LOC101485565 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101485565
Entrez Gene ID	101485565
Full Name	voltage-dependent T-type calcium channel subunit alpha-1I
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012921716.2	XP_012777170.2	voltage-dependent T-type calcium channel subunit alpha-1I
XM_024802177.1	XP_024657945.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
XM_012921716.1	XP_012777170.1	voltage-dependent T-type calcium channel subunit alpha-1I-like isoform X2
XM_012921715.2	XP_012777169.1	voltage-dependent T-type calcium channel subunit alpha-1I-like isoform X1
XM_012921717.2	XP_012777171.1	voltage-dependent T-type calcium channel subunit alpha-1H-like isoform X3
XM_024802176.1	XP_024657944.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101485565 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101485565 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa03071	XM_012921717.2 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa03069	XM_012921715.2 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMa03070	XM_012921716.1 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMa312815	XM_012921716.2 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMa371937	XM_024802176.1 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMa371939	XM_024802177.1 Latest version!	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101485565 ( XM_012921717.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777171.1 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMa03071
Clone ID Related Accession (Same CDS sequence)	XM_012921717.2
Accession Version	XM_012921717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1H-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012921717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCACCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCAGAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777171.1
CDS	13..5556
Translation	MLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGKQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRSKENEGAEAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECHRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVRI

Target ORF information:

RefSeq Version	XM_012921717.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921717.2

ORF Insert Sequence:

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCACCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCAGAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101485565 ( XM_012921715.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777169.1 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X1, mRNA.

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CloneID	OMa03069
Clone ID Related Accession (Same CDS sequence)	XM_012921715.2
Accession Version	XM_012921715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1I-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012921715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAAG 
GCCAAGCTCG GGCGCTACTA TGACGACTAC TCTCCCCTGC GTCTGTCCAT CCACTCGCTG 
TGTACCAGCC ACCACCTGGA CCTCTTCATC ACTTTTATCA TCTGCGTCAA TGTCTTCACC 
ATGAGCATAG AGCACTATAA ACAACCAGTG TATTTAGGAG AGGTTCTGAA GTACTGTAAC 
TACGTGTTTA CCTGCATCTT CGTCGTTGAG GCCCTCCTCA AACTTGTGGC ATTTGGCATA 
CACAGGTTCA TCAGAGACAG GTGGAATCAG CTGGATTTAG CTATAGTGGC ATTGTCCATC 
ATGGGCATCG TCCTGGAGGA GCTGAAAATG AAAGGCACAT TACCAATAAA TCCAACCATC 
ATCAGGATCA TGAGAGTGCT CAGGATAGCA CGAGTGCTGA AGCTTCTAAA GATGGCCACA 
GGGATGAGAG CTCTGTTGGA TACTGTCATG CAAGCACTGC CGCAGGTGGG AAATCTTGGT 
CTTCTCTTCA TGCTGCTCTT CTTCATCTAT GCTGCTCTGG GAGTGGAGCT CTTTGGCAAA 
CTGGAGTGCA GCGTCGACAA CCCATGTGAA GGTCTGGGTC GTCACGCAAC CTTTAGAAAT 
TTTGGGATGG CCTTCCTCAC ACTGTTTCGA GTGTCCACTG GAGACAACTG GAATGGGATT 
ATGAAAGACA CATTGAGAGA GTGTCACCGG TCTGACGAAC ACTGTTTACC ATATCTGCCC 
TGGGTTTCTC CAATTTACTT TGTCACCTTT GTGCTCATGG CCCAGTTTGT TCTGGTCAAT 
GTGGTGGTGG CTGTTCTGAT GAAACATCTA GAGGAAAGCA ACAAGGATGC TAAAGAGGAT 
GCGGAGATTG ATGCAGAGAT CGCCTTGGAA ATGGATAGGG AGAGGGATCG CAGACTAAGC 
ACAAATTCCA ACAACAGCTC AGTCGGAGGG ACCTATGTGG GGCCATGCTC AAATTCACCT 
GAAGAGGAGG AAGAGGTGCA GTACAACAAC CAGAACCTGC TGTCATCCAA GATCTCTGTA 
TCCAGGATGC ATTCTCTGCC AAATGACAGT TATATGTTTA GACCGGTGCG ACCCGCCAGC 
GCCCCCTACC CTTTAGAAGA GGTAGACCTG AGTCCCCAAC ACCAGCATTC AGTCTCAGTG 
ATGTCGGTCC ACTCCCAGCC CTGTGAGAGC CACTCTCTGC TCCAGGTTCC GGACATTTCC 
CCCGTTCACC CTCGGTCACC TCCATCCCTG TCTCTTCCTC GCATCGCCCC GCCAACACCC 
AGCCCCTCAC CACGGGCCCT GCATAGGCAG GTGGCGGTTA AAGTTGATTC TGTGGAGTCT 
CAGGGTTTTG AGCAACAGGA GAGGACCAGC TCAGTCCATC TGCCCCCCCT GTCCCCTTCA 
CAGCTACCTC CTCATCCATC CTCCTTGTCA TCACCCCTGC CTGCTTCTCC CTCCTCCCCC 
TCCGCCTTCC CTCGCTATCG TCCTTCTCCT CTTCCTCCTC TGCTGTCTTC CCCTTCTTCT 
CTTCCTCCGC CACCGTCCTC CCCTTCGCTG CTTCTCCCTC CACCTCCCTC GCCCCGCGCC 
CCCTTGAGGC CTGCCAGCCT CCGGAGACCA AGGCCGCCCT CCGCCATCTC CCTCTGTGAC 
CCGGCCGATG AGGAGGTGTA TCACATCACC AGCTCTGCTG GCCACAGGTG GTGGGACGGG 
GAGGGAGGTG GGACGGAGAG CAGGGTTAAA GTGGGCCGCA GCCACTCCCT CTCCCCGTAC 
ACCTTGCCGT CTGCCCTCGA CTCCTACTCC TCCACCTTTT CTCCTCCTCC TCCTCCGCTG 
CCACCATCCT CGTCGAGGAG CGCACTGAGC CTGCTTGGAG CCGGACTGAA GGACCGGGAC 
TTCAGGAAAG GCTTCAGCAT CGACAACCAG GGCTTCCTGG ACAAGCCGCC ATCACTGAAT 
GCCGGATACG CCGACGACCA GCGGCGTCAC TCCATCGAGG TTTGCCTCCC AAGGGCTGGA 
ACAACCAGCT CTAACGAACA CTTCACAGAG GAGGCCTACT GGCTGGAGAA AGGCGGTCCT 
GTGAGGGTGC AGTCTGTCAG GAGCAACCAC AGAAAGAAGA AGATGAGTCC TCCATGTATA 
TCCATTCACC CACCCTCAGA GAGGGAGCAC GCTGAAACCG CCTCGCCCCC CAAATTATCT 
GATTGCAGCA CGATGCTGAG ACGCAGGACA CCCTCCTACG ACTTGACGCC ACACATGCAG 
TCAAATACAA ACGCCCAAGA CGCGTCATCA GATGCAAACA CGCAATCTCC AATGCACAGA 
TCGCTAACGC CTGTCCATGT TCCTCTGCGA GCAAACTCGC CCACTTGGGC ACTGGCTCAC 
TCGCGACCGT CTACCCCAAC ACGCACTCAG ACACACACCC CTCCCCACGC CTACACGCCT 
ACACACCCAC ACATCCCCAT CAGCCCGAAC GTCTTTATCC AGCCTCACAC CCCTCTTGTC 
CCAATGCCTT TCTCACAGAC ACGCCCTGTC TCCCCTGCTG CGGGGCACAT CCCCCTCCCT 
CAGTTCACCT TCGACCAACC GCAGTCCAGA TACATGAGCG GCCTATCAGC TGGCATATCA 
GACACCGACA CGGCCACCTG CTCTTCCCCT TTCGACGGCA GACTGGGAAG CGGCGCAGGA 
TTCAGTGCAA AGAATCGGAG GACCGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777169.1
CDS	13..7065
Translation	MLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGKQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRKAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECHRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVSVMSVHSQPCESHSLLQVPDISPVHPRSPPSLSLPRIAPPTPSPSPRALHRQVAVKVDSVESQGFEQQERTSSVHLPPLSPSQLPPHPSSLSSPLPASPSSPSAFPRYRPSPLPPLLSSPSSLPPPPSSPSLLLPPPPSPRAPLRPASLRRPRPPSAISLCDPADEEVYHITSSAGHRWWDGEGGGTESRVKVGRSHSLSPYTLPSALDSYSSTFSPPPPPLPPSSSRSALSLLGAGLKDRDFRKGFSIDNQGFLDKPPSLNAGYADDQRRHSIEVCLPRAGTTSSNEHFTEEAYWLEKGGPVRVQSVRSNHRKKKMSPPCISIHPPSEREHAETASPPKLSDCSTMLRRRTPSYDLTPHMQSNTNAQDASSDANTQSPMHRSLTPVHVPLRANSPTWALAHSRPSTPTRTQTHTPPHAYTPTHPHIPISPNVFIQPHTPLVPMPFSQTRPVSPAAGHIPLPQFTFDQPQSRYMSGLSAGISDTDTATCSSPFDGRLGSGAGFSAKNRRTAQ

Target ORF information:

RefSeq Version	XM_012921715.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921715.2

ORF Insert Sequence:

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAAG 
GCCAAGCTCG GGCGCTACTA TGACGACTAC TCTCCCCTGC GTCTGTCCAT CCACTCGCTG 
TGTACCAGCC ACCACCTGGA CCTCTTCATC ACTTTTATCA TCTGCGTCAA TGTCTTCACC 
ATGAGCATAG AGCACTATAA ACAACCAGTG TATTTAGGAG AGGTTCTGAA GTACTGTAAC 
TACGTGTTTA CCTGCATCTT CGTCGTTGAG GCCCTCCTCA AACTTGTGGC ATTTGGCATA 
CACAGGTTCA TCAGAGACAG GTGGAATCAG CTGGATTTAG CTATAGTGGC ATTGTCCATC 
ATGGGCATCG TCCTGGAGGA GCTGAAAATG AAAGGCACAT TACCAATAAA TCCAACCATC 
ATCAGGATCA TGAGAGTGCT CAGGATAGCA CGAGTGCTGA AGCTTCTAAA GATGGCCACA 
GGGATGAGAG CTCTGTTGGA TACTGTCATG CAAGCACTGC CGCAGGTGGG AAATCTTGGT 
CTTCTCTTCA TGCTGCTCTT CTTCATCTAT GCTGCTCTGG GAGTGGAGCT CTTTGGCAAA 
CTGGAGTGCA GCGTCGACAA CCCATGTGAA GGTCTGGGTC GTCACGCAAC CTTTAGAAAT 
TTTGGGATGG CCTTCCTCAC ACTGTTTCGA GTGTCCACTG GAGACAACTG GAATGGGATT 
ATGAAAGACA CATTGAGAGA GTGTCACCGG TCTGACGAAC ACTGTTTACC ATATCTGCCC 
TGGGTTTCTC CAATTTACTT TGTCACCTTT GTGCTCATGG CCCAGTTTGT TCTGGTCAAT 
GTGGTGGTGG CTGTTCTGAT GAAACATCTA GAGGAAAGCA ACAAGGATGC TAAAGAGGAT 
GCGGAGATTG ATGCAGAGAT CGCCTTGGAA ATGGATAGGG AGAGGGATCG CAGACTAAGC 
ACAAATTCCA ACAACAGCTC AGTCGGAGGG ACCTATGTGG GGCCATGCTC AAATTCACCT 
GAAGAGGAGG AAGAGGTGCA GTACAACAAC CAGAACCTGC TGTCATCCAA GATCTCTGTA 
TCCAGGATGC ATTCTCTGCC AAATGACAGT TATATGTTTA GACCGGTGCG ACCCGCCAGC 
GCCCCCTACC CTTTAGAAGA GGTAGACCTG AGTCCCCAAC ACCAGCATTC AGTCTCAGTG 
ATGTCGGTCC ACTCCCAGCC CTGTGAGAGC CACTCTCTGC TCCAGGTTCC GGACATTTCC 
CCCGTTCACC CTCGGTCACC TCCATCCCTG TCTCTTCCTC GCATCGCCCC GCCAACACCC 
AGCCCCTCAC CACGGGCCCT GCATAGGCAG GTGGCGGTTA AAGTTGATTC TGTGGAGTCT 
CAGGGTTTTG AGCAACAGGA GAGGACCAGC TCAGTCCATC TGCCCCCCCT GTCCCCTTCA 
CAGCTACCTC CTCATCCATC CTCCTTGTCA TCACCCCTGC CTGCTTCTCC CTCCTCCCCC 
TCCGCCTTCC CTCGCTATCG TCCTTCTCCT CTTCCTCCTC TGCTGTCTTC CCCTTCTTCT 
CTTCCTCCGC CACCGTCCTC CCCTTCGCTG CTTCTCCCTC CACCTCCCTC GCCCCGCGCC 
CCCTTGAGGC CTGCCAGCCT CCGGAGACCA AGGCCGCCCT CCGCCATCTC CCTCTGTGAC 
CCGGCCGATG AGGAGGTGTA TCACATCACC AGCTCTGCTG GCCACAGGTG GTGGGACGGG 
GAGGGAGGTG GGACGGAGAG CAGGGTTAAA GTGGGCCGCA GCCACTCCCT CTCCCCGTAC 
ACCTTGCCGT CTGCCCTCGA CTCCTACTCC TCCACCTTTT CTCCTCCTCC TCCTCCGCTG 
CCACCATCCT CGTCGAGGAG CGCACTGAGC CTGCTTGGAG CCGGACTGAA GGACCGGGAC 
TTCAGGAAAG GCTTCAGCAT CGACAACCAG GGCTTCCTGG ACAAGCCGCC ATCACTGAAT 
GCCGGATACG CCGACGACCA GCGGCGTCAC TCCATCGAGG TTTGCCTCCC AAGGGCTGGA 
ACAACCAGCT CTAACGAACA CTTCACAGAG GAGGCCTACT GGCTGGAGAA AGGCGGTCCT 
GTGAGGGTGC AGTCTGTCAG GAGCAACCAC AGAAAGAAGA AGATGAGTCC TCCATGTATA 
TCCATTCACC CACCCTCAGA GAGGGAGCAC GCTGAAACCG CCTCGCCCCC CAAATTATCT 
GATTGCAGCA CGATGCTGAG ACGCAGGACA CCCTCCTACG ACTTGACGCC ACACATGCAG 
TCAAATACAA ACGCCCAAGA CGCGTCATCA GATGCAAACA CGCAATCTCC AATGCACAGA 
TCGCTAACGC CTGTCCATGT TCCTCTGCGA GCAAACTCGC CCACTTGGGC ACTGGCTCAC 
TCGCGACCGT CTACCCCAAC ACGCACTCAG ACACACACCC CTCCCCACGC CTACACGCCT 
ACACACCCAC ACATCCCCAT CAGCCCGAAC GTCTTTATCC AGCCTCACAC CCCTCTTGTC 
CCAATGCCTT TCTCACAGAC ACGCCCTGTC TCCCCTGCTG CGGGGCACAT CCCCCTCCCT 
CAGTTCACCT TCGACCAACC GCAGTCCAGA TACATGAGCG GCCTATCAGC TGGCATATCA 
GACACCGACA CGGCCACCTG CTCTTCCCCT TTCGACGGCA GACTGGGAAG CGGCGCAGGA 
TTCAGTGCAA AGAATCGGAG GACCGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101485565 ( XM_012921716.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777170.1 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X2, mRNA.

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CloneID	OMa03070
Clone ID Related Accession (Same CDS sequence)	XM_012921716.1
Accession Version	XM_012921716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1I-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCACCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCTCAGT GATGTCGGTC CACTCCCAGC CCTGTGAGAG CCACTCTCTG 
CTCCAGGTTC CGGACATTTC CCCCGTTCAC CCTCGGTCAC CTCCATCCCT GTCTCTTCCT 
CGCATCGCCC CGCCAACACC CAGCCCCTCA CCACGGGCCC TGCATAGGCA GGTGGCGGTT 
AAAGTTGATT CTGTGGAGTC TCAGGGTTTT GAGCAACAGG AGAGGACCAG CTCAGTCCAT 
CTGCCCCCCC TGTCCCCTTC ACAGCTACCT CCTCATCCAT CCTCCTTGTC ATCACCCCTG 
CCTGCTTCTC CCTCCTCCCC CTCCGCCTTC CCTCGCTATC GTCCTTCTCC TCTTCCTCCT 
CTGCTGTCTT CCCCTTCTTC TCTTCCTCCG CCACCGTCCT CCCCTTCGCT GCTTCTCCCT 
CCACCTCCCT CGCCCCGCGC CCCCTTGAGG CCTGCCAGCC TCCGGAGACC AAGGCCGCCC 
TCCGCCATCT CCCTCTGTGA CCCGGCCGAT GAGGAGGTGT ATCACATCAC CAGCTCTGCT 
GGCCACAGGT GGTGGGACGG GGAGGGAGGT GGGACGGAGA GCAGGGTTAA AGTGGGCCGC 
AGCCACTCCC TCTCCCCGTA CACCTTGCCG TCTGCCCTCG ACTCCTACTC CTCCACCTTT 
TCTCCTCCTC CTCCTCCGCT GCCACCATCC TCGTCGAGGA GCGCACTGAG CCTGCTTGGA 
GCCGGACTGA AGGACCGGGA CTTCAGGAAA GGCTTCAGCA TCGACAACCA GGGCTTCCTG 
GACAAGCCGC CATCACTGAA TGCCGGATAC GCCGACGACC AGCGGCGTCA CTCCATCGAG 
GTTTGCCTCC CAAGGGCTGG AACAACCAGC TCTAACGAAC ACTTCACAGA GGAGGCCTAC 
TGGCTGGAGA AAGGCGGTCC TGTGAGGGTG CAGTCTGTCA GGAGCAACCA CAGAAAGAAG 
AAGATGAGTC CTCCATGTAT ATCCATTCAC CCACCCTCAG AGAGGGAGCA CGCTGAAACC 
GCCTCGCCCC CCAAATTATC TGATTGCAGC ACGATGCTGA GACGCAGGAC ACCCTCCTAC 
GACTTGACGC CACACATGCA GTCAAATACA AACGCCCAAG ACGCGTCATC AGATGCAAAC 
ACGCAATCTC CAATGCACAG ATCGCTAACG CCTGTCCATG TTCCTCTGCG AGCAAACTCG 
CCCACTTGGG CACTGGCTCA CTCGCGACCG TCTACCCCAA CACGCACTCA GACACACACC 
CCTCCCCACG CCTACACGCC TACACACCCA CACATCCCCA TCAGCCCGAA CGTCTTTATC 
CAGCCTCACA CCCCTCTTGT CCCAATGCCT TTCTCACAGA CACGCCCTGT CTCCCCTGCT 
GCGGGGCACA TCCCCCTCCC TCAGTTCACC TTCGACCAAC CGCAGTCCAG ATACATGAGC 
GGCCTATCAG CTGGCATATC AGACACCGAC ACGGCCACCT GCTCTTCCCC TTTCGACGGC 
AGACTGGGAA GCGGCGCAGG ATTCAGTGCA AAGAATCGGA GGACCGCCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777170.1
CDS	16..7089
Translation	MLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGKQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRSKENEGAEAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECHRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVSVMSVHSQPCESHSLLQVPDISPVHPRSPPSLSLPRIAPPTPSPSPRALHRQVAVKVDSVESQGFEQQERTSSVHLPPLSPSQLPPHPSSLSSPLPASPSSPSAFPRYRPSPLPPLLSSPSSLPPPPSSPSLLLPPPPSPRAPLRPASLRRPRPPSAISLCDPADEEVYHITSSAGHRWWDGEGGGTESRVKVGRSHSLSPYTLPSALDSYSSTFSPPPPPLPPSSSRSALSLLGAGLKDRDFRKGFSIDNQGFLDKPPSLNAGYADDQRRHSIEVCLPRAGTTSSNEHFTEEAYWLEKGGPVRVQSVRSNHRKKKMSPPCISIHPPSEREHAETASPPKLSDCSTMLRRRTPSYDLTPHMQSNTNAQDASSDANTQSPMHRSLTPVHVPLRANSPTWALAHSRPSTPTRTQTHTPPHAYTPTHPHIPISPNVFIQPHTPLVPMPFSQTRPVSPAAGHIPLPQFTFDQPQSRYMSGLSAGISDTDTATCSSPFDGRLGSGAGFSAKNRRTAQ

Target ORF information:

RefSeq Version	XM_012921716.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1H-like (LOC101485565), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921716.1

ORF Insert Sequence:

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTACCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGAAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCACCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCTCAGT GATGTCGGTC CACTCCCAGC CCTGTGAGAG CCACTCTCTG 
CTCCAGGTTC CGGACATTTC CCCCGTTCAC CCTCGGTCAC CTCCATCCCT GTCTCTTCCT 
CGCATCGCCC CGCCAACACC CAGCCCCTCA CCACGGGCCC TGCATAGGCA GGTGGCGGTT 
AAAGTTGATT CTGTGGAGTC TCAGGGTTTT GAGCAACAGG AGAGGACCAG CTCAGTCCAT 
CTGCCCCCCC TGTCCCCTTC ACAGCTACCT CCTCATCCAT CCTCCTTGTC ATCACCCCTG 
CCTGCTTCTC CCTCCTCCCC CTCCGCCTTC CCTCGCTATC GTCCTTCTCC TCTTCCTCCT 
CTGCTGTCTT CCCCTTCTTC TCTTCCTCCG CCACCGTCCT CCCCTTCGCT GCTTCTCCCT 
CCACCTCCCT CGCCCCGCGC CCCCTTGAGG CCTGCCAGCC TCCGGAGACC AAGGCCGCCC 
TCCGCCATCT CCCTCTGTGA CCCGGCCGAT GAGGAGGTGT ATCACATCAC CAGCTCTGCT 
GGCCACAGGT GGTGGGACGG GGAGGGAGGT GGGACGGAGA GCAGGGTTAA AGTGGGCCGC 
AGCCACTCCC TCTCCCCGTA CACCTTGCCG TCTGCCCTCG ACTCCTACTC CTCCACCTTT 
TCTCCTCCTC CTCCTCCGCT GCCACCATCC TCGTCGAGGA GCGCACTGAG CCTGCTTGGA 
GCCGGACTGA AGGACCGGGA CTTCAGGAAA GGCTTCAGCA TCGACAACCA GGGCTTCCTG 
GACAAGCCGC CATCACTGAA TGCCGGATAC GCCGACGACC AGCGGCGTCA CTCCATCGAG 
GTTTGCCTCC CAAGGGCTGG AACAACCAGC TCTAACGAAC ACTTCACAGA GGAGGCCTAC 
TGGCTGGAGA AAGGCGGTCC TGTGAGGGTG CAGTCTGTCA GGAGCAACCA CAGAAAGAAG 
AAGATGAGTC CTCCATGTAT ATCCATTCAC CCACCCTCAG AGAGGGAGCA CGCTGAAACC 
GCCTCGCCCC CCAAATTATC TGATTGCAGC ACGATGCTGA GACGCAGGAC ACCCTCCTAC 
GACTTGACGC CACACATGCA GTCAAATACA AACGCCCAAG ACGCGTCATC AGATGCAAAC 
ACGCAATCTC CAATGCACAG ATCGCTAACG CCTGTCCATG TTCCTCTGCG AGCAAACTCG 
CCCACTTGGG CACTGGCTCA CTCGCGACCG TCTACCCCAA CACGCACTCA GACACACACC 
CCTCCCCACG CCTACACGCC TACACACCCA CACATCCCCA TCAGCCCGAA CGTCTTTATC 
CAGCCTCACA CCCCTCTTGT CCCAATGCCT TTCTCACAGA CACGCCCTGT CTCCCCTGCT 
GCGGGGCACA TCCCCCTCCC TCAGTTCACC TTCGACCAAC CGCAGTCCAG ATACATGAGC 
GGCCTATCAG CTGGCATATC AGACACCGAC ACGGCCACCT GCTCTTCCCC TTTCGACGGC 
AGACTGGGAA GCGGCGCAGG ATTCAGTGCA AAGAATCGGA GGACCGCCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101485565 ( XM_012921716.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777170.2 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), mRNA.

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CloneID	OMa312815
Clone ID Related Accession (Same CDS sequence)	XM_012921716.2
Accession Version	XM_012921716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012921716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACTGATG GCGAAGGGAG AGCGCGCTTT CAGTACGCAG AGTGCCAGGA GCTGGAGGAG 
GTGCGGGAGG AGGTAAGGCT TGCTGTACCG ACATCCTACA GGAATGACGG CCAAGGTCAT 
GAAGATGACC CAGGAAGAAG ACGCCCGGAT GATGAGGAGG AAGAGGAGGA GGTGGAGAGG 
ACGAGAAGGA GCAGCAGGAG GAGGAGGAGG AGGAGCAGCA GCAGTAGCAT TCAAGGAGAT 
GGAGAAAATG AGTGGAGTCC TGAAGTTCAG GGGGATGAAG AACAGGAACC GGAGCTGGAA 
GTGGAAGATG GACCAACACT GGGAGTTCGC ATCACGGCTC CCATCCGTGA TCCAGACTGC 
GTCTCCGAGG AGGAGGAACA GCAGCCGTAT CCGGCCCTGG CCCCCGTAGC CTTCTTCTGC 
TTAAAGCAAA CAACGAGGCC TCGCAACTGG TGTCTCCGCA TGGTCTGCAA TCCATATCCT 
TTAACATGCT GGAAATCTGA CCCTGGTAAT CGGTGGTTTG AGCACGTGTC CATGTTGGTG 
ATCCTGCTCA ACTGTGTGAC TCTGGGGATG TATCGGCCCT GTGAGGACGT TACCTGCCAG 
TCAGAGTGGT GTCGCATCTT ACAGGTGTTG GATGACTGCA TCTTCGCTTT CTTTGCGGTG 
GAAATGGTTA TCAAGATGGT GGCCCTGGGA ATTTTTGGCT CCAAGTGTTA CTTTGATGAC 
AAGTGGAATC AGCTTGACTT TGTCATTGTC ATGGCAGGGA TGTTGGAGTA TTCCCTGGAT 
GGCCACAATG CCAGTCTGTC AGCTATCCGT ACCGTCCGAG TACTCAGGCC ACTGCGGGCT 
ATCAACAGAG TACCAAGTAT GAGGATCTTG GTGACACTGC TGTTAGACAC TCTGCCGATG 
CTGGGAAATG TTCTGCTCCT CTGCTTCTTT GTCTTCTTCA TCTTCGGCAT TGTGGGAGTC 
CAGTTGTGGG AGGGACTGCT GCGCAATCGC TGCTTCCTGG GAGAGGACAT CGAAACAATG 
TACAACATGT CTTTAAATCC TTATTACATG TCTGAGGAAG GTGAAGACAG CCCGTTTATC 
TGCTCGGCTC CACGCGAAAA CGGGATGCGG CGTTGCAAAG ATGTGCCACC CTACTCTCAA 
GACGGGCTCG AATGCACATT GCCGGCTTCT GACCTGAGTT TTAGGAGTGT TGCCAGCGGT 
AACTTTTGTG TTAACTGGTA TCAGTACTAC AACGACTGCC GCCCAGGGGA GCTCAACCCT 
CACAGGGGAG CTGTCAACTT TGACAATATC GGCTATGCTT GGATAGCCAT ATTCCAGGTC 
ATCACACTGG AAGGCTGGGT AGACATCATG TACTATGTCA TGGATGCTCA CTCCTTCTAT 
AATTTTATAT ATTTTATACT GCTCATTATT GTGGGCTCTT TCTTCATGAT CAACTTGTGC 
CTCGTGGTCA TTGCCACCCA ATTCTCCGAG ACAAAGCAAA GGGAGAATGC ACTGATGCGG 
GAGCAGCGTG CTCGTTACAT GTCAAACGAC TCCACACTTG CCAGTTACAG CGAGCCGGGC 
TCGTGCTATG AAGAGATGCT ACGCTACATT AGCCATCTTT ACCGCAAGTT TACACGCAGG 
CTCCAGAGGA TATACTCTGG CTGGCACAGT AAACGTCGTA AAAAGGTGAA TCCTAATGGC 
AGCAGTGGAG GCAGTAATGG TGGAGGTAAT GGGCATGGCC ACGGTTCCAG CAGGGGCTGC 
GGAGGGTCAG GCCCAGGTAA CGCATTGTGG TTGAGGCCAA TCCATAACCT TTTACAACAC 
CATCAGCATC AGCACTGTCG TCTCAGCAAT GGCGGGCAGC ACACCGTCAG CGTGGCAACC 
ACAGAGCACG TGGACAGAGA AGGACACATC AAAGTCGGGG AGGAACTGGA GATGAAGTCG 
CTTACCGTTC GGAGGGGAAG TGCAAATGGA AACAACGATA ACTCGACCAC TGTCACCCAG 
CGCGTGGGCT CATTACTCGG GCGACTGAAC GGGGGGATGA ACTATCCCAC CATTCTGCCG 
TCTTTTGTGT GCAGCTATAG CAGCAAGACG CCACCCCCTC ACTCAAAGCA GTGCGGTTCT 
TGTCTGGAGC ATCCGCCGAA CCACCCGGCT TCTGAGCACG CCGAGACACT GACCAAGCTT 
TACCAGCTCA TGGGACAGCA CAGTCGCCAG CGGCTGCTCT ACCAGCTGGC GGGTATGGTG 
CCCAGCCCTC TTCTCTTGGA GCTACTTAGC TGCCCTTTGT GTGCCAGCAG CCTGGAGACC 
TTGGAGCTGG AACTGGGAGA CCTAGAGGGA GGCAAGGGAG AGGCAGATGG ACTACACGAC 
TTCCCTCAGG GGGCCGATGT GAGAGGAGTG AGGGGTCGGA GCAGACGCCG TGGGGTTGTT 
GAATACGAAA ATGGAGTGGT TCGTGCCTGG AGGGACCTCA GGGAAAAACT AAAGAGGATT 
GTGGAAAGTA AATACTTCAA CCGCGGGATC ATGATCGCTA TTCTTGTGAA TACTCTCAGC 
ATGGGAATAG AGTACCACGA GCAGCCCGAG GAACTGACTG ATGTGTTAGA GATCAGCAAC 
ATCGTTTTCA CCAGCATGTT TGTCCTGGAG ATGGGTTTCA TGCTGCTGGC TTTCGGCTTG 
TTTGGATACA TCAGGAACCC ATACAACATC TTTGACAGCA TCATTGTTAT CATCAGTGTG 
TGGGAGATAA TTGGCCAAGC AGACGGTGGG CTGTCAGTCC TGCGAACGTT TCGTCTGCTA 
CGAGTTCTGA AGCTGGTTCG CTTCCTACCC GCTCTGAGGA GACAACTCGT AGTGCTGATG 
AAGACAATGG ACAATGTGGC CACCTTCTGC ATGCTTCTCA TGCTTTTCAT CTTCACTTTT 
AGTATACTGG GCATGCATCT TTTTGGGTGT AAATTTAGTT TTCGCACAGA GAATGGAGAC 
ACCATCCCTG ACCGAAAGAA CTTTGACAGT CTGCTCTGGG CCATTGTCAC TGTGTTCCAG 
ATTCTTACCC AGGAGGACTG GAATGTGGTG TTGTATAACG GCATGGCTTC TACCTCTCCA 
TGGGCGGCTC TCTACTTCGT TGCTCTTATG ACATTTGGCA ACTATGTGCT CTTCAATTTA 
CTAGTGGCCA TCTTGGTTGA AGGCTTCCAG GCTGAGGGTG ACGCTAATCG GTCAGAGTCA 
GATGATGACA AGAAATCAGA CAACTTTGAT GAGGACGAGA AGATAGAACA GTTCAGAGAC 
ACAGAGCTAA AGCTCTCACT GATGATGACA GGTAATGGTC ACATTGACCC TCGTGGCTCT 
ATGGCTCCTC CCATAATCAT GCGCACAGCA GCCACACCCA TGCCTACACC TAAGAGCTCT 
CTTGGACCTG AGTCCATCCT CGGGGATGAG CTCATGTACA TAGACGGAGC ACCTCAATAT 
AGTGAGGTAG GACTGGGTTT TCCTGAGGCA GGGGTCGGTC ACCCAGAGTC CCGACGGGGA 
AGCTCAACAT CAGTGGAGCC ATACGATCCG CCTTCTCTGA GCCTCTCTAG TCCCGGGCGG 
CGTTCTCCTC TTCCTCCCCG TGGCGCTGGA AGCTCATGGG GAAGTCGACG CTCCAGCTGG 
AACAGCCTGG GAAGGGCACC GAGCCTCAAA AGGCGAGACA CCAGCGGAGA GCGGGAGAGC 
TTGTTGTCTG GGGAAGGTGG AGAGGATGAG GATGCCAGGG ATGGCAAAGT GGAGGCAGGG 
GGAAACAACA GACTAAAGCC GGGGTCCTTG GAACTGCTGC AGGCGTCTAC TCTCTGCCCT 
TCTGTGGTAG CAGACTATGG TGACTGCAAT GGGAGGACCG TGACCTACAA CCCGAACGTG 
AACTCCGACC CCAAAGAAGA CTCCTCCCCA GAGGACGATT TGGACGATGA TGCTTCTTTT 
TGCTACTGGA CCAGGAAGGA GTTCCATAAC ATGAAGCCCC GATGGTGCAA AGAGCATGAA 
GACTGGACGC TGTACCTGTT TTCTCCAGAG AATCCGTTCC GTATGTGGTG CCAGAAAGTG 
ATCTCTCACA GACTGTTTGA CCACATAGTT CTAGTTTTCA TCTTTCTCAA CTGCATCACC 
ATTGCTCTGG AAAGACCTGA TATAAAAAAT GACAGCACGG AGCGACATTT CCTGAGTGTG 
TCCAACTATA TTTTCACTGT GATCTTCCTA GCAGAGATGG CCATTAAGGT TGTAGCTCTT 
GGTTTCTGCT TTGGGAAGCA GAGCTACCTC CAGTCCAGTT GGAACATTCT GGATGGTGTA 
TTGGTGTTTG TGTCTCTGAT CGACATTTTG GTTTATCTGG CCTATCATGG TGAAAACAAG 
ATCCTGGGAA TTCTGAGAGT ACTTCGCCTG CTACGAACAC TTCGCCCACT GAGGGTGATC 
AGTCGAGCCC CAGGATTAAA GCTTGTTGTG GAGACTCTCA TCACCTCTCT TAGACCTATT 
GGGAACATTG TTTTGATATG CTGCGCCTTC TTCATTATCT TTGGAATCTT GGGGGTCCAG 
CTGTTCAAAG GGAAGTTCTA CCACTGTGAA GGTCTGGACA CAAGAAACAT CACCAACAAA 
TCAGACTGTC TGCAGGCTAA CTACCGCTGG ATACGAAAAA AGTACAACTT TGACAACCTG 
ATTCAGGCTC TGATGTCTTT GTTTGTGCTG TCGTGTAAGG ATGGCTGGGT TACCATCATG 
TATGATGGGC TGGATGCAGT AGGAGTGGAC CAACAGCCTA AAAGAAACAA CAATCCTTGG 
ATGCTCCTCT ACTTTATCTC CTTCCTGCTC ATCGTCAGCT TCTTCGTCCT TAATATGTTT 
GTTGGGGTTG TGGTGGAGAA TTTCCACAAG TGCCGACAGG ACCAGGAAGA GGAGGAGGCG 
CGCTTACGGG AAGAGAAGAG GCTCAAAATG AAAGAGAAAA AGCGCAGGAG TAAGGAGAAT 
GAAGGGGCTG AGGCCAAGCT CGGGCGCTAC TATGACGACT ACTCTCCCCT GCGTCTGTCC 
ATCCACTCGC TGTGTACCAG CCACCACCTG GACCTCTTCA TCACTTTTAT CATCTGCGTC 
AATGTCTTCA CCATGAGCAT AGAGCACTAT AAACAACCAG TGTATTTAGG AGAGGTTCTG 
AAGTACTGTA ACTACGTGTT TACCTGCATC TTCGTCGTTG AGGCCCTCCT CAAACTTGTG 
GCATTTGGCA TACACAGGTT CATCAGAGAC AGGTGGAATC AGCTGGATTT AGCTATAGTG 
GCATTGTCCA TCATGGGCAT CGTCCTGGAG GAGCTGAAAA TGAAAGGCAC ATTACCAATA 
AATCCAACCA TCATCAGGAT CATGAGAGTG CTCAGGATAG CACGAGTGCT GAAGCTTCTA 
AAGATGGCCA CAGGGATGAG AGCTCTGTTG GATACTGTCA TGCAAGCACT GCCGCAGGTG 
GGAAATCTTG GTCTTCTCTT CATGCTGCTC TTCTTCATCT ATGCTGCTCT GGGAGTGGAG 
CTCTTTGGCA AACTGGAGTG CAGCGTCGAC AACCCATGTG AAGGTCTGGG TCGTCACGCA 
ACCTTTAGAA ATTTTGGGAT GGCCTTCCTC ACACTGTTTC GAGTGTCCAC TGGAGACAAC 
TGGAATGGGA TTATGAAAGA CACATTGAGA GAGTGTCACC GGTCTGACGA ACACTGTTTA 
CCATATCTGC CCTGGGTTTC TCCAATTTAC TTTGTCACCT TTGTGCTCAT GGCCCAGTTT 
GTTCTGGTCA ATGTGGTGGT GGCTGTTCTG ATGAAACATC TAGAGGAAAG CAACAAGGAT 
GCTAAAGAGG ATGCGGAGAT TGATGCAGAG ATCGCCTTGG AAATGGATAG GGAGAGGGAT 
CGCAGACTAA GCACAAATTC CAACAACAGC TCAGTCGGAG GGACCTATGT GGGGCCATGC 
TCAAATTCAC CTGAAGAGGA GGAAGAGGTG CAGTACAACA ACCAGAACCT GCTGTCATCC 
AAGATCTCTG TATCCAGGAT GCATTCTCTG CCAAATGACA GTTATATGTT TAGACCGGTG 
CGACCCGCCA GCGCCCCCTA CCCTTTAGAA GAGGTAGACC TGAGTCCCCA ACACCAGCAT 
TCAGTCTCAG TGATGTCGGT CCACTCCCAG CCCTGTGAGA GCCACTCTCT GCTCCAGGTT 
CCGGACATTT CCCCCGTTCA CCCTCGGTCA CCTCCATCCC TGTCTCTTCC TCGCATCGCC 
CCGCCAACAC CCAGCCCCTC ACCACGGGCC CTGCATAGGC AGGTGGCGGT TAAAGTTGAT 
TCTGTGGAGT CTCAGGGTTT TGAGCAACAG GAGAGGACCA GCTCAGTCCA TCTGCCCCCC 
CTGTCCCCTT CACAGCTACC TCCTCATCCA TCCTCCTTGT CATCACCCCT GCCTGCTTCT 
CCCTCCTCCC CCTCCGCCTT CCCTCGCTAT CGTCCTTCTC CTCTTCCTCC TCTGCTGTCT 
TCCCCTTCTT CTCTTCCTCC GCCACCGTCC TCCCCTTCGC TGCTTCTCCC TCCACCTCCC 
TCGCCCCGCG CCCCCTTGAG GCCTGCCAGC CTCCGGAGAC CAAGGCCGCC CTCCGCCATC 
TCCCTCTGTG ACCCGGCCGA TGAGGAGGTG TATCACATCA CCAGCTCTGC TGGCCACAGG 
TGGTGGGACG GGGAGGGAGG TGGGACGGAG AGCAGGGTTA AAGTGGGCCG CAGCCACTCC 
CTCTCCCCGT ACACCTTGCC GTCTGCCCTC GACTCCTACT CCTCCACCTT TTCTCCTCCT 
CCTCCTCCGC TGCCACCATC CTCGTCGAGG AGCGCACTGA GCCTGCTTGG AGCCGGACTG 
AAGGACCGGG ACTTCAGGAA AGGCTTCAGC ATCGACAACC AGGGCTTCCT GGACAAGCCG 
CCATCACTGA ATGCCGGATA CGCCGACGAC CAGCGGCGTC ACTCCATCGA GGTTTGCCTC 
CCAAGGGCTG GAACAACCAG CTCTAACGAA CACTTCACAG AGGAGGCCTA CTGGCTGGAG 
AAAGGCGGTC CTGTGAGGGT GCAGTCTGTC AGGAGCAACC ACAGAAAGAA GAAGATGAGT 
CCTCCATGTA TATCCATTCA CCCACCCTCA GAGAGGGAGC ACGCTGAAAC CGCCTCGCCC 
CCCAAATTAT CTGATTGCAG CACGATGCTG AGACGCAGGA CACCCTCCTA CGACTTGACG 
CCACACATGC AGTCAAATAC AAACGCCCAA GACGCGTCAT CAGATGCAAA CACGCAATCT 
CCAATGCACA GATCGCTAAC GCCTGTCCAT GTTCCTCTGC GAGCAAACTC GCCCACTTGG 
GCACTGGCTC ACTCGCGACC GTCTACCCCA ACACGCACTC AGACACACAC CCCTCCCCAC 
GCCTACACGC CTACACACCC ACACATCCCC ATCAGCCCGA ACGTCTTTAT CCAGCCTCAC 
ACCCCTCTTG TCCCAATGCC TTTCTCACAG ACACGCCCTG TCTCCCCTGC TGCGGGGCAC 
ATCCCCCTCC CTCAGTTCAC CTTCGACCAA CCGCAGTCCA GATACATGAG CGGCCTATCA 
GCTGGCATAT CAGACACCGA CACGGCCACC TGCTCTTCCC CTTTCGACGG CAGACTGGGA 
AGCGGCGCAG GATTCAGTGC AAAGAATCGG AGGACCGCCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777170.2
CDS	371..7975
Translation	MTDGEGRARFQYAECQELEEVREEVRLAVPTSYRNDGQGHEDDPGRRRPDDEEEEEEVERTRRSSRRRRRRSSSSSIQGDGENEWSPEVQGDEEQEPELEVEDGPTLGVRITAPIRDPDCVSEEEEQQPYPALAPVAFFCLKQTTRPRNWCLRMVCNPYPLTCWKSDPGNRWFEHVSMLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGKQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRSKENEGAEAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECHRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVSVMSVHSQPCESHSLLQVPDISPVHPRSPPSLSLPRIAPPTPSPSPRALHRQVAVKVDSVESQGFEQQERTSSVHLPPLSPSQLPPHPSSLSSPLPASPSSPSAFPRYRPSPLPPLLSSPSSLPPPPSSPSLLLPPPPSPRAPLRPASLRRPRPPSAISLCDPADEEVYHITSSAGHRWWDGEGGGTESRVKVGRSHSLSPYTLPSALDSYSSTFSPPPPPLPPSSSRSALSLLGAGLKDRDFRKGFSIDNQGFLDKPPSLNAGYADDQRRHSIEVCLPRAGTTSSNEHFTEEAYWLEKGGPVRVQSVRSNHRKKKMSPPCISIHPPSEREHAETASPPKLSDCSTMLRRRTPSYDLTPHMQSNTNAQDASSDANTQSPMHRSLTPVHVPLRANSPTWALAHSRPSTPTRTQTHTPPHAYTPTHPHIPISPNVFIQPHTPLVPMPFSQTRPVSPAAGHIPLPQFTFDQPQSRYMSGLSAGISDTDTATCSSPFDGRLGSGAGFSAKNRRTAQ

Target ORF information:

RefSeq Version	XM_012921716.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921716.2

ORF Insert Sequence:

ATGACTGATG GCGAAGGGAG AGCGCGCTTT CAGTACGCAG AGTGCCAGGA GCTGGAGGAG 
GTGCGGGAGG AGGTAAGGCT TGCTGTACCG ACATCCTACA GGAATGACGG CCAAGGTCAT 
GAAGATGACC CAGGAAGAAG ACGCCCGGAT GATGAGGAGG AAGAGGAGGA GGTGGAGAGG 
ACGAGAAGGA GCAGCAGGAG GAGGAGGAGG AGGAGCAGCA GCAGTAGCAT TCAAGGAGAT 
GGAGAAAATG AGTGGAGTCC TGAAGTTCAG GGGGATGAAG AACAGGAACC GGAGCTGGAA 
GTGGAAGATG GACCAACACT GGGAGTTCGC ATCACGGCTC CCATCCGTGA TCCAGACTGC 
GTCTCCGAGG AGGAGGAACA GCAGCCGTAT CCGGCCCTGG CCCCCGTAGC CTTCTTCTGC 
TTAAAGCAAA CAACGAGGCC TCGCAACTGG TGTCTCCGCA TGGTCTGCAA TCCATATCCT 
TTAACATGCT GGAAATCTGA CCCTGGTAAT CGGTGGTTTG AGCACGTGTC CATGTTGGTG 
ATCCTGCTCA ACTGTGTGAC TCTGGGGATG TATCGGCCCT GTGAGGACGT TACCTGCCAG 
TCAGAGTGGT GTCGCATCTT ACAGGTGTTG GATGACTGCA TCTTCGCTTT CTTTGCGGTG 
GAAATGGTTA TCAAGATGGT GGCCCTGGGA ATTTTTGGCT CCAAGTGTTA CTTTGATGAC 
AAGTGGAATC AGCTTGACTT TGTCATTGTC ATGGCAGGGA TGTTGGAGTA TTCCCTGGAT 
GGCCACAATG CCAGTCTGTC AGCTATCCGT ACCGTCCGAG TACTCAGGCC ACTGCGGGCT 
ATCAACAGAG TACCAAGTAT GAGGATCTTG GTGACACTGC TGTTAGACAC TCTGCCGATG 
CTGGGAAATG TTCTGCTCCT CTGCTTCTTT GTCTTCTTCA TCTTCGGCAT TGTGGGAGTC 
CAGTTGTGGG AGGGACTGCT GCGCAATCGC TGCTTCCTGG GAGAGGACAT CGAAACAATG 
TACAACATGT CTTTAAATCC TTATTACATG TCTGAGGAAG GTGAAGACAG CCCGTTTATC 
TGCTCGGCTC CACGCGAAAA CGGGATGCGG CGTTGCAAAG ATGTGCCACC CTACTCTCAA 
GACGGGCTCG AATGCACATT GCCGGCTTCT GACCTGAGTT TTAGGAGTGT TGCCAGCGGT 
AACTTTTGTG TTAACTGGTA TCAGTACTAC AACGACTGCC GCCCAGGGGA GCTCAACCCT 
CACAGGGGAG CTGTCAACTT TGACAATATC GGCTATGCTT GGATAGCCAT ATTCCAGGTC 
ATCACACTGG AAGGCTGGGT AGACATCATG TACTATGTCA TGGATGCTCA CTCCTTCTAT 
AATTTTATAT ATTTTATACT GCTCATTATT GTGGGCTCTT TCTTCATGAT CAACTTGTGC 
CTCGTGGTCA TTGCCACCCA ATTCTCCGAG ACAAAGCAAA GGGAGAATGC ACTGATGCGG 
GAGCAGCGTG CTCGTTACAT GTCAAACGAC TCCACACTTG CCAGTTACAG CGAGCCGGGC 
TCGTGCTATG AAGAGATGCT ACGCTACATT AGCCATCTTT ACCGCAAGTT TACACGCAGG 
CTCCAGAGGA TATACTCTGG CTGGCACAGT AAACGTCGTA AAAAGGTGAA TCCTAATGGC 
AGCAGTGGAG GCAGTAATGG TGGAGGTAAT GGGCATGGCC ACGGTTCCAG CAGGGGCTGC 
GGAGGGTCAG GCCCAGGTAA CGCATTGTGG TTGAGGCCAA TCCATAACCT TTTACAACAC 
CATCAGCATC AGCACTGTCG TCTCAGCAAT GGCGGGCAGC ACACCGTCAG CGTGGCAACC 
ACAGAGCACG TGGACAGAGA AGGACACATC AAAGTCGGGG AGGAACTGGA GATGAAGTCG 
CTTACCGTTC GGAGGGGAAG TGCAAATGGA AACAACGATA ACTCGACCAC TGTCACCCAG 
CGCGTGGGCT CATTACTCGG GCGACTGAAC GGGGGGATGA ACTATCCCAC CATTCTGCCG 
TCTTTTGTGT GCAGCTATAG CAGCAAGACG CCACCCCCTC ACTCAAAGCA GTGCGGTTCT 
TGTCTGGAGC ATCCGCCGAA CCACCCGGCT TCTGAGCACG CCGAGACACT GACCAAGCTT 
TACCAGCTCA TGGGACAGCA CAGTCGCCAG CGGCTGCTCT ACCAGCTGGC GGGTATGGTG 
CCCAGCCCTC TTCTCTTGGA GCTACTTAGC TGCCCTTTGT GTGCCAGCAG CCTGGAGACC 
TTGGAGCTGG AACTGGGAGA CCTAGAGGGA GGCAAGGGAG AGGCAGATGG ACTACACGAC 
TTCCCTCAGG GGGCCGATGT GAGAGGAGTG AGGGGTCGGA GCAGACGCCG TGGGGTTGTT 
GAATACGAAA ATGGAGTGGT TCGTGCCTGG AGGGACCTCA GGGAAAAACT AAAGAGGATT 
GTGGAAAGTA AATACTTCAA CCGCGGGATC ATGATCGCTA TTCTTGTGAA TACTCTCAGC 
ATGGGAATAG AGTACCACGA GCAGCCCGAG GAACTGACTG ATGTGTTAGA GATCAGCAAC 
ATCGTTTTCA CCAGCATGTT TGTCCTGGAG ATGGGTTTCA TGCTGCTGGC TTTCGGCTTG 
TTTGGATACA TCAGGAACCC ATACAACATC TTTGACAGCA TCATTGTTAT CATCAGTGTG 
TGGGAGATAA TTGGCCAAGC AGACGGTGGG CTGTCAGTCC TGCGAACGTT TCGTCTGCTA 
CGAGTTCTGA AGCTGGTTCG CTTCCTACCC GCTCTGAGGA GACAACTCGT AGTGCTGATG 
AAGACAATGG ACAATGTGGC CACCTTCTGC ATGCTTCTCA TGCTTTTCAT CTTCACTTTT 
AGTATACTGG GCATGCATCT TTTTGGGTGT AAATTTAGTT TTCGCACAGA GAATGGAGAC 
ACCATCCCTG ACCGAAAGAA CTTTGACAGT CTGCTCTGGG CCATTGTCAC TGTGTTCCAG 
ATTCTTACCC AGGAGGACTG GAATGTGGTG TTGTATAACG GCATGGCTTC TACCTCTCCA 
TGGGCGGCTC TCTACTTCGT TGCTCTTATG ACATTTGGCA ACTATGTGCT CTTCAATTTA 
CTAGTGGCCA TCTTGGTTGA AGGCTTCCAG GCTGAGGGTG ACGCTAATCG GTCAGAGTCA 
GATGATGACA AGAAATCAGA CAACTTTGAT GAGGACGAGA AGATAGAACA GTTCAGAGAC 
ACAGAGCTAA AGCTCTCACT GATGATGACA GGTAATGGTC ACATTGACCC TCGTGGCTCT 
ATGGCTCCTC CCATAATCAT GCGCACAGCA GCCACACCCA TGCCTACACC TAAGAGCTCT 
CTTGGACCTG AGTCCATCCT CGGGGATGAG CTCATGTACA TAGACGGAGC ACCTCAATAT 
AGTGAGGTAG GACTGGGTTT TCCTGAGGCA GGGGTCGGTC ACCCAGAGTC CCGACGGGGA 
AGCTCAACAT CAGTGGAGCC ATACGATCCG CCTTCTCTGA GCCTCTCTAG TCCCGGGCGG 
CGTTCTCCTC TTCCTCCCCG TGGCGCTGGA AGCTCATGGG GAAGTCGACG CTCCAGCTGG 
AACAGCCTGG GAAGGGCACC GAGCCTCAAA AGGCGAGACA CCAGCGGAGA GCGGGAGAGC 
TTGTTGTCTG GGGAAGGTGG AGAGGATGAG GATGCCAGGG ATGGCAAAGT GGAGGCAGGG 
GGAAACAACA GACTAAAGCC GGGGTCCTTG GAACTGCTGC AGGCGTCTAC TCTCTGCCCT 
TCTGTGGTAG CAGACTATGG TGACTGCAAT GGGAGGACCG TGACCTACAA CCCGAACGTG 
AACTCCGACC CCAAAGAAGA CTCCTCCCCA GAGGACGATT TGGACGATGA TGCTTCTTTT 
TGCTACTGGA CCAGGAAGGA GTTCCATAAC ATGAAGCCCC GATGGTGCAA AGAGCATGAA 
GACTGGACGC TGTACCTGTT TTCTCCAGAG AATCCGTTCC GTATGTGGTG CCAGAAAGTG 
ATCTCTCACA GACTGTTTGA CCACATAGTT CTAGTTTTCA TCTTTCTCAA CTGCATCACC 
ATTGCTCTGG AAAGACCTGA TATAAAAAAT GACAGCACGG AGCGACATTT CCTGAGTGTG 
TCCAACTATA TTTTCACTGT GATCTTCCTA GCAGAGATGG CCATTAAGGT TGTAGCTCTT 
GGTTTCTGCT TTGGGAAGCA GAGCTACCTC CAGTCCAGTT GGAACATTCT GGATGGTGTA 
TTGGTGTTTG TGTCTCTGAT CGACATTTTG GTTTATCTGG CCTATCATGG TGAAAACAAG 
ATCCTGGGAA TTCTGAGAGT ACTTCGCCTG CTACGAACAC TTCGCCCACT GAGGGTGATC 
AGTCGAGCCC CAGGATTAAA GCTTGTTGTG GAGACTCTCA TCACCTCTCT TAGACCTATT 
GGGAACATTG TTTTGATATG CTGCGCCTTC TTCATTATCT TTGGAATCTT GGGGGTCCAG 
CTGTTCAAAG GGAAGTTCTA CCACTGTGAA GGTCTGGACA CAAGAAACAT CACCAACAAA 
TCAGACTGTC TGCAGGCTAA CTACCGCTGG ATACGAAAAA AGTACAACTT TGACAACCTG 
ATTCAGGCTC TGATGTCTTT GTTTGTGCTG TCGTGTAAGG ATGGCTGGGT TACCATCATG 
TATGATGGGC TGGATGCAGT AGGAGTGGAC CAACAGCCTA AAAGAAACAA CAATCCTTGG 
ATGCTCCTCT ACTTTATCTC CTTCCTGCTC ATCGTCAGCT TCTTCGTCCT TAATATGTTT 
GTTGGGGTTG TGGTGGAGAA TTTCCACAAG TGCCGACAGG ACCAGGAAGA GGAGGAGGCG 
CGCTTACGGG AAGAGAAGAG GCTCAAAATG AAAGAGAAAA AGCGCAGGAG TAAGGAGAAT 
GAAGGGGCTG AGGCCAAGCT CGGGCGCTAC TATGACGACT ACTCTCCCCT GCGTCTGTCC 
ATCCACTCGC TGTGTACCAG CCACCACCTG GACCTCTTCA TCACTTTTAT CATCTGCGTC 
AATGTCTTCA CCATGAGCAT AGAGCACTAT AAACAACCAG TGTATTTAGG AGAGGTTCTG 
AAGTACTGTA ACTACGTGTT TACCTGCATC TTCGTCGTTG AGGCCCTCCT CAAACTTGTG 
GCATTTGGCA TACACAGGTT CATCAGAGAC AGGTGGAATC AGCTGGATTT AGCTATAGTG 
GCATTGTCCA TCATGGGCAT CGTCCTGGAG GAGCTGAAAA TGAAAGGCAC ATTACCAATA 
AATCCAACCA TCATCAGGAT CATGAGAGTG CTCAGGATAG CACGAGTGCT GAAGCTTCTA 
AAGATGGCCA CAGGGATGAG AGCTCTGTTG GATACTGTCA TGCAAGCACT GCCGCAGGTG 
GGAAATCTTG GTCTTCTCTT CATGCTGCTC TTCTTCATCT ATGCTGCTCT GGGAGTGGAG 
CTCTTTGGCA AACTGGAGTG CAGCGTCGAC AACCCATGTG AAGGTCTGGG TCGTCACGCA 
ACCTTTAGAA ATTTTGGGAT GGCCTTCCTC ACACTGTTTC GAGTGTCCAC TGGAGACAAC 
TGGAATGGGA TTATGAAAGA CACATTGAGA GAGTGTCACC GGTCTGACGA ACACTGTTTA 
CCATATCTGC CCTGGGTTTC TCCAATTTAC TTTGTCACCT TTGTGCTCAT GGCCCAGTTT 
GTTCTGGTCA ATGTGGTGGT GGCTGTTCTG ATGAAACATC TAGAGGAAAG CAACAAGGAT 
GCTAAAGAGG ATGCGGAGAT TGATGCAGAG ATCGCCTTGG AAATGGATAG GGAGAGGGAT 
CGCAGACTAA GCACAAATTC CAACAACAGC TCAGTCGGAG GGACCTATGT GGGGCCATGC 
TCAAATTCAC CTGAAGAGGA GGAAGAGGTG CAGTACAACA ACCAGAACCT GCTGTCATCC 
AAGATCTCTG TATCCAGGAT GCATTCTCTG CCAAATGACA GTTATATGTT TAGACCGGTG 
CGACCCGCCA GCGCCCCCTA CCCTTTAGAA GAGGTAGACC TGAGTCCCCA ACACCAGCAT 
TCAGTCTCAG TGATGTCGGT CCACTCCCAG CCCTGTGAGA GCCACTCTCT GCTCCAGGTT 
CCGGACATTT CCCCCGTTCA CCCTCGGTCA CCTCCATCCC TGTCTCTTCC TCGCATCGCC 
CCGCCAACAC CCAGCCCCTC ACCACGGGCC CTGCATAGGC AGGTGGCGGT TAAAGTTGAT 
TCTGTGGAGT CTCAGGGTTT TGAGCAACAG GAGAGGACCA GCTCAGTCCA TCTGCCCCCC 
CTGTCCCCTT CACAGCTACC TCCTCATCCA TCCTCCTTGT CATCACCCCT GCCTGCTTCT 
CCCTCCTCCC CCTCCGCCTT CCCTCGCTAT CGTCCTTCTC CTCTTCCTCC TCTGCTGTCT 
TCCCCTTCTT CTCTTCCTCC GCCACCGTCC TCCCCTTCGC TGCTTCTCCC TCCACCTCCC 
TCGCCCCGCG CCCCCTTGAG GCCTGCCAGC CTCCGGAGAC CAAGGCCGCC CTCCGCCATC 
TCCCTCTGTG ACCCGGCCGA TGAGGAGGTG TATCACATCA CCAGCTCTGC TGGCCACAGG 
TGGTGGGACG GGGAGGGAGG TGGGACGGAG AGCAGGGTTA AAGTGGGCCG CAGCCACTCC 
CTCTCCCCGT ACACCTTGCC GTCTGCCCTC GACTCCTACT CCTCCACCTT TTCTCCTCCT 
CCTCCTCCGC TGCCACCATC CTCGTCGAGG AGCGCACTGA GCCTGCTTGG AGCCGGACTG 
AAGGACCGGG ACTTCAGGAA AGGCTTCAGC ATCGACAACC AGGGCTTCCT GGACAAGCCG 
CCATCACTGA ATGCCGGATA CGCCGACGAC CAGCGGCGTC ACTCCATCGA GGTTTGCCTC 
CCAAGGGCTG GAACAACCAG CTCTAACGAA CACTTCACAG AGGAGGCCTA CTGGCTGGAG 
AAAGGCGGTC CTGTGAGGGT GCAGTCTGTC AGGAGCAACC ACAGAAAGAA GAAGATGAGT 
CCTCCATGTA TATCCATTCA CCCACCCTCA GAGAGGGAGC ACGCTGAAAC CGCCTCGCCC 
CCCAAATTAT CTGATTGCAG CACGATGCTG AGACGCAGGA CACCCTCCTA CGACTTGACG 
CCACACATGC AGTCAAATAC AAACGCCCAA GACGCGTCAT CAGATGCAAA CACGCAATCT 
CCAATGCACA GATCGCTAAC GCCTGTCCAT GTTCCTCTGC GAGCAAACTC GCCCACTTGG 
GCACTGGCTC ACTCGCGACC GTCTACCCCA ACACGCACTC AGACACACAC CCCTCCCCAC 
GCCTACACGC CTACACACCC ACACATCCCC ATCAGCCCGA ACGTCTTTAT CCAGCCTCAC 
ACCCCTCTTG TCCCAATGCC TTTCTCACAG ACACGCCCTG TCTCCCCTGC TGCGGGGCAC 
ATCCCCCTCC CTCAGTTCAC CTTCGACCAA CCGCAGTCCA GATACATGAG CGGCCTATCA 
GCTGGCATAT CAGACACCGA CACGGCCACC TGCTCTTCCC CTTTCGACGG CAGACTGGGA 
AGCGGCGCAG GATTCAGTGC AAAGAATCGG AGGACCGCCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101485565 ( XM_024802176.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_024657944.1 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X1, mRNA.

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CloneID	OMa371937
Clone ID Related Accession (Same CDS sequence)	XM_024802176.1
Accession Version	XM_024802176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTGCCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGCAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCGCCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCTCAGT GATGTCGGTC CACTCCCAGC CCTGTGAGAG CCACTCTCTG 
CTCCAGGTTC CGGACATTTC CCCCGTTCAC CCTCGGTCAC CTCCATCCCT GTCTCTTCCT 
CGCATCGCCC CGCCAACACC CAGCCCCTCA CCACGGGCCC TGCATAGGCA GGTGGCGGTT 
AAAGTTGATT CTGTGGAGTC TCAGGGTTTT GAGCAACAGG AGAGGACCAG CTCAGTCCAT 
CTGCCCCCCC TGTCCCCTTC ACAGCTACCT CCTCATCCAT CCTCCTTGTC ATCACCCCTG 
CCTGCTTCTC CCTCCTCCCC CTCCGCCTTC CCTCGCTATC GTCCTTCTCC TCTTCCTCCT 
CTGCTGTCTT CCCCTTCTTC TCTTCCTCCG CCACCGTCCT CCCCTTCGCT GCTTCTCCCT 
CCACCTCCCT CGCCCCGCGC CCCCTTGAGG CCTGCCAGCC TCCGGAGACC AAGGCCGCCC 
TCCGCCATCT CCCTCTGTGA CCCGGCCGAT GAGGAGGTGT ATCACATCAC CAGCTCTGCT 
GGCCACAGGT GGTGGGACGG GGAGGGAGGT GGGACGGAGA GCAGGGTTAA AGTGGGCCGC 
AGCCACTCCC TCTCCCCGTA CACCTTGCCG TCTGCCCTCG ACTCCTACTC CTCCACCTTT 
TCTCCTCCTC CTCCTCCGCT GCCACCATCC TCGTCGAGGA GCGCACTGAG CCTGCTTGGA 
GCCGGACTGA AGGACCGGGA CTTCAGGAAA GGCTTCAGCA TCGACAACCA GGGCTTCCTG 
GACAAGCCGC CATCACTGAA TGCCGGATAC GCCGACGACC AGCGGCGTCA CTCCATCGAG 
GTTTGCCTCC CAAGGGCTGG AACAACCAGC TCTAACGAAC ACTTCACAGA GGAGGCCTAC 
TGGCTGGAGA AAGGCGGTCC TGTGAGGGTG CAGTCTGTCA GGAGCAACCA CAGAAAGAAG 
AAGATGAGTC CTCCATGTAT ATCCATTCAC CCACCCTCAG AGAGGGAGCA CGCTGAAACC 
GCCTCGCCCC CCAAATTATC TGATTGCAGC ACGATGCTGA GACGCAGGAC ACCCTCCTAC 
GACTTGACGC CACACATGCA GTCAAATACA AACGCCCAAG ACGCGTCATC AGATGCAAAC 
ACGCAATCTC CAATGCACAG ATCGCTAACG CCTGTCCATG TTCCTCTGCG AGCAAACTCG 
CCCACTTGGG CACTGGCTCA CTCGCGACCG TCTACCCCAA CACGCACTCA GACACACACC 
CCTCCCCACG CCTACACGCC TACACACCCA CACATCCCCA TCAGCCCGAA CGTCTTTATC 
CAGCCTCACA CCCCTCTTGT CCCAATGCCT TTCTCACAGA CACGCCCTGT CTCCCCTGCT 
GCGGGGCACA TCCCCCTCCC TCAGTTCACC TTCGACCAAC CGCAGTCCAG ATACATGAGC 
GGCCTATCAG CTGGCATATC AGACACCGAC ACGGCCACCT GCTCTTCCCC TTTCGACGGC 
AGACTGGGAA GCGGCGCAGG ATTCAGTGCA AAGAATCGGA GGACCGCCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024657944.1
CDS	13..7086
Translation	MLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGQQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRSKENEGAEAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECRRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVSVMSVHSQPCESHSLLQVPDISPVHPRSPPSLSLPRIAPPTPSPSPRALHRQVAVKVDSVESQGFEQQERTSSVHLPPLSPSQLPPHPSSLSSPLPASPSSPSAFPRYRPSPLPPLLSSPSSLPPPPSSPSLLLPPPPSPRAPLRPASLRRPRPPSAISLCDPADEEVYHITSSAGHRWWDGEGGGTESRVKVGRSHSLSPYTLPSALDSYSSTFSPPPPPLPPSSSRSALSLLGAGLKDRDFRKGFSIDNQGFLDKPPSLNAGYADDQRRHSIEVCLPRAGTTSSNEHFTEEAYWLEKGGPVRVQSVRSNHRKKKMSPPCISIHPPSEREHAETASPPKLSDCSTMLRRRTPSYDLTPHMQSNTNAQDASSDANTQSPMHRSLTPVHVPLRANSPTWALAHSRPSTPTRTQTHTPPHAYTPTHPHIPISPNVFIQPHTPLVPMPFSQTRPVSPAAGHIPLPQFTFDQPQSRYMSGLSAGISDTDTATCSSPFDGRLGSGAGFSAKNRRTAQ

Target ORF information:

RefSeq Version	XM_024802176.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024802176.1

ORF Insert Sequence:

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTGCCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGCAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAGT 
AAGGAGAATG AAGGGGCTGA GGCCAAGCTC GGGCGCTACT ATGACGACTA CTCTCCCCTG 
CGTCTGTCCA TCCACTCGCT GTGTACCAGC CACCACCTGG ACCTCTTCAT CACTTTTATC 
ATCTGCGTCA ATGTCTTCAC CATGAGCATA GAGCACTATA AACAACCAGT GTATTTAGGA 
GAGGTTCTGA AGTACTGTAA CTACGTGTTT ACCTGCATCT TCGTCGTTGA GGCCCTCCTC 
AAACTTGTGG CATTTGGCAT ACACAGGTTC ATCAGAGACA GGTGGAATCA GCTGGATTTA 
GCTATAGTGG CATTGTCCAT CATGGGCATC GTCCTGGAGG AGCTGAAAAT GAAAGGCACA 
TTACCAATAA ATCCAACCAT CATCAGGATC ATGAGAGTGC TCAGGATAGC ACGAGTGCTG 
AAGCTTCTAA AGATGGCCAC AGGGATGAGA GCTCTGTTGG ATACTGTCAT GCAAGCACTG 
CCGCAGGTGG GAAATCTTGG TCTTCTCTTC ATGCTGCTCT TCTTCATCTA TGCTGCTCTG 
GGAGTGGAGC TCTTTGGCAA ACTGGAGTGC AGCGTCGACA ACCCATGTGA AGGTCTGGGT 
CGTCACGCAA CCTTTAGAAA TTTTGGGATG GCCTTCCTCA CACTGTTTCG AGTGTCCACT 
GGAGACAACT GGAATGGGAT TATGAAAGAC ACATTGAGAG AGTGTCGCCG GTCTGACGAA 
CACTGTTTAC CATATCTGCC CTGGGTTTCT CCAATTTACT TTGTCACCTT TGTGCTCATG 
GCCCAGTTTG TTCTGGTCAA TGTGGTGGTG GCTGTTCTGA TGAAACATCT AGAGGAAAGC 
AACAAGGATG CTAAAGAGGA TGCGGAGATT GATGCAGAGA TCGCCTTGGA AATGGATAGG 
GAGAGGGATC GCAGACTAAG CACAAATTCC AACAACAGCT CAGTCGGAGG GACCTATGTG 
GGGCCATGCT CAAATTCACC TGAAGAGGAG GAAGAGGTGC AGTACAACAA CCAGAACCTG 
CTGTCATCCA AGATCTCTGT ATCCAGGATG CATTCTCTGC CAAATGACAG TTATATGTTT 
AGACCGGTGC GACCCGCCAG CGCCCCCTAC CCTTTAGAAG AGGTAGACCT GAGTCCCCAA 
CACCAGCATT CAGTCTCAGT GATGTCGGTC CACTCCCAGC CCTGTGAGAG CCACTCTCTG 
CTCCAGGTTC CGGACATTTC CCCCGTTCAC CCTCGGTCAC CTCCATCCCT GTCTCTTCCT 
CGCATCGCCC CGCCAACACC CAGCCCCTCA CCACGGGCCC TGCATAGGCA GGTGGCGGTT 
AAAGTTGATT CTGTGGAGTC TCAGGGTTTT GAGCAACAGG AGAGGACCAG CTCAGTCCAT 
CTGCCCCCCC TGTCCCCTTC ACAGCTACCT CCTCATCCAT CCTCCTTGTC ATCACCCCTG 
CCTGCTTCTC CCTCCTCCCC CTCCGCCTTC CCTCGCTATC GTCCTTCTCC TCTTCCTCCT 
CTGCTGTCTT CCCCTTCTTC TCTTCCTCCG CCACCGTCCT CCCCTTCGCT GCTTCTCCCT 
CCACCTCCCT CGCCCCGCGC CCCCTTGAGG CCTGCCAGCC TCCGGAGACC AAGGCCGCCC 
TCCGCCATCT CCCTCTGTGA CCCGGCCGAT GAGGAGGTGT ATCACATCAC CAGCTCTGCT 
GGCCACAGGT GGTGGGACGG GGAGGGAGGT GGGACGGAGA GCAGGGTTAA AGTGGGCCGC 
AGCCACTCCC TCTCCCCGTA CACCTTGCCG TCTGCCCTCG ACTCCTACTC CTCCACCTTT 
TCTCCTCCTC CTCCTCCGCT GCCACCATCC TCGTCGAGGA GCGCACTGAG CCTGCTTGGA 
GCCGGACTGA AGGACCGGGA CTTCAGGAAA GGCTTCAGCA TCGACAACCA GGGCTTCCTG 
GACAAGCCGC CATCACTGAA TGCCGGATAC GCCGACGACC AGCGGCGTCA CTCCATCGAG 
GTTTGCCTCC CAAGGGCTGG AACAACCAGC TCTAACGAAC ACTTCACAGA GGAGGCCTAC 
TGGCTGGAGA AAGGCGGTCC TGTGAGGGTG CAGTCTGTCA GGAGCAACCA CAGAAAGAAG 
AAGATGAGTC CTCCATGTAT ATCCATTCAC CCACCCTCAG AGAGGGAGCA CGCTGAAACC 
GCCTCGCCCC CCAAATTATC TGATTGCAGC ACGATGCTGA GACGCAGGAC ACCCTCCTAC 
GACTTGACGC CACACATGCA GTCAAATACA AACGCCCAAG ACGCGTCATC AGATGCAAAC 
ACGCAATCTC CAATGCACAG ATCGCTAACG CCTGTCCATG TTCCTCTGCG AGCAAACTCG 
CCCACTTGGG CACTGGCTCA CTCGCGACCG TCTACCCCAA CACGCACTCA GACACACACC 
CCTCCCCACG CCTACACGCC TACACACCCA CACATCCCCA TCAGCCCGAA CGTCTTTATC 
CAGCCTCACA CCCCTCTTGT CCCAATGCCT TTCTCACAGA CACGCCCTGT CTCCCCTGCT 
GCGGGGCACA TCCCCCTCCC TCAGTTCACC TTCGACCAAC CGCAGTCCAG ATACATGAGC 
GGCCTATCAG CTGGCATATC AGACACCGAC ACGGCCACCT GCTCTTCCCC TTTCGACGGC 
AGACTGGGAA GCGGCGCAGG ATTCAGTGCA AAGAATCGGA GGACCGCCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101485565 ( XM_024802177.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_024657945.1 Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X2, mRNA.

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CloneID	OMa371939
Clone ID Related Accession (Same CDS sequence)	XM_024802177.1
Accession Version	XM_024802177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTGCCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGCAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAAG 
GCCAAGCTCG GGCGCTACTA TGACGACTAC TCTCCCCTGC GTCTGTCCAT CCACTCGCTG 
TGTACCAGCC ACCACCTGGA CCTCTTCATC ACTTTTATCA TCTGCGTCAA TGTCTTCACC 
ATGAGCATAG AGCACTATAA ACAACCAGTG TATTTAGGAG AGGTTCTGAA GTACTGTAAC 
TACGTGTTTA CCTGCATCTT CGTCGTTGAG GCCCTCCTCA AACTTGTGGC ATTTGGCATA 
CACAGGTTCA TCAGAGACAG GTGGAATCAG CTGGATTTAG CTATAGTGGC ATTGTCCATC 
ATGGGCATCG TCCTGGAGGA GCTGAAAATG AAAGGCACAT TACCAATAAA TCCAACCATC 
ATCAGGATCA TGAGAGTGCT CAGGATAGCA CGAGTGCTGA AGCTTCTAAA GATGGCCACA 
GGGATGAGAG CTCTGTTGGA TACTGTCATG CAAGCACTGC CGCAGGTGGG AAATCTTGGT 
CTTCTCTTCA TGCTGCTCTT CTTCATCTAT GCTGCTCTGG GAGTGGAGCT CTTTGGCAAA 
CTGGAGTGCA GCGTCGACAA CCCATGTGAA GGTCTGGGTC GTCACGCAAC CTTTAGAAAT 
TTTGGGATGG CCTTCCTCAC ACTGTTTCGA GTGTCCACTG GAGACAACTG GAATGGGATT 
ATGAAAGACA CATTGAGAGA GTGTCGCCGG TCTGACGAAC ACTGTTTACC ATATCTGCCC 
TGGGTTTCTC CAATTTACTT TGTCACCTTT GTGCTCATGG CCCAGTTTGT TCTGGTCAAT 
GTGGTGGTGG CTGTTCTGAT GAAACATCTA GAGGAAAGCA ACAAGGATGC TAAAGAGGAT 
GCGGAGATTG ATGCAGAGAT CGCCTTGGAA ATGGATAGGG AGAGGGATCG CAGACTAAGC 
ACAAATTCCA ACAACAGCTC AGTCGGAGGG ACCTATGTGG GGCCATGCTC AAATTCACCT 
GAAGAGGAGG AAGAGGTGCA GTACAACAAC CAGAACCTGC TGTCATCCAA GATCTCTGTA 
TCCAGGATGC ATTCTCTGCC AAATGACAGT TATATGTTTA GACCGGTGCG ACCCGCCAGC 
GCCCCCTACC CTTTAGAAGA GGTAGACCTG AGTCCCCAAC ACCAGCATTC AGTCTCAGTG 
ATGTCGGTCC ACTCCCAGCC CTGTGAGAGC CACTCTCTGC TCCAGGTTCC GGACATTTCC 
CCCGTTCACC CTCGGTCACC TCCATCCCTG TCTCTTCCTC GCATCGCCCC GCCAACACCC 
AGCCCCTCAC CACGGGCCCT GCATAGGCAG GTGGCGGTTA AAGTTGATTC TGTGGAGTCT 
CAGGGTTTTG AGCAACAGGA GAGGACCAGC TCAGTCCATC TGCCCCCCCT GTCCCCTTCA 
CAGCTACCTC CTCATCCATC CTCCTTGTCA TCACCCCTGC CTGCTTCTCC CTCCTCCCCC 
TCCGCCTTCC CTCGCTATCG TCCTTCTCCT CTTCCTCCTC TGCTGTCTTC CCCTTCTTCT 
CTTCCTCCGC CACCGTCCTC CCCTTCGCTG CTTCTCCCTC CACCTCCCTC GCCCCGCGCC 
CCCTTGAGGC CTGCCAGCCT CCGGAGACCA AGGCCGCCCT CCGCCATCTC CCTCTGTGAC 
CCGGCCGATG AGGAGGTGTA TCACATCACC AGCTCTGCTG GCCACAGGTG GTGGGACGGG 
GAGGGAGGTG GGACGGAGAG CAGGGTTAAA GTGGGCCGCA GCCACTCCCT CTCCCCGTAC 
ACCTTGCCGT CTGCCCTCGA CTCCTACTCC TCCACCTTTT CTCCTCCTCC TCCTCCGCTG 
CCACCATCCT CGTCGAGGAG CGCACTGAGC CTGCTTGGAG CCGGACTGAA GGACCGGGAC 
TTCAGGAAAG GCTTCAGCAT CGACAACCAG GGCTTCCTGG ACAAGCCGCC ATCACTGAAT 
GCCGGATACG CCGACGACCA GCGGCGTCAC TCCATCGAGG TTTGCCTCCC AAGGGCTGGA 
ACAACCAGCT CTAACGAACA CTTCACAGAG GAGGCCTACT GGCTGGAGAA AGGCGGTCCT 
GTGAGGGTGC AGTCTGTCAG GAGCAACCAC AGAAAGAAGA AGATGAGTCC TCCATGTATA 
TCCATTCACC CACCCTCAGA GAGGGAGCAC GCTGAAACCG CCTCGCCCCC CAAATTATCT 
GATTGCAGCA CGATGCTGAG ACGCAGGACA CCCTCCTACG ACTTGACGCC ACACATGCAG 
TCAAATACAA ACGCCCAAGA CGCGTCATCA GATGCAAACA CGCAATCTCC AATGCACAGA 
TCGCTAACGC CTGTCCATGT TCCTCTGCGA GCAAACTCGC CCACTTGGGC ACTGGCTCAC 
TCGCGACCGT CTACCCCAAC ACGCACTCAG ACACACACCC CTCCCCACGC CTACACGCCT 
ACACACCCAC ACATCCCCAT CAGCCCGAAC GTCTTTATCC AGCCTCACAC CCCTCTTGTC 
CCAATGCCTT TCTCACAGAC ACGCCCTGTC TCCCCTGCTG CGGGGCACAT CCCCCTCCCT 
CAGTTCACCT TCGACCAACC GCAGTCCAGA TACATGAGCG GCCTATCAGC TGGCATATCA 
GACACCGACA CGGCCACCTG CTCTTCCCCT TTCGACGGCA GACTGGGAAG CGGCGCAGGA 
TTCAGTGCAA AGAATCGGAG GACCGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024657945.1
CDS	13..7065
Translation	MLVILLNCVTLGMYRPCEDVTCQSEWCRILQVLDDCIFAFFAVEMVIKMVALGIFGSKCYFDDKWNQLDFVIVMAGMLEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGLLRNRCFLGEDIETMYNMSLNPYYMSEEGEDSPFICSAPRENGMRRCKDVPPYSQDGLECTLPASDLSFRSVASGNFCVNWYQYYNDCRPGELNPHRGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSNDSTLASYSEPGSCYEEMLRYISHLYRKFTRRLQRIYSGWHSKRRKKVNPNGSSGGSNGGGNGHGHGSSRGCGGSGPGNALWLRPIHNLLQHHQHQHCRLSNGGQHTVSVATTEHVDREGHIKVGEELEMKSLTVRRGSANGNNDNSTTVTQRVGSLLGRLNGGMNYPTILPSFVCSYSSKTPPPHSKQCGSCLEHPPNHPASEHAETLTKLYQLMGQHSRQRLLYQLAGMVPSPLLLELLSCPLCASSLETLELELGDLEGGKGEADGLHDFPQGADVRGVRGRSRRRGVVEYENGVVRAWRDLREKLKRIVESKYFNRGIMIAILVNTLSMGIEYHEQPEELTDVLEISNIVFTSMFVLEMGFMLLAFGLFGYIRNPYNIFDSIIVIISVWEIIGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSFRTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESDDDKKSDNFDEDEKIEQFRDTELKLSLMMTGNGHIDPRGSMAPPIIMRTAATPMPTPKSSLGPESILGDELMYIDGAPQYSEVGLGFPEAGVGHPESRRGSSTSVEPYDPPSLSLSSPGRRSPLPPRGAGSSWGSRRSSWNSLGRAPSLKRRDTSGERESLLSGEGGEDEDARDGKVEAGGNNRLKPGSLELLQASTLCPSVVADYGDCNGRTVTYNPNVNSDPKEDSSPEDDLDDDASFCYWTRKEFHNMKPRWCKEHEDWTLYLFSPENPFRMWCQKVISHRLFDHIVLVFIFLNCITIALERPDIKNDSTERHFLSVSNYIFTVIFLAEMAIKVVALGFCFGQQSYLQSSWNILDGVLVFVSLIDILVYLAYHGENKILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLQANYRWIRKKYNFDNLIQALMSLFVLSCKDGWVTIMYDGLDAVGVDQQPKRNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRLKMKEKKRRKAKLGRYYDDYSPLRLSIHSLCTSHHLDLFITFIICVNVFTMSIEHYKQPVYLGEVLKYCNYVFTCIFVVEALLKLVAFGIHRFIRDRWNQLDLAIVALSIMGIVLEELKMKGTLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSVDNPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRECRRSDEHCLPYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKDAKEDAEIDAEIALEMDRERDRRLSTNSNNSSVGGTYVGPCSNSPEEEEEVQYNNQNLLSSKISVSRMHSLPNDSYMFRPVRPASAPYPLEEVDLSPQHQHSVSVMSVHSQPCESHSLLQVPDISPVHPRSPPSLSLPRIAPPTPSPSPRALHRQVAVKVDSVESQGFEQQERTSSVHLPPLSPSQLPPHPSSLSSPLPASPSSPSAFPRYRPSPLPPLLSSPSSLPPPPSSPSLLLPPPPSPRAPLRPASLRRPRPPSAISLCDPADEEVYHITSSAGHRWWDGEGGGTESRVKVGRSHSLSPYTLPSALDSYSSTFSPPPPPLPPSSSRSALSLLGAGLKDRDFRKGFSIDNQGFLDKPPSLNAGYADDQRRHSIEVCLPRAGTTSSNEHFTEEAYWLEKGGPVRVQSVRSNHRKKKMSPPCISIHPPSEREHAETASPPKLSDCSTMLRRRTPSYDLTPHMQSNTNAQDASSDANTQSPMHRSLTPVHVPLRANSPTWALAHSRPSTPTRTQTHTPPHAYTPTHPHIPISPNVFIQPHTPLVPMPFSQTRPVSPAAGHIPLPQFTFDQPQSRYMSGLSAGISDTDTATCSSPFDGRLGSGAGFSAKNRRTAQ

Target ORF information:

RefSeq Version	XM_024802177.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra voltage-dependent T-type calcium channel subunit alpha-1I (LOC101485565), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024802177.1

ORF Insert Sequence:

ATGTTGGTGA TCCTGCTCAA CTGTGTGACT CTGGGGATGT ATCGGCCCTG TGAGGACGTT 
ACCTGCCAGT CAGAGTGGTG TCGCATCTTA CAGGTGTTGG ATGACTGCAT CTTCGCTTTC 
TTTGCGGTGG AAATGGTTAT CAAGATGGTG GCCCTGGGAA TTTTTGGCTC CAAGTGTTAC 
TTTGATGACA AGTGGAATCA GCTTGACTTT GTCATTGTCA TGGCAGGGAT GTTGGAGTAT 
TCCCTGGATG GCCACAATGC CAGTCTGTCA GCTATCCGTA CCGTCCGAGT ACTCAGGCCA 
CTGCGGGCTA TCAACAGAGT ACCAAGTATG AGGATCTTGG TGACACTGCT GTTAGACACT 
CTGCCGATGC TGGGAAATGT TCTGCTCCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATT 
GTGGGAGTCC AGTTGTGGGA GGGACTGCTG CGCAATCGCT GCTTCCTGGG AGAGGACATC 
GAAACAATGT ACAACATGTC TTTAAATCCT TATTACATGT CTGAGGAAGG TGAAGACAGC 
CCGTTTATCT GCTCGGCTCC ACGCGAAAAC GGGATGCGGC GTTGCAAAGA TGTGCCACCC 
TACTCTCAAG ACGGGCTCGA ATGCACATTG CCGGCTTCTG ACCTGAGTTT TAGGAGTGTT 
GCCAGCGGTA ACTTTTGTGT TAACTGGTAT CAGTACTACA ACGACTGCCG CCCAGGGGAG 
CTCAACCCTC ACAGGGGAGC TGTCAACTTT GACAATATCG GCTATGCTTG GATAGCCATA 
TTCCAGGTCA TCACACTGGA AGGCTGGGTA GACATCATGT ACTATGTCAT GGATGCTCAC 
TCCTTCTATA ATTTTATATA TTTTATACTG CTCATTATTG TGGGCTCTTT CTTCATGATC 
AACTTGTGCC TCGTGGTCAT TGCCACCCAA TTCTCCGAGA CAAAGCAAAG GGAGAATGCA 
CTGATGCGGG AGCAGCGTGC TCGTTACATG TCAAACGACT CCACACTTGC CAGTTACAGC 
GAGCCGGGCT CGTGCTATGA AGAGATGCTA CGCTACATTA GCCATCTTTA CCGCAAGTTT 
ACACGCAGGC TCCAGAGGAT ATACTCTGGC TGGCACAGTA AACGTCGTAA AAAGGTGAAT 
CCTAATGGCA GCAGTGGAGG CAGTAATGGT GGAGGTAATG GGCATGGCCA CGGTTCCAGC 
AGGGGCTGCG GAGGGTCAGG CCCAGGTAAC GCATTGTGGT TGAGGCCAAT CCATAACCTT 
TTACAACACC ATCAGCATCA GCACTGTCGT CTCAGCAATG GCGGGCAGCA CACCGTCAGC 
GTGGCAACCA CAGAGCACGT GGACAGAGAA GGACACATCA AAGTCGGGGA GGAACTGGAG 
ATGAAGTCGC TTACCGTTCG GAGGGGAAGT GCAAATGGAA ACAACGATAA CTCGACCACT 
GTCACCCAGC GCGTGGGCTC ATTACTCGGG CGACTGAACG GGGGGATGAA CTATCCCACC 
ATTCTGCCGT CTTTTGTGTG CAGCTATAGC AGCAAGACGC CACCCCCTCA CTCAAAGCAG 
TGCGGTTCTT GTCTGGAGCA TCCGCCGAAC CACCCGGCTT CTGAGCACGC CGAGACACTG 
ACCAAGCTTT ACCAGCTCAT GGGACAGCAC AGTCGCCAGC GGCTGCTCTA CCAGCTGGCG 
GGTATGGTGC CCAGCCCTCT TCTCTTGGAG CTACTTAGCT GCCCTTTGTG TGCCAGCAGC 
CTGGAGACCT TGGAGCTGGA ACTGGGAGAC CTAGAGGGAG GCAAGGGAGA GGCAGATGGA 
CTACACGACT TCCCTCAGGG GGCCGATGTG AGAGGAGTGA GGGGTCGGAG CAGACGCCGT 
GGGGTTGTTG AATACGAAAA TGGAGTGGTT CGTGCCTGGA GGGACCTCAG GGAAAAACTA 
AAGAGGATTG TGGAAAGTAA ATACTTCAAC CGCGGGATCA TGATCGCTAT TCTTGTGAAT 
ACTCTCAGCA TGGGAATAGA GTACCACGAG CAGCCCGAGG AACTGACTGA TGTGTTAGAG 
ATCAGCAACA TCGTTTTCAC CAGCATGTTT GTCCTGGAGA TGGGTTTCAT GCTGCTGGCT 
TTCGGCTTGT TTGGATACAT CAGGAACCCA TACAACATCT TTGACAGCAT CATTGTTATC 
ATCAGTGTGT GGGAGATAAT TGGCCAAGCA GACGGTGGGC TGTCAGTCCT GCGAACGTTT 
CGTCTGCTAC GAGTTCTGAA GCTGGTTCGC TTCCTGCCCG CTCTGAGGAG ACAACTCGTA 
GTGCTGATGA AGACAATGGA CAATGTGGCC ACCTTCTGCA TGCTTCTCAT GCTTTTCATC 
TTCACTTTTA GTATACTGGG CATGCATCTT TTTGGGTGTA AATTTAGTTT TCGCACAGAG 
AATGGAGACA CCATCCCTGA CCGAAAGAAC TTTGACAGTC TGCTCTGGGC CATTGTCACT 
GTGTTCCAGA TTCTTACCCA GGAGGACTGG AATGTGGTGT TGTATAACGG CATGGCTTCT 
ACCTCTCCAT GGGCGGCTCT CTACTTCGTT GCTCTTATGA CATTTGGCAA CTATGTGCTC 
TTCAATTTAC TAGTGGCCAT CTTGGTTGAA GGCTTCCAGG CTGAGGGTGA CGCTAATCGG 
TCAGAGTCAG ATGATGACAA GAAATCAGAC AACTTTGATG AGGACGAGAA GATAGAACAG 
TTCAGAGACA CAGAGCTAAA GCTCTCACTG ATGATGACAG GTAATGGTCA CATTGACCCT 
CGTGGCTCTA TGGCTCCTCC CATAATCATG CGCACAGCAG CCACACCCAT GCCTACACCT 
AAGAGCTCTC TTGGACCTGA GTCCATCCTC GGGGATGAGC TCATGTACAT AGACGGAGCA 
CCTCAATATA GTGAGGTAGG ACTGGGTTTT CCTGAGGCAG GGGTCGGTCA CCCAGAGTCC 
CGACGGGGAA GCTCAACATC AGTGGAGCCA TACGATCCGC CTTCTCTGAG CCTCTCTAGT 
CCCGGGCGGC GTTCTCCTCT TCCTCCCCGT GGCGCTGGAA GCTCATGGGG AAGTCGACGC 
TCCAGCTGGA ACAGCCTGGG AAGGGCACCG AGCCTCAAAA GGCGAGACAC CAGCGGAGAG 
CGGGAGAGCT TGTTGTCTGG GGAAGGTGGA GAGGATGAGG ATGCCAGGGA TGGCAAAGTG 
GAGGCAGGGG GAAACAACAG ACTAAAGCCG GGGTCCTTGG AACTGCTGCA GGCGTCTACT 
CTCTGCCCTT CTGTGGTAGC AGACTATGGT GACTGCAATG GGAGGACCGT GACCTACAAC 
CCGAACGTGA ACTCCGACCC CAAAGAAGAC TCCTCCCCAG AGGACGATTT GGACGATGAT 
GCTTCTTTTT GCTACTGGAC CAGGAAGGAG TTCCATAACA TGAAGCCCCG ATGGTGCAAA 
GAGCATGAAG ACTGGACGCT GTACCTGTTT TCTCCAGAGA ATCCGTTCCG TATGTGGTGC 
CAGAAAGTGA TCTCTCACAG ACTGTTTGAC CACATAGTTC TAGTTTTCAT CTTTCTCAAC 
TGCATCACCA TTGCTCTGGA AAGACCTGAT ATAAAAAATG ACAGCACGGA GCGACATTTC 
CTGAGTGTGT CCAACTATAT TTTCACTGTG ATCTTCCTAG CAGAGATGGC CATTAAGGTT 
GTAGCTCTTG GTTTCTGCTT TGGGCAGCAG AGCTACCTCC AGTCCAGTTG GAACATTCTG 
GATGGTGTAT TGGTGTTTGT GTCTCTGATC GACATTTTGG TTTATCTGGC CTATCATGGT 
GAAAACAAGA TCCTGGGAAT TCTGAGAGTA CTTCGCCTGC TACGAACACT TCGCCCACTG 
AGGGTGATCA GTCGAGCCCC AGGATTAAAG CTTGTTGTGG AGACTCTCAT CACCTCTCTT 
AGACCTATTG GGAACATTGT TTTGATATGC TGCGCCTTCT TCATTATCTT TGGAATCTTG 
GGGGTCCAGC TGTTCAAAGG GAAGTTCTAC CACTGTGAAG GTCTGGACAC AAGAAACATC 
ACCAACAAAT CAGACTGTCT GCAGGCTAAC TACCGCTGGA TACGAAAAAA GTACAACTTT 
GACAACCTGA TTCAGGCTCT GATGTCTTTG TTTGTGCTGT CGTGTAAGGA TGGCTGGGTT 
ACCATCATGT ATGATGGGCT GGATGCAGTA GGAGTGGACC AACAGCCTAA AAGAAACAAC 
AATCCTTGGA TGCTCCTCTA CTTTATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTCCTT 
AATATGTTTG TTGGGGTTGT GGTGGAGAAT TTCCACAAGT GCCGACAGGA CCAGGAAGAG 
GAGGAGGCGC GCTTACGGGA AGAGAAGAGG CTCAAAATGA AAGAGAAAAA GCGCAGGAAG 
GCCAAGCTCG GGCGCTACTA TGACGACTAC TCTCCCCTGC GTCTGTCCAT CCACTCGCTG 
TGTACCAGCC ACCACCTGGA CCTCTTCATC ACTTTTATCA TCTGCGTCAA TGTCTTCACC 
ATGAGCATAG AGCACTATAA ACAACCAGTG TATTTAGGAG AGGTTCTGAA GTACTGTAAC 
TACGTGTTTA CCTGCATCTT CGTCGTTGAG GCCCTCCTCA AACTTGTGGC ATTTGGCATA 
CACAGGTTCA TCAGAGACAG GTGGAATCAG CTGGATTTAG CTATAGTGGC ATTGTCCATC 
ATGGGCATCG TCCTGGAGGA GCTGAAAATG AAAGGCACAT TACCAATAAA TCCAACCATC 
ATCAGGATCA TGAGAGTGCT CAGGATAGCA CGAGTGCTGA AGCTTCTAAA GATGGCCACA 
GGGATGAGAG CTCTGTTGGA TACTGTCATG CAAGCACTGC CGCAGGTGGG AAATCTTGGT 
CTTCTCTTCA TGCTGCTCTT CTTCATCTAT GCTGCTCTGG GAGTGGAGCT CTTTGGCAAA 
CTGGAGTGCA GCGTCGACAA CCCATGTGAA GGTCTGGGTC GTCACGCAAC CTTTAGAAAT 
TTTGGGATGG CCTTCCTCAC ACTGTTTCGA GTGTCCACTG GAGACAACTG GAATGGGATT 
ATGAAAGACA CATTGAGAGA GTGTCGCCGG TCTGACGAAC ACTGTTTACC ATATCTGCCC 
TGGGTTTCTC CAATTTACTT TGTCACCTTT GTGCTCATGG CCCAGTTTGT TCTGGTCAAT 
GTGGTGGTGG CTGTTCTGAT GAAACATCTA GAGGAAAGCA ACAAGGATGC TAAAGAGGAT 
GCGGAGATTG ATGCAGAGAT CGCCTTGGAA ATGGATAGGG AGAGGGATCG CAGACTAAGC 
ACAAATTCCA ACAACAGCTC AGTCGGAGGG ACCTATGTGG GGCCATGCTC AAATTCACCT 
GAAGAGGAGG AAGAGGTGCA GTACAACAAC CAGAACCTGC TGTCATCCAA GATCTCTGTA 
TCCAGGATGC ATTCTCTGCC AAATGACAGT TATATGTTTA GACCGGTGCG ACCCGCCAGC 
GCCCCCTACC CTTTAGAAGA GGTAGACCTG AGTCCCCAAC ACCAGCATTC AGTCTCAGTG 
ATGTCGGTCC ACTCCCAGCC CTGTGAGAGC CACTCTCTGC TCCAGGTTCC GGACATTTCC 
CCCGTTCACC CTCGGTCACC TCCATCCCTG TCTCTTCCTC GCATCGCCCC GCCAACACCC 
AGCCCCTCAC CACGGGCCCT GCATAGGCAG GTGGCGGTTA AAGTTGATTC TGTGGAGTCT 
CAGGGTTTTG AGCAACAGGA GAGGACCAGC TCAGTCCATC TGCCCCCCCT GTCCCCTTCA 
CAGCTACCTC CTCATCCATC CTCCTTGTCA TCACCCCTGC CTGCTTCTCC CTCCTCCCCC 
TCCGCCTTCC CTCGCTATCG TCCTTCTCCT CTTCCTCCTC TGCTGTCTTC CCCTTCTTCT 
CTTCCTCCGC CACCGTCCTC CCCTTCGCTG CTTCTCCCTC CACCTCCCTC GCCCCGCGCC 
CCCTTGAGGC CTGCCAGCCT CCGGAGACCA AGGCCGCCCT CCGCCATCTC CCTCTGTGAC 
CCGGCCGATG AGGAGGTGTA TCACATCACC AGCTCTGCTG GCCACAGGTG GTGGGACGGG 
GAGGGAGGTG GGACGGAGAG CAGGGTTAAA GTGGGCCGCA GCCACTCCCT CTCCCCGTAC 
ACCTTGCCGT CTGCCCTCGA CTCCTACTCC TCCACCTTTT CTCCTCCTCC TCCTCCGCTG 
CCACCATCCT CGTCGAGGAG CGCACTGAGC CTGCTTGGAG CCGGACTGAA GGACCGGGAC 
TTCAGGAAAG GCTTCAGCAT CGACAACCAG GGCTTCCTGG ACAAGCCGCC ATCACTGAAT 
GCCGGATACG CCGACGACCA GCGGCGTCAC TCCATCGAGG TTTGCCTCCC AAGGGCTGGA 
ACAACCAGCT CTAACGAACA CTTCACAGAG GAGGCCTACT GGCTGGAGAA AGGCGGTCCT 
GTGAGGGTGC AGTCTGTCAG GAGCAACCAC AGAAAGAAGA AGATGAGTCC TCCATGTATA 
TCCATTCACC CACCCTCAGA GAGGGAGCAC GCTGAAACCG CCTCGCCCCC CAAATTATCT 
GATTGCAGCA CGATGCTGAG ACGCAGGACA CCCTCCTACG ACTTGACGCC ACACATGCAG 
TCAAATACAA ACGCCCAAGA CGCGTCATCA GATGCAAACA CGCAATCTCC AATGCACAGA 
TCGCTAACGC CTGTCCATGT TCCTCTGCGA GCAAACTCGC CCACTTGGGC ACTGGCTCAC 
TCGCGACCGT CTACCCCAAC ACGCACTCAG ACACACACCC CTCCCCACGC CTACACGCCT 
ACACACCCAC ACATCCCCAT CAGCCCGAAC GTCTTTATCC AGCCTCACAC CCCTCTTGTC 
CCAATGCCTT TCTCACAGAC ACGCCCTGTC TCCCCTGCTG CGGGGCACAT CCCCCTCCCT 
CAGTTCACCT TCGACCAACC GCAGTCCAGA TACATGAGCG GCCTATCAGC TGGCATATCA 
GACACCGACA CGGCCACCTG CTCTTCCCCT TTCGACGGCA GACTGGGAAG CGGCGCAGGA 
TTCAGTGCAA AGAATCGGAG GACCGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

LOC101485565 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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