Arfgef3 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgef3
Entrez Gene ID	101835384
Full Name	ARFGEF family member 3
Synonyms	Kiaa1244
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013110177.1	XP_012965631.1	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
XM_013110177.2	XP_012965631.1	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
XM_005065842.2	XP_005065899.1	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
XM_005065842.3	XP_005065899.1	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following Arfgef3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe118812	XM_013110177.2 Latest version!	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe118813	XM_005065842.3 Latest version!	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMe118812	XM_013110177.1	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe118813	XM_005065842.2	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgef3 ( XM_013110177.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012965631.1 Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.

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CloneID	OMe118812
Clone ID Related Accession (Same CDS sequence)	XM_013110177.1 , XM_013110177.2
Accession Version	XM_013110177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801608.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGCAGAT 
ATTGATGGCT TAGAGAGCAG CGCAATTGGT GGCCAGTTGA TGGCATCAGC TTCAATGGAG 
TCACCTTTCA CCCAGAGCAG GAGAATTGAT GACTCCACTG TAGCAGGTGT GGCATTTGCT 
CGTTATATTT TGGTGGGCTG CTGGAAGAAC CTGATTGATA CTCTGTCAAC CCCCTTGACT 
GGTCGAATGG CAGGAAGCTC CAAGGGGCTG GCCTTCATCC TCGGAGCTGA GGGCATAAAA 
GAGCAGAACC AGAAGGAGCG AGATGCCATC TGCATGAGCC TTGACGGGCT GCGCAAAGCT 
GCACGACTGA GCTGTGCACT AGGGGTTGCT GCTAACTGCG CTTCAGCACT TGCCCAGATG 
GCGGCTGCTT CCTGTGTCCA GGAAGAGAAA GAAGAAAGGG AAGCCCAGGA GCCCAGTGAT 
GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAACTGG AGCAGATGGG GAAGGTGCAG 
GGGGTGTGGC TGCACACAGC CCACGTCTTG TGCATGGATG CCATCCTCAG CGTAGGCCTG 
GAGATGGGAA GCCACAACCC GGACTGCTGG CCACACGTGT TCAGGGTGTG TGAGTATGTG 
GGCACATTGG AGCACACTCA CTTCAGTGAT GGTGCCTCCC AGCCCCCTCT GACCATCCGT 
CAGCCACAGA AGACATCTGG GAGCTCTGGC CTCCTGGGGG ACCTTGAGTG TGAGGGTTCA 
TCTCAGGAGC AGAGCCTGGA GCAGGGCCCC TCCCTGAACA CAGCCCCTGT GGTCCAGCCA 
CGCTCCATCC AGGAGCTCGT TCGAGAATGT AGCCGTGGTA GGACCTCAGA CTTCAGGGGA 
GGCAGTCTGA GTGGGAACAG TGCCGCCAAG GTGGTACTCA GCCTTTCCAC CCAGGCTGAC 
AGGCTCTTTG ACGATGCTAC AGATAAGTTG AACCTAACGG CCTTGGGAGG GTTCCTCTAC 
CAGCTAAAGA AAGCGTCTCA GGCCCAGCTT TTCCACTCGG TGACAGACAC TGTCGATTAC 
TCTCTGGCAA TGCCAGGAGA GGTTAAGTCC ACCCAGGACC GCAAAAGCGC TCTGCACCTG 
TTCCGCCTGG GGGATGCCAT GCTGAGGATC GTGAGAAGCA AAGCCCGACC CCTGCTCCAC 
GTGATGCGCT GCTGGAGCCT TGTGGCACCA CACCTGGTGG AGGCGGCCTG CCACAAGGAG 
CGCCACGTGT CTCAGAAGGC TGTTTCCTTC ATCCATGATA TCCTGACCGA GGTTCTCACT 
GACTGGAGTG AGCCGCCTCA CTTCCACTTT AATGAAGCGC TCTTCCGGCC CTTTGAGCGT 
ATCATGCAGT TGGAGCTGTG TGACGAAGAT GTCCAAGACC AGGTGGTCAC CTCCATTGGG 
GAGCTGGTGG AGGTGTGCTC CACGCAGATC CAGTCGGGAT GGAGACCCCT GTTCAGTGCA 
CTGGAAACAG TGCACAGTGG GAACAAGTCG GAGGTGAAGG AGTACCTGGT TGGGGACTAC 
GCCATGGGAA AAGGCCAGGC TCCTGTGTTC GACGTGTTTG AAGCCTTTCT CAACACGGAC 
AACATCCAGG TCTTTGCCAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC 
AAAGGACTGG GGGAGGTGGA CTGTAAAGAG ATTGGAGACT GTGTCCCTGG AGTGGGAGCC 
ACATCCACAG ACCTGTGCCT GCCTGCTCTG GATTACCTCA GACGCTGCTC TCAGCTTTTG 
GCCAAGATCT ACAAAATGCC CTTAAAGCCA ATATTCCTCA GCGGGCGACT TGCCAGCTTG 
CCACGAAGGC TTCAGGAGCA GTCAGCCAGC AGCGAGGATG GAATTGAATC GGTCCTATCA 
GATTTTGATG ATGAAACTGG TCTGATAGAA GTTTGGATCA TCCTGCTGGA GCAGCTAACA 
GCAGCTGTGT CCAACTGTCC CCGGCAACAC CAACCACCAA CCTTAGATTT ACTCTTTGAG 
CTGTTGAGAG ATGTCACCAA AACACCTGGC CCAGGGTTTG GAATCTACGC AGTCGTTCAC 
CTTCTCCTTC CTGTGATGTC TCTGTGGCTC CGTCGTAGCC ACAAGGACCA TTCCTACTGG 
GATGTGGCAT CTGCGAACTT CAAGCATGCC ATCGGCCTGT CCTGCGAGCT GGTGGTGGAG 
CACATTCAGA GCTTCCTACA CTCAGACATC AGGTACGAGA GCATGATCAA CACCATGCTG 
AAGGATCTCT TTGAGCTGCT GGTGGCATGT GTGGCCAAAC CCACAGAAAC CATCTCCAGA 
GTGGGCTGTT CCTGCATTAG GTATGTCCTC GTGACTGCGG GTCCTGTGTT CACTGAGGAA 
ATGTGGCGAC TTGCCTGCTG TGCCCTGCAG GATGCCTTCT CTGCCACACT CAAGCCAGTG 
AAGGACCTGC TCGGCTGCTT CCACAGTGGC ACAGAGGGCT TCAGCGGAGA AGGCTGCCAG 
GTGCGGGTAG CAGCCCCATC CTCCTCCCCG AGTGCTGAGG CAGAGTACTG GCGCATCCGG 
GCCATGGCCC AGCAGGTGTT TATGTTGGAC ACCCAGGGCT CACCAAAGAC CCCAAACAAC 
TTCGATCATG CTCAGTCCTG CCAGCTCATC ATTGAACTGC CTCCTGATGA GAAACCAAAT 
GGGCACGCCA AGAAAAGTGT TTCTTTCAGG GAGATCGTGG TGAGCTTGCT TTCTCACCAA 
GTACTGCTCC AGAACCTGTA TGACGTCCTG CTGGAAGAGT TTGTCAAAGG CCCCTCTCCA 
GGAGAGGAGA AGACGACGGT GCAAGTGCCA GACACCAAGC TGGCTGGCTT TCTCAGATAC 
ATCTCCATGC AGAACCTGGC TGTGATATTT GACCTGCTGC TGGACTCCTA CAGGACTGCC 
CGAGAATTCG ACACGAGCCC AGGACTGAAG TGTCTGCTGA AGAAAGTGTC CGGCATCGGA 
GGCGCCGCCA ACCTGTACCG CCAGTCTGCC ATGAGCTTTA ACATTTACTT TCACGCCCTG 
GTTTGCGCAG TTCTCACCAA CCAAGAAACC ATCACTGCCG AGCAGGTGAA GAAGGTCCTC 
TTCGACGAGG AGGAGAGGAG CTCCGATTCC TCCCAACAGT GCTCATCGGA GGATGAAGAC 
ATCTTTGAGG AGACGGCGCA GGTGAGCCCT CCGCGAGGCA AGGAGAAAAG GCAATGGAGG 
GCACGCCTGC CGTCGCTCAG CGTGCAGCCG GTGAGCAATG CAGACTGGGT GTGGCTGGTC 
AAGAGGCTAC ACAAGCTGTG CATGGAGCTC TGTAACCACT ACATCCAGAT GCACCTGGAC 
CTGGAGAGCA GCTTAGAGGA GCCTCCCGCC TTCAAAAGCG ACCCGTTCTT CATCCTGCCA 
TCCTTCCAGT CTGAGTCGTC CACACCATCC ACGGGTGGCT TCTCCGGGAA AAACACGCCC 
TCGGAAGATG ACCGCAGAGA GCATCTAAGT GAGCCCCTAA GTCTGAGGGT GGGCAGTGGG 
GACGTGCTGA TGCTGCCCCC CAGTCCCAAG ACAGAGAAGA AGGATCCTGG CCGCAAGAAA 
GAGTGGTGGG AGAGCGCGGG GAACAAGATT TGCACCATGG CTGCCGACAA AACCATCTCA 
AAGCTGATGA CCGAGTACAA GAAGAGGAGG CAGCCGCACA ACCTGCCTGC CTTCCCCAAG 
GAAGTCAAGG CAGATAAGAA GGGCGAGCCC CTGGGGCCTC GGGGTCCTGA TTCCCCGCTG 
CTTCAGCGGC CACAGCATCT CATGGACCAA GGACAGATGC GCCATTCCTT CAGTGCAGGC 
CCAGAGCTGT TGCGGCAGGA GAAGAGACCC CGCTCAGGCT CCACCGGGAG CTCCCTGAGT 
GTCTCGGTGC GAGACGCAGA GGCACAGATC CAGGCGTGGA CCAACATGGT GCTAACAGTT 
CTCAATCAGA TTCAAATCCT TCCAGACCAG ACATTCACGG CCCTCCAGCC AGCAGTGTTC 
CCGTGCATCA GCCAGCTGAC CTGTCATGTG ACTGACATCA GAGTCCGTCA AGCTGTAAGG 
GAATGGCTGG GAAGAGTGGG CCGTGTCTAT GATATCATCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012965631.1
CDS	86..6610
Translation	MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTIVKIPPHLLREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSRETDSDEKQLLNQILNAVKVTPLLNEDLQVEVMKVLLCITYTSTFDMNGSAVLKIAEVCIQTYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFGSQGSTVEALCDDVVSVLAVLCEKLQASINDSQQLQLLYLECILSMLSSSSSCMYLHRGFTDLIWKSLCPALIVILGNPIHDKTITSAHSSSSSSSTNVESDSASPGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSTELEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLNESQVQLLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEHTPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHRNGFKSPAIQEGKETMCKVSEQEAIDQPDVVQRSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDNCSLADEEQTPRDYMGHRSLRTAALSLKLLKNQEADQHSARLFVQSLEGLLPRLLALSSVEEVDTALQNFASTFCSGMMHSPGFDGGSSLSFQMLMNADSLYSAAHCALLLNLKLSHGDYYRKRPTLAPGMMKEFMKQVQTSGVLMVFSQAWIEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTADIDGLESSAIGGQLMASASMESPFTQSRRIDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEEREAQEPSDAIAQVKLKVEQKLEQMGKVQGVWLHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGASQPPLTIRQPQKTSGSSGLLGDLECEGSSQEQSLEQGPSLNTAPVVQPRSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLFDDATDKLNLTALGGFLYQLKKASQAQLFHSVTDTVDYSLAMPGEVKSTQDRKSALHLFRLGDAMLRIVRSKARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQLELCDEDVQDQVVTSIGELVEVCSTQIQSGWRPLFSALETVHSGNKSEVKEYLVGDYAMGKGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGVGATSTDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDETGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLRRSHKDHSYWDVASANFKHAIGLSCELVVEHIQSFLHSDIRYESMINTMLKDLFELLVACVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAFSATLKPVKDLLGCFHSGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQGSPKTPNNFDHAQSCQLIIELPPDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDVLLEEFVKGPSPGEEKTTVQVPDTKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFDEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNADWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPPAFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDDRREHLSEPLSLRVGSGDVLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHNLPAFPKEVKADKKGEPLGPRGPDSPLLQRPQHLMDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIII

Target ORF information:

RefSeq Version	XM_013110177.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013110177.1

ORF Insert Sequence:

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGCAGAT 
ATTGATGGCT TAGAGAGCAG CGCAATTGGT GGCCAGTTGA TGGCATCAGC TTCAATGGAG 
TCACCTTTCA CCCAGAGCAG GAGAATTGAT GACTCCACTG TAGCAGGTGT GGCATTTGCT 
CGTTATATTT TGGTGGGCTG CTGGAAGAAC CTGATTGATA CTCTGTCAAC CCCCTTGACT 
GGTCGAATGG CAGGAAGCTC CAAGGGGCTG GCCTTCATCC TCGGAGCTGA GGGCATAAAA 
GAGCAGAACC AGAAGGAGCG AGATGCCATC TGCATGAGCC TTGACGGGCT GCGCAAAGCT 
GCACGACTGA GCTGTGCACT AGGGGTTGCT GCTAACTGCG CTTCAGCACT TGCCCAGATG 
GCGGCTGCTT CCTGTGTCCA GGAAGAGAAA GAAGAAAGGG AAGCCCAGGA GCCCAGTGAT 
GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAACTGG AGCAGATGGG GAAGGTGCAG 
GGGGTGTGGC TGCACACAGC CCACGTCTTG TGCATGGATG CCATCCTCAG CGTAGGCCTG 
GAGATGGGAA GCCACAACCC GGACTGCTGG CCACACGTGT TCAGGGTGTG TGAGTATGTG 
GGCACATTGG AGCACACTCA CTTCAGTGAT GGTGCCTCCC AGCCCCCTCT GACCATCCGT 
CAGCCACAGA AGACATCTGG GAGCTCTGGC CTCCTGGGGG ACCTTGAGTG TGAGGGTTCA 
TCTCAGGAGC AGAGCCTGGA GCAGGGCCCC TCCCTGAACA CAGCCCCTGT GGTCCAGCCA 
CGCTCCATCC AGGAGCTCGT TCGAGAATGT AGCCGTGGTA GGACCTCAGA CTTCAGGGGA 
GGCAGTCTGA GTGGGAACAG TGCCGCCAAG GTGGTACTCA GCCTTTCCAC CCAGGCTGAC 
AGGCTCTTTG ACGATGCTAC AGATAAGTTG AACCTAACGG CCTTGGGAGG GTTCCTCTAC 
CAGCTAAAGA AAGCGTCTCA GGCCCAGCTT TTCCACTCGG TGACAGACAC TGTCGATTAC 
TCTCTGGCAA TGCCAGGAGA GGTTAAGTCC ACCCAGGACC GCAAAAGCGC TCTGCACCTG 
TTCCGCCTGG GGGATGCCAT GCTGAGGATC GTGAGAAGCA AAGCCCGACC CCTGCTCCAC 
GTGATGCGCT GCTGGAGCCT TGTGGCACCA CACCTGGTGG AGGCGGCCTG CCACAAGGAG 
CGCCACGTGT CTCAGAAGGC TGTTTCCTTC ATCCATGATA TCCTGACCGA GGTTCTCACT 
GACTGGAGTG AGCCGCCTCA CTTCCACTTT AATGAAGCGC TCTTCCGGCC CTTTGAGCGT 
ATCATGCAGT TGGAGCTGTG TGACGAAGAT GTCCAAGACC AGGTGGTCAC CTCCATTGGG 
GAGCTGGTGG AGGTGTGCTC CACGCAGATC CAGTCGGGAT GGAGACCCCT GTTCAGTGCA 
CTGGAAACAG TGCACAGTGG GAACAAGTCG GAGGTGAAGG AGTACCTGGT TGGGGACTAC 
GCCATGGGAA AAGGCCAGGC TCCTGTGTTC GACGTGTTTG AAGCCTTTCT CAACACGGAC 
AACATCCAGG TCTTTGCCAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC 
AAAGGACTGG GGGAGGTGGA CTGTAAAGAG ATTGGAGACT GTGTCCCTGG AGTGGGAGCC 
ACATCCACAG ACCTGTGCCT GCCTGCTCTG GATTACCTCA GACGCTGCTC TCAGCTTTTG 
GCCAAGATCT ACAAAATGCC CTTAAAGCCA ATATTCCTCA GCGGGCGACT TGCCAGCTTG 
CCACGAAGGC TTCAGGAGCA GTCAGCCAGC AGCGAGGATG GAATTGAATC GGTCCTATCA 
GATTTTGATG ATGAAACTGG TCTGATAGAA GTTTGGATCA TCCTGCTGGA GCAGCTAACA 
GCAGCTGTGT CCAACTGTCC CCGGCAACAC CAACCACCAA CCTTAGATTT ACTCTTTGAG 
CTGTTGAGAG ATGTCACCAA AACACCTGGC CCAGGGTTTG GAATCTACGC AGTCGTTCAC 
CTTCTCCTTC CTGTGATGTC TCTGTGGCTC CGTCGTAGCC ACAAGGACCA TTCCTACTGG 
GATGTGGCAT CTGCGAACTT CAAGCATGCC ATCGGCCTGT CCTGCGAGCT GGTGGTGGAG 
CACATTCAGA GCTTCCTACA CTCAGACATC AGGTACGAGA GCATGATCAA CACCATGCTG 
AAGGATCTCT TTGAGCTGCT GGTGGCATGT GTGGCCAAAC CCACAGAAAC CATCTCCAGA 
GTGGGCTGTT CCTGCATTAG GTATGTCCTC GTGACTGCGG GTCCTGTGTT CACTGAGGAA 
ATGTGGCGAC TTGCCTGCTG TGCCCTGCAG GATGCCTTCT CTGCCACACT CAAGCCAGTG 
AAGGACCTGC TCGGCTGCTT CCACAGTGGC ACAGAGGGCT TCAGCGGAGA AGGCTGCCAG 
GTGCGGGTAG CAGCCCCATC CTCCTCCCCG AGTGCTGAGG CAGAGTACTG GCGCATCCGG 
GCCATGGCCC AGCAGGTGTT TATGTTGGAC ACCCAGGGCT CACCAAAGAC CCCAAACAAC 
TTCGATCATG CTCAGTCCTG CCAGCTCATC ATTGAACTGC CTCCTGATGA GAAACCAAAT 
GGGCACGCCA AGAAAAGTGT TTCTTTCAGG GAGATCGTGG TGAGCTTGCT TTCTCACCAA 
GTACTGCTCC AGAACCTGTA TGACGTCCTG CTGGAAGAGT TTGTCAAAGG CCCCTCTCCA 
GGAGAGGAGA AGACGACGGT GCAAGTGCCA GACACCAAGC TGGCTGGCTT TCTCAGATAC 
ATCTCCATGC AGAACCTGGC TGTGATATTT GACCTGCTGC TGGACTCCTA CAGGACTGCC 
CGAGAATTCG ACACGAGCCC AGGACTGAAG TGTCTGCTGA AGAAAGTGTC CGGCATCGGA 
GGCGCCGCCA ACCTGTACCG CCAGTCTGCC ATGAGCTTTA ACATTTACTT TCACGCCCTG 
GTTTGCGCAG TTCTCACCAA CCAAGAAACC ATCACTGCCG AGCAGGTGAA GAAGGTCCTC 
TTCGACGAGG AGGAGAGGAG CTCCGATTCC TCCCAACAGT GCTCATCGGA GGATGAAGAC 
ATCTTTGAGG AGACGGCGCA GGTGAGCCCT CCGCGAGGCA AGGAGAAAAG GCAATGGAGG 
GCACGCCTGC CGTCGCTCAG CGTGCAGCCG GTGAGCAATG CAGACTGGGT GTGGCTGGTC 
AAGAGGCTAC ACAAGCTGTG CATGGAGCTC TGTAACCACT ACATCCAGAT GCACCTGGAC 
CTGGAGAGCA GCTTAGAGGA GCCTCCCGCC TTCAAAAGCG ACCCGTTCTT CATCCTGCCA 
TCCTTCCAGT CTGAGTCGTC CACACCATCC ACGGGTGGCT TCTCCGGGAA AAACACGCCC 
TCGGAAGATG ACCGCAGAGA GCATCTAAGT GAGCCCCTAA GTCTGAGGGT GGGCAGTGGG 
GACGTGCTGA TGCTGCCCCC CAGTCCCAAG ACAGAGAAGA AGGATCCTGG CCGCAAGAAA 
GAGTGGTGGG AGAGCGCGGG GAACAAGATT TGCACCATGG CTGCCGACAA AACCATCTCA 
AAGCTGATGA CCGAGTACAA GAAGAGGAGG CAGCCGCACA ACCTGCCTGC CTTCCCCAAG 
GAAGTCAAGG CAGATAAGAA GGGCGAGCCC CTGGGGCCTC GGGGTCCTGA TTCCCCGCTG 
CTTCAGCGGC CACAGCATCT CATGGACCAA GGACAGATGC GCCATTCCTT CAGTGCAGGC 
CCAGAGCTGT TGCGGCAGGA GAAGAGACCC CGCTCAGGCT CCACCGGGAG CTCCCTGAGT 
GTCTCGGTGC GAGACGCAGA GGCACAGATC CAGGCGTGGA CCAACATGGT GCTAACAGTT 
CTCAATCAGA TTCAAATCCT TCCAGACCAG ACATTCACGG CCCTCCAGCC AGCAGTGTTC 
CCGTGCATCA GCCAGCTGAC CTGTCATGTG ACTGACATCA GAGTCCGTCA AGCTGTAAGG 
GAATGGCTGG GAAGAGTGGG CCGTGTCTAT GATATCATCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef3 ( XM_013110177.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012965631.1 Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.

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CloneID	OMe118812
Clone ID Related Accession (Same CDS sequence)	XM_013110177.1 , XM_013110177.2
Accession Version	XM_013110177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801608.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013110177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGCAGAT 
ATTGATGGCT TAGAGAGCAG CGCAATTGGT GGCCAGTTGA TGGCATCAGC TTCAATGGAG 
TCACCTTTCA CCCAGAGCAG GAGAATTGAT GACTCCACTG TAGCAGGTGT GGCATTTGCT 
CGTTATATTT TGGTGGGCTG CTGGAAGAAC CTGATTGATA CTCTGTCAAC CCCCTTGACT 
GGTCGAATGG CAGGAAGCTC CAAGGGGCTG GCCTTCATCC TCGGAGCTGA GGGCATAAAA 
GAGCAGAACC AGAAGGAGCG AGATGCCATC TGCATGAGCC TTGACGGGCT GCGCAAAGCT 
GCACGACTGA GCTGTGCACT AGGGGTTGCT GCTAACTGCG CTTCAGCACT TGCCCAGATG 
GCGGCTGCTT CCTGTGTCCA GGAAGAGAAA GAAGAAAGGG AAGCCCAGGA GCCCAGTGAT 
GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAACTGG AGCAGATGGG GAAGGTGCAG 
GGGGTGTGGC TGCACACAGC CCACGTCTTG TGCATGGATG CCATCCTCAG CGTAGGCCTG 
GAGATGGGAA GCCACAACCC GGACTGCTGG CCACACGTGT TCAGGGTGTG TGAGTATGTG 
GGCACATTGG AGCACACTCA CTTCAGTGAT GGTGCCTCCC AGCCCCCTCT GACCATCCGT 
CAGCCACAGA AGACATCTGG GAGCTCTGGC CTCCTGGGGG ACCTTGAGTG TGAGGGTTCA 
TCTCAGGAGC AGAGCCTGGA GCAGGGCCCC TCCCTGAACA CAGCCCCTGT GGTCCAGCCA 
CGCTCCATCC AGGAGCTCGT TCGAGAATGT AGCCGTGGTA GGACCTCAGA CTTCAGGGGA 
GGCAGTCTGA GTGGGAACAG TGCCGCCAAG GTGGTACTCA GCCTTTCCAC CCAGGCTGAC 
AGGCTCTTTG ACGATGCTAC AGATAAGTTG AACCTAACGG CCTTGGGAGG GTTCCTCTAC 
CAGCTAAAGA AAGCGTCTCA GGCCCAGCTT TTCCACTCGG TGACAGACAC TGTCGATTAC 
TCTCTGGCAA TGCCAGGAGA GGTTAAGTCC ACCCAGGACC GCAAAAGCGC TCTGCACCTG 
TTCCGCCTGG GGGATGCCAT GCTGAGGATC GTGAGAAGCA AAGCCCGACC CCTGCTCCAC 
GTGATGCGCT GCTGGAGCCT TGTGGCACCA CACCTGGTGG AGGCGGCCTG CCACAAGGAG 
CGCCACGTGT CTCAGAAGGC TGTTTCCTTC ATCCATGATA TCCTGACCGA GGTTCTCACT 
GACTGGAGTG AGCCGCCTCA CTTCCACTTT AATGAAGCGC TCTTCCGGCC CTTTGAGCGT 
ATCATGCAGT TGGAGCTGTG TGACGAAGAT GTCCAAGACC AGGTGGTCAC CTCCATTGGG 
GAGCTGGTGG AGGTGTGCTC CACGCAGATC CAGTCGGGAT GGAGACCCCT GTTCAGTGCA 
CTGGAAACAG TGCACAGTGG GAACAAGTCG GAGGTGAAGG AGTACCTGGT TGGGGACTAC 
GCCATGGGAA AAGGCCAGGC TCCTGTGTTC GACGTGTTTG AAGCCTTTCT CAACACGGAC 
AACATCCAGG TCTTTGCCAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC 
AAAGGACTGG GGGAGGTGGA CTGTAAAGAG ATTGGAGACT GTGTCCCTGG AGTGGGAGCC 
ACATCCACAG ACCTGTGCCT GCCTGCTCTG GATTACCTCA GACGCTGCTC TCAGCTTTTG 
GCCAAGATCT ACAAAATGCC CTTAAAGCCA ATATTCCTCA GCGGGCGACT TGCCAGCTTG 
CCACGAAGGC TTCAGGAGCA GTCAGCCAGC AGCGAGGATG GAATTGAATC GGTCCTATCA 
GATTTTGATG ATGAAACTGG TCTGATAGAA GTTTGGATCA TCCTGCTGGA GCAGCTAACA 
GCAGCTGTGT CCAACTGTCC CCGGCAACAC CAACCACCAA CCTTAGATTT ACTCTTTGAG 
CTGTTGAGAG ATGTCACCAA AACACCTGGC CCAGGGTTTG GAATCTACGC AGTCGTTCAC 
CTTCTCCTTC CTGTGATGTC TCTGTGGCTC CGTCGTAGCC ACAAGGACCA TTCCTACTGG 
GATGTGGCAT CTGCGAACTT CAAGCATGCC ATCGGCCTGT CCTGCGAGCT GGTGGTGGAG 
CACATTCAGA GCTTCCTACA CTCAGACATC AGGTACGAGA GCATGATCAA CACCATGCTG 
AAGGATCTCT TTGAGCTGCT GGTGGCATGT GTGGCCAAAC CCACAGAAAC CATCTCCAGA 
GTGGGCTGTT CCTGCATTAG GTATGTCCTC GTGACTGCGG GTCCTGTGTT CACTGAGGAA 
ATGTGGCGAC TTGCCTGCTG TGCCCTGCAG GATGCCTTCT CTGCCACACT CAAGCCAGTG 
AAGGACCTGC TCGGCTGCTT CCACAGTGGC ACAGAGGGCT TCAGCGGAGA AGGCTGCCAG 
GTGCGGGTAG CAGCCCCATC CTCCTCCCCG AGTGCTGAGG CAGAGTACTG GCGCATCCGG 
GCCATGGCCC AGCAGGTGTT TATGTTGGAC ACCCAGGGCT CACCAAAGAC CCCAAACAAC 
TTCGATCATG CTCAGTCCTG CCAGCTCATC ATTGAACTGC CTCCTGATGA GAAACCAAAT 
GGGCACGCCA AGAAAAGTGT TTCTTTCAGG GAGATCGTGG TGAGCTTGCT TTCTCACCAA 
GTACTGCTCC AGAACCTGTA TGACGTCCTG CTGGAAGAGT TTGTCAAAGG CCCCTCTCCA 
GGAGAGGAGA AGACGACGGT GCAAGTGCCA GACACCAAGC TGGCTGGCTT TCTCAGATAC 
ATCTCCATGC AGAACCTGGC TGTGATATTT GACCTGCTGC TGGACTCCTA CAGGACTGCC 
CGAGAATTCG ACACGAGCCC AGGACTGAAG TGTCTGCTGA AGAAAGTGTC CGGCATCGGA 
GGCGCCGCCA ACCTGTACCG CCAGTCTGCC ATGAGCTTTA ACATTTACTT TCACGCCCTG 
GTTTGCGCAG TTCTCACCAA CCAAGAAACC ATCACTGCCG AGCAGGTGAA GAAGGTCCTC 
TTCGACGAGG AGGAGAGGAG CTCCGATTCC TCCCAACAGT GCTCATCGGA GGATGAAGAC 
ATCTTTGAGG AGACGGCGCA GGTGAGCCCT CCGCGAGGCA AGGAGAAAAG GCAATGGAGG 
GCACGCCTGC CGTCGCTCAG CGTGCAGCCG GTGAGCAATG CAGACTGGGT GTGGCTGGTC 
AAGAGGCTAC ACAAGCTGTG CATGGAGCTC TGTAACCACT ACATCCAGAT GCACCTGGAC 
CTGGAGAGCA GCTTAGAGGA GCCTCCCGCC TTCAAAAGCG ACCCGTTCTT CATCCTGCCA 
TCCTTCCAGT CTGAGTCGTC CACACCATCC ACGGGTGGCT TCTCCGGGAA AAACACGCCC 
TCGGAAGATG ACCGCAGAGA GCATCTAAGT GAGCCCCTAA GTCTGAGGGT GGGCAGTGGG 
GACGTGCTGA TGCTGCCCCC CAGTCCCAAG ACAGAGAAGA AGGATCCTGG CCGCAAGAAA 
GAGTGGTGGG AGAGCGCGGG GAACAAGATT TGCACCATGG CTGCCGACAA AACCATCTCA 
AAGCTGATGA CCGAGTACAA GAAGAGGAGG CAGCCGCACA ACCTGCCTGC CTTCCCCAAG 
GAAGTCAAGG CAGATAAGAA GGGCGAGCCC CTGGGGCCTC GGGGTCCTGA TTCCCCGCTG 
CTTCAGCGGC CACAGCATCT CATGGACCAA GGACAGATGC GCCATTCCTT CAGTGCAGGC 
CCAGAGCTGT TGCGGCAGGA GAAGAGACCC CGCTCAGGCT CCACCGGGAG CTCCCTGAGT 
GTCTCGGTGC GAGACGCAGA GGCACAGATC CAGGCGTGGA CCAACATGGT GCTAACAGTT 
CTCAATCAGA TTCAAATCCT TCCAGACCAG ACATTCACGG CCCTCCAGCC AGCAGTGTTC 
CCGTGCATCA GCCAGCTGAC CTGTCATGTG ACTGACATCA GAGTCCGTCA AGCTGTAAGG 
GAATGGCTGG GAAGAGTGGG CCGTGTCTAT GATATCATCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012965631.1
CDS	102..6626
Translation	MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTIVKIPPHLLREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSRETDSDEKQLLNQILNAVKVTPLLNEDLQVEVMKVLLCITYTSTFDMNGSAVLKIAEVCIQTYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFGSQGSTVEALCDDVVSVLAVLCEKLQASINDSQQLQLLYLECILSMLSSSSSCMYLHRGFTDLIWKSLCPALIVILGNPIHDKTITSAHSSSSSSSTNVESDSASPGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSTELEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLNESQVQLLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEHTPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHRNGFKSPAIQEGKETMCKVSEQEAIDQPDVVQRSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDNCSLADEEQTPRDYMGHRSLRTAALSLKLLKNQEADQHSARLFVQSLEGLLPRLLALSSVEEVDTALQNFASTFCSGMMHSPGFDGGSSLSFQMLMNADSLYSAAHCALLLNLKLSHGDYYRKRPTLAPGMMKEFMKQVQTSGVLMVFSQAWIEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTADIDGLESSAIGGQLMASASMESPFTQSRRIDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEEREAQEPSDAIAQVKLKVEQKLEQMGKVQGVWLHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGASQPPLTIRQPQKTSGSSGLLGDLECEGSSQEQSLEQGPSLNTAPVVQPRSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLFDDATDKLNLTALGGFLYQLKKASQAQLFHSVTDTVDYSLAMPGEVKSTQDRKSALHLFRLGDAMLRIVRSKARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQLELCDEDVQDQVVTSIGELVEVCSTQIQSGWRPLFSALETVHSGNKSEVKEYLVGDYAMGKGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGVGATSTDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDETGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLRRSHKDHSYWDVASANFKHAIGLSCELVVEHIQSFLHSDIRYESMINTMLKDLFELLVACVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAFSATLKPVKDLLGCFHSGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQGSPKTPNNFDHAQSCQLIIELPPDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDVLLEEFVKGPSPGEEKTTVQVPDTKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFDEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNADWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPPAFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDDRREHLSEPLSLRVGSGDVLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHNLPAFPKEVKADKKGEPLGPRGPDSPLLQRPQHLMDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIII

Target ORF information:

RefSeq Version	XM_013110177.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013110177.2

ORF Insert Sequence:

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGCAGAT 
ATTGATGGCT TAGAGAGCAG CGCAATTGGT GGCCAGTTGA TGGCATCAGC TTCAATGGAG 
TCACCTTTCA CCCAGAGCAG GAGAATTGAT GACTCCACTG TAGCAGGTGT GGCATTTGCT 
CGTTATATTT TGGTGGGCTG CTGGAAGAAC CTGATTGATA CTCTGTCAAC CCCCTTGACT 
GGTCGAATGG CAGGAAGCTC CAAGGGGCTG GCCTTCATCC TCGGAGCTGA GGGCATAAAA 
GAGCAGAACC AGAAGGAGCG AGATGCCATC TGCATGAGCC TTGACGGGCT GCGCAAAGCT 
GCACGACTGA GCTGTGCACT AGGGGTTGCT GCTAACTGCG CTTCAGCACT TGCCCAGATG 
GCGGCTGCTT CCTGTGTCCA GGAAGAGAAA GAAGAAAGGG AAGCCCAGGA GCCCAGTGAT 
GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAACTGG AGCAGATGGG GAAGGTGCAG 
GGGGTGTGGC TGCACACAGC CCACGTCTTG TGCATGGATG CCATCCTCAG CGTAGGCCTG 
GAGATGGGAA GCCACAACCC GGACTGCTGG CCACACGTGT TCAGGGTGTG TGAGTATGTG 
GGCACATTGG AGCACACTCA CTTCAGTGAT GGTGCCTCCC AGCCCCCTCT GACCATCCGT 
CAGCCACAGA AGACATCTGG GAGCTCTGGC CTCCTGGGGG ACCTTGAGTG TGAGGGTTCA 
TCTCAGGAGC AGAGCCTGGA GCAGGGCCCC TCCCTGAACA CAGCCCCTGT GGTCCAGCCA 
CGCTCCATCC AGGAGCTCGT TCGAGAATGT AGCCGTGGTA GGACCTCAGA CTTCAGGGGA 
GGCAGTCTGA GTGGGAACAG TGCCGCCAAG GTGGTACTCA GCCTTTCCAC CCAGGCTGAC 
AGGCTCTTTG ACGATGCTAC AGATAAGTTG AACCTAACGG CCTTGGGAGG GTTCCTCTAC 
CAGCTAAAGA AAGCGTCTCA GGCCCAGCTT TTCCACTCGG TGACAGACAC TGTCGATTAC 
TCTCTGGCAA TGCCAGGAGA GGTTAAGTCC ACCCAGGACC GCAAAAGCGC TCTGCACCTG 
TTCCGCCTGG GGGATGCCAT GCTGAGGATC GTGAGAAGCA AAGCCCGACC CCTGCTCCAC 
GTGATGCGCT GCTGGAGCCT TGTGGCACCA CACCTGGTGG AGGCGGCCTG CCACAAGGAG 
CGCCACGTGT CTCAGAAGGC TGTTTCCTTC ATCCATGATA TCCTGACCGA GGTTCTCACT 
GACTGGAGTG AGCCGCCTCA CTTCCACTTT AATGAAGCGC TCTTCCGGCC CTTTGAGCGT 
ATCATGCAGT TGGAGCTGTG TGACGAAGAT GTCCAAGACC AGGTGGTCAC CTCCATTGGG 
GAGCTGGTGG AGGTGTGCTC CACGCAGATC CAGTCGGGAT GGAGACCCCT GTTCAGTGCA 
CTGGAAACAG TGCACAGTGG GAACAAGTCG GAGGTGAAGG AGTACCTGGT TGGGGACTAC 
GCCATGGGAA AAGGCCAGGC TCCTGTGTTC GACGTGTTTG AAGCCTTTCT CAACACGGAC 
AACATCCAGG TCTTTGCCAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC 
AAAGGACTGG GGGAGGTGGA CTGTAAAGAG ATTGGAGACT GTGTCCCTGG AGTGGGAGCC 
ACATCCACAG ACCTGTGCCT GCCTGCTCTG GATTACCTCA GACGCTGCTC TCAGCTTTTG 
GCCAAGATCT ACAAAATGCC CTTAAAGCCA ATATTCCTCA GCGGGCGACT TGCCAGCTTG 
CCACGAAGGC TTCAGGAGCA GTCAGCCAGC AGCGAGGATG GAATTGAATC GGTCCTATCA 
GATTTTGATG ATGAAACTGG TCTGATAGAA GTTTGGATCA TCCTGCTGGA GCAGCTAACA 
GCAGCTGTGT CCAACTGTCC CCGGCAACAC CAACCACCAA CCTTAGATTT ACTCTTTGAG 
CTGTTGAGAG ATGTCACCAA AACACCTGGC CCAGGGTTTG GAATCTACGC AGTCGTTCAC 
CTTCTCCTTC CTGTGATGTC TCTGTGGCTC CGTCGTAGCC ACAAGGACCA TTCCTACTGG 
GATGTGGCAT CTGCGAACTT CAAGCATGCC ATCGGCCTGT CCTGCGAGCT GGTGGTGGAG 
CACATTCAGA GCTTCCTACA CTCAGACATC AGGTACGAGA GCATGATCAA CACCATGCTG 
AAGGATCTCT TTGAGCTGCT GGTGGCATGT GTGGCCAAAC CCACAGAAAC CATCTCCAGA 
GTGGGCTGTT CCTGCATTAG GTATGTCCTC GTGACTGCGG GTCCTGTGTT CACTGAGGAA 
ATGTGGCGAC TTGCCTGCTG TGCCCTGCAG GATGCCTTCT CTGCCACACT CAAGCCAGTG 
AAGGACCTGC TCGGCTGCTT CCACAGTGGC ACAGAGGGCT TCAGCGGAGA AGGCTGCCAG 
GTGCGGGTAG CAGCCCCATC CTCCTCCCCG AGTGCTGAGG CAGAGTACTG GCGCATCCGG 
GCCATGGCCC AGCAGGTGTT TATGTTGGAC ACCCAGGGCT CACCAAAGAC CCCAAACAAC 
TTCGATCATG CTCAGTCCTG CCAGCTCATC ATTGAACTGC CTCCTGATGA GAAACCAAAT 
GGGCACGCCA AGAAAAGTGT TTCTTTCAGG GAGATCGTGG TGAGCTTGCT TTCTCACCAA 
GTACTGCTCC AGAACCTGTA TGACGTCCTG CTGGAAGAGT TTGTCAAAGG CCCCTCTCCA 
GGAGAGGAGA AGACGACGGT GCAAGTGCCA GACACCAAGC TGGCTGGCTT TCTCAGATAC 
ATCTCCATGC AGAACCTGGC TGTGATATTT GACCTGCTGC TGGACTCCTA CAGGACTGCC 
CGAGAATTCG ACACGAGCCC AGGACTGAAG TGTCTGCTGA AGAAAGTGTC CGGCATCGGA 
GGCGCCGCCA ACCTGTACCG CCAGTCTGCC ATGAGCTTTA ACATTTACTT TCACGCCCTG 
GTTTGCGCAG TTCTCACCAA CCAAGAAACC ATCACTGCCG AGCAGGTGAA GAAGGTCCTC 
TTCGACGAGG AGGAGAGGAG CTCCGATTCC TCCCAACAGT GCTCATCGGA GGATGAAGAC 
ATCTTTGAGG AGACGGCGCA GGTGAGCCCT CCGCGAGGCA AGGAGAAAAG GCAATGGAGG 
GCACGCCTGC CGTCGCTCAG CGTGCAGCCG GTGAGCAATG CAGACTGGGT GTGGCTGGTC 
AAGAGGCTAC ACAAGCTGTG CATGGAGCTC TGTAACCACT ACATCCAGAT GCACCTGGAC 
CTGGAGAGCA GCTTAGAGGA GCCTCCCGCC TTCAAAAGCG ACCCGTTCTT CATCCTGCCA 
TCCTTCCAGT CTGAGTCGTC CACACCATCC ACGGGTGGCT TCTCCGGGAA AAACACGCCC 
TCGGAAGATG ACCGCAGAGA GCATCTAAGT GAGCCCCTAA GTCTGAGGGT GGGCAGTGGG 
GACGTGCTGA TGCTGCCCCC CAGTCCCAAG ACAGAGAAGA AGGATCCTGG CCGCAAGAAA 
GAGTGGTGGG AGAGCGCGGG GAACAAGATT TGCACCATGG CTGCCGACAA AACCATCTCA 
AAGCTGATGA CCGAGTACAA GAAGAGGAGG CAGCCGCACA ACCTGCCTGC CTTCCCCAAG 
GAAGTCAAGG CAGATAAGAA GGGCGAGCCC CTGGGGCCTC GGGGTCCTGA TTCCCCGCTG 
CTTCAGCGGC CACAGCATCT CATGGACCAA GGACAGATGC GCCATTCCTT CAGTGCAGGC 
CCAGAGCTGT TGCGGCAGGA GAAGAGACCC CGCTCAGGCT CCACCGGGAG CTCCCTGAGT 
GTCTCGGTGC GAGACGCAGA GGCACAGATC CAGGCGTGGA CCAACATGGT GCTAACAGTT 
CTCAATCAGA TTCAAATCCT TCCAGACCAG ACATTCACGG CCCTCCAGCC AGCAGTGTTC 
CCGTGCATCA GCCAGCTGAC CTGTCATGTG ACTGACATCA GAGTCCGTCA AGCTGTAAGG 
GAATGGCTGG GAAGAGTGGG CCGTGTCTAT GATATCATCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef3 ( XM_005065842.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005065899.1 Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.

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CloneID	OMe118813
Clone ID Related Accession (Same CDS sequence)	XM_005065842.2 , XM_005065842.3
Accession Version	XM_005065842.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801608.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005065842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGATATT 
GATGGCTTAG AGAGCAGCGC AATTGGTGGC CAGTTGATGG CATCAGCTTC AATGGAGTCA 
CCTTTCACCC AGAGCAGGAG AATTGATGAC TCCACTGTAG CAGGTGTGGC ATTTGCTCGT 
TATATTTTGG TGGGCTGCTG GAAGAACCTG ATTGATACTC TGTCAACCCC CTTGACTGGT 
CGAATGGCAG GAAGCTCCAA GGGGCTGGCC TTCATCCTCG GAGCTGAGGG CATAAAAGAG 
CAGAACCAGA AGGAGCGAGA TGCCATCTGC ATGAGCCTTG ACGGGCTGCG CAAAGCTGCA 
CGACTGAGCT GTGCACTAGG GGTTGCTGCT AACTGCGCTT CAGCACTTGC CCAGATGGCG 
GCTGCTTCCT GTGTCCAGGA AGAGAAAGAA GAAAGGGAAG CCCAGGAGCC CAGTGATGCC 
ATAGCACAAG TGAAACTAAA AGTGGAGCAG AAACTGGAGC AGATGGGGAA GGTGCAGGGG 
GTGTGGCTGC ACACAGCCCA CGTCTTGTGC ATGGATGCCA TCCTCAGCGT AGGCCTGGAG 
ATGGGAAGCC ACAACCCGGA CTGCTGGCCA CACGTGTTCA GGGTGTGTGA GTATGTGGGC 
ACATTGGAGC ACACTCACTT CAGTGATGGT GCCTCCCAGC CCCCTCTGAC CATCCGTCAG 
CCACAGAAGA CATCTGGGAG CTCTGGCCTC CTGGGGGACC TTGAGTGTGA GGGTTCATCT 
CAGGAGCAGA GCCTGGAGCA GGGCCCCTCC CTGAACACAG CCCCTGTGGT CCAGCCACGC 
TCCATCCAGG AGCTCGTTCG AGAATGTAGC CGTGGTAGGA CCTCAGACTT CAGGGGAGGC 
AGTCTGAGTG GGAACAGTGC CGCCAAGGTG GTACTCAGCC TTTCCACCCA GGCTGACAGG 
CTCTTTGACG ATGCTACAGA TAAGTTGAAC CTAACGGCCT TGGGAGGGTT CCTCTACCAG 
CTAAAGAAAG CGTCTCAGGC CCAGCTTTTC CACTCGGTGA CAGACACTGT CGATTACTCT 
CTGGCAATGC CAGGAGAGGT TAAGTCCACC CAGGACCGCA AAAGCGCTCT GCACCTGTTC 
CGCCTGGGGG ATGCCATGCT GAGGATCGTG AGAAGCAAAG CCCGACCCCT GCTCCACGTG 
ATGCGCTGCT GGAGCCTTGT GGCACCACAC CTGGTGGAGG CGGCCTGCCA CAAGGAGCGC 
CACGTGTCTC AGAAGGCTGT TTCCTTCATC CATGATATCC TGACCGAGGT TCTCACTGAC 
TGGAGTGAGC CGCCTCACTT CCACTTTAAT GAAGCGCTCT TCCGGCCCTT TGAGCGTATC 
ATGCAGTTGG AGCTGTGTGA CGAAGATGTC CAAGACCAGG TGGTCACCTC CATTGGGGAG 
CTGGTGGAGG TGTGCTCCAC GCAGATCCAG TCGGGATGGA GACCCCTGTT CAGTGCACTG 
GAAACAGTGC ACAGTGGGAA CAAGTCGGAG GTGAAGGAGT ACCTGGTTGG GGACTACGCC 
ATGGGAAAAG GCCAGGCTCC TGTGTTCGAC GTGTTTGAAG CCTTTCTCAA CACGGACAAC 
ATCCAGGTCT TTGCCAATGC AGCCACTAGT TACATCATGT GCCTTATGAA GTTTGTCAAA 
GGACTGGGGG AGGTGGACTG TAAAGAGATT GGAGACTGTG TCCCTGGAGT GGGAGCCACA 
TCCACAGACC TGTGCCTGCC TGCTCTGGAT TACCTCAGAC GCTGCTCTCA GCTTTTGGCC 
AAGATCTACA AAATGCCCTT AAAGCCAATA TTCCTCAGCG GGCGACTTGC CAGCTTGCCA 
CGAAGGCTTC AGGAGCAGTC AGCCAGCAGC GAGGATGGAA TTGAATCGGT CCTATCAGAT 
TTTGATGATG AAACTGGTCT GATAGAAGTT TGGATCATCC TGCTGGAGCA GCTAACAGCA 
GCTGTGTCCA ACTGTCCCCG GCAACACCAA CCACCAACCT TAGATTTACT CTTTGAGCTG 
TTGAGAGATG TCACCAAAAC ACCTGGCCCA GGGTTTGGAA TCTACGCAGT CGTTCACCTT 
CTCCTTCCTG TGATGTCTCT GTGGCTCCGT CGTAGCCACA AGGACCATTC CTACTGGGAT 
GTGGCATCTG CGAACTTCAA GCATGCCATC GGCCTGTCCT GCGAGCTGGT GGTGGAGCAC 
ATTCAGAGCT TCCTACACTC AGACATCAGG TACGAGAGCA TGATCAACAC CATGCTGAAG 
GATCTCTTTG AGCTGCTGGT GGCATGTGTG GCCAAACCCA CAGAAACCAT CTCCAGAGTG 
GGCTGTTCCT GCATTAGGTA TGTCCTCGTG ACTGCGGGTC CTGTGTTCAC TGAGGAAATG 
TGGCGACTTG CCTGCTGTGC CCTGCAGGAT GCCTTCTCTG CCACACTCAA GCCAGTGAAG 
GACCTGCTCG GCTGCTTCCA CAGTGGCACA GAGGGCTTCA GCGGAGAAGG CTGCCAGGTG 
CGGGTAGCAG CCCCATCCTC CTCCCCGAGT GCTGAGGCAG AGTACTGGCG CATCCGGGCC 
ATGGCCCAGC AGGTGTTTAT GTTGGACACC CAGGGCTCAC CAAAGACCCC AAACAACTTC 
GATCATGCTC AGTCCTGCCA GCTCATCATT GAACTGCCTC CTGATGAGAA ACCAAATGGG 
CACGCCAAGA AAAGTGTTTC TTTCAGGGAG ATCGTGGTGA GCTTGCTTTC TCACCAAGTA 
CTGCTCCAGA ACCTGTATGA CGTCCTGCTG GAAGAGTTTG TCAAAGGCCC CTCTCCAGGA 
GAGGAGAAGA CGACGGTGCA AGTGCCAGAC ACCAAGCTGG CTGGCTTTCT CAGATACATC 
TCCATGCAGA ACCTGGCTGT GATATTTGAC CTGCTGCTGG ACTCCTACAG GACTGCCCGA 
GAATTCGACA CGAGCCCAGG ACTGAAGTGT CTGCTGAAGA AAGTGTCCGG CATCGGAGGC 
GCCGCCAACC TGTACCGCCA GTCTGCCATG AGCTTTAACA TTTACTTTCA CGCCCTGGTT 
TGCGCAGTTC TCACCAACCA AGAAACCATC ACTGCCGAGC AGGTGAAGAA GGTCCTCTTC 
GACGAGGAGG AGAGGAGCTC CGATTCCTCC CAACAGTGCT CATCGGAGGA TGAAGACATC 
TTTGAGGAGA CGGCGCAGGT GAGCCCTCCG CGAGGCAAGG AGAAAAGGCA ATGGAGGGCA 
CGCCTGCCGT CGCTCAGCGT GCAGCCGGTG AGCAATGCAG ACTGGGTGTG GCTGGTCAAG 
AGGCTACACA AGCTGTGCAT GGAGCTCTGT AACCACTACA TCCAGATGCA CCTGGACCTG 
GAGAGCAGCT TAGAGGAGCC TCCCGCCTTC AAAAGCGACC CGTTCTTCAT CCTGCCATCC 
TTCCAGTCTG AGTCGTCCAC ACCATCCACG GGTGGCTTCT CCGGGAAAAA CACGCCCTCG 
GAAGATGACC GCAGAGAGCA TCTAAGTGAG CCCCTAAGTC TGAGGGTGGG CAGTGGGGAC 
GTGCTGATGC TGCCCCCCAG TCCCAAGACA GAGAAGAAGG ATCCTGGCCG CAAGAAAGAG 
TGGTGGGAGA GCGCGGGGAA CAAGATTTGC ACCATGGCTG CCGACAAAAC CATCTCAAAG 
CTGATGACCG AGTACAAGAA GAGGAGGCAG CCGCACAACC TGCCTGCCTT CCCCAAGGAA 
GTCAAGGCAG ATAAGAAGGG CGAGCCCCTG GGGCCTCGGG GTCCTGATTC CCCGCTGCTT 
CAGCGGCCAC AGCATCTCAT GGACCAAGGA CAGATGCGCC ATTCCTTCAG TGCAGGCCCA 
GAGCTGTTGC GGCAGGAGAA GAGACCCCGC TCAGGCTCCA CCGGGAGCTC CCTGAGTGTC 
TCGGTGCGAG ACGCAGAGGC ACAGATCCAG GCGTGGACCA ACATGGTGCT AACAGTTCTC 
AATCAGATTC AAATCCTTCC AGACCAGACA TTCACGGCCC TCCAGCCAGC AGTGTTCCCG 
TGCATCAGCC AGCTGACCTG TCATGTGACT GACATCAGAG TCCGTCAAGC TGTAAGGGAA 
TGGCTGGGAA GAGTGGGCCG TGTCTATGAT ATCATCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005065899.1
CDS	86..6607
Translation	MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTIVKIPPHLLREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSRETDSDEKQLLNQILNAVKVTPLLNEDLQVEVMKVLLCITYTSTFDMNGSAVLKIAEVCIQTYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFGSQGSTVEALCDDVVSVLAVLCEKLQASINDSQQLQLLYLECILSMLSSSSSCMYLHRGFTDLIWKSLCPALIVILGNPIHDKTITSAHSSSSSSSTNVESDSASPGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSTELEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLNESQVQLLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEHTPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHRNGFKSPAIQEGKETMCKVSEQEAIDQPDVVQRSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDNCSLADEEQTPRDYMGHRSLRTAALSLKLLKNQEADQHSARLFVQSLEGLLPRLLALSSVEEVDTALQNFASTFCSGMMHSPGFDGGSSLSFQMLMNADSLYSAAHCALLLNLKLSHGDYYRKRPTLAPGMMKEFMKQVQTSGVLMVFSQAWIEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASASMESPFTQSRRIDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEEREAQEPSDAIAQVKLKVEQKLEQMGKVQGVWLHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGASQPPLTIRQPQKTSGSSGLLGDLECEGSSQEQSLEQGPSLNTAPVVQPRSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLFDDATDKLNLTALGGFLYQLKKASQAQLFHSVTDTVDYSLAMPGEVKSTQDRKSALHLFRLGDAMLRIVRSKARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQLELCDEDVQDQVVTSIGELVEVCSTQIQSGWRPLFSALETVHSGNKSEVKEYLVGDYAMGKGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGVGATSTDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDETGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLRRSHKDHSYWDVASANFKHAIGLSCELVVEHIQSFLHSDIRYESMINTMLKDLFELLVACVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAFSATLKPVKDLLGCFHSGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQGSPKTPNNFDHAQSCQLIIELPPDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDVLLEEFVKGPSPGEEKTTVQVPDTKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFDEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNADWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPPAFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDDRREHLSEPLSLRVGSGDVLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHNLPAFPKEVKADKKGEPLGPRGPDSPLLQRPQHLMDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIII

Target ORF information:

RefSeq Version	XM_005065842.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005065842.2

ORF Insert Sequence:

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGATATT 
GATGGCTTAG AGAGCAGCGC AATTGGTGGC CAGTTGATGG CATCAGCTTC AATGGAGTCA 
CCTTTCACCC AGAGCAGGAG AATTGATGAC TCCACTGTAG CAGGTGTGGC ATTTGCTCGT 
TATATTTTGG TGGGCTGCTG GAAGAACCTG ATTGATACTC TGTCAACCCC CTTGACTGGT 
CGAATGGCAG GAAGCTCCAA GGGGCTGGCC TTCATCCTCG GAGCTGAGGG CATAAAAGAG 
CAGAACCAGA AGGAGCGAGA TGCCATCTGC ATGAGCCTTG ACGGGCTGCG CAAAGCTGCA 
CGACTGAGCT GTGCACTAGG GGTTGCTGCT AACTGCGCTT CAGCACTTGC CCAGATGGCG 
GCTGCTTCCT GTGTCCAGGA AGAGAAAGAA GAAAGGGAAG CCCAGGAGCC CAGTGATGCC 
ATAGCACAAG TGAAACTAAA AGTGGAGCAG AAACTGGAGC AGATGGGGAA GGTGCAGGGG 
GTGTGGCTGC ACACAGCCCA CGTCTTGTGC ATGGATGCCA TCCTCAGCGT AGGCCTGGAG 
ATGGGAAGCC ACAACCCGGA CTGCTGGCCA CACGTGTTCA GGGTGTGTGA GTATGTGGGC 
ACATTGGAGC ACACTCACTT CAGTGATGGT GCCTCCCAGC CCCCTCTGAC CATCCGTCAG 
CCACAGAAGA CATCTGGGAG CTCTGGCCTC CTGGGGGACC TTGAGTGTGA GGGTTCATCT 
CAGGAGCAGA GCCTGGAGCA GGGCCCCTCC CTGAACACAG CCCCTGTGGT CCAGCCACGC 
TCCATCCAGG AGCTCGTTCG AGAATGTAGC CGTGGTAGGA CCTCAGACTT CAGGGGAGGC 
AGTCTGAGTG GGAACAGTGC CGCCAAGGTG GTACTCAGCC TTTCCACCCA GGCTGACAGG 
CTCTTTGACG ATGCTACAGA TAAGTTGAAC CTAACGGCCT TGGGAGGGTT CCTCTACCAG 
CTAAAGAAAG CGTCTCAGGC CCAGCTTTTC CACTCGGTGA CAGACACTGT CGATTACTCT 
CTGGCAATGC CAGGAGAGGT TAAGTCCACC CAGGACCGCA AAAGCGCTCT GCACCTGTTC 
CGCCTGGGGG ATGCCATGCT GAGGATCGTG AGAAGCAAAG CCCGACCCCT GCTCCACGTG 
ATGCGCTGCT GGAGCCTTGT GGCACCACAC CTGGTGGAGG CGGCCTGCCA CAAGGAGCGC 
CACGTGTCTC AGAAGGCTGT TTCCTTCATC CATGATATCC TGACCGAGGT TCTCACTGAC 
TGGAGTGAGC CGCCTCACTT CCACTTTAAT GAAGCGCTCT TCCGGCCCTT TGAGCGTATC 
ATGCAGTTGG AGCTGTGTGA CGAAGATGTC CAAGACCAGG TGGTCACCTC CATTGGGGAG 
CTGGTGGAGG TGTGCTCCAC GCAGATCCAG TCGGGATGGA GACCCCTGTT CAGTGCACTG 
GAAACAGTGC ACAGTGGGAA CAAGTCGGAG GTGAAGGAGT ACCTGGTTGG GGACTACGCC 
ATGGGAAAAG GCCAGGCTCC TGTGTTCGAC GTGTTTGAAG CCTTTCTCAA CACGGACAAC 
ATCCAGGTCT TTGCCAATGC AGCCACTAGT TACATCATGT GCCTTATGAA GTTTGTCAAA 
GGACTGGGGG AGGTGGACTG TAAAGAGATT GGAGACTGTG TCCCTGGAGT GGGAGCCACA 
TCCACAGACC TGTGCCTGCC TGCTCTGGAT TACCTCAGAC GCTGCTCTCA GCTTTTGGCC 
AAGATCTACA AAATGCCCTT AAAGCCAATA TTCCTCAGCG GGCGACTTGC CAGCTTGCCA 
CGAAGGCTTC AGGAGCAGTC AGCCAGCAGC GAGGATGGAA TTGAATCGGT CCTATCAGAT 
TTTGATGATG AAACTGGTCT GATAGAAGTT TGGATCATCC TGCTGGAGCA GCTAACAGCA 
GCTGTGTCCA ACTGTCCCCG GCAACACCAA CCACCAACCT TAGATTTACT CTTTGAGCTG 
TTGAGAGATG TCACCAAAAC ACCTGGCCCA GGGTTTGGAA TCTACGCAGT CGTTCACCTT 
CTCCTTCCTG TGATGTCTCT GTGGCTCCGT CGTAGCCACA AGGACCATTC CTACTGGGAT 
GTGGCATCTG CGAACTTCAA GCATGCCATC GGCCTGTCCT GCGAGCTGGT GGTGGAGCAC 
ATTCAGAGCT TCCTACACTC AGACATCAGG TACGAGAGCA TGATCAACAC CATGCTGAAG 
GATCTCTTTG AGCTGCTGGT GGCATGTGTG GCCAAACCCA CAGAAACCAT CTCCAGAGTG 
GGCTGTTCCT GCATTAGGTA TGTCCTCGTG ACTGCGGGTC CTGTGTTCAC TGAGGAAATG 
TGGCGACTTG CCTGCTGTGC CCTGCAGGAT GCCTTCTCTG CCACACTCAA GCCAGTGAAG 
GACCTGCTCG GCTGCTTCCA CAGTGGCACA GAGGGCTTCA GCGGAGAAGG CTGCCAGGTG 
CGGGTAGCAG CCCCATCCTC CTCCCCGAGT GCTGAGGCAG AGTACTGGCG CATCCGGGCC 
ATGGCCCAGC AGGTGTTTAT GTTGGACACC CAGGGCTCAC CAAAGACCCC AAACAACTTC 
GATCATGCTC AGTCCTGCCA GCTCATCATT GAACTGCCTC CTGATGAGAA ACCAAATGGG 
CACGCCAAGA AAAGTGTTTC TTTCAGGGAG ATCGTGGTGA GCTTGCTTTC TCACCAAGTA 
CTGCTCCAGA ACCTGTATGA CGTCCTGCTG GAAGAGTTTG TCAAAGGCCC CTCTCCAGGA 
GAGGAGAAGA CGACGGTGCA AGTGCCAGAC ACCAAGCTGG CTGGCTTTCT CAGATACATC 
TCCATGCAGA ACCTGGCTGT GATATTTGAC CTGCTGCTGG ACTCCTACAG GACTGCCCGA 
GAATTCGACA CGAGCCCAGG ACTGAAGTGT CTGCTGAAGA AAGTGTCCGG CATCGGAGGC 
GCCGCCAACC TGTACCGCCA GTCTGCCATG AGCTTTAACA TTTACTTTCA CGCCCTGGTT 
TGCGCAGTTC TCACCAACCA AGAAACCATC ACTGCCGAGC AGGTGAAGAA GGTCCTCTTC 
GACGAGGAGG AGAGGAGCTC CGATTCCTCC CAACAGTGCT CATCGGAGGA TGAAGACATC 
TTTGAGGAGA CGGCGCAGGT GAGCCCTCCG CGAGGCAAGG AGAAAAGGCA ATGGAGGGCA 
CGCCTGCCGT CGCTCAGCGT GCAGCCGGTG AGCAATGCAG ACTGGGTGTG GCTGGTCAAG 
AGGCTACACA AGCTGTGCAT GGAGCTCTGT AACCACTACA TCCAGATGCA CCTGGACCTG 
GAGAGCAGCT TAGAGGAGCC TCCCGCCTTC AAAAGCGACC CGTTCTTCAT CCTGCCATCC 
TTCCAGTCTG AGTCGTCCAC ACCATCCACG GGTGGCTTCT CCGGGAAAAA CACGCCCTCG 
GAAGATGACC GCAGAGAGCA TCTAAGTGAG CCCCTAAGTC TGAGGGTGGG CAGTGGGGAC 
GTGCTGATGC TGCCCCCCAG TCCCAAGACA GAGAAGAAGG ATCCTGGCCG CAAGAAAGAG 
TGGTGGGAGA GCGCGGGGAA CAAGATTTGC ACCATGGCTG CCGACAAAAC CATCTCAAAG 
CTGATGACCG AGTACAAGAA GAGGAGGCAG CCGCACAACC TGCCTGCCTT CCCCAAGGAA 
GTCAAGGCAG ATAAGAAGGG CGAGCCCCTG GGGCCTCGGG GTCCTGATTC CCCGCTGCTT 
CAGCGGCCAC AGCATCTCAT GGACCAAGGA CAGATGCGCC ATTCCTTCAG TGCAGGCCCA 
GAGCTGTTGC GGCAGGAGAA GAGACCCCGC TCAGGCTCCA CCGGGAGCTC CCTGAGTGTC 
TCGGTGCGAG ACGCAGAGGC ACAGATCCAG GCGTGGACCA ACATGGTGCT AACAGTTCTC 
AATCAGATTC AAATCCTTCC AGACCAGACA TTCACGGCCC TCCAGCCAGC AGTGTTCCCG 
TGCATCAGCC AGCTGACCTG TCATGTGACT GACATCAGAG TCCGTCAAGC TGTAAGGGAA 
TGGCTGGGAA GAGTGGGCCG TGTCTATGAT ATCATCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef3 ( XM_005065842.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005065899.1 Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.

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CloneID	OMe118813
Clone ID Related Accession (Same CDS sequence)	XM_005065842.2 , XM_005065842.3
Accession Version	XM_005065842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801608.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005065842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGATATT 
GATGGCTTAG AGAGCAGCGC AATTGGTGGC CAGTTGATGG CATCAGCTTC AATGGAGTCA 
CCTTTCACCC AGAGCAGGAG AATTGATGAC TCCACTGTAG CAGGTGTGGC ATTTGCTCGT 
TATATTTTGG TGGGCTGCTG GAAGAACCTG ATTGATACTC TGTCAACCCC CTTGACTGGT 
CGAATGGCAG GAAGCTCCAA GGGGCTGGCC TTCATCCTCG GAGCTGAGGG CATAAAAGAG 
CAGAACCAGA AGGAGCGAGA TGCCATCTGC ATGAGCCTTG ACGGGCTGCG CAAAGCTGCA 
CGACTGAGCT GTGCACTAGG GGTTGCTGCT AACTGCGCTT CAGCACTTGC CCAGATGGCG 
GCTGCTTCCT GTGTCCAGGA AGAGAAAGAA GAAAGGGAAG CCCAGGAGCC CAGTGATGCC 
ATAGCACAAG TGAAACTAAA AGTGGAGCAG AAACTGGAGC AGATGGGGAA GGTGCAGGGG 
GTGTGGCTGC ACACAGCCCA CGTCTTGTGC ATGGATGCCA TCCTCAGCGT AGGCCTGGAG 
ATGGGAAGCC ACAACCCGGA CTGCTGGCCA CACGTGTTCA GGGTGTGTGA GTATGTGGGC 
ACATTGGAGC ACACTCACTT CAGTGATGGT GCCTCCCAGC CCCCTCTGAC CATCCGTCAG 
CCACAGAAGA CATCTGGGAG CTCTGGCCTC CTGGGGGACC TTGAGTGTGA GGGTTCATCT 
CAGGAGCAGA GCCTGGAGCA GGGCCCCTCC CTGAACACAG CCCCTGTGGT CCAGCCACGC 
TCCATCCAGG AGCTCGTTCG AGAATGTAGC CGTGGTAGGA CCTCAGACTT CAGGGGAGGC 
AGTCTGAGTG GGAACAGTGC CGCCAAGGTG GTACTCAGCC TTTCCACCCA GGCTGACAGG 
CTCTTTGACG ATGCTACAGA TAAGTTGAAC CTAACGGCCT TGGGAGGGTT CCTCTACCAG 
CTAAAGAAAG CGTCTCAGGC CCAGCTTTTC CACTCGGTGA CAGACACTGT CGATTACTCT 
CTGGCAATGC CAGGAGAGGT TAAGTCCACC CAGGACCGCA AAAGCGCTCT GCACCTGTTC 
CGCCTGGGGG ATGCCATGCT GAGGATCGTG AGAAGCAAAG CCCGACCCCT GCTCCACGTG 
ATGCGCTGCT GGAGCCTTGT GGCACCACAC CTGGTGGAGG CGGCCTGCCA CAAGGAGCGC 
CACGTGTCTC AGAAGGCTGT TTCCTTCATC CATGATATCC TGACCGAGGT TCTCACTGAC 
TGGAGTGAGC CGCCTCACTT CCACTTTAAT GAAGCGCTCT TCCGGCCCTT TGAGCGTATC 
ATGCAGTTGG AGCTGTGTGA CGAAGATGTC CAAGACCAGG TGGTCACCTC CATTGGGGAG 
CTGGTGGAGG TGTGCTCCAC GCAGATCCAG TCGGGATGGA GACCCCTGTT CAGTGCACTG 
GAAACAGTGC ACAGTGGGAA CAAGTCGGAG GTGAAGGAGT ACCTGGTTGG GGACTACGCC 
ATGGGAAAAG GCCAGGCTCC TGTGTTCGAC GTGTTTGAAG CCTTTCTCAA CACGGACAAC 
ATCCAGGTCT TTGCCAATGC AGCCACTAGT TACATCATGT GCCTTATGAA GTTTGTCAAA 
GGACTGGGGG AGGTGGACTG TAAAGAGATT GGAGACTGTG TCCCTGGAGT GGGAGCCACA 
TCCACAGACC TGTGCCTGCC TGCTCTGGAT TACCTCAGAC GCTGCTCTCA GCTTTTGGCC 
AAGATCTACA AAATGCCCTT AAAGCCAATA TTCCTCAGCG GGCGACTTGC CAGCTTGCCA 
CGAAGGCTTC AGGAGCAGTC AGCCAGCAGC GAGGATGGAA TTGAATCGGT CCTATCAGAT 
TTTGATGATG AAACTGGTCT GATAGAAGTT TGGATCATCC TGCTGGAGCA GCTAACAGCA 
GCTGTGTCCA ACTGTCCCCG GCAACACCAA CCACCAACCT TAGATTTACT CTTTGAGCTG 
TTGAGAGATG TCACCAAAAC ACCTGGCCCA GGGTTTGGAA TCTACGCAGT CGTTCACCTT 
CTCCTTCCTG TGATGTCTCT GTGGCTCCGT CGTAGCCACA AGGACCATTC CTACTGGGAT 
GTGGCATCTG CGAACTTCAA GCATGCCATC GGCCTGTCCT GCGAGCTGGT GGTGGAGCAC 
ATTCAGAGCT TCCTACACTC AGACATCAGG TACGAGAGCA TGATCAACAC CATGCTGAAG 
GATCTCTTTG AGCTGCTGGT GGCATGTGTG GCCAAACCCA CAGAAACCAT CTCCAGAGTG 
GGCTGTTCCT GCATTAGGTA TGTCCTCGTG ACTGCGGGTC CTGTGTTCAC TGAGGAAATG 
TGGCGACTTG CCTGCTGTGC CCTGCAGGAT GCCTTCTCTG CCACACTCAA GCCAGTGAAG 
GACCTGCTCG GCTGCTTCCA CAGTGGCACA GAGGGCTTCA GCGGAGAAGG CTGCCAGGTG 
CGGGTAGCAG CCCCATCCTC CTCCCCGAGT GCTGAGGCAG AGTACTGGCG CATCCGGGCC 
ATGGCCCAGC AGGTGTTTAT GTTGGACACC CAGGGCTCAC CAAAGACCCC AAACAACTTC 
GATCATGCTC AGTCCTGCCA GCTCATCATT GAACTGCCTC CTGATGAGAA ACCAAATGGG 
CACGCCAAGA AAAGTGTTTC TTTCAGGGAG ATCGTGGTGA GCTTGCTTTC TCACCAAGTA 
CTGCTCCAGA ACCTGTATGA CGTCCTGCTG GAAGAGTTTG TCAAAGGCCC CTCTCCAGGA 
GAGGAGAAGA CGACGGTGCA AGTGCCAGAC ACCAAGCTGG CTGGCTTTCT CAGATACATC 
TCCATGCAGA ACCTGGCTGT GATATTTGAC CTGCTGCTGG ACTCCTACAG GACTGCCCGA 
GAATTCGACA CGAGCCCAGG ACTGAAGTGT CTGCTGAAGA AAGTGTCCGG CATCGGAGGC 
GCCGCCAACC TGTACCGCCA GTCTGCCATG AGCTTTAACA TTTACTTTCA CGCCCTGGTT 
TGCGCAGTTC TCACCAACCA AGAAACCATC ACTGCCGAGC AGGTGAAGAA GGTCCTCTTC 
GACGAGGAGG AGAGGAGCTC CGATTCCTCC CAACAGTGCT CATCGGAGGA TGAAGACATC 
TTTGAGGAGA CGGCGCAGGT GAGCCCTCCG CGAGGCAAGG AGAAAAGGCA ATGGAGGGCA 
CGCCTGCCGT CGCTCAGCGT GCAGCCGGTG AGCAATGCAG ACTGGGTGTG GCTGGTCAAG 
AGGCTACACA AGCTGTGCAT GGAGCTCTGT AACCACTACA TCCAGATGCA CCTGGACCTG 
GAGAGCAGCT TAGAGGAGCC TCCCGCCTTC AAAAGCGACC CGTTCTTCAT CCTGCCATCC 
TTCCAGTCTG AGTCGTCCAC ACCATCCACG GGTGGCTTCT CCGGGAAAAA CACGCCCTCG 
GAAGATGACC GCAGAGAGCA TCTAAGTGAG CCCCTAAGTC TGAGGGTGGG CAGTGGGGAC 
GTGCTGATGC TGCCCCCCAG TCCCAAGACA GAGAAGAAGG ATCCTGGCCG CAAGAAAGAG 
TGGTGGGAGA GCGCGGGGAA CAAGATTTGC ACCATGGCTG CCGACAAAAC CATCTCAAAG 
CTGATGACCG AGTACAAGAA GAGGAGGCAG CCGCACAACC TGCCTGCCTT CCCCAAGGAA 
GTCAAGGCAG ATAAGAAGGG CGAGCCCCTG GGGCCTCGGG GTCCTGATTC CCCGCTGCTT 
CAGCGGCCAC AGCATCTCAT GGACCAAGGA CAGATGCGCC ATTCCTTCAG TGCAGGCCCA 
GAGCTGTTGC GGCAGGAGAA GAGACCCCGC TCAGGCTCCA CCGGGAGCTC CCTGAGTGTC 
TCGGTGCGAG ACGCAGAGGC ACAGATCCAG GCGTGGACCA ACATGGTGCT AACAGTTCTC 
AATCAGATTC AAATCCTTCC AGACCAGACA TTCACGGCCC TCCAGCCAGC AGTGTTCCCG 
TGCATCAGCC AGCTGACCTG TCATGTGACT GACATCAGAG TCCGTCAAGC TGTAAGGGAA 
TGGCTGGGAA GAGTGGGCCG TGTCTATGAT ATCATCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005065899.1
CDS	102..6623
Translation	MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTIVKIPPHLLREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSRETDSDEKQLLNQILNAVKVTPLLNEDLQVEVMKVLLCITYTSTFDMNGSAVLKIAEVCIQTYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFGSQGSTVEALCDDVVSVLAVLCEKLQASINDSQQLQLLYLECILSMLSSSSSCMYLHRGFTDLIWKSLCPALIVILGNPIHDKTITSAHSSSSSSSTNVESDSASPGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSTELEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLNESQVQLLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEHTPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHRNGFKSPAIQEGKETMCKVSEQEAIDQPDVVQRSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDNCSLADEEQTPRDYMGHRSLRTAALSLKLLKNQEADQHSARLFVQSLEGLLPRLLALSSVEEVDTALQNFASTFCSGMMHSPGFDGGSSLSFQMLMNADSLYSAAHCALLLNLKLSHGDYYRKRPTLAPGMMKEFMKQVQTSGVLMVFSQAWIEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASASMESPFTQSRRIDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEEREAQEPSDAIAQVKLKVEQKLEQMGKVQGVWLHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGASQPPLTIRQPQKTSGSSGLLGDLECEGSSQEQSLEQGPSLNTAPVVQPRSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLFDDATDKLNLTALGGFLYQLKKASQAQLFHSVTDTVDYSLAMPGEVKSTQDRKSALHLFRLGDAMLRIVRSKARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQLELCDEDVQDQVVTSIGELVEVCSTQIQSGWRPLFSALETVHSGNKSEVKEYLVGDYAMGKGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGVGATSTDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDETGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLRRSHKDHSYWDVASANFKHAIGLSCELVVEHIQSFLHSDIRYESMINTMLKDLFELLVACVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAFSATLKPVKDLLGCFHSGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQGSPKTPNNFDHAQSCQLIIELPPDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDVLLEEFVKGPSPGEEKTTVQVPDTKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFDEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNADWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPPAFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDDRREHLSEPLSLRVGSGDVLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHNLPAFPKEVKADKKGEPLGPRGPDSPLLQRPQHLMDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIII

Target ORF information:

RefSeq Version	XM_005065842.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus ARFGEF family member 3 (Arfgef3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005065842.3

ORF Insert Sequence:

ATGGAAGAGA TCCTGAGGAA GCTGCAGAGG GACGCATCCG GGAGCAAATA CAAAGCCATC 
AAGGAGAGCT GCACCTGGGC CTTGGAAACT CTAGGTGGCC TGGACACCAT TGTGAAGATC 
CCTCCCCACT TGCTGAGGGA GAAATGCTTG CTGCCTCTCC AGTTGGCTTT GGAATCGAAG 
AATGTGAAGC TGGCCCAACA TGCTTTGGCT GGGATGCAGA AGCTCCTGTC AGAAGAGAGG 
TTTGTATCCA GGGAGACAGA CTCTGATGAG AAGCAACTGC TCAATCAGAT CCTGAACGCC 
GTGAAAGTGA CACCTTTGCT CAATGAGGAC CTACAGGTGG AAGTGATGAA GGTTTTGCTG 
TGCATCACCT ACACATCAAC ATTTGACATG AATGGGAGTG CTGTGCTGAA GATTGCAGAG 
GTGTGCATCC AGACCTACAC ATGCAGCTGC CACCAGCGCA GTATCAACAC TGCCGTGCGC 
GCGACTCTCA GCCAGATGTT GGGTGACCTG ACTTTGCAGT TACGACAGAG GCAGGAGAAC 
ACGATAATTG AAAACCCAGA TGCTCCACAG GAATTCGGGA GTCAAGGCTC AACAGTAGAG 
GCCCTTTGTG ATGACGTTGT CTCTGTCCTT GCTGTCCTGT GTGAGAAGCT CCAAGCCTCC 
ATAAACGACA GCCAGCAGCT GCAGCTTCTG TACCTGGAAT GCATTCTCTC CATGCTCAGC 
AGCTCCTCCT CCTGCATGTA CCTGCACAGG GGCTTCACTG ACCTCATCTG GAAAAGTCTC 
TGCCCTGCTC TCATTGTGAT CCTGGGGAAC CCCATTCACG ACAAAACCAT CACCTCTGCT 
CACAGCAGCA GCAGCAGCAG CAGCACCAAC GTGGAGTCGG ACTCTGCCTC TCCTGGTGTG 
TCTGACCATG GCCGAGGCTC AGGTTGCTCC TGCACTGCAC CCACCCTCAG TGGACCTGTG 
GCCCGCACCA TCTATTACCT TGCAGCTGAG CTCGTCCGCT TAGTGGGGTC AGTGGATTCC 
ATGAAGCCGG TGCTCCAGTC CCTCTATCAC CGTGTGCTCC TGTATCCCCC GCCCCAGCAC 
CGCGTGGAAG CCATCAAAAT CATGAAAGAG ATACTCGGGA GCCCCCAGCG GCTCTATGAC 
TTGGCTGGAC CCAGCTCTAC TGAGTTGGAA CCGAGGAAAA GATCTATTTC CAAAAGAAAG 
TCTCACTTGG ATCTTCTCAA ACTCATCATG GATGGCATGA CAGAAGCATG CATCAAAGGT 
GGCATCGAAG CCTGTTACGC TGCCGTGTCT TGTGTCTGCA CCTTGCTTGG GGCCCTGGAT 
GAACTCAGCC AGGGGAAGGG CTTGAATGAG AGTCAAGTCC AACTGCTGCT TCTGCGCCTT 
GAAGAGCTGA GGGATGGGGC CGAGTCAAGC CGGGATTCCA TGGAGATTAA TGAGGCTGAC 
TTCCGCTGGC AGCGGCGAGT GCTGTCTTCA GAGCACACGC CATGGGAGTC AGGTAACGAG 
AGAAGCCCAG ACATCAGCAT CAGTGTCACC ACAGACACAG GCCAGACTAC CTTGGAGGGA 
GAATTGGGTC AGACAACACC AGAGGACCAC AGGAATGGGT TCAAGTCACC AGCTATTCAG 
GAAGGCAAGG AGACCATGTG CAAAGTGTCA GAACAAGAGG CCATAGACCA GCCCGATGTA 
GTTCAGAGAA GCCACACAGT CCCTTACCCT GACATCACTA ACTTCCTGTC AGTAGACTGC 
AGGACAAGGT CCTACGGATC CAGGTACAGT GAGAGCAACT TCAGCGTAGA TGACCAAGAC 
CTGTCGAGGA CAGAATTTGA TTCGTGTGAT CAGTACTCCA TGGCAGCAGA AAAAGACTCG 
GGTAGGTCTG ATGTGTCAGA CATTGGGTCG GACAACTGTT CACTGGCTGA TGAAGAGCAG 
ACCCCCCGGG ACTACATGGG CCATCGATCC CTGCGCACGG CTGCTCTGTC TCTCAAACTG 
CTGAAGAACC AGGAGGCCGA CCAGCACAGC GCACGGCTGT TTGTGCAGTC CCTCGAGGGC 
CTCCTTCCCC GCCTCCTAGC CCTGTCCAGT GTGGAAGAGG TGGACACCGC CCTTCAGAAC 
TTTGCCTCCA CTTTCTGCTC AGGCATGATG CATTCTCCCG GCTTCGATGG AGGGAGCAGC 
TTGAGCTTCC AGATGTTGAT GAATGCAGAC AGCTTGTACA GTGCGGCACA CTGTGCCCTA 
CTGCTCAACC TGAAGCTCTC CCACGGTGAC TACTACCGGA AGCGGCCAAC CCTGGCACCA 
GGCATGATGA AAGAGTTCAT GAAACAGGTG CAGACCAGTG GCGTGCTGAT GGTCTTCTCC 
CAGGCCTGGA TCGAGGAGCT ATACCATCAG GTGCTGGACA GAAACATGCT GGGAGAAGCT 
GGCTACTGGG GCAGCCCTGA GGACAACAGC CTTCCCCTCA TCACCATGCT CACAGATATT 
GATGGCTTAG AGAGCAGCGC AATTGGTGGC CAGTTGATGG CATCAGCTTC AATGGAGTCA 
CCTTTCACCC AGAGCAGGAG AATTGATGAC TCCACTGTAG CAGGTGTGGC ATTTGCTCGT 
TATATTTTGG TGGGCTGCTG GAAGAACCTG ATTGATACTC TGTCAACCCC CTTGACTGGT 
CGAATGGCAG GAAGCTCCAA GGGGCTGGCC TTCATCCTCG GAGCTGAGGG CATAAAAGAG 
CAGAACCAGA AGGAGCGAGA TGCCATCTGC ATGAGCCTTG ACGGGCTGCG CAAAGCTGCA 
CGACTGAGCT GTGCACTAGG GGTTGCTGCT AACTGCGCTT CAGCACTTGC CCAGATGGCG 
GCTGCTTCCT GTGTCCAGGA AGAGAAAGAA GAAAGGGAAG CCCAGGAGCC CAGTGATGCC 
ATAGCACAAG TGAAACTAAA AGTGGAGCAG AAACTGGAGC AGATGGGGAA GGTGCAGGGG 
GTGTGGCTGC ACACAGCCCA CGTCTTGTGC ATGGATGCCA TCCTCAGCGT AGGCCTGGAG 
ATGGGAAGCC ACAACCCGGA CTGCTGGCCA CACGTGTTCA GGGTGTGTGA GTATGTGGGC 
ACATTGGAGC ACACTCACTT CAGTGATGGT GCCTCCCAGC CCCCTCTGAC CATCCGTCAG 
CCACAGAAGA CATCTGGGAG CTCTGGCCTC CTGGGGGACC TTGAGTGTGA GGGTTCATCT 
CAGGAGCAGA GCCTGGAGCA GGGCCCCTCC CTGAACACAG CCCCTGTGGT CCAGCCACGC 
TCCATCCAGG AGCTCGTTCG AGAATGTAGC CGTGGTAGGA CCTCAGACTT CAGGGGAGGC 
AGTCTGAGTG GGAACAGTGC CGCCAAGGTG GTACTCAGCC TTTCCACCCA GGCTGACAGG 
CTCTTTGACG ATGCTACAGA TAAGTTGAAC CTAACGGCCT TGGGAGGGTT CCTCTACCAG 
CTAAAGAAAG CGTCTCAGGC CCAGCTTTTC CACTCGGTGA CAGACACTGT CGATTACTCT 
CTGGCAATGC CAGGAGAGGT TAAGTCCACC CAGGACCGCA AAAGCGCTCT GCACCTGTTC 
CGCCTGGGGG ATGCCATGCT GAGGATCGTG AGAAGCAAAG CCCGACCCCT GCTCCACGTG 
ATGCGCTGCT GGAGCCTTGT GGCACCACAC CTGGTGGAGG CGGCCTGCCA CAAGGAGCGC 
CACGTGTCTC AGAAGGCTGT TTCCTTCATC CATGATATCC TGACCGAGGT TCTCACTGAC 
TGGAGTGAGC CGCCTCACTT CCACTTTAAT GAAGCGCTCT TCCGGCCCTT TGAGCGTATC 
ATGCAGTTGG AGCTGTGTGA CGAAGATGTC CAAGACCAGG TGGTCACCTC CATTGGGGAG 
CTGGTGGAGG TGTGCTCCAC GCAGATCCAG TCGGGATGGA GACCCCTGTT CAGTGCACTG 
GAAACAGTGC ACAGTGGGAA CAAGTCGGAG GTGAAGGAGT ACCTGGTTGG GGACTACGCC 
ATGGGAAAAG GCCAGGCTCC TGTGTTCGAC GTGTTTGAAG CCTTTCTCAA CACGGACAAC 
ATCCAGGTCT TTGCCAATGC AGCCACTAGT TACATCATGT GCCTTATGAA GTTTGTCAAA 
GGACTGGGGG AGGTGGACTG TAAAGAGATT GGAGACTGTG TCCCTGGAGT GGGAGCCACA 
TCCACAGACC TGTGCCTGCC TGCTCTGGAT TACCTCAGAC GCTGCTCTCA GCTTTTGGCC 
AAGATCTACA AAATGCCCTT AAAGCCAATA TTCCTCAGCG GGCGACTTGC CAGCTTGCCA 
CGAAGGCTTC AGGAGCAGTC AGCCAGCAGC GAGGATGGAA TTGAATCGGT CCTATCAGAT 
TTTGATGATG AAACTGGTCT GATAGAAGTT TGGATCATCC TGCTGGAGCA GCTAACAGCA 
GCTGTGTCCA ACTGTCCCCG GCAACACCAA CCACCAACCT TAGATTTACT CTTTGAGCTG 
TTGAGAGATG TCACCAAAAC ACCTGGCCCA GGGTTTGGAA TCTACGCAGT CGTTCACCTT 
CTCCTTCCTG TGATGTCTCT GTGGCTCCGT CGTAGCCACA AGGACCATTC CTACTGGGAT 
GTGGCATCTG CGAACTTCAA GCATGCCATC GGCCTGTCCT GCGAGCTGGT GGTGGAGCAC 
ATTCAGAGCT TCCTACACTC AGACATCAGG TACGAGAGCA TGATCAACAC CATGCTGAAG 
GATCTCTTTG AGCTGCTGGT GGCATGTGTG GCCAAACCCA CAGAAACCAT CTCCAGAGTG 
GGCTGTTCCT GCATTAGGTA TGTCCTCGTG ACTGCGGGTC CTGTGTTCAC TGAGGAAATG 
TGGCGACTTG CCTGCTGTGC CCTGCAGGAT GCCTTCTCTG CCACACTCAA GCCAGTGAAG 
GACCTGCTCG GCTGCTTCCA CAGTGGCACA GAGGGCTTCA GCGGAGAAGG CTGCCAGGTG 
CGGGTAGCAG CCCCATCCTC CTCCCCGAGT GCTGAGGCAG AGTACTGGCG CATCCGGGCC 
ATGGCCCAGC AGGTGTTTAT GTTGGACACC CAGGGCTCAC CAAAGACCCC AAACAACTTC 
GATCATGCTC AGTCCTGCCA GCTCATCATT GAACTGCCTC CTGATGAGAA ACCAAATGGG 
CACGCCAAGA AAAGTGTTTC TTTCAGGGAG ATCGTGGTGA GCTTGCTTTC TCACCAAGTA 
CTGCTCCAGA ACCTGTATGA CGTCCTGCTG GAAGAGTTTG TCAAAGGCCC CTCTCCAGGA 
GAGGAGAAGA CGACGGTGCA AGTGCCAGAC ACCAAGCTGG CTGGCTTTCT CAGATACATC 
TCCATGCAGA ACCTGGCTGT GATATTTGAC CTGCTGCTGG ACTCCTACAG GACTGCCCGA 
GAATTCGACA CGAGCCCAGG ACTGAAGTGT CTGCTGAAGA AAGTGTCCGG CATCGGAGGC 
GCCGCCAACC TGTACCGCCA GTCTGCCATG AGCTTTAACA TTTACTTTCA CGCCCTGGTT 
TGCGCAGTTC TCACCAACCA AGAAACCATC ACTGCCGAGC AGGTGAAGAA GGTCCTCTTC 
GACGAGGAGG AGAGGAGCTC CGATTCCTCC CAACAGTGCT CATCGGAGGA TGAAGACATC 
TTTGAGGAGA CGGCGCAGGT GAGCCCTCCG CGAGGCAAGG AGAAAAGGCA ATGGAGGGCA 
CGCCTGCCGT CGCTCAGCGT GCAGCCGGTG AGCAATGCAG ACTGGGTGTG GCTGGTCAAG 
AGGCTACACA AGCTGTGCAT GGAGCTCTGT AACCACTACA TCCAGATGCA CCTGGACCTG 
GAGAGCAGCT TAGAGGAGCC TCCCGCCTTC AAAAGCGACC CGTTCTTCAT CCTGCCATCC 
TTCCAGTCTG AGTCGTCCAC ACCATCCACG GGTGGCTTCT CCGGGAAAAA CACGCCCTCG 
GAAGATGACC GCAGAGAGCA TCTAAGTGAG CCCCTAAGTC TGAGGGTGGG CAGTGGGGAC 
GTGCTGATGC TGCCCCCCAG TCCCAAGACA GAGAAGAAGG ATCCTGGCCG CAAGAAAGAG 
TGGTGGGAGA GCGCGGGGAA CAAGATTTGC ACCATGGCTG CCGACAAAAC CATCTCAAAG 
CTGATGACCG AGTACAAGAA GAGGAGGCAG CCGCACAACC TGCCTGCCTT CCCCAAGGAA 
GTCAAGGCAG ATAAGAAGGG CGAGCCCCTG GGGCCTCGGG GTCCTGATTC CCCGCTGCTT 
CAGCGGCCAC AGCATCTCAT GGACCAAGGA CAGATGCGCC ATTCCTTCAG TGCAGGCCCA 
GAGCTGTTGC GGCAGGAGAA GAGACCCCGC TCAGGCTCCA CCGGGAGCTC CCTGAGTGTC 
TCGGTGCGAG ACGCAGAGGC ACAGATCCAG GCGTGGACCA ACATGGTGCT AACAGTTCTC 
AATCAGATTC AAATCCTTCC AGACCAGACA TTCACGGCCC TCCAGCCAGC AGTGTTCCCG 
TGCATCAGCC AGCTGACCTG TCATGTGACT GACATCAGAG TCCGTCAAGC TGTAAGGGAA 
TGGCTGGGAA GAGTGGGCCG TGTCTATGAT ATCATCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Arfgef3 cDNA ORF clone, Mesocricetus auratus(golden hamster)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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