NIPBL cDNA ORF clone, Mesocricetus auratus(Golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIPBL
Entrez Gene ID	101836378
Full Name	NIPBL, cohesin loading factor
Gene Type	protein-coding
Organism	Mesocricetus auratus(Golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005077992.2	XP_005078049.1	nipped-B-like protein isoform X1
XM_005077992.3	XP_005078049.1	nipped-B-like protein isoform X1
XM_013119043.2	XP_012974497.1	nipped-B-like protein isoform X2
XM_013119043.1	XP_012974497.1	nipped-B-like protein isoform X2

Pathways from BioSystems
Gene Ontology

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe131786	XM_005077992.3 Latest version!	Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMe131787	XM_013119043.2 Latest version!	Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMe131786	XM_005077992.2	Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMe131787	XM_013119043.1	Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIPBL ( XM_005077992.2 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_005078049.1 Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X1, mRNA.

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CloneID	OMe131786
Clone ID Related Accession (Same CDS sequence)	XM_005077992.2 , XM_005077992.3
Accession Version	XM_005077992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	nipped-B-like protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801682.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005077992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGTCATTG 
AGAAGGTCAA AACGAAATTC AGACTCTACG GAGTTGGCAG CACAGATGAA TGAAAGTGTT 
GACGTCATGG ATGTCATCGC TATTTGCTGT CCAAAGTACA AAGATCGACC ACAAATTGCA 
AGAGTAGTAC AGAAAACCAG CAGTGGCTTC AGTGTTCAGT GGATGGCAGG CTCCTACAGT 
GGCTCCTGGA CAGAGGCTAA GCGCCGCGAT GGCCGCAAAC TGGTGCCTTG GGTAGACACT 
ATTAAAGAGT CAGACATTAT TTACAAAAAA ATTGCTCTAA CGAGTGCTAA TAAGCTGACT 
AATAAAGTTG TTCAGACTTT ACGATCCCTG TATGCCGCCA AGGACGGGAC TTCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005078049.1
CDS	412..8811
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNSRIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPTKDSTKLTLRLSRVKSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEMKQCNDAPISVLQEDIIGSLKSIPENHPETPKKKSEPELSKSEVKQNESRLSESKPNENQLLETKSSERVLETKTETQTEELKQNENKTTECKQNESTVVEPKQSENRLCDAKPNDNKQNNTRSETTKSRPETPKQKAESRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGESRPETPKHRHENRRDAGKPSTEKKPDVSKHKQDIKSDSPRLKSERAEALKQRPDGRSESLRRDHDSKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHSIKSDGSKTEKLERKHRHESGDSRDRLSSGEQKSRPDSPRVKQGDTNKSRPGFKSPNSKDDKRTEGNRSKVDSNKAHTDSKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMDMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREEKSRSSLKSIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDVDGEPMYIQMVTALVLQLIQCVVHLPSSEKDPNSEEDSNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSDGAHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYTMIRGNRQHRRAFLISLLNLFDDTAKTEVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKTSPAKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDVNSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKLTEDVKRSIVRQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_005077992.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005077992.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGTCATTG 
AGAAGGTCAA AACGAAATTC AGACTCTACG GAGTTGGCAG CACAGATGAA TGAAAGTGTT 
GACGTCATGG ATGTCATCGC TATTTGCTGT CCAAAGTACA AAGATCGACC ACAAATTGCA 
AGAGTAGTAC AGAAAACCAG CAGTGGCTTC AGTGTTCAGT GGATGGCAGG CTCCTACAGT 
GGCTCCTGGA CAGAGGCTAA GCGCCGCGAT GGCCGCAAAC TGGTGCCTTG GGTAGACACT 
ATTAAAGAGT CAGACATTAT TTACAAAAAA ATTGCTCTAA CGAGTGCTAA TAAGCTGACT 
AATAAAGTTG TTCAGACTTT ACGATCCCTG TATGCCGCCA AGGACGGGAC TTCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005077992.3 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_005078049.1 Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X1, mRNA.

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CloneID	OMe131786
Clone ID Related Accession (Same CDS sequence)	XM_005077992.2 , XM_005077992.3
Accession Version	XM_005077992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	nipped-B-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801682.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005077992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGTCATTG 
AGAAGGTCAA AACGAAATTC AGACTCTACG GAGTTGGCAG CACAGATGAA TGAAAGTGTT 
GACGTCATGG ATGTCATCGC TATTTGCTGT CCAAAGTACA AAGATCGACC ACAAATTGCA 
AGAGTAGTAC AGAAAACCAG CAGTGGCTTC AGTGTTCAGT GGATGGCAGG CTCCTACAGT 
GGCTCCTGGA CAGAGGCTAA GCGCCGCGAT GGCCGCAAAC TGGTGCCTTG GGTAGACACT 
ATTAAAGAGT CAGACATTAT TTACAAAAAA ATTGCTCTAA CGAGTGCTAA TAAGCTGACT 
AATAAAGTTG TTCAGACTTT ACGATCCCTG TATGCCGCCA AGGACGGGAC TTCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005078049.1
CDS	417..8816
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNSRIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPTKDSTKLTLRLSRVKSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEMKQCNDAPISVLQEDIIGSLKSIPENHPETPKKKSEPELSKSEVKQNESRLSESKPNENQLLETKSSERVLETKTETQTEELKQNENKTTECKQNESTVVEPKQSENRLCDAKPNDNKQNNTRSETTKSRPETPKQKAESRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGESRPETPKHRHENRRDAGKPSTEKKPDVSKHKQDIKSDSPRLKSERAEALKQRPDGRSESLRRDHDSKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHSIKSDGSKTEKLERKHRHESGDSRDRLSSGEQKSRPDSPRVKQGDTNKSRPGFKSPNSKDDKRTEGNRSKVDSNKAHTDSKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMDMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREEKSRSSLKSIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDVDGEPMYIQMVTALVLQLIQCVVHLPSSEKDPNSEEDSNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSDGAHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYTMIRGNRQHRRAFLISLLNLFDDTAKTEVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKTSPAKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDVNSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKLTEDVKRSIVRQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_005077992.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005077992.3

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGTCATTG 
AGAAGGTCAA AACGAAATTC AGACTCTACG GAGTTGGCAG CACAGATGAA TGAAAGTGTT 
GACGTCATGG ATGTCATCGC TATTTGCTGT CCAAAGTACA AAGATCGACC ACAAATTGCA 
AGAGTAGTAC AGAAAACCAG CAGTGGCTTC AGTGTTCAGT GGATGGCAGG CTCCTACAGT 
GGCTCCTGGA CAGAGGCTAA GCGCCGCGAT GGCCGCAAAC TGGTGCCTTG GGTAGACACT 
ATTAAAGAGT CAGACATTAT TTACAAAAAA ATTGCTCTAA CGAGTGCTAA TAAGCTGACT 
AATAAAGTTG TTCAGACTTT ACGATCCCTG TATGCCGCCA AGGACGGGAC TTCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_013119043.2 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012974497.1 Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X2, mRNA.

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CloneID	OMe131787
Clone ID Related Accession (Same CDS sequence)	XM_013119043.2 , XM_013119043.1
Accession Version	XM_013119043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	nipped-B-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801682.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013119043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGGTGAGG 
CGGAGGAGGA GTCAACGTAT TTCCCAGCGT ATTACTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012974497.1
CDS	417..8498
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNSRIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPTKDSTKLTLRLSRVKSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEMKQCNDAPISVLQEDIIGSLKSIPENHPETPKKKSEPELSKSEVKQNESRLSESKPNENQLLETKSSERVLETKTETQTEELKQNENKTTECKQNESTVVEPKQSENRLCDAKPNDNKQNNTRSETTKSRPETPKQKAESRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGESRPETPKHRHENRRDAGKPSTEKKPDVSKHKQDIKSDSPRLKSERAEALKQRPDGRSESLRRDHDSKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHSIKSDGSKTEKLERKHRHESGDSRDRLSSGEQKSRPDSPRVKQGDTNKSRPGFKSPNSKDDKRTEGNRSKVDSNKAHTDSKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMDMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREEKSRSSLKSIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDVDGEPMYIQMVTALVLQLIQCVVHLPSSEKDPNSEEDSNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSDGAHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYTMIRGNRQHRRAFLISLLNLFDDTAKTEVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKTSPAKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDVNSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKLTEDVKRSIVRQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_013119043.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus NIPBL, cohesin loading factor (Nipbl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013119043.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGGTGAGG 
CGGAGGAGGA GTCAACGTAT TTCCCAGCGT ATTACTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_013119043.1 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012974497.1 Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X2, mRNA.

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CloneID	OMe131787
Clone ID Related Accession (Same CDS sequence)	XM_013119043.2 , XM_013119043.1
Accession Version	XM_013119043.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	nipped-B-like protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801682.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGGTGAGG 
CGGAGGAGGA GTCAACGTAT TTCCCAGCGT ATTACTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012974497.1
CDS	412..8493
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNSRIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPTKDSTKLTLRLSRVKSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEMKQCNDAPISVLQEDIIGSLKSIPENHPETPKKKSEPELSKSEVKQNESRLSESKPNENQLLETKSSERVLETKTETQTEELKQNENKTTECKQNESTVVEPKQSENRLCDAKPNDNKQNNTRSETTKSRPETPKQKAESRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGESRPETPKHRHENRRDAGKPSTEKKPDVSKHKQDIKSDSPRLKSERAEALKQRPDGRSESLRRDHDSKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHSIKSDGSKTEKLERKHRHESGDSRDRLSSGEQKSRPDSPRVKQGDTNKSRPGFKSPNSKDDKRTEGNRSKVDSNKAHTDSKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMDMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREEKSRSSLKSIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDVDGEPMYIQMVTALVLQLIQCVVHLPSSEKDPNSEEDSNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSDGAHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYTMIRGNRQHRRAFLISLLNLFDDTAKTEVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKTSPAKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDVNSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKLTEDVKRSIVRQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_013119043.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus Nipped-B homolog (Drosophila) (Nipbl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013119043.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATA ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTC 
CTCTTTAATT CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCCTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGTCTCAAC CAGGTTTCCA CAGATCACAT AGAACTGAAA 
GATAACCTTG GCAGTGATGA CCCGGAAGGT GATATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTACCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCTATT GTTGCCGGTG GCTTGAGAAA CATCCATGAT 
AACAAAGTTT CTGGTCCATT ATCTGGCAAC TCAGCTAATC ATCATGCTGA TAACCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGATGGCGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGGT CTCCACAGCC AGTTTGTTCC 
CCTGCTGGAA GTGATGGAAC ACCTAAAGGA TCAAGACCAC CTTTAATTCT ACAATCTCAG 
TCTTTACCTT GTTCATCACC TCGAGATGTT CCTCCAGATA TCTTGCTAGA TTCTCCAGAG 
AGAAAACAAA AAAAGCAAAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA CCAGAATGAG 
AAAGCTGCAA TGTATGATAT AATTAGTTCT CCAACCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTTCTC GTGTAAAATC TTCAGATATG GACCAGCAAG AGGATATGCT ATCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTATCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGACATA AACCGCCCAC TTAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCACAGACT TCTGTAGTAC AGAATCAGCA ACAGGTATCA 
CAGCAGGGGC CGATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGGATA 
GAGAGAGAAT CAGCCATTGA AAGGGAGCGC TTCTCAAAGG AAGTTCAAGA TAAAGATAAA 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATG AAACAGTGTA ATGATGCACC CATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAGTCTTAAG TCTATACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAAT CTGAACCTGA GCTCTCAAAA AGTGAAGTGA AACAAAATGA AAGTAGGTTG 
TCAGAATCTA AACCAAATGA AAACCAACTG CTGGAGACAA AGTCAAGTGA AAGAGTGTTA 
GAAACTAAAA CCGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAACAA AACTACAGAG 
TGCAAACAGA ATGAGAGCAC TGTAGTTGAG CCTAAACAGA GTGAAAATAG ACTATGTGAC 
GCAAAACCAA ATGACAACAA ACAAAACAAT ACCAGGTCAG AAACAACAAA ATCAAGACCT 
GAAACCCCAA AGCAGAAAGC TGAAAGCCGG CCTGAAACTC CAAAGCAAAA GGGTGAAGGT 
CGACCTGAAA CCCCAAAACA GAAGGGTGAA GGCCGGCCTG AAACCCCAAA GCAAAAGGGT 
GAAGGCCGGC CTGAAACCCC AAAGCAGAAA GGTGAAAGCC GGCCTGAGAC CCCAAAGCAC 
AGGCATGAAA ATAGGAGGGA TGCTGGAAAG CCATCAACAG AGAAAAAACC TGATGTGTCT 
AAGCATAAAC AGGATATCAA ATCTGACTCA CCTCGCTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAAA GACCTGATGG GCGATCTGAG TCACTAAGAC GTGACCATGA TAGCAAACAG 
AAATCAGATG ATAGGGGTGA GTCAGAACGA CATCGGGGGG ATCAGTCAAG GGTTCGAAGA 
CCAGAAACAT TGAGATCTTC TAGTAGAAAT GAACATAGCA TTAAATCGGA TGGTTCAAAA 
ACTGAAAAGC TAGAAAGAAA ACACAGGCAT GAATCAGGGG ACTCACGGGA CAGACTTTCT 
TCTGGGGAAC AAAAATCAAG ACCAGACAGT CCTCGTGTAA AACAAGGAGA TACTAATAAA 
TCAAGACCTG GTTTTAAATC ACCAAATAGT AAAGACGACA AAAGGACAGA GGGTAACAGA 
AGTAAAGTAG ACAGTAATAA AGCGCACACT GACAGTAAAG CTGAATTTCC AAGCTATTTG 
TTAGGGGGCA GGTCTGGTGC ATTGAAAAAT TTCGTCATTC CAAAAATCAA GAGGGATAAA 
GATGGCAATA TTACTCAGGA AACTAAGAAA ATGGACATGA AAGGAGAGCA GAAAGACAAA 
GTAGAAAAAA TGGGATTAGT TGAAGATCTA AACAAAGGCG CTAAGCCCGT AGTTGTTCTG 
CAAAAACTGT CGCTGGATGA TGTCCAGAAG CTAATTAAAG ATAGAGAGGA GAAATCAAGA 
AGTTCCCTTA AGTCTATCAA GAATAAGCCA TCCAAGTCAA ATAAAGGTAG TATAGATCAA 
TCAGTGTTAA AAGAATTGCC CCCTGAACTC CTGGCAGAAA TTGAGTCTAC CATGCCACTT 
TGTGAACGTG TGAAAATGAA CAAGCGCAAG CGTAGCACAG TTAATGAAAA GCCAAAATAT 
GCTGAAATCA GTTCAGATGA AGATAATGAT AGTGATGAAG CTTTTGAATC CTCTAGGAAA 
CGGCATAAAA AAGATGACGA TAAAGCTTGG GAATACGAAG AGCGTGACAG AAGAAGCTCT 
GGGGATCATA GGAGAAGTGG CCACTCACAT GAAGGAAGAA GGAGCTCGGG TGGTGGTCGT 
TACCGAAACC GAAGTCCATC AGATTCTGAT ATGGAAGATT ACTCTCCTCC TCCCAGCCTT 
AGTGAGGTTG CTAGAAAAAT GAAGAAAAAG GAAAAACAAA AGAAAAGAAA AGCTTATGAG 
CCGAAACTAA CACCTGAAGA AATGATGGAT TCTTCAACTT TTAAGAGATT TACAGCCTCA 
ATAGAAAATA TTTTGGATAA TTTAGAAGAT ATGGATTTTA CTGCATTTGG TGATGACGAT 
GAAATTCCTC AGGAACTGCT GTTAGGAAAA CATCAGCTTA ATGAACTTGG GAGTGAATCT 
GCCAAAATTA AAGCCATGGG TATAATGGAC AAGCTTTCAA CTGACAAAAC TGTGAAAGTC 
CTAAATATAT TGGAGAAGAA TATTCAGGAT GGATCAAAGC TTTCCACTTT GTTAAATCAC 
AATAATGATA CTGAAGAAGA AGAAAGATTG TGGAGAGATC TTATTATGGA GAGAGTTACA 
AAATCAGCAG ATGCCTGTCT TACAACTATC AACATTATGA CATCTCCTAA CATGCCAAAA 
GCTGTATACA TTGAAGATGT GATTGAAAGA GTTATACAGT ACACTAAATT TCACTTACAG 
AATACACTCT ATCCCCAGTA TGACCCTGTT TACAGATTAG ATCCACATGG AGGAGGGTTA 
TTGAGTTCAA AGGCCAAGCG GGCTAAGTGT TCTACTCATA AGCAGAGAGT GATAGTGATG 
CTTTATAACA AAGTGTGTGA CATTGTCAGC AGTTTATCAG AATTGCTAGA GATACAGCTT 
CTTACAGACA CGACGATCCT TCAGGTTTCA TCAATGGGAA TAACACCATT TTTTGTAGAA 
AATGTCAGTG AACTACAATT GTGTGCCATT AAGTTAGTCA CTGCAGTGTT CTCAAGATAT 
GAAAAGCATA GGCAATTAAT ATTGGAAGAA ATTTTTACTT CACTTGCAAG ATTACCAACT 
AGCAAGAGAA GTTTAAGGAA TTTCAGGTTA AATAGTAGTG ATGTGGATGG AGAGCCAATG 
TATATCCAGA TGGTTACAGC ACTAGTTTTA CAACTTATTC AGTGTGTGGT TCACTTACCC 
TCATCAGAAA AGGACCCTAA TTCAGAAGAA GACTCAAACA AAAAGGTTGA CCAGGATGTT 
GTCATTACTA ACTCTTATGA AACAGCTATG AGAACAGCCC AAAATTTTCT TTCCATCTTC 
CTTAAAAAAT GTGGTAGTAA ACAAGGTGAA GAAGATTACA GACCACTTTT TGAAAATTTT 
GTTCAAGACC TTCTTTCAAC AGTCAATAAG CCTGAGTGGC CAGCTGCTGA ACTACTCCTT 
AGTTTGTTAG GGAGACTGTT GGTGCATCAA TTTAGTAATA AGTCAACAGA AATGGCTTTA 
AGAGTGGCAT CTCTTGATTA CCTTGGAACT GTTGCTGCAC GACTGAGAAA AGATGCTGTT 
ACCAGCAAGA TGGATCAAGG GTCTATAGAA CGAATTTTAA AACAGGTTTC TGGAGGAGAA 
GATGAAATCC AACAATTGCA AAAGGCATTG CTTGACTATT TGGATGAGAA CACTGAGACT 
GACCCTTCAC TGGTGTTTTC TCGTAAATTC TATATAGCCC AATGGTTCCG TGACACAACT 
TTGGAAACAG AAAAAGCAAT GAAATCACAA AAAGATGAAG AATCATCTGA TGGAGCACAT 
CATGCAAAAG AAATTGAGAC AACGGGTCAG ATAATGCATC GAGCTGAAAA CCGGAAAAAG 
TTCCTTAGAA GCATTATCAA AACCACACCG TCCCAGTTCA GCACATTGAA GATGAACTCG 
GACACTGTGG ACTATGATGA TGCGTGCTTG ATTGTTCGAT ACTTGGCCTC TATGAGGCCG 
TTTGCCCAGA GCTTTGATAT TTATTTGACA CAGATACTAC GAGTTCTTGG TGAAAATGCA 
ATTGCTGTTC GAACAAAAGC CATGAAGTGT TTATCTGAGG TTGTTGCTGT AGACCCCAGT 
ATTCTAGCAA GGCTTGACAT GCAGCGAGGA GTTCATGGAC GATTAATGGA TAACTCCACT 
AGCGTTCGAG AGGCAGCCGT GGAATTACTA GGTCGATTTG TCCTTTGCCG ACCTCAGCTT 
GCTGAGCAGT ATTATGATAT GCTGATTGAA AGAATATTGG ATACTGGTAT CAGTGTCAGG 
AAAAGGGTAA TAAAGATTTT AAGAGACATC TGTATTGAAC AACCAACATT TCCAAAAATT 
ACAGAAATGT GTGTAAAAAT GATTCGTAGA GTCAATGATG AAGAGGGCAT TAAGAAATTA 
GTAAATGAAA CATTCCAGAA ACTCTGGTTT ACTCCAACGC CACATAATGA CAAAGAAGCA 
ATGACAAGAA AAATCTTAAA CATCACTGAT GTGGTTGCAG CATGTAGAGA TACTGGATAT 
GACTGGTTTG AGCAACTCCT TCAAAATTTG TTGAAGTCTG AAGAGGATTC CTCATATAAA 
CCTGTGAAGA AAGCTTGCAC TCAGCTTGTT GATAACCTAG TTGAACATAT TCTTAAATAT 
GAGGAATCTC TAGCTGACTC AGACAATAAG GGTGTGAATT CTGGAAGATT GGTAGCTTGT 
ATAACCACTT TGTTCTTATT CAGCAAAATA AGACCCCAAC TCATGGTTAA ACATGCCATG 
ACTATGCAGC CATACCTTAC CACTAAATGT AGTACACAAA ATGATTTCAT GGTTATCTGC 
AATGTTGCAA AAATCCTTGA GTTGGTTGTA CCATTGATGG AACACCCAAG TGAAACTTTC 
CTTGCCACTA TTGAAGAAGA TCTGATGAAA CTGATCATCA AATATGGCAT GACTGTAGTA 
CAGCATTGTG TGAGCTGTCT TGGGGCTGTT GTAAATAAAG TGACACAGAA TTTTAAATTT 
GTGTGGGCCT GTTTCAATAG ATACTATGGT GCCATTTCAA AATTAAAAAG TCAGCACCAA 
GAAGACCCAA ATAATACTTC ACTTTTAACA AATAAACCAG CACTTCTTAG GTCCCTCTTC 
ACTGTTGGAG CACTCTGTCG GCATTTTGAT TTTGATCTGG AAGATTTTAA AGGCAACAGT 
AAGGTTAACA TAAAGGATAA AGTACTTGAG CTGTTGATGT ATTTTACGAA ACATTCAGAT 
GAAGAAGTAC AAACAAAAGC CATCATTGGT CTAGGCTTTG CCTTTATACA GCATCCAAGT 
CTAATGTTTG AGCAAGAAGT GAAGAATCTG TATAATAATA TTTTATCTGA TAAGAACTCC 
TCAGTGAATT TAAAAATACA AGTGTTAAAA AACCTGCAGA CTTACCTACA AGAAGAAGAC 
ACACGAATGC AGCAAGCAGA CAGAGACTGG AAGAAAGTTG CAAAACAGGA AGACTTAAAA 
GAAATGGGAG ATGTTTCCTC GGGGATGAGT AGCTCCATCA TGCAGCTTTA CCTCAAACAG 
GTGCTTGAGG CCTTCTTCCA CACCCAGTCA AGTGTACGCC ACTTTGCCCT AAATGTGATT 
GCATTGACTC TAAACCAAGG TCTTATTCAT CCAGTGCAGT GTGTTCCATA TTTAATTGCC 
ATGGGCACAG ATCCAGAACC TGCTATGAGG AACAAGGCTG ATCAGCAGCT TGTGGAAATA 
GACAAAAAAT ATGCTGGATT CATTCATATG AAAGCAGTGG CTGGTATGAA GATGTCTTAC 
CAGGTACAGC AGGCGATCAA CACGTGCCTA AAAGACCCTG TCAGGGGTTT CAGACAAGAC 
GAGTCCTCTA GTGCTTTGTG CTCACACCTC TACACCATGA TCCGCGGGAA CCGCCAGCAC 
AGACGCGCCT TTCTCATCTC CTTACTCAAC CTCTTTGATG ACACAGCAAA AACAGAAGTG 
ACTATGCTCT TGTACATAGC AGACAATCTA GCCTGTTTTC CATACCAGAC TCAGGAAGAG 
CCGTTGTTTA TAATGCATCA CATAGACATT ACACTCTCTG TTTCCGGTAG TAATCTATTG 
CAGTCATTCA AGGAGTCTAT GGTAAAGGAC AAACGGAAAG AAAGAAAAAC ATCACCTGCT 
AAGGAGAACG AGTCCAGTGA GAGTGAAGAG GAAGTTTCCA GGCCTCGGAA GTCTCGGAAG 
CGGGTGGATT CAGATTCCGA CTCAGACTCC GAAGACGATG TAAACTCAGT GATGAAATGT 
CTACCAGAAA ACTCAGCCCC TTTAATTGAA TTTGCAAATG TTTCCCAGGG TATTTTGTTA 
CTCCTTATGT TAAAACAACA TTTGAAGAAT CTCTGTGGAT TTTCTGATAG TAAAATTCAG 
AAATATTCTC CATCTGAATC TGCAAAAGTA TATGATAAAG CCATTAACCG AAAAACAGGG 
GTTCATTTTC ATCCCAAACA AACACTGGAC TTCCTGCGAA GTGACATGGC TAATTCCAAG 
CTAACGGAGG ATGTGAAGAG GAGCATAGTA AGGCAGTACC TAGACTTTAA ACTTCTCATG 
GAACATCTGG ACCCTGATGA AGAAGAAGAG GAAGGCGAGG TGTCAGCCAG CACGAATGCT 
CGGAATAAAG CAATTACCTC GCTGCTTGGA GGAGGCAGCC CTAAAAATAA CACAGCAGCA 
GAGACAGAAG ATGATGAAAG TGATGGGGAG GATAGAGGAG GAGGCACTTC AGGGGTGAGG 
CGGAGGAGGA GTCAACGTAT TTCCCAGCGT ATTACTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NIPBL cDNA ORF clone, Mesocricetus auratus(Golden hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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