PLCE1 cDNA ORF clone, Mesocricetus auratus(Golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCE1
Entrez Gene ID	101832834
Full Name	phospholipase C epsilon 1
Gene Type	protein-coding
Organism	Mesocricetus auratus(Golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013116344.1	XP_012971798.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
XM_005063631.2	XP_005063688.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_005063631.3	XP_005063688.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
XM_013116335.1	XP_012971789.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following PLCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe117142	XM_013116335.1 Latest version!	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe117143	XM_013116344.1 Latest version!	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe117144	XM_005063631.3 Latest version!	Mesocricetus auratus phospholipase C epsilon 1 (Plce1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMe117144	XM_005063631.2	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCE1 ( XM_013116335.1 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012971789.1 Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.

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CloneID	OMe117142
Clone ID Related Accession (Same CDS sequence)	XM_013116335.1
Accession Version	XM_013116335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801604.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGGCCA GTCTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGTCCCT CCAGTGCCTG GAGCAGCAGC AGCTGGCATG GGCGGATCAA AGGAGGGATG 
AAGGGCTTCC AGAGCTTCAT GGTTTCGGAC AGTAACATGA GCTTCATTGA GTTTGTCGAG 
CTGTTCAAGT CGTTCAGCGT AAGGAGCCGC AAGGACCTGA AGGACATCTT CGATATCTAC 
TCTGTGCCCT GCAATCGGTC TGCCTCTGAG TCAGCCCCCC TGTACACCAA CCTGACCATT 
GAAGAAAACG CCAGTGACTT TCAGCCAGAC CTAGATTTGC TAACCAGAAA CGTCTCAGAC 
TTGGGGCTGT TCATTAAGAG CAAACAGCAA CTTTCTGACA ACCAGAGGCA GATATCGGAT 
GCTATTGCAG CGGCGAGCAT TGTGACTAAT GGCACTGGGA TTGAGAGCAC ATCCCTGGGC 
ATATTCGGGG TCGGCATACT CCAGCTCAAT GACTTCCTGG TGAATTGCCA AGGAGAACAC 
TGCACGTATG ATGAAATTCT CAGCATCATC CAGAAATTCG AGCCTAGCGT CAGTATGTGT 
CATCAGGGCC TAATGTCGTT TGAAGGGTTT GCAAGGTTTC TGATGGATAA AGATAATTTC 
GCCTCCAAAA ATGATGAGTC ACAAGAGAAC AAGAGAGAAC TGCAGCTACC CCTCTCCTAC 
TACTATATTG AATCTTCACA CAATACCTAC CTCACAGGCC ATCAGCTCAA GGGAGAATCC 
TCCGTGGAGC TGTACAGCCA GGTCCTCTTG CAAGGATGCA GGAGCATAGA GCTGGACTGC 
TGGGATGGAG ATGATGGCAT GCCCATTATT TACCATGGAC ATACACTGAC GACCAAGATC 
CCCTTCAAGG AAGTGGTGGA AGCCATTGAT CGCAGTGCCT TCATCACCTC TGACTTGCCA 
ATCATCATAT CCATTGAGAA CCACTGTTCA TTGCCTCAAC AACGAAAAAT GGCAGAAATT 
TTCAAGAGTG TGTTTGGAGA AAAGCTGGTG GCTAAATTCT TATTCGAGAC AGATTTCTCA 
GATGATCCAA TGCTTCCCTC ACCTGACCAA CTCAGGAAGA AAGTGCTTCT TAAAAACAAG 
AAGCTAAAGG CCCATCAGAC ACCAGTCGAC ATCCTAAAAC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG CACAGGCTTT CACTGGTGGG AGTGCCAACC CCCCACCTGC CAGTAATGAG 
GAAGAGGAAG ACGAAGAGGA TGAATATGAT TATGATTATG AATCCCTTTC TGATGACAAT 
ATTCTGGAAG ACAAACCTGA AAACAAATCA TGTGCTGACA AGCTTCAGTT TGAGTACAAT 
GAAGAAATCC CCAAGAGGAT AAAGAAAGCA GATAACTCTT CTTGCAATAA GGGAAAGGTA 
TATGACATGG AACTGGGGGA GGAGTTTTAC CTCCCTCAGA ACAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTCTCTGA CCTCATCATC TACTGCCAGG CAGTAAAATT TCCAGGCCTG 
TCGACTCTAA ATTCATCTGG CTCTAGCAGA GGGAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCTGGCAG AATGAGCCCA GGGGAGACAG CATCCTTCAA CAGAACATCT 
GGAAAAAGTT CCAACGAAGG AATTCGGCAA ACCTGGGAGG AATCGTGTTC GCCACTCAGC 
CCAAGCACAT CCCTCAGCGC TATCATTAGA ACTCCCAAAT GCTATCATAT CTCCTCACTG 
AATGAAAACG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTAAT CCAGCACACC 
GCTTGTCAGC TGCTGAGGAC CTACCCGGCA GCGACCCGAA TCGACTCATC CAATCCCAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCCC TCAACTACCA GACGGATGAT 
CTTCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGCTGTGG TTATGTTTTG 
AAGCCCCCTG TCCTGTGGGA TAAAAGCTGC CCCATGTACC AGAAGTTTTC TCCACTGGAA 
AGAGACCTAG ACAGCATGGA TCCTGCTATC TATTCTCTAA CTATCATCTC TGGCCAAAAT 
GTGTGCCCCA GTAACAGCAC CGGAAGTCCA TGCATCGAGG TTGATGTCTT GGGCATGCCC 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATAGGA ACCCCCTGAA CCCCATGTGG 
AACGAACAGT TTCTCTTCCG GGTTCACTTT GAAGATCTTG TATTTCTTCG TTTTGCCGTT 
GTGGAAAACA ACAGTTCGGC AATAACGGCT CAGAGAATCA TTCCACTCAA AGCTTTAAAA 
CGAGGTTATC GACATCTTCA GCTGCGGAAC CTCCACAATG AAATATTGGA AATCTCTAGC 
CTATTCATAA ATAGCAGAAG GATGGAAGAA AATCCCTCTG GCAGTACCAT GCCAGCCTCT 
TTGATGTTTA ATACTGAAGA GAGGAAATGT TCTCAGACTC ACAGAGTCAC AGTGCATGGT 
GTTCCAGGTC CGGAGCCCTT TGCTGTTTTC ACCATAAACG GAGGCACCAA GGCAAAGCAG 
CTTCTCCAAC AGGTTCTGGT GATTGACCCA GACACCAAAC TTAGTGCCAC AGACTACTTT 
CTGATGGAAG AGAAGTATTT TATATCTAAG GAAAAGAATG AGTGCAAGAA ACAACCATTC 
CAGAGAGCTG TGGGTCCAGA AGAAGATATT GTGCAGATTT TAAACAGCTG GTTTCCAGAA 
GAAGGCTATG TAGGCAGGAT TGTCTTAAAG CCCCAGCAGG AAACCCTAGA AGAGAAAAGC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCGGAGG AGGAGAGTTT CTTTGTCCAA 
GTTCATGATG TCTCTCCAGA GCAACCCCGA ACTGTCATCA AAGCCCCTCG GGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG TATCCTGAAC 
AACCCCAACC CAGGTGACTA TGTGCTTTTG GAAGAGGTGA TGAAAGAAGC ACCCAGTAAG 
AAGTCTTCTA CTCCAAAGTC CTCACAGAGG ATCCTTTTGG ACCAAGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGGAAA TTCATCCTGA AGCTAAAGGA GCAGGTTCAG 
GCATCCCGAG AAGATAAAAG GAAGGGCATC TCCTTTGCAA GTGAACTCAA GAAACTCACT 
AAGTCCACCA AGCAGCCCCG AGGACTCGCA TCACCTCCTC AGCTTGTGGC CCCAGAAAGT 
GTCCAAATCA AGGAGGAGAA ACCTGTAGGT GCCCTGTCCT CCAGTGACAC AGTGGCTTAC 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971789.1
CDS	502..7410
Translation	MTSEEMAASILIPVTQRKVASAQSAVAEDKSENVLDAHIPKTCAGRHSGQIPHTISQWNKPEAGPSRSDLSQLFPIASGEVMSDENHNEKCWEKNLPDSVKNHAINCNSLLQSHQSDRPQSQLCVACDSVSGEDLCLQTGISSPLERKVFPGIQLEMDDSPMNVSPLGNEPGLTGTRGPHPDSNMAVFHFRYEADRTISDAFHTLSEKLILDDCANCVTLPGGQQKKTCMAYTCKLVELTKTCGRKNGQLQCEPCSSLGDEHLCFESSCQQADDVLCSSRMFFREGFAHNPPAKTFLSPLEDFSDNCEDGDEFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSGHIGPAAWKHVEQRRARLIGPCGDVMEPLSAIDVRPRGTQHLTEAQWCLIYSAVRRGEETEDTIGSLLHCSRQLPTSETACGRIRDGPCLKQCVRDTECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSNFGGSTGRIMLKERQLGTSMANSNTVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRKVLPMDYLCFLTRDLSSPECQSSLPHLKASISASILTSQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSLEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEAALEFVADYSGQDNFLQRVGQNGLKNTEKESTVNSIFQIIRSCSRSLEMEEEDSSSEGAGSRKNSLKDKTRWQLIIGDFLDSENDIFEKSKECDLHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATAIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTSSPAGARPKFGVLSNMSEPGKFPSVGSPGLSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSLLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMIFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNVSPGMSTKNAEKSSIQRNNTLGISTTKKKKKMLMRGESGEATDDETATRKAKMCRECRSRSGSDPQDANEQEESEANVITNPPNPLHSRRAHSLTTAGSSNLTTGMSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSVRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENASDFQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSVSMCHQGLMSFEGFARFLMDKDNFASKNDESQENKRELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGSANPPPASNEEEEDEEDEYDYDYESLSDDNILEDKPENKSCADKLQFEYNEEIPKRIKKADNSSCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLIIYCQAVKFPGLSTLNSSGSSRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSNEGIRQTWEESCSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDSMDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNPLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEENPSGSTMPASLMFNTEERKCSQTHRVTVHGVPGPEPFAVFTINGGTKAKQLLQQVLVIDPDTKLSATDYFLMEEKYFISKEKNECKKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKSIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPGDYVLLEEVMKEAPSKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRKGISFASELKKLTKSTKQPRGLASPPQLVAPESVQIKEEKPVGALSSSDTVAYQQ

Target ORF information:

RefSeq Version	XM_013116335.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116335.1

ORF Insert Sequence:

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGGCCA GTCTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGTCCCT CCAGTGCCTG GAGCAGCAGC AGCTGGCATG GGCGGATCAA AGGAGGGATG 
AAGGGCTTCC AGAGCTTCAT GGTTTCGGAC AGTAACATGA GCTTCATTGA GTTTGTCGAG 
CTGTTCAAGT CGTTCAGCGT AAGGAGCCGC AAGGACCTGA AGGACATCTT CGATATCTAC 
TCTGTGCCCT GCAATCGGTC TGCCTCTGAG TCAGCCCCCC TGTACACCAA CCTGACCATT 
GAAGAAAACG CCAGTGACTT TCAGCCAGAC CTAGATTTGC TAACCAGAAA CGTCTCAGAC 
TTGGGGCTGT TCATTAAGAG CAAACAGCAA CTTTCTGACA ACCAGAGGCA GATATCGGAT 
GCTATTGCAG CGGCGAGCAT TGTGACTAAT GGCACTGGGA TTGAGAGCAC ATCCCTGGGC 
ATATTCGGGG TCGGCATACT CCAGCTCAAT GACTTCCTGG TGAATTGCCA AGGAGAACAC 
TGCACGTATG ATGAAATTCT CAGCATCATC CAGAAATTCG AGCCTAGCGT CAGTATGTGT 
CATCAGGGCC TAATGTCGTT TGAAGGGTTT GCAAGGTTTC TGATGGATAA AGATAATTTC 
GCCTCCAAAA ATGATGAGTC ACAAGAGAAC AAGAGAGAAC TGCAGCTACC CCTCTCCTAC 
TACTATATTG AATCTTCACA CAATACCTAC CTCACAGGCC ATCAGCTCAA GGGAGAATCC 
TCCGTGGAGC TGTACAGCCA GGTCCTCTTG CAAGGATGCA GGAGCATAGA GCTGGACTGC 
TGGGATGGAG ATGATGGCAT GCCCATTATT TACCATGGAC ATACACTGAC GACCAAGATC 
CCCTTCAAGG AAGTGGTGGA AGCCATTGAT CGCAGTGCCT TCATCACCTC TGACTTGCCA 
ATCATCATAT CCATTGAGAA CCACTGTTCA TTGCCTCAAC AACGAAAAAT GGCAGAAATT 
TTCAAGAGTG TGTTTGGAGA AAAGCTGGTG GCTAAATTCT TATTCGAGAC AGATTTCTCA 
GATGATCCAA TGCTTCCCTC ACCTGACCAA CTCAGGAAGA AAGTGCTTCT TAAAAACAAG 
AAGCTAAAGG CCCATCAGAC ACCAGTCGAC ATCCTAAAAC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG CACAGGCTTT CACTGGTGGG AGTGCCAACC CCCCACCTGC CAGTAATGAG 
GAAGAGGAAG ACGAAGAGGA TGAATATGAT TATGATTATG AATCCCTTTC TGATGACAAT 
ATTCTGGAAG ACAAACCTGA AAACAAATCA TGTGCTGACA AGCTTCAGTT TGAGTACAAT 
GAAGAAATCC CCAAGAGGAT AAAGAAAGCA GATAACTCTT CTTGCAATAA GGGAAAGGTA 
TATGACATGG AACTGGGGGA GGAGTTTTAC CTCCCTCAGA ACAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTCTCTGA CCTCATCATC TACTGCCAGG CAGTAAAATT TCCAGGCCTG 
TCGACTCTAA ATTCATCTGG CTCTAGCAGA GGGAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCTGGCAG AATGAGCCCA GGGGAGACAG CATCCTTCAA CAGAACATCT 
GGAAAAAGTT CCAACGAAGG AATTCGGCAA ACCTGGGAGG AATCGTGTTC GCCACTCAGC 
CCAAGCACAT CCCTCAGCGC TATCATTAGA ACTCCCAAAT GCTATCATAT CTCCTCACTG 
AATGAAAACG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTAAT CCAGCACACC 
GCTTGTCAGC TGCTGAGGAC CTACCCGGCA GCGACCCGAA TCGACTCATC CAATCCCAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCCC TCAACTACCA GACGGATGAT 
CTTCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGCTGTGG TTATGTTTTG 
AAGCCCCCTG TCCTGTGGGA TAAAAGCTGC CCCATGTACC AGAAGTTTTC TCCACTGGAA 
AGAGACCTAG ACAGCATGGA TCCTGCTATC TATTCTCTAA CTATCATCTC TGGCCAAAAT 
GTGTGCCCCA GTAACAGCAC CGGAAGTCCA TGCATCGAGG TTGATGTCTT GGGCATGCCC 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATAGGA ACCCCCTGAA CCCCATGTGG 
AACGAACAGT TTCTCTTCCG GGTTCACTTT GAAGATCTTG TATTTCTTCG TTTTGCCGTT 
GTGGAAAACA ACAGTTCGGC AATAACGGCT CAGAGAATCA TTCCACTCAA AGCTTTAAAA 
CGAGGTTATC GACATCTTCA GCTGCGGAAC CTCCACAATG AAATATTGGA AATCTCTAGC 
CTATTCATAA ATAGCAGAAG GATGGAAGAA AATCCCTCTG GCAGTACCAT GCCAGCCTCT 
TTGATGTTTA ATACTGAAGA GAGGAAATGT TCTCAGACTC ACAGAGTCAC AGTGCATGGT 
GTTCCAGGTC CGGAGCCCTT TGCTGTTTTC ACCATAAACG GAGGCACCAA GGCAAAGCAG 
CTTCTCCAAC AGGTTCTGGT GATTGACCCA GACACCAAAC TTAGTGCCAC AGACTACTTT 
CTGATGGAAG AGAAGTATTT TATATCTAAG GAAAAGAATG AGTGCAAGAA ACAACCATTC 
CAGAGAGCTG TGGGTCCAGA AGAAGATATT GTGCAGATTT TAAACAGCTG GTTTCCAGAA 
GAAGGCTATG TAGGCAGGAT TGTCTTAAAG CCCCAGCAGG AAACCCTAGA AGAGAAAAGC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCGGAGG AGGAGAGTTT CTTTGTCCAA 
GTTCATGATG TCTCTCCAGA GCAACCCCGA ACTGTCATCA AAGCCCCTCG GGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG TATCCTGAAC 
AACCCCAACC CAGGTGACTA TGTGCTTTTG GAAGAGGTGA TGAAAGAAGC ACCCAGTAAG 
AAGTCTTCTA CTCCAAAGTC CTCACAGAGG ATCCTTTTGG ACCAAGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGGAAA TTCATCCTGA AGCTAAAGGA GCAGGTTCAG 
GCATCCCGAG AAGATAAAAG GAAGGGCATC TCCTTTGCAA GTGAACTCAA GAAACTCACT 
AAGTCCACCA AGCAGCCCCG AGGACTCGCA TCACCTCCTC AGCTTGTGGC CCCAGAAAGT 
GTCCAAATCA AGGAGGAGAA ACCTGTAGGT GCCCTGTCCT CCAGTGACAC AGTGGCTTAC 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_013116344.1 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012971798.1 Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.

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CloneID	OMe117143
Clone ID Related Accession (Same CDS sequence)	XM_013116344.1
Accession Version	XM_013116344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801604.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGCAG ATGTGCTTAC TGCTTCCCCT CTTCCTAACA GTCAGGAAGA CAATATTCTG 
GAAGACAAAC CTGAAAACAA ATCATGTGCT GACAAGCTTC AGTTTGAGTA CAATGAAGAA 
ATCCCCAAGA GGATAAAGAA AGCAGATAAC TCTTCTTGCA ATAAGGGAAA GGTATATGAC 
ATGGAACTGG GGGAGGAGTT TTACCTCCCT CAGAACAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTCT CTGACCTCAT CATCTACTGC CAGGCAGTAA AATTTCCAGG CCTGTCGACT 
CTAAATTCAT CTGGCTCTAG CAGAGGGAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCTG GCAGAATGAG CCCAGGGGAG ACAGCATCCT TCAACAGAAC ATCTGGAAAA 
AGTTCCAACG AAGGAATTCG GCAAACCTGG GAGGAATCGT GTTCGCCACT CAGCCCAAGC 
ACATCCCTCA GCGCTATCAT TAGAACTCCC AAATGCTATC ATATCTCCTC ACTGAATGAA 
AACGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TAATCCAGCA CACCGCTTGT 
CAGCTGCTGA GGACCTACCC GGCAGCGACC CGAATCGACT CATCCAATCC CAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCCCTCAACT ACCAGACGGA TGATCTTCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGCT GTGGTTATGT TTTGAAGCCC 
CCTGTCCTGT GGGATAAAAG CTGCCCCATG TACCAGAAGT TTTCTCCACT GGAAAGAGAC 
CTAGACAGCA TGGATCCTGC TATCTATTCT CTAACTATCA TCTCTGGCCA AAATGTGTGC 
CCCAGTAACA GCACCGGAAG TCCATGCATC GAGGTTGATG TCTTGGGCAT GCCCCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT AGGAACCCCC TGAACCCCAT GTGGAACGAA 
CAGTTTCTCT TCCGGGTTCA CTTTGAAGAT CTTGTATTTC TTCGTTTTGC CGTTGTGGAA 
AACAACAGTT CGGCAATAAC GGCTCAGAGA ATCATTCCAC TCAAAGCTTT AAAACGAGGT 
TATCGACATC TTCAGCTGCG GAACCTCCAC AATGAAATAT TGGAAATCTC TAGCCTATTC 
ATAAATAGCA GAAGGATGGA AGAAAATCCC TCTGGCAGTA CCATGCCAGC CTCTTTGATG 
TTTAATACTG AAGAGAGGAA ATGTTCTCAG ACTCACAGAG TCACAGTGCA TGGTGTTCCA 
GGTCCGGAGC CCTTTGCTGT TTTCACCATA AACGGAGGCA CCAAGGCAAA GCAGCTTCTC 
CAACAGGTTC TGGTGATTGA CCCAGACACC AAACTTAGTG CCACAGACTA CTTTCTGATG 
GAAGAGAAGT ATTTTATATC TAAGGAAAAG AATGAGTGCA AGAAACAACC ATTCCAGAGA 
GCTGTGGGTC CAGAAGAAGA TATTGTGCAG ATTTTAAACA GCTGGTTTCC AGAAGAAGGC 
TATGTAGGCA GGATTGTCTT AAAGCCCCAG CAGGAAACCC TAGAAGAGAA AAGCATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCG GAGGAGGAGA GTTTCTTTGT CCAAGTTCAT 
GATGTCTCTC CAGAGCAACC CCGAACTGTC ATCAAAGCCC CTCGGGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGTATCCT GAACAACCCC 
AACCCAGGTG ACTATGTGCT TTTGGAAGAG GTGATGAAAG AAGCACCCAG TAAGAAGTCT 
TCTACTCCAA AGTCCTCACA GAGGATCCTT TTGGACCAAG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG GAAATTCATC CTGAAGCTAA AGGAGCAGGT TCAGGCATCC 
CGAGAAGATA AAAGGAAGGG CATCTCCTTT GCAAGTGAAC TCAAGAAACT CACTAAGTCC 
ACCAAGCAGC CCCGAGGACT CGCATCACCT CCTCAGCTTG TGGCCCCAGA AAGTGTCCAA 
ATCAAGGAGG AGAAACCTGT AGGTGCCCTG TCCTCCAGTG ACACAGTGGC TTACCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971798.1
CDS	502..7404
Translation	MTSEEMAASILIPVTQRKVASAQSAVAEDKSENVLDAHIPKTCAGRHSGQIPHTISQWNKPEAGPSRSDLSQLFPIASGEVMSDENHNEKCWEKNLPDSVKNHAINCNSLLQSHQSDRPQSQLCVACDSVSGEDLCLQTGISSPLERKVFPGIQLEMDDSPMNVSPLGNEPGLTGTRGPHPDSNMAVFHFRYEADRTISDAFHTLSEKLILDDCANCVTLPGGQQKKTCMAYTCKLVELTKTCGRKNGQLQCEPCSSLGDEHLCFESSCQQADDVLCSSRMFFREGFAHNPPAKTFLSPLEDFSDNCEDGDEFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSGHIGPAAWKHVEQRRARLIGPCGDVMEPLSAIDVRPRGTQHLTEAQWCLIYSAVRRGEETEDTIGSLLHCSRQLPTSETACGRIRDGPCLKQCVRDTECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSNFGGSTGRIMLKERQLGTSMANSNTVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRKVLPMDYLCFLTRDLSSPECQSSLPHLKASISASILTSQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSLEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEAALEFVADYSGQDNFLQRVGQNGLKNTEKESTVNSIFQIIRSCSRSLEMEEEDSSSEGAGSRKNSLKDKTRWQLIIGDFLDSENDIFEKSKECDLHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATAIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTSSPAGARPKFGVLSNMSEPGKFPSVGSPGLSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSLLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMIFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNVSPGMSTKNAEKSSIQRNNTLGISTTKKKKKMLMRGESGEATDDETATRKAKMCRECRSRSGSDPQDANEQEESEANVITNPPNPLHSRRAHSLTTAGSSNLTTGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSVRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENASDFQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSVSMCHQGLMSFEGFARFLMDKDNFASKNDESQENKRELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGSANPPPASNEEEEDEEDEYDYDYESLSDADVLTASPLPNSQEDNILEDKPENKSCADKLQFEYNEEIPKRIKKADNSSCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLIIYCQAVKFPGLSTLNSSGSSRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSNEGIRQTWEESCSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDSMDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNPLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEENPSGSTMPASLMFNTEERKCSQTHRVTVHGVPGPEPFAVFTINGGTKAKQLLQQVLVIDPDTKLSATDYFLMEEKYFISKEKNECKKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKSIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPGDYVLLEEVMKEAPSKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRKGISFASELKKLTKSTKQPRGLASPPQLVAPESVQIKEEKPVGALSSSDTVAYQQ

Target ORF information:

RefSeq Version	XM_013116344.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116344.1

ORF Insert Sequence:

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGCAG ATGTGCTTAC TGCTTCCCCT CTTCCTAACA GTCAGGAAGA CAATATTCTG 
GAAGACAAAC CTGAAAACAA ATCATGTGCT GACAAGCTTC AGTTTGAGTA CAATGAAGAA 
ATCCCCAAGA GGATAAAGAA AGCAGATAAC TCTTCTTGCA ATAAGGGAAA GGTATATGAC 
ATGGAACTGG GGGAGGAGTT TTACCTCCCT CAGAACAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTCT CTGACCTCAT CATCTACTGC CAGGCAGTAA AATTTCCAGG CCTGTCGACT 
CTAAATTCAT CTGGCTCTAG CAGAGGGAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCTG GCAGAATGAG CCCAGGGGAG ACAGCATCCT TCAACAGAAC ATCTGGAAAA 
AGTTCCAACG AAGGAATTCG GCAAACCTGG GAGGAATCGT GTTCGCCACT CAGCCCAAGC 
ACATCCCTCA GCGCTATCAT TAGAACTCCC AAATGCTATC ATATCTCCTC ACTGAATGAA 
AACGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TAATCCAGCA CACCGCTTGT 
CAGCTGCTGA GGACCTACCC GGCAGCGACC CGAATCGACT CATCCAATCC CAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCCCTCAACT ACCAGACGGA TGATCTTCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGCT GTGGTTATGT TTTGAAGCCC 
CCTGTCCTGT GGGATAAAAG CTGCCCCATG TACCAGAAGT TTTCTCCACT GGAAAGAGAC 
CTAGACAGCA TGGATCCTGC TATCTATTCT CTAACTATCA TCTCTGGCCA AAATGTGTGC 
CCCAGTAACA GCACCGGAAG TCCATGCATC GAGGTTGATG TCTTGGGCAT GCCCCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT AGGAACCCCC TGAACCCCAT GTGGAACGAA 
CAGTTTCTCT TCCGGGTTCA CTTTGAAGAT CTTGTATTTC TTCGTTTTGC CGTTGTGGAA 
AACAACAGTT CGGCAATAAC GGCTCAGAGA ATCATTCCAC TCAAAGCTTT AAAACGAGGT 
TATCGACATC TTCAGCTGCG GAACCTCCAC AATGAAATAT TGGAAATCTC TAGCCTATTC 
ATAAATAGCA GAAGGATGGA AGAAAATCCC TCTGGCAGTA CCATGCCAGC CTCTTTGATG 
TTTAATACTG AAGAGAGGAA ATGTTCTCAG ACTCACAGAG TCACAGTGCA TGGTGTTCCA 
GGTCCGGAGC CCTTTGCTGT TTTCACCATA AACGGAGGCA CCAAGGCAAA GCAGCTTCTC 
CAACAGGTTC TGGTGATTGA CCCAGACACC AAACTTAGTG CCACAGACTA CTTTCTGATG 
GAAGAGAAGT ATTTTATATC TAAGGAAAAG AATGAGTGCA AGAAACAACC ATTCCAGAGA 
GCTGTGGGTC CAGAAGAAGA TATTGTGCAG ATTTTAAACA GCTGGTTTCC AGAAGAAGGC 
TATGTAGGCA GGATTGTCTT AAAGCCCCAG CAGGAAACCC TAGAAGAGAA AAGCATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCG GAGGAGGAGA GTTTCTTTGT CCAAGTTCAT 
GATGTCTCTC CAGAGCAACC CCGAACTGTC ATCAAAGCCC CTCGGGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGTATCCT GAACAACCCC 
AACCCAGGTG ACTATGTGCT TTTGGAAGAG GTGATGAAAG AAGCACCCAG TAAGAAGTCT 
TCTACTCCAA AGTCCTCACA GAGGATCCTT TTGGACCAAG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG GAAATTCATC CTGAAGCTAA AGGAGCAGGT TCAGGCATCC 
CGAGAAGATA AAAGGAAGGG CATCTCCTTT GCAAGTGAAC TCAAGAAACT CACTAAGTCC 
ACCAAGCAGC CCCGAGGACT CGCATCACCT CCTCAGCTTG TGGCCCCAGA AAGTGTCCAA 
ATCAAGGAGG AGAAACCTGT AGGTGCCCTG TCCTCCAGTG ACACAGTGGC TTACCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_005063631.2 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_005063688.1 Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.

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CloneID	OMe117144
Clone ID Related Accession (Same CDS sequence)	XM_005063631.2 , XM_005063631.3
Accession Version	XM_005063631.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801604.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005063631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGACA ATATTCTGGA AGACAAACCT GAAAACAAAT CATGTGCTGA CAAGCTTCAG 
TTTGAGTACA ATGAAGAAAT CCCCAAGAGG ATAAAGAAAG CAGATAACTC TTCTTGCAAT 
AAGGGAAAGG TATATGACAT GGAACTGGGG GAGGAGTTTT ACCTCCCTCA GAACAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTCTCT GACCTCATCA TCTACTGCCA GGCAGTAAAA 
TTTCCAGGCC TGTCGACTCT AAATTCATCT GGCTCTAGCA GAGGGAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCTGGC AGAATGAGCC CAGGGGAGAC AGCATCCTTC 
AACAGAACAT CTGGAAAAAG TTCCAACGAA GGAATTCGGC AAACCTGGGA GGAATCGTGT 
TCGCCACTCA GCCCAAGCAC ATCCCTCAGC GCTATCATTA GAACTCCCAA ATGCTATCAT 
ATCTCCTCAC TGAATGAAAA CGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTA 
ATCCAGCACA CCGCTTGTCA GCTGCTGAGG ACCTACCCGG CAGCGACCCG AATCGACTCA 
TCCAATCCCA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC CCTCAACTAC 
CAGACGGATG ATCTTCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGCTGT 
GGTTATGTTT TGAAGCCCCC TGTCCTGTGG GATAAAAGCT GCCCCATGTA CCAGAAGTTT 
TCTCCACTGG AAAGAGACCT AGACAGCATG GATCCTGCTA TCTATTCTCT AACTATCATC 
TCTGGCCAAA ATGTGTGCCC CAGTAACAGC ACCGGAAGTC CATGCATCGA GGTTGATGTC 
TTGGGCATGC CCCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATAG GAACCCCCTG 
AACCCCATGT GGAACGAACA GTTTCTCTTC CGGGTTCACT TTGAAGATCT TGTATTTCTT 
CGTTTTGCCG TTGTGGAAAA CAACAGTTCG GCAATAACGG CTCAGAGAAT CATTCCACTC 
AAAGCTTTAA AACGAGGTTA TCGACATCTT CAGCTGCGGA ACCTCCACAA TGAAATATTG 
GAAATCTCTA GCCTATTCAT AAATAGCAGA AGGATGGAAG AAAATCCCTC TGGCAGTACC 
ATGCCAGCCT CTTTGATGTT TAATACTGAA GAGAGGAAAT GTTCTCAGAC TCACAGAGTC 
ACAGTGCATG GTGTTCCAGG TCCGGAGCCC TTTGCTGTTT TCACCATAAA CGGAGGCACC 
AAGGCAAAGC AGCTTCTCCA ACAGGTTCTG GTGATTGACC CAGACACCAA ACTTAGTGCC 
ACAGACTACT TTCTGATGGA AGAGAAGTAT TTTATATCTA AGGAAAAGAA TGAGTGCAAG 
AAACAACCAT TCCAGAGAGC TGTGGGTCCA GAAGAAGATA TTGTGCAGAT TTTAAACAGC 
TGGTTTCCAG AAGAAGGCTA TGTAGGCAGG ATTGTCTTAA AGCCCCAGCA GGAAACCCTA 
GAAGAGAAAA GCATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCGGA GGAGGAGAGT 
TTCTTTGTCC AAGTTCATGA TGTCTCTCCA GAGCAACCCC GAACTGTCAT CAAAGCCCCT 
CGGGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGTATCCTGA ACAACCCCAA CCCAGGTGAC TATGTGCTTT TGGAAGAGGT GATGAAAGAA 
GCACCCAGTA AGAAGTCTTC TACTCCAAAG TCCTCACAGA GGATCCTTTT GGACCAAGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGGA AATTCATCCT GAAGCTAAAG 
GAGCAGGTTC AGGCATCCCG AGAAGATAAA AGGAAGGGCA TCTCCTTTGC AAGTGAACTC 
AAGAAACTCA CTAAGTCCAC CAAGCAGCCC CGAGGACTCG CATCACCTCC TCAGCTTGTG 
GCCCCAGAAA GTGTCCAAAT CAAGGAGGAG AAACCTGTAG GTGCCCTGTC CTCCAGTGAC 
ACAGTGGCTT ACCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005063688.1
CDS	502..7362
Translation	MTSEEMAASILIPVTQRKVASAQSAVAEDKSENVLDAHIPKTCAGRHSGQIPHTISQWNKPEAGPSRSDLSQLFPIASGEVMSDENHNEKCWEKNLPDSVKNHAINCNSLLQSHQSDRPQSQLCVACDSVSGEDLCLQTGISSPLERKVFPGIQLEMDDSPMNVSPLGNEPGLTGTRGPHPDSNMAVFHFRYEADRTISDAFHTLSEKLILDDCANCVTLPGGQQKKTCMAYTCKLVELTKTCGRKNGQLQCEPCSSLGDEHLCFESSCQQADDVLCSSRMFFREGFAHNPPAKTFLSPLEDFSDNCEDGDEFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSGHIGPAAWKHVEQRRARLIGPCGDVMEPLSAIDVRPRGTQHLTEAQWCLIYSAVRRGEETEDTIGSLLHCSRQLPTSETACGRIRDGPCLKQCVRDTECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSNFGGSTGRIMLKERQLGTSMANSNTVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRKVLPMDYLCFLTRDLSSPECQSSLPHLKASISASILTSQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSLEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEAALEFVADYSGQDNFLQRVGQNGLKNTEKESTVNSIFQIIRSCSRSLEMEEEDSSSEGAGSRKNSLKDKTRWQLIIGDFLDSENDIFEKSKECDLHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATAIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTSSPAGARPKFGVLSNMSEPGKFPSVGSPGLSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSLLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMIFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNVSPGMSTKNAEKSSIQRNNTLGISTTKKKKKMLMRGESGEATDDETATRKAKMCRECRSRSGSDPQDANEQEESEANVITNPPNPLHSRRAHSLTTAGSSNLTTGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSVRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENASDFQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSVSMCHQGLMSFEGFARFLMDKDNFASKNDESQENKRELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGSANPPPASNEEEEDEEDEYDYDYESLSDDNILEDKPENKSCADKLQFEYNEEIPKRIKKADNSSCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLIIYCQAVKFPGLSTLNSSGSSRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSNEGIRQTWEESCSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDSMDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNPLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEENPSGSTMPASLMFNTEERKCSQTHRVTVHGVPGPEPFAVFTINGGTKAKQLLQQVLVIDPDTKLSATDYFLMEEKYFISKEKNECKKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKSIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPGDYVLLEEVMKEAPSKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRKGISFASELKKLTKSTKQPRGLASPPQLVAPESVQIKEEKPVGALSSSDTVAYQQ

Target ORF information:

RefSeq Version	XM_005063631.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005063631.2

ORF Insert Sequence:

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGACA ATATTCTGGA AGACAAACCT GAAAACAAAT CATGTGCTGA CAAGCTTCAG 
TTTGAGTACA ATGAAGAAAT CCCCAAGAGG ATAAAGAAAG CAGATAACTC TTCTTGCAAT 
AAGGGAAAGG TATATGACAT GGAACTGGGG GAGGAGTTTT ACCTCCCTCA GAACAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTCTCT GACCTCATCA TCTACTGCCA GGCAGTAAAA 
TTTCCAGGCC TGTCGACTCT AAATTCATCT GGCTCTAGCA GAGGGAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCTGGC AGAATGAGCC CAGGGGAGAC AGCATCCTTC 
AACAGAACAT CTGGAAAAAG TTCCAACGAA GGAATTCGGC AAACCTGGGA GGAATCGTGT 
TCGCCACTCA GCCCAAGCAC ATCCCTCAGC GCTATCATTA GAACTCCCAA ATGCTATCAT 
ATCTCCTCAC TGAATGAAAA CGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTA 
ATCCAGCACA CCGCTTGTCA GCTGCTGAGG ACCTACCCGG CAGCGACCCG AATCGACTCA 
TCCAATCCCA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC CCTCAACTAC 
CAGACGGATG ATCTTCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGCTGT 
GGTTATGTTT TGAAGCCCCC TGTCCTGTGG GATAAAAGCT GCCCCATGTA CCAGAAGTTT 
TCTCCACTGG AAAGAGACCT AGACAGCATG GATCCTGCTA TCTATTCTCT AACTATCATC 
TCTGGCCAAA ATGTGTGCCC CAGTAACAGC ACCGGAAGTC CATGCATCGA GGTTGATGTC 
TTGGGCATGC CCCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATAG GAACCCCCTG 
AACCCCATGT GGAACGAACA GTTTCTCTTC CGGGTTCACT TTGAAGATCT TGTATTTCTT 
CGTTTTGCCG TTGTGGAAAA CAACAGTTCG GCAATAACGG CTCAGAGAAT CATTCCACTC 
AAAGCTTTAA AACGAGGTTA TCGACATCTT CAGCTGCGGA ACCTCCACAA TGAAATATTG 
GAAATCTCTA GCCTATTCAT AAATAGCAGA AGGATGGAAG AAAATCCCTC TGGCAGTACC 
ATGCCAGCCT CTTTGATGTT TAATACTGAA GAGAGGAAAT GTTCTCAGAC TCACAGAGTC 
ACAGTGCATG GTGTTCCAGG TCCGGAGCCC TTTGCTGTTT TCACCATAAA CGGAGGCACC 
AAGGCAAAGC AGCTTCTCCA ACAGGTTCTG GTGATTGACC CAGACACCAA ACTTAGTGCC 
ACAGACTACT TTCTGATGGA AGAGAAGTAT TTTATATCTA AGGAAAAGAA TGAGTGCAAG 
AAACAACCAT TCCAGAGAGC TGTGGGTCCA GAAGAAGATA TTGTGCAGAT TTTAAACAGC 
TGGTTTCCAG AAGAAGGCTA TGTAGGCAGG ATTGTCTTAA AGCCCCAGCA GGAAACCCTA 
GAAGAGAAAA GCATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCGGA GGAGGAGAGT 
TTCTTTGTCC AAGTTCATGA TGTCTCTCCA GAGCAACCCC GAACTGTCAT CAAAGCCCCT 
CGGGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGTATCCTGA ACAACCCCAA CCCAGGTGAC TATGTGCTTT TGGAAGAGGT GATGAAAGAA 
GCACCCAGTA AGAAGTCTTC TACTCCAAAG TCCTCACAGA GGATCCTTTT GGACCAAGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGGA AATTCATCCT GAAGCTAAAG 
GAGCAGGTTC AGGCATCCCG AGAAGATAAA AGGAAGGGCA TCTCCTTTGC AAGTGAACTC 
AAGAAACTCA CTAAGTCCAC CAAGCAGCCC CGAGGACTCG CATCACCTCC TCAGCTTGTG 
GCCCCAGAAA GTGTCCAAAT CAAGGAGGAG AAACCTGTAG GTGCCCTGTC CTCCAGTGAC 
ACAGTGGCTT ACCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_005063631.3 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_005063688.1 Mesocricetus auratus phospholipase C epsilon 1 (Plce1), mRNA.

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CloneID	OMe117144
Clone ID Related Accession (Same CDS sequence)	XM_005063631.2 , XM_005063631.3
Accession Version	XM_005063631.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801604.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005063631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGACA ATATTCTGGA AGACAAACCT GAAAACAAAT CATGTGCTGA CAAGCTTCAG 
TTTGAGTACA ATGAAGAAAT CCCCAAGAGG ATAAAGAAAG CAGATAACTC TTCTTGCAAT 
AAGGGAAAGG TATATGACAT GGAACTGGGG GAGGAGTTTT ACCTCCCTCA GAACAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTCTCT GACCTCATCA TCTACTGCCA GGCAGTAAAA 
TTTCCAGGCC TGTCGACTCT AAATTCATCT GGCTCTAGCA GAGGGAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCTGGC AGAATGAGCC CAGGGGAGAC AGCATCCTTC 
AACAGAACAT CTGGAAAAAG TTCCAACGAA GGAATTCGGC AAACCTGGGA GGAATCGTGT 
TCGCCACTCA GCCCAAGCAC ATCCCTCAGC GCTATCATTA GAACTCCCAA ATGCTATCAT 
ATCTCCTCAC TGAATGAAAA CGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTA 
ATCCAGCACA CCGCTTGTCA GCTGCTGAGG ACCTACCCGG CAGCGACCCG AATCGACTCA 
TCCAATCCCA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC CCTCAACTAC 
CAGACGGATG ATCTTCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGCTGT 
GGTTATGTTT TGAAGCCCCC TGTCCTGTGG GATAAAAGCT GCCCCATGTA CCAGAAGTTT 
TCTCCACTGG AAAGAGACCT AGACAGCATG GATCCTGCTA TCTATTCTCT AACTATCATC 
TCTGGCCAAA ATGTGTGCCC CAGTAACAGC ACCGGAAGTC CATGCATCGA GGTTGATGTC 
TTGGGCATGC CCCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATAG GAACCCCCTG 
AACCCCATGT GGAACGAACA GTTTCTCTTC CGGGTTCACT TTGAAGATCT TGTATTTCTT 
CGTTTTGCCG TTGTGGAAAA CAACAGTTCG GCAATAACGG CTCAGAGAAT CATTCCACTC 
AAAGCTTTAA AACGAGGTTA TCGACATCTT CAGCTGCGGA ACCTCCACAA TGAAATATTG 
GAAATCTCTA GCCTATTCAT AAATAGCAGA AGGATGGAAG AAAATCCCTC TGGCAGTACC 
ATGCCAGCCT CTTTGATGTT TAATACTGAA GAGAGGAAAT GTTCTCAGAC TCACAGAGTC 
ACAGTGCATG GTGTTCCAGG TCCGGAGCCC TTTGCTGTTT TCACCATAAA CGGAGGCACC 
AAGGCAAAGC AGCTTCTCCA ACAGGTTCTG GTGATTGACC CAGACACCAA ACTTAGTGCC 
ACAGACTACT TTCTGATGGA AGAGAAGTAT TTTATATCTA AGGAAAAGAA TGAGTGCAAG 
AAACAACCAT TCCAGAGAGC TGTGGGTCCA GAAGAAGATA TTGTGCAGAT TTTAAACAGC 
TGGTTTCCAG AAGAAGGCTA TGTAGGCAGG ATTGTCTTAA AGCCCCAGCA GGAAACCCTA 
GAAGAGAAAA GCATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCGGA GGAGGAGAGT 
TTCTTTGTCC AAGTTCATGA TGTCTCTCCA GAGCAACCCC GAACTGTCAT CAAAGCCCCT 
CGGGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGTATCCTGA ACAACCCCAA CCCAGGTGAC TATGTGCTTT TGGAAGAGGT GATGAAAGAA 
GCACCCAGTA AGAAGTCTTC TACTCCAAAG TCCTCACAGA GGATCCTTTT GGACCAAGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGGA AATTCATCCT GAAGCTAAAG 
GAGCAGGTTC AGGCATCCCG AGAAGATAAA AGGAAGGGCA TCTCCTTTGC AAGTGAACTC 
AAGAAACTCA CTAAGTCCAC CAAGCAGCCC CGAGGACTCG CATCACCTCC TCAGCTTGTG 
GCCCCAGAAA GTGTCCAAAT CAAGGAGGAG AAACCTGTAG GTGCCCTGTC CTCCAGTGAC 
ACAGTGGCTT ACCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005063688.1
CDS	509..7369
Translation	MTSEEMAASILIPVTQRKVASAQSAVAEDKSENVLDAHIPKTCAGRHSGQIPHTISQWNKPEAGPSRSDLSQLFPIASGEVMSDENHNEKCWEKNLPDSVKNHAINCNSLLQSHQSDRPQSQLCVACDSVSGEDLCLQTGISSPLERKVFPGIQLEMDDSPMNVSPLGNEPGLTGTRGPHPDSNMAVFHFRYEADRTISDAFHTLSEKLILDDCANCVTLPGGQQKKTCMAYTCKLVELTKTCGRKNGQLQCEPCSSLGDEHLCFESSCQQADDVLCSSRMFFREGFAHNPPAKTFLSPLEDFSDNCEDGDEFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSGHIGPAAWKHVEQRRARLIGPCGDVMEPLSAIDVRPRGTQHLTEAQWCLIYSAVRRGEETEDTIGSLLHCSRQLPTSETACGRIRDGPCLKQCVRDTECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSNFGGSTGRIMLKERQLGTSMANSNTVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRKVLPMDYLCFLTRDLSSPECQSSLPHLKASISASILTSQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSLEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEAALEFVADYSGQDNFLQRVGQNGLKNTEKESTVNSIFQIIRSCSRSLEMEEEDSSSEGAGSRKNSLKDKTRWQLIIGDFLDSENDIFEKSKECDLHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATAIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTSSPAGARPKFGVLSNMSEPGKFPSVGSPGLSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSLLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMIFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNVSPGMSTKNAEKSSIQRNNTLGISTTKKKKKMLMRGESGEATDDETATRKAKMCRECRSRSGSDPQDANEQEESEANVITNPPNPLHSRRAHSLTTAGSSNLTTGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSVRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENASDFQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSVSMCHQGLMSFEGFARFLMDKDNFASKNDESQENKRELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGSANPPPASNEEEEDEEDEYDYDYESLSDDNILEDKPENKSCADKLQFEYNEEIPKRIKKADNSSCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLIIYCQAVKFPGLSTLNSSGSSRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSNEGIRQTWEESCSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDSMDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNPLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEENPSGSTMPASLMFNTEERKCSQTHRVTVHGVPGPEPFAVFTINGGTKAKQLLQQVLVIDPDTKLSATDYFLMEEKYFISKEKNECKKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKSIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPGDYVLLEEVMKEAPSKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRKGISFASELKKLTKSTKQPRGLASPPQLVAPESVQIKEEKPVGALSSSDTVAYQQ

Target ORF information:

RefSeq Version	XM_005063631.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus phospholipase C epsilon 1 (Plce1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005063631.3

ORF Insert Sequence:

ATGACTTCCG AAGAAATGGC AGCTTCCATC CTCATCCCTG TGACCCAGAG GAAAGTGGCT 
TCTGCCCAGT CAGCAGTGGC AGAGGACAAG AGTGAAAATG TCTTGGATGC CCATATTCCA 
AAGACATGTG CTGGCAGGCA CAGCGGCCAG ATCCCTCATA CCATCTCACA GTGGAACAAA 
CCTGAGGCCG GGCCTTCTAG AAGTGATTTA TCCCAGTTAT TCCCAATAGC AAGTGGGGAA 
GTAATGAGTG ATGAGAACCA CAATGAAAAA TGCTGGGAGA AAAACCTACC AGATTCTGTG 
AAAAACCACG CCATTAACTG CAACAGCTTA CTGCAAAGCC ACCAGAGTGA CCGTCCTCAG 
AGTCAGCTTT GTGTAGCCTG TGACTCTGTC TCAGGAGAGG ACCTGTGTTT GCAAACTGGA 
ATTTCTTCTC CACTGGAAAG GAAGGTGTTC CCTGGGATTC AGCTGGAAAT GGATGATTCT 
CCTATGAATG TAAGCCCTTT GGGAAACGAG CCTGGACTCA CAGGGACCAG GGGACCTCAC 
CCTGACAGCA ACATGGCAGT ATTTCATTTC CGTTATGAAG CTGACAGAAC AATATCAGAT 
GCTTTCCACA CCCTGTCAGA AAAATTAATC TTGGATGACT GTGCAAATTG TGTGACTCTT 
CCCGGGGGGC AGCAGAAGAA AACTTGCATG GCATATACTT GTAAACTAGT GGAACTGACA 
AAAACCTGTG GCCGTAAGAA TGGGCAGCTG CAGTGTGAGC CTTGCAGCTC ACTGGGTGAC 
GAACACCTGT GCTTTGAAAG CTCCTGCCAG CAGGCTGATG ATGTGCTCTG CTCGAGCCGC 
ATGTTTTTCA GGGAGGGCTT TGCGCACAAT CCACCTGCCA AGACTTTTCT GAGCCCTTTG 
GAGGATTTCT CTGACAATTG TGAAGACGGA GACGAGTTTT TTAAAAGCAA AAAAGAACGG 
TCCACCTTGT TAGTCAGAAG GTTTTGTAAA AATGACAGGG AAGTTAAGAA GTCTGTGTAT 
ACTGGAACTA GAGCAATCAT GAGAACCCTG CCTTCCGGCC ATATTGGGCC AGCTGCTTGG 
AAACACGTTG AGCAGAGAAG AGCAAGGCTC ATAGGACCTT GTGGGGATGT AATGGAACCT 
CTGTCAGCAA TAGATGTAAG GCCACGTGGT ACCCAACATC TGACTGAAGC CCAGTGGTGT 
CTGATCTACA GTGCAGTGAG AAGAGGAGAA GAAACAGAAG ATACGATTGG ATCTCTTCTC 
CATTGCTCCA GACAGCTCCC AACCTCCGAG ACAGCCTGCG GAAGGATTAG AGACGGTCCA 
TGCTTAAAGC AATGTGTACG AGACACCGAA TGTGAGTACC GGGCCACCCT CCAAAGGACA 
TCCATTGCGC AGTACATCAC TGGCTCTCTC CTGGAGGCAA CCACCTCCTT AGGAGCAAGA 
AGCAGCCTTC TCAGTAACTT CGGAGGATCC ACTGGACGAA TAATGCTGAA AGAACGCCAA 
CTGGGCACCT CTATGGCCAA TTCCAATACT GTTCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATTG ATCTCCAGCC CCTCATTCAG TTCCCAGAGG AAGTGGCCAG CATCCTGACA 
GAGCAGGAGC AGAACATTTA CCGGAAGGTC CTGCCCATGG ACTACCTTTG TTTCTTAACC 
CGGGACTTGA GTTCCCCTGA ATGCCAGAGT TCCCTGCCCC ATCTCAAAGC GTCCATCTCA 
GCATCAATCC TCACTTCTCA GAATGGAGAG CACAATGCCC TTGAAGACCT TGTGATGAGA 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CAGCAGGCTC CATGGAGGAA 
AAGCGAGAAG TCTTCTCATA TTTGGTTCAC GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CCTGGCTGGC CTCAGGTCAA GGAAAGTTCT AAAGATGTGG 
CAGTTCATGG ATCAGTCTGA CATTGAGACC ATGCGGAGCC TGAAGGATGC CATGGCTCAG 
CACGAGTCCT CCCTGGAGTA CAAGAAGGTG GTGACTCGGG CCCTGCACAT CCCCGGCTGC 
AAGGTGGTCC CCTTCTGTGG TGTGTTCTTG AAGGAGCTCT GTGAGGTGCT TGATGGCGCC 
TCTGGCCTGC TGAAGCTCTG CCCGCGGTAC AGCTCCCAAG AAGCTGCTCT GGAATTTGTG 
GCTGATTACA GTGGACAAGA TAACTTCTTA CAACGAGTGG GACAGAATGG CTTGAAGAAC 
ACAGAGAAGG AGTCCACAGT CAACAGCATC TTTCAAATCA TCCGGAGCTG CAGTCGAAGC 
TTGGAGATGG AGGAGGAGGA CAGCTCCAGT GAAGGGGCTG GCTCCAGGAA AAACTCCTTG 
AAGGATAAAA CCCGATGGCA GTTGATAATT GGAGATTTTC TGGATTCAGA AAATGACATC 
TTTGAGAAGT CCAAAGAATG CGACCTTCAT GGATCAGAGG AGTCCCAGAA GGCCTTTGAC 
CATGGGACAG AGCTCATCCC GTGGTACGTG CTGTCCATCC AAGCTGATGT GCACCAGTTC 
CTATTGCAGG GGGCCACAGC CATCCACTAT GACCAGGACA CACATCTCTC TGCCCGCTGC 
TTCCTCCAGT TACAGCCTGA CAATAGCACC TTGACCTGGA TGAAGCCTCC CACCTCCTCC 
CCAGCTGGTG CCAGACCCAA GTTTGGTGTG CTCAGTAATA TGTCTGAACC TGGCAAGTTC 
CCGTCAGTGG GCAGTCCTGG GCTGAGTGGC CTGGCCGAGG GTATCCTGGA CCTGTTTTCG 
GTGAAGGCTG TGTACATGGG ACACCCGGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGAGTA GCCTGCTTCT CTCCGAGACT GGGGTAACAC TGCTCTATGG ACTGCAGACC 
ACTGACAATA GACTCTTGCA CTTTGTGGCA CCAAAGCACA CAGCTAAGAT GATTTTCAGT 
GGATTGCTGG AACTCACGAC AGCTGTGAGA AAGATAAGGA AGTTCCCTGA CCAAAGACAG 
CAGTGGCTTC GGAAACAGTA TGTCAGCCTC TACCAGGAGG ATGGTCGGTA TGAAGGCCCA 
ACCTTGGCTC ATGCTGTGGA GTTGTTTGGT GGCAGACGAT GGAGTGCTAG AAACGTCAGC 
CCTGGGATGT CAACAAAGAA TGCTGAGAAG TCCAGTATTC AGAGAAACAA CACCCTGGGT 
ATAAGCACCA CCAAGAAAAA GAAGAAAATG CTCATGAGGG GAGAGAGTGG AGAGGCAACT 
GACGATGAAA CAGCGACCCG CAAGGCCAAA ATGTGCCGAG AGTGTCGAAG CCGGAGCGGC 
TCTGATCCTC AGGACGCTAA TGAACAAGAG GAGTCGGAAG CCAATGTCAT CACCAACCCT 
CCCAACCCCC TCCATTCCAG AAGAGCTCAC TCTTTGACCA CGGCAGGGTC CTCCAACTTG 
ACTACTGGGA TGTCATCTCC CATCAGTGCC TGGAGCAGCA GCAGCTGGCA TGGGCGGATC 
AAAGGAGGGA TGAAGGGCTT CCAGAGCTTC ATGGTTTCGG ACAGTAACAT GAGCTTCATT 
GAGTTTGTCG AGCTGTTCAA GTCGTTCAGC GTAAGGAGCC GCAAGGACCT GAAGGACATC 
TTCGATATCT ACTCTGTGCC CTGCAATCGG TCTGCCTCTG AGTCAGCCCC CCTGTACACC 
AACCTGACCA TTGAAGAAAA CGCCAGTGAC TTTCAGCCAG ACCTAGATTT GCTAACCAGA 
AACGTCTCAG ACTTGGGGCT GTTCATTAAG AGCAAACAGC AACTTTCTGA CAACCAGAGG 
CAGATATCGG ATGCTATTGC AGCGGCGAGC ATTGTGACTA ATGGCACTGG GATTGAGAGC 
ACATCCCTGG GCATATTCGG GGTCGGCATA CTCCAGCTCA ATGACTTCCT GGTGAATTGC 
CAAGGAGAAC ACTGCACGTA TGATGAAATT CTCAGCATCA TCCAGAAATT CGAGCCTAGC 
GTCAGTATGT GTCATCAGGG CCTAATGTCG TTTGAAGGGT TTGCAAGGTT TCTGATGGAT 
AAAGATAATT TCGCCTCCAA AAATGATGAG TCACAAGAGA ACAAGAGAGA ACTGCAGCTA 
CCCCTCTCCT ACTACTATAT TGAATCTTCA CACAATACCT ACCTCACAGG CCATCAGCTC 
AAGGGAGAAT CCTCCGTGGA GCTGTACAGC CAGGTCCTCT TGCAAGGATG CAGGAGCATA 
GAGCTGGACT GCTGGGATGG AGATGATGGC ATGCCCATTA TTTACCATGG ACATACACTG 
ACGACCAAGA TCCCCTTCAA GGAAGTGGTG GAAGCCATTG ATCGCAGTGC CTTCATCACC 
TCTGACTTGC CAATCATCAT ATCCATTGAG AACCACTGTT CATTGCCTCA ACAACGAAAA 
ATGGCAGAAA TTTTCAAGAG TGTGTTTGGA GAAAAGCTGG TGGCTAAATT CTTATTCGAG 
ACAGATTTCT CAGATGATCC AATGCTTCCC TCACCTGACC AACTCAGGAA GAAAGTGCTT 
CTTAAAAACA AGAAGCTAAA GGCCCATCAG ACACCAGTCG ACATCCTAAA ACAAAAGGCT 
CATCAGTTAG CATCTATGCA AGCACAGGCT TTCACTGGTG GGAGTGCCAA CCCCCCACCT 
GCCAGTAATG AGGAAGAGGA AGACGAAGAG GATGAATATG ATTATGATTA TGAATCCCTT 
TCTGATGACA ATATTCTGGA AGACAAACCT GAAAACAAAT CATGTGCTGA CAAGCTTCAG 
TTTGAGTACA ATGAAGAAAT CCCCAAGAGG ATAAAGAAAG CAGATAACTC TTCTTGCAAT 
AAGGGAAAGG TATATGACAT GGAACTGGGG GAGGAGTTTT ACCTCCCTCA GAACAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTCTCT GACCTCATCA TCTACTGCCA GGCAGTAAAA 
TTTCCAGGCC TGTCGACTCT AAATTCATCT GGCTCTAGCA GAGGGAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCTGGC AGAATGAGCC CAGGGGAGAC AGCATCCTTC 
AACAGAACAT CTGGAAAAAG TTCCAACGAA GGAATTCGGC AAACCTGGGA GGAATCGTGT 
TCGCCACTCA GCCCAAGCAC ATCCCTCAGC GCTATCATTA GAACTCCCAA ATGCTATCAT 
ATCTCCTCAC TGAATGAAAA CGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTA 
ATCCAGCACA CCGCTTGTCA GCTGCTGAGG ACCTACCCGG CAGCGACCCG AATCGACTCA 
TCCAATCCCA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC CCTCAACTAC 
CAGACGGATG ATCTTCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGCTGT 
GGTTATGTTT TGAAGCCCCC TGTCCTGTGG GATAAAAGCT GCCCCATGTA CCAGAAGTTT 
TCTCCACTGG AAAGAGACCT AGACAGCATG GATCCTGCTA TCTATTCTCT AACTATCATC 
TCTGGCCAAA ATGTGTGCCC CAGTAACAGC ACCGGAAGTC CATGCATCGA GGTTGATGTC 
TTGGGCATGC CCCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATAG GAACCCCCTG 
AACCCCATGT GGAACGAACA GTTTCTCTTC CGGGTTCACT TTGAAGATCT TGTATTTCTT 
CGTTTTGCCG TTGTGGAAAA CAACAGTTCG GCAATAACGG CTCAGAGAAT CATTCCACTC 
AAAGCTTTAA AACGAGGTTA TCGACATCTT CAGCTGCGGA ACCTCCACAA TGAAATATTG 
GAAATCTCTA GCCTATTCAT AAATAGCAGA AGGATGGAAG AAAATCCCTC TGGCAGTACC 
ATGCCAGCCT CTTTGATGTT TAATACTGAA GAGAGGAAAT GTTCTCAGAC TCACAGAGTC 
ACAGTGCATG GTGTTCCAGG TCCGGAGCCC TTTGCTGTTT TCACCATAAA CGGAGGCACC 
AAGGCAAAGC AGCTTCTCCA ACAGGTTCTG GTGATTGACC CAGACACCAA ACTTAGTGCC 
ACAGACTACT TTCTGATGGA AGAGAAGTAT TTTATATCTA AGGAAAAGAA TGAGTGCAAG 
AAACAACCAT TCCAGAGAGC TGTGGGTCCA GAAGAAGATA TTGTGCAGAT TTTAAACAGC 
TGGTTTCCAG AAGAAGGCTA TGTAGGCAGG ATTGTCTTAA AGCCCCAGCA GGAAACCCTA 
GAAGAGAAAA GCATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCGGA GGAGGAGAGT 
TTCTTTGTCC AAGTTCATGA TGTCTCTCCA GAGCAACCCC GAACTGTCAT CAAAGCCCCT 
CGGGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGTATCCTGA ACAACCCCAA CCCAGGTGAC TATGTGCTTT TGGAAGAGGT GATGAAAGAA 
GCACCCAGTA AGAAGTCTTC TACTCCAAAG TCCTCACAGA GGATCCTTTT GGACCAAGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGGA AATTCATCCT GAAGCTAAAG 
GAGCAGGTTC AGGCATCCCG AGAAGATAAA AGGAAGGGCA TCTCCTTTGC AAGTGAACTC 
AAGAAACTCA CTAAGTCCAC CAAGCAGCCC CGAGGACTCG CATCACCTCC TCAGCTTGTG 
GCCCCAGAAA GTGTCCAAAT CAAGGAGGAG AAACCTGTAG GTGCCCTGTC CTCCAGTGAC 
ACAGTGGCTT ACCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLCE1 cDNA ORF clone, Mesocricetus auratus(Golden hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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