAAGTGA TAAGTACCCA GAAGGTCAAG 
GAGTTGGTGG ACTATCTAGT CAACAATGAC AGTTTGGATG AAGCCTCACT CCTTATAATG 
GAATACTTAA AGCAGTGTGG AAAAGCAGTG CCACTAGATA TCACTCCTTG TGACCTAGTG 
AAGATGTTTC TAAGTGAATC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007489960.1
CDS	402..7028
Translation	MWDDIELLTSDDTGSGCLSIGSGEEHGTTLYQVDTLVKISSEKASFSPKLHACSSSDGFVIVVDQSVTLFDNICRSLQLHLQFDTKVDVVGLCQGGEFLLVGERSGNLHLIHVTSNQTLLTNAFVQQAPDENQCTYQDLIIEEGGAKDGKYHVFLLTYNGLFCIMDLQLTQIQEAIEKKDFIAAKELQGQTKTSFITTENYHDRGCLSFVIGDLTSENQVIIGGTGDFVLSKWELDPENKVAMVKNFIDSDMIKGAKKIQLADNLLFILDTMNVLSLWDIYTLIPVWNWPCTHFEDFILTTEADSSSSVTWEGVINLKLIALTTSSNQMRNLMVYSLPTMTLLYSLEVSAVSSLVRMGISSDTIYLLEGIYENDHKSPEGSVSMIVLRCLTEALPENRLSRLLHKHKFTEAENFAISFGLDVELVYKVKSNDLLERMALCSVGTHGPTVWQQLVDEAKESLCKIQDDQFVVDYCLKAQWPTFETTQEMLNYAKVRLLKKEDKIPTTFSNGLTEVLKARAKLATFYGAFGPQKFSGNSWIEFLKNEDILKDIYLQLKEGNLFCAQFLWLRHRADFESRFNVNMLDSLLSSISTSIPMRALCLWLQSDVIPFVKRILPEGQGILAKWLEQAARNLELTDKANWPENGLEVAQVFFTSENPDSLGLISSCHCISLDCENTEEICHLRELVNNLQELIKLYRKYNCKLALSEFEKENTTTIVFRMLDKVLAPELIPSILEKYVKLYMKEYNLEEEPLLLLYIKDMLERYSCKSASLFETSWESKAMAIIGCLSDTDLIFDAVLQIMYGAVVPWSTAVEQLVKKHLEMDHPKVKLLQESYKLMEMKKLLRGYGIRDTNLLNKEIMRVIRYILKQDGPTSLEDALKVAQAYLLSKDEIYSLRVIDLMSREQGEECLYLLKSLPPAEAEKTAERVIIWARLVLQEEPDDSQEGKLERISIARTTVNIIKALRDIQKENPLKMDECEENLKLFKTIRSLQEDFELFLPFDDYRNHSLVAGLHKEPINSQEVSQPWQKTRRTPEETPSHSKNVSLQSSLYRQTLCLLVSEQDLEAEVVLKALNSGRVDEALRLCRDLFQCHCNPNTGRVLVLACQKLCQMLGTDTPMIVPDELNLPLEIYQLACQAATVCSPDFLLDALEMCKYTLTAMEIYRQCQMEDCGTLTKTSFGADRDPYEEWTYNDFFSEDGIVLESQLVLPVIYELISSLIHESGGKRYPLDSASLPYCSFHKGKNLLLPIINPVLALLQNLQESSQWELALKFVVGSFGICLQHGMSNCMNISLSEKLYEEKLLISTKNIIVGMKEKSTNLVRENAAALLHKVFNCRLVDHDLALGYLTLLPKKDVFENIWKVINTTWQNYSKILAVSQVGAQLAYLYKETEIRLKFQELIIDAQWGLRLGKLGISFQPVFREHSLRKKELMSTLVQNSDVDTTLILEYCSTYELDSDAALQLFIETLILQHNHIDPCEEDKAMSSLKQPHSKVLAKAVEIVPLLKSTRNLVVSLSGILHKLDPYDYEIIEVVLKIMQKADEKVTNLDINQGLSLMKHLKSYRRASPPGNLEHEYIMNHTMTMSSAAQIRLPFHLIFFGKVQNFWKILSAELREESFPTFLLIAKLMKVSLDSLYVSTVKQVFEKSLKPKLLKQVQAKGSTPNNKEIAKTFQTLQSYLLSIANPEWAVAVAINLAQDIPEGCYKITILKFCLYLAEKWLKNISSQDETHEKAEALLKKLRVQYQRSGTETVLITHGLGSPEHLRLLGKPAQLIASLYEHGSILPRIQGPADKDCPDIHAAAKEIAEINNLDLEKIWDVLLEKWLCPATQTNEIPSEFFNLQGDEALQRVLYLLQIRPIDYSSRMLFLVATSSVSPFTVGQLTFAYRRRALHCLLCLADRETIESLFKKPYEEVKCFLKCITFLAAFEMLNIPLTYESFHNSPKEGMIKGLWKNHSHEPMAVRLVTELSLEYKVYDTQLWSGLLQKLLGFNMISYLRNVLTAISNIHSLWQIPYFGRAWQRVSQIPLISASCPLNPTQLLACRESLVVLLECPVSMDLDMIGVAKQFSQLELPAFALACLLLMPHSDKRDQQLQNFLGWCNPEVILQQIDEHMNTGQLAGFACQVKGFILNHIINKKQFGILANTKYFPLLKLQVISTQKVKELVDYLVNNDSLDEASLLIMEYLKQCGKAVPLDITPCDLVKMFLSESK

Target ORF information:

RefSeq Version	XM_007489898.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007489898.2

ORF Insert Sequence:

ATGTGGGATG ATATTGAACT TCTAACTAGT GATGACACAG GAAGTGGTTG CCTAAGTATT 
GGCTCAGGAG AAGAACATGG AACAACTTTA TATCAAGTGG ATACATTAGT CAAGATATCT 
TCTGAGAAGG CTTCCTTTTC TCCCAAGTTG CATGCATGCA GCTCAAGTGA TGGGTTTGTT 
ATCGTGGTTG ATCAGTCAGT GACACTTTTT GACAATATTT GTCGGTCTCT CCAACTGCAT 
CTTCAATTTG ATACTAAAGT GGATGTGGTT GGCTTGTGTC AAGGAGGAGA GTTTCTTTTG 
GTCGGTGAAA GAAGTGGCAA CTTACATCTT ATTCATGTGA CATCAAACCA GACGTTGCTT 
ACTAATGCAT TTGTTCAGCA AGCACCAGAT GAAAATCAAT GTACTTATCA GGATCTGATC 
ATAGAAGAAG GTGGCGCAAA GGATGGTAAA TATCATGTGT TCCTTCTTAC TTACAATGGA 
TTGTTTTGCA TTATGGATCT ACAGCTTACA CAGATTCAGG AAGCCATTGA GAAGAAGGAC 
TTCATAGCAG CAAAGGAGTT ACAAGGACAA ACCAAGACCA GTTTTATAAC TACTGAAAAT 
TATCATGACC GTGGTTGTTT AAGCTTTGTG ATTGGAGATT TAACAAGTGA AAACCAAGTG 
ATAATTGGGG GAACTGGTGA TTTTGTCCTC TCTAAGTGGG AGCTAGATCC TGAGAACAAA 
GTGGCGATGG TTAAGAATTT TATTGATTCA GACATGATTA AAGGTGCTAA GAAGATTCAA 
CTTGCAGACA ATCTGCTTTT CATTTTGGAT ACTATGAATG TATTGAGCTT ATGGGACATT 
TATACTCTAA TTCCTGTATG GAACTGGCCT TGTACCCATT TTGAAGATTT TATTCTTACC 
ACAGAGGCAG ATTCTTCTTC ATCAGTCACT TGGGAAGGTG TTATCAATCT GAAATTAATA 
GCTCTGACTA CTTCTAGTAA TCAGATGAGA AACCTCATGG TTTATTCACT GCCTACAATG 
ACCCTGCTCT ATTCTTTGGA AGTTTCTGCT GTTTCTTCAC TTGTCAGGAT GGGAATCAGC 
TCAGATACAA TTTACCTTCT GGAAGGGATT TATGAAAATG ACCACAAGTC CCCTGAAGGC 
TCAGTGTCCA TGATAGTACT TAGGTGTCTA ACAGAAGCCT TACCAGAAAA CAGATTGAGT 
AGATTGCTTC ACAAGCACAA GTTTACAGAA GCTGAAAACT TTGCCATCAG TTTTGGACTG 
GATGTTGAGC TTGTTTATAA GGTAAAATCA AATGACCTAT TGGAGAGAAT GGCTTTGTGT 
TCAGTAGGCA CTCATGGGCC AACAGTTTGG CAACAGCTTG TGGATGAAGC TAAAGAAAGT 
TTATGTAAAA TCCAGGATGA CCAGTTTGTG GTGGATTACT GCTTAAAGGC TCAGTGGCCA 
ACCTTCGAGA CAACTCAAGA AATGCTGAAT TATGCCAAAG TCAGACTTTT GAAGAAAGAA 
GATAAAATAC CTACTACCTT TTCAAATGGC TTAACAGAGG TATTAAAAGC CCGTGCAAAG 
TTGGCTACTT TTTATGGAGC ATTTGGACCA CAAAAATTCA GTGGAAACAG TTGGATTGAA 
TTTTTGAAAA ATGAAGATAT TCTTAAGGAT ATCTATCTTC AGCTAAAAGA GGGAAACCTT 
TTTTGTGCTC AGTTTCTTTG GCTTCGCCAT CGGGCAGATT TTGAGAGCAG ATTTAATGTG 
AACATGCTGG ACAGTTTGCT GAGCTCAATT AGCACAAGTA TCCCCATGCG AGCCCTTTGT 
CTTTGGCTTC AGAGTGATGT GATTCCATTT GTTAAAAGAA TCTTACCAGA AGGACAGGGA 
ATCCTTGCAA AGTGGTTAGA GCAAGCAGCA AGGAATCTTG AATTAACTGA TAAGGCAAAC 
TGGCCGGAGA ATGGACTTGA AGTGGCACAA GTGTTTTTTA CATCTGAAAA TCCAGATTCT 
TTAGGACTGA TCTCTTCCTG TCACTGCATC TCACTGGATT GTGAAAACAC TGAAGAAATT 
TGCCATTTGA GGGAGCTGGT GAATAACTTG CAAGAACTGA TCAAACTGTA CAGGAAATAC 
AACTGCAAAT TAGCTCTGTC TGAATTTGAG AAGGAGAACA CAACTACTAT TGTATTTCGA 
ATGCTTGACA AAGTTTTGGC CCCAGAACTT ATTCCTTCTA TCTTAGAGAA ATATGTCAAA 
TTGTACATGA AGGAGTATAA CCTGGAAGAG GAGCCACTTC TTTTGCTGTA CATAAAGGAT 
ATGTTGGAGA GATATAGTTG CAAGTCTGCA TCACTCTTTG AAACTTCCTG GGAATCCAAA 
GCCATGGCAA TCATAGGCTG TTTGTCTGAT ACAGATCTCA TCTTCGATGC TGTGCTGCAG 
ATCATGTATG GAGCTGTGGT GCCCTGGAGC ACAGCTGTAG AGCAGCTGGT TAAAAAGCAC 
TTGGAAATGG ACCATCCCAA AGTGAAATTG TTGCAGGAAA GTTACAAGTT AATGGAAATG 
AAGAAGCTTC TACGAGGGTA TGGAATAAGA GATACAAATC TTTTAAACAA GGAGATAATG 
AGAGTGATCA GATACATTCT TAAACAAGAT GGCCCAACCT CTCTTGAGGA TGCTTTAAAA 
GTAGCCCAAG CATATCTGTT GTCTAAAGAT GAAATCTACA GCTTAAGAGT CATTGACTTG 
ATGAGCAGGG AACAGGGTGA AGAATGCCTT TATCTCTTGA AATCCCTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAGAG AGTGATTATA TGGGCACGCT TAGTGTTACA GGAAGAGCCA 
GATGACTCCC AAGAGGGGAA GCTAGAGAGG ATATCTATAG CAAGGACAAC TGTAAATATT 
ATAAAAGCAC TACGTGACAT TCAGAAAGAA AATCCTCTGA AGATGGATGA ATGTGAAGAA 
AACTTAAAAC TATTTAAAAC CATTAGGAGC TTACAGGAAG ATTTTGAGCT CTTCCTTCCA 
TTTGATGACT ACAGGAATCA TTCACTGGTA GCTGGACTTC ACAAAGAACC CATTAACTCT 
CAGGAAGTTT CCCAGCCCTG GCAAAAAACT AGGAGGACCC CAGAGGAGAC TCCTAGTCAT 
TCAAAGAATG TATCTCTTCA GTCGAGTTTG TACAGGCAGA CTCTGTGTCT GTTGGTGTCA 
GAGCAAGACC TGGAGGCTGA AGTAGTGCTG AAGGCCTTAA ATTCAGGGAG AGTTGACGAA 
GCCCTCCGGC TGTGCAGGGA TTTGTTTCAA TGTCACTGCA ATCCCAACAC GGGAAGAGTG 
CTGGTTTTGG CATGTCAGAA GCTTTGTCAG ATGTTGGGGA CCGACACTCC AATGATTGTG 
CCTGACGAGT TGAACCTTCC TCTGGAGATC TATCAGTTGG CCTGCCAAGC AGCCACTGTC 
TGTAGCCCAG ATTTTTTACT AGATGCTCTG GAAATGTGTA AATATACTTT AACTGCCATG 
GAGATTTACA GACAGTGCCA GATGGAAGAT TGTGGAACTC TAACAAAGAC TTCCTTTGGA 
GCTGATAGAG ACCCCTATGA AGAATGGACA TACAATGACT TCTTCAGTGA AGATGGAATT 
GTTCTCGAAT CCCAACTGGT GCTACCAGTT ATCTATGAGC TGATTTCATC TCTTATACAT 
GAGTCTGGAG GCAAAAGATA CCCTTTGGAT TCTGCCAGTT TGCCATATTG CTCATTTCAT 
AAAGGAAAGA ATCTTCTTTT ACCAATCATC AACCCTGTCT TAGCTCTGCT TCAGAACCTA 
CAGGAATCGA GCCAGTGGGA GCTGGCTCTG AAATTTGTAG TAGGTTCCTT TGGCATCTGC 
CTTCAGCATG GAATGTCCAA CTGCATGAAT ATCTCCTTGA GTGAGAAGCT GTATGAAGAG 
AAACTGTTGA TCAGTACAAA AAACATTATT GTTGGCATGA AAGAAAAATC CACGAACTTG 
GTCAGAGAAA ATGCTGCGGC CCTGTTACAC AAAGTGTTTA ATTGTCGTCT AGTGGATCAT 
GACCTGGCCC TGGGTTACTT AACTCTTTTA CCGAAGAAAG ATGTCTTTGA AAATATCTGG 
AAAGTCATCA ATACCACATG GCAGAATTAC AGCAAGATTT TGGCTGTATC CCAGGTTGGA 
GCTCAACTGG CTTATCTCTA TAAAGAAACA GAAATAAGGC TGAAATTCCA AGAATTAATC 
ATCGATGCCC AGTGGGGCCT TCGCCTTGGG AAACTTGGGA TTTCTTTTCA ACCAGTGTTC 
AGGGAGCATT CACTAAGGAA AAAAGAACTC ATGAGCACAC TGGTTCAGAA TTCAGATGTG 
GACACAACTC TCATTCTGGA ATACTGCAGC ACATATGAAT TGGACAGTGA TGCAGCTCTT 
CAACTTTTCA TCGAAACCCT GATTCTTCAG CATAATCATA TTGACCCCTG TGAGGAAGAC 
AAGGCCATGT CTTCTCTGAA ACAGCCCCAC TCCAAAGTTC TGGCCAAAGC AGTTGAGATT 
GTGCCCTTAC TGAAAAGCAC GAGGAATTTG GTTGTGAGTC TTAGTGGCAT TCTGCACAAG 
TTGGATCCAT ATGACTATGA AATTATTGAA GTTGTCTTGA AAATTATGCA AAAAGCTGAT 
GAAAAGGTCA CCAATCTCGA TATCAATCAG GGCTTGAGTC TGATGAAGCA TTTGAAATCT 
TATAGACGAG CATCTCCTCC TGGGAATCTG GAACATGAGT ACATCATGAA CCATACAATG 
ACCATGTCCT CAGCTGCTCA GATTCGGCTG CCTTTTCACC TGATTTTCTT TGGGAAAGTG 
CAGAACTTCT GGAAGATACT TTCTGCTGAG CTCCGGGAGG AGTCCTTCCC AACATTCCTG 
TTGATTGCTA AACTAATGAA GGTTTCTTTG GATTCTTTGT ATGTGTCGAC AGTTAAGCAA 
GTCTTTGAAA AAAGCTTGAA GCCAAAGTTG CTGAAGCAGG TGCAGGCTAA AGGCTCAACC 
CCGAATAACA AGGAAATAGC TAAAACCTTC CAGACTTTGC AGTCCTATTT ATTATCTATA 
GCTAATCCTG AATGGGCTGT TGCTGTTGCC ATCAATCTTG CTCAGGACAT TCCAGAAGGT 
TGCTACAAGA TAACTATATT GAAATTTTGC CTTTATTTGG CCGAGAAATG GTTAAAGAAT 
ATTTCATCCC AGGATGAAAC TCATGAGAAA GCTGAGGCCT TACTGAAGAA ACTGAGGGTG 
CAGTACCAGA GGTCAGGCAC TGAGACTGTG CTCATCACAC ATGGCCTGGG CTCTCCAGAG 
CATCTGAGAC TGCTAGGGAA GCCAGCACAA CTCATTGCCA GTCTATATGA GCATGGCAGC 
ATCCTGCCGA GGATCCAGGG ACCTGCTGAC AAAGACTGTC CTGATATTCA TGCAGCAGCC 
AAGGAAATAG CTGAAATTAA CAACTTGGAT TTGGAAAAAA TATGGGATGT GCTTCTTGAA 
AAATGGTTAT GTCCAGCGAC ACAGACCAAT GAAATACCTT CAGAATTCTT TAATCTTCAA 
GGGGATGAAG CCCTCCAAAG AGTTCTATAT CTCCTCCAAA TACGTCCCAT CGATTATAGT 
TCAAGGATGT TATTTTTGGT TGCCACATCT TCTGTATCTC CTTTTACTGT TGGTCAGCTG 
ACATTTGCCT ATAGAAGACG AGCTCTTCAC TGTCTCTTAT GCTTGGCCGA CAGAGAAACC 
ATTGAGTCTC TCTTCAAAAA GCCTTACGAG GAAGTAAAAT GTTTTCTGAA ATGCATCACT 
TTCCTGGCCG CATTTGAAAT GTTGAACATC CCTCTCACAT ATGAATCATT TCACAACAGT 
CCTAAAGAAG GAATGATTAA GGGCCTATGG AAAAATCATA GCCATGAACC TATGGCTGTG 
AGATTGGTGA CTGAACTTAG TTTGGAATAC AAGGTATATG ACACTCAGCT TTGGAGTGGG 
CTGTTACAAA AGCTGCTTGG CTTCAACATG ATTAGCTATC TGAGGAATGT TTTAACAGCA 
ATTTCCAATA TACATTCCTT GTGGCAGATC CCTTACTTTG GGAGAGCCTG GCAGCGGGTC 
TCACAGATAC CACTCATTTC AGCCTCTTGC CCCCTGAATC CTACTCAGCT GTTGGCTTGT 
CGTGAGAGTC TGGTGGTTCT TCTAGAGTGT CCTGTTTCCA TGGATCTTGA TATGATTGGA 
GTTGCCAAGC AGTTTAGCCA ACTGGAGCTT CCAGCCTTTG CCTTGGCCTG CCTTCTGCTG 
ATGCCCCACT CAGACAAGAG AGACCAGCAG CTTCAGAATT TCTTGGGATG GTGTAATCCT 
GAGGTTATTT TACAACAAAT AGATGAACAT ATGAATACTG GCCAGCTAGC AGGATTTGCC 
TGTCAAGTGA AGGGCTTTAT TCTGAATCAC ATCATAAATA AGAAGCAGTT TGGGATACTG 
GCAAACACAA AATACTTTCC ATTGTTGAAA TTACAAGTGA TAAGTACCCA GAAGGTCAAG 
GAGTTGGTGG ACTATCTAGT CAACAATGAC AGTTTGGATG AAGCCTCACT CCTTATAATG 
GAATACTTAA AGCAGTGTGG AAAAGCAGTG CCACTAGATA TCACTCCTTG TGACCTAGTG 
AAGATGTTTC TAAGTGAATC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KNTC1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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