Arfgef2 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgef2
Entrez Gene ID	101840187
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021229732.1	XP_021085391.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
XM_005074414.2	XP_005074471.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2
XM_005074414.3	XP_005074471.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following Arfgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe128116	XM_005074414.3 Latest version!	Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe250079	XM_021229732.1 Latest version!	Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMe128116	XM_005074414.2	Mesocricetus auratus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgef2 ( XM_005074414.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005074471.1 Mesocricetus auratus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.

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CloneID	OMe128116
Clone ID Related Accession (Same CDS sequence)	XM_005074414.2 , XM_005074414.3
Accession Version	XM_005074414.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801651.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005074414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACTGTTTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCTGAA AGCCATCGGG AAGCATGGAC GAATCTCCTG 
CTGTTACTTC TAACCAAAAC CCTCAAAATA AATGATGAAA AGTTCAAAGC CCACGCTTCG 
GTCTACTACC CCTACTTGTG TGAAATCATG CAATTCGACC TGATCCCTGA GCTCCGAGCA 
GTTCTTCGGA AGTTCTTCCT GAGGATAGGC CTGGTATATA AGATATGGAT TCCAGAGGAG 
CCATCACAAG TGCCAGCAGC ATTGTCATCG ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005074471.1
CDS	140..5518
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEVRELEKPIQSKPQSPVIQATAGSPKFSQLKQSQAQSKPTTPEKTDLPNGDHARSGLGKVSSENGEAHRERSSSLSGSAEHSRGTDDGAQEVVKDILEDVVTSAVKEAAEKHGLTEPDGVLSALECQECAVHPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAVRFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTTIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTDVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTIATKSAKQNVASEKQRRLLYNMEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFLGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMSRIRLQWSRIWHVIGEHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYISERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSAVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEESSDKHLDVDLDRQSLSSIDRNASERGQSQFSNPTDDSWKGAPYANQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDEDRRDSWNEIQQRLLTVCSEALAYFITVNSESHREAWTNLLLLLLTKTLKINDEKFKAHASVYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_005074414.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005074414.2

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACTGTTTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCTGAA AGCCATCGGG AAGCATGGAC GAATCTCCTG 
CTGTTACTTC TAACCAAAAC CCTCAAAATA AATGATGAAA AGTTCAAAGC CCACGCTTCG 
GTCTACTACC CCTACTTGTG TGAAATCATG CAATTCGACC TGATCCCTGA GCTCCGAGCA 
GTTCTTCGGA AGTTCTTCCT GAGGATAGGC CTGGTATATA AGATATGGAT TCCAGAGGAG 
CCATCACAAG TGCCAGCAGC ATTGTCATCG ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_005074414.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005074471.1 Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X1, mRNA.

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CloneID	OMe128116
Clone ID Related Accession (Same CDS sequence)	XM_005074414.2 , XM_005074414.3
Accession Version	XM_005074414.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801651.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005074414.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACTGTTTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCTGAA AGCCATCGGG AAGCATGGAC GAATCTCCTG 
CTGTTACTTC TAACCAAAAC CCTCAAAATA AATGATGAAA AGTTCAAAGC CCACGCTTCG 
GTCTACTACC CCTACTTGTG TGAAATCATG CAATTCGACC TGATCCCTGA GCTCCGAGCA 
GTTCTTCGGA AGTTCTTCCT GAGGATAGGC CTGGTATATA AGATATGGAT TCCAGAGGAG 
CCATCACAAG TGCCAGCAGC ATTGTCATCG ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005074471.1
CDS	149..5527
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEVRELEKPIQSKPQSPVIQATAGSPKFSQLKQSQAQSKPTTPEKTDLPNGDHARSGLGKVSSENGEAHRERSSSLSGSAEHSRGTDDGAQEVVKDILEDVVTSAVKEAAEKHGLTEPDGVLSALECQECAVHPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAVRFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTTIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTDVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTIATKSAKQNVASEKQRRLLYNMEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFLGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMSRIRLQWSRIWHVIGEHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYISERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSAVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEESSDKHLDVDLDRQSLSSIDRNASERGQSQFSNPTDDSWKGAPYANQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDEDRRDSWNEIQQRLLTVCSEALAYFITVNSESHREAWTNLLLLLLTKTLKINDEKFKAHASVYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW

Target ORF information:

RefSeq Version	XM_005074414.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005074414.3

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACTGTTTG CAGTGAAGCA 
CTTGCCTATT TCATTACTGT GAACTCTGAA AGCCATCGGG AAGCATGGAC GAATCTCCTG 
CTGTTACTTC TAACCAAAAC CCTCAAAATA AATGATGAAA AGTTCAAAGC CCACGCTTCG 
GTCTACTACC CCTACTTGTG TGAAATCATG CAATTCGACC TGATCCCTGA GCTCCGAGCA 
GTTCTTCGGA AGTTCTTCCT GAGGATAGGC CTGGTATATA AGATATGGAT TCCAGAGGAG 
CCATCACAAG TGCCAGCAGC ATTGTCATCG ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef2 ( XM_021229732.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021085391.1 Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X2, mRNA.

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CloneID	OMe250079
Clone ID Related Accession (Same CDS sequence)	XM_021229732.1
Accession Version	XM_021229732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801651.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021085391.1
CDS	149..5239
Translation	MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEVRELEKPIQSKPQSPVIQATAGSPKFSQLKQSQAQSKPTTPEKTDLPNGDHARSGLGKVSSENGEAHRERSSSLSGSAEHSRGTDDGAQEVVKDILEDVVTSAVKEAAEKHGLTEPDGVLSALECQECAVHPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAVRFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTTIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTDVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTIATKSAKQNVASEKQRRLLYNMEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFLGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMSRIRLQWSRIWHVIGEHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYISERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSAVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEESSDKHLDVDLDRQSLSSIDRNASERGQSQFSNPTDDSWKGAPYANQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDEDRRDSWNEIQQRLLTV

Target ORF information:

RefSeq Version	XM_021229732.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus ADP ribosylation factor guanine nucleotide exchange factor 2 (Arfgef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021229732.1

ORF Insert Sequence:

ATGCAGGAAA GCCAGACCAA GAGCATGTTC GTGTCCCGGG CCCTGGAGAA GATCCTAGCC 
GACAAGGAGG TGAAGCGGCC CCAGCACTCA CAGCTGCGTA GGGCCTGCCA GGTGGCGCTC 
GATGAAATTA AAGCAGAGAT AGAAAAGCAG AGGCTTGGTG CTGCGGCTCC GCCAAAGGCA 
AACTTCATTG AAGCTGACAA GTATTTCCTT CCATTCGAGC TGGCCTGCCA GTCCAAGTCC 
CCCCGGGTGG TCAGCACATC TCTGGATTGC TTGCAGAAAC TCATCGCATA TGGGCACATC 
ACTGGCAACG CCCCAGACAG TGGAGCTCCT GGGAAGCGGC TAATCGACAG GATTGTTGAA 
ACCATTTGCA ATTGTTTTCA GGGCCCTCAG ACGGATGAAG GGGTTCAGTT ACAAATAATT 
AAGGCTCTGC TGACAGCAGT GACTTCCCCA CACATTGAAA TCCACGAGGG CACGATCCTA 
CAGACAGTGA GGACATGCTA CAACATCTAT CTGGCCAGCA AAAACCTCAT CAATCAGACC 
ACAGCCAAGG CCACGCTTAC CCAGATGCTG AATGTCATCT TTACCCGCAT GGAAAACCAA 
GTGTTACAGG AAGTCAGAGA GTTGGAAAAA CCAATCCAGT CTAAACCTCA GTCCCCTGTG 
ATCCAGGCCA CAGCAGGATC TCCCAAATTC AGCCAATTGA AACAGAGCCA GGCCCAAAGT 
AAACCAACGA CGCCTGAAAA AACAGACTTG CCCAACGGTG ATCATGCCAG GAGTGGCCTA 
GGAAAAGTGA GCTCAGAAAA TGGAGAGGCT CACCGAGAAA GAAGCTCATC GCTCTCTGGT 
TCAGCTGAGC ATTCCAGGGG CACTGATGAC GGAGCCCAGG AGGTGGTGAA GGACATCTTA 
GAGGATGTGG TTACATCTGC TGTGAAAGAA GCAGCAGAAA AGCATGGTCT GACAGAGCCG 
GACGGAGTCC TGAGTGCCCT AGAGTGCCAG GAATGCGCTG TTCACCCAGG AGTTGACGAA 
AACTCACAGA CCAATGGCAT AGCCGACGAC AGGCAGTCAC TGTCCTCAGC AGATAATCTG 
GAACCAGATG TGCAAGGACA TCAAGTGGCT GTTAGGTTCT CGCACATTCT ACAGAAGGAT 
GCCTTCCTGG TGTTCCGCTC CCTGTGCAAG CTGTCCATGA AGCCTCTCGG GGAAGGACCT 
CCAGATCCAA AGTCTCACGA ACTTCGTTCC AAAGTGGTTT CCCTGCAGCT GCTGCTCTCC 
GTGCTGCAGA ATGCTGGCCC AGTCTTCAGG AGCCATGAGA TGTTTGTCAC TGCCATTAAG 
CAGTACCTGT GTGTGGCCCT GTCCAAGAAT GGCGTGTCCT CGGTGCCTGA TGTCTTCGAG 
CTCTCACTTG CTATTTTTCT TACTCTTCTT TCAAACTTCA AGATGCACTT GAAAATGCAG 
ATAGAGGTCT TTTTCAAAGA GATTTTCCTG AACATCTTAG AAACATCCAC AAGTTCTTTC 
GAGCACCGCT GGATGGTTAT CCAGACCCTG ACGAGGATTT GTGCAGATGC CCAATGTGTT 
GTGGATATTT ATGTCAACTA TGACTGTGAC TTAAATGCTG CTAACATTTT CGAGCGTCTA 
GTAAATGATT TATCCAAAAT CGCTCAGGGA AGAAGTGGAC ATGAGCTGGG CATGACGCCT 
CTACAGGAGC TCAGTCTGAG GAAGAAAGGC CTGGAGTGTC TCGTGTCCAT TCTCAAGTGC 
ATGGTGGAAT GGAGCAAGGA CCTATATGTG AACCCCAACC ACCAGGCCAC CCTGGGTCAG 
GAGAGGCTCC CAGATCAGGA AATGGGGGAC GGAAAAGGCC TTGACATGGC AAGACGATGC 
AGTGTGACAT CAGTGGAGTC CACAGTGTCC TCAGGGACCC AGACAACCAT TCAGGATGAC 
CCAGAGCAGT TTGAGGTCAT CAAACAACAG AAGGAGATCA TTGAGCATGG CATCGAGCTC 
TTCAACAAGA AGCCCAAGCG GGGAATCCAG TTTCTCCAAG AGCAAGGCAT GTTGGGAACA 
GACGTAGAGG ACATTGCCCA GTTTCTGCAC CAGGAGGAAC GGCTGGATTC TACCCAGGTT 
GGTGAATTTC TGGGTGATAG CACGAGGTTC AACAAGGAAG TGATGTATGC CTATGTGGAC 
CAACTGGACT TCTGTGAAAA AGAGTTCGTC TCAGCCCTGA GGACATTCCT GGAAGGCTTC 
CGCCTGCCTG GAGAAGCGCA GAAAATTGAC CGGTTAATGG AGAAGTTTGC TGCTCGATAC 
ATAGAGTGTA ACCAAGGGCA AACCCTGTTT GCTAGTGCTG ACACTGCGTA CGTCCTGGCA 
TACTCCATCA TTATGCTAAC TACAGACTTG CACAGCCCTC AGGTAAAGAA TAAAATGACA 
AAAGAGCAAT ATATTAAAAT GAATCGGGGT ATCAATGATA GTAAAGACCT CCCAGAGGAG 
TATCTGTCCA GCATCTACGA AGAAATAGAA GGAAAGAAGA TTGCAATGAA GGAGACGAAA 
GAACACACAA TTGCAACCAA ATCTGCCAAG CAGAATGTAG CTAGTGAAAA GCAGCGGCGA 
CTGCTGTACA ATATGGAAAT GGAACAGATG GCTAAAACGG CCAAAGCTCT GATGGAGGCT 
GTGAGCCATG CCAAAGCTCC GTTTACCAGT GCCACACACT TGGACCATGT CCGGCCAATG 
TTCAAATTGG TGTGGACGCC GCTGCTGGCA GCCTATAGCA TTGGCCTGCA GAACTGTGAT 
GACACAGAGG TGGCCTCCCT GTGTTTGGAA GGCATCAGGT GTGCAGTCCG CATCGCCTGC 
ATCTTTGGAA TGCAGCTGGA ACGAGATGCC TATGTTCAGG CCCTTGCTCG CTTTTCCCTG 
CTGACAGCCA GCTCCAGCAT CACAGAAATG AAGCAGAAGA ACATCGACAC CATAAAGACG 
CTCATCACCG TGGCTCACAC TGATGGCAAC TACCTGGGCA ACTCTTGGCA TGAGATCTTG 
AAATGCATCA GCCAGCTGGA GCTGGCTCAG CTGATAGGAA CTGGGGTGAA GACTCGCTAC 
CTGTCTGGTT CTGGACGGGA AAGAGAAGGG AGCCTGAAGG GCCACACGTT GGCAGGAGAA 
GAATTCCTGG GTCTTGGCCT CGGTAATTTG GTGAGTGGTG GTGTAGACAA AAGACAGATG 
GCCAGCTTCC AGGAGTCTGT TGGTGAAACC AGCTCACAGA GTGTGGTGGT TGCAGTGGAC 
AGAATTTTTA CTGGCTCTAC TAGACTGGAT GGAAATGCAA TAGTTGACTT TGTCCGCTGG 
CTTTGTGCTG TGTCTATGGA TGAGCTGGCC TCTCCCCACC ATCCCCGGAT GTTCAGCCTG 
CAGAAGATTG TGGAGATATC GTACTATAAC ATGAGCCGGA TCCGGCTACA GTGGTCTCGG 
ATATGGCATG TCATTGGAGA GCACTTCAAT AAGGTTGGCT GTAACCCCAA TGAAGATGTG 
GCTATCTTTG CTGTTGATTC ACTGAGGCAA CTCTCCATGA AGTTTCTGGA GAAGGGAGAA 
TTGGCCAACT TCAGATTTCA GAAGGATTTT CTGAGGCCAT TTGAGCATAT TATGAAGAAA 
AACAGGTCCC CGACCATCCG GGACATGGTG ATCCGCTGCA TTGCCCAGAT GGTGAGCTCC 
CAGGCAGCCA ACATCCGCTC TGGCTGGAAG AACATCTTTG CTGTGTTCCA CCAGGCAGCC 
TCTGACCACG ATGGGAACAT TGTGGAACTG GCCTTCCAGA CCACAGGCCA CATCGTCTCA 
ACCATTTTCC AGCACCATTT CCCTGCAGCC ATCGACTCCT TCCAGGATGC TGTGAAGTGC 
TTGTCAGAGT TTGCCTGCAA TGCCGCTTTC CCTGACACCA GCATGGAGGC CATCAGGCTC 
ATCCGCTTCT GTGGGAAATA CATCTCAGAG AGGCCTCGGG TACTGCAAGA ATACACAAGC 
GATGACATGA ATGTGGCTCC TGGTGACAGA GTCTGGGTCA GAGGCTGGTT TCCCATCCTC 
TTTGAGCTCT CTTGCATCAT TAACAGATGC AAGTTAGATG TACGCACAAG GGGACTAACC 
GTCATGTTTG AGATCATGAA GAGCTATGGC CACACCTTTG CAAAGCACTG GTGGCAGGAC 
CTGTTCAGAA TCGTGTTTCG AATTTTTGAC AACATGAAAC TCCCCGAGCA ACAGTCGGAG 
AAGTCAGAGT GGATGACGAC CACCTGCAAT CACGCACTGT ATGCTATCTG TGACGTGTTC 
ACCCAGTTCT ACGAGGCTTT AAATGAAGTG CTTCTCTCCG CTGTGTTTGC ACAGCTGCAG 
TGGTGCGTGA AACAAGATAA TGAGCAGTTG GCGCGATCAG GTACTAATTG CTTAGAGAAC 
CTAGTGATAT CCAATGGAGA GAAGTTCAGC CCTGCTGTTT GGGATGAAAC CTGCAATTGC 
ATGTTGGATA TTTTCAAAAC CACCATTCCA CATGTTTTGC TGACGTGGAG ACCTGCAGGG 
ATGGAGGAGG AGTCATCAGA TAAACATTTG GATGTGGACC TGGACCGCCA GTCCTTAAGT 
AGTATAGACA GAAATGCCTC GGAGAGAGGA CAGAGCCAGT TCTCCAACCC CACTGACGAC 
AGCTGGAAGG GTGCACCATA TGCAAATCAG AAACTGTTGG CCAGCCTGCT CATCAAGTGC 
GTGGTTCAGC TGGAGTTGAT ACAGACCATT GACAACATCG TATTCTACCC TGCAACCAGC 
AAAAAGGAGG ATGCGGAGCA CATGGTTGCT GCCCAGCAAG ACACACTAGA TGCAGATATC 
CACATCGAGA CGGAGAATCA GGGCATGTAC AAGTTCATGT CTTCCCAGCA CCTCTTCAAG 
CTGTTAGACT GCCTGCAGGA GTCCCATTCG TTCTCCAAGG CCTTCAACTC CAACTATGAG 
CAGAGAACTG TCCTCTGGAG AGCAGGCTTC AAGGGCAAGT CCAAACCCAA TCTTCTCAAA 
CAAGAAACCA GCAGCCTGGC CTGTTGTTTG AGGATCCTGT TTAGAATGTA TGTCGATGAG 
GACCGCAGGG ATTCCTGGAA TGAAATACAG CAGCGACTTC TAACGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Arfgef2 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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